Biotechnology Advances

Article	Citations
Use of additives to improve collective biogas plant performances: A comprehensive review	479
Plant secondary metabolites against biotic stresses for sustainable crop protection	415
Advanced technological approaches and market status analysis of xylose bioconversion and utilization: Xylooligosacharides and xylonic acid as emerging products	203
Engineering microbial cell factories by multiplexed spatiotemporal control of cellular metabolism: Advances, challenges, and future perspectives	195
Pathway to industrial application of heterotrophic organisms in critical metals recycling from e-waste	173
Direct cloning strategies for large genomic fragments: A review	167
Process simulation and evaluation of scaled-up biocatalytic systems: Advances, challenges, and future prospects	167
Advancements of astaxanthin production in Haematococcus pluvialis: Update insight and way forward	166
Chimeric enzymes in the pulp and paper making industry: Current developments	151
From discovery to application: Enabling technology-based optimizing carbonyl reductases biocatalysis for active pharmaceutical ingredient synthesis	148
Recent advances in synthetic biology toolkits and metabolic engineering of Ralstonia eutropha H16 for production of value-added chemicals	142
Non-viral engineering of NK cells	132
Editorial Board	123
Employment of the CRISPR/Cas9 system to improve cellulase production in Trichoderma reesei	122
Insights into Clostridium tetani: From genome to bioreactors	113
New opportunities and perspectives on biosynthesis and bioactivities of secondary metabolites from Aloe vera	112
Recent advancements and challenges in emerging applications of biochar-based catalysts	111
Engineering the next-generation synthetic cell factory driven by protein engineering	111
Editorial Board	109
Editorial Board	108
Editorial Board	105
Halocins, natural antimicrobials of Archaea: Exotic or special or both?	103
Cell-culture process optimization via model-based predictions of metabolism and protein glycosylation	95
3D biofabrication and space: A ‘far-fetched dream’ or a ‘forthcoming reality’?	93
Identification, characterization, and engineering of glycosylation in thrombolytics	89

Improving the performance of machine learning models for biotechnology: The quest for deus ex machina	89
Recent advances in human respiratory epithelium models for drug discovery	87
Microbial lifelines in bioprocesses: From concept to application	84
Collagen processing with mesoscale aggregates as templates and building blocks	81
Functional expansion of the natural inorganic phosphorus starvation response system in Escherichia coli	81
Production and characterization of starch-lignin based materials: A review	80
Relevance of extracellular electron uptake mechanisms for electromethanogenesis applications	78
Strategies for Automated Enzymatic Glycan Synthesis (AEGS)	78
The dawning era of oral thin films for nutraceutical delivery: From laboratory to clinic	77
Anaerobic digestion integrated with microbial electrolysis cell to enhance biogas production and upgrading in situ	76
Recent advances in tuning the expression and regulation of genes for constructing microbial cell factories	74
Transcriptomic studies of solventogenic clostridia, Clostridium acetobutylicum and Clostridium beijerinckii	74
Recent developments in animal venom peptide nanotherapeutics with improved selectivity for cancer cells	73
Metabolic and biotransformation effects on dietary glucosinolates, their bioavailability, catabolism and biological effects in different organisms	72
Bacterioferritin nanocage: Structure, biological function, catalytic mechanism, self-assembly and potential applications	72
Nucleic acid-responsive smart systems for controlled cargo delivery	71
Microbial cell surface engineering for high-level synthesis of bio-products	71
Recent advances in the improvement of bi-directional electron transfer between abiotic/biotic interfaces in electron-assisted biosynthesis system	71
An integrated perspective on measuring cytokines to inform CAR-T bioprocessing	70
Unravelling the essential elements for recombinant adeno-associated virus (rAAV) production in animal cell-based platforms	70
Recent advances in culture medium design for enhanced production of monoclonal antibodies in CHO cells: A comparative study of machine learning and systems biology approaches	70
Leveraging independent component analysis to unravel transcriptional regulatory networks: A critical review and future directions	70
Advances and challenges in preparing membrane proteins for native mass spectrometry	69
Anodic electro-fermentation: Empowering anaerobic production processes via anodic respiration	69
Recent advances in fed-batch microscale bioreactor design	68
Manufacturing of bacteriophages for therapeutic applications	68
Biosensor-guided discovery and engineering of metabolic enzymes	65
Transcription factor-based biosensor: A molecular-guided approach for advanced biofuel synthesis	64
Native, engineered and de novo designed ligands targeting the SARS-CoV-2 spike protein	64
