Biotechnology Advances

Article	Citations
Editorial Board	446
Development of isopentenyl phosphate kinases and their application in terpenoid biosynthesis	219
Advancements of astaxanthin production in Haematococcus pluvialis: Update insight and way forward	209
Use of additives to improve collective biogas plant performances: A comprehensive review	180
Evolving perspectives on lutein production from microalgae - A focus on productivity and heterotrophic culture	166
Halocins, natural antimicrobials of Archaea: Exotic or special or both?	153
Biotechnological production and emerging applications of betalains: A review	152
Microbial cell surface engineering for high-level synthesis of bio-products	141
New perspectives into Gluconobacter-catalysed biotransformations	135
Recent advances in human respiratory epithelium models for drug discovery	132
Collagen processing with mesoscale aggregates as templates and building blocks	130
Anaerobic digestion integrated with microbial electrolysis cell to enhance biogas production and upgrading in situ	126
Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies	123
Computer-aided understanding and engineering of enzymatic selectivity	122
Chiral helical scaffolds: Unlocking their potential in biomolecular interactions and biomedical applications	120
Accessing hidden microbial biosynthetic potential from underexplored sources for novel drug discovery	118
Think outside the box: 3D bioprinting concepts for biotechnological applications – recent developments and future perspectives	117
Recent advances and perspectives on production of value-added organic acids through metabolic engineering	110
Biosensors in microalgae: A roadmap for new opportunities in synthetic biology and biotechnology	107
Opportunities for visual techniques to determine characteristics and limitations of electro-active biofilms	101
Phosphorylated polysaccharides: Applications, natural abundance, and new-to-nature structures generated by chemical and enzymatic functionalisation	101
Glycosylation in extracellular vesicles: Isolation, characterization, composition, analysis and clinical applications	99
Pickering emulsion biocatalysis: Bridging interfacial design with enzymatic reactions	99
Alpha-ketoacid decarboxylases: Diversity, structures, reaction mechanisms, and applications for biomanufacturing of platform chemicals and fuels	98
Editorial Board	95

Elucidation and biosynthesis of tetrahydroisoquinoline alkaloids: Recent advances and prospects	93
Biotechnological applications of purine and pyrimidine deaminases	92
Enhancing single-cell bioconversion efficiency by harnessing nanosecond pulsed electric field processing	90
Biological valorization of lignin to flavonoids	90
Advances in gene editing-led route for hybrid breeding in crops	89
Methanotrophs as a reservoir for bioactive secondary metabolites: Pitfalls, insights and promises	87
Microbial starch debranching enzymes: Developments and applications	86
Electrode functional microorganisms in bioelectrochemical systems and its regulation: A review	86
Charting the landscape of RNA polymerases to unleash their potential in strain improvement	85
Growth-coupled high throughput selection for directed enzyme evolution	85
Unlocking the secret of lignin-enzyme interactions: Recent advances in developing state-of-the-art analytical techniques	83
Recent advances in machine learning applications in metabolic engineering	82
Ex-situ biomethanation for CO2 valorization: State of the art, recent advances, challenges, and future prospective	82
Haloferax and the Haloferacaceae: Potential role in bioindustry	78
Genome-scale metabolic network models for industrial microorganisms metabolic engineering: Current advances and future prospects	77
Biocatalytic oxyfunctionalization of unsaturated fatty acids to oxygenated chemicals via hydroxy fatty acids	77
From lignocellulose to plastics: Knowledge transfer on the degradation approaches by fungi	75
Polyhydroxyalkanoates synthesis by halophiles and thermophiles: towards sustainable production of microbial bioplastics	73
Emerging advances in glycoengineering of carbohydrates/glycans and their industrial applications	72
Advances in bacterial glycoprotein engineering: A critical review of current technologies, emerging challenges, and future directions	71
Rutinosidase and other diglycosidases: Rising stars in biotechnology	70
Bioinspired nanoplatforms for human-machine interfaces: Recent progress in materials and device applications	70
Recent advances in research for potential utilization of unexplored lichen metabolites	69
Resource allocation in mammalian systems	69
Fusarium as a promising fungal genus with potential application in bioremediation for pollutants mitigation: A review	69
From lignocellulosic biomass to single cell oil for sustainable biomanufacturing: Current advances and prospects	68
Unlocking the bacterial domain for industrial biotechnology applications using universal parts and tools	68
Improving recombinant protein production in CHO cells using the CRISPR-Cas system	68
Utilization of gene manipulation system for advancing the biotechnological potential of halophiles: A review	68
