Journal of Immunological Methods

Article	Citations
Development and validation of dengue virus envelope protein domain III IgG antibody enzyme-linked immunosorbent assay	62
Phospho-flow cytometry assays for diagnostic use – A discussion of assay utility and assay development and validation challenges	44
Decreased variability in the site-specific results during participation in the External Quality Assurance Program Oversight Laboratory (EQAPOL) proficiency program for IFN-gamma enzyme-linked immunosp	27
Antibodies against chikungunya virus structural proteins reveal the presence of CHIKV virions in migrasomes	22
Development of a new automated IL-6 immunoassay	22
Smartphone based lateral flow immunoassay quantifications	21
A FluoroSpot B assay for the detection of IgA and IgG SARS-CoV-2 spike-specific memory B cells: Optimization and qualification for use in COVID-19 vaccine trials	18
Establishing biological variation of immunological and inflammatory biomarkers in healthy individuals: a longitudinal study	18
Appropriate immunoassay systems are needed to evaluate various anticentromere antibodies as biomarkers for disease progression in systemic sclerosis	16
Inter-intra instrument comparison and standardization of a 10-color immunophenotyping for B and T cell non-Hodgkin lymphoma diagnosis and monitoring	16
Editorial Board	16
Candidate antibody reference reagents for Chlamydia trachomatis serology	16
Phage display selection of human single domain antibodies towards karilysin, a metalloproteinase and secreted virulence factor from Tannerella forsythia	15
Agreement and error of titration assays	15
Development of optimized cytotoxicity assays for assessing the antitumor potential of CAR-T cells	14
Combined RNAscope and immunohistochemistry staining on duodenal paraffin sections as a new tool to reveal cytolytic potential of intraepithelial lymphocytes	14
Challenges for complement functional assays in the clinical laboratory: From test validation to clinical interpretation	13
Techniques for evaluating the ATP-gated ion channel P2X7 receptor function in macrophages and microglial cells	13
Editorial Board	13
Comparability study of monocyte derived dendritic cells, primary monocytes, and THP1 cells for innate immune responses	12
Complete primer set for amplification and expression of full-length recombinant human monoclonal antibodies from single human B cells	12
A sensitive and drug tolerant assay for detecting anti-AAV9 antibodies using affinity capture elution	12
An unexpected bioanalytical challenge caused by positive control antibodies in a clinical immunogenicity assay – A simple solution and broadly applicable recommendations	11
Rapid, sensitive and cost-effective determination of immune checkpoint inhibitor activity using a magnetic bead-based binding assay	11
Development of an ELISA strategy for the serological diagnosis of farmer's lung disease	11

Humoral and cellular response of two different vaccines against SARS-CoV-2 in a group of healthcare workers: Comment	11
Quantitative flow cytometry using quantitative streptavidin-protein G-biotin beads (qBeads)	11
Editorial Board	11
Dried blood spots are a valid alternative to venipuncture for COVID-19 antibody testing	11
Development of automated microfluidic immunoassays for the detection of SARS-CoV-2 antibodies and antigen	11
Divalent cations and SERPIN gene expression modulate horseshoe crab coagulation under LPS induction	11
Flow cytometry reveals constant lymphocyte proportions after long-term cryopreservation of whole blood in TransFix® cell stabilization reagent	11
Automated EliA dsDNA assay: correlation with Crithidia lucillea indirect immunofluorescent test	11
Current technologies for anti-ENA antibody detection: State-of-the-art of diagnostic immunoassays	10
A systematic examination of anti-drug antibody titer estimation: Applied recommendations	10
Editorial Board	10
Humoral and cellular response of two different vaccines against SARS-CoV-2 in a group of healthcare workers: An observational study	9
Target tolerance improvement of the immunogenicity assay developed for analysing samples from clinical trials of RGB-14, a proposed biosimilar to Prolia/Xgeva	9
Optimisation of SARS-CoV-2 peptide stimulation and measurement of cytokine output by intracellular flow cytometry and bio-plex analysis	9
Leptospirosis detection: correspondence	9
Cross-reactivity of T cell-specific antibodies in the bank vole (Myodes glareolus)	9
Preparation and specific identification of monoclonal antibodies targeting the VP2 structural protein of minute virus of canines	9