Portable biosensors for rapid on-site determination of cannabinoids in cannabis, a review	64
Editorial Board	62
IgG glycans in health and disease: Prediction, intervention, prognosis, and therapy	61
Advanced strategies for metabolic engineering of Bacillus to produce extracellular polymeric substances	61
Flavoprotein monooxygenases: Versatile biocatalysts	61
Recent advances in biocatalysis of nitrogen-containing heterocycles	60
Mycolicibacterium cell factory for the production of steroid-based drug intermediates	59
Data mining of Saccharomyces cerevisiae mutants engineered for increased tolerance towards inhibitors in lignocellulosic hydrolysates	58
Using nanomaterials to increase the efficiency of chemical production in microbial cell factories: A comprehensive review	58
Current status, challenges and prospects for lignin valorization by using Rhodococcus sp.	57
Kluyveromyces marxianus as a microbial cell factory for lignocellulosic biomass valorisation	56
Antimicrobial peptides: Promising alternatives over conventional capture ligands for biosensor-based detection of pathogenic bacteria	56
Role of distal sites in enzyme engineering	56
New trends in biotechnological applications of photosynthetic microorganisms	56
The molecular basis and enzyme engineering strategies for improvement of coupling efficiency in cytochrome P450s	56
New perspectives into Gluconobacter-catalysed biotransformations	55
Evolving perspectives on lutein production from microalgae - A focus on productivity and heterotrophic culture	55
Metabolic engineering of Corynebacterium glutamicum: Unlocking its potential as a key cell factory platform for organic acid production	55
Bacillus licheniformis: The unexplored alternative for the anaerobic production of lipopeptide biosurfactants?	55
Ionic liquids and deep eutectic solvents for the stabilization of biopharmaceuticals: A review	55
Computer-aided understanding and engineering of enzymatic selectivity	54

Biotechnological advances in the production of unusual fatty acids in transgenic plants and recombinant microorganisms	54
Engineering status of protein for improving microbial cell factories	54
Embracing a low-carbon future by the production and marketing of C1 gas protein	54
Biological production of medium-chain carboxylates through chain elongation: An overview	54
Next generation probiotics: Engineering live biotherapeutics	54
The Gompertz model and its applications in microbial growth and bioproduction kinetics: Past, present and future	53
Functional roles and engineering strategies to improve the industrial functionalities of lactic acid bacteria during food fermentation	53
Development of isopentenyl phosphate kinases and their application in terpenoid biosynthesis	53
Biofilm control by interfering with c-di-GMP metabolism and signaling	52
Insights on the emerging biotechnology of histidine-rich peptides	51
Antimicrobial peptides: Sustainable application informed by evolutionary constraints	51
Developments and prospects for doubled haploid wheat	51
Rumen microbes, enzymes, metabolisms, and application in lignocellulosic waste conversion - A comprehensive review	50
Bacterial volatile organic compounds as biopesticides, growth promoters and plant-defense elicitors: Current understanding and future scope	50
Biophotovoltaics: Recent advances and perspectives	49
Carbohydrate flow through agricultural ecosystems: Implications for synthesis and microbial conversion of carbohydrates	49
Comprehensive utilization of sucrose resources via chemical and biotechnological processes: A review	49
Contribution of quorum sensing to virulence and antibiotic resistance in zoonotic bacteria	48
Biohydrogen fermentation from pretreated biomass in lignocellulose biorefinery: Effects of inhibitory byproducts and recent progress in mitigation strategies	47
A comprehensive and critical review on key elements to implement enzymatic PET depolymerization for recycling purposes	46
Biodegradation of polyethylene and polystyrene: From microbial deterioration to enzyme discovery	46
Trait drift in microalgae and applications for strain improvement	46
Biodegradation and up-cycling of polyurethanes: Progress, challenges, and prospects	46
Antimicrobial properties and applications of metal nanoparticles biosynthesized by green methods	46
Bioprospecting for bioactive compounds in microalgae: Antimicrobial compounds	45
Algae biostimulants: A critical look at microalgal biostimulants for sustainable agricultural practices	45
Chiral helical scaffolds: Unlocking their potential in biomolecular interactions and biomedical applications	45
Bacillus sp. as a microbial cell factory: Advancements and future prospects	45
Unleashing the potential of microbial biosynthesis of monoterpenes via enzyme and metabolic engineering	45
Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies	45
Towards the application of nature's catalytic nanomachines: Cellulosomes in 2nd generation biofuel production	45
Lignocellulosic biomass as promising substrate for polyhydroxyalkanoate production: Advances and perspectives	45
