Biotechnology Advances

Article	Citations
Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies	284
Using machine learning approaches for multi-omics data analysis: A review	274
A review of high value-added molecules production by microalgae in light of the classification	272
Microalgae as a future food source	263
Fungal potential for the degradation of petroleum-based polymers: An overview of macro- and microplastics biodegradation	227
The challenge and prospect of mRNA therapeutics landscape	219
The approved gene therapy drugs worldwide: from 1998 to 2019	214
Technologies for biological removal and recovery of nitrogen from wastewater	197
Extracellular matrix-derived biomaterials in engineering cell function	165
Enzyme co-immobilization: Always the biocatalyst designers' choice…or not?	145
Bacterial cellulose micro-nano fibres for wound healing applications	144
Lignin nanoparticles enter the scene: A promising versatile green tool for multiple applications	127
Molecular priming as an approach to induce tolerance against abiotic and oxidative stresses in crop plants	120
Microbial extracellular electron transfer and strategies for engineering electroactive microorganisms	118
Genetic engineering of microalgae for enhanced biorefinery capabilities	116
Molecular farming – The slope of enlightenment	114
Microbial enzymes catalyzing keratin degradation: Classification, structure, function	111
Biotechnological potential and applications of microbial consortia	110
Microbial electrosynthesis: Towards sustainable biorefineries for production of green chemicals from CO2 emissions	110
Biotechnological production of astaxanthin from the microalga Haematococcus pluvialis	109
Biopolymer-liposome hybrid systems for controlled delivery of bioactive compounds: Recent advances	108
Fermentation transforms the phenolic profiles and bioactivities of plant-based foods	108
Purple phototrophic bacteria for resource recovery: Challenges and opportunities	103
Consolidated bio-saccharification: Leading lignocellulose bioconversion into the real world	102
Recent developments in the use of peroxygenases – Exploring their high potential in selective oxyfunctionalisations	102

Algae biostimulants: A critical look at microalgal biostimulants for sustainable agricultural practices	100
Enzymes revolutionize the bioproduction of value-added compounds: From enzyme discovery to special applications	99
Mammalian cell culture for production of recombinant proteins: A review of the critical steps in their biomanufacturing	98
Biodegradation and up-cycling of polyurethanes: Progress, challenges, and prospects	97
Recent advances in systems and synthetic biology approaches for developing novel cell-factories in non-conventional yeasts	94
Potential of phytohormones as a strategy to improve microalgae productivity for biotechnological applications	92
Microbial melanin: Recent advances in biosynthesis, extraction, characterization, and applications	90
Adaptive laboratory evolution principles and applications in industrial biotechnology	86
Prospects and development of algal-bacterial biotechnology in environmental management and protection	85
Library preparation for next generation sequencing: A review of automation strategies	84
Glycoside hydrolase family 18 chitinases: The known and the unknown	82
Biological production of medium-chain carboxylates through chain elongation: An overview	79
Neural tissue engineering with structured hydrogels in CNS models and therapies	78
The invisible life inside plants: Deciphering the riddles of endophytic bacterial diversity	78
Flavoprotein monooxygenases: Versatile biocatalysts	77
Biodegradation of polyethylene and polystyrene: From microbial deterioration to enzyme discovery	76
Expanding the chemical diversity through microorganisms co-culture: Current status and outlook	74
Ecofriendly biopolymers and composites: Preparation and their applications in water-treatment	73
The Yin and Yang of exosome isolation methods: conventional practice, microfluidics, and commercial kits	71
Nanostructured antimicrobial peptides: The last push towards clinics	71
Lipase immobilization on ceramic supports: An overview on techniques and materials	71
Metabolic engineering of Saccharomyces cerevisiae for the production of top value chemicals from biorefinery carbohydrates	69
Vero cell upstream bioprocess development for the production of viral vectors and vaccines	68
Biomaterializing the promise of cardiac tissue engineering	67
Archaea Biotechnology	67
Strategies to manipulate the performance of aptamers in SELEX, post-SELEX and microenvironment	67
Pseudomonas aeruginosa biofilms and their partners in crime	67
Consolidated bioprocessing of raw starch to ethanol by Saccharomyces cerevisiae: Achievements and challenges	65
Developing Aspergillus niger as a cell factory for food enzyme production	64
Iron-assisted biological wastewater treatment: Synergistic effect between iron and microbes	64
