Journal of Immunological Methods

Article	Citations
Publisher's note: Challenges for complement functional assays in the clinical laboratory: From test validation to clinical interpretation	58
Appropriate immunoassay systems are needed to evaluate various anticentromere antibodies as biomarkers for disease progression in systemic sclerosis	55
Editorial Board	34
A novel method for in vitro culture and expansion of nonhuman primate B cells	29
Editorial Board	25
Inter-intra instrument comparison and standardization of a 10-color immunophenotyping for B and T cell non-Hodgkin lymphoma diagnosis and monitoring	21
Immunoglobulin isotype compositions of ABO specific antibodies are dependent on the individual patient blood group and blood group specificity: Results from a healthy donor cohort	19
Establishment of a novel cell-based assay using HLA-transfected cells to detect HLA antibodies	18
Role of antinuclear antibodies in COVID-19 patients	18
A sensitive and less cytotoxic assay for identification of proliferating T cells based on bioorthogonally-functionalized uridine analogue	18
Editorial Board	16
Characterization and ex vivo expansion of rare in situ cytokine secreting T cell populations from tumor tissue and blood of oral squamous cell carcinoma patients	16
Comparability study of monocyte derived dendritic cells, primary monocytes, and THP1 cells for innate immune responses	15
A single homogeneous assay for simultaneous measurement of bispecific antibody target binding	15
A rapid and high-throughput T cell immunophenotyping assay for cellular therapy bioprocess using the Cellaca® PLX image cytometer	14
Thanking the reviewers of Journal of Immunological Methods	14
Editorial Board	14
Combined RNAscope and immunohistochemistry staining on duodenal paraffin sections as a new tool to reveal cytolytic potential of intraepithelial lymphocytes	13
Editorial Board	13
An enzyme-free method for isolating testicular macrophages from rodent models	13
Editorial Board	13
Recombinant antigen 5 from Polybia paulista wasp venom (Hymenoptera, Vespidae): Antigen-specific antibody production and functional profile of CD4+ T cells in the immune response	13
Editorial introduction for “B cell immunodeficiencies” Journal of Immunological Methods special edition	13
Detection of drug-specific immunoglobulin E (IgE) and acute mediator release for the diagnosis of immediate drug hypersensitivity reactions	11
Development of competitive inhibition ELISA as an effective potency test to analyze human rabies vaccines and assessment of the antigenic epitope of rabies glycoprotein	11

Quantitative determination of C-polysaccharide in Streptococcus pneumoniae capsular polysaccharides by enzyme-linked immunosorbent assay	11
Feasibility of using monocyte-derived dendritic cells obtained from cryopreserved cells for DC-based vaccines	11
Evaluation of cell-mediated immunity of E.coli nanovaccines in chickens	10
Challenges for complement functional assays in the clinical laboratory: From test validation to clinical interpretation	10
Development of an indirect competitive enzyme linked immunosorbent assay for the quantitative detection of Mycoplasma hyopneumoniae during the vaccine production process	10
Development of a flow cytometry-based potency assay for prediction of cytokine storms induced by biosimilar monoclonal antibodies	10
Discrepancies between two total IgE assays and difference in reference intervals in healthy adults	10
SARS-CoV-2 spike protein expression as an identification in quality control testing for Adenovector based COVID-19 vaccine	10
Development of gold nanocluster complex for the detection of tumor necrosis factor-alpha based on immunoassay	10
Development of a defibrinated human blood hemolysis assay for rapid testing of hemolytic activity compared to computational prediction	10
Robust and low-cost ELISA based on IgG-Fc tagged recombinant proteins to screen for anti-SARS-CoV-2 antibodies	10
Phospho-flow cytometry assays for diagnostic use – A discussion of assay utility and assay development and validation challenges	10
Clinical utility of the lymphocyte proliferation assay, an in vitro functional readout of the adaptive immune response	10
Development of immunochromatographic strip assay to detect recent infection of Japanese encephalitis virus in swine population	9
Selection of positive controls and their impact on anti-drug antibody assay performance	9
Decreased variability in the site-specific results during participation in the External Quality Assurance Program Oversight Laboratory (EQAPOL) proficiency program for IFN-gamma enzyme-linked immunosp	9
The generation of a novel HER2-targeting nano PE25 immunotoxin with superior anti-tumor activity and high productivity	9
Digital RT-PCR Chip method for detection of SARS-CoV-2 virus	9
