Biotechnology Advances

Article	Citations
Editorial Board	455
Advancements of astaxanthin production in Haematococcus pluvialis: Update insight and way forward	230
Development of isopentenyl phosphate kinases and their application in terpenoid biosynthesis	219
Halocins, natural antimicrobials of Archaea: Exotic or special or both?	182
Biotechnological production and emerging applications of betalains: A review	174
Evolving perspectives on lutein production from microalgae - A focus on productivity and heterotrophic culture	158
Use of additives to improve collective biogas plant performances: A comprehensive review	154
Computer-aided understanding and engineering of enzymatic selectivity	147
Recent advances in human respiratory epithelium models for drug discovery	141
New perspectives into Gluconobacter-catalysed biotransformations	139
Microbial cell surface engineering for high-level synthesis of bio-products	137
Anaerobic digestion integrated with microbial electrolysis cell to enhance biogas production and upgrading in situ	131
Collagen processing with mesoscale aggregates as templates and building blocks	130
Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies	130
Phosphorylated polysaccharides: Applications, natural abundance, and new-to-nature structures generated by chemical and enzymatic functionalisation	123
Pickering emulsion biocatalysis: Bridging interfacial design with enzymatic reactions	121
Accessing hidden microbial biosynthetic potential from underexplored sources for novel drug discovery	119
Chiral helical scaffolds: Unlocking their potential in biomolecular interactions and biomedical applications	112
Glycosylation in extracellular vesicles: Isolation, characterization, composition, analysis and clinical applications	111
Biosensors in microalgae: A roadmap for new opportunities in synthetic biology and biotechnology	107
Think outside the box: 3D bioprinting concepts for biotechnological applications – recent developments and future perspectives	104
Recent advances and perspectives on production of value-added organic acids through metabolic engineering	103
Opportunities for visual techniques to determine characteristics and limitations of electro-active biofilms	103
Polyethylene biodegradation: A multifaceted approach	102
Alpha-ketoacid decarboxylases: Diversity, structures, reaction mechanisms, and applications for biomanufacturing of platform chemicals and fuels	98

Protease engineering: Approaches, tools, and emerging trends	98
Editorial Board	97
Elucidation and biosynthesis of tetrahydroisoquinoline alkaloids: Recent advances and prospects	96
Biotechnological applications of purine and pyrimidine deaminases	91
Enhancing single-cell bioconversion efficiency by harnessing nanosecond pulsed electric field processing	91
Methanotrophs as a reservoir for bioactive secondary metabolites: Pitfalls, insights and promises	90
Advances in gene editing-led route for hybrid breeding in crops	90
Charting the landscape of RNA polymerases to unleash their potential in strain improvement	89
Growth-coupled high throughput selection for directed enzyme evolution	86
Biocatalytic oxyfunctionalization of unsaturated fatty acids to oxygenated chemicals via hydroxy fatty acids	86
Enzymatic de novo oligonucleotide synthesis: Emerging techniques and advancements	85
Unlocking the secret of lignin-enzyme interactions: Recent advances in developing state-of-the-art analytical techniques	84
Microbial starch debranching enzymes: Developments and applications	84
Genome-scale metabolic network models for industrial microorganisms metabolic engineering: Current advances and future prospects	82
From lignocellulose to plastics: Knowledge transfer on the degradation approaches by fungi	80
Recent advances in machine learning applications in metabolic engineering	78
Polyhydroxyalkanoates synthesis by halophiles and thermophiles: towards sustainable production of microbial bioplastics	78
Electrode functional microorganisms in bioelectrochemical systems and its regulation: A review	77
Biological valorization of lignin to flavonoids	77
Ex-situ biomethanation for CO2 valorization: State of the art, recent advances, challenges, and future prospective	77
Emerging advances in glycoengineering of carbohydrates/glycans and their industrial applications	75
Advances in bacterial glycoprotein engineering: A critical review of current technologies, emerging challenges, and future directions	75
Rutinosidase and other diglycosidases: Rising stars in biotechnology	74
Bioinspired nanoplatforms for human-machine interfaces: Recent progress in materials and device applications	73
Improving recombinant protein production in CHO cells using the CRISPR-Cas system	73
