Trends in Biotechnology

Article	Citations
Technologies and Standardization in Research on Extracellular Vesicles	256
Plant Microbiome Engineering: Expected Benefits for Improved Crop Growth and Resilience	224
Metabolic Engineering of Escherichia coli for Natural Product Biosynthesis	223
3D Printing in Suspension Baths: Keeping the Promises of Bioprinting Afloat	182
Microbiome Engineering: Synthetic Biology of Plant-Associated Microbiomes in Sustainable Agriculture	173
High-Throughput Screening Technology in Industrial Biotechnology	172
Multiorgan-on-a-Chip: A Systemic Approach To Model and Decipher Inter-Organ Communication	171
Enabling Technologies for Personalized and Precision Medicine	167
Cold Plasma, a New Hope in the Field of Virus Inactivation	166
Grand Challenges for Industrializing Polyhydroxyalkanoates (PHAs)	150
Integrative Methods and Practical Challenges for Single-Cell Multi-omics	149
Enhancing Biopolymer Hydrogel Functionality through Interpenetrating Networks	144
Uncovering an Organ’s Molecular Architecture at Single-Cell Resolution by Spatially Resolved Transcriptomics	143
Enzyme discovery and engineering for sustainable plastic recycling	138
Phaeodactylum tricornutum: A Diatom Cell Factory	132
On-Site Therapeutic Drug Monitoring	131
Rolling Circle Replication for Biosensing, Bioimaging, and Biomedicine	131
Reap the crop wild relatives for breeding future crops	129
In situ Biogas Upgrading by CO2-to-CH4 Bioconversion	117
Protein Engineering for Improving and Diversifying Natural Product Biosynthesis	114
The Business of Cultured Meat	108
Microbial Electrosynthesis: Where Do We Go from Here?	105
Textile Triboelectric Nanogenerators for Wearable Pulse Wave Monitoring	102
Ultrafast Photonic PCR Based on Photothermal Nanomaterials	99
Advances in the Fabrication of Biomaterials for Gradient Tissue Engineering	99

From Acetate to Bio-Based Products: Underexploited Potential for Industrial Biotechnology	98
Emergence of the Stem Cell Secretome in Regenerative Engineering	95
Synthetic Biochemistry: The Bio-inspired Cell-Free Approach to Commodity Chemical Production	94
Non-Hydrolyzable Plastics – An Interdisciplinary Look at Plastic Bio-Oxidation	94
Extracellular Matrix Mimics Using Hyaluronan-Based Biomaterials	91
New Twists in Detecting mRNA Modification Dynamics	91
Gallium-Based Liquid Metal Particles for Therapeutics	90
Burkholderia: An Untapped but Promising Bacterial Genus for the Conversion of Aromatic Compounds	86
Decellularized Extracellular Matrix for Bioengineering Physiomimetic 3D in Vitro Tumor Models	85
Microbial Chassis Development for Natural Product Biosynthesis	84
Intravesical Hydrogels as Drug Reservoirs	82
Genetic Biosensor Design for Natural Product Biosynthesis in Microorganisms	80
Prospective Therapeutic Applications of Platelet Extracellular Vesicles	80
Synthetic Biology and Genome-Editing Tools for Improving PHA Metabolic Engineering	79
Metabolic Engineering of Cupriavidus necator H16 for Sustainable Biofuels from CO2	78
Bacterial Inclusion Bodies: A Treasure Trove of Bioactive Proteins	77
Animal Immunization, in Vitro Display Technologies, and Machine Learning for Antibody Discovery	76
Controlling Voltage Reversal in Microbial Fuel Cells	72
Material–Microbe Interfaces for Solar-Driven CO2 Bioelectrosynthesis	72
Biotechnological Exploration of Transformed Root Culture for Value-Added Products	68
Toward an Optimized Process for Clinical Manufacturing of CAR-Treg Cell Therapy	68
Multitechnology Biofabrication: A New Approach for the Manufacturing of Functional Tissue Structures?	66
Towards a Digital Bioprocess Replica: Computational Approaches in Biopharmaceutical Development and Manufacturing	64
Enzyme Catalyst Engineering toward the Integration of Biocatalysis and Chemocatalysis	63
THz medical imaging: from in vitro to in vivo	62
Intraoperative Bioprinting: Repairing Tissues and Organs in a Surgical Setting	62
Bioengineered 3D Models to Recapitulate Tissue Fibrosis	61
Engineered Living Materials: Taxonomies and Emerging Trends	59
Electron Storage in Electroactive Biofilms	59
Synthetic Methylotrophy: A Practical Solution for Methanol-Based Biomanufacturing	58
Developing a Computational Framework To Advance Bioprocess Scale-Up	57
Intrinsically Conductive Microbial Nanowires for ‘Green’ Electronics with Novel Functions	57
Using Microbial Aggregates to Entrap Aqueous Phosphorus	56
Microalgal Torrefaction for Solid Biofuel Production	56
Process Analytical Technologies and Data Analytics for the Manufacture of Monoclonal Antibodies	55
