Biotechnology Advances

Article	Citations
Editorial Board	469
Targeted analysis of metagenomes: divide and conquer	249
Collagen processing with mesoscale aggregates as templates and building blocks	247
Anaerobic digestion integrated with microbial electrolysis cell to enhance biogas production and upgrading in situ	187
New perspectives into Gluconobacter-catalysed biotransformations	184
Microbial cell surface engineering for high-level synthesis of bio-products	166
Computer-aided understanding and engineering of enzymatic selectivity	162
Biotechnological production and emerging applications of betalains: A review	159
Evolving perspectives on lutein production from microalgae - A focus on productivity and heterotrophic culture	157
Advancements of astaxanthin production in Haematococcus pluvialis: Update insight and way forward	150
Halocins, natural antimicrobials of Archaea: Exotic or special or both?	147
Development of isopentenyl phosphate kinases and their application in terpenoid biosynthesis	143
Use of additives to improve collective biogas plant performances: A comprehensive review	142
Methanol metabolism in synthetic methylotrophic microorganisms	134
Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies	129
Recent advances in human respiratory epithelium models for drug discovery	128
Chiral helical scaffolds: Unlocking their potential in biomolecular interactions and biomedical applications	124
Think outside the box: 3D bioprinting concepts for biotechnological applications – recent developments and future perspectives	123
Biosensors in microalgae: A roadmap for new opportunities in synthetic biology and biotechnology	118
Glycosylation in extracellular vesicles: Isolation, characterization, composition, analysis and clinical applications	117
Opportunities for visual techniques to determine characteristics and limitations of electro-active biofilms	110
Accessing hidden microbial biosynthetic potential from underexplored sources for novel drug discovery	110
Potential for microbial plastic degradation via assimilation of non-carbon moieties in additives	106
Pickering emulsion biocatalysis: Bridging interfacial design with enzymatic reactions	105
Phosphorylated polysaccharides: Applications, natural abundance, and new-to-nature structures generated by chemical and enzymatic functionalisation	105

Recent advances and perspectives on production of value-added organic acids through metabolic engineering	104
Alpha-ketoacid decarboxylases: Diversity, structures, reaction mechanisms, and applications for biomanufacturing of platform chemicals and fuels	103
The path to biotechnological singularity: Current breakthroughs and outlook	100
Editorial Board	97
Emerging research insights and future perspectives on the advancement of eccDNA: A comprehensive review	97
Editorial Board	96
Biological valorization of lignin to flavonoids	93
Enhancing single-cell bioconversion efficiency by harnessing nanosecond pulsed electric field processing	93
Unlocking the secret of lignin-enzyme interactions: Recent advances in developing state-of-the-art analytical techniques	92
Advances in gene editing-led route for hybrid breeding in crops	92
Charting the landscape of RNA polymerases to unleash their potential in strain improvement	90
Biotechnological applications of purine and pyrimidine deaminases	90
Polyethylene biodegradation: A multifaceted approach	87
Elucidation and biosynthesis of tetrahydroisoquinoline alkaloids: Recent advances and prospects	87
Growth-coupled high throughput selection for directed enzyme evolution	87
Methanotrophs as a reservoir for bioactive secondary metabolites: Pitfalls, insights and promises	86
Genome-scale metabolic network models for industrial microorganisms metabolic engineering: Current advances and future prospects	86
Polyhydroxyalkanoates synthesis by halophiles and thermophiles: towards sustainable production of microbial bioplastics	83
Ex-situ biomethanation for CO2 valorization: State of the art, recent advances, challenges, and future prospective	83
Haloferax and the Haloferacaceae: Potential role in bioindustry	81
Protease engineering: Approaches, tools, and emerging trends	81
Recent advances in machine learning applications in metabolic engineering	80
Biocatalytic oxyfunctionalization of unsaturated fatty acids to oxygenated chemicals via hydroxy fatty acids	80
Electrode functional microorganisms in bioelectrochemical systems and its regulation: A review	79
Enzymatic de novo oligonucleotide synthesis: Emerging techniques and advancements	78
Advances in bacterial glycoprotein engineering: A critical review of current technologies, emerging challenges, and future directions	78
Advances in in vitro cultivation techniques for comprehensive analysis of human gut microbiome	77
