Biotechnology Advances

Article	Citations
Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies	300
Using machine learning approaches for multi-omics data analysis: A review	288
A review of high value-added molecules production by microalgae in light of the classification	281
Microalgae as a future food source	273
Fungal potential for the degradation of petroleum-based polymers: An overview of macro- and microplastics biodegradation	235
The challenge and prospect of mRNA therapeutics landscape	221
The approved gene therapy drugs worldwide: from 1998 to 2019	217
Technologies for biological removal and recovery of nitrogen from wastewater	212
Extracellular matrix-derived biomaterials in engineering cell function	173
Enzyme co-immobilization: Always the biocatalyst designers' choice…or not?	154
Bacterial cellulose micro-nano fibres for wound healing applications	149
Lignin nanoparticles enter the scene: A promising versatile green tool for multiple applications	133
Molecular priming as an approach to induce tolerance against abiotic and oxidative stresses in crop plants	123
Microbial extracellular electron transfer and strategies for engineering electroactive microorganisms	122
Genetic engineering of microalgae for enhanced biorefinery capabilities	120
Microbial enzymes catalyzing keratin degradation: Classification, structure, function	118
Fermentation transforms the phenolic profiles and bioactivities of plant-based foods	118
Molecular farming – The slope of enlightenment	117
Microbial electrosynthesis: Towards sustainable biorefineries for production of green chemicals from CO2 emissions	114
Biotechnological potential and applications of microbial consortia	113
Biotechnological production of astaxanthin from the microalga Haematococcus pluvialis	111
Biopolymer-liposome hybrid systems for controlled delivery of bioactive compounds: Recent advances	111
Purple phototrophic bacteria for resource recovery: Challenges and opportunities	107
Recent developments in the use of peroxygenases – Exploring their high potential in selective oxyfunctionalisations	105
Mammalian cell culture for production of recombinant proteins: A review of the critical steps in their biomanufacturing	104

Algae biostimulants: A critical look at microalgal biostimulants for sustainable agricultural practices	104
Consolidated bio-saccharification: Leading lignocellulose bioconversion into the real world	103
Biodegradation and up-cycling of polyurethanes: Progress, challenges, and prospects	103
Enzymes revolutionize the bioproduction of value-added compounds: From enzyme discovery to special applications	100
Microbial melanin: Recent advances in biosynthesis, extraction, characterization, and applications	97
Potential of phytohormones as a strategy to improve microalgae productivity for biotechnological applications	97
Recent advances in systems and synthetic biology approaches for developing novel cell-factories in non-conventional yeasts	96
Adaptive laboratory evolution principles and applications in industrial biotechnology	92
Prospects and development of algal-bacterial biotechnology in environmental management and protection	88
Library preparation for next generation sequencing: A review of automation strategies	87
Glycoside hydrolase family 18 chitinases: The known and the unknown	84
Flavoprotein monooxygenases: Versatile biocatalysts	82
Biodegradation of polyethylene and polystyrene: From microbial deterioration to enzyme discovery	82
Neural tissue engineering with structured hydrogels in CNS models and therapies	82
Biological production of medium-chain carboxylates through chain elongation: An overview	80
The invisible life inside plants: Deciphering the riddles of endophytic bacterial diversity	79
Nanostructured antimicrobial peptides: The last push towards clinics	75
The Yin and Yang of exosome isolation methods: conventional practice, microfluidics, and commercial kits	74
Expanding the chemical diversity through microorganisms co-culture: Current status and outlook	74
Ecofriendly biopolymers and composites: Preparation and their applications in water-treatment	74
Lipase immobilization on ceramic supports: An overview on techniques and materials	73
Metabolic engineering of Saccharomyces cerevisiae for the production of top value chemicals from biorefinery carbohydrates	73
Strategies to manipulate the performance of aptamers in SELEX, post-SELEX and microenvironment	73
Pseudomonas aeruginosa biofilms and their partners in crime	72
Archaea Biotechnology	70
Vero cell upstream bioprocess development for the production of viral vectors and vaccines	70
Antimicrobial properties and applications of metal nanoparticles biosynthesized by green methods	69
