Journal of Immunological Methods

Papers
(The median citation count of Journal of Immunological Methods is 1. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-06-01 to 2025-06-01.)
ArticleCitations
Immunoglobulin isotype compositions of ABO specific antibodies are dependent on the individual patient blood group and blood group specificity: Results from a healthy donor cohort61
Editorial Board58
Inter-intra instrument comparison and standardization of a 10-color immunophenotyping for B and T cell non-Hodgkin lymphoma diagnosis and monitoring34
Appropriate immunoassay systems are needed to evaluate various anticentromere antibodies as biomarkers for disease progression in systemic sclerosis30
Combined RNAscope and immunohistochemistry staining on duodenal paraffin sections as a new tool to reveal cytolytic potential of intraepithelial lymphocytes26
Phospho-flow cytometry assays for diagnostic use – A discussion of assay utility and assay development and validation challenges25
Establishment of a homogeneous immunoassay-light-initiated chemiluminescence assay for detecting anti-Müllerian hormone in human serum24
A FluoroSpot B assay for the detection of IgA and IgG SARS-CoV-2 spike-specific memory B cells: Optimization and qualification for use in COVID-19 vaccine trials21
Agreement and error of titration assays20
Optimized cytotoxicity assay for co-suspended effector and target cells19
Development of a new automated IL-6 immunoassay19
Decreased variability in the site-specific results during participation in the External Quality Assurance Program Oversight Laboratory (EQAPOL) proficiency program for IFN-gamma enzyme-linked immunosp18
A sensitive and drug tolerant assay for detecting anti-AAV9 antibodies using affinity capture elution17
Editorial Board16
Antibodies against chikungunya virus structural proteins reveal the presence of CHIKV virions in migrasomes16
Phage display selection of human single domain antibodies towards karilysin, a metalloproteinase and secreted virulence factor from Tannerella forsythia15
Development and validation of dengue virus envelope protein domain III IgG antibody enzyme-linked immunosorbent assay15
Candidate antibody reference reagents for Chlamydia trachomatis serology15
Challenges for complement functional assays in the clinical laboratory: From test validation to clinical interpretation15
Development of optimized cytotoxicity assays for assessing the antitumor potential of CAR-T cells15
Techniques for evaluating the ATP-gated ion channel P2X7 receptor function in macrophages and microglial cells14
Smartphone based lateral flow immunoassay quantifications13
Rapid, sensitive and cost-effective determination of immune checkpoint inhibitor activity using a magnetic bead-based binding assay13
Comparability study of monocyte derived dendritic cells, primary monocytes, and THP1 cells for innate immune responses13
Editorial Board13
Dried blood spots are a valid alternative to venipuncture for COVID-19 antibody testing12
Flow cytometry reveals constant lymphocyte proportions after long-term cryopreservation of whole blood in TransFix® cell stabilization reagent12
Validation of a combined ELISA to detect IgG, IgA and IgM antibody responses to SARS-CoV-2 in mild or moderate non-hospitalised patients12
Development and qualification of a fast, high-throughput and robust imaging-based neutralization assay for respiratory syncytial virus11
Humoral and cellular response of two different vaccines against SARS-CoV-2 in a group of healthcare workers: An observational study11
A 33-color panel of phenotypic analysis of murine organ specific immune cells11
Divalent cations and SERPIN gene expression modulate horseshoe crab coagulation under LPS induction11
Quantitative flow cytometry using quantitative streptavidin-protein G-biotin beads (qBeads)11
Current technologies for anti-ENA antibody detection: State-of-the-art of diagnostic immunoassays11
Complete primer set for amplification and expression of full-length recombinant human monoclonal antibodies from single human B cells11
Immunoinformatics and Pepscan strategies on the path of a peptide-based serological diagnosis of COVID1911
Leptospirosis detection: correspondence11
A systematic examination of anti-drug antibody titer estimation: Applied recommendations10
Editorial Board10
Cross-reactivity of T cell-specific antibodies in the bank vole (Myodes glareolus)10
Performance characteristics of the BRAHMS KRYPTOR automated squamous cell carcinoma antigen assay10
Humoral and cellular response of two different vaccines against SARS-CoV-2 in a group of healthcare workers: Comment10