Strategies for overcoming the limitations of enzymatic carbon dioxide reduction	76
Recent advances in research for potential utilization of unexplored lichen metabolites	75
Guidelines toward ecologically-informed bioprospecting for microbial plastic degradation	75
Emerging advances in glycoengineering of carbohydrates/glycans and their industrial applications	75
Unlocking the bacterial domain for industrial biotechnology applications using universal parts and tools	75
Influence of elevated pressure and pressurized fluids on microenvironment and activity of enzymes	74
Fusarium as a promising fungal genus with potential application in bioremediation for pollutants mitigation: A review	74
Stress response in Rhodococcus strains	72
Bioinspired nanoplatforms for human-machine interfaces: Recent progress in materials and device applications	71
From lignocellulosic biomass to single cell oil for sustainable biomanufacturing: Current advances and prospects	70
Engineering oleaginous red yeasts as versatile chassis for the production of oleochemicals and valuable compounds: Current advances and perspectives	69
Improving recombinant protein production in CHO cells using the CRISPR-Cas system	68
Resource allocation in mammalian systems	68
Research progress of engineering microbial cell factories for pigment production	68
Problems and corresponding strategies for converting CO2 into value-added products in Cupriavidus necator H16 cell factories	67
Utilization of gene manipulation system for advancing the biotechnological potential of halophiles: A review	67
Rutinosidase and other diglycosidases: Rising stars in biotechnology	67
Expanding sugar alcohol industry: Microbial production of sugar alcohols and associated chemocatalytic derivatives	66
C-type lectin-(like) fold – Protein-protein interaction patterns and utilization	65
Capturing the third dimension in drug discovery: Spatially-resolved tools for interrogation of complex 3D cell models	65
Editorial Board	65
Cytoskeleton as a roadmap navigating rhizobia to establish symbiotic root nodulation in legumes	64
Recent advances in lipid metabolic engineering of oleaginous yeasts	64

Harnessing the biomolecular mechanisms of marine biomineralisation for carbon sequestration	64
Machine learning in predictive biocatalysis: A comparative review of methods and applications	63
Recent advances in enzyme-enhanced immunosensors	63
Microalgal bioengineering for futuristic applications in synthetic and space biology	63
Specialized pro-resolving mediators: Biosynthetic pathways, biocatalytic synthesis, and applications	63
Tracking inflammation status for improving patient prognosis: A review of current methods, unmet clinical needs and opportunities	63
Is it possible to shape the microalgal biomass composition with operational parameters for target compound accumulation?	62
Engineering membrane architecture for biotechnological applications	61
Genetic engineering of bacteriophages: Key concepts, strategies, and applications	61
Detection, production, modification, and application of arylsulfatases	61
Synthetic biology approaches to improve tolerance of inhibitors in lignocellulosic hydrolysates	59
Enzyme co-immobilization: Always the biocatalyst designers' choice…or not?	59
Maximizing the potential of nitrilase: Unveiling their diversity, catalytic proficiency, and versatile applications	58
Recent advances in engineering synthetic biomolecular condensates	58
Recent advances in chemoenzymatic synthesis of human ABO blood group antigens	58
Corrigendum to “Recent advances in enzyme-enhanced immunosensors” [Biotechnology Advances 53 (2021) 107867].	58
Biological conversion of carbon monoxide and hydrogen by anaerobic culture: Prospect of anaerobic digestion and thermochemical processes combination	58
Synthetic biology of Fusarium for the sustainable production of valuable bioproducts	58
Heterologous production of cellulose- and starch-degrading hydrolases to expand Saccharomyces cerevisiae substrate utilization: Lessons learnt	57
The technology, opportunities, and challenges of Synthetic Biological Intelligence	57
Conversion of inulin-rich raw plant biomass to 2,5-furandicarboxylic acid (FDCA): Progress and challenge towards biorenewable plastics	57
A reflection on the improvement of Chinese hamster ovary cell-based bioprocesses through advances in proteomic techniques	57
Production of polyunsaturated fatty acids by Schizochytrium (Aurantiochytrium) spp.	57
A guide to the use of bioassays in exploration of natural resources	56
Abiotic stress as a dynamic strategy for enhancing high value phytochemicals in microalgae: Critical insights, challenges and future prospects	56
Photocatalyst-enzyme hybrid systems for light-driven biotransformation	56