Advances in in vitro cultivation techniques for comprehensive analysis of human gut microbiome	72
Recent advances in research for potential utilization of unexplored lichen metabolites	72
Guidelines toward ecologically-informed bioprospecting for microbial plastic degradation	72
Utilization of gene manipulation system for advancing the biotechnological potential of halophiles: A review	71
Expanding sugar alcohol industry: Microbial production of sugar alcohols and associated chemocatalytic derivatives	70
Strategies for overcoming the limitations of enzymatic carbon dioxide reduction	70
Influence of elevated pressure and pressurized fluids on microenvironment and activity of enzymes	69
From lignocellulosic biomass to single cell oil for sustainable biomanufacturing: Current advances and prospects	68
Fusarium as a promising fungal genus with potential application in bioremediation for pollutants mitigation: A review	67
Unlocking the bacterial domain for industrial biotechnology applications using universal parts and tools	65
Research progress of engineering microbial cell factories for pigment production	64
Problems and corresponding strategies for converting CO2 into value-added products in Cupriavidus necator H16 cell factories	64
Stress response in Rhodococcus strains	64
Engineering oleaginous red yeasts as versatile chassis for the production of oleochemicals and valuable compounds: Current advances and perspectives	64
Editorial Board	63
Resource allocation in mammalian systems	63
Cytoskeleton as a roadmap navigating rhizobia to establish symbiotic root nodulation in legumes	62
Capturing the third dimension in drug discovery: Spatially-resolved tools for interrogation of complex 3D cell models	62
Recent advances in enzyme-enhanced immunosensors	61
Synthetic biology approaches to improve tolerance of inhibitors in lignocellulosic hydrolysates	61
Biological conversion of carbon monoxide and hydrogen by anaerobic culture: Prospect of anaerobic digestion and thermochemical processes combination	61
Recent advances in lipid metabolic engineering of oleaginous yeasts	60
Genetic engineering of bacteriophages: Key concepts, strategies, and applications	60
Recent advances in engineering synthetic biomolecular condensates	60
Is it possible to shape the microalgal biomass composition with operational parameters for target compound accumulation?	60

Detection, production, modification, and application of arylsulfatases	60
C-type lectin-(like) fold – Protein-protein interaction patterns and utilization	59
Enzyme co-immobilization: Always the biocatalyst designers' choice…or not?	59
Tracking inflammation status for improving patient prognosis: A review of current methods, unmet clinical needs and opportunities	59
Corrigendum to “Recent advances in enzyme-enhanced immunosensors” [Biotechnology Advances 53 (2021) 107867].	58
Engineering membrane architecture for biotechnological applications	58
Editorial Board	57
Advances in molecular tools for parasitic nematodes of animals – From genetic markers to metabarcoding and genomics	57
Heterologous production of cellulose- and starch-degrading hydrolases to expand Saccharomyces cerevisiae substrate utilization: Lessons learnt	57
The technology, opportunities, and challenges of Synthetic Biological Intelligence	56
Ex vivo T cell differentiation in adoptive immunotherapy manufacturing: Critical process parameters and analytical technologies	55
Recent advances in chemoenzymatic synthesis of human ABO blood group antigens	55
Enzyme miniaturization: Revolutionizing future biocatalysts	55
Principles of antibodies with ultralong complementarity-determining regions and picobodies	55
Synthetic biology of Fusarium for the sustainable production of valuable bioproducts	55
Optimizing microbial networks through metabolic bypasses	54
Conversion of inulin-rich raw plant biomass to 2,5-furandicarboxylic acid (FDCA): Progress and challenge towards biorenewable plastics	54
Biotechnological applications of type 1 secretion systems	54
Maximizing the potential of nitrilase: Unveiling their diversity, catalytic proficiency, and versatile applications	54
Abiotic stress as a dynamic strategy for enhancing high value phytochemicals in microalgae: Critical insights, challenges and future prospects	53
Photocatalyst-enzyme hybrid systems for light-driven biotransformation	53
Interpretable and explainable predictive machine learning models for data-driven protein engineering	53
A guide to the use of bioassays in exploration of natural resources	52
Effect of nanomaterials on microbial metabolism and their applications in fermentative hydrogen production: A review	52
Advance computational tools for multiomics data learning	52
