Laboratory Investigation

Article	Citations
Artificial intelligence and computational pathology	233
PI3K-Akt-mTOR/PFKFB3 pathway mediated lung fibroblast aerobic glycolysis and collagen synthesis in lipopolysaccharide-induced pulmonary fibrosis	106
Grading of adult diffuse gliomas according to the 2021 WHO Classification of Tumors of the Central Nervous System	77
High-throughput proteomics: a methodological mini-review	76
Detection of SARS-CoV-2 in formalin-fixed paraffin-embedded tissue sections using commercially available reagents	73
Retrospective analysis of laboratory testing in 54 patients with severe- or critical-type 2019 novel coronavirus pneumonia	69
Adipose mesenchymal stem cell-derived exosomes accelerate skin wound healing via the lncRNA H19/miR-19b/SOX9 axis	58
Quantitative measurement of HER2 expression to subclassify ERBB2 unamplified breast cancer	54
Multiplexed ion beam imaging (MIBI) for characterization of the tumor microenvironment across tumor types	50
CCL3–CCR5 axis contributes to progression of esophageal squamous cell carcinoma by promoting cell migration and invasion via Akt and ERK pathways	45
N-methyladenosine reader YTHDF2-mediated long noncoding RNA FENDRR degradation promotes cell proliferation in endometrioid endometrial carcinoma	44
Deep learning shows the capability of high-level computer-aided diagnosis in malignant lymphoma	40
Vascular remodeling by placenta-derived mesenchymal stem cells restores ovarian function in ovariectomized rat model via the VEGF pathway	38
Deep-learning-based accurate hepatic steatosis quantification for histological assessment of liver biopsies	37
Advancements in detection of SARS-CoV-2 infection for confronting COVID-19 pandemics	36
PAI-1 derived from cancer-associated fibroblasts in esophageal squamous cell carcinoma promotes the invasion of cancer cells and the migration of macrophages	33
Exosomal tau with seeding activity is released from Alzheimer's disease synapses, and seeding potential is associated with amyloid beta	32
Wolfram syndrome 1 gene regulates pathways maintaining beta-cell health and survival	32
The lncRNA XIST/miR-150-5p/c-Fos axis regulates sepsis-induced myocardial injury via TXNIP-modulated pyroptosis	32
Th17/IL-17 induces endothelial cell senescence via activation of NF-κB/p53/Rb signaling pathway	31
Fibronectin 1 activates WNT/β-catenin signaling to induce osteogenic differentiation via integrin β1 interaction	31
miR-27a ameliorates chemoresistance of breast cancer cells by disruption of reactive oxygen species homeostasis and impairment of autophagy	31
MicroRNA-182 improves spinal cord injury in mice by modulating apoptosis and the inflammatory response via IKKβ/NF-κB	31
CircRNA-ST6GALNAC6 increases the sensitivity of bladder cancer cells to erastin-induced ferroptosis by regulating the HSPB1/P38 axis	30
Nicotinamide riboside, an NAD+ precursor, attenuates inflammation and oxidative stress by activating sirtuin 1 in alcohol-stimulated macrophages	30

Brightfield multiplex immunohistochemistry with multispectral imaging	30
3′ MACE RNA-sequencing allows for transcriptome profiling in human tissue samples after long-term storage	29
Predictive modeling of estrogen receptor agonism, antagonism, and binding activities using machine- and deep-learning approaches	29
Establishing a living biobank of patient-derived organoids of intraductal papillary mucinous neoplasms of the pancreas	28
Hemoglobin oxidation generates globin-derived peptides in atherosclerotic lesions and intraventricular hemorrhage of the brain, provoking endothelial dysfunction	28
Exosomes derived from hypoxic glioma deliver miR-1246 and miR-10b-5p to normoxic glioma cells to promote migration and invasion	28
Mitochondrial dysfunction/NLRP3 inflammasome axis contributes to angiotensin II-induced skeletal muscle wasting via PPAR-γ	28
