Biotechnology Advances

Article	Citations
Editorial Board	515
Development of isopentenyl phosphate kinases and their application in terpenoid biosynthesis	431
Use of additives to improve collective biogas plant performances: A comprehensive review	220
Collagen processing with mesoscale aggregates as templates and building blocks	204
Halocins, natural antimicrobials of Archaea: Exotic or special or both?	187
Anaerobic digestion integrated with microbial electrolysis cell to enhance biogas production and upgrading in situ	183
Advancements of astaxanthin production in Haematococcus pluvialis: Update insight and way forward	177
New perspectives into Gluconobacter-catalysed biotransformations	154
Microbial cell surface engineering for high-level synthesis of bio-products	147
Computer-aided understanding and engineering of enzymatic selectivity	145
Recent advances in human respiratory epithelium models for drug discovery	134
Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies	129
Chiral helical scaffolds: Unlocking their potential in biomolecular interactions and biomedical applications	123
Think outside the box: 3D bioprinting concepts for biotechnological applications – recent developments and future perspectives	123
Evolving perspectives on lutein production from microalgae - A focus on productivity and heterotrophic culture	123
Opportunities for visual techniques to determine characteristics and limitations of electro-active biofilms	120
Accessing hidden microbial biosynthetic potential from underexplored sources for novel drug discovery	116
Recent advances and perspectives on production of value-added organic acids through metabolic engineering	116
Biosensors in microalgae: A roadmap for new opportunities in synthetic biology and biotechnology	113
Glycosylation in extracellular vesicles: Isolation, characterization, composition, analysis and clinical applications	110
Potential for microbial plastic degradation via assimilation of non-carbon moieties in additives	108
Phosphorylated polysaccharides: Applications, natural abundance, and new-to-nature structures generated by chemical and enzymatic functionalisation	100
Pickering emulsion biocatalysis: Bridging interfacial design with enzymatic reactions	99
Proteomic and Glyco(proteo)mic tools in the profiling of cardiac progenitors and pluripotent stem cell derived cardiomyocytes: Accelerating translation into therapy	99
Ex-situ biomethanation for CO2 valorization: State of the art, recent advances, challenges, and future prospective	93

Microbial starch debranching enzymes: Developments and applications	93
Growth-coupled high throughput selection for directed enzyme evolution	90
Editorial Board	89
Electrode functional microorganisms in bioelectrochemical systems and its regulation: A review	89
Biocatalytic oxyfunctionalization of unsaturated fatty acids to oxygenated chemicals via hydroxy fatty acids	88
Biotechnological applications of purine and pyrimidine deaminases	86
Elucidation and biosynthesis of tetrahydroisoquinoline alkaloids: Recent advances and prospects	86
Charting the landscape of RNA polymerases to unleash their potential in strain improvement	85
Unlocking the secret of lignin-enzyme interactions: Recent advances in developing state-of-the-art analytical techniques	84
From lignocellulose to plastics: Knowledge transfer on the degradation approaches by fungi	83
Methanotrophs as a reservoir for bioactive secondary metabolites: Pitfalls, insights and promises	82
Recent advances in machine learning applications in metabolic engineering	81
Genome-scale metabolic network models for industrial microorganisms metabolic engineering: Current advances and future prospects	81
Using machine learning approaches for multi-omics data analysis: A review	78
Biological valorization of lignin to flavonoids	78
Polyhydroxyalkanoates synthesis by halophiles and thermophiles: towards sustainable production of microbial bioplastics	78
Enhancing single-cell bioconversion efficiency by harnessing nanosecond pulsed electric field processing	76
Pseudomonas aeruginosa biofilms and their partners in crime	76
Emerging advances in glycoengineering of carbohydrates/glycans and their industrial applications	74
Advances in bacterial glycoprotein engineering: A critical review of current technologies, emerging challenges, and future directions	73
Fusarium as a promising fungal genus with potential application in bioremediation for pollutants mitigation: A review	72
Rutinosidase and other diglycosidases: Rising stars in biotechnology	71
Recent advances in research for potential utilization of unexplored lichen metabolites	70
Resource allocation in mammalian systems	70