Temperature stability of rabies neutralizing antibodies	8
Inducing and regulating human naive CD4+ T cell proliferation by different antigen presenting cells	8
Optimization of blood sampling time for isolation of antigen binding single-domain antibodies from immunized llamas	8
Development of sandwich enzyme-linked immunosorbent assays quantifying mouse urinary megalin, a novel proximal tubular biomarker	8
Comparison of real-time quantitative PCR and two digital PCR platforms to detect copy number variation in FCGR3B	8
Evaluation of self-collected dried blood spots for detection of SARS-CoV-2 nucleocapsid antibodies shows low sensitivity	8
Indirect ELISAs with sucrose subcellular fractions of Neospora caninum as antigens for diagnosis of neosporosis in cattle	8
A 33-color panel of phenotypic analysis of murine organ specific immune cells	8
Performance characteristics of the BRAHMS KRYPTOR automated squamous cell carcinoma antigen assay	8
Preliminary field evaluation of indirect ELISA test using the recombinant antigen rLicNTPDase-2 for serodiagnosis of canine leishmaniasis in Colombia	8
NADPH oxidase subunit p22 : A marker of oxidase-dependent oxidative stress and target for stress suppression in nonphagocytic cells	8
Persistence of criteria antiphospholipid antibodies in relation to titers, isotype and antiphospholipid syndrome diagnosis	7
A comprehensive method for the phenotypical and functional characterization of recalled human memory B and T cells specific to vaccine antigens	7
Comparative evaluation of Luminex based assays for detection of SARS-CoV-2 antibodies in a transplantation laboratory	7
High-performance immune diagnosis of tuberculosis: Use of phage display and synthetic peptide in an optimized experimental design	7
A cell-based subtractive panning strategy for selection of conformation-specific single-chain variable-fragment (scFv) against dimerization domain of EGFR	7
Murine model of steroid-resistant neutrophilic bronchial asthma as an attempt to simulate human pathology	7
Fragments of bombinakinin M exist in lipopolysaccharide-stimulated skin secretions of Bombina maxima and show lipopolysaccharide-neutralizing activity	7
Determination of echinococcosis IgG antibodies using magnetic bead-based chemiluminescence immunoassay	7
Comparative evaluation of pretreatment techniques for enhancing specificity in solid-phase bead-based immunoassays	7
Monoclonal antibody on-rate constant determined from time-course data of ligand binding by capture ELISA: Evaluation of eight data analysis methods	7
Evaluation and correlation between SARS-CoV-2 neutralizing and binding antibodies in convalescent and vaccinated subjects	7
Magnetic CAR T cell purification using an anti-G4S linker antibody	7
Development and assessment of a new bioassay for accurate quantification of Type I interferons induced by bovine respiratory viruses	6
Strategies to determine positive anti-SARS-CoV-2 memory T lymphocyte response during the evolution of an epidemic	6
Long term investigation of formulation buffers to mitigate stability issues of conjugated critical reagents	6
Editorial Board	6
Development of a high-throughput image cytometric screening method as a research tool for immunophenotypic characterization of patient samples from clinical studies	6
Nephelometry vs. Immunoturbidimetry assay: Analytical performance on IgG subclasses	6
Development and evaluation of i-Tracker risankizumab and i-Tracker anti-risankizumab kits: fast and innovative chemiluminescent assays for the monitoring of patients treated with Risankizumab	6
Performance characteristics of a vascular endothelial growth factor-D (VEGF-D) ELISA for diagnostic and therapeutic monitoring	6
Standardization, validation, and comparative evaluation of a faster and high-performance test for quantification of yellow fever neutralizing antibodies	6
A novel panel of peptides with diagnostic potential for serological identification of Borrelia burgdorferi sensu stricto, B. garinii and B. afzelii in human sera	6

Identifying therapeutic monoclonal antibodies using target protein collision electrophoresis reflex assay to separate the wheat from the chaff	6
A novel strategy to investigate the factors regulating the Treg to Tfr transition during acute viral infection	6
VaxArray immunoassay for the multiplexed quantification of poliovirus D-antigen	6
Development and optimization of a diluted whole blood ELISpot assay to test immune function	6
