Enzyme and Microbial Technology

Article	Citations
A review on preparation and chemical analysis of postbiotics from lactic acid bacteria	78
A new heterofunctional support for enzyme immobilization: PEI functionalized Fe3O4 MNPs activated with divinyl sulfone. Application in the immobilization of lipase from Thermomyces lanuginosus	75
Structural bioinformatics-based protein engineering of thermo-stable PETase from Ideonella sakaiensis	73
Secretory expression in Bacillus subtilis and biochemical characterization of a highly thermostable polyethylene terephthalate hydrolase from bacterium HR29	68
Plant chitinases and their role in plant defense: A comprehensive review	61
Enzyme immobilized nanomaterials as electrochemical biosensors for detection of biomolecules	61
Microbial lignin peroxidases: Applications, production challenges and future perspectives	52
Micro/Nano fabricated cantilever based biosensor platform: A review and recent progress	52
Porous biochar/chitosan composites for high performance cellulase immobilization by glutaraldehyde	47
Decolourization and biodegradation of methylene blue dye by a ligninolytic enzyme-producing Bacillus thuringiensis: Degradation products and pathway	45
Recent advances in the discovery, characterization, and engineering of poly(ethylene terephthalate) (PET) hydrolases	39
Efficient keratinase expression via promoter engineering strategies for degradation of feather wastes	36
Use of polyethylenimine to produce immobilized lipase multilayers biocatalysts with very high volumetric activity using octyl-agarose beads: Avoiding enzyme release during multilayer production	35
Production and bioprocess optimization of antitumor Epothilone B analogue from Aspergillus fumigatus, endophyte of Catharanthus roseus, with response surface methodology	33
Microbial demethylation of lignin: Evidence of enzymes participating in the removal of methyl/methoxyl groups	31
CRISPR/Cas9 facilitates rapid generation of constitutive forms of transcription factors in Aspergillus niger through specific on-site genomic mutations resulting in increased saccharification of plant	31
High efficiency CRISPR/Cas9 genome editing system with an eliminable episomal sgRNA plasmid in Pichia pastoris	30
Metabolic engineering of Pichia pastoris for production of isopentanol (3-Methyl-1-butanol)	30
Modified silicates and carbon nanotubes for immobilization of lipase from Rhizomucor miehei: Effect of support and immobilization technique on the catalytic performance of the immobilized biocatalysts	29
Bioinspired green synthesis of silver nanoparticles by using a native Bacillus sp. strain AW1-2: Characterization and antifungal activity against Colletotrichum falcatum Went	28
Decyl esters production from soybean-based oils catalyzed by lipase immobilized on differently functionalized rice husk silica and their characterization as potential biolubricants	28
Improving the thermostability of a GH11 xylanase by directed evolution and rational design guided by B-factor analysis	27
Use of biocompatible redox-active polymers based on carbon nanotubes and modified organic matrices for development of a highly sensitive BOD biosensor	26
In situ H2O2 generation methods in the context of enzyme biocatalysis	26
Taguchi design-assisted immobilization of Candida rugosa lipase onto a ternary alginate/nanocellulose/montmorillonite composite: Physicochemical characterization, thermal stability and reusability stu	26

A novel β-glucosidase from a hot-spring metagenome shows elevated thermal stability and tolerance to glucose and ethanol	24
Saturation mutagenesis to improve the degradation of azo dyes by versatile peroxidase and application in form of VP-coated yeast cell walls	23
Adsorption of enzymes with hydrolytic activity on polyethylene terephthalate	23
Whole-cell biocatalyst for cadaverine production using stable, constitutive and high expression of lysine decarboxylase in recombinant Escherichia coli W3110	22
An enzyme-mediated controlled release system for curcumin based on cyclodextrin/cyclodextrin degrading enzyme	22
Biomimetic and biopolymer-based enzyme encapsulation	22
N-acylation of L-amino acids in aqueous media: Evaluation of the catalytic performances of Streptomyces ambofaciens aminoacylases	22
Improving the enzyme property of D-allulose 3-epimerase from a thermophilic organism of Halanaerobium congolense through rational design	21
Current understanding of the cyanobacterial CRISPR-Cas systems and development of the synthetic CRISPR-Cas systems for cyanobacteria	21
