OOIR: Observatory of International Research

Papers

(The TQCC of Biotechnology Letters is 5. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-10-01 to 2025-10-01.)

Article	Citations
Influence of affinity tags and tobacco PR1a signal peptide on detection, purification and bioactivity analyses of the small oomycete apoplastic effectors	105
Anaerobic biofilm enriched with an ammonia tolerant methanogenic consortium to improve wastewater treatment in the fishing industry	44
Cell density detection based on a microfluidic chip with two electrode pairs	40
Activation of galactose utilization by the addition of glucose for the fermentation of agar hydrolysate using Lactobacillus brevis ATCC 14869	40
Recombinant expression, downstream optimization, and therapeutic evaluation of recombinant human interleukin-37 for cancer therapy	32
Listeria monocytogenes is a solvent tolerant organism secreting a solvent stable lipase: potential biotechnological applications	30
Unraveling the molecular mechanisms of selenite reduction: transcriptomic analysis of Bacillus reveals the key role of sulfur assimilation	29
Enhancement of riboflavin production in Bacillus subtilis via in vitro and in vivo metabolic engineering of pentose phosphate pathway	29
The pedal-like loop of (R)-selective transaminases plays a critical role to the functionality of the enzyme	29
Directed evolution of cholesterol oxidase with improved thermostability using error-prone PCR	26
Silica nanoparticles enhance interfacial self-adherence of a multi-layered extracellular matrix scaffold for vascular tissue regeneration	23
Synthesis of β-ionone from xylose and lignocellulosic hydrolysate in genetically engineered oleaginous yeast Yarrowia lipolytica	21
Efficient immobilization behavior and mechanism investigation of Pb(II) by Aspergillus tubingensis	19
Factors affecting rAAV titers during triple-plasmid transient transfection in HEK-293 cells	19
Synergistic anticancer effects of interleukin-21 combined with therapeutic peptides in multiple cancer cells	17
Squalene hopene cyclases and oxido squalene cyclases: potential targets for regulating cyclisation reactions	17
Design and application of expression constructs for FMDV serotype O structural proteins	17
Application of lactose based autoinduction for heterologous production of an active [NiFe] hydrogenase in E. coli	16
High throughput screening and metabolic engineering of Saccharopolyspora spinosa for spinosad production	15
Application of exogenous melatonin in vitro and in planta: a review of its effects and mechanisms of action	14
Paenibacillus barengoltzii: isolation, growth, and characterization of a high dextrinizer α-amylase	14
Identification of laccase genes in Trichoderma asperellum Ts93	13
Metabolic response of Bacillus spp. to heavy metal stress: pathway alterations and metabolite profiles	13
Retraction Note: Autophagy regulated by lncRNA HOTAIR contributes to the cisplatin-induced resistance in endometrial cancer cells	13
Biocontrol of strawberry Botrytis gray mold and prolong the fruit shelf-life by fumigant Trichoderma spp.	13

Retraction Note: TP53INP1 3′-UTR functions as a ceRNA in repressing the metastasis of glioma cells by regulating miRNA activity	12
Therapeutic potential of a novel IP-10-(anti-HER2 scFv) fusion protein for the treatment of HER2-positive breast cancer	12
Clinical and mechanistic insights into biomedical application of Se-enriched probiotics and biogenic selenium nanoparticles	12
BMSCs improve TNBS-induced colitis in rats by inducing Treg differentiation by expressing PD-L1	12
Molecular cloning and modelling of a thermostable α-amylase from a thermophilic Geobacillus sp. DS3	12
Enhancing lipase activity in Aspergillus niger through CRISPR/Cas9-mediated protease gene knockout and fermentation optimization	12
Calumenin knockdown, by intronic artificial microRNA, to improve expression efficiency of the recombinant human coagulation factor IX	11
Enhancement hispolon production from Phellinus linteus via epigenetic-modified culture to inhibit human breast cancer cells	11
