Biotechnology Advances

Article	Citations
Editorial Board	446
Development of isopentenyl phosphate kinases and their application in terpenoid biosynthesis	219
Advancements of astaxanthin production in Haematococcus pluvialis: Update insight and way forward	209
Use of additives to improve collective biogas plant performances: A comprehensive review	180
Evolving perspectives on lutein production from microalgae - A focus on productivity and heterotrophic culture	166
Halocins, natural antimicrobials of Archaea: Exotic or special or both?	153
Biotechnological production and emerging applications of betalains: A review	152
Microbial cell surface engineering for high-level synthesis of bio-products	141
New perspectives into Gluconobacter-catalysed biotransformations	135
Recent advances in human respiratory epithelium models for drug discovery	132
Collagen processing with mesoscale aggregates as templates and building blocks	130
Anaerobic digestion integrated with microbial electrolysis cell to enhance biogas production and upgrading in situ	126
Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies	123
Computer-aided understanding and engineering of enzymatic selectivity	122
Chiral helical scaffolds: Unlocking their potential in biomolecular interactions and biomedical applications	120
Accessing hidden microbial biosynthetic potential from underexplored sources for novel drug discovery	118
Think outside the box: 3D bioprinting concepts for biotechnological applications – recent developments and future perspectives	117
Recent advances and perspectives on production of value-added organic acids through metabolic engineering	110
Biosensors in microalgae: A roadmap for new opportunities in synthetic biology and biotechnology	107
Phosphorylated polysaccharides: Applications, natural abundance, and new-to-nature structures generated by chemical and enzymatic functionalisation	101
Opportunities for visual techniques to determine characteristics and limitations of electro-active biofilms	101
Glycosylation in extracellular vesicles: Isolation, characterization, composition, analysis and clinical applications	99
Pickering emulsion biocatalysis: Bridging interfacial design with enzymatic reactions	99
Alpha-ketoacid decarboxylases: Diversity, structures, reaction mechanisms, and applications for biomanufacturing of platform chemicals and fuels	98
Editorial Board	95

Elucidation and biosynthesis of tetrahydroisoquinoline alkaloids: Recent advances and prospects	93
Biotechnological applications of purine and pyrimidine deaminases	92
Enhancing single-cell bioconversion efficiency by harnessing nanosecond pulsed electric field processing	90
Biological valorization of lignin to flavonoids	90
Advances in gene editing-led route for hybrid breeding in crops	89
Methanotrophs as a reservoir for bioactive secondary metabolites: Pitfalls, insights and promises	87
Microbial starch debranching enzymes: Developments and applications	86
Electrode functional microorganisms in bioelectrochemical systems and its regulation: A review	86
Growth-coupled high throughput selection for directed enzyme evolution	85
Charting the landscape of RNA polymerases to unleash their potential in strain improvement	85
Unlocking the secret of lignin-enzyme interactions: Recent advances in developing state-of-the-art analytical techniques	83
Ex-situ biomethanation for CO2 valorization: State of the art, recent advances, challenges, and future prospective	82
Recent advances in machine learning applications in metabolic engineering	82
Haloferax and the Haloferacaceae: Potential role in bioindustry	78
Biocatalytic oxyfunctionalization of unsaturated fatty acids to oxygenated chemicals via hydroxy fatty acids	77
Genome-scale metabolic network models for industrial microorganisms metabolic engineering: Current advances and future prospects	77
From lignocellulose to plastics: Knowledge transfer on the degradation approaches by fungi	75
Polyhydroxyalkanoates synthesis by halophiles and thermophiles: towards sustainable production of microbial bioplastics	73
Emerging advances in glycoengineering of carbohydrates/glycans and their industrial applications	72
Advances in bacterial glycoprotein engineering: A critical review of current technologies, emerging challenges, and future directions	71
Bioinspired nanoplatforms for human-machine interfaces: Recent progress in materials and device applications	70
Rutinosidase and other diglycosidases: Rising stars in biotechnology	70
Fusarium as a promising fungal genus with potential application in bioremediation for pollutants mitigation: A review	69
Recent advances in research for potential utilization of unexplored lichen metabolites	69
