Journal of Immunological Methods

Article	Citations
Editorial Board	62
Inter-intra instrument comparison and standardization of a 10-color immunophenotyping for B and T cell non-Hodgkin lymphoma diagnosis and monitoring	42
Appropriate immunoassay systems are needed to evaluate various anticentromere antibodies as biomarkers for disease progression in systemic sclerosis	27
Combined RNAscope and immunohistochemistry staining on duodenal paraffin sections as a new tool to reveal cytolytic potential of intraepithelial lymphocytes	22
A FluoroSpot B assay for the detection of IgA and IgG SARS-CoV-2 spike-specific memory B cells: Optimization and qualification for use in COVID-19 vaccine trials	21
Phospho-flow cytometry assays for diagnostic use – A discussion of assay utility and assay development and validation challenges	21
Agreement and error of titration assays	20
Optimized cytotoxicity assay for co-suspended effector and target cells	18
Development of a new automated IL-6 immunoassay	18
Decreased variability in the site-specific results during participation in the External Quality Assurance Program Oversight Laboratory (EQAPOL) proficiency program for IFN-gamma enzyme-linked immunosp	17
Editorial Board	16
Phage display selection of human single domain antibodies towards karilysin, a metalloproteinase and secreted virulence factor from Tannerella forsythia	16
Techniques for evaluating the ATP-gated ion channel P2X7 receptor function in macrophages and microglial cells	15
A sensitive and drug tolerant assay for detecting anti-AAV9 antibodies using affinity capture elution	15
Antibodies against chikungunya virus structural proteins reveal the presence of CHIKV virions in migrasomes	15
Comparability study of monocyte derived dendritic cells, primary monocytes, and THP1 cells for innate immune responses	14
Smartphone based lateral flow immunoassay quantifications	13
Development and validation of dengue virus envelope protein domain III IgG antibody enzyme-linked immunosorbent assay	13
Candidate antibody reference reagents for Chlamydia trachomatis serology	13
Development of optimized cytotoxicity assays for assessing the antitumor potential of CAR-T cells	13
Challenges for complement functional assays in the clinical laboratory: From test validation to clinical interpretation	12
Editorial Board	12
Dried blood spots are a valid alternative to venipuncture for COVID-19 antibody testing	11
Quantitative flow cytometry using quantitative streptavidin-protein G-biotin beads (qBeads)	11
Flow cytometry reveals constant lymphocyte proportions after long-term cryopreservation of whole blood in TransFix® cell stabilization reagent	11

Divalent cations and SERPIN gene expression modulate horseshoe crab coagulation under LPS induction	11
Complete primer set for amplification and expression of full-length recombinant human monoclonal antibodies from single human B cells	11
Rapid, sensitive and cost-effective determination of immune checkpoint inhibitor activity using a magnetic bead-based binding assay	11
Development of an ELISA strategy for the serological diagnosis of farmer's lung disease	11
Development of automated microfluidic immunoassays for the detection of SARS-CoV-2 antibodies and antigen	10
An unexpected bioanalytical challenge caused by positive control antibodies in a clinical immunogenicity assay – A simple solution and broadly applicable recommendations	10
Target tolerance improvement of the immunogenicity assay developed for analysing samples from clinical trials of RGB-14, a proposed biosimilar to Prolia/Xgeva	10
Current technologies for anti-ENA antibody detection: State-of-the-art of diagnostic immunoassays	10
Optimisation of SARS-CoV-2 peptide stimulation and measurement of cytokine output by intracellular flow cytometry and bio-plex analysis	10
Humoral and cellular response of two different vaccines against SARS-CoV-2 in a group of healthcare workers: Comment	9
Performance characteristics of the BRAHMS KRYPTOR automated squamous cell carcinoma antigen assay	9
Preparation and specific identification of monoclonal antibodies targeting the VP2 structural protein of minute virus of canines	9
Humoral and cellular response of two different vaccines against SARS-CoV-2 in a group of healthcare workers: An observational study	9
Leptospirosis detection: correspondence	9
A systematic examination of anti-drug antibody titer estimation: Applied recommendations	9
Evaluation of self-collected dried blood spots for detection of SARS-CoV-2 nucleocapsid antibodies shows low sensitivity	9
