OOIR: Observatory of International Research

Papers

(The TQCC of Enzyme and Microbial Technology is 9. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2022-01-01 to 2026-01-01.)

Article	Citations
Osmoregulation by choline-based deep eutectic solvent induces electroactivity in Bacillus subtilis biofilms	182
Molecular cloning and characterization of a novel bifunctional cellobiohydrolase/β-xylosidase from a metagenomic library of mangrove soil	111
Carbohydrate-active enzyme-catalyzed stereoselective glycosylation of complex natural product glycosides	107
Microbial production of adipic acid from 6-hydroxyhexanoic acid for biocatalytic upcycling of polycaprolactone	65
Analysis of enzyme kinetics of fungal methionine synthases in an optimized colorimetric microscale assay for measuring cobalamin-independent methionine synthase activity	39
Characterization of a novel short-chain dehydrogenase from Sugiyamaella lignohabitans and its application in the degradation of patulin	36
Microbial engineering for shikimate biosynthesis	36
Alcalase immobilization in iota-carrageenan-matrix hydrogel beads derived from the macroalga Solieria filiformis	35
Enhancing the thermal stability and activity of zearalenone lactone hydrolase to promote zearalenone degradation via semi-rational design	34
Biochar production from microalgae: a new sustainable approach to wastewater treatment based on a circular economy	33
More efficient barley malting under catalyst: Thermostability improvement of a β-1,3-1,4-glucanase through surface charge engineering with higher activity	32
Metataxonomic characterization of an autochthonous and allochthonous microbial consortium involved in a two-stage anaerobic batch reactor applied to hydrogen and methane production from sugarcane baga	32
Insights on kraft lignin degradation in an anaerobic environment	31
Latent potentials of the white-rot basidiomycete Phanerochaete chrysosporium responsible for sesquiterpene metabolism: CYP5158A1 and CYP5144C8 decorate (E)-α-bisabolene	30
Modulating dioxygenase and hydroperoxide isomerase activities in Burkholderia thailandensis lipoxygenase	28
Identification of a bacteria P450 enzyme from B. megaterium H-1 with vitamin D3 C-25 hydroxylation capabilities	28
Highly sensitive detection of glucose via glucose oxidase immobilization onto conducting polymer-coated composite polyacrylonitrile nanofibers	28
Structures and properties of α-amylase and glucoamylase immobilized by ZIF-8 via one-pot preparation	27
The potential of R. toruloides mevalonate pathway genes in increasing isoprenoid yields in S. cerevisiae: Evaluation of GGPPS and HMG-CoA reductase	26
Exploring Helicobacter pylori Histidyl tRNA synthetase as a drug target using biophysical and computational approaches	26
Improvement of lipid production from glucose/xylose mixed-sugar by the oleaginous yeast Lipomyces starkeyi through ultra-violet mutagenesis	25
Editorial Board	24
Characterization and substrate-accelerated thermal inactivation kinetics of a new serine-type arylsulfatase	24
Robust cross-linked cyclodextrin glucanotransferase from Bacillus lehensis G1 aggregates using an improved cross-linker and a new co-aggregant for the production of cyclodextrins	24
Tailoring of levansucrase product size by a comparative molecular dynamics approach	23

Editorial Board	23
Synergy of GH67 and GH115 α-1,2-glucuronidases with Penicillium subrubescens endoxylanases to stimulate xylooligosaccharide production	23
Divergent substrate specificities and regioselectivities of three lipase isoforms from Cordyceps militaris: Combinatorial advantages for entomopathogenicity and prospects as biocatalysts	23
Sensitivity of family GH11 Bacillus amyloliquefaciens xylanase A (BaxA) and the T33I mutant to Oryza sativa xylanase inhibitor protein (OsXIP): An experimental and computational study	22
Improving the cellobiose hydrolysis activity of glucose-stimulating β-glucosidase Bgl2A	21
Structural and functional insights of the catalytic GH5 and Calx-β domains from the metagenome-derived endoglucanase CelE2	21
Nanobodies as binding-chaperones stabilize the recombinant Bombyx mori acetylcholinesterase and protect the enzyme activity in pesticide detection	21
Development of a novel expression platform for heterologous protein production via deleting the p53-like regulator Vib1 in Trichoderma reesei	21
Isolation of a newly Trichoderma asperellum LYS1 with abundant cellulase-hemicellulase enzyme cocktail for lignocellulosic biomass degradation	20
Editorial Board	20
Cloning, purification and characterization of a novel thermostable recombinant tannase from Galactobacillus timonensis	20
Design of a synthetic enzyme cascade for the in vitro fixation of formaldehyde to acetoin	20