Resource allocation in mammalian systems	69
Engineering oleaginous red yeasts as versatile chassis for the production of oleochemicals and valuable compounds: Current advances and perspectives	68
Research progress of engineering microbial cell factories for pigment production	68
From lignocellulosic biomass to single cell oil for sustainable biomanufacturing: Current advances and prospects	68
Unlocking the bacterial domain for industrial biotechnology applications using universal parts and tools	68
Improving recombinant protein production in CHO cells using the CRISPR-Cas system	68
Utilization of gene manipulation system for advancing the biotechnological potential of halophiles: A review	68
Expanding sugar alcohol industry: Microbial production of sugar alcohols and associated chemocatalytic derivatives	65
Editorial Board	63
Influence of elevated pressure and pressurized fluids on microenvironment and activity of enzymes	63
Cytoskeleton as a roadmap navigating rhizobia to establish symbiotic root nodulation in legumes	63
Problems and corresponding strategies for converting CO2 into value-added products in Cupriavidus necator H16 cell factories	63
Stress response in Rhodococcus strains	63
C-type lectin-(like) fold – Protein-protein interaction patterns and utilization	63
Strategies for overcoming the limitations of enzymatic carbon dioxide reduction	63
Capturing the third dimension in drug discovery: Spatially-resolved tools for interrogation of complex 3D cell models	62
Detection, production, modification, and application of arylsulfatases	61
Recent advances in enzyme-enhanced immunosensors	61
Recent advances in lipid metabolic engineering of oleaginous yeasts	60
Synthetic biology approaches to improve tolerance of inhibitors in lignocellulosic hydrolysates	60
Genetic engineering of bacteriophages: Key concepts, strategies, and applications	60
Engineering membrane architecture for biotechnological applications	59
Recent advances in engineering synthetic biomolecular condensates	58
Enzyme co-immobilization: Always the biocatalyst designers' choice…or not?	58
Is it possible to shape the microalgal biomass composition with operational parameters for target compound accumulation?	58
Corrigendum to “Recent advances in enzyme-enhanced immunosensors” [Biotechnology Advances 53 (2021) 107867].	57

Editorial Board	57
Biological conversion of carbon monoxide and hydrogen by anaerobic culture: Prospect of anaerobic digestion and thermochemical processes combination	57
Effect of nanomaterials on microbial metabolism and their applications in fermentative hydrogen production: A review	56
A reflection on the improvement of Chinese hamster ovary cell-based bioprocesses through advances in proteomic techniques	56
Synthetic biology of Fusarium for the sustainable production of valuable bioproducts	56
Ex vivo T cell differentiation in adoptive immunotherapy manufacturing: Critical process parameters and analytical technologies	56
Principles of antibodies with ultralong complementarity-determining regions and picobodies	55
One, two, many: Strategies to alter the number of carbohydrate binding sites of lectins	55
Conversion of inulin-rich raw plant biomass to 2,5-furandicarboxylic acid (FDCA): Progress and challenge towards biorenewable plastics	54
A guide to the use of bioassays in exploration of natural resources	54
Advances in multi-enzyme co-localization strategies for the construction of microbial cell factory	54
Production of polyunsaturated fatty acids by Schizochytrium (Aurantiochytrium) spp.	53
Maximizing the potential of nitrilase: Unveiling their diversity, catalytic proficiency, and versatile applications	53
Functionalized chitosan for cancer nano drug delivery	53
Biotechnological applications of type 1 secretion systems	53
Optimizing microbial networks through metabolic bypasses	52
Interpretable and explainable predictive machine learning models for data-driven protein engineering	52
Abiotic stress as a dynamic strategy for enhancing high value phytochemicals in microalgae: Critical insights, challenges and future prospects	52
Photocatalyst-enzyme hybrid systems for light-driven biotransformation	52
The technology, opportunities, and challenges of Synthetic Biological Intelligence	51
Bacterial cellulose and its potential for biomedical applications	51
Biosynthesis of monoterpenoid and sesquiterpenoid as natural flavors and fragrances	50
Advance computational tools for multiomics data learning	50
Heterologous production of cellulose- and starch-degrading hydrolases to expand Saccharomyces cerevisiae substrate utilization: Lessons learnt	50
Improving the performance of machine learning models for biotechnology: The quest for deus ex machina	49