Cocaine binding to the Fab fragment of a humanized anti-cocaine mAb quantitated by dye absorption and fluorescence spectroscopy	9
Editorial Board	9
Cross-reactivity of T cell-specific antibodies in the bank vole (Myodes glareolus)	9
Comparison of real-time quantitative PCR and two digital PCR platforms to detect copy number variation in FCGR3B	9
Evaluation and correlation between SARS-CoV-2 neutralizing and binding antibodies in convalescent and vaccinated subjects	8
Inducing and regulating human naive CD4+ T cell proliferation by different antigen presenting cells	8
A 33-color panel of phenotypic analysis of murine organ specific immune cells	8
Murine model of steroid-resistant neutrophilic bronchial asthma as an attempt to simulate human pathology	7
Indirect ELISAs with sucrose subcellular fractions of Neospora caninum as antigens for diagnosis of neosporosis in cattle	7
High-performance immune diagnosis of tuberculosis: Use of phage display and synthetic peptide in an optimized experimental design	7
Magnetic CAR T cell purification using an anti-G4S linker antibody	7
A comprehensive method for the phenotypical and functional characterization of recalled human memory B and T cells specific to vaccine antigens	7
Effect of storage time on peripheral blood mononuclear cell isolation from blood collected in vacutainer CPT™ tubes	7
Long term investigation of formulation buffers to mitigate stability issues of conjugated critical reagents	7
NADPH oxidase subunit p22 : A marker of oxidase-dependent oxidative stress and target for stress suppression in nonphagocytic cells	7
A cell-based subtractive panning strategy for selection of conformation-specific single-chain variable-fragment (scFv) against dimerization domain of EGFR	7
Fragments of bombinakinin M exist in lipopolysaccharide-stimulated skin secretions of Bombina maxima and show lipopolysaccharide-neutralizing activity	7
Optimization of blood sampling time for isolation of antigen binding single-domain antibodies from immunized llamas	7
An optimized flow cytometry panel for classifying macrophage polarization	7
A novel strategy to investigate the factors regulating the Treg to Tfr transition during acute viral infection	7
Comparative evaluation of Luminex based assays for detection of SARS-CoV-2 antibodies in a transplantation laboratory	7
Identifying therapeutic monoclonal antibodies using target protein collision electrophoresis reflex assay to separate the wheat from the chaff	7
Preliminary field evaluation of indirect ELISA test using the recombinant antigen rLicNTPDase-2 for serodiagnosis of canine leishmaniasis in Colombia	7
Development of sandwich enzyme-linked immunosorbent assays quantifying mouse urinary megalin, a novel proximal tubular biomarker	7
Monoclonal antibody on-rate constant determined from time-course data of ligand binding by capture ELISA: Evaluation of eight data analysis methods	7
Determination of echinococcosis IgG antibodies using magnetic bead-based chemiluminescence immunoassay	7
Development and assessment of a new bioassay for accurate quantification of Type I interferons induced by bovine respiratory viruses	7
1,25-dihydroxyvitamin D3 augments low-dose PMA-based monocyte-to-macrophage differentiation in THP-1 cells	6
Validation of dried blood spot sampling for detecting SARS-CoV-2 antibodies and total immunoglobulins in a large cohort of asymptomatic young adults	6
Isolation and expansion of murine γδ T cells from mouse splenocytes	6
VaxArray immunoassay for the multiplexed quantification of poliovirus D-antigen	6
The Fas-mediated apoptosis assay: From concept to clinical application	6
True volumetric counting of CD34+ cells using flow cytometry	6
Development of a high-throughput image cytometric screening method as a research tool for immunophenotypic characterization of patient samples from clinical studies	6

A novel panel of peptides with diagnostic potential for serological identification of Borrelia burgdorferi sensu stricto, B. garinii and B. afzelii in human sera	6
Production and characterization of novel monoclonal antibodies against outer membrane protein A (OmpA) of Acinetobacter baumannii	6
Antisera against flagellin A or B inhibits Pseudomonas aeruginosa motility as measured by novel video microscopy assay	6
Development and validation of an in vitro titrimetric method for determination of classical swine fever viruses in PK-15 cells	6
Editorial Board	6
Evaluation of refractometry methods for estimating passive immunity status in neonatal foals	6
