Journal of Virological Methods

Article	Citations
Loop-Mediated Isothermal Amplification assays for on-site detection of the main sweetpotato infecting viruses	86
Comparison of in-house SARS-CoV-2 genome extraction procedures. A need for COVID-19 pandemic	78
Development and performance evaluation of the first in-house multiplex rRT-PCR assay in Bangladesh for highly sensitive detection of SARS-CoV-2	77
Enhancing lumpy skin disease control: Effective competitive and indirect ELISAs for serological surveillance	57
A multiplex one-step fluorescence quantitative differential diagnosis method for severe hand, foot and mouth disease caused by coxsackievirus A16	42
Metagenomics in the fight against zoonotic viral infections: A focus on SARS-CoV-2 analogues	40
Potential use of high-resolution melting analyses for SARS-CoV-2 genomic surveillance	40
Endothelial growth media components alter SARS-CoV-2 spike-directed growth kinetics	40
Editorial Board	34
Editorial Board	32
Diagnostic evaluation of Panbio™ antigen rapid diagnostic test for SARS‐CoV‐2: A systematic review and meta-analysis	32
Development of a high-throughput flow cytometric neutralization assay to screen for human enterovirus A71 (EVA71) neutralizing antibodies	31
The comparative study revealed that the hTERT-CSF cell line was the most susceptible cell to the Lumpy skin disease virus infection among eleven cells	30
Editorial Board	28
A luciferase-based approach for measuring HBGA blockade antibody titers against human norovirus	26
Development of a double-antibody sandwich enzyme-linked immunosorbent assay for rapid detection of VZV	26
Editorial Board	25
Characterization of murine cytomegalovirus infection and induction of calcification in Murine Aortic Vascular Smooth Muscle Cells (MOVAS)	23
Improvement in the sensitivity of viroid detection by adapting the reverse transcription step in one-step RT-qPCR assays	23
Investigating the sequence variation in the influenza A matrix genes during the 2017-2018 and 2018-2019 seasons in samples from a local population in London	23
Development of a new Collateral Cleavage-independent CRISPR/Cas12a based easy detection system for plant viruses	22
Characterization and use in neutralization assays of avian influenza codon-optimized H5 and H7 retroviral pseudotypes	22
A multiplex RT-PCR assay combined with co-extraction of DNA and RNA for simultaneous detection of TYLCV and ToCV in whitefly	21
A comparative study of pathogen inactivation technologies in human platelet lysate and its optimal efficiency in human placenta-derived stem cells culture	21
Performance of RT-qPCR detection of SARS-CoV-2 in unextracted nasopharyngeal samples using the Seegene Allplex 2019-nCoV protocol	21

Evaluation of sample pooling using the SAMBA II SARS-CoV-2 test	21
Editorial Board	20
Recombinase polymerase amplification assay for sensitive and rapid detection of southern rice black-streaked dwarf virus in plants	19
Editorial Board	18
Evaluation of a simple ultrafiltration method for concentration of infective canine parvovirus and feline coronavirus from cell culture supernatants	18
Nervous necrosis virus titration and antigen quantitation by indirect sandwich enzyme linked immunosorbent assay	18
A replication competent luciferase-secreting DENV2 reporter for sero-epidemiological surveillance of neutralizing and enhancing antibodies	18
Editorial Board	18
Amino acid variations of the immuno-dominant domain of respiratory syncytial virus attachment glycoprotein (G) affect the antibody responses In BALB/c mice	18
Editorial Board	18
The enhancement role of Matrigel on HBV infection in HepG2-NTCP cells	17
Comparison of FTD SARS-CoV-2 Assay and RealStar RT-PCR kit 1.0 for the detection of SARS-CoV-2	17
Detection of a characteristic melting profile of a SARS-CoV-2 Kappa variant in Italy using the SARS-CoV-2 Variants ELITe MGB® Kit	17
Development and validation of a DIVA ELISA for differentiating BEFV infected from vaccinated animals	17
Specific detection of Malus- and Pyrus-infecting viroids by real-time reverse-transcription quantitative PCR assays	17
SARS-CoV-2-specific T cell responses: a comparative analysis between QuantiFERON SARS-CoV-2, T-SPOT.COVID, and an in-house Omicron ELISpot	16
Automated, label-free TCID50 assay to determine the infectious titer of virus-based therapeutics	16
Investigation of atypical serological profiles for Epstein-Barr virus (EBV)	16
Digital PCR assay for the effective detection of COVID-19 patients with SARS-CoV-2 low viral load	16
May viral load detected in saliva in the early stages of infection be a prognostic indicator in COVID-19 patients?	16
Design, development, and validation of a strand-specific RT-qPCR assay to differentiate replicating versus nonreplicating Rabies virus	15