An overview of biomethanation and the use of membrane technologies as a candidate to overcome H2 mass transfer limitations	44
Expanding the horizon of transient CAR T therapeutics using virus-free technology	44
Recent advances in the identification of biosynthetic genes and gene clusters of the polyketide-derived pathways for anthraquinone biosynthesis and biotechnological applications	44
Protein representations: Encoding biological information for machine learning in biocatalysis	44
Advances in biosynthesis and downstream processing of diols	44
The new frontier in CHO cell line development: From random to targeted transgene integration technologies	44
ATP regeneration by ATPases for in vitro biotransformation	44
Engineering conditional protein-protein interactions for dynamic cellular control	44
Acetate as a potential feedstock for the production of value-added chemicals: Metabolism and applications	44
Design and application of microfluidics in aptamer SELEX and Aptasensors	44
Editorial Board	43
New trends in industrial biocatalysis	43
Towards engineered yeast as production platform for capsaicinoids	43
Editorial Board	43
Opportunities for visual techniques to determine characteristics and limitations of electro-active biofilms	43
Advances in enzymatic oxyfunctionalization of aliphatic compounds	42
Engineering microbial metabolic energy homeostasis for improved bioproduction	42
Current status, and the developments of hosts and expression systems for the production of recombinant human cytokines	42
Overview of indigo biosynthesis by Flavin-containing Monooxygenases: History, industrialization challenges, and strategies	42
The signaling pathways involved in metabolic regulation and stress responses of the yeast-like fungi Aureobasidium spp.	41
Quality by design to define critical process parameters for mesenchymal stem cell expansion	41
Application of microbial 3-ketosteroid Δ1-dehydrogenases in biotechnology	41
Rugose small colony variant and its hyper-biofilm in Pseudomonas aeruginosa: Adaption, evolution, and biotechnological potential	40
The essential role of mRNA degradation in understanding and engineering E. coli metabolism	39
From bumblebee to bioeconomy: Recent developments and perspectives for sophorolipid biosynthesis	39
Digital models in biotechnology: Towards multi-scale integration and implementation	39
Emerging biotechnologies for evaluating disruption of stress, sleep, and circadian rhythm mechanism using aptamer-based detection of salivary biomarkers	38
From fungal secretomes to enzymes cocktails: The path forward to bioeconomy	38
Stimulated Raman scattering microscopy in chemistry and life science – Development, innovation, perspectives	38
Recent advances in molecular farming using monocot plants	37
Accessing hidden microbial biosynthetic potential from underexplored sources for novel drug discovery	36
Biosensors in microalgae: A roadmap for new opportunities in synthetic biology and biotechnology	36
Phosphorylated polysaccharides: Applications, natural abundance, and new-to-nature structures generated by chemical and enzymatic functionalisation	36
Microbial carbohydrate-binding toxins – From etiology to biotechnological application	36
The role of orphan crops in the transition to nutritional quality-oriented crop improvement	36
Recent advances in design and application of synthetic membraneless organelles	35
Recent advances and discoveries of microbial-based glycolipids: Prospective alternative for remediation activities	35
Enhancing in silico strain design predictions through next generation metabolic modeling approaches	35
Cell wall polysaccharides of streptococci: A genetic and structural perspective	35
Folding of heterologous proteins in bacterial cell factories: Cellular mechanisms and engineering strategies	34
Biopreservation of beer: Potential and constraints	34
Advancing microbial production through artificial intelligence-aided biology	34
Designer phospholipids – structural retrieval, chemo-/bio- synthesis and isotopic labeling	34
Plants arabinogalactans: From structures to physico-chemical and biological properties	33
Recent progress on health effects and biosynthesis of two key sialylated human milk oligosaccharides, 3′-sialyllactose and 6′-sialyllactose	32
Toward biomanufacturing of next-generation bacterial nanocellulose (BNC)-based materials with tailored properties: A review on genetic engineering approaches	32
The thin line between monooxygenases and peroxygenases. P450s, UPOs, MMOs, and LPMOs: A brick to bridge fields of expertise	32
Biobased short chain fatty acid production - Exploring microbial community dynamics and metabolic networks through kinetic and microbial modeling approaches	32

4D printing: Perspectives for the production of sustainable plastics for agriculture	32
Nanoantibiotics to fight multidrug resistant infections by Gram-positive bacteria: hope or reality?	32
Trends in the development of innovative nanobiocatalysts and their application in biocatalytic transformations	31
Optogenetic tools for microbial synthetic biology	31
Recent advances and perspectives on production of value-added organic acids through metabolic engineering	31