Improved accessibility of the high precision T-SPOT®.TB test for laboratories and patients through the automation of cell isolation	6
Evaluation of refractometry methods for estimating passive immunity status in neonatal foals	6
An optimized flow cytometry panel for classifying macrophage polarization	6
Analysis of the lot-to-lot evaluation process for enzyme-linked immunosorbent assays (ELISA)	6
Isolation and expansion of murine γδ T cells from mouse splenocytes	6
Development and validation of an in vitro titrimetric method for determination of classical swine fever viruses in PK-15 cells	6
Antisera against flagellin A or B inhibits Pseudomonas aeruginosa motility as measured by novel video microscopy assay	6
True volumetric counting of CD34+ cells using flow cytometry	6
Publisher's note: Challenges for complement functional assays in the clinical laboratory: From test validation to clinical interpretation	5
1,25-dihydroxyvitamin D3 augments low-dose PMA-based monocyte-to-macrophage differentiation in THP-1 cells	5
The Fas-mediated apoptosis assay: From concept to clinical application	5
Development of a proficiency testing program for HPV serology assays used to evaluate antibody responses in vaccine trials	5
Development of a Bak gene based standard curve for absolute quantification of BK virus in real time quantitative PCR assay and noninvasive diagnosis of BK virus nephropathy in kidney allograft recipie	5
Performance and validation of an adaptable multiplex assay for detection of serologic response to SARS-CoV-2 infection or vaccination	5
Validation of dried blood spot sampling for detecting SARS-CoV-2 antibodies and total immunoglobulins in a large cohort of asymptomatic young adults	5
Effect of storage time on peripheral blood mononuclear cell isolation from blood collected in vacutainer CPT™ tubes	5
A note of caution for using calmodulin antibodies	5
In silico designed Staphylococcus aureus B-cell multi-epitope vaccine did not elicit antibodies against target antigens suggesting multi-domain approach	5
Study of avidity-ELISA: Comparison of chaotropic agents, incubation temperature and affinity maturation after meningococcal immunization	5
Celiac disease risk stratification: correlation of HLA genotype and celiac-specific antibody positivity	5
Development and characterization of an ETV1 rabbit monoclonal antibody for the immunohistochemical detection of ETV1 expression in cancer tissue specimens	5
Production and characterization of novel monoclonal antibodies against outer membrane protein A (OmpA) of Acinetobacter baumannii	5
Overcoming cross-reactivity of antibodies against human lactate dehydrogenase	5
Methodological approaches to optimize multiplex oral fluid SARS-CoV-2 IgG assay performance and correlation with serologic and neutralizing antibody responses	5
Editorial Board	5
Miniaturized CAR knocked onto CD3ε extends TCR function with CAR specificity under control of endogenous TCR signaling cascade	5
Isolation of anti-Ancylostoma-secreted protein 5 (ASP5) antibody from a naïve antibody phage library	4
Letter to the editor concerning “Development and validation of indirect ELISA for antibody detection against different protein antigens of porcine epidemic diarrhea virus in the colostrum and milk of	4
Cell-type-specific gene expression profile by laser capture microdissection on mirror sections	4
Comparison of two anti-histones antibodies commercial assays in an immunology laboratory and systemic lupus erythematosus	4
Artificial intelligence and deep learning to map immune cell types in inflamed human tissue	4
Optimized flow cytometry protocol for dihydrorhodamine 123-based detection of reactive oxygen species in leukocyte subpopulations in whole blood	4
Rapid, sensitive, and specific lateral-flow immunochromatographic point-of-care device for detection of varicella-zoster virus immunoglobulin G antibodies in fingerstick blood	4
Evaluation of cell-mediated immunity of E.coli nanovaccines in chickens	4
Acute kidney allograft dysfunction is associated with increased number of non-HLA antibodies in absence of donor specific HLA antibodies	4
Sensitive amplified luminescent proximity homogeneous assay for the quantitative detection of CA242	4
Stochastic interpretation of basophil activation test improves diagnostic reliability	4
Development of an enteric pathogen multiplex immunoassay to measure antibody responses in blood and saliva for integrated serology applications	4
Detection of SARS-CoV-2 peptide-specific antibodies in Syrian hamster serum by ELISA	4