Engineering oleaginous red yeasts as versatile chassis for the production of oleochemicals and valuable compounds: Current advances and perspectives	68
Research progress of engineering microbial cell factories for pigment production	68
Expanding sugar alcohol industry: Microbial production of sugar alcohols and associated chemocatalytic derivatives	65
Problems and corresponding strategies for converting CO2 into value-added products in Cupriavidus necator H16 cell factories	63
Stress response in Rhodococcus strains	63
C-type lectin-(like) fold – Protein-protein interaction patterns and utilization	63
Strategies for overcoming the limitations of enzymatic carbon dioxide reduction	63
Editorial Board	63
Influence of elevated pressure and pressurized fluids on microenvironment and activity of enzymes	63
Cytoskeleton as a roadmap navigating rhizobia to establish symbiotic root nodulation in legumes	63
Capturing the third dimension in drug discovery: Spatially-resolved tools for interrogation of complex 3D cell models	62
Recent advances in enzyme-enhanced immunosensors	61
Detection, production, modification, and application of arylsulfatases	61
Synthetic biology approaches to improve tolerance of inhibitors in lignocellulosic hydrolysates	60
Genetic engineering of bacteriophages: Key concepts, strategies, and applications	60
Recent advances in lipid metabolic engineering of oleaginous yeasts	60
Engineering membrane architecture for biotechnological applications	59
Enzyme co-immobilization: Always the biocatalyst designers' choice…or not?	58
Is it possible to shape the microalgal biomass composition with operational parameters for target compound accumulation?	58
Recent advances in engineering synthetic biomolecular condensates	58
Editorial Board	57

Biological conversion of carbon monoxide and hydrogen by anaerobic culture: Prospect of anaerobic digestion and thermochemical processes combination	57
Corrigendum to “Recent advances in enzyme-enhanced immunosensors” [Biotechnology Advances 53 (2021) 107867].	57
A reflection on the improvement of Chinese hamster ovary cell-based bioprocesses through advances in proteomic techniques	56
Synthetic biology of Fusarium for the sustainable production of valuable bioproducts	56
Ex vivo T cell differentiation in adoptive immunotherapy manufacturing: Critical process parameters and analytical technologies	56
Effect of nanomaterials on microbial metabolism and their applications in fermentative hydrogen production: A review	56
One, two, many: Strategies to alter the number of carbohydrate binding sites of lectins	55
Principles of antibodies with ultralong complementarity-determining regions and picobodies	55
A guide to the use of bioassays in exploration of natural resources	54
Advances in multi-enzyme co-localization strategies for the construction of microbial cell factory	54
Conversion of inulin-rich raw plant biomass to 2,5-furandicarboxylic acid (FDCA): Progress and challenge towards biorenewable plastics	54
Maximizing the potential of nitrilase: Unveiling their diversity, catalytic proficiency, and versatile applications	53
Biotechnological applications of type 1 secretion systems	53
Production of polyunsaturated fatty acids by Schizochytrium (Aurantiochytrium) spp.	53
Functionalized chitosan for cancer nano drug delivery	53
Photocatalyst-enzyme hybrid systems for light-driven biotransformation	52
Optimizing microbial networks through metabolic bypasses	52
Interpretable and explainable predictive machine learning models for data-driven protein engineering	52
Abiotic stress as a dynamic strategy for enhancing high value phytochemicals in microalgae: Critical insights, challenges and future prospects	52
Bacterial cellulose and its potential for biomedical applications	51
The technology, opportunities, and challenges of Synthetic Biological Intelligence	51
Heterologous production of cellulose- and starch-degrading hydrolases to expand Saccharomyces cerevisiae substrate utilization: Lessons learnt	50
Biosynthesis of monoterpenoid and sesquiterpenoid as natural flavors and fragrances	50
Advance computational tools for multiomics data learning	50
Portable biosensors for rapid on-site determination of cannabinoids in cannabis, a review	49
Improving the performance of machine learning models for biotechnology: The quest for deus ex machina	49
Trait drift in microalgae and applications for strain improvement	48
The Gompertz model and its applications in microbial growth and bioproduction kinetics: Past, present and future	48
Data mining of Saccharomyces cerevisiae mutants engineered for increased tolerance towards inhibitors in lignocellulosic hydrolysates	48
Transcriptomic studies of solventogenic clostridia, Clostridium acetobutylicum and Clostridium beijerinckii	48
Metabolic and biotransformation effects on dietary glucosinolates, their bioavailability, catabolism and biological effects in different organisms	48
Rumen microbes, enzymes, metabolisms, and application in lignocellulosic waste conversion - A comprehensive review	48
Bacterioferritin nanocage: Structure, biological function, catalytic mechanism, self-assembly and potential applications	48
Recent advances in tuning the expression and regulation of genes for constructing microbial cell factories	48