Isolation, microscopic and magnetotactic characterization of Magnetospirillum moscoviense MS-24 from Banjosa Lake, Pakistan	11
Optimizing hyaluronic acid production by Streptococcus zooepidemicus using taguchi method: effects of temperature and pH	11
Research on the application of heterotrophic nitrification-aerobic denitrification bacteria in membrane bioreactor (MBR)	10
Magnetic particle-based chemiluminescence immunoassay for serum human heart-type fatty acid binding protein measurement	10
Combining biotechnology with sustainability: feasibility of bioenergy sorghum in generating high-value bioproducts	10
The modification of heme special importer to improve the production of active hemoglobins in Escherichia coli	10
Succession from acetoclastic to hydrogenotrophic microbial community during sewage sludge anaerobic digestion for bioenergy production	10
Substrate type and CO2 addition significantly influence succinic acid production of Basfia succiniciproducens	10
Micro-electrochemical DO sensor with ultra-micropore matrix fabricated with femtosecond laser processing successfully applied in on-line DO monitoring for yeast culture	10
Gallic acid as biofilm inhibitor can improve transformation efficiency of Ruminiclostridium papyrosolvens	10
Role of microbial pesticides in pest control for lepidopteran: research status and prospect	10
Exploration of a three-dimensional matrix as micro-reactor in the form of reactive polyaminosaccharide hydrogel beads using multipoint covalent interaction approach	9
Bone marrow mesenchymal stem cell-derived exosomes loaded with miR-26a through the novel immunomodulatory peptide DP7-C can promote osteogenesis	9
Transcriptome expression profiles reveal response mechanisms to drought and drought-stress mitigation mechanisms by exogenous glycine betaine in maize	9
Construction of a bacteriophage-derived vector with potential applications in targeted drug delivery and cell imaging	9
Application of low-intensity ultrasound to enhance simultaneous nitrification/iron-based autotrophic denitrification	9
An artificial multienzyme cascade for the whole-cell synthesis of rare ketoses from glycerol	9
Development of a synthetic transcription factor-based S-adenosylmethionine biosensor in Saccharomyces cerevisiae	8
Cellulase and chitinase activities and antagonism against Fusarium oxysporum f.sp. cubense race 1 of six Trichoderma strains isolated from Mexican maize cropping	8
Aptamer-functionalized MOFs and AI-driven strategies for early cancer diagnosis and therapeutics	8
Investigation of osteogenesis and angiogenesis in perfusion bioreactors using improved multi-layer PCL-nHA-nZnO electrospun scaffolds	8
Identification, molecular and biochemical characterization of a novel thermoactive and thermostable glucoamylase from Thermoanaerobacter ethanolicus	8
Measurement of HbA1c with a new phase function using RBC phase image from a low-cost digital holographic microscopy	8
Toxicants improve glycerol production in the fermentation of undetoxified hydrolysate by Candida glycerinogenes	8
The variation of microorganisms and organics during methane production from lignite under an electric field	8
Trichoderma longibrachiatum and thermothelomyces thermophilus co-culture: improvement the saccharification profile of different sugarcane bagasse varieties	8
Characterization of a novel Escherichia coli recombineering selection/counterselection cassette	8
Application of CRISPR-Cas9 in microbial cell factories	8
Improving the specific activity and stability of alkaline pectinase PEL3 through SpyTag/SpyCatcher cyclization	8
Novel TdsD nitroreductase: characterization of kinetics and substrate specificity	8
Differentiation, maturation, and collection of THP-1-derived dendritic cells based on a PEG hydrogel culture platform	8
Mutational analysis of pig tissue factor pathway inhibitor α to increase anti-coagulation activity in pig-to-human xenotransplantation	8
Cost-effective production of squalene using Yarrowia lipolytica via metabolic engineering and fermentation engineering	7
