OOIR: Observatory of International Research

Papers

(The TQCC of Enzyme and Microbial Technology is 9. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2022-06-01 to 2026-06-01.)

Article	Citations
Microbial engineering for shikimate biosynthesis	207
Analysis of enzyme kinetics of fungal methionine synthases in an optimized colorimetric microscale assay for measuring cobalamin-independent methionine synthase activity	73
More efficient barley malting under catalyst: Thermostability improvement of a β-1,3-1,4-glucanase through surface charge engineering with higher activity	44
Osmoregulation by choline-based deep eutectic solvent induces electroactivity in Bacillus subtilis biofilms	42
Metataxonomic characterization of an autochthonous and allochthonous microbial consortium involved in a two-stage anaerobic batch reactor applied to hydrogen and methane production from sugarcane baga	40
PhcK mediates transport of a β-5-type lignin-derived dimer in Sphingobium lignivorans SYK-6	38
Immobilization of α-glucosidase on polystyrene plates: A practical application to α-amylase detection	38
Structure and functional role of the SLHs-CBM54 tandem in bacterial multimodular glycoside hydrolases	37
Characterization of a novel short-chain dehydrogenase from Sugiyamaella lignohabitans and its application in the degradation of patulin	35
Carbohydrate-active enzyme-catalyzed stereoselective glycosylation of complex natural product glycosides	35
Enhancing the thermal stability and activity of zearalenone lactone hydrolase to promote zearalenone degradation via semi-rational design	35
Study on bio-imprinted Aspergillus niger lipase cross-linked aggregates and catalytic synthesis of Vitamin E succinate	33
Latent potentials of the white-rot basidiomycete Phanerochaete chrysosporium responsible for sesquiterpene metabolism: CYP5158A1 and CYP5144C8 decorate (E)-α-bisabolene	31
Biochar production from microalgae: a new sustainable approach to wastewater treatment based on a circular economy	30
Molecular cloning and characterization of a novel bifunctional cellobiohydrolase/β-xylosidase from a metagenomic library of mangrove soil	30
Insights on kraft lignin degradation in an anaerobic environment	29
Alcalase immobilization in iota-carrageenan-matrix hydrogel beads derived from the macroalga Solieria filiformis	29
Microbial production of adipic acid from 6-hydroxyhexanoic acid for biocatalytic upcycling of polycaprolactone	28
Tailoring of levansucrase product size by a comparative molecular dynamics approach	27
Highly sensitive detection of glucose via glucose oxidase immobilization onto conducting polymer-coated composite polyacrylonitrile nanofibers	27
Revolutionizing recombinant protein production in prokaryotic platforms – Methodologies and advances	27
Editorial Board	26
Improvement of lipid production from glucose/xylose mixed-sugar by the oleaginous yeast Lipomyces starkeyi through ultra-violet mutagenesis	26
Modulating dioxygenase and hydroperoxide isomerase activities in Burkholderia thailandensis lipoxygenase	25
Synergy of GH67 and GH115 α-1,2-glucuronidases with Penicillium subrubescens endoxylanases to stimulate xylooligosaccharide production	24

Cloning, purification and characterization of a novel thermostable recombinant tannase from Galactobacillus timonensis	23
Exploring Helicobacter pylori Histidyl tRNA synthetase as a drug target using biophysical and computational approaches	23
Divergent substrate specificities and regioselectivities of three lipase isoforms from Cordyceps militaris: Combinatorial advantages for entomopathogenicity and prospects as biocatalysts	23
Robust cross-linked cyclodextrin glucanotransferase from Bacillus lehensis G1 aggregates using an improved cross-linker and a new co-aggregant for the production of cyclodextrins	22
Structural and functional insights of the catalytic GH5 and Calx-β domains from the metagenome-derived endoglucanase CelE2	22
Identification of a bacteria P450 enzyme from B. megaterium H-1 with vitamin D3 C-25 hydroxylation capabilities	22
Development and expansion of the CRISPR/Cas9 toolboxes for powerful genome engineering in yeast	22
Isolation of a newly Trichoderma asperellum LYS1 with abundant cellulase-hemicellulase enzyme cocktail for lignocellulosic biomass degradation	21
Structures and properties of α-amylase and glucoamylase immobilized by ZIF-8 via one-pot preparation	21
Improving the cellobiose hydrolysis activity of glucose-stimulating β-glucosidase Bgl2A	21
The potential of R. toruloides mevalonate pathway genes in increasing isoprenoid yields in S. cerevisiae: Evaluation of GGPPS and HMG-CoA reductase	21
Editorial Board	20
Editorial Board	20
