Journal of Immunological Methods

Papers
(The median citation count of Journal of Immunological Methods is 1. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-04-01 to 2025-04-01.)
ArticleCitations
Publisher's note: Challenges for complement functional assays in the clinical laboratory: From test validation to clinical interpretation58
Appropriate immunoassay systems are needed to evaluate various anticentromere antibodies as biomarkers for disease progression in systemic sclerosis55
Editorial Board34
A novel method for in vitro culture and expansion of nonhuman primate B cells29
Editorial Board25
Inter-intra instrument comparison and standardization of a 10-color immunophenotyping for B and T cell non-Hodgkin lymphoma diagnosis and monitoring21
Immunoglobulin isotype compositions of ABO specific antibodies are dependent on the individual patient blood group and blood group specificity: Results from a healthy donor cohort19
Role of antinuclear antibodies in COVID-19 patients18
A sensitive and less cytotoxic assay for identification of proliferating T cells based on bioorthogonally-functionalized uridine analogue18
Establishment of a novel cell-based assay using HLA-transfected cells to detect HLA antibodies18
Characterization and ex vivo expansion of rare in situ cytokine secreting T cell populations from tumor tissue and blood of oral squamous cell carcinoma patients16
Editorial Board16
A single homogeneous assay for simultaneous measurement of bispecific antibody target binding15
Comparability study of monocyte derived dendritic cells, primary monocytes, and THP1 cells for innate immune responses15
Thanking the reviewers of Journal of Immunological Methods14
Editorial Board14
A rapid and high-throughput T cell immunophenotyping assay for cellular therapy bioprocess using the Cellaca® PLX image cytometer14
An enzyme-free method for isolating testicular macrophages from rodent models13
Editorial Board13
Recombinant antigen 5 from Polybia paulista wasp venom (Hymenoptera, Vespidae): Antigen-specific antibody production and functional profile of CD4+ T cells in the immune response13
Editorial introduction for “B cell immunodeficiencies” Journal of Immunological Methods special edition13
Combined RNAscope and immunohistochemistry staining on duodenal paraffin sections as a new tool to reveal cytolytic potential of intraepithelial lymphocytes13
Editorial Board13
Detection of drug-specific immunoglobulin E (IgE) and acute mediator release for the diagnosis of immediate drug hypersensitivity reactions11
Development of competitive inhibition ELISA as an effective potency test to analyze human rabies vaccines and assessment of the antigenic epitope of rabies glycoprotein11
Quantitative determination of C-polysaccharide in Streptococcus pneumoniae capsular polysaccharides by enzyme-linked immunosorbent assay11
Feasibility of using monocyte-derived dendritic cells obtained from cryopreserved cells for DC-based vaccines11
Development of an indirect competitive enzyme linked immunosorbent assay for the quantitative detection of Mycoplasma hyopneumoniae during the vaccine production process10
Development of a flow cytometry-based potency assay for prediction of cytokine storms induced by biosimilar monoclonal antibodies10
Discrepancies between two total IgE assays and difference in reference intervals in healthy adults10
SARS-CoV-2 spike protein expression as an identification in quality control testing for Adenovector based COVID-19 vaccine10
Development of gold nanocluster complex for the detection of tumor necrosis factor-alpha based on immunoassay10
Development of a defibrinated human blood hemolysis assay for rapid testing of hemolytic activity compared to computational prediction10
Robust and low-cost ELISA based on IgG-Fc tagged recombinant proteins to screen for anti-SARS-CoV-2 antibodies10
Phospho-flow cytometry assays for diagnostic use – A discussion of assay utility and assay development and validation challenges10
Clinical utility of the lymphocyte proliferation assay, an in vitro functional readout of the adaptive immune response10
Evaluation of cell-mediated immunity of E.coli nanovaccines in chickens10
Challenges for complement functional assays in the clinical laboratory: From test validation to clinical interpretation10
The generation of a novel HER2-targeting nano PE25 immunotoxin with superior anti-tumor activity and high productivity9
Digital RT-PCR Chip method for detection of SARS-CoV-2 virus9