A reflection on the improvement of Chinese hamster ovary cell-based bioprocesses through advances in proteomic techniques	52
Biosynthesis of monoterpenoid and sesquiterpenoid as natural flavors and fragrances	51
One, two, many: Strategies to alter the number of carbohydrate binding sites of lectins	51
Bacterial cellulose and its potential for biomedical applications	51
Functionalized chitosan for cancer nano drug delivery	51
Metabolic and biotransformation effects on dietary glucosinolates, their bioavailability, catabolism and biological effects in different organisms	50
Production of polyunsaturated fatty acids by Schizochytrium (Aurantiochytrium) spp.	50
Bacterioferritin nanocage: Structure, biological function, catalytic mechanism, self-assembly and potential applications	50
Advances in multi-enzyme co-localization strategies for the construction of microbial cell factory	50
Trait drift in microalgae and applications for strain improvement	49
Portable biosensors for rapid on-site determination of cannabinoids in cannabis, a review	49
Rumen microbes, enzymes, metabolisms, and application in lignocellulosic waste conversion - A comprehensive review	49
Improving the performance of machine learning models for biotechnology: The quest for deus ex machina	49
A comprehensive and critical review on key elements to implement enzymatic PET depolymerization for recycling purposes	48
Biofilm control by interfering with c-di-GMP metabolism and signaling	48
Transcriptomic studies of solventogenic clostridia, Clostridium acetobutylicum and Clostridium beijerinckii	48
Data mining of Saccharomyces cerevisiae mutants engineered for increased tolerance towards inhibitors in lignocellulosic hydrolysates	48
The Gompertz model and its applications in microbial growth and bioproduction kinetics: Past, present and future	47
Biotechnological advances in the production of unusual fatty acids in transgenic plants and recombinant microorganisms	47
Recent advances in the identification of biosynthetic genes and gene clusters of the polyketide-derived pathways for anthraquinone biosynthesis and biotechnological applications	46
New trends in the biocatalytic production of nucleosidic active pharmaceutical ingredients using 2′-deoxyribosyltransferases	46
Recent advances in tuning the expression and regulation of genes for constructing microbial cell factories	46
Design and application of microfluidics in aptamer SELEX and Aptasensors	46
Strategies to overcome the challenges of low or no expression of heterologous proteins in Escherichia coli	45
Strategies for the biological production of ectoine by using different chassis strains	45
Plant glycoengineering for designing next-generation vaccines and therapeutic proteins	45
Advancements in nanomaterial-based aptasensors for the detection of emerging organic pollutants in environmental and biological samples	44
Biopreservation of beer: Potential and constraints	44
The isolation of lignin with native-like structure	44
Microbial melanin: Recent advances in biosynthesis, extraction, characterization, and applications	44
Microbial gas fermentation technology for sustainable food protein production	44
Recent advances and discoveries of microbial-based glycolipids: Prospective alternative for remediation activities	44
From fungal secretomes to enzymes cocktails: The path forward to bioeconomy	43
Optogenetic technologies in translational cancer research	42
Current status, and the developments of hosts and expression systems for the production of recombinant human cytokines	42
Recent advances in phyto-combined remediation of heavy metal pollution in soil	42
Protein representations: Encoding biological information for machine learning in biocatalysis	41
Challenges and progress towards industrial recombinant protein production in yeasts: A review	41
Ferritin nanocage based delivery vehicles: From single-, co- to compartmentalized- encapsulation of bioactive or nutraceutical compounds	40
Influence of glycosylation on the immunogenicity and antigenicity of viral immunogens	40
Engineered transcription factor-binding diversed functional nucleic acid-based synthetic biosensor	40
Plants arabinogalactans: From structures to physico-chemical and biological properties	40
CRISPR-Cas13 technology portfolio and alliance with other genetic tools	39
Genetically encoded biosensors for microbial synthetic biology: From conceptual frameworks to practical applications	39
Applications of bio-capacitance to cell culture manufacturing	39
Fine-tuning gene expression for improved biosynthesis of natural products: From transcriptional to post-translational regulation	38
Recent advances in microbial production of diamines, aminocarboxylic acids, and diacids as potential platform chemicals and bio-based polyamides monomers	38