Iron-assisted biological wastewater treatment: Synergistic effect between iron and microbes	68
Biomaterializing the promise of cardiac tissue engineering	67
Anthocyanins, multi-functional natural products of industrial relevance: Recent biotechnological advances	67
Bacterial cellulose and its potential for biomedical applications	66
Prospects on bio-based 2,3-butanediol and acetoin production: Recent progress and advances	66
Consolidated bioprocessing of raw starch to ethanol by Saccharomyces cerevisiae: Achievements and challenges	66
Developing Aspergillus niger as a cell factory for food enzyme production	65
Current trends in the production of biodegradable bioplastics: The case of polyhydroxyalkanoates	64
Current and novel approaches to downstream processing of microalgae: A review	64
Production of plant natural products through engineered Yarrowia lipolytica	63
Molecularly imprinted polymers for the selective recognition of microorganisms	62
Strategies employed in the design of antimicrobial peptides with enhanced proteolytic stability	61
Microbial production of limonene and its derivatives: Achievements and perspectives	61
Acetate as a potential feedstock for the production of value-added chemicals: Metabolism and applications	59
Biotechnological production of lipid and terpenoid from thraustochytrids	58
Inspired by nature: Microbial production, degradation and valorization of biodegradable bioplastics for life-cycle-engineered products	58
Biological and conventional food processing modifications on food proteins: Structure, functionality, and bioactivity	57
A comprehensive and critical review on key elements to implement enzymatic PET depolymerization for recycling purposes	56
Downstream processing technologies in the biocatalytic production of oligosaccharides	56
Advances in the profiling of N6-methyladenosine (m6A) modifications	55
Computational design of enzymes for biotechnological applications	55
Self-assembling peptide-based hydrogels: Fabrication, properties, and applications	54
Genetic engineering of microalgae for enhanced lipid production	54

Flavonoids, terpenoids, and polyketide antibiotics: Role of glycosylation and biocatalytic tactics in engineering glycosylation	54
Stress response in Rhodococcus strains	54
Alternative sources and metabolic engineering of Taxol: Advances and future perspectives	54
Microalgae with artificial intelligence: A digitalized perspective on genetics, systems and products	54
Challenges in biodegradation of non-degradable thermoplastic waste: From environmental impact to operational readiness	54
Biotechnological approaches for the production of natural colorants by Talaromyces/Penicillium: A review	54
Understanding gradients in industrial bioreactors	52
Review of advances in the development of laccases for the valorization of lignin to enable the production of lignocellulosic biofuels and bioproducts	52
Boosting extracellular vesicle secretion	52
A navigation guide of synthetic biology tools for Pseudomonas putida	49
How can ethanol enhance direct interspecies electron transfer in anaerobic digestion?	49
Whole-cell biocatalysis using cytochrome P450 monooxygenases for biotransformation of sustainable bioresources (fatty acids, fatty alkanes, and aromatic amino acids)	49
Sperm selection in assisted reproduction: A review of established methods and cutting-edge possibilities	49
Polyhydroxyalkanoates synthesis by halophiles and thermophiles: towards sustainable production of microbial bioplastics	48
Review: Remotely controlled magneto-regulation of therapeutics from magnetoelastic gel matrices	48
Mechanical properties of single cells: Measurement methods and applications	48
Engineering of Saccharomyces cerevisiae as a consolidated bioprocessing host to produce cellulosic ethanol: Recent advancements and current challenges	47
Bio-synthesis of food additives and colorants-a growing trend in future food	47
How does the Internet of Things (IoT) help in microalgae biorefinery?	47
Smart microalgae farming with internet-of-things for sustainable agriculture	47
Trends in the development of innovative nanobiocatalysts and their application in biocatalytic transformations	47
Biotechnological production and high potential of furan-based renewable monomers and polymers	46
On the verge of the market – Plant factories for the automated and standardized production of biopharmaceuticals	46
Algal metabolites: An inevitable substitute for antibiotics	45
Production of polyunsaturated fatty acids by Schizochytrium (Aurantiochytrium) spp.	44
Can anaerobic digestion be a suitable end-of-life scenario for biodegradable plastics? A critical review of the current situation, hurdles, and challenges	44