LncRNA GAS5 activates the HIF1A/VEGF pathway by binding to TAF15 to promote wound healing in diabetic foot ulcers	27
Mechanical strain induces phenotypic changes in breast cancer cells and promotes immunosuppression in the tumor microenvironment	27
M1 macrophage-derived exosomes transfer miR-222 to induce bone marrow mesenchymal stem cell apoptosis	27
Exercise suppresses NLRP3 inflammasome activation in mice with diet-induced NASH: a plausible role of adropin	27
TGFβ1-Smad canonical and -Erk noncanonical pathways participate in interleukin-17-induced epithelial–mesenchymal transition in Sjögren's syndrome	27
LncRNA XIST shuttled by adipose tissue-derived mesenchymal stem cell-derived extracellular vesicles suppresses myocardial pyroptosis in atrial fibrillation by disrupting miR-214-3p-mediated Arl2 inhib	26
CircPAN3 ameliorates myocardial ischaemia/reperfusion injury by targeting miR-421/Pink1 axis-mediated autophagy suppression	26
ECM1 secreted by HER2-overexpressing breast cancer cells promotes formation of a vascular niche accelerating cancer cell migration and invasion	26
Human urine-derived stem cell-derived exosomal miR-21-5p promotes neurogenesis to attenuate Rett syndrome via the EPha4/TEK axis	26
Myocardial tissue-specific Dnmt1 knockout in rats protects against pathological injury induced by Adriamycin	26
Adipophilin expression in cutaneous malignant melanoma is associated with high proliferation and poor clinical prognosis	25
SPOCK1 overexpression induced by platelet-derived growth factor-BB promotes hepatic stellate cell activation and liver fibrosis through the integrin α5β1/PI3K/Akt signaling pathway	25
Quantitative assessment of inflammatory infiltrates in kidney transplant biopsies using multiplex tyramide signal amplification and deep learning	25
Digital quantitative assessment of PD-L1 using digital spatial profiling	25
Matrix metalloproteinase 11 (MMP11) in macrophages promotes the migration of HER2-positive breast cancer cells and monocyte recruitment through CCL2–CCR2 signaling	25
Curcumin attenuates Adriamycin-resistance of acute myeloid leukemia by inhibiting the lncRNA HOTAIR/miR-20a-5p/WT1 axis	25
Enhanced alcoholic liver disease in mice with intestine-specific farnesoid X receptor deficiency	24
Kras mutation rate precisely orchestrates ductal derived pancreatic intraepithelial neoplasia and pancreatic cancer	24
Pseudomonas aeruginosa virulence proteins pseudolysin and protease IV impede cutaneous wound healing	24
Deep learning-based grading of ductal carcinoma in situ in breast histopathology images	23
CD38 in cancer-associated fibroblasts promotes pro-tumoral activity	23
MiR-221-3p targets Hif-1α to inhibit angiogenesis in heart failure	22
Angiotensin-converting enzyme 2 influences pancreatic and renal function in diabetic mice	21
Extracellular vesicles derived from M2 microglia reduce ischemic brain injury through microRNA-135a-5p/TXNIP/NLRP3 axis	21
Sporadic and Lynch syndrome-associated mismatch repair-deficient brain tumors	21
MiR-185-5p ameliorates endoplasmic reticulum stress and renal fibrosis by downregulation of ATF6	21
Large-scale, three-dimensional tissue cytometry of the human kidney: a complete and accessible pipeline	21
Inhibition of miR-181a-5p reduces astrocyte and microglia activation and oxidative stress by activating SIRT1 in immature rats with epilepsy	21
LncRNA MAPKAPK5_AS1 facilitates cell proliferation in hepatitis B virus -related hepatocellular carcinoma	21
Blocking thrombospondin-1 signaling via CD47 mitigates renal interstitial fibrosis	21
LncRNA SLCO4A1-AS1 modulates colon cancer stem cell properties by binding to miR-150-3p and positively regulating SLCO4A1	21
Distinct macrophage polarization in acute and chronic gout	20
S100A4/non-muscle myosin II signaling regulates epithelial-mesenchymal transition and stemness in uterine carcinosarcoma	20