Parameters of immunoglobulin extraction from dried blood spot cards and immunoassays for detection of antibody response to pathogens including the novel SARS-CoV-2	9
Establishment of growth stimulating gene 2 protein time-resolved fluorescence immunoassay and its application in sepsis	9
A sensitive method to quantify HIV-1 antibodies in mucosal samples	9
Immunoassay for detection of oligomeric proteins	9
Construction of a full-length antibody phage display vector	8
Phage display selection of human single domain antibodies towards karilysin, a metalloproteinase and secreted virulence factor from Tannerella forsythia	8
Critical evaluation of fluorescent dyes to evaluate the stability and ligand binding properties of an anti-cocaine mAb, h2E2	8
A chimeric HLA-A2:β2M:Ig fusion protein for the study of virus-specific CD8+ T-cells	8
Establishment of a homogeneous immunoassay-light-initiated chemiluminescence assay for detecting anti-Müllerian hormone in human serum	8
Validation of the performance and suitability of a new class of FBS optimized for use in single-cell functional assays	8
Dried blood spots for the quantitative evaluation of IgG isotypes and correlation with serum samples in HIV-exposed uninfected (HEU) infants	8
Immunodetection of Furcraea Necrotic Streak Virus-FNSV in fique plants (Furcraea macrophylla Baker) using a polyclonal antibody IgY produced in chicken egg yolk	8
Interactions of human mesenchymal stromal cells with peripheral blood mononuclear cells in a Mitogenic proliferation assay	7
Optimization of lateral flow assay for Canine morbillivirus detection and the application of the strip as sample substitute	7
Optimized cytotoxicity assay for co-suspended effector and target cells	7
A SMART method for isolating monoclonal antibodies from individual rhesus macaque memory B cells	7
A FluoroSpot B assay for the detection of IgA and IgG SARS-CoV-2 spike-specific memory B cells: Optimization and qualification for use in COVID-19 vaccine trials	7
Comparison of methods to isolate peripheral blood mononuclear cells from cattle blood	7
Comparison of single-chain variable fragments and monoclonal antibody against dihydroartemisinin	7
Development of an ELISA for an effective potency determination of recombinant rabies human monoclonal antibody	7
Non-invasive image-based cytometry for high throughput NK cell cytolysis analysis	6
Development of a rapid lateral flow immunochromatography assay for the detection of group-specific antibodies against VP7 protein of bluetongue virus in multiple species	6
Stabilization of leukocytes from cerebrospinal fluid for central immunophenotypic evaluation in multicenter clinical trials	6
Editorial – Immunobiophysics: Advances and techniques	6
Development of a new automated IL-6 immunoassay	6
Sensitive amplified luminescent proximity homogeneous assay for the quantitative detection of CA242	6
Small p53 derived peptide suitable for robust nanobodies dimerization	6
Candidate antibody reference reagents for Chlamydia trachomatis serology	6
Agreement and error of titration assays	6
30-color full spectrum flow cytometry panel for deep immunophenotyping of T cell subsets in murine tumor tissue	6
A sensitive and drug tolerant assay for detecting anti-AAV9 antibodies using affinity capture elution	6
Overview of human B-cell development and antibody deficiencies	6

Isolation of phage-antibodies against Eimeria species that infect chickens	6
Editorial Board	5
Development of optimized cytotoxicity assays for assessing the antitumor potential of CAR-T cells	5
Rapid identification of tumor-reactive T-cell receptors by RNA preamplification-based single-cell sequencing	5
Validation of a combined ELISA to detect IgG, IgA and IgM antibody responses to SARS-CoV-2 in mild or moderate non-hospitalised patients	5
Editorial Board	5
Optimization of IgG1 immune complexes to stimulate cytokine production in innate cells	5
Identification of the linear Fc-binding epitope on the bovine IgG1 Fc receptor of (boFcγRI) using synthetic peptides	5
Artificial intelligence and deep learning to map immune cell types in inflamed human tissue	5
Sources of variability in Luminex bead-based cytokine assays: Evidence from twelve years of multi-site proficiency testing	5
Techniques for evaluating the ATP-gated ion channel P2X7 receptor function in macrophages and microglial cells	5
Flow cytometry for B-cell subset analysis in immunodeficiencies	5
Optimisation and validation of a new method for antibody dependent cellular phagocytosis in hepatitis C virus infection	5
Ultrasensitive multiplexed chemiluminescent enzyme-linked immunosorbent assays in 384-well plates	5
Smartphone based lateral flow immunoassay quantifications	5
Advanced hybridoma technology for selective production of high-affinity monoclonal antibodies through B-cell receptors	5
Complete primer set for amplification and expression of full-length recombinant human monoclonal antibodies from single human B cells	5