Portable biosensors for rapid on-site determination of cannabinoids in cannabis, a review	49
The Gompertz model and its applications in microbial growth and bioproduction kinetics: Past, present and future	48
Data mining of Saccharomyces cerevisiae mutants engineered for increased tolerance towards inhibitors in lignocellulosic hydrolysates	48
Transcriptomic studies of solventogenic clostridia, Clostridium acetobutylicum and Clostridium beijerinckii	48
Metabolic and biotransformation effects on dietary glucosinolates, their bioavailability, catabolism and biological effects in different organisms	48
Rumen microbes, enzymes, metabolisms, and application in lignocellulosic waste conversion - A comprehensive review	48
Bacterioferritin nanocage: Structure, biological function, catalytic mechanism, self-assembly and potential applications	48
Recent advances in tuning the expression and regulation of genes for constructing microbial cell factories	48
A comprehensive and critical review on key elements to implement enzymatic PET depolymerization for recycling purposes	48
Biofilm control by interfering with c-di-GMP metabolism and signaling	48
Biotechnological advances in the production of unusual fatty acids in transgenic plants and recombinant microorganisms	48
Trait drift in microalgae and applications for strain improvement	48
Recent advances in the identification of biosynthetic genes and gene clusters of the polyketide-derived pathways for anthraquinone biosynthesis and biotechnological applications	47
Design and application of microfluidics in aptamer SELEX and Aptasensors	47
Current status, and the developments of hosts and expression systems for the production of recombinant human cytokines	46
Biopreservation of beer: Potential and constraints	46
New trends in the biocatalytic production of nucleosidic active pharmaceutical ingredients using 2′-deoxyribosyltransferases	46
Protein representations: Encoding biological information for machine learning in biocatalysis	45
Optogenetic technologies in translational cancer research	45
Recent advances and discoveries of microbial-based glycolipids: Prospective alternative for remediation activities	45
From fungal secretomes to enzymes cocktails: The path forward to bioeconomy	44
Plant glycoengineering for designing next-generation vaccines and therapeutic proteins	44
Plants arabinogalactans: From structures to physico-chemical and biological properties	44
Microbial melanin: Recent advances in biosynthesis, extraction, characterization, and applications	43
The isolation of lignin with native-like structure	43
Microbial gas fermentation technology for sustainable food protein production	42
Strategies for the biological production of ectoine by using different chassis strains	42
Challenges and progress towards industrial recombinant protein production in yeasts: A review	42
Recent advances in phyto-combined remediation of heavy metal pollution in soil	41
Strategies to overcome the challenges of low or no expression of heterologous proteins in Escherichia coli	41
Advancements in nanomaterial-based aptasensors for the detection of emerging organic pollutants in environmental and biological samples	41
CRISPR-Cas13 technology portfolio and alliance with other genetic tools	40
Whole-organism phenotypic screening methods used in early-phase anthelmintic drug discovery	40
Influence of glycosylation on the immunogenicity and antigenicity of viral immunogens	40
Recent advances in microbial production of diamines, aminocarboxylic acids, and diacids as potential platform chemicals and bio-based polyamides monomers	40
Engineered transcription factor-binding diversed functional nucleic acid-based synthetic biosensor	40
Controlled processivity in glycosyltransferases: A way to expand the enzymatic toolbox	40
Ferritin nanocage based delivery vehicles: From single-, co- to compartmentalized- encapsulation of bioactive or nutraceutical compounds	40
Turning waste into treasure: A new direction for low-cost production of lipid chemicals from Thraustochytrids	40
Applications of bio-capacitance to cell culture manufacturing	40
Genetically encoded biosensors for microbial synthetic biology: From conceptual frameworks to practical applications	40
Cupriavidus necator as a platform for polyhydroxyalkanoate production: An overview of strains, metabolism, and modeling approaches	39
Fine-tuning gene expression for improved biosynthesis of natural products: From transcriptional to post-translational regulation	39
Bioinformatics assisted construction of the link between biosynthetic gene clusters and secondary metabolites in fungi	38
In vitro models of gut digestion across childhood: current developments, challenges and future trends	37