Efficient production of γ-aminobutyric acid using engineered Escherichia coli whole-cell catalyst	19
Rational design improves both thermostability and activity of a new D-tagatose 3-epimerase from Kroppenstedtia eburnean to produce D-allulose	19
Semi-rational engineering of ω-transaminase for enhanced enzymatic activity to 2-ketobutyrate	19
Influence of a rumen ciliate-derived xylanase on the gut microbiota composition: A potential enzyme for prebiotic applications	18
Release of bifidogenic N-glycans from native bovine colostrum proteins by an endo-β-N-acetylglucosaminidase	18
Simultaneous enhancement of thermostability and catalytic activity of κ-carrageenase from Pseudoalteromonas tetraodonis by rational design	18
Mutational analysis of predicted active site residues of an exoglucanase secreted by Xanthomonas oryzae pv. oryzae to determine their role in catalysis and in virulence on rice	18
Production of recombinant glycosidases fused with Usp45 and SpaX to avoid the purification and immobilization stages	18
Enzyme immobilization technology as a tool to innovate in the production of biofuels: A special review of the Cross-Linked Enzyme Aggregates (CLEAs) strategy	17
Application of reductive amination by heterologously expressed Thermomicrobium roseum L-alanine dehydrogenase to synthesize L-alanine derivatives	17
Design of a synthetic enzyme cascade for the in vitro fixation of formaldehyde to acetoin	17
Mutational and structural analysis of ribose 5-phosphate isomerase B from Acetivibrio thermocellus: relationship between transformation efficiency and substrate binding pocket conformation	17
Corrigendum to “Kinetic and thermodynamic study of laccase cross-linked onto glyoxyl Immobead 150P carrier: Characterization and application for beechwood biografting” [Enzym. Microb. Technol. 150 (20	17
Periplasmic expression of Pseudomonas fluorescens peroxidase Dyp1B and site-directed mutant Dyp1B enzymes enhances polymeric lignin degradation activity in Pseudomonas putida KT2440	17
Editorial Board	17
Non-native genetic configuration of Gluconobacter oxydans dehydrogenases drives 2-keto-L-gulonic acid production in recombinant Escherichia coli	17
Rational design of short-chain dehydrogenase DHDR for efficient synthesis of (S)-equol	16
Investigation of biocatalytic production of lactosucrose and fructooligosaccharides using levansucrases and dairy by-products as starting materials	16
Comparing similar versions of a connecting helix on the structure of Chondroitinase ABC I	16
Characterization and application of active human α2,6-sialyltransferases ST6GalNAc V and ST6GalNAc VI recombined in Escherichia coli	16
Comparative evaluation of l-theanine synthetases coupled with PPK2 based ATP regeneration under buffer-free and Mn2 + optimized conditions	16
Operation parameters and temperature affected sludge microbial metabolisms: An integrated perspective considering extracellular polymeric substances, soluble microbial products, biomass quantities, an	16
Enhancement of rice husks saccharification through hydrolase preparation assisted by lytic polysaccharide monooxygenase	16
Nanotechnology based technological development in biofuel production: Current status and future prospects	16
Integrated bio-nano remediation of produced water using Alcanivorax borkumensis and GQDs-modified ZnO/La₂O₃ nanocomposites	16
Improved thermostability of type I pullulanase from Bacillus thermoliquefaciens by error-prone PCR	15
Microbial community analysis of the biofilms of both working and counter electrodes in single-chamber microbial electrolysis cells	15
Enzymatic production of chitooligosaccharides with high degree of polymerisations and their potential application to soy sauce preservation	15
Metabolism of phenolic compounds catalyzed by Tomato CYP736A61	15
Protein engineering of an alkaline protease from Bacillus licheniformis (BLAP) for efficient and specific chiral resolution of the racemic ethyl tetrahydrofuroate	15
A UPLC-based activity assay method for aminoglycoside phosphotransferase 4-Ia (APH4): A Selectable marker of plant transformation	15
Enzymatic determination of hypoxanthine in fish samples as a freshness indicator using the CUPRAC colorimetric sensor	15
Metagenomic insight on consortium degradation of soil weathered petroleum and its supplement based on gene abundance change	15
Coriolus versicolor laccase-based inorganic protein hybrid synthesis for application in biomass saccharification to enhance biological production of hydrogen and ethanol	14
RETRACTED: Identification and recombinant expression of a novel defluorinase from Rhodococcus jostii RHA1, for defluorination and biotransformation of the PFAS compound 6:2 fluorotelomer carboxylic ac	14