A comprehensive and critical review on key elements to implement enzymatic PET depolymerization for recycling purposes	48
Biofilm control by interfering with c-di-GMP metabolism and signaling	48
Biotechnological advances in the production of unusual fatty acids in transgenic plants and recombinant microorganisms	48
Recent advances in the identification of biosynthetic genes and gene clusters of the polyketide-derived pathways for anthraquinone biosynthesis and biotechnological applications	47
Design and application of microfluidics in aptamer SELEX and Aptasensors	47
New trends in the biocatalytic production of nucleosidic active pharmaceutical ingredients using 2′-deoxyribosyltransferases	46
Current status, and the developments of hosts and expression systems for the production of recombinant human cytokines	46
Biopreservation of beer: Potential and constraints	46
Recent advances and discoveries of microbial-based glycolipids: Prospective alternative for remediation activities	45
Protein representations: Encoding biological information for machine learning in biocatalysis	45
Optogenetic technologies in translational cancer research	45
Plants arabinogalactans: From structures to physico-chemical and biological properties	44
From fungal secretomes to enzymes cocktails: The path forward to bioeconomy	44
Plant glycoengineering for designing next-generation vaccines and therapeutic proteins	44
The isolation of lignin with native-like structure	43
Microbial melanin: Recent advances in biosynthesis, extraction, characterization, and applications	43
Challenges and progress towards industrial recombinant protein production in yeasts: A review	42
Microbial gas fermentation technology for sustainable food protein production	42
Strategies for the biological production of ectoine by using different chassis strains	42
Advancements in nanomaterial-based aptasensors for the detection of emerging organic pollutants in environmental and biological samples	41
Recent advances in phyto-combined remediation of heavy metal pollution in soil	41
Strategies to overcome the challenges of low or no expression of heterologous proteins in Escherichia coli	41
Genetically encoded biosensors for microbial synthetic biology: From conceptual frameworks to practical applications	40
CRISPR-Cas13 technology portfolio and alliance with other genetic tools	40
Whole-organism phenotypic screening methods used in early-phase anthelmintic drug discovery	40
Influence of glycosylation on the immunogenicity and antigenicity of viral immunogens	40
Recent advances in microbial production of diamines, aminocarboxylic acids, and diacids as potential platform chemicals and bio-based polyamides monomers	40
Engineered transcription factor-binding diversed functional nucleic acid-based synthetic biosensor	40
Controlled processivity in glycosyltransferases: A way to expand the enzymatic toolbox	40
Ferritin nanocage based delivery vehicles: From single-, co- to compartmentalized- encapsulation of bioactive or nutraceutical compounds	40
Turning waste into treasure: A new direction for low-cost production of lipid chemicals from Thraustochytrids	40
Applications of bio-capacitance to cell culture manufacturing	40
Cupriavidus necator as a platform for polyhydroxyalkanoate production: An overview of strains, metabolism, and modeling approaches	39
Fine-tuning gene expression for improved biosynthesis of natural products: From transcriptional to post-translational regulation	39
Bioinformatics assisted construction of the link between biosynthetic gene clusters and secondary metabolites in fungi	38
The bioproduction of astaxanthin: A comprehensive review on the microbial synthesis and downstream extraction	37
Refactoring transcription factors for metabolic engineering	37
In vitro models of gut digestion across childhood: current developments, challenges and future trends	37
Fluorescence coupling strategies in fluorescence-activated droplet sorting (FADS) for ultrahigh-throughput screening of enzymes, metabolites, and antibodies	37
HEK-omics: The promise of omics to optimize HEK293 for recombinant adeno-associated virus (rAAV) gene therapy manufacturing	37
Recent advances in microbial production of high-value compounds in the tetrapyrrole biosynthesis pathway	37
Advances in production and application technologies of minicells: A review	36
Exploitation of lignocellulosic-based biomass biorefinery: A critical review of renewable bioresource, sustainability and economic views	36
Computational advances in biosynthetic gene cluster discovery and prediction	36
Understanding and controlling the molecular mechanisms of protein aggregation in mAb therapeutics	36
From natural to synthetic: Promoter engineering in yeast expression systems	36

Prospects on bio-based 2,3-butanediol and acetoin production: Recent progress and advances	35
Using oils and fats to replace sugars as feedstocks for biomanufacturing: Challenges and opportunities for the yeast Yarrowia lipolytica	35
Editorial Board	35
Designer bacterial cell factories for improved production of commercially valuable non-ribosomal peptides	35
Editorial Board	35
Selection of aptamers targeting small molecules by capillary electrophoresis: Advances, challenges, and prospects	35