Characterization of pathological thyroid tissue using polarization-sensitive second harmonic generation microscopy	20
Identification of differentially expressed genes in lung adenocarcinoma cells using single-cell RNA sequencing not detected using traditional RNA sequencing and microarray	20
Characterization of COVID-19-associated cardiac injury: evidence for a multifactorial disease in an autopsy cohort	20
PCTR1 ameliorates lipopolysaccharide-induced acute inflammation and multiple organ damage via regulation of linoleic acid metabolism by promoting FADS1/FASDS2/ELOV2 expression and reducing PLA2 expres	20
Molecular signatures of tumor progression in myxoid liposarcoma identified by N-glycan mass spectrometry imaging	20
The HNRNPA2B1–MST1R–Akt axis contributes to epithelial-to-mesenchymal transition in head and neck cancer	20
Zyxin (ZYX) promotes invasion and acts as a biomarker for aggressive phenotypes of human glioblastoma multiforme	20
GestAltNet: aggregation and attention to improve deep learning of gestational age from placental whole-slide images	20
Extracellular cold-inducible RNA-binding protein regulates neutrophil extracellular trap formation and tissue damage in acute pancreatitis	20
Performance and efficiency of machine learning algorithms for analyzing rectangular biomedical data	20

Urinary ERdj3 and mesencephalic astrocyte-derived neutrophic factor identify endoplasmic reticulum stress in glomerular disease	20
The antioxidative stress regulator Nrf2 potentiates radioresistance of oral squamous cell carcinoma accompanied with metabolic modulation	19
A convolutional neural network model for survival prediction based on prognosis-related cascaded Wx feature selection	19
Bioglass promotes wound healing by inhibiting endothelial cell pyroptosis through regulation of the connexin 43/reactive oxygen species (ROS) signaling pathway	19
Hepatocyte-specific fibroblast growth factor 21 overexpression ameliorates high-fat diet-induced obesity and liver steatosis in mice	19
The role of macrophage–fibroblast interaction in lipopolysaccharide-induced pulmonary fibrosis: an acceleration in lung fibroblast aerobic glycolysis	19
FABP4 activates the JAK2/STAT2 pathway via Rap1a in the homocysteine-induced macrophage inflammatory response in ApoE mice atherosclerosis	19
Sustained hedgehog signaling in medulloblastoma tumoroids is attributed to stromal astrocytes and astrocyte-derived extracellular matrix	19
Reproducible, high-dimensional imaging in archival human tissue by multiplexed ion beam imaging by time-of-flight (MIBI-TOF)	18
Overexpression of pigment epithelium-derived factor in placenta-derived mesenchymal stem cells promotes mitochondrial biogenesis in retinal cells	18
Macrophage-derived neurotrophin-3 promotes heterotopic ossification in rats	18
Crosstalk between progesterone receptor membrane component 1 and estrogen receptor α promotes breast cancer cell proliferation	18
Feline low-grade intestinal T cell lymphoma: a unique natural model of human indolent T cell lymphoproliferative disorder of the gastrointestinal tract	17
The synthetic toxin biliatresone causes biliary atresia in mice	17
Accelerated instability testing reveals quantitative mass spectrometry overcomes specimen storage limitations associated with PD-L1 immunohistochemistry	17
Steatosis, inflammasome upregulation, and fibrosis are attenuated in miR-155 deficient mice in a high fat-cholesterol-sugar diet-induced model of NASH	17
Establishing standardized immune phenotyping of metastatic melanoma by digital pathology	17
A mouse model for peritoneal metastases of colorectal origin recapitulates patient heterogeneity	17
MicroRNA-130b functions as an oncogene and is a predictive marker of poor prognosis in lung adenocarcinoma	17