Targeted gene manipulation of Leloir pathway genes for the constitutive expression of β-galactosidase and its transgalactosylation product galacto-oligosaccharides from Kluyveromyces lactis GG799 and	14
Dual-acting single-engineered hybrid-architectured promoters enhance and convert expressions into multi-carbon source-regulated systems in Komagataella phaffii	14

NADPH regeneration for efficient biosynthesis of indigo by flavin-containing monooxygenase and formate dehydrogenase	14
Construction and characterization of a Myceliophthora thermophila lytic polysaccharide monooxygenase mutant S174C/A93C with improved thermostability	13
Using large-scale multi-module NRPS to heterologously prepare highly efficient lipopeptide biosurfactants in recombinant Escherichia coli	13
Penicillin binding proteins-based immunoassay for the selective and quantitative determination of beta-lactam antibiotics	13
Action pattern of Sulfolobus O-α-glycoligase for synthesis of highly water soluble resveratrol 3,4′-α-diglucoside	13
Probiotic and functional characterization of newly isolated Lactiplantibacillus plantarum strains from human breast milk and proliferative inhibition potential of metabolites	13
Editorial Board	13
Improved thermal stability of manganese superoxide dismutase from Staphylococcus equorum through formation of artificial oligomer	13
Engineering of ω-transaminase at binding pocket and access tunnel for preparation of (R)-1-phenoxypropan-2-amine	13
Mutational study-based identification of high activity O-methyltransferase for the regioselective methylation of epigallocatechin gallate	13
Immobilization of Thermomyces lanuginosus lipase on a new hydrophobic support (Streamline phenyl™): Strategies to improve stability and reusability	12
Unveiling steps of the TDP degradation pathway in Variovorax paradoxus TBEA6	12
Anti-biofilm efficiency and substrate specificity of recombinantly produced human paraoxonases	12
Screening β-glucosidase and α-rhamnosidase for biotransformation of naringin to naringenin by the one-pot enzymatic cascade	12
Agmatine production by Escherichia coli cells expressing SpeA on the extracellular surface	12
Continuous production of gamma aminobutyric acid by engineered and immobilized Escherichia coli whole-cells in a small-scale reactor system	12
Enzymatic hydrolysis of milk thistle protein: Influence of protease types on structure and biological activity	12
Identification and characterization of endo-xylanases from families GH10 and GH11 sourced from marine thermal environments	12
The increased efficiency of porphyran hydrolysis by constructing a multifunctional enzyme complex from marine microorganisms	12
Biocatalytic valorization of lignin subunit: Screening a carboxylic acid reductase with high substrate preference to syringyl functional group	12
Development of a chloroplast expression system for Dunaliella salina	12
A Deep Learning Multi-Attention Bi-GRU Framework for kcat Prediction with Segmentation-Based Insights	12
Editorial Board	11
Whole-cell bioconversion for producing thymoquinone by engineered Saccharomyces cerevisiae	11
Biochemical characterisation of glycosylated and deglycosylated forms of phytase from Cronobacter turicensis expressed in Pichia pastoris	11
Carbohydrate-binding modules of ChiB and ChiC promote the chitinolytic system of Serratia marcescens BWL1001	11
Editorial Board	11
Rhamnosyltransferases: Biochemical activities, potential biotechnology for production of natural products and their applications	11
Disruption of vacuolar protein sorting receptor gene Poxvps10 improves cellulolytic enzyme production by Penicillium oxalicum	11
Efficient snailase-based production of mogrol from Luo Han Guo extract in an aqueous-organic system	11
Efficient production of isoquercitin, icariin and icariside II by a novel thermostable α-l-rhamnosidase PodoRha from Paenibacillus odorifer with high α-1, 6-/α-1, 2- glycoside specificity	11
Editorial Board	11
Mutant β-fructofuranosidase synthesizing blastose [β-d-Fruf-(2→6)-d-Glcp]	11
Enzymatic lacto-N-biose elongation of human milk oligosaccharides with the GH136 lacto-N-biosidase LnbX engineered for improved transglycosylation	11
Biotransformation of ferulic acid into vanillyl alcohol and vanillic acid employing thermophilic bacterium Caldimonas thermodepolymerans	11
Non-covalent binding tags for batch and flow biocatalysis	10
Application of natively expressed chitinase as a sustainable fungal bioshield	10
Co-expression of an isopropanol synthetic operon and eGFP to monitor the robustness of Cupriavidus necator during isopropanol production	10
Utilization of sweet sorghum juice as a carbon source for enhancement of itaconic acid production in engineered Corynebacterium glutamicum	10
