OOIR: Observatory of International Research

Papers

(The TQCC of Journal of Immunological Methods is 4. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-08-01 to 2025-08-01.)

Article	Citations
Editorial Board	61
Inter-intra instrument comparison and standardization of a 10-color immunophenotyping for B and T cell non-Hodgkin lymphoma diagnosis and monitoring	38
Appropriate immunoassay systems are needed to evaluate various anticentromere antibodies as biomarkers for disease progression in systemic sclerosis	27
Combined RNAscope and immunohistochemistry staining on duodenal paraffin sections as a new tool to reveal cytolytic potential of intraepithelial lymphocytes	25
Phospho-flow cytometry assays for diagnostic use – A discussion of assay utility and assay development and validation challenges	22
Agreement and error of titration assays	21
A FluoroSpot B assay for the detection of IgA and IgG SARS-CoV-2 spike-specific memory B cells: Optimization and qualification for use in COVID-19 vaccine trials	21
Development of a new automated IL-6 immunoassay	20
Decreased variability in the site-specific results during participation in the External Quality Assurance Program Oversight Laboratory (EQAPOL) proficiency program for IFN-gamma enzyme-linked immunosp	18
Optimized cytotoxicity assay for co-suspended effector and target cells	18
Candidate antibody reference reagents for Chlamydia trachomatis serology	17
Phage display selection of human single domain antibodies towards karilysin, a metalloproteinase and secreted virulence factor from Tannerella forsythia	17
Antibodies against chikungunya virus structural proteins reveal the presence of CHIKV virions in migrasomes	16
Editorial Board	16
Techniques for evaluating the ATP-gated ion channel P2X7 receptor function in macrophages and microglial cells	15
A sensitive and drug tolerant assay for detecting anti-AAV9 antibodies using affinity capture elution	15
Comparability study of monocyte derived dendritic cells, primary monocytes, and THP1 cells for innate immune responses	15
Development of optimized cytotoxicity assays for assessing the antitumor potential of CAR-T cells	14
Challenges for complement functional assays in the clinical laboratory: From test validation to clinical interpretation	14
Editorial Board	13
Smartphone based lateral flow immunoassay quantifications	13
Rapid, sensitive and cost-effective determination of immune checkpoint inhibitor activity using a magnetic bead-based binding assay	13
Development and validation of dengue virus envelope protein domain III IgG antibody enzyme-linked immunosorbent assay	13
Dried blood spots are a valid alternative to venipuncture for COVID-19 antibody testing	12
Development of an ELISA strategy for the serological diagnosis of farmer's lung disease	11

Immunoinformatics and Pepscan strategies on the path of a peptide-based serological diagnosis of COVID19	11
Flow cytometry reveals constant lymphocyte proportions after long-term cryopreservation of whole blood in TransFix® cell stabilization reagent	11
Optimisation of SARS-CoV-2 peptide stimulation and measurement of cytokine output by intracellular flow cytometry and bio-plex analysis	11
Divalent cations and SERPIN gene expression modulate horseshoe crab coagulation under LPS induction	11
Quantitative flow cytometry using quantitative streptavidin-protein G-biotin beads (qBeads)	11
Target tolerance improvement of the immunogenicity assay developed for analysing samples from clinical trials of RGB-14, a proposed biosimilar to Prolia/Xgeva	11
Complete primer set for amplification and expression of full-length recombinant human monoclonal antibodies from single human B cells	11
An unexpected bioanalytical challenge caused by positive control antibodies in a clinical immunogenicity assay – A simple solution and broadly applicable recommendations	11
Leptospirosis detection: correspondence	10
Performance characteristics of the BRAHMS KRYPTOR automated squamous cell carcinoma antigen assay	10
Current technologies for anti-ENA antibody detection: State-of-the-art of diagnostic immunoassays	10
Editorial Board	10