Current and novel approaches to downstream processing of microalgae: A review	63
Bacterial cellulose and its potential for biomedical applications	62
Antimicrobial properties and applications of metal nanoparticles biosynthesized by green methods	62
Production of plant natural products through engineered Yarrowia lipolytica	61
Molecularly imprinted polymers for the selective recognition of microorganisms	61
Anthocyanins, multi-functional natural products of industrial relevance: Recent biotechnological advances	61
Current trends in the production of biodegradable bioplastics: The case of polyhydroxyalkanoates	60
Prospects on bio-based 2,3-butanediol and acetoin production: Recent progress and advances	60
Acetate as a potential feedstock for the production of value-added chemicals: Metabolism and applications	58
Biotechnological production of lipid and terpenoid from thraustochytrids	57
Inspired by nature: Microbial production, degradation and valorization of biodegradable bioplastics for life-cycle-engineered products	56
Strategies employed in the design of antimicrobial peptides with enhanced proteolytic stability	56
Microbial production of limonene and its derivatives: Achievements and perspectives	55
Microalgae with artificial intelligence: A digitalized perspective on genetics, systems and products	54
Challenges in biodegradation of non-degradable thermoplastic waste: From environmental impact to operational readiness	54
Alternative sources and metabolic engineering of Taxol: Advances and future perspectives	54
Downstream processing technologies in the biocatalytic production of oligosaccharides	53
Biological and conventional food processing modifications on food proteins: Structure, functionality, and bioactivity	53
Advances in the profiling of N6-methyladenosine (m6A) modifications	53
Genetic engineering of microalgae for enhanced lipid production	52

A comprehensive and critical review on key elements to implement enzymatic PET depolymerization for recycling purposes	52
Biotechnological approaches for the production of natural colorants by Talaromyces/Penicillium: A review	52
Computational design of enzymes for biotechnological applications	51
Boosting extracellular vesicle secretion	50
Review of advances in the development of laccases for the valorization of lignin to enable the production of lignocellulosic biofuels and bioproducts	50
Stress response in Rhodococcus strains	50
Flavonoids, terpenoids, and polyketide antibiotics: Role of glycosylation and biocatalytic tactics in engineering glycosylation	50
Sperm selection in assisted reproduction: A review of established methods and cutting-edge possibilities	49
Understanding gradients in industrial bioreactors	49
Self-assembling peptide-based hydrogels: Fabrication, properties, and applications	49
Whole-cell biocatalysis using cytochrome P450 monooxygenases for biotransformation of sustainable bioresources (fatty acids, fatty alkanes, and aromatic amino acids)	49
A navigation guide of synthetic biology tools for Pseudomonas putida	48
Review: Remotely controlled magneto-regulation of therapeutics from magnetoelastic gel matrices	47
Smart microalgae farming with internet-of-things for sustainable agriculture	47
Mechanical properties of single cells: Measurement methods and applications	46
Polyhydroxyalkanoates synthesis by halophiles and thermophiles: towards sustainable production of microbial bioplastics	46
How can ethanol enhance direct interspecies electron transfer in anaerobic digestion?	45
Bio-synthesis of food additives and colorants-a growing trend in future food	45
Trends in the development of innovative nanobiocatalysts and their application in biocatalytic transformations	45
How does the Internet of Things (IoT) help in microalgae biorefinery?	45
Engineering of Saccharomyces cerevisiae as a consolidated bioprocessing host to produce cellulosic ethanol: Recent advancements and current challenges	44
Production of polyunsaturated fatty acids by Schizochytrium (Aurantiochytrium) spp.	44
Algal metabolites: An inevitable substitute for antibiotics	43
Unlocking the secret of lignin-enzyme interactions: Recent advances in developing state-of-the-art analytical techniques	43
Can anaerobic digestion be a suitable end-of-life scenario for biodegradable plastics? A critical review of the current situation, hurdles, and challenges	42
Biotechnological production and high potential of furan-based renewable monomers and polymers	42
Ionic liquids for regulating biocatalytic process: Achievements and perspectives	42
On the verge of the market – Plant factories for the automated and standardized production of biopharmaceuticals	41
Next-generation metabolic engineering approaches towards development of plant cell suspension cultures as specialized metabolite producing biofactories	41
Recent trends in methane to bioproduct conversion by methanotrophs	41