Ionic liquids for regulating biocatalytic process: Achievements and perspectives	44
Amidase as a versatile tool in amide-bond cleavage: From molecular features to biotechnological applications	44
What do we learn from enzyme behaviors in organic solvents? – Structural functionalization of ionic liquids for enzyme activation and stabilization	43
Unlocking the secret of lignin-enzyme interactions: Recent advances in developing state-of-the-art analytical techniques	43
Recent trends in methane to bioproduct conversion by methanotrophs	43
Next-generation metabolic engineering approaches towards development of plant cell suspension cultures as specialized metabolite producing biofactories	43
A review on the progress, challenges and prospects in commercializing microalgal fucoxanthin	42
Pathogens and predators impacting commercial production of microalgae and cyanobacteria	41
Biofilm control by interfering with c-di-GMP metabolism and signaling	41
Recent advances in lipid metabolic engineering of oleaginous yeasts	41
Biocatalytic oxidation of fatty alcohols into aldehydes for the flavors and fragrances industry	41
Biopolymers production from microalgae and cyanobacteria cultivated in wastewater: Recent advances	41
Physical methods for controlling bacterial colonization on polymer surfaces	40
From bumblebee to bioeconomy: Recent developments and perspectives for sophorolipid biosynthesis	40
Extremophilic lipases for industrial applications: A general review	40
Bacterial and enzymatic degradation of poly(cis-1,4-isoprene) rubber: Novel biotechnological applications	40
Plant synthetic biology for producing potent phyto-antimicrobials to combat antimicrobial resistance	40
Biotransformation of dietary phytoestrogens by gut microbes: A review on bidirectional interaction between phytoestrogen metabolism and gut microbiota	40
Chemical modification of enzymes to improve biocatalytic performance	39
Refactoring transcription factors for metabolic engineering	39
Expansion of human mesenchymal stem/stromal cells (hMSCs) in bioreactors using microcarriers: lessons learnt and what the future holds	39
Microbial starch debranching enzymes: Developments and applications	39
Engineer probiotic bifidobacteria for food and biomedical applications - Current status and future prospective	38
Developing fungal heterologous expression platforms to explore and improve the production of natural products from fungal biodiversity	38
Models of microalgal cultivation for added-value products - A review	38
Mycolicibacterium cell factory for the production of steroid-based drug intermediates	38
Waste-to-nutrition: a review of current and emerging conversion pathways	38
Harnessing cells to deliver nanoparticle drugs to treat cancer	38
Hot spots-making directed evolution easier	37
Marine drugs: Biology, pipelines, current and future prospects for production	37
Electro-fermentation: Sustainable bioproductions steered by electricity	36
Anodic electro-fermentation: Empowering anaerobic production processes via anodic respiration	36
Predicting drug-microbiome interactions with machine learning	36
Recent advancements and challenges in emerging applications of biochar-based catalysts	36
Current status and future prospects in cannabinoid production through in vitro culture and synthetic biology	35
Genetic control of grain appearance quality in rice	35
Insights on the emerging biotechnology of histidine-rich peptides	35
From lignocellulose to plastics: Knowledge transfer on the degradation approaches by fungi	34
Non-viral transfection technologies for next-generation therapeutic T cell engineering	34
Cyanobacteria and microalgae in supporting human habitation on Mars	34
Towards efficient enzymatic saccharification of pretreated lignocellulose: Enzyme inhibition by lignin-derived phenolics and recent trends in mitigation strategies	34
Marine macroalgae in a circular economy context: A comprehensive analysis focused on residual biomass	33
The tale of a versatile enzyme: Molecular insights into keratinase for its industrial dissemination	33
Strategies for improving antimicrobial peptide production	33
Advances in enzymatic oxyfunctionalization of aliphatic compounds	33
Engineering whole-cell microbial biosensors: Design principles and applications in monitoring and treatment of heavy metals and organic pollutants	33
Biotechnological applications of the non-conventional yeast Meyerozyma guilliermondii	33
Comparative analysis of the chemical and biochemical synthesis of keto acids	32
Adaptive defensive mechanism of bioleaching microorganisms under extremely environmental acid stress: Advances and perspectives	32
