OOIR: Observatory of International Research

Papers

(The TQCC of Biotechnology Letters is 5. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2022-05-01 to 2026-05-01.)

Article	Citations
Influence of affinity tags and tobacco PR1a signal peptide on detection, purification and bioactivity analyses of the small oomycete apoplastic effectors	53
Listeria monocytogenes is a solvent tolerant organism secreting a solvent stable lipase: potential biotechnological applications	52
Recombinant expression, downstream optimization, and therapeutic evaluation of recombinant human interleukin-37 for cancer therapy	37
Cell density detection based on a microfluidic chip with two electrode pairs	30
Affinity chromatography studies for Mespilus germanica L. polyphenol oxidase	27
Activation of galactose utilization by the addition of glucose for the fermentation of agar hydrolysate using Lactobacillus brevis ATCC 14869	25
The pedal-like loop of (R)-selective transaminases plays a critical role to the functionality of the enzyme	22
Unraveling the molecular mechanisms of selenite reduction: transcriptomic analysis of Bacillus reveals the key role of sulfur assimilation	19
Silica nanoparticles enhance interfacial self-adherence of a multi-layered extracellular matrix scaffold for vascular tissue regeneration	17
Antisense oligonucleotides: targeting oncogenic long non-coding RNAs for cancer therapy	17
Directed evolution of cholesterol oxidase with improved thermostability using error-prone PCR	16
Synthesis of β-ionone from xylose and lignocellulosic hydrolysate in genetically engineered oleaginous yeast Yarrowia lipolytica	16
Effects of three light–dark cycles on circadian rhythm-related genes and photosynthetic parameters in carrot	16
Design and application of expression constructs for FMDV serotype O structural proteins	15
High throughput screening and metabolic engineering of Saccharopolyspora spinosa for spinosad production	15
Dual functions of nonionic surfactant Tween-20 in enhancing productivity and elution efficiency of alkylresorcinols from Azotobacter vinelandii	14
Efficient immobilization behavior and mechanism investigation of Pb(II) by Aspergillus tubingensis	14
Recombinant erythropoietin expression elevates by ubiquitous chromatin opening element (UCOE) in CHO DG44 cells	14
Paenibacillus barengoltzii: isolation, growth, and characterization of a high dextrinizer α-amylase	14
Application of exogenous melatonin in vitro and in planta: a review of its effects and mechanisms of action	13
Synergistic anticancer effects of interleukin-21 combined with therapeutic peptides in multiple cancer cells	13
Screening of a xylanase-producing Trichoderma strain and optimization of its enzyme production conditions	13
Squalene hopene cyclases and oxido squalene cyclases: potential targets for regulating cyclisation reactions	13
Application of lactose based autoinduction for heterologous production of an active [NiFe] hydrogenase in E. coli	13
Biocontrol of strawberry Botrytis gray mold and prolong the fruit shelf-life by fumigant Trichoderma spp.	13

