Biotechniques

Papers
(The median citation count of Biotechniques is 2. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2022-08-01 to 2026-08-01.)
ArticleCitations
Ancient DNA Analysis from Epoxy Resin Biodur ® -embedded bones161
Site-Specific Analysis of Ribosomal 2′O-Methylation by Quantitative Reverse Transcription PCR Under Low Deoxynucleotide Triphosphate Concentrations75
UK Biobank: what can it do, how you can use it and how is it being used?28
Decoding Disease: Scott Patterson’s Perspectives on the Power of Biomarkers in Drug Development26
pJoseph2: a family of plasmids as positive controls for bacterial protein expression, transfections, and western blots23
Rectal swab DNA collection protocol for PCR genotyping in rats19
Prioritizing privacy and presentation of supportable hypothesis testing in forensic genetic genealogy investigations18
Correction18
Mito-kaede photoactivation and chase experiment for mitophagy: optimizing flux measurement via fluid exchange system17
Special approach of droplet digital polymerase chain reaction (ddPCR) for transgene stability of a Chinese hamster ovary (CHO) cell line17
Comparison of commercially available DNA and RNA extraction kits for wildlife feces collected from the environment15
Education in Microscopy: Taking a Closer Look with Jennifer Waters14
‘Gel-Stacks’ gently confine or reversibly immobilize arrays of single DNA molecules for manipulation and study14
Comparison of Colorimetric, Spectroscopic and Electrochemical Techniques for Quantification of Hydrogen Sulfide14
An Industry Perspective Approach and Control Strategy for Implementation of Ready-to-Use Cells in Bioassays: Survey Outcome And Recommendations10
Peter and the Beanstalk: Tackling the Giant Questions of Soybean Nodulation10
Gibson Assembly Interposition Improves Amplification Efficiency of Long DNA and Multifragment Overlap Extension PCR10
Enhanced Blastocystis subtyping from stool samples using semi-nested barcode PCR: validation with an NGS-based approach9
Selective Expansion of Target Cells Using the Enrich TROVO Platform9
Three-Dimensional Visualization and Analysis of Dendritic Spines in Human Brain Tissue9
How has CRISPR Transformed Therapeutic Drug Discovery?9
Gel Purification of gDNA for Next-Generation Sequencing Applications8
Who wants to live forever? Models shaping the future of aging research8
A PCR method to detect the mouse major histocompatibility complex H2-L d gen8
Simple, Fast and Inexpensive Hot Sodium Hydroxide and Tris DNA Extraction Method for Genotyping Tomato and Melon Seeds8
Direct-Current Electric Field Stimulation Promotes Proliferation and Maintains Stemness of Mesenchymal Stem Cells8
Integration of mass cytometry and single-cell RNA-sequencing of cells in bronchoalveolar lavage8
Alkaline dip DNA extraction from skin mucus for high-throughput sexing of sterlets ( Acipenser ruthenus )7
When is an SNP not an SNP?7
Accurate Prediction of Serum Antibody Levels from Noninvasive Saliva/Nasal Samples7
A simple and rapid agarose gel electrophoresis method to assess CpG methylation of DNA7
Reference Genes for Gene Expression Profiling in Mouse Models of Listeria Monocytogenes Infection6
Evaluation and Validation of reference Genes in Cymbidium Faberi for real-time Quantitative PCR6
qDATA - an R application implementing a practical framework for analyzing quantitative real-time PCR data6
A Simple Drying Solution that Minimizes Cracking During Air-Drying of Polyacrylamide Gels5
The dynamic duo: cryo-EM teams up with machine learning to visualize biomolecules in motion5
Age is Just a Number: Can Proteomic Analysis Provide Insight into the Aging Body?5
Evaluation and verification of cfDNA extraction methods5
Welcome to the 74th Issue of BioTechniques : 40 years of Publishing the Latest Laboratory Protocols5
Correction5
Self-circularized Overhang-based Unrecombined Products (SOUP): a rapid in vitro method for generating backbone-free expression cassettes5
Cancer’s Sweet Spot: Techniques to Harness Saccharides in Tumor Biology5
Corrigendum5
Freezing diluted bovine serum albumin standards does not significantly affect standard curves5
PS4: A Next-Generation Dataset for Protein Single-Sequence Secondary Structure Prediction4
Dual-mode detection of glucose based on pistol-like DNAzyme-mediated exonuclease-assisted signal cycle4
Assessment of Measurement Accuracy of Amplified Dna Using a Colorimetric Loop-Mediated Isothermal Amplification Assay4
Utilizing the biosimulator to analyze the environmental microbiome within the intensive care units of a hospital4
A simple and rapid in vitro assay for identification of direct inhibitors of O 6 -methylguanine-DNA methyltransferase4
X Marks the Spot: Mapping the Brain with EEG and MRI4
PCR-Based Gene Synthesis with Overlapping Unisense-Oligomers Asymmetric Extension Supported by a Simulator for Oligonucleotide Extension Achieved 1 kbp dsDNA4
3in1: an ultra-fast phage display biopanning method overcoming the biopanning bias and yielding more diverse binders4