Evaluation of a surrogate virus neutralization test for high-throughput serosurveillance of SARS-CoV-2	15
Reverse transcription recombinase polymerase amplification assay for rapid detection of the cucurbit chlorotic yellows virus	15
Editorial Board	15
Evaluation of antigen-based rapid detection test for the diagnosis of SARS CoV-2 in low-income countries	15
Climatic determinants of monkeypox transmission: A multi-national analysis using generalized count mixed models	15
A double-strain TM (gp45) polypeptide antigen and its application in the serodiagnosis of equine infectious anemia	15
Editorial Board	15
The use of human intestinal enteroid cell cultures for detection of multiple gastroenteric viruses	15
Evaluation of the Alinity m CMV assay for detecting and quantifying cytomegalovirus DNA in non-plasma samples	15
Cell-based reporter assays for measurements of antibody-mediated cellular cytotoxicity and phagocytosis against SARS-CoV-2 spike protein	14
Comparison of eleven in vitro diagnostic assays for the detection of SARS-CoV-2 RNA	14
RT-qPCR genotyping assays for differentiating Rift Valley fever phlebovirus strains	14
A one-step real-time RT-PCR assay for simultaneous typing of SARS-CoV-2 mutations associated with the E484K and N501Y spike protein amino-acid substitutions	13
Development of a multiplex RT-PCR assay for the detection of soybean mosaic virus, bean common mosaic virus and cucumber mosaic virus in field samples of soybean	13
The Diagnostic Accuracy of Xpert Xpress to SARS-CoV-2: A systematic review	13
Fast detection of SARS-CoV-2 variants including Omicron using one-step RT-PCR and Sanger sequencing	13
Development and validation of a multiplex reverse transcriptase-PCR assay for simultaneous testing of influenza A, influenza B and SARS-CoV-2	13
Cross comparison of AmpFire HPV genotyping assay and Roche human papillomavirus (HPV) linear array for HPV genotyping of anal swab samples	12
Development and analytical validation of a novel quantitative PCR assay for the detection of Trachemys herpesvirus 1	12
Resistance of poliovirus 1 and enterovirus A71 against alcohol and other disinfectants	12
Evaluation of sensitivity and specificity in RNA-Seq-based detection of grapevine viral pathogens	12
Reducing amplification cycles to improve the coverage of influenza A virus genome sequencing in heterosubtypic co-infection	12
Multiplex qPCR development for the simultaneous and rapid detection of largemouth bass virus and infectious spleen and kidney necrosis virus in aquaculture	12
Evaluation of Illumina® COVIDSeq™ as a tool for Omicron SARS-CoV-2 characterisation	12
A method for screening CDV microneutralization activity in microvolume samples	11
Simultaneous detection of SARS-CoV-2 and identification of spike D614G mutation using point-of-care real-time polymerase chain reaction	11
Development of a microneutralization assay for HSV-2	11
Purification of hepatitis C virus core protein in non-denaturing condition	11
The development of a rapid, high-throughput neutralization assay using a SARS-CoV-2 reporter	11

Evaluation of the impact of pre-analytical conditions on sample stability for the detection of SARS-CoV-2 RNA	11
Infectivity titers and aggregation states of intracellular and extracellular nervous necrosis virus in cell lines with cytolytic and persistent infections	11
Development of a recombinant ELISA for ovine herpesvirus 2, suitable for use in sheep	11
Standardization of a high-performance RT-qPCR for viral load absolute quantification of influenza A	11
Development and evaluation of a monoclonal antibody-based blocking ELISA to detect antibodies against the E2 protein of bovine viral diarrhea virus-1	11
Differential detection of zika virus based on PCR	11
Rapid titration of recombinant baculoviruses based on NanoLuc secretion in early infection	11
An indirect ELISA to detect antibodies to the gC of bovine alphaherpesvirus 1 (BoAHV1) displaying no crossreactivity with antibodies induced by bovine alphaherpesvirus 5 (BoAHV5)	11
Rapid reverse transcriptase recombinase polymerase amplification assay for flaviviruses using non-infectious in vitro transcribed RNA as positive controls	11
Exocellular polysaccharides extracted from mangrove fungus Paecilomyces Lilacinuson present anti-HSV-1 activity in mice	11
One-step TaqMan® RT-qPCR detection of sugar beet-infecting poleroviruses in Myzus persicae from yellow water pan traps opens up new possibilities for early risk assessment of virus yellows disease	11
Evaluation of the analytical performance and specificity of a SARS-CoV-2 transcription-mediated amplification assay	11
Rapid and sensitive detection of high pathogenicity Eurasian clade 2.3.4.4b avian influenza viruses in wild birds and poultry	10