Enhancing leucine aminopeptidase activity in Aspergillus oryzae LL1 through multi-round UV irradiation mutagenesis with potential application in debittering	13
Factors affecting rAAV titers during triple-plasmid transient transfection in HEK-293 cells	13
Characterization and substrate specificity study of the novel (R)-amine transaminase MagAT	12
Molecular cloning and modelling of a thermostable α-amylase from a thermophilic Geobacillus sp. DS3	12
Sustainable biodegradation of phenol using peroxidases extracted from local agricultural wastes	12
Identification of laccase genes in Trichoderma asperellum Ts93	12
Retraction Note: Autophagy regulated by lncRNA HOTAIR contributes to the cisplatin-induced resistance in endometrial cancer cells	11
Metabolic response of Bacillus spp. to heavy metal stress: pathway alterations and metabolite profiles	11
A rapid analytical method using loop-mediated isothermal amplification (LAMP): visual detection of cry1Ab gene in genetically modified maize seeds and food products	11
Clinical and mechanistic insights into biomedical application of Se-enriched probiotics and biogenic selenium nanoparticles	11
Enhanced gibberellic acid production in Fusarium fujikuroi through ARTP-EMS mutagenesis and fermentation optimization	11
Gene expression profiling to elucidate the promotive effects of the volatile organic compound 3-octanone on the mycelial growth of Ganoderma lucidum	10
Therapeutic potential of a novel IP-10-(anti-HER2 scFv) fusion protein for the treatment of HER2-positive breast cancer	10
Enhancement hispolon production from Phellinus linteus via epigenetic-modified culture to inhibit human breast cancer cells	10
Evaluation of cultivation performance of S. cerevisiae strains expressing GLP-1-GIP peptide precursors	10
Optimizing hyaluronic acid production by Streptococcus zooepidemicus using taguchi method: effects of temperature and pH	10
BMSCs improve TNBS-induced colitis in rats by inducing Treg differentiation by expressing PD-L1	10
Succession from acetoclastic to hydrogenotrophic microbial community during sewage sludge anaerobic digestion for bioenergy production	10
Enhancing lipase activity in Aspergillus niger through CRISPR/Cas9-mediated protease gene knockout and fermentation optimization	10
Isolation, microscopic and magnetotactic characterization of Magnetospirillum moscoviense MS-24 from Banjosa Lake, Pakistan	9
Correction: Biodegradation of ketoprofen using a microalgal–bacterial consortium	9
Eco-friendly azo dye removal via a robust laccase from Acinetobacter baumannii KMSP005: a thermo-alkaliphilic biocatalytic approach	9
Retraction Note: TP53INP1 3′-UTR functions as a ceRNA in repressing the metastasis of glioma cells by regulating miRNA activity	9
Preparation, characterization, and enzymatic properties of laccase immobilized on MnO2 nanoparticles via adsorption method	9
Research on the application of heterotrophic nitrification-aerobic denitrification bacteria in membrane bioreactor (MBR)	9
Gallic acid as biofilm inhibitor can improve transformation efficiency of Ruminiclostridium papyrosolvens	9
Substrate type and CO2 addition significantly influence succinic acid production of Basfia succiniciproducens	9
Optimization of astaxanthin production by Rhodotorula toruloides CB6-10/1 using response surface methodology and its genome analysis	8
Improving the specific activity and stability of alkaline pectinase PEL3 through SpyTag/SpyCatcher cyclization	8
Bone marrow mesenchymal stem cell-derived exosomes loaded with miR-26a through the novel immunomodulatory peptide DP7-C can promote osteogenesis	8
Magnetic particle-based chemiluminescence immunoassay for serum human heart-type fatty acid binding protein measurement	8
Measurement of HbA1c with a new phase function using RBC phase image from a low-cost digital holographic microscopy	8
Application of low-intensity ultrasound to enhance simultaneous nitrification/iron-based autotrophic denitrification	8
Droplet based microfluidic and miniaturization at high-throughput level to screening heterologous secondary metabolites in microbes: A critical review	8
Enhancing production of nicotinamide mononucleotide by metabolic engineering and flow cytometry screening of Escherichia coli	8
Construction of a bacteriophage-derived vector with potential applications in targeted drug delivery and cell imaging	8
The modification of heme special importer to improve the production of active hemoglobins in Escherichia coli	8
Ultrasonic high efficiency preparation of arctigenin from the fruits of Arctium lappa L using Aspergillus niger with deep eutectic solvent assisted	8
Characterization of a novel Escherichia coli recombineering selection/counterselection cassette	8
Micro-electrochemical DO sensor with ultra-micropore matrix fabricated with femtosecond laser processing successfully applied in on-line DO monitoring for yeast culture	8
Toxicants improve glycerol production in the fermentation of undetoxified hydrolysate by Candida glycerinogenes	8
Novel TdsD nitroreductase: characterization of kinetics and substrate specificity	8
Application of CRISPR-Cas9 in microbial cell factories	8
Combining biotechnology with sustainability: feasibility of bioenergy sorghum in generating high-value bioproducts	8
Role of microbial pesticides in pest control for lepidopteran: research status and prospect	8
An artificial multienzyme cascade for the whole-cell synthesis of rare ketoses from glycerol	8
Cellulase and chitinase activities and antagonism against Fusarium oxysporum f.sp. cubense race 1 of six Trichoderma strains isolated from Mexican maize cropping	7
Mutational analysis of pig tissue factor pathway inhibitor α to increase anti-coagulation activity in pig-to-human xenotransplantation	7
Trichoderma longibrachiatum and thermothelomyces thermophilus co-culture: improvement the saccharification profile of different sugarcane bagasse varieties	7
Differentiation, maturation, and collection of THP-1-derived dendritic cells based on a PEG hydrogel culture platform	7