A review on enzyme-producing lactobacilli associated with the human digestive process: From metabolism to application	21
Determination of phenol biodegradation pathways in three psychrotolerant yeasts, Candida subhashii A011, Candida oregonensis B021 and Schizoblastosporion starkeyi-henricii L012, isolated from Rucianka	21
Immobilization of Agrobacterium tumefaciens d-psicose 3-epimerase onto titanium dioxide for bioconversion of rare sugar	21
Engineered Bacillus subtilis harbouring gene of d-tagatose 3-epimerase for the bioconversion of d-fructose into d-psicose through fermentation	21
Effect of codon optimization and promoter choice on recombinant endo-polygalacturonase production in Pichia pastoris	21
Role of the lactobacilli in food bio-decontamination: Friends with benefits	20
Production of indigo through the use of a dual-function substrate and a bifunctional fusion enzyme	20
Quercetin in the form of a nano-antioxidant (QTiO2) provides stabilization of quercetin and maximizes its antioxidant capacity in the mouse fibroblast model	20
Cloning, expression and biochemical characterization of lignin-degrading DyP-type peroxidase from Bacillus sp. Strain BL5	19
Improving the activity and stability of Bacillus clausii alkaline protease using directed evolution and molecular dynamics simulation	19
Improving the catalytic activity of thermostable xylanase from Thermotoga maritima via mutagenesis of non-catalytic residues at glycone subsites	19
QQ-PAC core-shell structured quorum quenching beads for potential membrane antifouling properties	19
Recombinant chimeric enzymes for lignocellulosic biomass hydrolysis	18
Enhancement of pipecolic acid production by the expression of multiple lysine cyclodeaminase in the Escherichia coli whole-cell system	18
CRISPR-Cas9-mediated seb1 disruption in Talaromyces pinophilus EMU for its enhanced cellulase production	18
Engineering of l-threonine aldolase for the preparation of 4-(methylsulfonyl)phenylserine, an important intermediate for the synthesis of florfenicol and thiamphenicol	18
Bacterial response to formaldehyde in an MFC toxicity sensor	18
Properties of d-allulose 3-epimerase mined from Novibacillus thermophilus and its application to synthesis of d-allulose	18
Preparation of CLEAs and magnetic CLEAs of a recombinant l-arabinose isomerase for d-tagatose synthesis	18
Nanocomposite membrane and microbial community analysis for improved performance in microbial fuel cell	17
Gamma aminobutyric acid (GABA) production in Escherichia coli with pyridoxal kinase (pdxY) based regeneration system	17
Enhanced biotransformation of the minor ginsenosides in red ginseng extract by Penicillium decumbens β-glucosidase	17
Enhancement of PET biodegradation by anchor peptide-cutinase fusion protein	17
UV-Vis detection of hydrogen peroxide using horseradish peroxidase/copper phosphate hybrid nanoflowers	17
Creation of a robust and R-selective ω-amine transaminase for the asymmetric synthesis of sitagliptin intermediate on a kilogram scale	17
Cloning and expression analysis of fused holin-endolysin from RL bacteriophage; Exhibits broad activity against multi drug resistant pathogens	17
Symbiotic chitin degradation by a novel anaerobic thermophilic bacterium Hydrogenispora sp. UUS1-1 and the bacterium Tepidanaerobacter sp. GT38	16
Synergistic codon optimization and bioreactor cultivation toward enhanced secretion of fungal lignin peroxidase in Pichia pastoris: Enzymatic valorization of technical (industrial) lignins	16
Engineering the heparin-binding pocket to enhance the catalytic efficiency of a thermostable heparinase III from Bacteroides thetaiotaomicron	16
Enzymes in nearly anhydrous deep eutectic solvents: Insight into the biocompatibility and thermal stability	16
Comprehensive investigations of 2-phenylethanol production by high 2-phenylethanol tolerating Meyerozyma sp. strain YLG18	16
Lactobacillus amylovorus derived lipase-mediated silver derivatization over poly(ε-caprolactone) towards antimicrobial coatings	16
Highly sensitive detection of glucose via glucose oxidase immobilization onto conducting polymer-coated composite polyacrylonitrile nanofibers	16
Enzymatic biosensor based on dendritic gold nanostructure and enzyme precipitation coating for glucose sensing and detection	16
Efficient biosynthesis of lacto-N-neotetraose by a novel β-1,4-galactosyltransferase from Aggregatibacter actinomycetemcomitans NUM4039	16
Engineering of L-glutamate oxidase as the whole-cell biocatalyst for the improvement of α-ketoglutarate production	16
High level and enantioselective production of L-phenylglycine from racemic mandelic acid by engineered Escherichia coli using response surface methodology	15