Cupriavidus necator as a platform for polyhydroxyalkanoate production: An overview of strains, metabolism, and modeling approaches	38
Controlled processivity in glycosyltransferases: A way to expand the enzymatic toolbox	38
Whole-organism phenotypic screening methods used in early-phase anthelmintic drug discovery	38
Designer bacterial cell factories for improved production of commercially valuable non-ribosomal peptides	37
Turning waste into treasure: A new direction for low-cost production of lipid chemicals from Thraustochytrids	37
HEK-omics: The promise of omics to optimize HEK293 for recombinant adeno-associated virus (rAAV) gene therapy manufacturing	37
Bioinformatics assisted construction of the link between biosynthetic gene clusters and secondary metabolites in fungi	37
Selection of aptamers targeting small molecules by capillary electrophoresis: Advances, challenges, and prospects	37

Computational advances in biosynthetic gene cluster discovery and prediction	36
Understanding and controlling the molecular mechanisms of protein aggregation in mAb therapeutics	36
Recent advances in microbial production of high-value compounds in the tetrapyrrole biosynthesis pathway	36
The bioproduction of astaxanthin: A comprehensive review on the microbial synthesis and downstream extraction	36
Metagenomics and plant-microbe symbioses: Microbial community dynamics, functional roles in carbon sequestration, nitrogen transformation, sulfur and phosphorus mobilization for sustainable soil healt	36
Fluorescence coupling strategies in fluorescence-activated droplet sorting (FADS) for ultrahigh-throughput screening of enzymes, metabolites, and antibodies	36
In vitro models of gut digestion across childhood: current developments, challenges and future trends	36
From natural to synthetic: Promoter engineering in yeast expression systems	35
Using oils and fats to replace sugars as feedstocks for biomanufacturing: Challenges and opportunities for the yeast Yarrowia lipolytica	35
Exploitation of lignocellulosic-based biomass biorefinery: A critical review of renewable bioresource, sustainability and economic views	35
Refactoring transcription factors for metabolic engineering	35
Utilizing stimuli-responsive nanoparticles to deliver and enhance the anti-tumor effects of bilirubin	34
Prospects on bio-based 2,3-butanediol and acetoin production: Recent progress and advances	34
Editorial Board	34
Cellular variability as a driver for bioprocess innovation and optimization	34
Revolutionizing agroindustry: Towards the industrial application of antimicrobial peptides against pathogens and pests	34
Editorial Board	34
Myxobacteria: Versatile cell factories of novel commercial enzymes for bio-manufacturing	33
Implications of glycosylation for the development of selected cytokines and their derivatives for medical use	33
Strategies to manipulate the performance of aptamers in SELEX, post-SELEX and microenvironment	33
The Yin and Yang of exosome isolation methods: conventional practice, microfluidics, and commercial kits	33
Humans and robots: Friends of the future? A bird's eye view of biomanufacturing industry 5.0	33
The production, recovery, and valorization of polyhydroxybutyrate (PHB) based on circular bioeconomy	33
Microbial extracellular electron transfer and strategies for engineering electroactive microorganisms	33
Grafting of proteins onto polymeric surfaces: A synthesis and characterization challenge	33
Optimization strategies for CO2 biological fixation	32
N-oxygenation of amino compounds: Early stages in its application to the biocatalyzed preparation of bioactive compounds	32
Design and biocatalytic applications of genetically fused multifunctional enzymes	32
Host cell protein-mediated adjuvanticity and immunogenicity risks of biotherapeutics	32
Heparin and heparin-like modifications in hemodialysis membranes: Current innovations and future directions	31
Deciphering mechanisms of production of natural compounds using inducer-producer microbial consortia	31
Molecular biology: Fantastic toolkits to improve knowledge and application of acetic acid bacteria	31
Review of advances in the development of laccases for the valorization of lignin to enable the production of lignocellulosic biofuels and bioproducts	31
Current status and future prospects in cannabinoid production through in vitro culture and synthetic biology	31
From formate oxidation to CO₂ reduction: The role of formate dehydrogenase in sustainable carbon utilization	31
Progress in cyclodextrins as important molecules regulating catalytic processes of glycoside hydrolases	31
Relieving metabolic burden to improve robustness and bioproduction by industrial microorganisms	31