Biopolymers production from microalgae and cyanobacteria cultivated in wastewater: Recent advances	40
Biotransformation of dietary phytoestrogens by gut microbes: A review on bidirectional interaction between phytoestrogen metabolism and gut microbiota	40
Recent advances in lipid metabolic engineering of oleaginous yeasts	40
What do we learn from enzyme behaviors in organic solvents? – Structural functionalization of ionic liquids for enzyme activation and stabilization	40
Biofilm control by interfering with c-di-GMP metabolism and signaling	40
Amidase as a versatile tool in amide-bond cleavage: From molecular features to biotechnological applications	40
Physical methods for controlling bacterial colonization on polymer surfaces	40
Pathogens and predators impacting commercial production of microalgae and cyanobacteria	39
Microbial starch debranching enzymes: Developments and applications	39
Plant synthetic biology for producing potent phyto-antimicrobials to combat antimicrobial resistance	39
A review on the progress, challenges and prospects in commercializing microalgal fucoxanthin	39
Expansion of human mesenchymal stem/stromal cells (hMSCs) in bioreactors using microcarriers: lessons learnt and what the future holds	38
Biocatalytic oxidation of fatty alcohols into aldehydes for the flavors and fragrances industry	38
Harnessing cells to deliver nanoparticle drugs to treat cancer	38
From bumblebee to bioeconomy: Recent developments and perspectives for sophorolipid biosynthesis	38
Bacterial and enzymatic degradation of poly(cis-1,4-isoprene) rubber: Novel biotechnological applications	38
Waste-to-nutrition: a review of current and emerging conversion pathways	37
Mycolicibacterium cell factory for the production of steroid-based drug intermediates	37
Models of microalgal cultivation for added-value products - A review	37
Engineer probiotic bifidobacteria for food and biomedical applications - Current status and future prospective	37
Extremophilic lipases for industrial applications: A general review	37
Refactoring transcription factors for metabolic engineering	36
Electro-fermentation: Sustainable bioproductions steered by electricity	36
Anodic electro-fermentation: Empowering anaerobic production processes via anodic respiration	36
Marine drugs: Biology, pipelines, current and future prospects for production	36
Insights on the emerging biotechnology of histidine-rich peptides	35
Predicting drug-microbiome interactions with machine learning	35
Hot spots-making directed evolution easier	35
Developing fungal heterologous expression platforms to explore and improve the production of natural products from fungal biodiversity	35
Current status and future prospects in cannabinoid production through in vitro culture and synthetic biology	35
Chemical modification of enzymes to improve biocatalytic performance	34
Biotechnological applications of the non-conventional yeast Meyerozyma guilliermondii	33
From lignocellulose to plastics: Knowledge transfer on the degradation approaches by fungi	33
Non-viral transfection technologies for next-generation therapeutic T cell engineering	33
Adaptive defensive mechanism of bioleaching microorganisms under extremely environmental acid stress: Advances and perspectives	32
Marine macroalgae in a circular economy context: A comprehensive analysis focused on residual biomass	32
Towards efficient enzymatic saccharification of pretreated lignocellulose: Enzyme inhibition by lignin-derived phenolics and recent trends in mitigation strategies	32
Cyanobacteria and microalgae in supporting human habitation on Mars	32
Advances in enzymatic oxyfunctionalization of aliphatic compounds	32
Genetic control of grain appearance quality in rice	31
Common problems associated with the microbial productions of aromatic compounds and corresponding metabolic engineering strategies	31
Engineering whole-cell microbial biosensors: Design principles and applications in monitoring and treatment of heavy metals and organic pollutants	31
Dehydrogenases of acetic acid bacteria	31
Strategies for improving antimicrobial peptide production	31
Microbial-enabled green biosynthesis of nanomaterials: Current status and future prospects	31
Microbial chassis engineering drives heterologous production of complex secondary metabolites	30
Lipid metabolism research in oleaginous fungus Mortierella alpina: Current progress and future prospects	30
Recent advancements and challenges in emerging applications of biochar-based catalysts	30
Phosphorylase-catalyzed bottom-up synthesis of short-chain soluble cello-oligosaccharides and property-tunable cellulosic materials	30
Controlling biofilms using synthetic biology approaches	30

Comparative analysis of the chemical and biochemical synthesis of keto acids	30
Biofilm application in the microbial biochemicals production process	30
The tale of a versatile enzyme: Molecular insights into keratinase for its industrial dissemination	30