OOIR: Observatory of International Research

Papers

(The TQCC of Enzyme and Microbial Technology is 9. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-11-01 to 2025-11-01.)

Article	Citations
Microbial production of adipic acid from 6-hydroxyhexanoic acid for biocatalytic upcycling of polycaprolactone	175
Osmoregulation by choline-based deep eutectic solvent induces electroactivity in Bacillus subtilis biofilms	106
Characterization of a novel short-chain dehydrogenase from Sugiyamaella lignohabitans and its application in the degradation of patulin	104
Enhancement of thermostability of GH10 xylanase E Penicillium canescens directed by ΔΔG calculations and structure analysis	61
Alcalase immobilization in iota-carrageenan-matrix hydrogel beads derived from the macroalga Solieria filiformis	42
Molecular cloning and characterization of a novel bifunctional cellobiohydrolase/β-xylosidase from a metagenomic library of mangrove soil	38
Carbohydrate-active enzyme-catalyzed stereoselective glycosylation of complex natural product glycosides	34
Analysis of enzyme kinetics of fungal methionine synthases in an optimized colorimetric microscale assay for measuring cobalamin-independent methionine synthase activity	33
Biochar production from microalgae: a new sustainable approach to wastewater treatment based on a circular economy	33
Microbial engineering for shikimate biosynthesis	33
Latent potentials of the white-rot basidiomycete Phanerochaete chrysosporium responsible for sesquiterpene metabolism: CYP5158A1 and CYP5144C8 decorate (E)-α-bisabolene	31
More efficient barley malting under catalyst: Thermostability improvement of a β-1,3-1,4-glucanase through surface charge engineering with higher activity	31
Disrupting putative N-glycosylation site N17 in lipase Lip11 of Yarrowia lipolytica yielded a catalytically efficient and thermostable variant accompanying conformational changes	29
Highly sensitive detection of glucose via glucose oxidase immobilization onto conducting polymer-coated composite polyacrylonitrile nanofibers	29
Metataxonomic characterization of an autochthonous and allochthonous microbial consortium involved in a two-stage anaerobic batch reactor applied to hydrogen and methane production from sugarcane baga	29
Insights on kraft lignin degradation in an anaerobic environment	28
Enhancing the thermal stability and activity of zearalenone lactone hydrolase to promote zearalenone degradation via semi-rational design	27
Identification of a bacteria P450 enzyme from B. megaterium H-1 with vitamin D3 C-25 hydroxylation capabilities	27
Improvement of lipid production from glucose/xylose mixed-sugar by the oleaginous yeast Lipomyces starkeyi through ultra-violet mutagenesis	26
Exploring Helicobacter pylori Histidyl tRNA synthetase as a drug target using biophysical and computational approaches	25
Cloning, purification and characterization of a novel thermostable recombinant tannase from Galactobacillus timonensis	25
Tailoring of levansucrase product size by a comparative molecular dynamics approach	25
Editorial Board	23
Divergent substrate specificities and regioselectivities of three lipase isoforms from Cordyceps militaris: Combinatorial advantages for entomopathogenicity and prospects as biocatalysts	23
Characterization and substrate-accelerated thermal inactivation kinetics of a new serine-type arylsulfatase	23

Structural and functional insights of the catalytic GH5 and Calx-β domains from the metagenome-derived endoglucanase CelE2	23
The potential of R. toruloides mevalonate pathway genes in increasing isoprenoid yields in S. cerevisiae: Evaluation of GGPPS and HMG-CoA reductase	23
Isolation of a newly Trichoderma asperellum LYS1 with abundant cellulase-hemicellulase enzyme cocktail for lignocellulosic biomass degradation	22
Robust cross-linked cyclodextrin glucanotransferase from Bacillus lehensis G1 aggregates using an improved cross-linker and a new co-aggregant for the production of cyclodextrins	22
Development of a novel expression platform for heterologous protein production via deleting the p53-like regulator Vib1 in Trichoderma reesei	21
Editorial Board	21
Improving the cellobiose hydrolysis activity of glucose-stimulating β-glucosidase Bgl2A	21
Nanobodies as binding-chaperones stabilize the recombinant Bombyx mori acetylcholinesterase and protect the enzyme activity in pesticide detection	20
Structures and properties of α-amylase and glucoamylase immobilized by ZIF-8 via one-pot preparation	20
Development and expansion of the CRISPR/Cas9 toolboxes for powerful genome engineering in yeast	20