Lactic acid bacteria-derived α-glucans: From enzymatic synthesis to miscellaneous applications	32
Dehydrogenases of acetic acid bacteria	32
Xylanases of glycoside hydrolase family 30 – An overview	32
Biofilm application in the microbial biochemicals production process	32
Bacterial membrane vesicle functions, laboratory methods, and applications	31

Common problems associated with the microbial productions of aromatic compounds and corresponding metabolic engineering strategies	31
Microbial-enabled green biosynthesis of nanomaterials: Current status and future prospects	31
Microbial chassis engineering drives heterologous production of complex secondary metabolites	31
Data mining of Saccharomyces cerevisiae mutants engineered for increased tolerance towards inhibitors in lignocellulosic hydrolysates	31
Type II methanotrophs: A promising microbial cell-factory platform for bioconversion of methane to chemicals	30
Cyanobacterial sigma factors: Current and future applications for biotechnological advances	30
Phosphorylase-catalyzed bottom-up synthesis of short-chain soluble cello-oligosaccharides and property-tunable cellulosic materials	30
Lipid metabolism research in oleaginous fungus Mortierella alpina: Current progress and future prospects	30
Synthetic biology-inspired strategies and tools for engineering of microbial natural product biosynthetic pathways	30
Rhodopseudomonas palustris: A biotechnology chassis	30
Controlling biofilms using synthetic biology approaches	30
Biopolymers and their derivatives: Key components of advanced biomedical technologies	29
Opportunities and challenges of the tag-assisted protein purification techniques: Applications in the pharmaceutical industry	29
Heterologous expression of lytic polysaccharide monooxygenases (LPMOs)	29
Agrobacterium strains and strain improvement: Present and outlook	29
Protein folding and assembly in confined environments: Implications for protein aggregation in hydrogels and tissues	29
Engineering salt tolerance of photosynthetic cyanobacteria for seawater utilization	29
Towards a new avenue for producing therapeutic proteins: Microalgae as a tempting green biofactory	29
Cellular engineering strategies toward sustainable omega-3 long chain polyunsaturated fatty acids production: State of the art and perspectives	29
Engineering of primary carbon metabolism in filamentous fungi	29
Towards Green Nanoscience: From extraction to nanoformulation	29
Agarose degradation for utilization: Enzymes, pathways, metabolic engineering methods and products	29
An overview and future prospects of sialic acids	29
Noble metal nanoparticles in agriculture: impacts on plants, associated microorganisms, and biotechnological practices	29
From fungal secretomes to enzymes cocktails: The path forward to bioeconomy	29
Recent advances in fed-batch microscale bioreactor design	29
Cellulose- and xylan-degrading yeasts: Enzymes, applications and biotechnological potential	28
Synthetic protein scaffolds for the colocalisation of co-acting enzymes	28
Surrogate broodstock to enhance biotechnology research and applications in aquaculture	27
Progress in biohythane production from microalgae-wastewater sludge co-digestion: An integrated biorefinery approach	27
Bacterial sialyltransferases and their use in biocatalytic cascades for sialo-oligosaccharide production	26
Bionanomaterials based on protein self-assembly: Design and applications in biotechnology	26
Computer-aided understanding and engineering of enzymatic selectivity	26
Photocatalyst-enzyme hybrid systems for light-driven biotransformation	26
Gum polysaccharide/nanometal hybrid biocomposites in cancer diagnosis and therapy	26
Bacterial membrane transporter systems for aromatic compounds: Regulation, engineering, and biotechnological applications	26
The evolving art of creating genetic diversity: From directed evolution to synthetic biology	25
Advances in engineering UDP-sugar supply for recombinant biosynthesis of glycosides in microbes	25
Microbial inhibition and biosensing with multifunctional carbon dots: Progress and perspectives	25
CRISPR-based enrichment strategies for targeted sequencing	25
Medicinal plants used in the treatment of tuberculosis - Ethnobotanical and ethnopharmacological approaches	25
Recent advances in human respiratory epithelium models for drug discovery	25
The bacterial phylum Planctomycetes as novel source for bioactive small molecules	24
The biomedical and bioengineering potential of protein nanocompartments	24
Bacillus licheniformis: The unexplored alternative for the anaerobic production of lipopeptide biosurfactants?	24
Bacterial biofilms as platforms engineered for diverse applications	24