Biliary damage and liver fibrosis are ameliorated in a novel mouse model lacking l-histidine decarboxylase/histamine signaling	17
Ubiquitin-specific protease 35 (USP35) mediates cisplatin-induced apoptosis by stabilizing BIRC3 in non-small cell lung cancer	16
Phospholipase Cγ1 (PLCG1) overexpression is associated with tumor growth and poor survival in IDH wild-type lower-grade gliomas in adult patients	16
GTSE1 promotes prostate cancer cell proliferation via the SP1/FOXM1 signaling pathway	16
IL-37bΔ1-45 suppresses the migration and invasion of endometrial cancer cells by targeting the Rac1/NF-κB/MMP2 signal pathway	16
SIRT6 regulates SREBP1c-induced glucolipid metabolism in liver and pancreas via the AMPKα-mTORC1 pathway	16
Impairment of corneal epithelial wound healing is association with increased neutrophil infiltration and reactive oxygen species activation in tenascin X-deficient mice	16
SECISBP2 is a novel prognostic predictor that regulates selenoproteins in diffuse large B-cell lymphoma	16
Sox13 promotes hepatocellular carcinoma metastasis by transcriptionally activating Twist1	15
NFAT5 promotes oral squamous cell carcinoma progression in a hyperosmotic environment	15
Altered glycosylation of glycodelin in endometrial carcinoma	15
LncRNA SNHG5 upregulation induced by YY1 contributes to angiogenesis via miR-26b/CTGF/VEGFA axis in acute myelogenous leukemia	15
DNA methyltransferase 1 (DNMT1) suppresses mitophagy and aggravates heart failure via the microRNA-152-3p/ETS1/RhoH axis	15
MiR-126-5p promotes contractile switching of aortic smooth muscle cells by targeting VEPH1 and alleviates Ang II-induced abdominal aortic aneurysm in mice	15
miR-184 targets TP63 to block idiopathic pulmonary fibrosis by inhibiting proliferation and epithelial–mesenchymal transition of airway epithelial cells	15
βCysteine 93 in human hemoglobin: a gateway to oxidative stability in health and disease	15
Aryl Hydrocarbon Receptor Activation Coordinates Mouse Small Intestinal Epithelial Cell Programming	15
Adipose tissue inflammation and systemic insulin resistance in mice with diet-induced obesity is possibly associated with disruption of PFKFB3 in hematopoietic cells	15
A novel image signature-based radiomics method to achieve precise diagnosis and prognostic stratification of gliomas	15
RAMP1 signaling in immune cells regulates inflammation-associated lymphangiogenesis	14
Regulation of pancreatic cancer TRAIL resistance by protein O-GlcNAcylation	14
Loss of ephrin B2 receptor (EPHB2) sets lipid rheostat by regulating proteins DGAT1 and ATGL inducing lipid droplet storage in prostate cancer cells	14
6-Gingerol relieves myocardial ischaemia/reperfusion injury by regulating lncRNA H19/miR-143/ATG7 signaling axis-mediated autophagy	14
Nuclear exclusion of YAP exacerbates podocyte apoptosis and disease progression in Adriamycin-induced focal segmental glomerulosclerosis	14
SMAD4 mutation correlates with poor prognosis in non-small cell lung cancer	14
Alternative activation of macrophages by prostacyclin synthase ameliorates alcohol induced liver injury	14
Pretreatment with valproic acid alleviates pulmonary fibrosis through epithelial–mesenchymal transition inhibition in vitro and in vivo	14
Reliability of liquid biopsy analysis: an inter-laboratory comparison of circulating tumor DNA extraction and sequencing with different platforms	14
Parthenolide alleviates peritoneal fibrosis by inhibiting inflammation via the NF-κB/ TGF-β/Smad signaling axis	14
MiR-200c-3p targets SESN1 and represses the IL-6/AKT loop to prevent cholangiocyte activation and cholestatic liver fibrosis	13
Standardized Pathology Screening of Mature Tertiary Lymphoid Structures in Cancers	13
A simplified integrated molecular and immunohistochemistry-based algorithm allows high accuracy prediction of glioblastoma transcriptional subtypes	13