Rapid, sensitive and cost-effective determination of immune checkpoint inhibitor activity using a magnetic bead-based binding assay	5
Optimized flow cytometry protocol for dihydrorhodamine 123-based detection of reactive oxygen species in leukocyte subpopulations in whole blood	5
Comparison of hemagglutination inhibition tests, immunoperoxidase monolayer assays, and serum neutralizing tests in detecting antibodies against blue eye disease in pigs	5
Editorial Board	5
Epitope profiling of cow's milk allergen-specific antibodies with determining IgE content in epitopes-ALL, a 14-epitopes mixture	5
Comprehensive phenotyping of circulating immune cell subsets in people living with HIV	5
Development of rELISA using novel markers for the diagnosis of paratuberculosis	5
Evaluation of a multiplexed coronavirus antigen array for detection of SARS-CoV-2 specific IgG in COVID-19 convalescent plasma	5
Editorial Board	5
Plaque reduction neutralization test for smallpox vaccines: Laboratory optimization and validation method for immunogenicity assessment	5
An easy assay to detect autoantibodies neutralizing cytokines in subjects with critical infections	5
Development of ELISA for the detection of antibodies against VP2 protein of bluetongue virus serotype-1	5
SARS-CoV-2 serology testing: Progress and challenges	5
Mapping antibody binding using multiplexed epitope substitution analysis	5
Editorial Board	4
Reply to “Precision-cut human liver slice cultures as an immunological platform”	4
ERAMER: A novel in silico tool for prediction of ERAP1 enzyme trimming	4
Evaluation of antibody titer kinetics and SARS-CoV-2 infections in a large cohort of healthcare professionals ten months after administration of the BNT162b2 vaccine	4
A novel whole blood assay to quantify the release of T cell associated cytokines in response to Bordetella pertussis antigens	4
A novel antigen immunochromatography fluorometric strip for rapid detection and application of pathogenic bacterial high-quality antibody	4
MILKSHAKE Western blot and Sundae ELISA: We all scream for better antibody validation	4
Development and evaluation of a multi-antigen serological assay for the intra-vitam diagnosis of Tuberculosis caused by Mycobacterium bovis in pigs	4
Retention of E-selectin functionalized liposome fanny packs on Jurkat cells following invasion through collagen	4
Individual polysaccharide quantification in polyvalent pneumococcal conjugate vaccine using rate nephelometry	4
Stability of anti-drug antibodies in human serum samples	4
Generation of good manufacturing practice grade virus-specific T cells for the management of post-transplant CMV infections	4
Highlights of the 33rd annual scientific meeting of the Association of Medical Laboratory Immunologists (AMLI)	4
Methyl ester and aromatic ether modification of mitragynine for generation of mitragynine-specific polyclonal antibodies	4
Novel immunoassay for diagnosis of ongoing Clostridioides difficile infections using serum and medium enriched for newly synthesized antibodies (MENSA)	4
An eight-plex immunoassay for Group A streptococcus serology and vaccine development	4
Editorial – Special Issue on in vitro detection of drug allergy	4
Isolation of anti-Ancylostoma-secreted protein 5 (ASP5) antibody from a naïve antibody phage library	4
Editorial Board	4
Parameters of TNF receptor co-expression in allergic and autoimmune processes: Differences and diagnostic significance	4
Establishment of bioluminescent imaging model using murine T cell lymphoma susceptive to NK cell-dependent immune-surveillance	4
Whole blood based point-of-care assay for the detection of anti-pertussis toxin IgG antibodies	4
Detection of RNA-dependent RNA polymerase of porcine epidemic diarrhea virus	4
Fresh versus frozen PMBC using the SARS CoV-2 T-SPOT.COVID test. Which works best?	4
A novel immuno-molecular strategy for the detection of Streptococcus pneumoniae serotypes in human cerebrospinal and middle ear fluids	4
Immunoinformatics and Pepscan strategies on the path of a peptide-based serological diagnosis of COVID19	4
Diagnosis of septic shock by serum measurement of human neutrophil lipocalin by a rapid homogeneous assay	4
The role of gamma globulin, complement component 1q, factor B, properdin, body temperature, C-reactive protein and serum amyloid alpha to the activity and the function of the human complement system a	4
Comparative assessment of cytometry by time-of-flight and full spectral flow cytometry based on a 33-color antibody panel	4
Evaluation of circulating leucocyte populations both in subjects with previous SARS-COV-2 infection and in healthy subjects after vaccination	3
In vitro differentiation of myeloid suppressor cells (MDSC-like) from an immature myelomonocytic precursor THP-1	3