Comparison of quantitative PCR and digital PCR assays for quantitative detection of infectious bronchitis virus (IBV) genome	10
Development and application of monoclonal antibody-based dot-ELISA and colloidal gold immunochromatographic strip for rapid, specific, and sensitive detection of tomato brown rugose fruit virus	10
Analysis of the antigenic and immunogenic properties of the native rabies virus glycoprotein purified by Lens culinaris lectin affinity chromatography	10
Diagnostic accuracy of three SARS-CoV2 antibody detection assays, neutralizing effect and longevity of serum antibodies	10
Diminished amplification of SARS-CoV-2 ORF1ab in a commercial dual-target qRT-PCR diagnostic assay	10
Establishment of a real-time Recombinase Polymerase Amplification (RPA) for the detection of decapod iridescent virus 1 (DIV1)	10
Real-world performance of the NeuMoDx™ HCV Quant Test for quantification of hepatitis C virus (HCV)-RNA	10
Evaluation of low-cost viral concentration methods in wastewaters: Implications for SARS-CoV-2 pandemic surveillances	10
Label-free imaging of nuclear membrane for analysis of nuclear import of viral complexes	10
Evaluation of insulated isothermal PCR devices for the detection of avian influenza virus	10
In vitro evaluation of the effect of mutations in primer binding sites on detection of SARS-CoV-2 by RT-qPCR	10
Optimal conditions for adenoviral transduction of immature dendritic cells without affecting the tolerogenic activity of DC-based immunotherapy	10
A rapid competitive method for bacteriophage genomic DNA extraction	10
Development of an indirect ELISA method based on the VP4 protein for detection antibody against duck hepatitis A virus type 1	10
Effect of time and temperature on the stability of HPV and cellular nucleic acid using simulated dry self-samples	10
Rapid and sensitive detection of hepatitis B virus by lateral flow recombinase polymerase amplification assay	9
Insights into the evolutionary and prophylactic analysis of SARS-CoV-2: A review	9
Curing two predominant viruses occurring in Lentinula edodes by chemotherapy and mycelial fragmentation methods	9
Comparison of Polymerase Chain Reaction (PCR) assay performance in detecting Decapod penstylhamaparvovirus 1 in penaeid shrimp	9
Development of a T7 RNA polymerase expressing cell line using lentivirus vectors for the recovery of recombinant Newcastle disease virus	9
Clinical and analytical performance of the CLART HPV 4S assay with SurePath screening samples from the Danish cervical cancer screening program using the VALGENT framework	9
Validation of a severe acute respiratory syndrome coronavirus 2 microneutralization assay for evaluation of vaccine immunogenicity	9
Implication of cell culture methods and biases on UV inactivation of viruses	9
Prevalence and phylogenetic analysis of HTLV-1 in blood donors in Golestan Province, in the Northeast of Iran	9
Evaluation of formulations to improve SARS-CoV-2 viability and thermostability after lyophilisation	9
WITHDRAWN: Development and evaluation of an indirect ELISA based on recombinant structural protein σC to detect antibodies against avian orthoreovirus	9
Editorial Board	9
Approaches to produce and characterize recombinant protein VP1-2A of HAV for serological rapid test application	9
SARS-Cov-2 pneumonia and concurrent myelodysplasia complicated by Pseudomonas aeruginosa over-infection	9
Mouse hepatitis virus: A betacoronavirus model to study the virucidal activity of air disinfection equipment on surface contamination	9
Detection of cytomegalovirus in bronchoalveolar lavage fluid from immunocompromised patients with pneumonitis by viral culture and DNA quantification	9
Development of a multiplex real-time quantitative reverse-transcription polymerase chain reaction for the detection of four bee viruses	9
Development and validation of a duplex RT-qPCR assay for norovirus quantification in wastewater samples	9
Discrepant results of hepatitis B virus genotype determination by PCR and DNA sequencing	9
Screening and identification of B cell epitope within the major capsid protein L1 of HPV 52, using monoclonal antibodies	9
Rapid identification of SARS-CoV-2 variants: Validation of the simplexa SARS-CoV-2 variant direct assay	9
Visualizing intracellular sialidase activity of influenza A virus neuraminidase using a fluorescence imaging probe	9
Avidin-Biotin recombinant antigen capture ELISA for the detection of peste des petits ruminants virus in the clinical specimens of sheep and goats	9
Establishment of a duplex TaqMan RT-PCR for the differential detection of RHDV GI.1 and GI.2	9
Tween 80 improves the infectivity of BCL1 cell-adapted infectious bursal disease virus	8