Unlocking the bacterial domain for industrial biotechnology applications using universal parts and tools	70
Strategies for overcoming the limitations of enzymatic carbon dioxide reduction	70
Utilization of gene manipulation system for advancing the biotechnological potential of halophiles: A review	69
Influence of elevated pressure and pressurized fluids on microenvironment and activity of enzymes	69
Bioinspired nanoplatforms for human-machine interfaces: Recent progress in materials and device applications	66
Improving recombinant protein production in CHO cells using the CRISPR-Cas system	66
Problems and corresponding strategies for converting CO2 into value-added products in Cupriavidus necator H16 cell factories	66
Stress response in Rhodococcus strains	65
Engineering oleaginous red yeasts as versatile chassis for the production of oleochemicals and valuable compounds: Current advances and perspectives	65
Expanding sugar alcohol industry: Microbial production of sugar alcohols and associated chemocatalytic derivatives	64
From lignocellulosic biomass to single cell oil for sustainable biomanufacturing: Current advances and prospects	64
Research progress of engineering microbial cell factories for pigment production	63
C-type lectin-(like) fold – Protein-protein interaction patterns and utilization	63
Editorial Board	63
Cytoskeleton as a roadmap navigating rhizobia to establish symbiotic root nodulation in legumes	62
Synthetic biology approaches to improve tolerance of inhibitors in lignocellulosic hydrolysates	62
Recent advances in engineering synthetic biomolecular condensates	61
Biological conversion of carbon monoxide and hydrogen by anaerobic culture: Prospect of anaerobic digestion and thermochemical processes combination	61
Capturing the third dimension in drug discovery: Spatially-resolved tools for interrogation of complex 3D cell models	60
Objectives, benefits and challenges of bioreactor systems for the clinical-scale expansion of T lymphocyte cells	60
Enzyme co-immobilization: Always the biocatalyst designers' choice…or not?	59
Engineering membrane architecture for biotechnological applications	59
Recent advances in enzyme-enhanced immunosensors	59
Recent advances in lipid metabolic engineering of oleaginous yeasts	59
Genetic engineering of bacteriophages: Key concepts, strategies, and applications	59
Detection, production, modification, and application of arylsulfatases	59

Is it possible to shape the microalgal biomass composition with operational parameters for target compound accumulation?	58
Corrigendum to “Recent advances in enzyme-enhanced immunosensors” [Biotechnology Advances 53 (2021) 107867].	58
Ex vivo T cell differentiation in adoptive immunotherapy manufacturing: Critical process parameters and analytical technologies	56
Editorial Board	56
One, two, many: Strategies to alter the number of carbohydrate binding sites of lectins	54
Principles of antibodies with ultralong complementarity-determining regions and picobodies	54
Heterologous production of cellulose- and starch-degrading hydrolases to expand Saccharomyces cerevisiae substrate utilization: Lessons learnt	53
Maximizing the potential of nitrilase: Unveiling their diversity, catalytic proficiency, and versatile applications	53
Advance computational tools for multiomics data learning	53
Advances in multi-enzyme co-localization strategies for the construction of microbial cell factory	53
A reflection on the improvement of Chinese hamster ovary cell-based bioprocesses through advances in proteomic techniques	52
Conversion of inulin-rich raw plant biomass to 2,5-furandicarboxylic acid (FDCA): Progress and challenge towards biorenewable plastics	52
The technology, opportunities, and challenges of Synthetic Biological Intelligence	52
Photocatalyst-enzyme hybrid systems for light-driven biotransformation	52
Functionalized chitosan for cancer nano drug delivery	51
Interpretable and explainable predictive machine learning models for data-driven protein engineering	51
Biotechnological applications of type 1 secretion systems	50
Abiotic stress as a dynamic strategy for enhancing high value phytochemicals in microalgae: Critical insights, challenges and future prospects	50
Optimizing microbial networks through metabolic bypasses	50
Biosynthesis of monoterpenoid and sesquiterpenoid as natural flavors and fragrances	50
A guide to the use of bioassays in exploration of natural resources	49
Production of polyunsaturated fatty acids by Schizochytrium (Aurantiochytrium) spp.	49
Bacterial cellulose and its potential for biomedical applications	49
Biotechnological advances in the production of unusual fatty acids in transgenic plants and recombinant microorganisms	48