Development of automated microfluidic immunoassays for the detection of SARS-CoV-2 antibodies and antigen	10
A systematic examination of anti-drug antibody titer estimation: Applied recommendations	9
Evaluation of self-collected dried blood spots for detection of SARS-CoV-2 nucleocapsid antibodies shows low sensitivity	9
Cocaine binding to the Fab fragment of a humanized anti-cocaine mAb quantitated by dye absorption and fluorescence spectroscopy	9
Humoral and cellular response of two different vaccines against SARS-CoV-2 in a group of healthcare workers: Comment	9
Preparation and specific identification of monoclonal antibodies targeting the VP2 structural protein of minute virus of canines	9
Cross-reactivity of T cell-specific antibodies in the bank vole (Myodes glareolus)	9
Autotransporter-based surface expression and complementation of split TreA fragments utilized for the detection of antibodies against bovine leukemia virus	9
Comparison of real-time quantitative PCR and two digital PCR platforms to detect copy number variation in FCGR3B	9
Humoral and cellular response of two different vaccines against SARS-CoV-2 in a group of healthcare workers: An observational study	8
High-performance immune diagnosis of tuberculosis: Use of phage display and synthetic peptide in an optimized experimental design	8
Inducing and regulating human naive CD4+ T cell proliferation by different antigen presenting cells	8
Evaluation and correlation between SARS-CoV-2 neutralizing and binding antibodies in convalescent and vaccinated subjects	8
A 33-color panel of phenotypic analysis of murine organ specific immune cells	8
Fragments of bombinakinin M exist in lipopolysaccharide-stimulated skin secretions of Bombina maxima and show lipopolysaccharide-neutralizing activity	8
Performance of a commercially available multiplex platform in the assessment of circulating cytokines and chemokines in patients with rheumatoid arthritis and osteoarthritis	7
True volumetric counting of CD34+ cells using flow cytometry	7
NADPH oxidase subunit p22 : A marker of oxidase-dependent oxidative stress and target for stress suppression in nonphagocytic cells	7
A comprehensive method for the phenotypical and functional characterization of recalled human memory B and T cells specific to vaccine antigens	7
Magnetic CAR T cell purification using an anti-G4S linker antibody	7
Long term investigation of formulation buffers to mitigate stability issues of conjugated critical reagents	7
Determination of echinococcosis IgG antibodies using magnetic bead-based chemiluminescence immunoassay	7
A novel strategy to investigate the factors regulating the Treg to Tfr transition during acute viral infection	7
Indirect ELISAs with sucrose subcellular fractions of Neospora caninum as antigens for diagnosis of neosporosis in cattle	7
Identifying therapeutic monoclonal antibodies using target protein collision electrophoresis reflex assay to separate the wheat from the chaff	7
Murine model of steroid-resistant neutrophilic bronchial asthma as an attempt to simulate human pathology	7
Monoclonal antibody on-rate constant determined from time-course data of ligand binding by capture ELISA: Evaluation of eight data analysis methods	7
Comparative evaluation of Luminex based assays for detection of SARS-CoV-2 antibodies in a transplantation laboratory	7
Development and assessment of a new bioassay for accurate quantification of Type I interferons induced by bovine respiratory viruses	7
Effect of storage time on peripheral blood mononuclear cell isolation from blood collected in vacutainer CPT™ tubes	7
Optimization of blood sampling time for isolation of antigen binding single-domain antibodies from immunized llamas	7
Preliminary field evaluation of indirect ELISA test using the recombinant antigen rLicNTPDase-2 for serodiagnosis of canine leishmaniasis in Colombia	7
Development of sandwich enzyme-linked immunosorbent assays quantifying mouse urinary megalin, a novel proximal tubular biomarker	7
A cell-based subtractive panning strategy for selection of conformation-specific single-chain variable-fragment (scFv) against dimerization domain of EGFR	7
An optimized flow cytometry panel for classifying macrophage polarization	7