Brews, fuels, and opioids: Expanding the yeast Ehrlich pathway for chemical and pharmaceutical manufacturing	55
Bacterial cellulose and its potential for biomedical applications	55
Enzyme miniaturization: Revolutionizing future biocatalysts	55
Principles of antibodies with ultralong complementarity-determining regions and picobodies	54
Editorial Board	54
Bio-upcycling PET waste: Advances in enzymatic hydrolysis and biosynthesis of value-added products	54
Ex vivo T cell differentiation in adoptive immunotherapy manufacturing: Critical process parameters and analytical technologies	53
Algae-driven bioelectrochemical systems: Recent advances, applications, and prospects	53
Advances in multi-enzyme co-localization strategies for the construction of microbial cell factory	52
Interpretable and explainable predictive machine learning models for data-driven protein engineering	52
Effect of nanomaterials on microbial metabolism and their applications in fermentative hydrogen production: A review	52
Advance computational tools for multiomics data learning	52
Biotechnological applications of type 1 secretion systems	52
Optimizing microbial networks through metabolic bypasses	51
Functionalized chitosan for cancer nano drug delivery	51
One, two, many: Strategies to alter the number of carbohydrate binding sites of lectins	51
Biosynthesis of monoterpenoid and sesquiterpenoid as natural flavors and fragrances	50
A comprehensive and critical review on key elements to implement enzymatic PET depolymerization for recycling purposes	49
Biotechnological advances in the production of unusual fatty acids in transgenic plants and recombinant microorganisms	49
Rumen microbes, enzymes, metabolisms, and application in lignocellulosic waste conversion - A comprehensive review	49
The Gompertz model and its applications in microbial growth and bioproduction kinetics: Past, present and future	49
The stressing point: how plants respond to environmental stimuli	49
Data mining of Saccharomyces cerevisiae mutants engineered for increased tolerance towards inhibitors in lignocellulosic hydrolysates	49
Transcriptomic studies of solventogenic clostridia, Clostridium acetobutylicum and Clostridium beijerinckii	49
Portable biosensors for rapid on-site determination of cannabinoids in cannabis, a review	48
Trait drift in microalgae and applications for strain improvement	48
Fungal bioconversion of lignin-derived aromatics: Pathways, enzymes, and biotechnological potential	47
Enzymatic C N bond formation and cleavage reaction: Advances and perspectives	47
Bacterioferritin nanocage: Structure, biological function, catalytic mechanism, self-assembly and potential applications	47
Bioactive metabolites and extracellular vesicles from the marine chlorophyte genus Tetraselmis: Review	47
Current status, and the developments of hosts and expression systems for the production of recombinant human cytokines	46
Improving the performance of machine learning models for biotechnology: The quest for deus ex machina	46
Biofilm control by interfering with c-di-GMP metabolism and signaling	46
Metabolic and biotransformation effects on dietary glucosinolates, their bioavailability, catabolism and biological effects in different organisms	46
Plant glycoengineering for designing next-generation vaccines and therapeutic proteins	45
New trends in the biocatalytic production of nucleosidic active pharmaceutical ingredients using 2′-deoxyribosyltransferases	45
Optogenetic technologies in translational cancer research	45
Plants arabinogalactans: From structures to physico-chemical and biological properties	44
Microbial gas fermentation technology for sustainable food protein production	44
Recent advances in the identification of biosynthetic genes and gene clusters of the polyketide-derived pathways for anthraquinone biosynthesis and biotechnological applications	43
Strategies for the biological production of ectoine by using different chassis strains	43
From fungal secretomes to enzymes cocktails: The path forward to bioeconomy	42
Biopreservation of beer: Potential and constraints	42
Strategies to overcome the challenges of low or no expression of heterologous proteins in Escherichia coli	41
Microbial melanin: Recent advances in biosynthesis, extraction, characterization, and applications	41
The isolation of lignin with native-like structure	41
Design and application of microfluidics in aptamer SELEX and Aptasensors	41
Protein representations: Encoding biological information for machine learning in biocatalysis	41
Advancements in nanomaterial-based aptasensors for the detection of emerging organic pollutants in environmental and biological samples	40
Challenges and progress towards industrial recombinant protein production in yeasts: A review	40