Portable biosensors for rapid on-site determination of cannabinoids in cannabis, a review	48
Transcriptomic studies of solventogenic clostridia, Clostridium acetobutylicum and Clostridium beijerinckii	48
Improving the performance of machine learning models for biotechnology: The quest for deus ex machina	48
A comprehensive and critical review on key elements to implement enzymatic PET depolymerization for recycling purposes	47
Biofilm control by interfering with c-di-GMP metabolism and signaling	47
Bacterioferritin nanocage: Structure, biological function, catalytic mechanism, self-assembly and potential applications	47
Data mining of Saccharomyces cerevisiae mutants engineered for increased tolerance towards inhibitors in lignocellulosic hydrolysates	47
Trait drift in microalgae and applications for strain improvement	47
Recent advances in tuning the expression and regulation of genes for constructing microbial cell factories	47
Rumen microbes, enzymes, metabolisms, and application in lignocellulosic waste conversion - A comprehensive review	46
Current status, and the developments of hosts and expression systems for the production of recombinant human cytokines	46
New trends in the biocatalytic production of nucleosidic active pharmaceutical ingredients using 2′-deoxyribosyltransferases	46
Metabolic and biotransformation effects on dietary glucosinolates, their bioavailability, catabolism and biological effects in different organisms	46
Advancements in nanomaterial-based aptasensors for the detection of emerging organic pollutants in environmental and biological samples	46
The Gompertz model and its applications in microbial growth and bioproduction kinetics: Past, present and future	46
Recent advances and discoveries of microbial-based glycolipids: Prospective alternative for remediation activities	46
Optogenetic technologies in translational cancer research	45
Plant glycoengineering for designing next-generation vaccines and therapeutic proteins	45
Strategies for the biological production of ectoine by using different chassis strains	45
Recent advances in the identification of biosynthetic genes and gene clusters of the polyketide-derived pathways for anthraquinone biosynthesis and biotechnological applications	45
From fungal secretomes to enzymes cocktails: The path forward to bioeconomy	45
Strategies to overcome the challenges of low or no expression of heterologous proteins in Escherichia coli	44
The isolation of lignin with native-like structure	44
Protein representations: Encoding biological information for machine learning in biocatalysis	44
Biopreservation of beer: Potential and constraints	43
Recent advances in phyto-combined remediation of heavy metal pollution in soil	43
Microbial melanin: Recent advances in biosynthesis, extraction, characterization, and applications	42
Plants arabinogalactans: From structures to physico-chemical and biological properties	42
Microbial gas fermentation technology for sustainable food protein production	42
Challenges and progress towards industrial recombinant protein production in yeasts: A review	42
CRISPR-Cas13 technology portfolio and alliance with other genetic tools	40
Controlled processivity in glycosyltransferases: A way to expand the enzymatic toolbox	40
Design and application of microfluidics in aptamer SELEX and Aptasensors	40
Ferritin nanocage based delivery vehicles: From single-, co- to compartmentalized- encapsulation of bioactive or nutraceutical compounds	39
Whole-organism phenotypic screening methods used in early-phase anthelmintic drug discovery	39
Turning waste into treasure: A new direction for low-cost production of lipid chemicals from Thraustochytrids	39
Recent advances in microbial production of diamines, aminocarboxylic acids, and diacids as potential platform chemicals and bio-based polyamides monomers	39
Engineered transcription factor-binding diversed functional nucleic acid-based synthetic biosensor	39
Fine-tuning gene expression for improved biosynthesis of natural products: From transcriptional to post-translational regulation	38
Influence of glycosylation on the immunogenicity and antigenicity of viral immunogens	38
Genetically encoded biosensors for microbial synthetic biology: From conceptual frameworks to practical applications	37
Fluorescence coupling strategies in fluorescence-activated droplet sorting (FADS) for ultrahigh-throughput screening of enzymes, metabolites, and antibodies	37
Computational advances in biosynthetic gene cluster discovery and prediction	37
HEK-omics: The promise of omics to optimize HEK293 for recombinant adeno-associated virus (rAAV) gene therapy manufacturing	37
Applications of bio-capacitance to cell culture manufacturing	37