Reduced amplification efficiency of the RNA-dependent-RNA-polymerase target enables tracking of the Delta SARS-CoV-2 variant using routine diagnostic tests	8
Assessment of SARS-CoV-2 viral loads in combined nasal-and-throat swabs collected from COVID-19 individuals under the Universal Community Testing Programme in Hong Kong	8
Development and applications of a TaqMan based quantitative real-time PCR for the rapid detection of Pigeon circovirus (PiCV)	8
Diagnosis of Monkeypox infection: Validation of two diagnostic kits for viral detection using RT-PCR	8
Efficiency of CMV serodiagnosis during pregnancy in daily laboratory routine	8
Performance of the Roche IL-6 chemiluminescent immunoassay in patients with COVID-like respiratory symptoms	8
Development and application of a visual microarray for synchronously detecting H5N1, H7N9 and H9N2 avian influenza virus RNA	8
Development of a chronic focal equine arteritis virus infection of a male reproductive tract cell line	8
Concordance of Anyplex™ II HPV HR assays with reference HPV assays in cervical cancer screening: Systematic review	8
Development of colloidal gold-based test strip for rapid detection of serotype 4 fowl adenovirus	8
Detection and absolute quantitation of watermelon mosaic virus by real-time RT-PCR with a TaqMan probe	8
A thermal cycler-based, homogenization-free plant total nucleic acid extraction method for plant viruses and viroids assay	8
Generation and application of immortalized sertoli cell line from sheep testis	8
Detection of Sri Lankan cassava mosaic virus by loop-mediated isothermal amplification using dried reagents	8
Simultaneous detection of five viruses and two viroids affecting apples through a DNA macroarray chip	8
Detection and quantification of antibody to SARS CoV 2 receptor binding domain provides enhanced sensitivity, specificity and utility	8
Increasing diagnostic possibilities using the geneLEAD VIII platform for detection of SARS-CoV-2	8
Generation of an A549 ISRE-luciferase stable cell line	8
Potentialities of a mesoporous activated carbon as virus detection probe in aquatic systems	8
Development of a sensitive, multi-assay platform to monitor low levels of HBV DNA and pgRNA in patients with chronic hepatitis B virus infection	8
Alternative RNA extraction-free techniques for the real-time RT-PCR detection of SARS-CoV-2 in nasopharyngeal swab and sputum samples	8
Comparison of four PCR and two point of care assays used in the laboratory detection of SARS-CoV-2	8
Fully automated dried blood spot sample handling and extraction for BoHV-1 antibody testing by ELISA	8
Editorial Board	7
COVID-19 in Hong Kong – Public health, food safety, and animal vectors perspectives	7
Corrigendum to “Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections” J. Virol. Methods 206 (2014) 38–41	7
A duplex SYBR green I-based real-time polymerase chain reaction assay for concurrent detection of feline parvovirus and feline coronavirus	7

An integrated cell culture reverse transcriptase quantitative PCR (ICC-RTqPCR) method to simultaneously quantify the infectious concentrations of eight environmentally relevant enterovirus serotypes	7
Generation and characterization of luciferase-secreting, single-round infectious DENV-2 reporter for functional antibody assays	7
Comparative study between virus neutralisation testing and other serological methods detecting anti-SARS-CoV-2 antibodies in Europe, 2021	7
Quantitative and qualitative analysis of seroconversion after one year of vaccination with inactivated SARS-CoV-2 vaccine (CoronaVac®) in healthcare workers: Cross-sectional analytical study	7
Development of a multiplex real-time PCR assay for the simultaneous detection of mpox virus and orthopoxvirus infections	7
The challenge of screening SARS-CoV-2 variants of concern with RT-qPCR: One variant can hide another	7
Development of a duplex TaqMan real-time RT-PCR assay for simultaneous detection of goose astrovirus genotypes 1 and 2	7
Deep sequencing of hepatitis B virus using Ion Torrent fusion primer method	7
A modified high-resolution melting-based assay (HRM) to identify the SARS-CoV-2 N501Y variant	7
Performance evaluation of automated chemiluminescence immunoassay based antigen detection – Moving towards more reliable ways to predict SARS-CoV-2 infection	7
Vaccination against tick-borne encephalitis elicits a detectable NS1 IgG antibody response	7
Development of a chemiluminescence immunoassay to accurately detect African swine fever virus antibodies in serum	7
Development of a real-time RT-PCR system applicable for rapid and pen-side diagnosis of foot-and-mouth disease using a portable device, PicoGene® PCR1100	7