Development and validation of indirect ELISA for antibody detection against different protein antigens of porcine epidemic diarrhea virus in the colostrum and milk of sows	3
Rapid, sensitive, and specific lateral-flow immunochromatographic point-of-care device for detection of varicella-zoster virus immunoglobulin G antibodies in fingerstick blood	3
Identification of polyreactive antibodies by high throughput enzyme-linked immunosorbent assay and surface Plasmon resonance	3
Comparison of two anti-histones antibodies commercial assays in an immunology laboratory and systemic lupus erythematosus	3
Bacterial coloration immunofluorescence strip for ultrasensitive rapid detection of bacterial antibodies and targeted antibody-secreting hybridomas	3
Letter to the editor regarding “Stabilization of leukocytes from cerebrospinal fluid for central immunophenotypic evaluation in multicenter clinical trials”	3
High-yield production of recombinant human myelin oligodendrocyte glycoprotein in SHuffle bacteria without a refolding step	3
Proficiency tests to evaluate the impact on assay outcomes of harmonized influenza-specific Intracellular Cytokine Staining (ICS) and IFN-ɣ Enzyme-Linked ImmunoSpot (ELISpot) protocols	3
In vitro immunization approach to generate specific murine monoclonal IgG antibodies	3
Validation of dot blot immunoassay for measurement of complement opsonization of nanoparticles	3
Development and qualification of a fast, high-throughput and robust imaging-based neutralization assay for respiratory syncytial virus	3
The C-terminus of Brucella abortus MviN induces humoral and cell mediated immune responses in BALB/c mice that protects against the virulent Brucella 544 challenge	3
Potential diagnostic application of the baculovirus-expressed recombinant truncated nucleocapsid protein of peste des petits ruminants virus in ELISA	3
Highly versatile antibody binding assay for the detection of SARS-CoV-2 infection and vaccination	3
Detection of alloreactive T cells from cryopreserved human peripheral blood mononuclear cells	3
Flow cytometric determination of ferroptosis using a rat monoclonal antibody raised against ferroptotic cells	3
Optimisation of SARS-CoV-2 peptide stimulation and measurement of cytokine output by intracellular flow cytometry and bio-plex analysis	3
Flow cytometry reveals constant lymphocyte proportions after long-term cryopreservation of whole blood in TransFix® cell stabilization reagent	3

Novel hapten design, highly sensitive monoclonal antibody production, and immunoassay development for rapid screening of illegally added chloramphenicol in cosmetics	3
Humoral and cellular response of two different vaccines against SARS-CoV-2 in a group of healthcare workers: Comment	3
Monocyte monolayer assay in pre-transfusion testing: A magic key in transfusing patients with recurrent bad cross-match due to alloimmunization	3
Comparative analysis of LAG3 antibodies shows differential binding patterns by flow cytometry	3
Neuronal binding by antibodies can be influenced by low pH stress during the isolation procedure	3
Fine definition of the epitopes on the human gp91 /NOX2 for the monoclonal antibodies CL-5 and 48	3
Surface Plasmon Resonance (SPR) biosensor for detection of mycotoxins: A review	3
Functional assays for the diagnosis of chronic granulomatous disease	3
Generation of an alpaca serum that induces immune-mediated crescentic glomerulonephritis in mice	3
Electrofusion preparation of anti-triazophos monoclonal antibodies for development of an indirect competitive enzyme-linked immunosorbent assay	3
Human monoclonal antibody cloning and expression with overlap extension PCR and short DNA fragments	3
Development of a non-biased, high-throughput ELISA for the rapid evaluation of immunogenicity and cross-reactivity	3
Dried blood spots are a valid alternative to venipuncture for COVID-19 antibody testing	3
Simultaneous determination of inflammatory factors SAA and LTF based on stable element labeling and inductively coupled plasma mass spectrometry to aid in the diagnosis of infection	3
Assessing TCR identity, knock-in efficiency, and potency for individualized TCR-T cell therapy	3
Immunofluorescence analysis of human eosinophils	3
Validation of dried blood spots samples for the screening of hepatitis B virus surface by enzyme immunoassay method	3
“Common variable immunodeficiency: Challenges for diagnosis”	3
Mitochondrial function in oral health and disease	3
Development and evaluation of a low cost IgG ELISA test based in RBD protein for COVID-19	3
Tumor nano-lysate activates dendritic cells to evoke a preventative immune response	3
Effect of wash media type during PBMC isolation on downstream characterization of SARS-CoV-2-specific T cells	3
Development of a light-initiated chemiluminescence assay for the quantitative detection of cow's milk allergen-specific IgE	3
Dissociation of murine oral mucosal tissues for single cell applications	3