The effect of the cspA gene on growth development and butenyl-spinosyn biosynthesis in Saccharopolyspora pogona	10
Biochemical and structural characterization of a family-9 glycoside hydrolase bioprospected from the termite Syntermes wheeleri gut bacteria metagenome	10
CO2 capture and lipid production performance of microalgae in the S-shaped photobioreactor under different culture modes	10
A new continuous assay for quantitative assessment of enzymatic degradation of poly(ethylene terephthalate) (PET)	10
Isolation, cloning, and characterization of a novel GH5 cellulase from yak rumen metagenome for enhanced lignocellulose hydrolysis in biofuel production and ruminant feed utilization	10
Metabolic engineering of Bacillus subtilis for enhanced p-Coumaric acid production and antimicrobial applications	10
Synergistic action of thermophilic pectinases for pectin bioconversion into D-galacturonic acid	10
Semi-rational engineering of 3-Ketosteroid-Δ1-dehydrogenase boosts catalytic efficiency and robustness for steroid bioconversion	10
Using directed evolution to improve hydrogen production in chimeric hydrogenases from algal species	10
Ascorbate oxidase enabling glucometer readout for portable detection of hydrogen peroxide	9
Cell surface display of CAL-B in Escherichia coli using the split GFP system	9
Fungal tyrosinase immobilized on chitosan, calcium alginate, and silica gel for phenol elimination and dye decolorization	9
Prodigiosin As N-heterocyclic compound: Production optimization, bioactivity evaluation, and in-silico docking against key enzymes related to inflammation, obesity, diabetes, and the insulin signaling	9
Nicotinic acid production from 3-methylpyridine by E. coli whole-cell biocatalyst	9
A green pathway for lignin valorization: Enzymatic lignin depolymerization in biocompatible ionic liquids and deep eutectic solvents	9
Sensitive and specific fluorometric assay for assessment of glyoxalase II enzymatic activity in microbial samples and biological tissue	9
A novel and thermostable cyclomaltodextrin glucanotransferase from Anoxybacillus rupiensis PSO2 isolated from Yumthang hot spring: cloning and functional characterisation	9
Biochemical characterization of the β-glucosidase Glu1B from Coptotermes formosanus produced in Pichia pastoris	9
Unraveling the role of E. coli and calf intestinal alkaline phosphatase in calcium phosphate synthesis	9
Production of an O-desmethylated product, a major human metabolite, of rabeprazole sulfide by bacterial P450 enzymes	9
Simultaneous production of linear α-olefins and 2,5-furandicarboxylic acid by combining two recombinant enzymes OleT-ELP and HMFO-ELP	9
A study of targeted mutation of l-rhamnose isomerase to improve the conversion efficiency of D-allose	9
Mechanism of tyrosine 69 in the flavin domain modulating electron transfer efficiency in BVU5 azoreductase	9
A thermophilic phosphatase from Methanothermobacter marburgensis and its application to in vitro biosynthesis	9
The potential and innovative applications of CRISPR gene editing technology in enzyme gene development	9
Improvement in salt-tolerance of Aspergillus oryzae γ-glutamyl transpeptidase via protein chimerization with Aspergillus sydowii homolog	9
Poly(3-hydroxybutyrate) production for food packaging from biomass derived carbohydrates by cupriavidus necator DSM 545	9
A GH19 lysozyme and peptidase from Myoviridae cyanophages lacking the typical holin-endolysin system exhibit lytic activity	9
Decyl esters production from soybean-based oils catalyzed by lipase immobilized on differently functionalized rice husk silica and their characterization as potential biolubricants	9
Editorial Board	9
Cell-free production of the bifunctional glycoside hydrolase GH78 from Xylaria polymorpha	9
Immobilization on magnetic PVA/SA@Fe3O4 hydrogel beads enhances the activity and stability of neutral protease	9
Identification of 2’-deoxyguanosine for an α-amylase inhibitor in the extracts of the earthworm Eisenia fetida and characterization of its inhibitory activity against porcine pancreatic α-amylase	9
Editorial Board	9
Harnessing seaweed-associated marine Streptomyces sp. OSs-1 for nanotechnology-driven cost-effective enzyme production using agricultural waste	9
Heterologous expression, purification, and characterization of a recombinant Cordyceps militaris lipase from Candida rugosa-like family in Pichia pastoris	9
Production and characterization of novel thermostable CotA-laccase from Bacillus altitudinis SL7 and its application for lignin degradation	9
A lateral flow biosensor based on duplex-specific nuclease and novel isothermal amplification for detection of influenza virus	9