Structural studies of Myceliophthora Thermophila Laccase in the presence of deep eutectic solvents	15
Preparation of phytic acid modified α-Glucosidase/Cu3(PO4)2·3H2O hybrid nanoflower and its application	15
Functional characterisation and product specificity of Endo-β-1,3-glucanase from alkalophilic bacterium, Bacillus lehensis G1	15
pMOX: a new powerful promoter for recombinant protein production in yeast Pichia pastoris	15

Production and characterisation of a novel actinobacterial DyP-type peroxidase and its application in coupling of phenolic monomers	15
Combinatorial engineering for efficient production of protein-glutaminase in Bacillus subtilis	15
Relation between pellet fragmentation kinetics and cellulolytic enzymes production by Aspergillus niger in conventional bioreactor with different impellers	15
Comparative analysis of two recombinant LPMOs from Aspergillus fumigatus and their effects on sugarcane bagasse saccharification	14
Production and in vitro evaluation of prebiotic manno-oligosaccharides prepared with a recombinant Aspergillus niger endo-mannanase, Man26A	14
Efficient production of lactobionic acid using genetically engineered Pseudomonas taetrolens as a whole-cell biocatalyst	14
Expression and purification of asprosin in Pichia pastoris and investigation of its increase glucose uptake activity in skeletal muscle through activation of AMPK	14
Characterization of a biosurfactant producing electroactive Bacillus sp. for enhanced Microbial Fuel Cell dye decolourisation	14
Insights into the effects of acetate on the community structure of Candidatus Accumulibacter in biological phosphorus removal system using DNA stable-isotope probing (DNA-SIP)	14
Characterization of a thermostable phytase from Bacillus licheniformis WHU and further stabilization of the enzyme through disulfide bond engineering	14
Preparation of efficient, stable, and reusable copper-phosphotriesterase hybrid nanoflowers for biodegradation of organophosphorus pesticides	14
Engineering the probiotic bacterium Escherichia coli Nissle 1917 as an efficient cell factory for heparosan biosynthesis	14
Ternary biogenic silica/magnetite/graphene oxide composite for the hyperactivation of Candida rugosa lipase in the esterification production of ethyl valerate	13
Rational engineering of cofactor specificity of glutamate dehydrogenase for poly-γ-glutamic acid synthesis in Bacillus licheniformis	13
Combinatorial engineering for improved menaquinone-4 biosynthesis in Bacillus subtilis	13
Hyaluronic acid-coated chitosan nanoparticles as carrier for the enzyme/prodrug complex based on horseradish peroxidase/indole-3-acetic acid: Characterization and potential therapeutic for bladder can	13
Optimization of ribosomal binding site sequences for gene expression and 4-hydroxyisoleucine biosynthesis in recombinant corynebacterium glutamicum	13
A novel application of simple submersible yeast-based microbial fuel cells as dissolved oxygen sensors in environmental waters	13
Screening and characterization of a GH78 α-l-rhamnosidase from Aspergillus terreus and its application in the bioconversion of icariin to icaritin with recombinant β-glucosidase	13
Antibacterial activity of emulsions containing unsaturated fatty acid ergosterol esters synthesized by lipase-mediated transesterification	13
l-amino acid ligase: A promising alternative for the biosynthesis of l-dipeptides	13
Metabolic engineering of a novel strain of electrogenic bacterium Arcobacter butzleri to create a platform for single analyte detection using a microbial fuel cell	13
Application of magnetic immobilized enzyme of nano dialdehyde starch in deacidification of rice bran oil	12
Biochemical and structural characterization of a novel thermophilic and acidophilic β-mannanase from Aspergillus calidoustus	12
Thermoplastic starch (TPS) bioplastic, the green solution for single-use petroleum plastic food packaging – A review	12
Sequence-based bioprospecting of myo-inositol oxygenase (Miox) reveals new homologues that increase glucaric acid production in Saccharomyces cerevisiae	12
Enhancement of neuroprotection, antioxidant capacity, and water-solubility of crocins by transglucosylation using dextransucrase under high hydrostatic pressure	12
Immobilization on magnetic PVA/SA@Fe3O4 hydrogel beads enhances the activity and stability of neutral protease	12
γ-Glutamyltranspeptidase from Bacillus amyloliquefaciens: transpeptidation activity enhancement and L-theanine production	12
Electrochemical detection of gram-negative bacteria through mastoparan-capped magnetic nanoparticle	12