Advanced glycosidases as ingenious biosynthetic instruments	24
Bacterial protein acetylation and its role in cellular physiology and metabolic regulation	24
The vast repertoire of carbohydrate oxidases: An overview	24
Adaptive innovation of green plants by horizontal gene transfer	23
4D printing: Perspectives for the production of sustainable plastics for agriculture	23
Recent advances in biocatalysis of nitrogen-containing heterocycles	23
Cellular pathways of recombinant adeno-associated virus production for gene therapy	23
Immobilized fungal enzymes: Innovations and potential applications in biodegradation and biosynthesis	23
Kluyveromyces marxianus as a microbial cell factory for lignocellulosic biomass valorisation	23
Bioprospecting for bioactive compounds in microalgae: Antimicrobial compounds	23
Genetic toolkits for engineering Rhodococcus species with versatile applications	23
Nanoantibiotics to fight multidrug resistant infections by Gram-positive bacteria: hope or reality?	23
Bacterial volatile organic compounds as biopesticides, growth promoters and plant-defense elicitors: Current understanding and future scope	23
New trends in biotechnological applications of photosynthetic microorganisms	23
Insights to improve the plant nutrient transport by CRISPR/Cas system	22
Improving microalgae for biotechnology — From genetics to synthetic biology – Moving forward but not there yet	22
Advances in the synthesis of three typical tetraterpenoids including β-carotene, lycopene and astaxanthin	22
Advances in synthetic biology tools paving the way for the biomanufacturing of unusual fatty acids using the Yarrowia lipolytica chassis	22
Trends in digital image processing of isolated microalgae by incorporating classification algorithm	22
Structured edible lipid-based particle systems for oral drug-delivery	22
Mimicking natural strategies to create multi-environment enzymatic reactors: From natural cell compartments to artificial polyelectrolyte reactors	22
Factors affecting the quality of therapeutic proteins in recombinant Chinese hamster ovary cell culture	22
Resveratrol and cyclodextrins, an easy alliance: Applications in nanomedicine, green chemistry and biotechnology	22
Advances and perspectives on mass transfer and enzymatic hydrolysis in the enzyme-mediated lignocellulosic biorefinery: A review	21
Whole-organism phenotypic screening methods used in early-phase anthelmintic drug discovery	21
The signaling pathways involved in metabolic regulation and stress responses of the yeast-like fungi Aureobasidium spp.	21
Halocins, natural antimicrobials of Archaea: Exotic or special or both?	21
Recent advances in enzyme-enhanced immunosensors	21
Advances in plant-derived C-glycosides: Phytochemistry, bioactivities, and biotechnological production	21
Insertions and deletions in protein evolution and engineering	21
Recent advances in tuning the expression and regulation of genes for constructing microbial cell factories	21
Strategies for overcoming the limitations of enzymatic carbon dioxide reduction	21
Recent advances in machine learning applications in metabolic engineering	21
Genome edited wheat- current advances for the second green revolution	21
Exploration of space to achieve scientific breakthroughs	21
Metabolic engineering of microbes for monoterpenoid production	20
Paving the way towards precise and safe CRISPR genome editing	20
Insect odorant receptor-based biosensors: Current status and prospects	20
Heterologous production of cellulose- and starch-degrading hydrolases to expand Saccharomyces cerevisiae substrate utilization: Lessons learnt	20
Chromosome genomics uncovers plant genome organization and function	20
Plants arabinogalactans: From structures to physico-chemical and biological properties	20
Biomass directional pyrolysis based on element economy to produce high-quality fuels, chemicals, carbon materials – A review	20
Improvement of the enzymatic detoxification activity towards mycotoxins through structure-based engineering	20
Plant-made immunotoxin building blocks: A roadmap for producing therapeutic antibody-toxin fusions	20
Antimicrobial peptides: Promising alternatives over conventional capture ligands for biosensor-based detection of pathogenic bacteria	20
Application of microbial 3-ketosteroid Δ1-dehydrogenases in biotechnology	19
Exploitation of lignocellulosic-based biomass biorefinery: A critical review of renewable bioresource, sustainability and economic views	19
Feedback regulation and coordination of the main metabolism for bacterial growth and metabolic engineering for amino acid fermentation	19
Genetically encoded biosensors for microbial synthetic biology: From conceptual frameworks to practical applications	19