Parameters of immunoglobulin extraction from dried blood spot cards and immunoassays for detection of antibody response to pathogens including the novel SARS-CoV-29
Establishment of growth stimulating gene 2 protein time-resolved fluorescence immunoassay and its application in sepsis9
A sensitive method to quantify HIV-1 antibodies in mucosal samples9
Immunoassay for detection of oligomeric proteins9
Development of immunochromatographic strip assay to detect recent infection of Japanese encephalitis virus in swine population9
Selection of positive controls and their impact on anti-drug antibody assay performance9
Decreased variability in the site-specific results during participation in the External Quality Assurance Program Oversight Laboratory (EQAPOL) proficiency program for IFN-gamma enzyme-linked immunosp9
A chimeric HLA-A2:β2M:Ig fusion protein for the study of virus-specific CD8+ T-cells8
Establishment of a homogeneous immunoassay-light-initiated chemiluminescence assay for detecting anti-Müllerian hormone in human serum8
Validation of the performance and suitability of a new class of FBS optimized for use in single-cell functional assays8
Dried blood spots for the quantitative evaluation of IgG isotypes and correlation with serum samples in HIV-exposed uninfected (HEU) infants8
Immunodetection of Furcraea Necrotic Streak Virus-FNSV in fique plants (Furcraea macrophylla Baker) using a polyclonal antibody IgY produced in chicken egg yolk8
Construction of a full-length antibody phage display vector8
Phage display selection of human single domain antibodies towards karilysin, a metalloproteinase and secreted virulence factor from Tannerella forsythia8
Critical evaluation of fluorescent dyes to evaluate the stability and ligand binding properties of an anti-cocaine mAb, h2E28
Interactions of human mesenchymal stromal cells with peripheral blood mononuclear cells in a Mitogenic proliferation assay7
Optimization of lateral flow assay for Canine morbillivirus detection and the application of the strip as sample substitute7
Optimized cytotoxicity assay for co-suspended effector and target cells7
A SMART method for isolating monoclonal antibodies from individual rhesus macaque memory B cells7
A FluoroSpot B assay for the detection of IgA and IgG SARS-CoV-2 spike-specific memory B cells: Optimization and qualification for use in COVID-19 vaccine trials7
Comparison of methods to isolate peripheral blood mononuclear cells from cattle blood7
Comparison of single-chain variable fragments and monoclonal antibody against dihydroartemisinin7
Development of an ELISA for an effective potency determination of recombinant rabies human monoclonal antibody7
Development of a new automated IL-6 immunoassay6
Sensitive amplified luminescent proximity homogeneous assay for the quantitative detection of CA2426
Small p53 derived peptide suitable for robust nanobodies dimerization6
Candidate antibody reference reagents for Chlamydia trachomatis serology6
Agreement and error of titration assays6
30-color full spectrum flow cytometry panel for deep immunophenotyping of T cell subsets in murine tumor tissue6
A sensitive and drug tolerant assay for detecting anti-AAV9 antibodies using affinity capture elution6
Overview of human B-cell development and antibody deficiencies6
Isolation of phage-antibodies against Eimeria species that infect chickens6
Non-invasive image-based cytometry for high throughput NK cell cytolysis analysis6
Development of a rapid lateral flow immunochromatography assay for the detection of group-specific antibodies against VP7 protein of bluetongue virus in multiple species6
Stabilization of leukocytes from cerebrospinal fluid for central immunophenotypic evaluation in multicenter clinical trials6
Editorial – Immunobiophysics: Advances and techniques6
Development of ELISA for the detection of antibodies against VP2 protein of bluetongue virus serotype-15
SARS-CoV-2 serology testing: Progress and challenges5
Flow cytometry for B-cell subset analysis in immunodeficiencies5
Mapping antibody binding using multiplexed epitope substitution analysis5
Ultrasensitive multiplexed chemiluminescent enzyme-linked immunosorbent assays in 384-well plates5
Development of optimized cytotoxicity assays for assessing the antitumor potential of CAR-T cells5
Advanced hybridoma technology for selective production of high-affinity monoclonal antibodies through B-cell receptors5
Validation of a combined ELISA to detect IgG, IgA and IgM antibody responses to SARS-CoV-2 in mild or moderate non-hospitalised patients5
Optimization of IgG1 immune complexes to stimulate cytokine production in innate cells5