Optimisation of SARS-CoV-2 peptide stimulation and measurement of cytokine output by intracellular flow cytometry and bio-plex analysis9
Evaluation of self-collected dried blood spots for detection of SARS-CoV-2 nucleocapsid antibodies shows low sensitivity9
Autotransporter-based surface expression and complementation of split TreA fragments utilized for the detection of antibodies against bovine leukemia virus9
Development of automated microfluidic immunoassays for the detection of SARS-CoV-2 antibodies and antigen9
Preparation and specific identification of monoclonal antibodies targeting the VP2 structural protein of minute virus of canines9
Target tolerance improvement of the immunogenicity assay developed for analysing samples from clinical trials of RGB-14, a proposed biosimilar to Prolia/Xgeva9
Comparison of real-time quantitative PCR and two digital PCR platforms to detect copy number variation in FCGR3B9
Cocaine binding to the Fab fragment of a humanized anti-cocaine mAb quantitated by dye absorption and fluorescence spectroscopy9
Determination of echinococcosis IgG antibodies using magnetic bead-based chemiluminescence immunoassay8
Protective efficacy of Hla-MntC-SACOL0723 fusion protein adjuvanted in alum and MPL against Staphylococcus aureus sepsis infection in mice8
Magnetic CAR T cell purification using an anti-G4S linker antibody8
Evaluation and correlation between SARS-CoV-2 neutralizing and binding antibodies in convalescent and vaccinated subjects8
Inducing and regulating human naive CD4+ T cell proliferation by different antigen presenting cells8
Fragments of bombinakinin M exist in lipopolysaccharide-stimulated skin secretions of Bombina maxima and show lipopolysaccharide-neutralizing activity8
High-performance immune diagnosis of tuberculosis: Use of phage display and synthetic peptide in an optimized experimental design8
Identifying therapeutic monoclonal antibodies using target protein collision electrophoresis reflex assay to separate the wheat from the chaff8
Development of sandwich enzyme-linked immunosorbent assays quantifying mouse urinary megalin, a novel proximal tubular biomarker7
NADPH oxidase subunit p22 : A marker of oxidase-dependent oxidative stress and target for stress suppression in nonphagocytic cells7
Diagnostic and analytical performance evaluation of ten commercial assays for detecting SARS-CoV-2 humoral immune response7
Monoclonal antibody on-rate constant determined from time-course data of ligand binding by capture ELISA: Evaluation of eight data analysis methods7
Murine model of steroid-resistant neutrophilic bronchial asthma as an attempt to simulate human pathology7
An optimized flow cytometry panel for classifying macrophage polarization7
Preliminary field evaluation of indirect ELISA test using the recombinant antigen rLicNTPDase-2 for serodiagnosis of canine leishmaniasis in Colombia7
A comprehensive method for the phenotypical and functional characterization of recalled human memory B and T cells specific to vaccine antigens7
Optimization of blood sampling time for isolation of antigen binding single-domain antibodies from immunized llamas7
A novel strategy to investigate the factors regulating the Treg to Tfr transition during acute viral infection6
Indirect ELISAs with sucrose subcellular fractions of Neospora caninum as antigens for diagnosis of neosporosis in cattle6
Performance of a commercially available multiplex platform in the assessment of circulating cytokines and chemokines in patients with rheumatoid arthritis and osteoarthritis6
1,25-dihydroxyvitamin D3 augments low-dose PMA-based monocyte-to-macrophage differentiation in THP-1 cells6
Production and characterization of novel monoclonal antibodies against outer membrane protein A (OmpA) of Acinetobacter baumannii6
Development and validation of an in vitro titrimetric method for determination of classical swine fever viruses in PK-15 cells6
Editorial Board6
A novel panel of peptides with diagnostic potential for serological identification of Borrelia burgdorferi sensu stricto, B. garinii and B. afzelii in human sera6
Long term investigation of formulation buffers to mitigate stability issues of conjugated critical reagents6
Comparative evaluation of Luminex based assays for detection of SARS-CoV-2 antibodies in a transplantation laboratory6
Development of a high-throughput image cytometric screening method as a research tool for immunophenotypic characterization of patient samples from clinical studies6