Strategies to determine positive anti-SARS-CoV-2 memory T lymphocyte response during the evolution of an epidemic	6
Standardization, validation, and comparative evaluation of a faster and high-performance test for quantification of yellow fever neutralizing antibodies	6
Overcoming cross-reactivity of antibodies against human lactate dehydrogenase	6

Development of a Bak gene based standard curve for absolute quantification of BK virus in real time quantitative PCR assay and noninvasive diagnosis of BK virus nephropathy in kidney allograft recipie	6
Methodological approaches to optimize multiplex oral fluid SARS-CoV-2 IgG assay performance and correlation with serologic and neutralizing antibody responses	6
Validation of dried blood spot sampling for detecting SARS-CoV-2 antibodies and total immunoglobulins in a large cohort of asymptomatic young adults	6
A novel panel of peptides with diagnostic potential for serological identification of Borrelia burgdorferi sensu stricto, B. garinii and B. afzelii in human sera	6
Nephelometry vs. Immunoturbidimetry assay: Analytical performance on IgG subclasses	6
1,25-dihydroxyvitamin D3 augments low-dose PMA-based monocyte-to-macrophage differentiation in THP-1 cells	6
Evaluation of refractometry methods for estimating passive immunity status in neonatal foals	6
Isolation and expansion of murine γδ T cells from mouse splenocytes	6
The Fas-mediated apoptosis assay: From concept to clinical application	6
A note of caution for using calmodulin antibodies	6
Production and characterization of novel monoclonal antibodies against outer membrane protein A (OmpA) of Acinetobacter baumannii	6
VaxArray immunoassay for the multiplexed quantification of poliovirus D-antigen	6
In silico designed Staphylococcus aureus B-cell multi-epitope vaccine did not elicit antibodies against target antigens suggesting multi-domain approach	6
Editorial Board	6
Development and optimization of a diluted whole blood ELISpot assay to test immune function	6
Antisera against flagellin A or B inhibits Pseudomonas aeruginosa motility as measured by novel video microscopy assay	6
Editorial Board	6
Development and characterization of an ETV1 rabbit monoclonal antibody for the immunohistochemical detection of ETV1 expression in cancer tissue specimens	6
Development and validation of an in vitro titrimetric method for determination of classical swine fever viruses in PK-15 cells	6
Development of a high-throughput image cytometric screening method as a research tool for immunophenotypic characterization of patient samples from clinical studies	6
Performance and validation of an adaptable multiplex assay for detection of serologic response to SARS-CoV-2 infection or vaccination	5
30-color full spectrum flow cytometry panel for deep immunophenotyping of T cell subsets in murine tumor tissue	5
Study of avidity-ELISA: Comparison of chaotropic agents, incubation temperature and affinity maturation after meningococcal immunization	5
Clinical utility of the lymphocyte proliferation assay, an in vitro functional readout of the adaptive immune response	5
Editorial Board	5
Generation of an alpaca serum that induces immune-mediated crescentic glomerulonephritis in mice	5
Validation of the performance and suitability of a new class of FBS optimized for use in single-cell functional assays	5
Editorial Board	5
Development of a proficiency testing program for HPV serology assays used to evaluate antibody responses in vaccine trials	5
Publisher's note: Challenges for complement functional assays in the clinical laboratory: From test validation to clinical interpretation	5
Evaluation of cell-mediated immunity of E.coli nanovaccines in chickens	5
Artificial intelligence and deep learning to map immune cell types in inflamed human tissue	5
Rapid, sensitive, and specific lateral-flow immunochromatographic point-of-care device for detection of varicella-zoster virus immunoglobulin G antibodies in fingerstick blood	5
Diagnosis of septic shock by serum measurement of human neutrophil lipocalin by a rapid homogeneous assay	5
Establishment of growth stimulating gene 2 protein time-resolved fluorescence immunoassay and its application in sepsis	5