Rapid, sensitive and cost-effective determination of immune checkpoint inhibitor activity using a magnetic bead-based binding assay5
Artificial intelligence and deep learning to map immune cell types in inflamed human tissue5
Editorial Board5
Sources of variability in Luminex bead-based cytokine assays: Evidence from twelve years of multi-site proficiency testing5
Comprehensive phenotyping of circulating immune cell subsets in people living with HIV5
Techniques for evaluating the ATP-gated ion channel P2X7 receptor function in macrophages and microglial cells5
Evaluation of a multiplexed coronavirus antigen array for detection of SARS-CoV-2 specific IgG in COVID-19 convalescent plasma5
Optimisation and validation of a new method for antibody dependent cellular phagocytosis in hepatitis C virus infection5
Plaque reduction neutralization test for smallpox vaccines: Laboratory optimization and validation method for immunogenicity assessment5
Smartphone based lateral flow immunoassay quantifications5
Complete primer set for amplification and expression of full-length recombinant human monoclonal antibodies from single human B cells5
Optimized flow cytometry protocol for dihydrorhodamine 123-based detection of reactive oxygen species in leukocyte subpopulations in whole blood5
Editorial Board5
Comparison of hemagglutination inhibition tests, immunoperoxidase monolayer assays, and serum neutralizing tests in detecting antibodies against blue eye disease in pigs5
Rapid identification of tumor-reactive T-cell receptors by RNA preamplification-based single-cell sequencing5
Epitope profiling of cow's milk allergen-specific antibodies with determining IgE content in epitopes-ALL, a 14-epitopes mixture5
Editorial Board5
Development of rELISA using novel markers for the diagnosis of paratuberculosis5
Editorial Board5
Identification of the linear Fc-binding epitope on the bovine IgG1 Fc receptor of (boFcγRI) using synthetic peptides5
An easy assay to detect autoantibodies neutralizing cytokines in subjects with critical infections5
Diagnosis of septic shock by serum measurement of human neutrophil lipocalin by a rapid homogeneous assay4
The role of gamma globulin, complement component 1q, factor B, properdin, body temperature, C-reactive protein and serum amyloid alpha to the activity and the function of the human complement system a4
Comparative assessment of cytometry by time-of-flight and full spectral flow cytometry based on a 33-color antibody panel4
Editorial Board4
Reply to “Precision-cut human liver slice cultures as an immunological platform”4
ERAMER: A novel in silico tool for prediction of ERAP1 enzyme trimming4
Evaluation of antibody titer kinetics and SARS-CoV-2 infections in a large cohort of healthcare professionals ten months after administration of the BNT162b2 vaccine4
A novel whole blood assay to quantify the release of T cell associated cytokines in response to Bordetella pertussis antigens4
A novel antigen immunochromatography fluorometric strip for rapid detection and application of pathogenic bacterial high-quality antibody4
MILKSHAKE Western blot and Sundae ELISA: We all scream for better antibody validation4
Development and evaluation of a multi-antigen serological assay for the intra-vitam diagnosis of Tuberculosis caused by Mycobacterium bovis in pigs4
Retention of E-selectin functionalized liposome fanny packs on Jurkat cells following invasion through collagen4
Individual polysaccharide quantification in polyvalent pneumococcal conjugate vaccine using rate nephelometry4
Stability of anti-drug antibodies in human serum samples4
Generation of good manufacturing practice grade virus-specific T cells for the management of post-transplant CMV infections4
Highlights of the 33rd annual scientific meeting of the Association of Medical Laboratory Immunologists (AMLI)4
Methyl ester and aromatic ether modification of mitragynine for generation of mitragynine-specific polyclonal antibodies4
Novel immunoassay for diagnosis of ongoing Clostridioides difficile infections using serum and medium enriched for newly synthesized antibodies (MENSA)4
An eight-plex immunoassay for Group A streptococcus serology and vaccine development4
Editorial – Special Issue on in vitro detection of drug allergy4
Isolation of anti-Ancylostoma-secreted protein 5 (ASP5) antibody from a naïve antibody phage library4
Editorial Board4
Parameters of TNF receptor co-expression in allergic and autoimmune processes: Differences and diagnostic significance4