Establishment of a visual gene knockout system based on CRISPR/Cas9 for the rare actinomycete Nonomuraea gerenzanensis	7
Biotransformation of C20- and C22-polyunsaturated fatty acids to 11S- and 13S-hydroxy fatty acids by Escherichia coli expressing 11S-lipoxygenase from Enhygromyxa salina	7
Optimizing microbial electrolysis cell performance: strategies to mitigate electron mediator degradation on membranes	7
Genome-scale metabolic model-based engineering of Escherichia coli enhances recombinant single-chain antibody fragment production	7
Enhancement of cordycepin production from Cordyceps militaris culture by epigenetic modification	7
Competitive inhibition of a non-natural cofactor dependent formaldehyde dehydrogenase by imidazole	7
Expansion of YALIcloneHR toolkit for Yarrowia lipolytica combined with Golden Gate and CRISPR technology	7
Carrier immobilization and auto-exposition favoring reuse of silyletherase SilE-R from Brassica sp. with high activity and enantiospecificity	7
Optimization of ultrasound-assisted production of ergosterol from Penicillium brevicompactum by Taguchi statistical method	7

Preparation of white rot fungal inoculum and its application to bioremediation of chlorimuron-ethyl-contaminated soil	7
Dynamic cultivation of human mesenchymal stem/stromal cells for the production of extracellular vesicles in a 3D bioreactor system	7
Effects of the deletion and substitution of thioesterase on bacillomycin D synthesis	7
Isolation and characterisation of exosomes from Chinese hamster ovary (CHO) cells	7
Current advances and future prospects in production of recombinant insulin and other proteins to treat diabetes mellitus	7
A gold nanoparticles-based lateral flow assay utilizing baculovirus expressed recombinant nucleocapsid and receptor binding domain proteins for serodetection of IgG and IgM against SARS-CoV-2	7
Improving the hydrophilic microenvironment surrounding the catalytic site of fructosyltransferase enhances its catalytic ability	7
A novel P450 enzyme assay utilizing an NADP+-based biosensor	7
Economic optimization of expression of soluble human epidermal growth factor in Escherichia coli	7
Engineering a synthesis-friendly serum responsive promoter for antibiotic selection of genomic integration in cell-based therapy	7
Enhancing HAP3 mediated xylose metabolism and acetic acid metabolism promotes lipid production from lignocellulosic hydrolysate fermented by Candida glycerinogenes	7
Roles of PKAc1 and CRE1 in cellulose degradation, conidiation, and yellow pigment synthesis in Trichoderma reesei QM6a	6
Homo- and heterofermentative lactobacilli are distinctly affected by furanic compounds	6
ANCUT1, a novel thermoalkaline cutinase from Aspergillus nidulans and its application on hydroxycinnamic acids lipophilization	6
Advanced biotechnology using methyltransferase and its applications in bacteria: a mini review	6
A cell-free fluorescence biosensor based on allosteric transcription factor NalC for detection of pentachlorophenol	6
Antimicrobial activity of thermophilin 110 against the opportunistic pathogen Cutibacterium acnes	6
Exploring potential bacterial populations for enhanced anthraquinone dyes biodegradation: a critical review	6
RETRACTED ARTICLE: Characterization of a new bacteriocin-like inhibitory peptide produced by Lactobacillus sakei B-RKM 0559	6
Biogenesis and characterization of proficient silver nanoparticles employing marine procured fungi Hamigera pallida and assessment of their antioxidative, antimicrobial and anticancer potency	6
Recombinant protein CP01850 adjuvanted with Iridea cordata or Sacorpletis skottibergii lipid extracts protected mice against infection by Corynebacterium pseudotuberculosis	6
A single-vector CRISPR/Cas9 system for genome editing and heterologous enzyme secretion in Saccharomyces cerevisiae: a case study on pectate lyase for coffee mucilage removal	6
Correction to: Multilineage differentiation of muscle-derived stem cells from GFP transgenic mice	6
Pilot-scale production of Bacillus subtilis MSCL 897 spore biomass and antifungal secondary metabolites in a low-cost medium	6