Synergy of GH67 and GH115 α-1,2-glucuronidases with Penicillium subrubescens endoxylanases to stimulate xylooligosaccharide production	19
Sensitivity of family GH11 Bacillus amyloliquefaciens xylanase A (BaxA) and the T33I mutant to Oryza sativa xylanase inhibitor protein (OsXIP): An experimental and computational study	19
Rational design improves both thermostability and activity of a new D-tagatose 3-epimerase from Kroppenstedtia eburnean to produce D-allulose	19
Design of a synthetic enzyme cascade for the in vitro fixation of formaldehyde to acetoin	18
Editorial Board	18
High surfactant-tolerant β-mannanase isolated from Dynastes hercules larvae excrement, and identification of its hotspot using site-directed mutagenesis and molecular dynamics simulations	18
Mutational analysis of predicted active site residues of an exoglucanase secreted by Xanthomonas oryzae pv. oryzae to determine their role in catalysis and in virulence on rice	18
Editorial Board	18
Efficient production of γ-aminobutyric acid using engineered Escherichia coli whole-cell catalyst	17
Semi-rational engineering of ω-transaminase for enhanced enzymatic activity to 2-ketobutyrate	17
Production of recombinant glycosidases fused with Usp45 and SpaX to avoid the purification and immobilization stages	17
Release of bifidogenic N-glycans from native bovine colostrum proteins by an endo-β-N-acetylglucosaminidase	16
Improving (2S)-naringenin production by exploring native precursor pathways and screening higher-active chalcone synthases from plants rich in flavonoids	16
Mutational and structural analysis of ribose 5-phosphate isomerase B from Acetivibrio thermocellus: relationship between transformation efficiency and substrate binding pocket conformation	16
Simultaneous enhancement of thermostability and catalytic activity of κ-carrageenase from Pseudoalteromonas tetraodonis by rational design	16
Efficient biosynthesis of lacto-N-neotetraose by a novel β-1,4-galactosyltransferase from Aggregatibacter actinomycetemcomitans NUM4039	16
Comparing similar versions of a connecting helix on the structure of Chondroitinase ABC I	16
Improvements in xylose stability and thermalstability of GH39 β-xylosidase from Dictyoglomus thermophilum by site-directed mutagenesis and insights into its xylose tolerance mechanism	16
Periplasmic expression of Pseudomonas fluorescens peroxidase Dyp1B and site-directed mutant Dyp1B enzymes enhances polymeric lignin degradation activity in Pseudomonas putida KT2440	16
Influence of a rumen ciliate-derived xylanase on the gut microbiota composition: A potential enzyme for prebiotic applications	16
Enzyme immobilization technology as a tool to innovate in the production of biofuels: A special review of the Cross-Linked Enzyme Aggregates (CLEAs) strategy	16
Operation parameters and temperature affected sludge microbial metabolisms: An integrated perspective considering extracellular polymeric substances, soluble microbial products, biomass quantities, an	15
Rational design of short-chain dehydrogenase DHDR for efficient synthesis of (S)-equol	15
Characterization and application of active human α2,6-sialyltransferases ST6GalNAc V and ST6GalNAc VI recombined in Escherichia coli	15
Application of reductive amination by heterologously expressed Thermomicrobium roseum L-alanine dehydrogenase to synthesize L-alanine derivatives	15
Corrigendum to “Kinetic and thermodynamic study of laccase cross-linked onto glyoxyl Immobead 150P carrier: Characterization and application for beechwood biografting” [Enzym. Microb. Technol. 150 (20	15
Investigation of biocatalytic production of lactosucrose and fructooligosaccharides using levansucrases and dairy by-products as starting materials	15
Enhancement of rice husks saccharification through hydrolase preparation assisted by lytic polysaccharide monooxygenase	15
Editorial Board	15
Bioanalytical insight into the life of microbial populations: A chemical monitoring of ureolytic bacteria growth	15
Protein engineering of an alkaline protease from Bacillus licheniformis (BLAP) for efficient and specific chiral resolution of the racemic ethyl tetrahydrofuroate	14
A UPLC-based activity assay method for aminoglycoside phosphotransferase 4-Ia (APH4): A Selectable marker of plant transformation	14
Improved thermostability of type I pullulanase from Bacillus thermoliquefaciens by error-prone PCR	14
Using large-scale multi-module NRPS to heterologously prepare highly efficient lipopeptide biosurfactants in recombinant Escherichia coli	14
Construction and characterization of a Myceliophthora thermophila lytic polysaccharide monooxygenase mutant S174C/A93C with improved thermostability	14