Fluorescence coupling strategies in fluorescence-activated droplet sorting (FADS) for ultrahigh-throughput screening of enzymes, metabolites, and antibodies	37
HEK-omics: The promise of omics to optimize HEK293 for recombinant adeno-associated virus (rAAV) gene therapy manufacturing	37
Recent advances in microbial production of high-value compounds in the tetrapyrrole biosynthesis pathway	37
The bioproduction of astaxanthin: A comprehensive review on the microbial synthesis and downstream extraction	37
Refactoring transcription factors for metabolic engineering	37
Exploitation of lignocellulosic-based biomass biorefinery: A critical review of renewable bioresource, sustainability and economic views	36
Computational advances in biosynthetic gene cluster discovery and prediction	36
Understanding and controlling the molecular mechanisms of protein aggregation in mAb therapeutics	36
From natural to synthetic: Promoter engineering in yeast expression systems	36
Advances in production and application technologies of minicells: A review	36

Editorial Board	35
Designer bacterial cell factories for improved production of commercially valuable non-ribosomal peptides	35
Editorial Board	35
Selection of aptamers targeting small molecules by capillary electrophoresis: Advances, challenges, and prospects	35
Prospects on bio-based 2,3-butanediol and acetoin production: Recent progress and advances	35
Using oils and fats to replace sugars as feedstocks for biomanufacturing: Challenges and opportunities for the yeast Yarrowia lipolytica	35
Cellular variability as a driver for bioprocess innovation and optimization	34
Utilizing stimuli-responsive nanoparticles to deliver and enhance the anti-tumor effects of bilirubin	34
Humans and robots: Friends of the future? A bird's eye view of biomanufacturing industry 5.0	34
Grafting of proteins onto polymeric surfaces: A synthesis and characterization challenge	34
Strategies to manipulate the performance of aptamers in SELEX, post-SELEX and microenvironment	33
Implications of glycosylation for the development of selected cytokines and their derivatives for medical use	33
The production, recovery, and valorization of polyhydroxybutyrate (PHB) based on circular bioeconomy	33
Microbial extracellular electron transfer and strategies for engineering electroactive microorganisms	33
Heparin and heparin-like modifications in hemodialysis membranes: Current innovations and future directions	32
Current status and future prospects in cannabinoid production through in vitro culture and synthetic biology	32
Host cell protein-mediated adjuvanticity and immunogenicity risks of biotherapeutics	32
Progress in cyclodextrins as important molecules regulating catalytic processes of glycoside hydrolases	32
N-oxygenation of amino compounds: Early stages in its application to the biocatalyzed preparation of bioactive compounds	32
Methods of in vitro study of galectin-glycomaterial interaction	32
The Yin and Yang of exosome isolation methods: conventional practice, microfluidics, and commercial kits	32
Design and biocatalytic applications of genetically fused multifunctional enzymes	31
Review of advances in the development of laccases for the valorization of lignin to enable the production of lignocellulosic biofuels and bioproducts	31
Advances in the synthesis of three typical tetraterpenoids including β-carotene, lycopene and astaxanthin	31
Molecular biology: Fantastic toolkits to improve knowledge and application of acetic acid bacteria	31
Deciphering mechanisms of production of natural compounds using inducer-producer microbial consortia	31
Optimization strategies for CO2 biological fixation	31
Yeast surface display technology: Mechanisms, applications, and perspectives	30
Lipopeptides development in cosmetics and pharmaceutical applications: A comprehensive review	30
Biomass directional pyrolysis based on element economy to produce high-quality fuels, chemicals, carbon materials – A review	30
Editorial Board	30
Biopolymers and their derivatives: Key components of advanced biomedical technologies	30
Has the time finally come for green oleochemicals and biodiesel production using large-scale enzyme technologies? Current status and new developments	30
Identification, characterization, and engineering of glycosylation in thrombolytics	30
Chimeric enzymes in the pulp and paper making industry: Current developments	30
Bacterial membrane vesicle functions, laboratory methods, and applications	30
Strategies for improving antimicrobial peptide production	30
Relieving metabolic burden to improve robustness and bioproduction by industrial microorganisms	30
Editorial Board	30
Molecular imprinting technology for biomedical applications	30