A novel P450 enzyme assay utilizing an NADP+-based biosensor	7
The variation of microorganisms and organics during methane production from lignite under an electric field	7
Advancing the frontier of plant-based therapeutics: critical innovations in molecular farming and bioprocess Integration	7
Economic optimization of expression of soluble human epidermal growth factor in Escherichia coli	7
Dynamic cultivation of human mesenchymal stem/stromal cells for the production of extracellular vesicles in a 3D bioreactor system	7
Aptamer-functionalized MOFs and AI-driven strategies for early cancer diagnosis and therapeutics	7
Development of a synthetic transcription factor-based S-adenosylmethionine biosensor in Saccharomyces cerevisiae	7
Genome-scale metabolic model-based engineering of Escherichia coli enhances recombinant single-chain antibody fragment production	7
Identification, molecular and biochemical characterization of a novel thermoactive and thermostable glucoamylase from Thermoanaerobacter ethanolicus	7
Investigation of osteogenesis and angiogenesis in perfusion bioreactors using improved multi-layer PCL-nHA-nZnO electrospun scaffolds	7
Carrier immobilization and auto-exposition favoring reuse of silyletherase SilE-R from Brassica sp. with high activity and enantiospecificity	6
Antimicrobial activity of thermophilin 110 against the opportunistic pathogen Cutibacterium acnes	6
Competitive inhibition of a non-natural cofactor dependent formaldehyde dehydrogenase by imidazole	6
Biotransformation of C20- and C22-polyunsaturated fatty acids to 11S- and 13S-hydroxy fatty acids by Escherichia coli expressing 11S-lipoxygenase from Enhygromyxa salina	6
Improving the hydrophilic microenvironment surrounding the catalytic site of fructosyltransferase enhances its catalytic ability	6
Expansion of YALIcloneHR toolkit for Yarrowia lipolytica combined with Golden Gate and CRISPR technology	6
Cost-effective production of squalene using Yarrowia lipolytica via metabolic engineering and fermentation engineering	6
Homo- and heterofermentative lactobacilli are distinctly affected by furanic compounds	6
Enhancing HAP3 mediated xylose metabolism and acetic acid metabolism promotes lipid production from lignocellulosic hydrolysate fermented by Candida glycerinogenes	6
A cell-free fluorescence biosensor based on allosteric transcription factor NalC for detection of pentachlorophenol	6
Optimization of ultrasound-assisted production of ergosterol from Penicillium brevicompactum by Taguchi statistical method	6
Optimizing microbial electrolysis cell performance: strategies to mitigate electron mediator degradation on membranes	6
Correction: Polyhydroxyalkanoate production by Paramecium caudatum isolated from industrial wastewater: a micro eukaryotic host for bioplastic accumulation	6
GH2 family β-galactosidases evolution using degenerate oligonucleotide gene shuffling	6
Isolation and characterisation of exosomes from Chinese hamster ovary (CHO) cells	6
Pilot-scale production of Bacillus subtilis MSCL 897 spore biomass and antifungal secondary metabolites in a low-cost medium	6
A gold nanoparticles-based lateral flow assay utilizing baculovirus expressed recombinant nucleocapsid and receptor binding domain proteins for serodetection of IgG and IgM against SARS-CoV-2	6
Molecular cloning and characterization of an alpha-amylase inhibitor (TkAAI) gene from Trichosanthes kirilowii Maxim.	6
Preparation of white rot fungal inoculum and its application to bioremediation of chlorimuron-ethyl-contaminated soil	6
Establishment of a visual gene knockout system based on CRISPR/Cas9 for the rare actinomycete Nonomuraea gerenzanensis	6
A flexible, cost- and time-saving method for in-house laboratory production of DNA markers	6
Effects of the deletion and substitution of thioesterase on bacillomycin D synthesis	6
Genetic engineering of Beauveria bassiana for enhancing siderophore production and its virulence against the insect hosts	6
Medical biotechnology and artificial intelligence powered companion diagnostics: indispensable pillars driving next generation precision health care	5
A single-vector CRISPR/Cas9 system for genome editing and heterologous enzyme secretion in Saccharomyces cerevisiae: a case study on pectate lyase for coffee mucilage removal	5
Efficient and high-density immobilization of animal cells by a microfiber with both swelling and cell adhesion properties and its application to exosome production	5
Roles of PKAc1 and CRE1 in cellulose degradation, conidiation, and yellow pigment synthesis in Trichoderma reesei QM6a	5
Recombinant protein CP01850 adjuvanted with Iridea cordata or Sacorpletis skottibergii lipid extracts protected mice against infection by Corynebacterium pseudotuberculosis	5
swnk plays an important role in the biosynthesis of swainsonine in Metarhizium anisopliae	5
WAVE-based intensified perfusion cell culture for fast process development	5
Improvement of transglutaminase production by controlling the morphology of Streptomyces mobaraensis HVCP-Sm1 with microparticle-enhanced cultivation	5
Anti-inflammatory potential of baicalein combined with silk fibroin protein in a zebrafish model (Danio rerio)	5
Enhanced soluble expression of active recombinant human interleukin-29 using champion pET SUMO system	5
Citrus essential oil: would it be feasible as antimicrobial in the bioethanol industry?	5
Development of an animal-derived component-free medium for Spodoptera frugiperda (Sf9) cells using response surface methodology	5
CRISPR/Cas9-assisted ssDNA recombineering for site-directed mutagenesis and saturation mutagenesis of plasmid-encoded genes	5
Redifferentiation of genetically modified dedifferentiated chondrocytes in a microcavitary hydrogel	5
Bioinformatic evaluation of ClpP as a vaccine target in Streptococcus pneumoniae	5
Enzymatic synthesis of inositol plasmalogens from distinct lipid sources using phospholipase D from Streptomyces antibioticus	5
Quantitative analysis of genome truncation patterns in oversized adeno-associated virus vectors	5
Exploring potential bacterial populations for enhanced anthraquinone dyes biodegradation: a critical review	5
Identification of antifungal metabolites from a soil-derived Streptomyces strain active against Fusarium graminearum using genome mining	5
Biogenesis and characterization of proficient silver nanoparticles employing marine procured fungi Hamigera pallida and assessment of their antioxidative, antimicrobial and anticancer potency	5
ANCUT1, a novel thermoalkaline cutinase from Aspergillus nidulans and its application on hydroxycinnamic acids lipophilization	5
Recombinant AcMNPV-gp64-EGFP and synergist triphenyl phosphate, an effective combination against Spodoptera frugiperda	5