Editorial Board	6
Development and optimization of a diluted whole blood ELISpot assay to test immune function	6
Standardization, validation, and comparative evaluation of a faster and high-performance test for quantification of yellow fever neutralizing antibodies	6
In silico designed Staphylococcus aureus B-cell multi-epitope vaccine did not elicit antibodies against target antigens suggesting multi-domain approach	6
Overcoming cross-reactivity of antibodies against human lactate dehydrogenase	6
Nephelometry vs. Immunoturbidimetry assay: Analytical performance on IgG subclasses	6
Strategies to determine positive anti-SARS-CoV-2 memory T lymphocyte response during the evolution of an epidemic	6
An enzyme-free method for isolating testicular macrophages from rodent models	5
Performance and validation of an adaptable multiplex assay for detection of serologic response to SARS-CoV-2 infection or vaccination	5
Development and characterization of an ETV1 rabbit monoclonal antibody for the immunohistochemical detection of ETV1 expression in cancer tissue specimens	5
Miniaturized CAR knocked onto CD3ε extends TCR function with CAR specificity under control of endogenous TCR signaling cascade	5
Establishment of growth stimulating gene 2 protein time-resolved fluorescence immunoassay and its application in sepsis	5
Editorial Board	5
Detection of drug-specific immunoglobulin E (IgE) and acute mediator release for the diagnosis of immediate drug hypersensitivity reactions	5
Clinical utility of the lymphocyte proliferation assay, an in vitro functional readout of the adaptive immune response	5
Publisher's note: Challenges for complement functional assays in the clinical laboratory: From test validation to clinical interpretation	5
Study of avidity-ELISA: Comparison of chaotropic agents, incubation temperature and affinity maturation after meningococcal immunization	5
A note of caution for using calmodulin antibodies	5
Editorial Board	5
Rapid, sensitive, and specific lateral-flow immunochromatographic point-of-care device for detection of varicella-zoster virus immunoglobulin G antibodies in fingerstick blood	5
Evaluation of cell-mediated immunity of E.coli nanovaccines in chickens	5
30-color full spectrum flow cytometry panel for deep immunophenotyping of T cell subsets in murine tumor tissue	5
Sensitive amplified luminescent proximity homogeneous assay for the quantitative detection of CA242	5
Development of a Bak gene based standard curve for absolute quantification of BK virus in real time quantitative PCR assay and noninvasive diagnosis of BK virus nephropathy in kidney allograft recipie	5
Methodological approaches to optimize multiplex oral fluid SARS-CoV-2 IgG assay performance and correlation with serologic and neutralizing antibody responses	5
Editorial Board	5
Development of a proficiency testing program for HPV serology assays used to evaluate antibody responses in vaccine trials	5
Editorial Board	5
Plaque reduction neutralization test for smallpox vaccines: Laboratory optimization and validation method for immunogenicity assessment	5
Validation of the performance and suitability of a new class of FBS optimized for use in single-cell functional assays	5
Highly effective strategy for isolation of mononuclear cells from frozen cord blood	4
Hybrid recombinant Omp 22, 25, and 31 immunodominant epitopes can be used for serodiagnosis of brucellosis	4
Flow cytometry for B-cell subset analysis in immunodeficiencies	4
Comparison of two anti-histones antibodies commercial assays in an immunology laboratory and systemic lupus erythematosus	4
Diagnosis of septic shock by serum measurement of human neutrophil lipocalin by a rapid homogeneous assay	4
Evaluation of commercially available antibodies and fluorescent conotoxins for the detection of surface ganglionic acetylcholine receptor on the neuroblastoma cell line, IMR-32 by flow cytometry	4
A switchable secrete-and-capture system enables efficient selection of Pichia pastoris clones producing high yields of Fab fragments	4
Basophil activation test; User's manual	4
Novel in vitro invariant natural killer T cell functional assays	4
Complement function and activation in human serum and plasma collected in different blood collection tubes	4
A universal urinary cell gene signature of acute rejection in kidney allografts	4
The mouse monoclonal antibody 4E3 is specific for the G1m17 allotype of human IgG1	4
Artificial intelligence and deep learning to map immune cell types in inflamed human tissue	4