Combining Environmental and Economic Performance for Bioprocess Optimization	54
Microalgae-based biotechnological sequestration of carbon dioxide for net zero emissions	54
Engineered Methanotrophy: A Sustainable Solution for Methane-Based Industrial Biomanufacturing	54
Graphene for Biosensing Applications in Point-of-Care Testing	53
In Situ 3D Printing: Opportunities with Silk Inks	53
The Sustainable Path to a Circular Bioeconomy	53
Modeling the Human Body on Microfluidic Chips	53
Can Bottom-Up Synthetic Biology Generate Advanced Drug-Delivery Systems?	52
Gelatin Matrices for Growth Factor Sequestration	52
The tremendous biomedical potential of bacterial extracellular vesicles	51
Obscure yet Promising Oleaginous Yeasts for Fuel and Chemical Production	51
Sustainable Seaweed Biotechnology Solutions for Carbon Capture, Composition, and Deconstruction	51
Flexible Fungal Materials: Shaping the Future	51
Next-Generation CRISPR Technologies and Their Applications in Gene and Cell Therapy	50
Enhanced Strategies for Antibiotic Removal from Swine Wastewater in Anaerobic Digestion	50

Novel Strategies and Platforms for Industrial Isoprenoid Engineering	49
CRISPR/Cas-Based Epigenome Editing: Advances, Applications, and Clinical Utility	48
Advanced strategies and tools to facilitate and streamline microbial adaptive laboratory evolution	48
Oxygen-Releasing Biomaterials: Current Challenges and Future Applications	47
Designing Synthetic Microbial Consortia for Biofuel Production	47
Microdroplet-Assisted Screening of Biomolecule Production for Metabolic Engineering Applications	47
Engineering Microphysiological Immune System Responses on Chips	46
Host Systems for the Production of Recombinant Spider Silk	46
Creative biological lignin conversion routes toward lignin valorization	45
Microbial Coculture for Flavonoid Synthesis	44
What makes Yarrowia lipolytica well suited for industry?	44
Plant Aromatic Prenyltransferases: Tools for Microbial Cell Factories	44
Putting Photosystem I to Work: Truly Green Energy	43
Engineering the Plant Secretory Pathway for the Production of Next-Generation Pharmaceuticals	43
Hypes, hopes, and the way forward for microalgal biotechnology	43
Microbial Base Editing: A Powerful Emerging Technology for Microbial Genome Engineering	42
Synthetic Biology Approaches To Enhance Microalgal Productivity	42
Synthetic Biological Circuits within an Orthogonal Central Dogma	40
Progress toward a bicarbonate-based microalgae production system	40
Toward Deep Biophysical Cytometry: Prospects and Challenges	39
Does RNAi-Based Technology Fit within EU Sustainability Goals?	39
P450-driven plastic-degrading synthetic bacteria	39
Image-Based Live Cell Sorting	39
Single-cell mass spectrometry	39
Bioprocessing technology of muscle stem cells: implications for cultured meat	39
Recombineering for Genetic Engineering of Natural Product Biosynthetic Pathways	39
Recent Advances in the Microbial Synthesis of Hemoglobin	38
Food Waste: A Promising Source of Sustainable Biohydrogen Fuel	38
Designing Artificial Cells towards a New Generation of Biosensors	38
Innovative Tools and Strategies for Optimizing Yeast Cell Factories	37
Waste or Gold? Bioelectrochemical Resource Recovery in Source-Separated Urine	37
Production of Bio-alkanes from Biomass and CO2	37
SWATH-MS-Based Proteomics: Strategies and Applications in Plants	37
Harnessing the Power of Enzymes for Tailoring and Valorizing Lignin	37
Microbial Lipid Biotechnology to Produce Polyunsaturated Fatty Acids	36
Quality by Design for enabling RNA platform production processes	36
Pushing the rheological and mechanical boundaries of extrusion-based 3D bioprinting	36
Directed Evolution of CRISPR/Cas Systems for Precise Gene Editing	36
Bioengineering artificial blood vessels from natural materials	36
Microphysiological Systems to Recapitulate the Gut–Kidney Axis	35
Engineering Metabolism in Nicotiana Species: A Promising Future	35
Exploiting unconventional prokaryotic hosts for industrial biotechnology	35
Nano toolbox in immune modulation and nanovaccines	34
Biocatalysis in Green and Blue: Cyanobacteria	34
Innovation trends in industrial biotechnology	34
3D-bioprinted cancer-on-a-chip: level-up organotypic in vitro models	34
Emerging Argonaute-based nucleic acid biosensors	34
In situ bioprinting: intraoperative implementation of regenerative medicine	34
Microbiome-Inspired Green Infrastructure: A Toolkit for Multidisciplinary Landscape Design	33