Establishment of bioluminescent imaging model using murine T cell lymphoma susceptive to NK cell-dependent immune-surveillance4
Whole blood based point-of-care assay for the detection of anti-pertussis toxin IgG antibodies4
Detection of RNA-dependent RNA polymerase of porcine epidemic diarrhea virus4
Fresh versus frozen PMBC using the SARS CoV-2 T-SPOT.COVID test. Which works best?4
A novel immuno-molecular strategy for the detection of Streptococcus pneumoniae serotypes in human cerebrospinal and middle ear fluids4
Immunoinformatics and Pepscan strategies on the path of a peptide-based serological diagnosis of COVID194
Mitochondrial function in oral health and disease3
Development and evaluation of a low cost IgG ELISA test based in RBD protein for COVID-193
Detection of alloreactive T cells from cryopreserved human peripheral blood mononuclear cells3
Tumor nano-lysate activates dendritic cells to evoke a preventative immune response3
Effect of wash media type during PBMC isolation on downstream characterization of SARS-CoV-2-specific T cells3
Development of a light-initiated chemiluminescence assay for the quantitative detection of cow's milk allergen-specific IgE3
Dissociation of murine oral mucosal tissues for single cell applications3
Evaluation of circulating leucocyte populations both in subjects with previous SARS-COV-2 infection and in healthy subjects after vaccination3
Development and validation of indirect ELISA for antibody detection against different protein antigens of porcine epidemic diarrhea virus in the colostrum and milk of sows3
Rapid, sensitive, and specific lateral-flow immunochromatographic point-of-care device for detection of varicella-zoster virus immunoglobulin G antibodies in fingerstick blood3
Identification of polyreactive antibodies by high throughput enzyme-linked immunosorbent assay and surface Plasmon resonance3
Comparison of two anti-histones antibodies commercial assays in an immunology laboratory and systemic lupus erythematosus3
Bacterial coloration immunofluorescence strip for ultrasensitive rapid detection of bacterial antibodies and targeted antibody-secreting hybridomas3
Letter to the editor regarding “Stabilization of leukocytes from cerebrospinal fluid for central immunophenotypic evaluation in multicenter clinical trials”3
High-yield production of recombinant human myelin oligodendrocyte glycoprotein in SHuffle bacteria without a refolding step3
Proficiency tests to evaluate the impact on assay outcomes of harmonized influenza-specific Intracellular Cytokine Staining (ICS) and IFN-ɣ Enzyme-Linked ImmunoSpot (ELISpot) protocols3
In vitro immunization approach to generate specific murine monoclonal IgG antibodies3
Human monoclonal antibody cloning and expression with overlap extension PCR and short DNA fragments3
Validation of dot blot immunoassay for measurement of complement opsonization of nanoparticles3
Development and qualification of a fast, high-throughput and robust imaging-based neutralization assay for respiratory syncytial virus3
The C-terminus of Brucella abortus MviN induces humoral and cell mediated immune responses in BALB/c mice that protects against the virulent Brucella 544 challenge3
Potential diagnostic application of the baculovirus-expressed recombinant truncated nucleocapsid protein of peste des petits ruminants virus in ELISA3
Highly versatile antibody binding assay for the detection of SARS-CoV-2 infection and vaccination3
Flow cytometric determination of ferroptosis using a rat monoclonal antibody raised against ferroptotic cells3
Optimisation of SARS-CoV-2 peptide stimulation and measurement of cytokine output by intracellular flow cytometry and bio-plex analysis3
Flow cytometry reveals constant lymphocyte proportions after long-term cryopreservation of whole blood in TransFix® cell stabilization reagent3
Novel hapten design, highly sensitive monoclonal antibody production, and immunoassay development for rapid screening of illegally added chloramphenicol in cosmetics3
Humoral and cellular response of two different vaccines against SARS-CoV-2 in a group of healthcare workers: Comment3
Monocyte monolayer assay in pre-transfusion testing: A magic key in transfusing patients with recurrent bad cross-match due to alloimmunization3
Comparative analysis of LAG3 antibodies shows differential binding patterns by flow cytometry3
In vitro differentiation of myeloid suppressor cells (MDSC-like) from an immature myelomonocytic precursor THP-13
Neuronal binding by antibodies can be influenced by low pH stress during the isolation procedure3