Non-covalent and covalent immobilization of papain onto Ti3C2 MXene nanosheets	12
cAMP-PKA and HOG1 signaling pathways regulate liamocin production by different ways via the transcriptional activator Msn2 in Aureobasidium melanogenum	12
A fast and easy strategy for lytic polysaccharide monooxygenase-cleavable His6-Tag cloning, expression, and purification	12
The polyhydroxyalkanoate (PHA) synthase 1 of Pseudomonas sp. H9 synthesized a 3-hydroxybutyrate-dominant hybrid of short- and medium-chain-length PHA	12
A facile and one-pot aqueous phase transfer of oleylamine capped Au NP with aminophenylboronic acid used as transfer and targeting ligand	12
Engineering and optimization of phosphate-responsive phytase expression in Pichia pastoris yeast for phytate hydrolysis	11
Microbial biosensor for Salmonella using anti-bacterial antibodies isolated from human serum	11
Production of optically pure (–)-borneol by Pseudomonas monteilii TCU-CK1 and characterization of borneol dehydrogenase involved	11
Rational design of Shewanella sp. l-arabinose isomerase for d-galactose isomerase activity under mesophilic conditions	11
Directed evolution of the transcription factor Gal4 for development of an improved transcriptional regulation system in Saccharomyces cerevisiae	11
Enhancement of carotenoid production and its regulation in edible mushroom Cordyceps militaris by abiotic stresses	11
Identification and catalytic properties of new epoxide hydrolases from the genomic data of soil bacteria	11
High-level heterologous expression of active Chaetomium thermophilum FDH in Pichia pastoris	11
A novel green approach for fabricating visible, light sensitive nano-broccoli-like antimony trisulfide by marine Sb(v)-reducing bacteria: Revealing potential self-purification in coastal zones	11
The effects on antioxidant enzymes of PMMA/hydroxyapatite nanocomposites/composites	11
Comparative characterization of glyoxal oxidase from Phanerochaete chrysosporium expressed at high levels in Pichia pastoris and Trichoderma reesei	11
Lipases of Endophytic Fungi Stemphylium lycopersici and Sordaria sp.: Application in the synthesis of solketal derived Monoacylglycerols	11
Synthesis of improved long-chain isomaltooligosaccharide, using a novel glucosyltransferase derived from Thermoanaerobacter thermocopriae, with maltodextrin	11
A new continuous assay for quantitative assessment of enzymatic degradation of poly(ethylene terephthalate) (PET)	11
A novel chondroitin AC lyase from Pedobacter xixiisoli: Cloning, expression, characterization and the application in the preparation of oligosaccharides	11
Cyanide-free synthesis of aromatic nitriles from aldoximes: Discovery and application of a novel heme-containing aldoxime dehydratase	11
Use of green light to improve the production of lignocellulose-decay enzymes by Pleurotus spp. in liquid cultivation	11
Immobilization of Thermomyces lanuginosus lipase on a new hydrophobic support (Streamline phenyl™): Strategies to improve stability and reusability	10
High-level production of glucose oxidase in Pichia pastoris: Effects of Hac1p overexpression on cell physiology and enzyme expression	10
Equipment-free quantitative determination of urea based on paper-based sensor via urease-mediated chitosan viscosity change	10
Periplasmic expression of Pseudomonas fluorescens peroxidase Dyp1B and site-directed mutant Dyp1B enzymes enhances polymeric lignin degradation activity in Pseudomonas putida KT2440	10
A wheat bran inducible expression system for the efficient production of α-L-arabinofuranosidase in Bacillus subtilis	10
Amylosucrase from Deinococcus geothermalis can be modulated under different reaction conditions to produce novel quercetin 4′-O-α-d-isomaltoside	10
Preference for particular lanthanide species and thermal stability of XoxFs in Methylorubrum extorquens strain AM1	10
A novel growth and isolation medium for exoelectrogenic bacteria	10
Characterizations of the endolysin Lys84 and its domains from phage qdsa002 with high activities against Staphylococcus aureus and its biofilms	10
Efficient oxidation of 5-hydroxymethylfurfural to 2,5-furandicarboxylic acid by a two-enzyme system: Combination of a bacterial laccase with catalase	10
Improving (2S)-naringenin production by exploring native precursor pathways and screening higher-active chalcone synthases from plants rich in flavonoids	10
Ancestral sequence reconstruction and spatial structure analysis guided alteration of longer-chain substrate catalysis for Thermomicrobium roseum lipase	10