Isolation and expansion of murine γδ T cells from mouse splenocytes6
Standardization, validation, and comparative evaluation of a faster and high-performance test for quantification of yellow fever neutralizing antibodies6
True volumetric counting of CD34+ cells using flow cytometry6
Antisera against flagellin A or B inhibits Pseudomonas aeruginosa motility as measured by novel video microscopy assay6
Development and optimization of a diluted whole blood ELISpot assay to test immune function6
Effect of storage time on peripheral blood mononuclear cell isolation from blood collected in vacutainer CPT™ tubes6
Development and assessment of a new bioassay for accurate quantification of Type I interferons induced by bovine respiratory viruses6
Strategies to determine positive anti-SARS-CoV-2 memory T lymphocyte response during the evolution of an epidemic6
Nephelometry vs. Immunoturbidimetry assay: Analytical performance on IgG subclasses6
In silico designed Staphylococcus aureus B-cell multi-epitope vaccine did not elicit antibodies against target antigens suggesting multi-domain approach6
A cell-based subtractive panning strategy for selection of conformation-specific single-chain variable-fragment (scFv) against dimerization domain of EGFR6
VaxArray immunoassay for the multiplexed quantification of poliovirus D-antigen6
Quantification of trivalent non-replicating rotavirus vaccine antigens in the presence of aluminum adjuvant6
Study of avidity-ELISA: Comparison of chaotropic agents, incubation temperature and affinity maturation after meningococcal immunization5
Development of a Bak gene based standard curve for absolute quantification of BK virus in real time quantitative PCR assay and noninvasive diagnosis of BK virus nephropathy in kidney allograft recipie5
Publisher's note: Challenges for complement functional assays in the clinical laboratory: From test validation to clinical interpretation5
A note of caution for using calmodulin antibodies5
Editorial Board5
Artificial intelligence and deep learning to map immune cell types in inflamed human tissue5
Evaluation of cell-mediated immunity of E.coli nanovaccines in chickens5
Construction of a full-length antibody phage display vector5
Clinical utility of the lymphocyte proliferation assay, an in vitro functional readout of the adaptive immune response5
Miniaturized CAR knocked onto CD3ε extends TCR function with CAR specificity under control of endogenous TCR signaling cascade5
Development of a proficiency testing program for HPV serology assays used to evaluate antibody responses in vaccine trials5
Editorial Board5
Validation of dried blood spot sampling for detecting SARS-CoV-2 antibodies and total immunoglobulins in a large cohort of asymptomatic young adults5
Overcoming cross-reactivity of antibodies against human lactate dehydrogenase5
Editorial Board5
Detection of drug-specific immunoglobulin E (IgE) and acute mediator release for the diagnosis of immediate drug hypersensitivity reactions5
Editorial Board5
Sensitive amplified luminescent proximity homogeneous assay for the quantitative detection of CA2425
Optimisation and validation of a new method for antibody dependent cellular phagocytosis in hepatitis C virus infection5
Performance and validation of an adaptable multiplex assay for detection of serologic response to SARS-CoV-2 infection or vaccination5
Development and characterization of an ETV1 rabbit monoclonal antibody for the immunohistochemical detection of ETV1 expression in cancer tissue specimens5
The Fas-mediated apoptosis assay: From concept to clinical application5
Evaluation of refractometry methods for estimating passive immunity status in neonatal foals5
An enzyme-free method for isolating testicular macrophages from rodent models5
Editorial Board5
30-color full spectrum flow cytometry panel for deep immunophenotyping of T cell subsets in murine tumor tissue5
Validation of the performance and suitability of a new class of FBS optimized for use in single-cell functional assays5
Establishment of growth stimulating gene 2 protein time-resolved fluorescence immunoassay and its application in sepsis5
Methodological approaches to optimize multiplex oral fluid SARS-CoV-2 IgG assay performance and correlation with serologic and neutralizing antibody responses5
Development of a non-biased, high-throughput ELISA for the rapid evaluation of immunogenicity and cross-reactivity4
Isolation of anti-Ancylostoma-secreted protein 5 (ASP5) antibody from a naïve antibody phage library4
Dissociation of murine oral mucosal tissues for single cell applications4