An enzyme-free method for isolating testicular macrophages from rodent models	5
Miniaturized CAR knocked onto CD3ε extends TCR function with CAR specificity under control of endogenous TCR signaling cascade	5
Sensitive amplified luminescent proximity homogeneous assay for the quantitative detection of CA242	5
Detection of drug-specific immunoglobulin E (IgE) and acute mediator release for the diagnosis of immediate drug hypersensitivity reactions	5
Editorial Board	5
Plaque reduction neutralization test for smallpox vaccines: Laboratory optimization and validation method for immunogenicity assessment	5
Optimisation and validation of a new method for antibody dependent cellular phagocytosis in hepatitis C virus infection	5
Editorial Board	5
The mouse monoclonal antibody 4E3 is specific for the G1m17 allotype of human IgG1	4
Isolation of anti-Ancylostoma-secreted protein 5 (ASP5) antibody from a naïve antibody phage library	4
Comparison of two anti-histones antibodies commercial assays in an immunology laboratory and systemic lupus erythematosus	4
Development of an enteric pathogen multiplex immunoassay to measure antibody responses in blood and saliva for integrated serology applications	4
Complement function and activation in human serum and plasma collected in different blood collection tubes	4
Immunoinformatics-guided designing of epitope-based subunit vaccine from Pilus assembly protein of Acinetobacter baumannii bacteria	4
Highly sensitive detection of carcinoembryonic antigen via an electrochemical platform fabricated by CCLP- gold nanocomposite	4
PD-BAT: A novel approach of pooling basophil donors for expansion of commercial laboratory testing of Chronic Spontaneous Urticaria	4
A peptide derived from the N-terminal of NS2A for the preparation of ZIKV NS2A recognition polyclonal antibody	4
Evaluation of commercially available antibodies and fluorescent conotoxins for the detection of surface ganglionic acetylcholine receptor on the neuroblastoma cell line, IMR-32 by flow cytometry	4
Hybrid recombinant Omp 22, 25, and 31 immunodominant epitopes can be used for serodiagnosis of brucellosis	4
Highly effective strategy for isolation of mononuclear cells from frozen cord blood	4
Optimized flow cytometry protocol for dihydrorhodamine 123-based detection of reactive oxygen species in leukocyte subpopulations in whole blood	4
Tumor nano-lysate activates dendritic cells to evoke a preventative immune response	4
A flow cytometry-based assay to determine the ability of anti-Streptococcus pyogenes antibodies to mediate monocytic phagocytosis in human sera	4
A universal urinary cell gene signature of acute rejection in kidney allografts	4
Basophil activation test; User's manual	4
A taxonomy of multiple regression methods for immunologists	4
COVID-19 in patients with B cell immune deficiency	4
ELISpot assay as a diagnostic tool in drug hypersensitivity reactions	4
Letter to the editor concerning “Development and validation of indirect ELISA for antibody detection against different protein antigens of porcine epidemic diarrhea virus in the colostrum and milk of	4
Cell-type-specific gene expression profile by laser capture microdissection on mirror sections	4
Flow cytometry for B-cell subset analysis in immunodeficiencies	4
Dissociation of murine oral mucosal tissues for single cell applications	4
A switchable secrete-and-capture system enables efficient selection of Pichia pastoris clones producing high yields of Fab fragments	4
Reference intervals for functional lymphocyte proliferation studies using 3H thymidine uptake in adults	4
A standarized protocol for evaluation of large extracellular vesicles using the attune™ NXT system	4
Novel bridge multi-species ELISA for detection of SARS-CoV-2 antibodies	4
Qualification of ELISA and neutralization methodologies to measure SARS-CoV-2 humoral immunity using human clinical samples	4
Multiplex immunoassay to measure antibody response to nine HPV vaccine types	4
Novel in vitro invariant natural killer T cell functional assays	4
Detection of SARS-CoV-2 peptide-specific antibodies in Syrian hamster serum by ELISA	4