Yeast as C1 cell factory: Transforming methanol and Formate into high-value compounds	40
Recent advances and discoveries of microbial-based glycolipids: Prospective alternative for remediation activities	40
Cupriavidus necator as a platform for polyhydroxyalkanoate production: An overview of strains, metabolism, and modeling approaches	40
Recent advances in phyto-combined remediation of heavy metal pollution in soil	40

Engineered transcription factor-binding diversed functional nucleic acid-based synthetic biosensor	40
Whole-organism phenotypic screening methods used in early-phase anthelmintic drug discovery	39
Genetic engineering of Saccharomyces boulardii: Tools, strategies and advances for enhanced probiotic and therapeutic applications	39
Applications of bio-capacitance to cell culture manufacturing	39
Recent advances in microbial production of diamines, aminocarboxylic acids, and diacids as potential platform chemicals and bio-based polyamides monomers	38
CRISPR-Cas13 technology portfolio and alliance with other genetic tools	38
Influence of glycosylation on the immunogenicity and antigenicity of viral immunogens	38
Turning waste into treasure: A new direction for low-cost production of lipid chemicals from Thraustochytrids	38
Fine-tuning gene expression for improved biosynthesis of natural products: From transcriptional to post-translational regulation	38
Controlled processivity in glycosyltransferases: A way to expand the enzymatic toolbox	38
Multidimensional strategies for efficient heterologous protein expression in Aspergillus niger	37
Genetically encoded biosensors for microbial synthetic biology: From conceptual frameworks to practical applications	37
Ferritin nanocage based delivery vehicles: From single-, co- to compartmentalized- encapsulation of bioactive or nutraceutical compounds	37
Advances in production and application technologies of minicells: A review	37
Designer bacterial cell factories for improved production of commercially valuable non-ribosomal peptides	36
Computational advances in biosynthetic gene cluster discovery and prediction	36
From natural to synthetic: Promoter engineering in yeast expression systems	36
HEK-omics: The promise of omics to optimize HEK293 for recombinant adeno-associated virus (rAAV) gene therapy manufacturing	36
Using oils and fats to replace sugars as feedstocks for biomanufacturing: Challenges and opportunities for the yeast Yarrowia lipolytica	36
Advances of computational protein design: Principles, strategies and applications in nutrition and health	36
Understanding and controlling the molecular mechanisms of protein aggregation in mAb therapeutics	36
Bioinformatics assisted construction of the link between biosynthetic gene clusters and secondary metabolites in fungi	36
Metagenomics and plant-microbe symbioses: Microbial community dynamics, functional roles in carbon sequestration, nitrogen transformation, sulfur and phosphorus mobilization for sustainable soil healt	35
Recent advances in microbial production of high-value compounds in the tetrapyrrole biosynthesis pathway	35
Exploitation of lignocellulosic-based biomass biorefinery: A critical review of renewable bioresource, sustainability and economic views	35
Refactoring transcription factors for metabolic engineering	35
Selection of aptamers targeting small molecules by capillary electrophoresis: Advances, challenges, and prospects	34
The bioproduction of astaxanthin: A comprehensive review on the microbial synthesis and downstream extraction	34
In vitro models of gut digestion across childhood: current developments, challenges and future trends	34
Cellular variability as a driver for bioprocess innovation and optimization	33
Fluorescence coupling strategies in fluorescence-activated droplet sorting (FADS) for ultrahigh-throughput screening of enzymes, metabolites, and antibodies	33
Editorial Board	33
Editorial Board	33
Prospects on bio-based 2,3-butanediol and acetoin production: Recent progress and advances	33
Implications of glycosylation for the development of selected cytokines and their derivatives for medical use	32
Utilizing stimuli-responsive nanoparticles to deliver and enhance the anti-tumor effects of bilirubin	32
Strategies to manipulate the performance of aptamers in SELEX, post-SELEX and microenvironment	32
From formate oxidation to CO₂ reduction: The role of formate dehydrogenase in sustainable carbon utilization	32
The production, recovery, and valorization of polyhydroxybutyrate (PHB) based on circular bioeconomy	32
Revolutionizing agroindustry: Towards the industrial application of antimicrobial peptides against pathogens and pests	32
Grafting of proteins onto polymeric surfaces: A synthesis and characterization challenge	32