Enhanced soluble expression of active recombinant human interleukin-29 using champion pET SUMO system	6
GH2 family β-galactosidases evolution using degenerate oligonucleotide gene shuffling	6
Identification of antifungal metabolites from a soil-derived Streptomyces strain active against Fusarium graminearum using genome mining	6
Efficient and high-density immobilization of animal cells by a microfiber with both swelling and cell adhesion properties and its application to exosome production	6
Development of an animal-derived component-free medium for Spodoptera frugiperda (Sf9) cells using response surface methodology	6
Molecular cloning and characterization of an alpha-amylase inhibitor (TkAAI) gene from Trichosanthes kirilowii Maxim.	6
Correction: Polyhydroxyalkanoate production by Paramecium caudatum isolated from industrial wastewater: a micro eukaryotic host for bioplastic accumulation	6
Recombinant AcMNPV-gp64-EGFP and synergist triphenyl phosphate, an effective combination against Spodoptera frugiperda	5
Antigenic and conserved peptides from diverse Helicobacter pylori antigens	5
Anti-inflammatory potential of baicalein combined with silk fibroin protein in a zebrafish model (Danio rerio)	5
WAVE-based intensified perfusion cell culture for fast process development	5
Characterization of the pesticidal crystal toxin protein Cry11Aa from Bacillus thuringiensis serovar israelensis VCRC B646 for mosquito larvae control	5
Integrated bioinformatics based subtractive genomics approach to decipher the therapeutic function of hypothetical proteins from Salmonella typhi XDR H-58 strain	5
Production enhancement of the glycopeptide antibiotic A40926 by an engineered Nonomuraea gerenzanensis strain	5
Antibiotic-free production of sucrose isomerase in Bacillus subtilis by genome integration	5
An inducible Komagataella phaffii system for protein expression using sorbitol dehydrogenase promoter	5
Monitoring of a microbial community during bioaugmentation with hydrogenotrophic methanogens to improve methane yield of an anaerobic digestion process	5
Redifferentiation of genetically modified dedifferentiated chondrocytes in a microcavitary hydrogel	5
Supplementation of articular cartilage-derived chondroprogenitors with bone morphogenic protein-9 enhances chondrogenesis without affecting hypertrophy	5
Citrus essential oil: would it be feasible as antimicrobial in the bioethanol industry?	5
From yeast screening for suitability as single cell protein to fed-batch cultures	5
Influence of 16S rRNA reference databases in amplicon-based environmental microbiome research	5
Completely noninvasive multi-analyte monitoring system for cell culture processes	5
De novo biosynthesis of 2′-fucosyllactose in engineered Pichia pastoris	5
Optimization and characterization of collagenase KU665299 and its application in effective in-vitro clot digestion	5
Improving pharmacological activities of thrombopoietin mimetic peptide by genetic fusion to albumin-binding domain	5
Retraction Note: Characterization of a new bacteriocin-like inhibitory peptide produced by Lactobacillus sakei B-RKM 0559	5
CRISPR/Cas9-assisted ssDNA recombineering for site-directed mutagenesis and saturation mutagenesis of plasmid-encoded genes	5
swnk plays an important role in the biosynthesis of swainsonine in Metarhizium anisopliae	5
Enzymatic synthesis of inositol plasmalogens from distinct lipid sources using phospholipase D from Streptomyces antibioticus	5
Effect of SpyTag/SpyCatcher cyclization on stability and refolding of green fluorescent protein	5
Assessment of population structure, genetic diversity and relationship of Mediterranean olive accessions using SSR markers and computational tools	5
Synthesis of di-rhamnolipids by the avirulent, mono-rhamnolipid producing strain Pseudomonas aeruginosa ATCC 9027	5
A novel thermophilic β-carotene 15,15′-monooxygenase with broad substrate specificity from the marine bacterium Candidatus Pelagibacter sp. HTCC7211	5
Improvement of transglutaminase production by controlling the morphology of Streptomyces mobaraensis HVCP-Sm1 with microparticle-enhanced cultivation	5