Cupriavidus necator as a platform for polyhydroxyalkanoate production: An overview of strains, metabolism, and modeling approaches	37
Recent advances in microbial production of high-value compounds in the tetrapyrrole biosynthesis pathway	36
Using oils and fats to replace sugars as feedstocks for biomanufacturing: Challenges and opportunities for the yeast Yarrowia lipolytica	36
In vitro models of gut digestion across childhood: current developments, challenges and future trends	36
Selection of aptamers targeting small molecules by capillary electrophoresis: Advances, challenges, and prospects	36
The emerging role of cellular post-translational modifications in modulating growth and productivity of recombinant Chinese hamster ovary cells	36
From natural to synthetic: Promoter engineering in yeast expression systems	36
Designer bacterial cell factories for improved production of commercially valuable non-ribosomal peptides	35
Exploitation of lignocellulosic-based biomass biorefinery: A critical review of renewable bioresource, sustainability and economic views	35
Prospects on bio-based 2,3-butanediol and acetoin production: Recent progress and advances	35
Implications of glycosylation for the development of selected cytokines and their derivatives for medical use	34

Editorial Board	34
Refactoring transcription factors for metabolic engineering	34
Utilizing stimuli-responsive nanoparticles to deliver and enhance the anti-tumor effects of bilirubin	34
Editorial Board	34
The bioproduction of astaxanthin: A comprehensive review on the microbial synthesis and downstream extraction	34
Strategies to manipulate the performance of aptamers in SELEX, post-SELEX and microenvironment	34
Cellular variability as a driver for bioprocess innovation and optimization	34
Understanding and controlling the molecular mechanisms of protein aggregation in mAb therapeutics	34
Humans and robots: Friends of the future? A bird's eye view of biomanufacturing industry 5.0	33
Grafting of proteins onto polymeric surfaces: A synthesis and characterization challenge	33
The Yin and Yang of exosome isolation methods: conventional practice, microfluidics, and commercial kits	33
The production, recovery, and valorization of polyhydroxybutyrate (PHB) based on circular bioeconomy	33
Heparin and heparin-like modifications in hemodialysis membranes: Current innovations and future directions	32
N-oxygenation of amino compounds: Early stages in its application to the biocatalyzed preparation of bioactive compounds	32
Methods of in vitro study of galectin-glycomaterial interaction	32
Deciphering mechanisms of production of natural compounds using inducer-producer microbial consortia	32
Microbial extracellular electron transfer and strategies for engineering electroactive microorganisms	32
Progress in cyclodextrins as important molecules regulating catalytic processes of glycoside hydrolases	31
Lipopeptides development in cosmetics and pharmaceutical applications: A comprehensive review	31
Strategies for improving antimicrobial peptide production	31
Advances in the synthesis of three typical tetraterpenoids including β-carotene, lycopene and astaxanthin	31
Bacterial membrane vesicle functions, laboratory methods, and applications	31
Biopolymers and their derivatives: Key components of advanced biomedical technologies	31
Optimization strategies for CO2 biological fixation	30
Design and biocatalytic applications of genetically fused multifunctional enzymes	30
Editorial Board	30
Molecular imprinting technology for biomedical applications	30
Review of advances in the development of laccases for the valorization of lignin to enable the production of lignocellulosic biofuels and bioproducts	30
Current status and future prospects in cannabinoid production through in vitro culture and synthetic biology	30
Editorial Board	30
Yeast surface display technology: Mechanisms, applications, and perspectives	30
Relieving metabolic burden to improve robustness and bioproduction by industrial microorganisms	30
Biomass directional pyrolysis based on element economy to produce high-quality fuels, chemicals, carbon materials – A review	30
Has the time finally come for green oleochemicals and biodiesel production using large-scale enzyme technologies? Current status and new developments	30
Recent advances in synthetic biology toolkits and metabolic engineering of Ralstonia eutropha H16 for production of value-added chemicals	30
Molecular biology: Fantastic toolkits to improve knowledge and application of acetic acid bacteria	30
Editorial Board	29
Chimeric enzymes in the pulp and paper making industry: Current developments	29