Nucleic acid-responsive smart systems for controlled cargo delivery	29
Mycolicibacterium cell factory for the production of steroid-based drug intermediates	29
Editorial Board	29
Antimicrobial peptides: Sustainable application informed by evolutionary constraints	29
Recent advances in synthetic biology toolkits and metabolic engineering of Ralstonia eutropha H16 for production of value-added chemicals	28
Engineering status of protein for improving microbial cell factories	28
Insights on the emerging biotechnology of histidine-rich peptides	28
Production and characterization of starch-lignin based materials: A review	28
Process simulation and evaluation of scaled-up biocatalytic systems: Advances, challenges, and future prospects	28
Cell-culture process optimization via model-based predictions of metabolism and protein glycosylation	28
Strategies for Automated Enzymatic Glycan Synthesis (AEGS)	28
Biophotovoltaics: Recent advances and perspectives	28
Recent advances in fed-batch microscale bioreactor design	27
Next generation probiotics: Engineering live biotherapeutics	27
Advanced strategies for metabolic engineering of Bacillus to produce extracellular polymeric substances	27
Biodegradation of polyethylene and polystyrene: From microbial deterioration to enzyme discovery	26
Nanoantibiotics to fight multidrug resistant infections by Gram-positive bacteria: hope or reality?	26
4D printing: Perspectives for the production of sustainable plastics for agriculture	26
Editorial Board	26
The signaling pathways involved in metabolic regulation and stress responses of the yeast-like fungi Aureobasidium spp.	26
Role of distal sites in enzyme engineering	26
Expanding the horizon of transient CAR T therapeutics using virus-free technology	26
Engineering conditional protein-protein interactions for dynamic cellular control	26
The new frontier in CHO cell line development: From random to targeted transgene integration technologies	25
Extremophilic lipases for industrial applications: A general review	25
Fungal ergot alkaloids: Metabolic pathways, biological functions, and advances in synthetic reprogramming	25
Insights to improve the plant nutrient transport by CRISPR/Cas system	25
Editorial Board	25
Trends in the development of innovative nanobiocatalysts and their application in biocatalytic transformations	25
The vast repertoire of carbohydrate oxidases: An overview	25
Mapping the architecture of animal toxin systems by mass spectrometry imaging	25
Editorial Board	25
Biosynthesis of alkanes/alkenes from fatty acids or derivatives (triacylglycerols or fatty aldehydes)	25
The role of orphan crops in the transition to nutritional quality-oriented crop improvement	25
Current innovations in mRNA vaccines for targeting multidrug-resistant ESKAPE pathogens	25
The evolving art of creating genetic diversity: From directed evolution to synthetic biology	25
Towards efficient enzymatic saccharification of pretreated lignocellulose: Enzyme inhibition by lignin-derived phenolics and recent trends in mitigation strategies	24
Industrial bioelectrochemistry for waste valorization: State of the art and challenges	24
Designing artificial pathways for improving chemical production	24
Trends in digital image processing of isolated microalgae by incorporating classification algorithm	24
Quantifying the impact of cell culture media on CHO cell growth and protein production	24
In-depth analysis of biocatalysts by microfluidics: An emerging source of data for machine learning	24
Alternative biological sources for extracellular vesicles production and purification strategies for process scale-up	23
Agrobacterium strains and strain improvement: Present and outlook	23
Challenges in developing cell culture media using machine learning	23
Biocatalytic production of biolubricants: Strategies, problems and future trends	23
Editorial Board	23
Recent advances in the application of CRISPR/Cas-based gene editing technology in Filamentous Fungi	23
Decoding cellular mechanism of recombinant adeno-associated virus (rAAV) and engineering host-cell factories toward intensified viral vector manufacturing	23
Editorial Board	23
Microbial transformations by sulfur bacteria can recover value from phosphogypsum: A global problem and a possible solution	23
Towards a seamless product and process development workflow for recombinant proteins produced by plant molecular farming	22
Advances in synthetic biology toolboxes paving the way for mechanistic understanding and strain engineering of gut commensal Bacteroides spp. and Clostridium spp.	22
Building a circular economy around poly(D/L-γ-glutamic acid)- a smart microbial biopolymer	22
Dehydrogenases of acetic acid bacteria	22