Metabolism of phenolic compounds catalyzed by Tomato CYP736A61	14
Nanotechnology based technological development in biofuel production: Current status and future prospects	14
Enzymatic production of chitooligosaccharides with high degree of polymerisations and their potential application to soy sauce preservation	14
Double T-junction microfluidic and conventional dripping systems for Bacillus subtilis immobilization in calcium alginate microparticles for lipase production	14
Targeted gene manipulation of Leloir pathway genes for the constitutive expression of β-galactosidase and its transgalactosylation product galacto-oligosaccharides from Kluyveromyces lactis GG799 and	13

Microbial community analysis of the biofilms of both working and counter electrodes in single-chamber microbial electrolysis cells	13
A simple and green method for direct determination of hydrogen peroxide and hypochlorite in household disinfectants based on personal glucose meter	13
Enzymatic determination of hypoxanthine in fish samples as a freshness indicator using the CUPRAC colorimetric sensor	13
Coriolus versicolor laccase-based inorganic protein hybrid synthesis for application in biomass saccharification to enhance biological production of hydrogen and ethanol	13
Enhanced biotransformation of the minor ginsenosides in red ginseng extract by Penicillium decumbens β-glucosidase	13
Action pattern of Sulfolobus O-α-glycoligase for synthesis of highly water soluble resveratrol 3,4′-α-diglucoside	13
Improved thermal stability of manganese superoxide dismutase from Staphylococcus equorum through formation of artificial oligomer	13
Metagenomic insight on consortium degradation of soil weathered petroleum and its supplement based on gene abundance change	13
Engineering of ω-transaminase at binding pocket and access tunnel for preparation of (R)-1-phenoxypropan-2-amine	13
Enzymatic lacto-N-biose elongation of human milk oligosaccharides with the GH136 lacto-N-biosidase LnbX engineered for improved transglycosylation	12
Catalytically active inclusion bodies (CatIBs) induced by terminally attached self-assembling coiled-coil domains: To enhance the stability of (R)-hydroxynitrile lyase	12
Agmatine production by Escherichia coli cells expressing SpeA on the extracellular surface	12
Biocatalytic valorization of lignin subunit: Screening a carboxylic acid reductase with high substrate preference to syringyl functional group	12
Identification and characterization of endo-xylanases from families GH10 and GH11 sourced from marine thermal environments	12
Rhamnosyltransferases: Biochemical activities, potential biotechnology for production of natural products and their applications	12
Continuous production of gamma aminobutyric acid by engineered and immobilized Escherichia coli whole-cells in a small-scale reactor system	12
Development of a chloroplast expression system for Dunaliella salina	12
Isolation of new lactobionic acid-producing microorganisms and improvement of their production ability by heterologous expression of glucose dehydrogenase from Pseudomonas taetrolens	12
Mutant β-fructofuranosidase synthesizing blastose [β-d-Fruf-(2→6)-d-Glcp]	12
Biochemical and structural characterization of a family-9 glycoside hydrolase bioprospected from the termite Syntermes wheeleri gut bacteria metagenome	12
Probiotic and functional characterization of newly isolated Lactiplantibacillus plantarum strains from human breast milk and proliferative inhibition potential of metabolites	12
Immobilization of Thermomyces lanuginosus lipase on a new hydrophobic support (Streamline phenyl™): Strategies to improve stability and reusability	12
Editorial Board	12
Penicillin binding proteins-based immunoassay for the selective and quantitative determination of beta-lactam antibiotics	12
Screening β-glucosidase and α-rhamnosidase for biotransformation of naringin to naringenin by the one-pot enzymatic cascade	12
Efficient production of isoquercitin, icariin and icariside II by a novel thermostable α-l-rhamnosidase PodoRha from Paenibacillus odorifer with high α-1, 6-/α-1, 2- glycoside specificity	11
Functional characterization of a GH62 family α-L-arabinofuranosidase from Eupenicillium parvum suitable for monosaccharification of corncob arabinoxylan in combination with key enzymes	11
Whole-cell bioconversion for producing thymoquinone by engineered Saccharomyces cerevisiae	11
Unveiling steps of the TDP degradation pathway in Variovorax paradoxus TBEA6	11
Biotransformation of ferulic acid into vanillyl alcohol and vanillic acid employing thermophilic bacterium Caldimonas thermodepolymerans	11