Process simulation and evaluation of scaled-up biocatalytic systems: Advances, challenges, and future prospects	29
Nucleic acid-responsive smart systems for controlled cargo delivery	29
Engineering status of protein for improving microbial cell factories	29
Cell-culture process optimization via model-based predictions of metabolism and protein glycosylation	29
Antimicrobial peptides: Sustainable application informed by evolutionary constraints	29
Recent advances in fed-batch microscale bioreactor design	29
Production and characterization of starch-lignin based materials: A review	28
Identification, characterization, and engineering of glycosylation in thrombolytics	28
Next generation probiotics: Engineering live biotherapeutics	28
Insights on the emerging biotechnology of histidine-rich peptides	28
Strategies for Automated Enzymatic Glycan Synthesis (AEGS)	28
Biodegradation of polyethylene and polystyrene: From microbial deterioration to enzyme discovery	28
Mycolicibacterium cell factory for the production of steroid-based drug intermediates	28
Advanced strategies for metabolic engineering of Bacillus to produce extracellular polymeric substances	27
4D printing: Perspectives for the production of sustainable plastics for agriculture	27
Expanding the horizon of transient CAR T therapeutics using virus-free technology	27
Biophotovoltaics: Recent advances and perspectives	27
Nanoantibiotics to fight multidrug resistant infections by Gram-positive bacteria: hope or reality?	27
Role of distal sites in enzyme engineering	27
Editorial Board	27
The role of orphan crops in the transition to nutritional quality-oriented crop improvement	27
Biosynthesis of alkanes/alkenes from fatty acids or derivatives (triacylglycerols or fatty aldehydes)	26
The signaling pathways involved in metabolic regulation and stress responses of the yeast-like fungi Aureobasidium spp.	26
Extremophilic lipases for industrial applications: A general review	26
The new frontier in CHO cell line development: From random to targeted transgene integration technologies	26
Trends in the development of innovative nanobiocatalysts and their application in biocatalytic transformations	26
Engineering conditional protein-protein interactions for dynamic cellular control	26
In-depth analysis of biocatalysts by microfluidics: An emerging source of data for machine learning	25
The evolving art of creating genetic diversity: From directed evolution to synthetic biology	25
The vast repertoire of carbohydrate oxidases: An overview	25
Editorial Board	25
Editorial Board	25
Alternative biological sources for extracellular vesicles production and purification strategies for process scale-up	25
Towards efficient enzymatic saccharification of pretreated lignocellulose: Enzyme inhibition by lignin-derived phenolics and recent trends in mitigation strategies	24
Quantifying the impact of cell culture media on CHO cell growth and protein production	24
Challenges in biodegradation of non-degradable thermoplastic waste: From environmental impact to operational readiness	24
Challenges in developing cell culture media using machine learning	24
Current innovations in mRNA vaccines for targeting multidrug-resistant ESKAPE pathogens	24
Insights to improve the plant nutrient transport by CRISPR/Cas system	24
Microbial transformations by sulfur bacteria can recover value from phosphogypsum: A global problem and a possible solution	24
Industrial bioelectrochemistry for waste valorization: State of the art and challenges	24
Designing artificial pathways for improving chemical production	24
Trends in digital image processing of isolated microalgae by incorporating classification algorithm	24
Decoding cellular mechanism of recombinant adeno-associated virus (rAAV) and engineering host-cell factories toward intensified viral vector manufacturing	24
Surrogate broodstock to enhance biotechnology research and applications in aquaculture	24
Editorial Board	23
Reprint of: Advanced glycosidases as ingenious biosynthetic instruments	23
Agrobacterium strains and strain improvement: Present and outlook	23
Editorial Board	23
Biocatalytic production of biolubricants: Strategies, problems and future trends	23
Editorial Board	23
Fidelity-oriented fluorescence imaging probes for beta-galactosidase: From accurate diagnosis to precise treatment	22
Refolding in the modern biopharmaceutical industry	22
Nanobubble technologies: Applications in therapy from molecular to cellular level	22
The cell density effect in animal cell-based bioprocessing: Questions, insights and perspectives	22
A comprehensive assessment of Yarrowia lipolytica and its interactions with metals: Current updates and future prospective	22
PHA is not just a bioplastic!	22