Humans and robots: Friends of the future? A bird's eye view of biomanufacturing industry 5.0	32
The Yin and Yang of exosome isolation methods: conventional practice, microfluidics, and commercial kits	32
Myxobacteria: Versatile cell factories of novel commercial enzymes for bio-manufacturing	32
Understanding low-phosphate stress responses in plants: Opportunities for genome editing to improve phosphorous use efficiency (PUE)	32
Microbial extracellular electron transfer and strategies for engineering electroactive microorganisms	32
Host cell protein-mediated adjuvanticity and immunogenicity risks of biotherapeutics	32
Yeast surface display technology: Mechanisms, applications, and perspectives	31
Biomass directional pyrolysis based on element economy to produce high-quality fuels, chemicals, carbon materials – A review	31
Molecular imprinting technology for biomedical applications	31
Current status and future prospects in cannabinoid production through in vitro culture and synthetic biology	31
Design and biocatalytic applications of genetically fused multifunctional enzymes	31
Biopolymers and their derivatives: Key components of advanced biomedical technologies	30
Revolutionizing keratinase science: Biocatalytic advances, sustainable innovation, and industrial perspectives	30
Methods of in vitro study of galectin-glycomaterial interaction	30
Progress in cyclodextrins as important molecules regulating catalytic processes of glycoside hydrolases	30
Molecular biology: Fantastic toolkits to improve knowledge and application of acetic acid bacteria	30
Epigenetic therapy for leukemias: a comprehensive review from biological markers to practice	30
N-oxygenation of amino compounds: Early stages in its application to the biocatalyzed preparation of bioactive compounds	30
Bacterial membrane vesicle functions, laboratory methods, and applications	30
Deciphering mechanisms of production of natural compounds using inducer-producer microbial consortia	30
Advances in the synthesis of three typical tetraterpenoids including β-carotene, lycopene and astaxanthin	30
Heparin and heparin-like modifications in hemodialysis membranes: Current innovations and future directions	29
Lipopeptides development in cosmetics and pharmaceutical applications: A comprehensive review	29
Relieving metabolic burden to improve robustness and bioproduction by industrial microorganisms	29
Review of advances in the development of laccases for the valorization of lignin to enable the production of lignocellulosic biofuels and bioproducts	29
Optimization strategies for CO2 biological fixation	29
Has the time finally come for green oleochemicals and biodiesel production using large-scale enzyme technologies? Current status and new developments	29
Editorial Board	28
Process simulation and evaluation of scaled-up biocatalytic systems: Advances, challenges, and future prospects	28
Nucleic acid-responsive smart systems for controlled cargo delivery	28
Engineering catalytically promiscuous enzymes to serve new functions	28
Strategies for improving antimicrobial peptide production	28
Editorial Board	27
Morphology engineering facilitates constructing efficient cell factories	27
Identification, characterization, and engineering of glycosylation in thrombolytics	27
Antimicrobial peptides: Sustainable application informed by evolutionary constraints	27
Editorial Board	27
Insights on the emerging biotechnology of histidine-rich peptides	27
Production and characterization of starch-lignin based materials: A review	27
Current advancement in AI-integrated drug discovery: Methods and applications	27
Baculovirus dual-phase infection strategy and biotechnological applications: A structural and regulatory review	27
Engineering status of protein for improving microbial cell factories	27
Biodegradation of polyethylene and polystyrene: From microbial deterioration to enzyme discovery	26
A review on the expanding biotechnological frontier of Pedobacter	26
Cell-culture process optimization via model-based predictions of metabolism and protein glycosylation	26
Role of distal sites in enzyme engineering	26
Nanoantibiotics to fight multidrug resistant infections by Gram-positive bacteria: hope or reality?	26
Mycolicibacterium cell factory for the production of steroid-based drug intermediates	26
Biophotovoltaics: Recent advances and perspectives	26
Advanced strategies for metabolic engineering of Bacillus to produce extracellular polymeric substances	26
Next generation probiotics: Engineering live biotherapeutics	26
Recent advances in fed-batch microscale bioreactor design	26
Strategies for Automated Enzymatic Glycan Synthesis (AEGS)	26
Chimeric enzymes in the pulp and paper making industry: Current developments	26