Identification of carotenoids biosynthesis pathway in Schizochytrium sp. and utilization in astaxanthin biosynthesis	10
Kinetic and thermodynamic study of laccase cross-linked onto glyoxyl Immobead 150P carrier: Characterization and application for beechwood biografting	10
Pichia pastoris Protein Disulfide Isomerase (PDI1) promoter for heterologous protein production and its sequence characterization	10
High D-arabitol production with osmotic pressure control fed-batch fermentation by Yarrowia lipolytica and proteomic analysis under nitrogen source perturbation	10
Expression of a novel α-glucosidase from Aspergillus neoniger in Pichia pastoris and its efficient recovery for synthesis of isomaltooligosaccharides	10
Cytochrome P450 5208A3 is a promiscuous xenobiotic biotransforming enzyme in Cunninghamella elegans	10
Enzymatic production of 3’-sialyllactose in milk	10
De novo biosynthesis of linalool from glucose in engineered Escherichia coli	10
Combinational expression of D-sorbitol dehydrogenase and pyrroloquinoline quinone increases 6-(N-hydroxyethyl)-amino-6-deoxy-α-L-sorbofuranose production by Gluconobacter oxydans through cofactor mani	10
Synthesis, characterization and immobilization of bilirubin oxidase nanoparticles (BOxNPs) with enhanced activity: Application for serum bilirubin determination in jaundice patients	9
Identification of a lactose-oxidizing enzyme in Escherichia coli and improvement of lactobionic acid production by recombinant expression of a quinoprotein glucose dehydrogenase from Pseudomonas taetr	9
Engineered P450 BM3 and cpADH5 coupled cascade reaction for β-oxo fatty acid methyl ester production in whole cells	9
Characteristics of a fructose 6-phosphate 4-epimerase from Caldilinea aerophila DSM 14535 and its application for biosynthesis of tagatose	9
Methods of detection of β-galactosidase enzyme in living cells	9
An artificial multi-enzyme cascade biocatalysis for biomanufacturing of nicotinamide mononucleotide from starch and nicotinamide in one-pot	9
Enhanced removal of hydrocarbons BTX by light-driven Aspergillus niger ZnS nanobiohybrids	9

A lipase/poly (ionic liquid)-styrene microspheres/PVA composite hydrogel for esterification application	9
Cholesterol oxidase immobilized inulin based nanocomposite as the sensing material for cholesterol in biological and food samples	9
Glucose oxidase-mediated sodium alginate gelation: Equipment-Free detection of glucose in fruit samples	9
Construction of lactic acid overproducing Clostridium thermocellum through enhancement of lactate dehydrogenase expression	9
One-pot production of maltoheptaose (DP7) from starch by sequential addition of cyclodextrin glucotransferase and cyclomaltodextrinase	9
Direct and selective enzymatic synthesis of trehalose unsaturated fatty acid diesters and evaluation of foaming and emulsifying properties	9
Construction of novel curdlan-based and Ca2+-chelated magnetic microspheres (CCMM) for efficient protein purification and oriented immobilization	9
Geotrichum candidum aldehyde dehydrogenase-inorganic nanocrystal with enhanced activity	9
Microbial enrichment and meta-omics analysis identify CAZymes from mangrove sediments with unique properties	9
Enzymatic epoxidation of cyclohexene by peroxidase immobilization on a textile and an adapted reactor design	9
High stabilization and hyperactivation of a Recombinant β-Xylosidase through Immobilization Strategies	9
Preparation of a Bombyx mori acetylcholinesterase enzyme reagent through chaperone protein disulfide isomerase co-expression strategy in Pichia pastoris for detection of pesticides	9
Whole-cell biosynthesis of d-tagatose from maltodextrin by engineered Escherichia coli with multi-enzyme co-expression system	9
Design of a PL18 alginate lyase with flexible loops and broader entrance to enhance the activity and thermostability	9
Enhanced catalytic efficiency and thermostability of glucose isomerase from Thermoanaerobacter ethanolicus via site-directed mutagenesis	9
Functional expression and characterization of two laccases from the brown rot Fomitopsis pinicola	9
Peptide ligases: A Novel and potential enzyme toolbox for catalytic cross-linking of protein/peptide-based biomaterial scaffolds for tissue engineering	9
Improved thermostability, acid tolerance as well as catalytic efficiency of Streptomyces rameus L2001 GH11 xylanase by N-terminal replacement	9
Characterization of a recombinant D-mannose-producing D-lyxose isomerase from Caldanaerobius polysaccharolyticus	9
One pot purification and co-immobilization of His-tagged old yellow enzyme and glucose dehydrogenase for asymmetric hydrogenation	9