Mechanical loading activates the YAP/TAZ pathway and chemokine expression in the MLO-Y4 osteocyte-like cell line	13
Exploring photoacoustic spectroscopy-based machine learning together with metabolomics to assess breast tumor progression in a xenograft model ex vivo	13
Cholestasis impairs hepatic lipid storage via AMPK and CREB signaling in hepatitis B virus surface protein transgenic mice	12
Silencing of LINC01963 enhances the chemosensitivity of prostate cancer cells to docetaxel by targeting the miR-216b-5p/TrkB axis	12
Hepatitis B virus core protein promotes the expression of neuraminidase 1 to facilitate hepatocarcinogenesis	12
MicroRNA-432-5p regulates sprouting and intussusceptive angiogenesis in osteosarcoma microenvironment by targeting PDGFB	12
CircN4BP2L2 promotes colorectal cancer growth and metastasis through regulation of the miR-340-5p/CXCR4 axis	12
EZH2 is associated with cartilage degeneration in osteoarthritis by promoting SDC1 expression via histone methylation of the microRNA-138 promoter	12
Deepfake Histologic Images for Enhancing Digital Pathology	12
TRIM27 contributes to glomerular endothelial cell injury in lupus nephritis by mediating the FoxO1 signaling pathway	12
Circulating tumor DNA profiling for childhood brain tumors: Technical challenges and evidence for utility	12
Early pathogenesis of cystic fibrosis gallbladder disease in a porcine model	11
Distinct transcriptional programs of SOX2 in different types of small cell lung cancers	11
LncRNA UCC promotes epithelial–mesenchymal transition via the miR-143-3p/SOX5 axis in non-small-cell lung cancer	11
PLODs are overexpressed in ovarian cancer and are associated with gap junctions via connexin 43	11
Long noncoding Mirt2 reduces apoptosis to alleviate myocardial infarction through regulation of the miR-764/PDK1 axis	11
Monomeric C reactive protein (mCRP) regulates inflammatory responses in human and mouse chondrocytes	11
p53 Activates the Lipoxygenase Activity of ALOX15B via Inhibiting SLC7A11 to Induce Ferroptosis in Bladder Cancer Cells	11
The transient receptor potential vanilloid 4 (TRPV4) ion channel mediates protease activated receptor 1 (PAR1)-induced vascular hyperpermeability	11
A MXI1-NUTM1 fusion protein with MYC-like activity suggests a novel oncogenic mechanism in a subset of NUTM1-rearranged tumors	11
Long non-coding RNA MALAT1 enhances angiogenesis during bone regeneration by regulating the miR-494/SP1 axis	11
Generation of a new immortalized human lung pericyte cell line: a promising tool for human lung pericyte studies	11
Predicting 1p/19q co-deletion status from magnetic resonance imaging using deep learning in adult-type diffuse lower-grade gliomas: a discovery and validation study	11
LncRNA SNHG15 relieves hyperglycemia-induced endothelial dysfunction via increased ubiquitination of thioredoxin-interacting protein	11
Generation of heterozygous PKD1 mutant pigs exhibiting early-onset renal cyst formation	11
Analysis of DNA variants in miRNAs and miRNA 3ʼUTR binding sites in female infertility patients	10
Inhibitory effects of temozolomide on glioma cells is sensitized by RSL3-induced ferroptosis but negatively correlated with expression of ferritin heavy chain 1 and ferritin light chain	10
αTAT1-induced tubulin acetylation promotes ameloblastoma migration and invasion	10
Ascorbate-induced oxidative stress mediates TRP channel activation and cytotoxicity in human etoposide-sensitive and -resistant retinoblastoma cells	10

Vasoinhibin reduces joint inflammation, bone loss, and the angiogenesis and vasopermeability of the pannus in murine antigen-induced arthritis	10
Inhibition of syndecan-4 reduces cartilage degradation in murine models of osteoarthritis through the downregulation of HIF-2α by miR-96-5p	10
Pigs with δ-sarcoglycan deficiency exhibit traits of genetic cardiomyopathy	10