The C-terminus of Brucella abortus MviN induces humoral and cell mediated immune responses in BALB/c mice that protects against the virulent Brucella 544 challenge4
A peptide derived from the N-terminal of NS2A for the preparation of ZIKV NS2A recognition polyclonal antibody4
A switchable secrete-and-capture system enables efficient selection of Pichia pastoris clones producing high yields of Fab fragments4
The mouse monoclonal antibody 4E3 is specific for the G1m17 allotype of human IgG14
Cell-type-specific gene expression profile by laser capture microdissection on mirror sections4
Comparison of two anti-histones antibodies commercial assays in an immunology laboratory and systemic lupus erythematosus4
Generation of an alpaca serum that induces immune-mediated crescentic glomerulonephritis in mice4
Plaque reduction neutralization test for smallpox vaccines: Laboratory optimization and validation method for immunogenicity assessment4
Highly effective strategy for isolation of mononuclear cells from frozen cord blood4
Complement function and activation in human serum and plasma collected in different blood collection tubes4
Evaluation of commercially available antibodies and fluorescent conotoxins for the detection of surface ganglionic acetylcholine receptor on the neuroblastoma cell line, IMR-32 by flow cytometry4
A universal urinary cell gene signature of acute rejection in kidney allografts4
Detection of SARS-CoV-2 peptide-specific antibodies in Syrian hamster serum by ELISA4
Optimized flow cytometry protocol for dihydrorhodamine 123-based detection of reactive oxygen species in leukocyte subpopulations in whole blood4
Tumor nano-lysate activates dendritic cells to evoke a preventative immune response4
Diagnosis of septic shock by serum measurement of human neutrophil lipocalin by a rapid homogeneous assay4
Flow cytometry for B-cell subset analysis in immunodeficiencies4
Rapid, sensitive, and specific lateral-flow immunochromatographic point-of-care device for detection of varicella-zoster virus immunoglobulin G antibodies in fingerstick blood4
A flow cytometry-based assay to determine the ability of anti-Streptococcus pyogenes antibodies to mediate monocytic phagocytosis in human sera4
Letter to the editor concerning “Development and validation of indirect ELISA for antibody detection against different protein antigens of porcine epidemic diarrhea virus in the colostrum and milk of 4
Hybrid recombinant Omp 22, 25, and 31 immunodominant epitopes can be used for serodiagnosis of brucellosis4
Novel bridge multi-species ELISA for detection of SARS-CoV-2 antibodies3
A comprehensive multiparameter flow cytometry panel for immune profiling and functional studies of frozen tissue, bone marrow, and spleen3
Culturing cells with mast cell phenotype and function: Comparison of peripheral blood and bone marrow as a source.3
Multiplex ACE2-RBD binding inhibition assay: An integrated tool for assessing neutralizing antibodies to SARS-CoV-2 variants and protection against breakthrough infections3
Highly sensitive detection of carcinoembryonic antigen via an electrochemical platform fabricated by CCLP- gold nanocomposite3
Optimized flow cytometry panel for the detection and analysis of human tumor-induced memory-like NK cells3
ELISpot assay as a diagnostic tool in drug hypersensitivity reactions3
COVID-19 in patients with B cell immune deficiency3
Editorial Board3
Application of a caprylate/chromatography purification process for production of a high potency rabies immune globulin from pooled human plasma3
Development of a competitive inhibition kinetic ELISA to determine the inhibition constant (Ki) of monoclonal antibodies3
ELISA protein detector (EPD): A Python-based ELISA tool for accurate low-level protein quantification3
Immunoinformatics-guided designing of epitope-based subunit vaccine from Pilus assembly protein of Acinetobacter baumannii bacteria3
Identification of monoclonal antibodies from naive antibody phage-display libraries for protein detection in formalin-fixed paraffin-embedded tissues3
In silico engineering a CD80 variant with increased affinity to CTLA-4 and decreased affinity to CD28 for optimized cancer immunotherapy3
Chimeric protein A/G conjugate based lateral flow assay for the rapid detection of brucellosis in multiple animal species3
Differences in mouse strains determine the outcome of Der p 2 allergy induction protocols3
A taxonomy of multiple regression methods for immunologists3
Novel in vitro invariant natural killer T cell functional assays3
A standarized protocol for evaluation of large extracellular vesicles using the attune™ NXT system3