Fine definition of the epitopes on the human gp91 /NOX2 for the monoclonal antibodies CL-5 and 483
Surface Plasmon Resonance (SPR) biosensor for detection of mycotoxins: A review3
Functional assays for the diagnosis of chronic granulomatous disease3
Generation of an alpaca serum that induces immune-mediated crescentic glomerulonephritis in mice3
Electrofusion preparation of anti-triazophos monoclonal antibodies for development of an indirect competitive enzyme-linked immunosorbent assay3
Development of a non-biased, high-throughput ELISA for the rapid evaluation of immunogenicity and cross-reactivity3
Dried blood spots are a valid alternative to venipuncture for COVID-19 antibody testing3
Simultaneous determination of inflammatory factors SAA and LTF based on stable element labeling and inductively coupled plasma mass spectrometry to aid in the diagnosis of infection3
Assessing TCR identity, knock-in efficiency, and potency for individualized TCR-T cell therapy3
Immunofluorescence analysis of human eosinophils3
Validation of dried blood spots samples for the screening of hepatitis B virus surface by enzyme immunoassay method3
“Common variable immunodeficiency: Challenges for diagnosis”3
Differential composition and yield of leukocytes isolated from various blood component leukoreduction filters2
Optimizing the viability, stability, and potency of Buffy coat isolated T cells for homologous dendritic cell co-cultures: A method for handling and preservation2
Antibody detection by agglutination-PCR (ADAP) assays for the analysis of tissue transglutaminase autoantibodies in celiac disease2
Identification and characterization of phage display-selected peptides having affinity to Peste des petits ruminants virus2
Editorial Board2
New and rapid strategies for the diagnosis of bovine paratuberculosis “in situ” using latex particles2
Current technologies for anti-ENA antibody detection: State-of-the-art of diagnostic immunoassays2
Depletion of CD45RA+ T cells: Advantages and disadvantages of different purification methods2
The commutability of enzyme linked immunosorbent assays for the quantification of serum eosinophil-derived neurotoxin (EDN)2
An optimized non-T cell transfection system based on HEK293FT cells for CD3ζ phosphorylation and ubiquitination2
Expression of recombinant dengue virus type 1 non-structural protein 1 in mammalian cells and preliminary assessment of its suitability to detect human IgG antibodies elicited by viral infection2
Development of automated microfluidic immunoassays for the detection of SARS-CoV-2 antibodies and antigen2
Future perspectives on in-vitro diagnosis of drug allergy by the lymphocyte transformation test2
A method to isolate and cryopreserve cerebrospinal fluid mononuclear cells from external ventricular drains to investigate immunological processes in acute brain injuries2
A multi-colour confocal microscopy method for identifying and enumerating macrophage subtypes and adherent cells in the stromal vascular fraction of human adipose2
Editorial Board2
Evaluation of a point of care lateral flow assay for antibody detection following SARS CoV-2 mRNA vaccine series2
SARS-CoV-2 diagnostics: Towards a more comprehensive approach to routine patient testing2
Hybrid recombinant Omp 22, 25, and 31 immunodominant epitopes can be used for serodiagnosis of brucellosis2
Multiplex immunoassay to measure antibody response to nine HPV vaccine types2
Comparison of real-time quantitative PCR and two digital PCR platforms to detect copy number variation in FCGR3B2
A multi-epitope based vaccine against the surface proteins expressed in cyst and trophozoite stages of parasite Entamoeba histolytica2
High-sensitive photometric microplate assay for tumor necrosis factor-alpha based on Fe@BC nanozyme2
Rhesus macaque Bcl-6/Bcl-xL B cell immortalization: Discovery of HIV-1 neutralizing antibodies from lymph node2
A peptide derived from the N-terminal of NS2A for the preparation of ZIKV NS2A recognition polyclonal antibody2
Development of an ELISA displaying similar reactivity with reduced and oxidized human Thioredoxin-1 (Trx1): The plasma level of Trx1 in early onset psychosis disorders2
Letter to the editor concerning “Development and validation of indirect ELISA for antibody detection against different protein antigens of porcine epidemic diarrhea virus in the colostrum and milk of 2
Methods for studying P2X4 receptor ion channels in immune cells2
Editorial Board2
Development of a long-term, IL7 dependent cell death rescue assay in CD4+ T-cells2