High surfactant-tolerant β-mannanase isolated from Dynastes hercules larvae excrement, and identification of its hotspot using site-directed mutagenesis and molecular dynamics simulations	20
Development and expansion of the CRISPR/Cas9 toolboxes for powerful genome engineering in yeast	20
Editorial Board	20
Mutational analysis of predicted active site residues of an exoglucanase secreted by Xanthomonas oryzae pv. oryzae to determine their role in catalysis and in virulence on rice	19
Production of recombinant glycosidases fused with Usp45 and SpaX to avoid the purification and immobilization stages	19
Semi-rational engineering of ω-transaminase for enhanced enzymatic activity to 2-ketobutyrate	18
Enzyme immobilization technology as a tool to innovate in the production of biofuels: A special review of the Cross-Linked Enzyme Aggregates (CLEAs) strategy	18
Improving (2S)-naringenin production by exploring native precursor pathways and screening higher-active chalcone synthases from plants rich in flavonoids	18
Efficient biosynthesis of lacto-N-neotetraose by a novel β-1,4-galactosyltransferase from Aggregatibacter actinomycetemcomitans NUM4039	17
Influence of a rumen ciliate-derived xylanase on the gut microbiota composition: A potential enzyme for prebiotic applications	17
Efficient production of γ-aminobutyric acid using engineered Escherichia coli whole-cell catalyst	17
Rational design improves both thermostability and activity of a new D-tagatose 3-epimerase from Kroppenstedtia eburnean to produce D-allulose	17
Periplasmic expression of Pseudomonas fluorescens peroxidase Dyp1B and site-directed mutant Dyp1B enzymes enhances polymeric lignin degradation activity in Pseudomonas putida KT2440	17
Release of bifidogenic N-glycans from native bovine colostrum proteins by an endo-β-N-acetylglucosaminidase	17
Simultaneous enhancement of thermostability and catalytic activity of κ-carrageenase from Pseudoalteromonas tetraodonis by rational design	16
Comparing similar versions of a connecting helix on the structure of Chondroitinase ABC I	16
Corrigendum to “Kinetic and thermodynamic study of laccase cross-linked onto glyoxyl Immobead 150P carrier: Characterization and application for beechwood biografting” [Enzym. Microb. Technol. 150 (20	16
Bioanalytical insight into the life of microbial populations: A chemical monitoring of ureolytic bacteria growth	16
Editorial Board	16
Mutational and structural analysis of ribose 5-phosphate isomerase B from Acetivibrio thermocellus: relationship between transformation efficiency and substrate binding pocket conformation	16
Rational design of short-chain dehydrogenase DHDR for efficient synthesis of (S)-equol	16
Application of reductive amination by heterologously expressed Thermomicrobium roseum L-alanine dehydrogenase to synthesize L-alanine derivatives	16
Using large-scale multi-module NRPS to heterologously prepare highly efficient lipopeptide biosurfactants in recombinant Escherichia coli	15
Integrated Bio-Nano Remediation of Produced Water Using Alcanivorax borkumensis and GQDs-Modified ZnO/La₂O₃ Nanocomposites	15
Nanotechnology based technological development in biofuel production: Current status and future prospects	15
Enzymatic production of chitooligosaccharides with high degree of polymerisations and their potential application to soy sauce preservation	15
Investigation of biocatalytic production of lactosucrose and fructooligosaccharides using levansucrases and dairy by-products as starting materials	15
Enhancement of rice husks saccharification through hydrolase preparation assisted by lytic polysaccharide monooxygenase	15
Operation parameters and temperature affected sludge microbial metabolisms: An integrated perspective considering extracellular polymeric substances, soluble microbial products, biomass quantities, an	15
Characterization and application of active human α2,6-sialyltransferases ST6GalNAc V and ST6GalNAc VI recombined in Escherichia coli	15
A UPLC-based activity assay method for aminoglycoside phosphotransferase 4-Ia (APH4): A Selectable marker of plant transformation	14
Improved thermal stability of manganese superoxide dismutase from Staphylococcus equorum through formation of artificial oligomer	14
Dual-acting single-engineered hybrid-architectured promoters enhance and convert expressions into multi-carbon source-regulated systems in Komagataella phaffii	14
Metabolism of phenolic compounds catalyzed by Tomato CYP736A61	14
A simple and green method for direct determination of hydrogen peroxide and hypochlorite in household disinfectants based on personal glucose meter	14