Editorial Board	11
Editorial Board	11
The increased efficiency of porphyran hydrolysis by constructing a multifunctional enzyme complex from marine microorganisms	11
Editorial Board	11
Biochemical characterisation of glycosylated and deglycosylated forms of phytase from Cronobacter turicensis expressed in Pichia pastoris	11
A new continuous assay for quantitative assessment of enzymatic degradation of poly(ethylene terephthalate) (PET)	10
The effect of the cspA gene on growth development and butenyl-spinosyn biosynthesis in Saccharopolyspora pogona	10
One pot purification and co-immobilization of His-tagged old yellow enzyme and glucose dehydrogenase for asymmetric hydrogenation	10
Disruption of vacuolar protein sorting receptor gene Poxvps10 improves cellulolytic enzyme production by Penicillium oxalicum	10
CO2 capture and lipid production performance of microalgae in the S-shaped photobioreactor under different culture modes	10
Synergistic action of thermophilic pectinases for pectin bioconversion into D-galacturonic acid	10
Nicotinic acid production from 3-methylpyridine by E. coli whole-cell biocatalyst	10
Efficient snailase-based production of mogrol from Luo Han Guo extract in an aqueous-organic system	10
Fungal tyrosinase immobilized on chitosan, calcium alginate, and silica gel for phenol elimination and dye decolorization	10
Co-expression of an isopropanol synthetic operon and eGFP to monitor the robustness of Cupriavidus necator during isopropanol production	10
Semi-rational engineering of 3-Ketosteroid-Δ1-dehydrogenase boosts catalytic efficiency and robustness for steroid bioconversion	10
Non-covalent binding tags for batch and flow biocatalysis	10
Carbohydrate-binding modules of ChiB and ChiC promote the chitinolytic system of Serratia marcescens BWL1001	10
Full-length TprK of Treponema pallidum subsp. pallidum in lipid nanodiscs is a monomeric porin	9
Using directed evolution to improve hydrogen production in chimeric hydrogenases from algal species	9
Identification of 2’-deoxyguanosine for an α-amylase inhibitor in the extracts of the earthworm Eisenia fetida and characterization of its inhibitory activity against porcine pancreatic α-amylase	9
Heterologous expression, purification, and characterization of a recombinant Cordyceps militaris lipase from Candida rugosa-like family in Pichia pastoris	9
Poly(3-hydroxybutyrate) production for food packaging from biomass derived carbohydrates by cupriavidus necator DSM 545	9
Ascorbate oxidase enabling glucometer readout for portable detection of hydrogen peroxide	9
Production of an O-desmethylated product, a major human metabolite, of rabeprazole sulfide by bacterial P450 enzymes	9
Unraveling the role of E. coli and calf intestinal alkaline phosphatase in calcium phosphate synthesis	9
Editorial Board	9
Ancestral sequence reconstruction and spatial structure analysis guided alteration of longer-chain substrate catalysis for Thermomicrobium roseum lipase	9
A green pathway for lignin valorization: Enzymatic lignin depolymerization in biocompatible ionic liquids and deep eutectic solvents	9
A study of targeted mutation of l-rhamnose isomerase to improve the conversion efficiency of D-allose	9
Prodigiosin As N-heterocyclic compound: Production optimization, bioactivity evaluation, and in-silico docking against key enzymes related to inflammation, obesity, diabetes, and the insulin signaling	9
A GH19 lysozyme and peptidase from Myoviridae cyanophages lacking the typical holin-endolysin system exhibit lytic activity	9
A lateral flow biosensor based on duplex-specific nuclease and novel isothermal amplification for detection of influenza virus	9
Editorial Board	9
Improvement in salt-tolerance of Aspergillus oryzae γ-glutamyl transpeptidase via protein chimerization with Aspergillus sydowii homolog	9
Identification of carotenoids biosynthesis pathway in Schizochytrium sp. and utilization in astaxanthin biosynthesis	9
Utilization of sweet sorghum juice as a carbon source for enhancement of itaconic acid production in engineered Corynebacterium glutamicum	9
A thermophilic phosphatase from Methanothermobacter marburgensis and its application to in vitro biosynthesis	9
Immobilization on magnetic PVA/SA@Fe3O4 hydrogel beads enhances the activity and stability of neutral protease	9
Biochemical characterization of the β-glucosidase Glu1B from Coptotermes formosanus produced in Pichia pastoris	9
Simultaneous production of linear α-olefins and 2,5-furandicarboxylic acid by combining two recombinant enzymes OleT-ELP and HMFO-ELP	9