A universal urinary cell gene signature of acute rejection in kidney allografts	4
Editorial Board	4
Tumor nano-lysate activates dendritic cells to evoke a preventative immune response	4
Editorial Board	4
30-color full spectrum flow cytometry panel for deep immunophenotyping of T cell subsets in murine tumor tissue	4
Validation of the performance and suitability of a new class of FBS optimized for use in single-cell functional assays	4
An enzyme-free method for isolating testicular macrophages from rodent models	4
Clinical utility of the lymphocyte proliferation assay, an in vitro functional readout of the adaptive immune response	4
Dissociation of murine oral mucosal tissues for single cell applications	4
A peptide derived from the N-terminal of NS2A for the preparation of ZIKV NS2A recognition polyclonal antibody	4
Flow cytometry for B-cell subset analysis in immunodeficiencies	4
Diagnosis of septic shock by serum measurement of human neutrophil lipocalin by a rapid homogeneous assay	4
Generation of an alpaca serum that induces immune-mediated crescentic glomerulonephritis in mice	4
Plaque reduction neutralization test for smallpox vaccines: Laboratory optimization and validation method for immunogenicity assessment	4
Editorial Board	4
Establishment of growth stimulating gene 2 protein time-resolved fluorescence immunoassay and its application in sepsis	4
Editorial Board	4
A standarized protocol for evaluation of large extracellular vesicles using the attune™ NXT system	3
ELISA protein detector (EPD): A Python-based ELISA tool for accurate low-level protein quantification	3
In silico engineering a CD80 variant with increased affinity to CTLA-4 and decreased affinity to CD28 for optimized cancer immunotherapy	3
Mayaro virus and chikungunya virus IgM and IgG testing in parallel improves diagnosis of acute Mayaro virus infections	3
A taxonomy of multiple regression methods for immunologists	3
Application of a caprylate/chromatography purification process for production of a high potency rabies immune globulin from pooled human plasma	3
Novel bridge multi-species ELISA for detection of SARS-CoV-2 antibodies	3
Chimeric protein A/G conjugate based lateral flow assay for the rapid detection of brucellosis in multiple animal species	3
Differences in mouse strains determine the outcome of Der p 2 allergy induction protocols	3
Complement function and activation in human serum and plasma collected in different blood collection tubes	3
Assessment of TREC-based NBS SCID reporting practices for harmonization of results and interpretation: a global survey	3
Highly sensitive detection of carcinoembryonic antigen via an electrochemical platform fabricated by CCLP- gold nanocomposite	3
Qualification of ELISA and neutralization methodologies to measure SARS-CoV-2 humoral immunity using human clinical samples	3
Immunoinformatics-guided designing of epitope-based subunit vaccine from Pilus assembly protein of Acinetobacter baumannii bacteria	3
Identification of monoclonal antibodies from naive antibody phage-display libraries for protein detection in formalin-fixed paraffin-embedded tissues	3
Editorial Board	3
Application of microphysiologic system to assess neutrophil extracellular trap in xenotransplantation	3
Novel in vitro invariant natural killer T cell functional assays	3
Theoretical and practical considerations for validating antigen-specific B cell ImmunoSpot assays	3
Reference intervals for functional lymphocyte proliferation studies using 3H thymidine uptake in adults	3

Basophil activation test; User's manual	3
Multiplex ACE2-RBD binding inhibition assay: An integrated tool for assessing neutralizing antibodies to SARS-CoV-2 variants and protection against breakthrough infections	3
A flow cytometry-based assay to determine the ability of anti-Streptococcus pyogenes antibodies to mediate monocytic phagocytosis in human sera	3
Multiplex immunoassay to measure antibody response to nine HPV vaccine types	3
A switchable secrete-and-capture system enables efficient selection of Pichia pastoris clones producing high yields of Fab fragments	3
Platelets isolation and ectonucleotidase assay: Revealing functional aspects of the communication between the vasculature and the immune system	3
Data to determination: transitioning to new methodology for better patient care, a process improvement journey of a clinical immunology laboratory in a pediatric hospital	3
Development of a novel anti-PEG antibody assay enabling investigation of potential immunogenicity triggered by the PEG moiety of biotherapeutics	3