Construction and characterization of a Myceliophthora thermophila lytic polysaccharide monooxygenase mutant S174C/A93C with improved thermostability	14
Double T-junction microfluidic and conventional dripping systems for Bacillus subtilis immobilization in calcium alginate microparticles for lipase production	14
Coriolus versicolor laccase-based inorganic protein hybrid synthesis for application in biomass saccharification to enhance biological production of hydrogen and ethanol	14

Targeted gene manipulation of Leloir pathway genes for the constitutive expression of β-galactosidase and its transgalactosylation product galacto-oligosaccharides from Kluyveromyces lactis GG799 and	14
Microbial community analysis of the biofilms of both working and counter electrodes in single-chamber microbial electrolysis cells	14
Improved thermostability of type I pullulanase from Bacillus thermoliquefaciens by error-prone PCR	14
Enzymatic determination of hypoxanthine in fish samples as a freshness indicator using the CUPRAC colorimetric sensor	13
Enhanced biotransformation of the minor ginsenosides in red ginseng extract by Penicillium decumbens β-glucosidase	13
Isolation of new lactobionic acid-producing microorganisms and improvement of their production ability by heterologous expression of glucose dehydrogenase from Pseudomonas taetrolens	13
NADPH regeneration for efficient biosynthesis of indigo by flavin-containing monooxygenase and formate dehydrogenase	13
RETRACTED: Identification and recombinant expression of a novel defluorinase from Rhodococcus jostii RHA1, for defluorination and biotransformation of the PFAS compound 6:2 fluorotelomer carboxylic ac	13
Editorial Board	13
Metagenomic insight on consortium degradation of soil weathered petroleum and its supplement based on gene abundance change	13
Protein engineering of an alkaline protease from Bacillus licheniformis (BLAP) for efficient and specific chiral resolution of the racemic ethyl tetrahydrofuroate	13
Engineering of ω-transaminase at binding pocket and access tunnel for preparation of (R)-1-phenoxypropan-2-amine	13
Action pattern of Sulfolobus O-α-glycoligase for synthesis of highly water soluble resveratrol 3,4′-α-diglucoside	13
Biocatalytic valorization of lignin subunit: Screening a carboxylic acid reductase with high substrate preference to syringyl functional group	12
Catalytically active inclusion bodies (CatIBs) induced by terminally attached self-assembling coiled-coil domains: To enhance the stability of (R)-hydroxynitrile lyase	12
Identification and characterization of endo-xylanases from families GH10 and GH11 sourced from marine thermal environments	12
Functional characterization of a GH62 family α-L-arabinofuranosidase from Eupenicillium parvum suitable for monosaccharification of corncob arabinoxylan in combination with key enzymes	12
Development of a chloroplast expression system for Dunaliella salina	12
Continuous production of gamma aminobutyric acid by engineered and immobilized Escherichia coli whole-cells in a small-scale reactor system	12
Immobilization of Thermomyces lanuginosus lipase on a new hydrophobic support (Streamline phenyl™): Strategies to improve stability and reusability	12
Screening β-glucosidase and α-rhamnosidase for biotransformation of naringin to naringenin by the one-pot enzymatic cascade	12
The increased efficiency of porphyran hydrolysis by constructing a multifunctional enzyme complex from marine microorganisms	12
Penicillin binding proteins-based immunoassay for the selective and quantitative determination of beta-lactam antibiotics	12
Anti-biofilm efficiency and substrate specificity of recombinantly produced human paraoxonases	12
Probiotic and functional characterization of newly isolated Lactiplantibacillus plantarum strains from human breast milk and proliferative inhibition potential of metabolites	12
Agmatine production by Escherichia coli cells expressing SpeA on the extracellular surface	12
Unveiling steps of the TDP degradation pathway in Variovorax paradoxus TBEA6	12
Metabolic engineering of Bacillus subtilis for enhanced p-Coumaric acid production and antimicrobial applications	11
Rhamnosyltransferases: Biochemical activities, potential biotechnology for production of natural products and their applications	11
Editorial Board	11
Disruption of vacuolar protein sorting receptor gene Poxvps10 improves cellulolytic enzyme production by Penicillium oxalicum	11
Enzymatic lacto-N-biose elongation of human milk oligosaccharides with the GH136 lacto-N-biosidase LnbX engineered for improved transglycosylation	11
Application of natively expressed chitinase as a sustainable fungal bioshield	11
Editorial Board	11