Gene knockout in highly purified mouse hematopoietic stem cells by CRISPR/Cas9 technology3
Editorial Board3
Evaluation of multiple immunoassay formats for detection of anti-drug antibodies to zinpentraxin alfa3
Qualification of ELISA and neutralization methodologies to measure SARS-CoV-2 humoral immunity using human clinical samples3
Repeatability of QuantiFERON-TB gold plus testing utilizing microparticle chemiluminescence3
Reference intervals for functional lymphocyte proliferation studies using 3H thymidine uptake in adults3
Platelets isolation and ectonucleotidase assay: Revealing functional aspects of the communication between the vasculature and the immune system3
Editorial Board3
A light-initiated chemiluminescent assay for the detection of children's milk protein-specific IgE with excellent ability to avoid interference of specific IgG3
PD-BAT: A novel approach of pooling basophil donors for expansion of commercial laboratory testing of Chronic Spontaneous Urticaria3
Chemiluminescent detection of platelet derived growth factor-BB based on sandwich label-free aptasensor and biotin–streptavidin strategy3
Multiplex immunoassay to measure antibody response to nine HPV vaccine types3
Application of microphysiologic system to assess neutrophil extracellular trap in xenotransplantation3
A dried blood spot test for diagnosis of autoimmune pulmonary alveolar proteinosis3
Expression of bluetongue virus full-length VP7 protein in insect cells and its diagnostic utility for detection of antibodies to the virus infection3
Theoretical and practical considerations for validating antigen-specific B cell ImmunoSpot assays3
Basophil activation test; User's manual3
Preparation and characterization of a neutralizing murine monoclonal antibody against tetanus toxin3
SARS-CoV-2 serology testing: Progress and challenges2
Simultaneous determination of inflammatory factors SAA and LTF based on stable element labeling and inductively coupled plasma mass spectrometry to aid in the diagnosis of infection2
Optimization of lateral flow assay for Canine morbillivirus detection and the application of the strip as sample substitute2
Isolation and culture of rat intestinal mucosal microvascular endothelial cells using immunomagnetic beads2
Methyl ester and aromatic ether modification of mitragynine for generation of mitragynine-specific polyclonal antibodies2
Antibody engineering to generate anti-tumor-associated glycoprotein 72 mouse recombinant CC49 IgG with improved solubility, purity, and thermal stability2
Rapid identification of tumor-reactive T-cell receptors by RNA preamplification-based single-cell sequencing2
Immunocartography: Charting vaccine-driven immunity by applying single cell proteomics to an in vitro human model2
SARS-CoV-2 spike protein expression as an identification in quality control testing for Adenovector based COVID-19 vaccine2
Editorial Board2
Clinical and analytical evaluation of the Abbott AdviseDx quantitative SARS-CoV-2 IgG assay and comparison with two other serological tests2
A novel immuno-molecular strategy for the detection of Streptococcus pneumoniae serotypes in human cerebrospinal and middle ear fluids2
Description of a non-competitive ELISA based on time course analysis of ligand binding at saturation, and a direct method for calculating the affinity of monoclonal antibodies2
Nanobody-based microfluidic human Fc assay for preclinical plasma quantification of IgG1/1.1 and IgG1-Fc-conjugates2
Development and evaluation of a low cost IgG ELISA test based in RBD protein for COVID-192
A flexible gradient lateral flow immunochromatographic assay for qualitative, semi-quantitative, and quantitative determination of serum amyloid A2
Editorial Board2
Generation of Q-bead against bone Gla protein with simplified preparation steps2
Discrepancies between two total IgE assays and difference in reference intervals in healthy adults2
Optimization of IgG1 immune complexes to stimulate cytokine production in innate cells2
Fresh versus frozen PMBC using the SARS CoV-2 T-SPOT.COVID test. Which works best?2
Stabilization of leukocytes from cerebrospinal fluid for central immunophenotypic evaluation in multicenter clinical trials2
Antibody detection by agglutination-PCR (ADAP) assays for the analysis of tissue transglutaminase autoantibodies in celiac disease2
Sources of variability in Luminex bead-based cytokine assays: Evidence from twelve years of multi-site proficiency testing2
Editorial – Special Issue on in vitro detection of drug allergy2
Editorial Board2