Multiproduct biorefinery from marine thraustochytrids towards a circular bioeconomy	33
Using Co-Culture to Functionalize Clostridium Fermentation	33
Synthetic Methylotrophy in Yeasts: Towards a Circular Bioeconomy	32
Quantification of tumor heterogeneity: from data acquisition to metric generation	32
Aptamers targeting SARS-COV-2: a promising tool to fight against COVID-19	31
Magnetic Levitation Systems for Disease Diagnostics	31
Breaking the resolution limits of 3D bioprinting: future opportunities and present challenges	31
Fabricating Organized Elastin in Vascular Grafts	31
Biosensors for healthcare: current and future perspectives	31
Approaches for bacteriophage genome engineering	31
Reverse Transcriptase: From Transcriptomics to Genome Editing	30
Bioinspired materials and technology for advanced cryopreservation	30
The Formidable Challenge of Controlling High Mannose-Type N-Glycans in Therapeutic mAbs	30
Microbial lignin valorization through depolymerization to aromatics conversion	30
100 Years Later, What Is New in Glycerol Bioproduction?	29
Prime editing: advances and therapeutic applications	29
Allergenicity Assessment of Novel Food Proteins: What Should Be Improved?	29
Including Digital Sequence Data in the Nagoya Protocol Can Promote Data Sharing	29
Fifty Shades of Blue: How Blue Biotechnology is Shaping the Bioeconomy	29
Genetic engineering of the acidophilic chemolithoautotroph Acidithiobacillus ferrooxidans	29
Managing the Invasive Fall Armyworm through Biotech Crops: A Chinese Perspective	29
Phytohormone-like small biomolecules for microalgal biotechnology	29
Degradation or humification: rethinking strategies to attenuate organic pollutants	29
Advancing Mass Spectrometry Technology in cGMP Environments	29
Biomimetic 3D living materials powered by microorganisms	28
The Importance of Computational Modeling in Stem Cell Research	28
Self-Sufficient Class VII Cytochromes P450: From Full-Length Structure to Synthetic Biology Applications	28
Smart materials: rational design in biosystems via artificial intelligence	28
Linker Engineering in the Context of Synthetic Protein Switches and Sensors	27
CRISPR-Cas-mediated diagnostics	27
Machine learning in bioprocess development: from promise to practice	27

From Sequencing to Genome Editing for Cotton Improvement	27
Creating Bioethics Distance Learning Through Virtual Reality	26
Microbial protein cell factories fight back?	26
Merging automation and fundamental discovery into the design–build–test–learn cycle of nontraditional microbes	26
Centimeter-Long Microbial Electron Transport for Bioremediation Applications	26
Microvascular Fragments: More Than Just Natural Vascularization Units	26
Decellularized Scaffolds for Intervertebral Disc Regeneration	26
Microfluidics in Single-Cell Virology: Technologies and Applications	25
Carotenoid Biofortification of Crops in the CRISPR Era	25
Comparison of CRISPR/Cas and Argonaute for nucleic acid tests	25
Regulation of Metabolic Homeostasis in Cell Culture Bioprocesses	25
Renewable plant-derived lignin for electrochemical energy systems	24
Robustness: linking strain design to viable bioprocesses	24
Advances and challenges in photosynthetic hydrogen production	24
Build a Sustainable Vaccines Industry with Synthetic Biology	24
Haloarchaea, excellent candidates for removing pollutants from hypersaline wastewater	24
Discovery and delivery strategies for engineered live biotherapeutic products	24
What can biofabrication do for space and what can space do for biofabrication?	23
Adaptive Evolution Improves Algal Strains for Environmental Remediation	23
Fast biofoundries: coping with the challenges of biomanufacturing	23
Novel Modalities in DNA Data Storage	23
Progress and challenges in mass spectrometry-based analysis of antibody repertoires	23
Gene Delivery to the Skin – How Far Have We Come?	23
Discovery and mining of enzymes from the human gut microbiome	22
Biowaste upcycling into second-generation microbial protein through mixed-culture fermentation	22
Minicircle DNA: The Future for DNA-Based Vectors?	22
From Innovation to Application: Bridging the Valley of Death in Industrial Biotechnology	22
The genome editing revolution	22
Measurement Techniques to Resolve and Control Population Dynamics of Mixed-Culture Processes	22
Functional Applications of Nucleic Acid–Protein Hybrid Nanostructures	22
Metabolic engineering for sustainability and health	22
Microbial genetic engineering approach to replace shark livering for squalene	22
RNA-Cleaving NAzymes: The Next Big Thing in Biosensing?	22