Quantification of the pro-form of human complement component factor D (adipsin)2
Development and validation of a microfluidic multiplex immunoassay for the determination of levels and avidity of serum antibodies to tetanus, diphtheria and pertussis antigens2
Editorial Board2
Cell-type-specific gene expression profile by laser capture microdissection on mirror sections2
Application of the SARS-CoV-2-S1 ACE-2 receptor interaction as the basis of the fully automated assay to detect neutralizing SARS-CoV-2-S1 antibodies in blood samples2
Isolation and culture of rat intestinal mucosal microvascular endothelial cells using immunomagnetic beads2
Leptospirosis detection: correspondence2
Antibody engineering to generate anti-tumor-associated glycoprotein 72 mouse recombinant CC49 IgG with improved solubility, purity, and thermal stability2
Editorial Board2
Flow cytometry as a diagnostic tool in neuroblastoma2
Identification of tumor-associated antigens of lung cancer: SEREX combined with bioinformatics analysis2
ELISpot assay as a diagnostic tool in drug hypersensitivity reactions2
A homogeneous time-resolved fluorometric energy transfer assay for the binding assessment of FcRn with IgG antibodies2
Editorial Board2
A systematic examination of anti-drug antibody titer estimation: Applied recommendations2
Basophil and mast cell activation tests by flow cytometry in immediate drug hypersensitivity: Diagnosis and beyond2
A switchable secrete-and-capture system enables efficient selection of Pichia pastoris clones producing high yields of Fab fragments2
Assessment of mimicking by EBV-CMV immunoglobulin M of anti-HLA antibodies2
Development of a sensitive and stable chemiluminescent immunoassay for detection of birch pollen allergic specific IgE based on recombinant Bet v1 protein2
A flow cytometry-based assay to determine the ability of anti-Streptococcus pyogenes antibodies to mediate monocytic phagocytosis in human sera2
Autotransporter-based surface expression and complementation of split TreA fragments utilized for the detection of antibodies against bovine leukemia virus2
Peptide Hamming Graphs: A network representation of peptides presented through specific HLAs to identify potential epitope clusters2
Development and basic performance verification of a rapid homogeneous bioassay for agonistic antibodies against the thyroid-stimulating hormone receptor2
Antinuclear antibodies (ANAs) detected by indirect immunofluorescence (IIF) method in acute COVID-19 infection; future roadmap for laboratory diagnosis2
Novel IgE crosslinking-induced luciferase expression method using human-rat chimeric IgE receptor-carrying mast cells2
Performance characteristics of the BRAHMS KRYPTOR automated squamous cell carcinoma antigen assay2
Evaluation of commercially available antibodies and fluorescent conotoxins for the detection of surface ganglionic acetylcholine receptor on the neuroblastoma cell line, IMR-32 by flow cytometry2
Nanobody-based microfluidic human Fc assay for preclinical plasma quantification of IgG1/1.1 and IgG1-Fc-conjugates2
Cocaine binding to the Fab fragment of a humanized anti-cocaine mAb quantitated by dye absorption and fluorescence spectroscopy2
Immunocartography: Charting vaccine-driven immunity by applying single cell proteomics to an in vitro human model2
Cross-reactivity of T cell-specific antibodies in the bank vole (Myodes glareolus)2
Editorial Board1
A taxonomy of multiple regression methods for immunologists1
Corrigendum to “A taxonomy of multiple regression methods for immunologists” [Journal of Immunological Methods (2023) pp. 1–6 / Article No. 113506]1
PLGA nanoparticles containing Intimin-Flagellin fusion protein for E. coli O157:H7 nano-vaccine1
Comparative evaluation of Luminex based assays for detection of SARS-CoV-2 antibodies in a transplantation laboratory1
Anti-cytokine autoantibodies and inborn errors of immunity1
The spike-ACE2 binding assay: An in vitro platform for evaluating vaccination efficacy and for screening SARS-CoV-2 inhibitors and neutralizing antibodies1
A cell-based subtractive panning strategy for selection of conformation-specific single-chain variable-fragment (scFv) against dimerization domain of EGFR1
PD-BAT: A novel approach of pooling basophil donors for expansion of commercial laboratory testing of Chronic Spontaneous Urticaria1
Workflow improvement and financial gain after integration of high-throughput sample processing system with flow cytometer in a high-volume pathology laboratory: Results from a prospective comparative 1
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