Tumor nano-lysate activates dendritic cells to evoke a preventative immune response	4
Generation of an alpaca serum that induces immune-mediated crescentic glomerulonephritis in mice	4
Letter to the editor concerning “Development and validation of indirect ELISA for antibody detection against different protein antigens of porcine epidemic diarrhea virus in the colostrum and milk of	4
Differences in mouse strains determine the outcome of Der p 2 allergy induction protocols	4
Chemiluminescent detection of platelet derived growth factor-BB based on sandwich label-free aptasensor and biotin–streptavidin strategy	4
Highly sensitive detection of carcinoembryonic antigen via an electrochemical platform fabricated by CCLP- gold nanocomposite	4
Multiplex ACE2-RBD binding inhibition assay: An integrated tool for assessing neutralizing antibodies to SARS-CoV-2 variants and protection against breakthrough infections	4
Multiplex immunoassay to measure antibody response to nine HPV vaccine types	4
PD-BAT: A novel approach of pooling basophil donors for expansion of commercial laboratory testing of Chronic Spontaneous Urticaria	4
Development of an enteric pathogen multiplex immunoassay to measure antibody responses in blood and saliva for integrated serology applications	4
Detection of SARS-CoV-2 peptide-specific antibodies in Syrian hamster serum by ELISA	4
Cell-type-specific gene expression profile by laser capture microdissection on mirror sections	4
Dissociation of murine oral mucosal tissues for single cell applications	4
Isolation of anti-Ancylostoma-secreted protein 5 (ASP5) antibody from a naïve antibody phage library	4
Optimized flow cytometry protocol for dihydrorhodamine 123-based detection of reactive oxygen species in leukocyte subpopulations in whole blood	4
A flow cytometry-based assay to determine the ability of anti-Streptococcus pyogenes antibodies to mediate monocytic phagocytosis in human sera	4
Qualification of ELISA and neutralization methodologies to measure SARS-CoV-2 humoral immunity using human clinical samples	4
A taxonomy of multiple regression methods for immunologists	4
COVID-19 in patients with B cell immune deficiency	4
A peptide derived from the N-terminal of NS2A for the preparation of ZIKV NS2A recognition polyclonal antibody	4
In silico engineering a CD80 variant with increased affinity to CTLA-4 and decreased affinity to CD28 for optimized cancer immunotherapy	3
A standarized protocol for evaluation of large extracellular vesicles using the attune™ NXT system	3
Expression of bluetongue virus full-length VP7 protein in insect cells and its diagnostic utility for detection of antibodies to the virus infection	3
Repeatability of QuantiFERON-TB gold plus testing utilizing microparticle chemiluminescence	3
A dried blood spot test for diagnosis of autoimmune pulmonary alveolar proteinosis	3
Editorial Board	3
Identification of monoclonal antibodies from naive antibody phage-display libraries for protein detection in formalin-fixed paraffin-embedded tissues	3
SARS-CoV-2 spike protein expression as an identification in quality control testing for Adenovector based COVID-19 vaccine	3
Skeletal muscle macrophage ablation in mice	3
Application of microphysiologic system to assess neutrophil extracellular trap in xenotransplantation	3
Optimization of IgG1 immune complexes to stimulate cytokine production in innate cells	3

Description of a non-competitive ELISA based on time course analysis of ligand binding at saturation, and a direct method for calculating the affinity of monoclonal antibodies	3
Editorial Board	3
Clinical and analytical evaluation of the Abbott AdviseDx quantitative SARS-CoV-2 IgG assay and comparison with two other serological tests	3
Editorial Board	3
Reference intervals for functional lymphocyte proliferation studies using 3H thymidine uptake in adults	3
Optimized flow cytometry panel for the detection and analysis of human tumor-induced memory-like NK cells	3
Theoretical and practical considerations for validating antigen-specific B cell ImmunoSpot assays	3
Editorial Board	3
Application of a caprylate/chromatography purification process for production of a high potency rabies immune globulin from pooled human plasma	3
Development of a rapid lateral flow immunochromatography assay for the detection of group-specific antibodies against VP7 protein of bluetongue virus in multiple species	3
ELISA protein detector (EPD): A Python-based ELISA tool for accurate low-level protein quantification	3