Bone morphogenetic protein induces bone invasion of melanoma by epithelial–mesenchymal transition via the Smad1/5 signaling pathway	10
lncRNA NEAT1 aggravates sepsis-induced lung injury by regulating the miR-27a/PTEN axis	10
LncRNA ROR modulates myocardial ischemia-reperfusion injury mediated by the miR-185-5p/CDK6 axis	10
Anti-tumor effects of vitamin D in glioblastoma: mechanism and therapeutic implications	10
Resveratrol promotes skin wound healing by regulating the miR-212/CASP8 axis	10
Early response in phosphorylation of ribosomal protein S6 is associated with sensitivity to trametinib in colorectal cancer cells	10
A new technological approach in diagnostic pathology: mass spectrometry imaging-based metabolomics for biomarker detection in urachal cancer	10
Exosomes derived from hypoxia-induced alveolar epithelial cells stimulate interstitial pulmonary fibrosis through a HOTAIRM1-dependent mechanism	10
Morphological changes in synovial mesenchymal stem cells during their adhesion to the meniscus	10
LINC01419-mediated epigenetic silencing of ZIC1 promotes metastasis in hepatocellular carcinoma through the PI3K/Akt signaling pathway	10
Genetic loss-of-function of activating transcription factor 3 but not C-type lectin member 5A prevents diabetic peripheral neuropathy	10
A generative adversarial approach to facilitate archival-quality histopathologic diagnoses from frozen tissue sections	10
Novel role of the dietary flavonoid fisetin in suppressing rRNA biogenesis	9
circHECTD1 attenuates apoptosis of alveolar epithelial cells in acute lung injury	9
ZKSCAN3 in severe bacterial lung infection and sepsis-induced immunosuppression	9
Induction of cancer cell stemness in glioma through glycolysis and the long noncoding RNA HULC-activated FOXM1/AGR2/HIF-1α axis	9
GTSE1 promotes tumor growth and metastasis by attenuating of KLF4 expression in clear cell renal cell carcinoma	9
Dissection of PD-L1 promoter reveals differential transcriptional regulation of PD-L1 in VHL mutant clear cell renal cell carcinoma	9
Interpretable tumor differentiation grade and microsatellite instability recognition in gastric cancer using deep learning	9
Characterization of colorectal mucus using infrared spectroscopy: a potential target for bowel cancer screening and diagnosis	9
Commensal gut bacterium critically regulates alveolar bone homeostasis	9
Conventional histological and cytological staining with simultaneous immunohistochemistry enabled by invisible chromogens	9
Assessing the utility of long-read nanopore sequencing for rapid and efficient characterization of mobile element insertions	9
Knockdown of sodium channel Nax reduces dermatitis symptoms in rabbit skin	9
Pharmacological inhibition of serine synthesis enhances temozolomide efficacy by decreasing O6-methylguanine DNA methyltransferase (MGMT) expression and reactive oxygen species (ROS)-mediated DNA dama	9
Protein-based immune profiles of basal-like vs. luminal breast cancers	9
Amelioration of circadian disruption and calcium-handling protein defects by choline alleviates cardiac remodeling in abdominal aorta coarctation rats	9
A novel human endometrial epithelial cell line for modeling gynecological diseases and for drug screening	9
CP-25 alleviates antigen-induced experimental Sjögren's syndrome in mice by inhibiting JAK1-STAT1/2-CXCL13 signaling and interfering with B-cell migration	9
A time-course Raman spectroscopic analysis of spontaneous in vitro microcalcifications in a breast cancer cell line	9
Osr1 regulates hepatic inflammation and cell survival in the progression of non-alcoholic fatty liver disease	9
MicroRNA-483-5p accentuates cisplatin-induced acute kidney injury by targeting GPX3	9
Reversal of hypertriglyceridemia in diabetic BTBR ob/ob mice does not prevent nephropathy	9
AccuCor2: isotope natural abundance correction for dual-isotope tracer experiments	9