A dried blood spot test for diagnosis of autoimmune pulmonary alveolar proteinosis	3
COVID-19 in patients with B cell immune deficiency	3
A comprehensive multiparameter flow cytometry panel for immune profiling and functional studies of frozen tissue, bone marrow, and spleen	3
Chemiluminescent detection of platelet derived growth factor-BB based on sandwich label-free aptasensor and biotin–streptavidin strategy	3
Preparation and characterization of a neutralizing murine monoclonal antibody against tetanus toxin	3
Optimized flow cytometry panel for the detection and analysis of human tumor-induced memory-like NK cells	3
Evaluation of multiple immunoassay formats for detection of anti-drug antibodies to zinpentraxin alfa	3
The mouse monoclonal antibody 4E3 is specific for the G1m17 allotype of human IgG1	3
Evaluation of commercially available antibodies and fluorescent conotoxins for the detection of surface ganglionic acetylcholine receptor on the neuroblastoma cell line, IMR-32 by flow cytometry	3
Highly effective strategy for isolation of mononuclear cells from frozen cord blood	3
PD-BAT: A novel approach of pooling basophil donors for expansion of commercial laboratory testing of Chronic Spontaneous Urticaria	3
Editorial Board	2
Preparation of substrates for microarray protein chips with different ending functional groups	2
A rapid and high-throughput T cell immunophenotyping assay for cellular therapy bioprocess using the Cellaca® PLX image cytometer	2
Fresh versus frozen PMBC using the SARS CoV-2 T-SPOT.COVID test. Which works best?	2
Indirect ELISA diagnostic based on recombinant 3AB3 nonstructural protein of foot-and-mouth disease virus for use in porcine serology	2
Comparison of mixed modeling regression methods for the assessment of longitudinal CyTOF® data	2
Clinical and analytical evaluation of the Abbott AdviseDx quantitative SARS-CoV-2 IgG assay and comparison with two other serological tests	2
Rapid identification of tumor-reactive T-cell receptors by RNA preamplification-based single-cell sequencing	2
SARS-CoV-2 spike protein expression as an identification in quality control testing for Adenovector based COVID-19 vaccine	2
Comparison of two chagas ELISA methods: why are the signal to cutoff ratios important?	2
Development and performance evaluation of a novel, fully automated, high-throughput GAD65 autoantibody assay on the Phadia platform	2
Optimization of IgG1 immune complexes to stimulate cytokine production in innate cells	2
Expression of bluetongue virus full-length VP7 protein in insect cells and its diagnostic utility for detection of antibodies to the virus infection	2
Editorial Board	2
Editorial Board	2
Editorial Board	2
Detection of autoantibodies in bullous pemphigoid, epidermolysis bullosa acquisita, and pemphigus vulgaris by indirect immunofluorescence with a BIOCHIP Mosaic	2
Comparison of methods to isolate peripheral blood mononuclear cells from cattle blood	2
Optimization of lateral flow assay for Canine morbillivirus detection and the application of the strip as sample substitute	2
Identification of the linear Fc-binding epitope on the bovine IgG1 Fc receptor of (boFcγRI) using synthetic peptides	2
Development and evaluation of a low cost IgG ELISA test based in RBD protein for COVID-19	2
Skeletal muscle macrophage ablation in mice	2
Development and evaluation of a multi-antigen serological assay for the intra-vitam diagnosis of Tuberculosis caused by Mycobacterium bovis in pigs	2
A flow cytometry-based proliferation assay for clinical evaluation of T-cell memory against SARS-CoV-2	2
A novel immuno-molecular strategy for the detection of Streptococcus pneumoniae serotypes in human cerebrospinal and middle ear fluids	2
Sources of variability in Luminex bead-based cytokine assays: Evidence from twelve years of multi-site proficiency testing	2
Advanced hybridoma technology for selective production of high-affinity monoclonal antibodies through B-cell receptors	2
A flexible gradient lateral flow immunochromatographic assay for qualitative, semi-quantitative, and quantitative determination of serum amyloid A	2
Stabilization of leukocytes from cerebrospinal fluid for central immunophenotypic evaluation in multicenter clinical trials	2
Generation of Q-bead against bone Gla protein with simplified preparation steps	2
Immunogenicity of tislelizumab: clinical summary of ADA incidence and feasibility of assessing the suitability of validation cut points using statistical methods	2
Impact of bridge heterology on functional parameters of ELISA for 17α-methyltestosterone	2