Discrepancies between two total IgE assays and difference in reference intervals in healthy adults	3
A high-throughput assay using dysfunctional T cells for phenotypic screening immune checkpoint modulators	3
Development of a novel anti-PEG antibody assay enabling investigation of potential immunogenicity triggered by the PEG moiety of biotherapeutics	3
A flow cytometry-based proliferation assay for clinical evaluation of T-cell memory against SARS-CoV-2	3
Editorial Board	3
Generation of Q-bead against bone Gla protein with simplified preparation steps	3
A comprehensive multiparameter flow cytometry panel for immune profiling and functional studies of frozen tissue, bone marrow, and spleen	3
Novel bridge multi-species ELISA for detection of SARS-CoV-2 antibodies	3
A light-initiated chemiluminescent assay for the detection of children's milk protein-specific IgE with excellent ability to avoid interference of specific IgG	3
Platelets isolation and ectonucleotidase assay: Revealing functional aspects of the communication between the vasculature and the immune system	3
Evaluation of multiple immunoassay formats for detection of anti-drug antibodies to zinpentraxin alfa	3
Editorial Board	3
Chimeric protein A/G conjugate based lateral flow assay for the rapid detection of brucellosis in multiple animal species	3
A rapid and high-throughput T cell immunophenotyping assay for cellular therapy bioprocess using the Cellaca® PLX image cytometer	3
A novel HRM-based gap-qRT-PCR for identification and quantitation of the vaccine and field strain(s) of lumpy skin disease virus	3
Preparation and characterization of a neutralizing murine monoclonal antibody against tetanus toxin	3
A flexible gradient lateral flow immunochromatographic assay for qualitative, semi-quantitative, and quantitative determination of serum amyloid A	3
Immunoinformatics-guided designing of epitope-based subunit vaccine from Pilus assembly protein of Acinetobacter baumannii bacteria	3
Fit-for-purpose validation of a drug-tolerant immunogenicity assay for a human mAb drug in animal safety studies	3
Preparation of substrates for microarray protein chips with different ending functional groups	3
Impact of bridge heterology on functional parameters of ELISA for 17α-methyltestosterone	3
Human monoclonal antibody cloning and expression with overlap extension PCR and short DNA fragments	2
Optimizing the method for expressing human monoclonal antibodies from a single peripheral blood cell from vaccinated donors	2
Development and laboratory validation of an electrochemiluminescence ELISA technique for measuring infliximab concentrations and anti-drug antibodies	2
Whole blood based point-of-care assay for the detection of anti-pertussis toxin IgG antibodies	2
Validation for soluble C5b-9 detection and comparative analysis of three quantification methods	2
Methyl ester and aromatic ether modification of mitragynine for generation of mitragynine-specific polyclonal antibodies	2
Simultaneous determination of inflammatory factors SAA and LTF based on stable element labeling and inductively coupled plasma mass spectrometry to aid in the diagnosis of infection	2
Comparison of methods to isolate peripheral blood mononuclear cells from cattle blood	2
Fresh versus frozen PMBC using the SARS CoV-2 T-SPOT.COVID test. Which works best?	2
Novel IgE crosslinking-induced luciferase expression method using human-rat chimeric IgE receptor-carrying mast cells	2
An eight-plex immunoassay for Group A streptococcus serology and vaccine development	2
Multiplex agglutination-PCR (ADAP) autoantibody assays compared to radiobinding autoantibodies in type 1 diabetes and celiac disease	2
Proficiency tests to evaluate the impact on assay outcomes of harmonized influenza-specific Intracellular Cytokine Staining (ICS) and IFN-ɣ Enzyme-Linked ImmunoSpot (ELISpot) protocols	2
Development of an indirect ELISA for detection of the adaptive immune response of black carp (Mylopharyngodon piceus)	2
Editorial Board	2
Editorial Board	2
Development and evaluation of a low cost IgG ELISA test based in RBD protein for COVID-19	2
Anti-cytokine autoantibodies and inborn errors of immunity	2
Advanced hybridoma technology for selective production of high-affinity monoclonal antibodies through B-cell receptors	2
A real-time antibody-dependent cellular phagocytosis assay by live cell imaging	2
Development and evaluation of a multi-antigen serological assay for the intra-vitam diagnosis of Tuberculosis caused by Mycobacterium bovis in pigs	2