Methods of in vitro study of galectin-glycomaterial interaction	31
Advances in the synthesis of three typical tetraterpenoids including β-carotene, lycopene and astaxanthin	31
Biopolymers and their derivatives: Key components of advanced biomedical technologies	31
Biomass directional pyrolysis based on element economy to produce high-quality fuels, chemicals, carbon materials – A review	31
Lipopeptides development in cosmetics and pharmaceutical applications: A comprehensive review	31
Yeast surface display technology: Mechanisms, applications, and perspectives	30
Strategies for improving antimicrobial peptide production	30
Molecular imprinting technology for biomedical applications	30
Has the time finally come for green oleochemicals and biodiesel production using large-scale enzyme technologies? Current status and new developments	30
Identification, characterization, and engineering of glycosylation in thrombolytics	30
Bacterial membrane vesicle functions, laboratory methods, and applications	30
Editorial Board	29
Editorial Board	29
Nucleic acid-responsive smart systems for controlled cargo delivery	29
A review on the expanding biotechnological frontier of Pedobacter	29
Editorial Board	29
Engineering catalytically promiscuous enzymes to serve new functions	29
Process simulation and evaluation of scaled-up biocatalytic systems: Advances, challenges, and future prospects	28
Recent advances in synthetic biology toolkits and metabolic engineering of Ralstonia eutropha H16 for production of value-added chemicals	28
Strategies for Automated Enzymatic Glycan Synthesis (AEGS)	28
Engineering status of protein for improving microbial cell factories	28
Insights on the emerging biotechnology of histidine-rich peptides	28
Production and characterization of starch-lignin based materials: A review	27
Mycolicibacterium cell factory for the production of steroid-based drug intermediates	27
Antimicrobial peptides: Sustainable application informed by evolutionary constraints	27
Advanced strategies for metabolic engineering of Bacillus to produce extracellular polymeric substances	27
Recent advances in fed-batch microscale bioreactor design	27
Chimeric enzymes in the pulp and paper making industry: Current developments	27
Biophotovoltaics: Recent advances and perspectives	27
Editorial Board	26
4D printing: Perspectives for the production of sustainable plastics for agriculture	26
Nanoantibiotics to fight multidrug resistant infections by Gram-positive bacteria: hope or reality?	26
The new frontier in CHO cell line development: From random to targeted transgene integration technologies	26
Biodegradation of polyethylene and polystyrene: From microbial deterioration to enzyme discovery	26
Cell-culture process optimization via model-based predictions of metabolism and protein glycosylation	26
The role of orphan crops in the transition to nutritional quality-oriented crop improvement	26
Expanding the horizon of transient CAR T therapeutics using virus-free technology	26
Role of distal sites in enzyme engineering	26
Next generation probiotics: Engineering live biotherapeutics	26
Engineering conditional protein-protein interactions for dynamic cellular control	26
Biosynthesis of alkanes/alkenes from fatty acids or derivatives (triacylglycerols or fatty aldehydes)	26
Editorial Board	25
Trends in the development of innovative nanobiocatalysts and their application in biocatalytic transformations	25
Alternative biological sources for extracellular vesicles production and purification strategies for process scale-up	25
Editorial Board	25
Fungal ergot alkaloids: Metabolic pathways, biological functions, and advances in synthetic reprogramming	25
The signaling pathways involved in metabolic regulation and stress responses of the yeast-like fungi Aureobasidium spp.	25
Extremophilic lipases for industrial applications: A general review	25
Current innovations in mRNA vaccines for targeting multidrug-resistant ESKAPE pathogens	24
Agrobacterium strains and strain improvement: Present and outlook	24
Challenges in developing cell culture media using machine learning	24
The vast repertoire of carbohydrate oxidases: An overview	24
Decoding cellular mechanism of recombinant adeno-associated virus (rAAV) and engineering host-cell factories toward intensified viral vector manufacturing	24
Insights to improve the plant nutrient transport by CRISPR/Cas system	24
The evolving art of creating genetic diversity: From directed evolution to synthetic biology	24
Trends in digital image processing of isolated microalgae by incorporating classification algorithm	24
Designing artificial pathways for improving chemical production	24
Biocatalytic production of biolubricants: Strategies, problems and future trends	24