Description of a non-competitive ELISA based on time course analysis of ligand binding at saturation, and a direct method for calculating the affinity of monoclonal antibodies	2
Development of a rapid lateral flow immunochromatography assay for the detection of group-specific antibodies against VP7 protein of bluetongue virus in multiple species	2
Fit-for-purpose validation of a drug-tolerant immunogenicity assay for a human mAb drug in animal safety studies	2
Proficiency tests to evaluate the impact on assay outcomes of harmonized influenza-specific Intracellular Cytokine Staining (ICS) and IFN-ɣ Enzyme-Linked ImmunoSpot (ELISpot) protocols	2
The role of gamma globulin, complement component 1q, factor B, properdin, body temperature, C-reactive protein and serum amyloid alpha to the activity and the function of the human complement system a	2
A high-throughput assay using dysfunctional T cells for phenotypic screening immune checkpoint modulators	2
An eight-plex immunoassay for Group A streptococcus serology and vaccine development	2
Discrepancies between two total IgE assays and difference in reference intervals in healthy adults	2
Methyl ester and aromatic ether modification of mitragynine for generation of mitragynine-specific polyclonal antibodies	2
A novel HRM-based gap-qRT-PCR for identification and quantitation of the vaccine and field strain(s) of lumpy skin disease virus	2
Whole blood based point-of-care assay for the detection of anti-pertussis toxin IgG antibodies	2
Editorial Board	2
Shifting beyond the gel: comparison of therapeutic antibody gel shift assays to mass spectrometry for evaluating multiple myeloma patient serum samples	2
Editorial Board	2
Human monoclonal antibody cloning and expression with overlap extension PCR and short DNA fragments	2
Repeatability of QuantiFERON-TB gold plus testing utilizing microparticle chemiluminescence	2
Comparison of multiplexed protein analysis platforms for the detection of biomarkers in the nasal epithelial lining fluid of healthy subjects	1
Multiplex agglutination-PCR (ADAP) autoantibody assays compared to radiobinding autoantibodies in type 1 diabetes and celiac disease	1
Single-tiered serological diagnostic test for Lyme disease	1
Broad immunophenotyping of the murine brain tumor microenvironment	1
Editorial Board	1
Simultaneous multiple target detection platform based on vertical flow immunoassay	1
Peripheral blood and nasal swabs Type I and III IFNs signature in RSV positive infant with bronchiolitis	1
Editorial Board	1
From phlebotomy to peripheral blood mononuclear cell isolation; effect of time and early-life HIV/ART exposure on cell yield and viability in paediatric samples in a high HIV prevalence setting	1
Potential impact of SARS COV-2 infection on the performance of serological assays used to diagnose arboviral diseases	1
The spike-ACE2 binding assay: An in vitro platform for evaluating vaccination efficacy and for screening SARS-CoV-2 inhibitors and neutralizing antibodies	1
Development of a sensitive sandwich ELISA for the envelope domain III protein of dengue virus type 2	1
Editorial Board	1
Editorial Board	1
Development of a time-resolved fluorescence immunoassay based on immunomagnetic beads for gastrin-17	1
Development of in vitro plaque-based assay for assessment of antibody-dependent enhancement in SARS-CoV-2 infection	1
Editorial Board	1
Editorial Board	1
MARMOT: A multifaceted R pipeline for analysing spectral flow cytometry data from subcutaneously growing murine gastric organoids	1
Using multiple autoantibody biomarkers in diagnosing patients with rheumatoid arthritis: preliminary results from a single center	1
Repurposing discarded leukodepletion filters as a source of mononuclear cells for advanced in vitro research	1
Comparative assessment of a multiplex micro-chip immunoassay, VaxArray, and meso scale discovery assay for serotype-specific coronavirus IgG quantitation	1
SCHNAPPs - Single Cell sHiNy APPlication(s)	1
Development of a high-sensitivity assay for direct quantitation of EGFr on extracellular vesicles in plasma	1
Belimumab concentration measurements using a homologous anti-idiotype immunoassay	1
Evaluation of the concordance between GluN1-GluN2 heteromer live-cell-based assay and GluN1 monomer biochip kit assay on anti-NMDAR autoantibody detection	1
Development of a multiplexing potency assay using upconverting nanoparticles-based luminescence resonance energy transfer	1
Immunoglobulin treatment for B-cell immunodeficiencies	1