Heparin-mediated PCR interference in SARS-CoV-2 assays and subsequent reversal with heparinase I	7
Enhanced throughput of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) real-time RT-PCR panel by assay multiplexing and specimen pooling	7
Development of transgenic okra (Abelmoschus esculentus L. Moench) lines having RNA mediated resistance to Yellow vein mosaic virus (Geminiviridae)	7
MALDI-TOF nucleic acid mass spectrometry for simultaneously detection of fourteen porcine viruses and its application	7
Discordant performance of mpox serological assays	6
Development of a one-step RT-qPCR assay for the detection of Grapevine leafroll-associated virus 7.	6
Carboxamide and N-alkylcarboxamide additives can greatly reduce non specific amplification in Loop-Mediated Isothermal Amplification for Foot-and-Mouth disease Virus (FMDV) using Bst 3.0 polymerase	6
Protocol for a high titer of BaEV-Rless pseudotyped lentiviral vector: Focus on syncytium formation and detachment	6
An enrichment method for capturing mumps virus whole genome sequences directly from clinical specimens	6
Editorial Board	6
Development of a duplex real-time RT-PCR assay for the detection and identification of two subgroups of human metapneumovirus in a single tube	6
High-throughput sequencing of the porcine antibody repertoire with or without PEDV infection: A proof-of-concept study	6
Surveillance of human adenoviruses in water environments: Assessing the suitability of a locally developed quenching of unincorporated amplification signal reporters-loop-mediated isothermal amplifica	6
Type and abundance of sialic acid receptors on host cell membrane affect infectivity and viral titer of different strains of Newcastle disease virus	6
Precision and correlation of ED50 and endpoint titer method in measuring HPV vaccine immunogenicity	6
Antiviral effect of an extract from Kaempferia galanga L. rhizome in mice infected with pseudorabies virus	6
RSV-GenoScan: An automated pipeline for whole-genome human respiratory syncytial virus (RSV) sequence analysis	6
Qualitative real-time RT-PCR assay for nOPV2 poliovirus detection	6
Development of polyclonal antibodies-based serological methods and a DIG-labelled DNA probe-based molecular method for detection of the Vicia cryptic virus-M in field plants	6
Development and validation of a VP7-specific EIA for determining the potency and stability of inactivated rotavirus vaccine	6
Time series analysis and predicting COVID-19 affected patients by ARIMA model using machine learning	6
Development and application of a multiplex PCR method for the simultaneous detection of goose parvovirus, waterfowl reovirus, and goose astrovirus in Muscovy ducks	6
Inactivation efficacy of H5N1 avian influenza virus by commonly used sample preparation reagents for safe laboratory practices	6
The value of point-of-care tests for the detection of SARS-CoV-2 RNA or antigen in bronchoalveolar lavage fluid	6
Development of a recombinant vaccine containing a spike S1-Fc fusion protein induced protection against MERS-CoV in human DPP4 knockin transgenic mice	6
Development of an indirect ELISA against African swine fever virus using two recombinant antigens, partial p22 and p30	6
Use of multivariate analysis to evaluate antigenic relationships between US BVDV vaccine strains and non-US genetically divergent isolates	6
Adding yeast extract to culture medium enhances replication of the avian coronavirus infectious bronchitis virus in chicken embryo kidney cells	6
Simultaneous expression of three reporter proteins from a porcine reproductive and respiratory syndrome virus-based vector	6
A novel and cost-effective real-time RT-PCR targeting 24 nucleotides deletion to differentiate PEDV wild-type and classical attenuated vaccine strains	6
A viral metagenomic protocol for nanopore sequencing of group A rotavirus	6
Editorial Board	6
How to choose the right real-time RT-PCR primer sets for the SARS-CoV-2 genome detection?	6
Development and validation of reverse-transcription cross-priming amplification-based lateral flow assay for the detection of infectious hematopoietic necrosis virus	6
Preliminary survey of three mosquito-borne viruses using a self-established multiplex RT-qPCR assay in Chinese blood donors	6
Comparison of strain specific pathogenicity of Herpes Simplex Virus Type 1 by high-throughput sequencing	6
A new highly sensitive single-tube nested real-time PCR assay: Clinical utility in perinatal HIV-1 diagnosis	6
SpikeSeq: A rapid, cost efficient and simple method to identify SARS-CoV-2 variants of concern by Sanger sequencing part of the spike protein gene	6
A simple and high efficiency purification of His-tagged turnip yellow mosaic virus-like particle (TYMV-VLP) by nickel ion affinity precipitation	6