Optimizing the method for expressing human monoclonal antibodies from a single peripheral blood cell from vaccinated donors2
A rapid and high-throughput T cell immunophenotyping assay for cellular therapy bioprocess using the Cellaca® PLX image cytometer2
A method to isolate and cryopreserve cerebrospinal fluid mononuclear cells from external ventricular drains to investigate immunological processes in acute brain injuries2
Editorial Board2
Future perspectives on in-vitro diagnosis of drug allergy by the lymphocyte transformation test2
A flow cytometry-based proliferation assay for clinical evaluation of T-cell memory against SARS-CoV-22
A novel antigen immunochromatography fluorometric strip for rapid detection and application of pathogenic bacterial high-quality antibody2
Skeletal muscle macrophage ablation in mice2
An eight-plex immunoassay for Group A streptococcus serology and vaccine development2
Differential composition and yield of leukocytes isolated from various blood component leukoreduction filters2
Preparation of substrates for microarray protein chips with different ending functional groups2
Peptide Hamming Graphs: A network representation of peptides presented through specific HLAs to identify potential epitope clusters2
Impact of bridge heterology on functional parameters of ELISA for 17α-methyltestosterone2
Human monoclonal antibody cloning and expression with overlap extension PCR and short DNA fragments2
Comparison of methods to isolate peripheral blood mononuclear cells from cattle blood2
Validation for soluble C5b-9 detection and comparative analysis of three quantification methods2
Development of a rapid lateral flow immunochromatography assay for the detection of group-specific antibodies against VP7 protein of bluetongue virus in multiple species2
Development and laboratory validation of an electrochemiluminescence ELISA technique for measuring infliximab concentrations and anti-drug antibodies2
Identification of the linear Fc-binding epitope on the bovine IgG1 Fc receptor of (boFcγRI) using synthetic peptides2
Evaluation of a point of care lateral flow assay for antibody detection following SARS CoV-2 mRNA vaccine series2
Whole blood based point-of-care assay for the detection of anti-pertussis toxin IgG antibodies2
Novel IgE crosslinking-induced luciferase expression method using human-rat chimeric IgE receptor-carrying mast cells2
Development and performance evaluation of a novel, fully automated, high-throughput GAD65 autoantibody assay on the Phadia platform2
Application of the SARS-CoV-2-S1 ACE-2 receptor interaction as the basis of the fully automated assay to detect neutralizing SARS-CoV-2-S1 antibodies in blood samples2
A novel HRM-based gap-qRT-PCR for identification and quantitation of the vaccine and field strain(s) of lumpy skin disease virus2
The role of gamma globulin, complement component 1q, factor B, properdin, body temperature, C-reactive protein and serum amyloid alpha to the activity and the function of the human complement system a2
Fit-for-purpose validation of a drug-tolerant immunogenicity assay for a human mAb drug in animal safety studies2
Proficiency tests to evaluate the impact on assay outcomes of harmonized influenza-specific Intracellular Cytokine Staining (ICS) and IFN-ɣ Enzyme-Linked ImmunoSpot (ELISpot) protocols2
Comparison of mixed modeling regression methods for the assessment of longitudinal CyTOF® data2
Editorial Board2
Assessment of mimicking by EBV-CMV immunoglobulin M of anti-HLA antibodies2
Editorial Board2
Advanced hybridoma technology for selective production of high-affinity monoclonal antibodies through B-cell receptors2
Development and evaluation of a multi-antigen serological assay for the intra-vitam diagnosis of Tuberculosis caused by Mycobacterium bovis in pigs2
A homogeneous time-resolved fluorometric energy transfer assay for the binding assessment of FcRn with IgG antibodies2
Ultrasensitive multiplexed chemiluminescent enzyme-linked immunosorbent assays in 384-well plates1
Evaluation of a multiplexed coronavirus antigen array for detection of SARS-CoV-2 specific IgG in COVID-19 convalescent plasma1
Editorial Board1
Assessing TCR identity, knock-in efficiency, and potency for individualized TCR-T cell therapy1
Immunoassay for detection of oligomeric proteins1
Retention of E-selectin functionalized liposome fanny packs on Jurkat cells following invasion through collagen1
Comparison of single-chain variable fragments and monoclonal antibody against dihydroartemisinin1
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