Antibody engineering to generate anti-tumor-associated glycoprotein 72 mouse recombinant CC49 IgG with improved solubility, purity, and thermal stability	2
Antibody detection by agglutination-PCR (ADAP) assays for the analysis of tissue transglutaminase autoantibodies in celiac disease	2
Identification of the linear Fc-binding epitope on the bovine IgG1 Fc receptor of (boFcγRI) using synthetic peptides	2
Flow cytometry as a diagnostic tool in neuroblastoma	2
Stabilization of leukocytes from cerebrospinal fluid for central immunophenotypic evaluation in multicenter clinical trials	2
Sources of variability in Luminex bead-based cytokine assays: Evidence from twelve years of multi-site proficiency testing	2
Application of the SARS-CoV-2-S1 ACE-2 receptor interaction as the basis of the fully automated assay to detect neutralizing SARS-CoV-2-S1 antibodies in blood samples	2
A method to isolate and cryopreserve cerebrospinal fluid mononuclear cells from external ventricular drains to investigate immunological processes in acute brain injuries	2
Indirect ELISA diagnostic based on recombinant 3AB3 nonstructural protein of foot-and-mouth disease virus for use in porcine serology	2
The crux of C1-INH testing in everyday lab work	2
A novel immuno-molecular strategy for the detection of Streptococcus pneumoniae serotypes in human cerebrospinal and middle ear fluids	2
Obtaining a high titer of polyclonal antibodies from rats to the SARS-CoV-2 nucleocapsid protein and its N- and C-terminal domains for diagnostic test development	2
Peptide Hamming Graphs: A network representation of peptides presented through specific HLAs to identify potential epitope clusters	2
Development of a concise and reliable method for quantifying the antibody loaded onto lipid nanoparticles modified with Herceptin	2
Nanobody-based microfluidic human Fc assay for preclinical plasma quantification of IgG1/1.1 and IgG1-Fc-conjugates	2
Editorial Board	2
Optimization of lateral flow assay for Canine morbillivirus detection and the application of the strip as sample substitute	2
Corrigendum to “A taxonomy of multiple regression methods for immunologists” [Journal of Immunological Methods (2023) pp. 1–6 / Article No. 113506]	2
Rapid identification of tumor-reactive T-cell receptors by RNA preamplification-based single-cell sequencing	2
Isolation and culture of rat intestinal mucosal microvascular endothelial cells using immunomagnetic beads	2
Evaluation of a point of care lateral flow assay for antibody detection following SARS CoV-2 mRNA vaccine series	2
Editorial Board	2
Development of multiplexed flow based assay for simultaneous identification and estimation of meningococcal capsular polysaccharide serogroups A, C, W, Y and X for conjugate vaccine manufacturing	2
Development and performance evaluation of a novel, fully automated, high-throughput GAD65 autoantibody assay on the Phadia platform	2
Editorial Board	2
The role of gamma globulin, complement component 1q, factor B, properdin, body temperature, C-reactive protein and serum amyloid alpha to the activity and the function of the human complement system a	2
Assessment of an in-house IgG ELISA targeting SARS-CoV-2 RBD: Applications in infected and vaccinated individuals	2
Comparison of mixed modeling regression methods for the assessment of longitudinal CyTOF® data	2
Development and characterization of a latex turbidimetric immunoassay using rabbit anti-CRP single-chain Fv antibodies	2
Differential composition and yield of leukocytes isolated from various blood component leukoreduction filters	2
Editorial Board	2
A homogeneous time-resolved fluorometric energy transfer assay for the binding assessment of FcRn with IgG antibodies	2
Editorial Board	2
Assessment of mimicking by EBV-CMV immunoglobulin M of anti-HLA antibodies	2
Evaluation of autofluorescence quenching techniques on formalin- fixed chicken tissues	2
Retention of E-selectin functionalized liposome fanny packs on Jurkat cells following invasion through collagen	1
An in-silico-designed multiepitope vaccine candidate can efficiently protect mice against pathogenic species of Shigella	1
“Common variable immunodeficiency: Challenges for diagnosis”	1
Development of a defibrinated human blood hemolysis assay for rapid testing of hemolytic activity compared to computational prediction	1
Editorial Board	1
Development of a novel histidine-rich glycoprotein measurement system as a biomarker for sepsis	1
Stability of anti-drug antibodies in human serum samples	1