Biochemical characterisation of glycosylated and deglycosylated forms of phytase from Cronobacter turicensis expressed in Pichia pastoris	11
Efficient snailase-based production of mogrol from Luo Han Guo extract in an aqueous-organic system	11
Biotransformation of ferulic acid into vanillyl alcohol and vanillic acid employing thermophilic bacterium Caldimonas thermodepolymerans	11
Editorial Board	11
Efficient production of isoquercitin, icariin and icariside II by a novel thermostable α-l-rhamnosidase PodoRha from Paenibacillus odorifer with high α-1, 6-/α-1, 2- glycoside specificity	10
Biochemical and structural characterization of a family-9 glycoside hydrolase bioprospected from the termite Syntermes wheeleri gut bacteria metagenome	10
The effect of the cspA gene on growth development and butenyl-spinosyn biosynthesis in Saccharopolyspora pogona	10
Nicotinic acid production from 3-methylpyridine by E. coli whole-cell biocatalyst	10
Using directed evolution to improve hydrogen production in chimeric hydrogenases from algal species	10
Mutant β-fructofuranosidase synthesizing blastose [β-d-Fruf-(2→6)-d-Glcp]	10
Carbohydrate-binding modules of ChiB and ChiC promote the chitinolytic system of Serratia marcescens BWL1001	10
Semi-rational engineering of 3-Ketosteroid-Δ1-dehydrogenase boosts catalytic efficiency and robustness for steroid bioconversion	10
Co-expression of an isopropanol synthetic operon and eGFP to monitor the robustness of Cupriavidus necator during isopropanol production	10
Non-covalent binding tags for batch and flow biocatalysis	10
CO2 capture and lipid production performance of microalgae in the S-shaped photobioreactor under different culture modes	10
Whole-cell bioconversion for producing thymoquinone by engineered Saccharomyces cerevisiae	10
Fungal tyrosinase immobilized on chitosan, calcium alginate, and silica gel for phenol elimination and dye decolorization	10
One pot purification and co-immobilization of His-tagged old yellow enzyme and glucose dehydrogenase for asymmetric hydrogenation	10
Full-length TprK of Treponema pallidum subsp. pallidum in lipid nanodiscs is a monomeric porin	9
Production of an O-desmethylated product, a major human metabolite, of rabeprazole sulfide by bacterial P450 enzymes	9
Simultaneous production of linear α-olefins and 2,5-furandicarboxylic acid by combining two recombinant enzymes OleT-ELP and HMFO-ELP	9
Heterologous expression, purification, and characterization of a recombinant Cordyceps militaris lipase from Candida rugosa-like family in Pichia pastoris	9
Ancestral sequence reconstruction and spatial structure analysis guided alteration of longer-chain substrate catalysis for Thermomicrobium roseum lipase	9
A new continuous assay for quantitative assessment of enzymatic degradation of poly(ethylene terephthalate) (PET)	9
Editorial Board	9
Prodigiosin As N-heterocyclic compound: Production optimization, bioactivity evaluation, and in-silico docking against key enzymes related to inflammation, obesity, diabetes, and the insulin signaling	9
Editorial Board	9
Biochemical characterization of the β-glucosidase Glu1B from Coptotermes formosanus produced in Pichia pastoris	9
A study of targeted mutation of l-rhamnose isomerase to improve the conversion efficiency of D-allose	9
Mechanism of tyrosine 69 in the flavin domain modulating electron transfer efficiency in BVU5 azoreductase	9
Identification of carotenoids biosynthesis pathway in Schizochytrium sp. and utilization in astaxanthin biosynthesis	9
Isolation, cloning, and characterization of a novel GH5 cellulase from yak rumen metagenome for enhanced lignocellulose hydrolysis in biofuel production and ruminant feed utilization	9
Utilization of sweet sorghum juice as a carbon source for enhancement of itaconic acid production in engineered Corynebacterium glutamicum	9
A thermophilic phosphatase from Methanothermobacter marburgensis and its application to in vitro biosynthesis	9
Poly(3-hydroxybutyrate) production for food packaging from biomass derived carbohydrates by cupriavidus necator DSM 545	9
Identification of 2’-deoxyguanosine for an α-amylase inhibitor in the extracts of the earthworm Eisenia fetida and characterization of its inhibitory activity against porcine pancreatic α-amylase	9
Immobilization on magnetic PVA/SA@Fe3O4 hydrogel beads enhances the activity and stability of neutral protease	9
Improvement in salt-tolerance of Aspergillus oryzae γ-glutamyl transpeptidase via protein chimerization with Aspergillus sydowii homolog	9
A green pathway for lignin valorization: Enzymatic lignin depolymerization in biocompatible ionic liquids and deep eutectic solvents	9
Synergistic action of thermophilic pectinases for pectin bioconversion into D-galacturonic acid	9