Biohacking the Food Chain: Using CRISPR to Combat the Global Food Crisis4
Influence of Resuspension Volume on Dry Sampling Devices Taken for Human Papillomavirus Testing: Implications for Self-Sampling4
Why and when should we choose digital PCR?4
Engineering an Incubation Environment that Mimics in situ Conditions for in vitro coastal microbiome studies4
Western Blotting (immunoblotting): History, Theory, Uses, Protocol and Problems4
A drug screening platform for protein expression levels in neurological disorders4
Investigating the interactomic landscape of survival motor neuron (SMN) and the SMNΔ7 truncated protein4
Importance of a reference ladder in analytical testing of CRISPR HDR donor ssDNA templates4
A Cryostat-Based Frozen Section Method to Increase the Yield of Extracellular Vesicles Extracted from Different Tissues4
Modular probe-based colorimetric miRNA detection via polymerase/endonuclease assisted chain displacement4
Label-free DNA-sensor modelling based on magnetic induction spectroscopy4
Microbial Forensics: What We've Learned from Amerithrax and Beyond4
Crystal Violet Staining is a Reliable Alternative to Bicinchoninic Acid Assay-Based Normalization4
Releasing Secrets Bound to Ancient Remains with Modern DNA Extraction Techniques: An Interview with Elena Essel4
Decoding the Mechanisms of Embryo Development with Spatial Biology4
Developing Virtual and Augmented Reality Applications for Science, Technology, Engineering and Math Education4
Adenovirus Vector System: Construction, History and Therapeutic Applications4
Extraterrestrial agriculture: plant cultivation in space4
Modification of Grocott's Staining Procedure with Heat Treatment and Oxidation by Periodic Acid for Mucormycosis in Tissue: A Method to Detect Mucor spp.4
Methods to Evaluate the Impact of SARS-CoV-2 Nucleocapsid Mutations on Antigen Detection by Rapid Diagnostic Tests4
Optimized for routine: highly sensitive fluorescent Telomeric Repeat Amplification Protocol (f-TRAP)3
A Simplified Procedure for Isolation of Primary Murine Microglia3
Chromatin Immunoprecipitation and Library Preparation: a Powerful Tool to Unravel the Epigenome3
Application of a 3D Bioprinter: Jet Technology For ‘Biopatch’ Development Using Cells on Hydrogel Supports3
Benchmarking antibody discovery fidelity and reproducibility with an assay-locked residue fidelity index3
Fully in vitro iterative construction of a 24 kb-long artificial DNA sequence to store digital information3
Impact of commercial RNA extraction methods on the recovery of human RNA sequence data from archival fixed tissues3
Development of an enhanced anti-pan-N-formylmethionine-specific antibody3
Development and evaluation of a single-tube nested PCR with colorimetric assay for Mycobacterium tuberculosis detection3
Preparation of Bacillus Subtilis Cell Samples and Generation of an SDS-PAGE3
Optimized membrane filtration for high-purity red blood cell isolation from human and murine samples3
Analytical validation of the IBD segment-based tool KinSNP ® for human identification applications3
Nanotechnology and Cancer Therapeutics: Delivering on the Hype?3
A Simple, Sensitive and Colorimetric Assay for Pseudomonas Aeruginosa Infection Analysis3
In vivo RNA targeting in the nematode Caenorhabditis elegans using exogenous catalytic DNA3
Optimization of agarose–gelatin double embedding to minimize sectioning artifacts in multicellular spheroids3
Sampling and analysis methods of air-borne microorganisms in hospital air: a review3
Long-Read Sequencing for the Metagenomic Analysis of Microbiomes3
A HABA dye-based colorimetric assay to detect unoccupied biotin binding sites in an avidin-containing fusion protein3
Transitioning from Triton X-100 to Tergitol 15-S-9: impacts on diagnostic assays using viral PCR sample solution3
Toxoplasma Gondii : The Kitty Litter Parasite3
Strand-specific quantification of L1 ORF0 and related transcripts by multiplex reverse transcription with tagged primers3
Isolation and characterization of Paenibacillus peoriae JC-3jx from Dendrobium nobile3
RNA extraction and RNA-sequencing method for transcriptomic analysis of Mycobacterium tuberculosis3
Spatial proteomics in the age of artificial intelligence: exploring the impact on cancer research3
Specific N-Terminal Amino Acids Potentiate the Periplasmic Expression of Single-Chain Variable Fragments in Escherichia Coli3
Impact of ambient temperature exposure on miRNA stability in human plasma2
Target-Seq: Single Workflow for Detection of Genome Integration Site, DNA Translocation and Off-Target Events2
Artificial intelligence: help or hindrance in solving the reproducibility crisis?2
Amberlite XAD-4 is a Convenient Tool for Removing Triton X-100 and Sarkosyl from Protein Solutions2
Quantitative analysis of oligonucleotide delivery into isolated mitochondria: a proof-of-concept method2
Comparison of Magnetic Bead and Rapid Swab RNA Extraction Methods for Detecting Rabbit Hemorrhagic Disease Virus 2 in Rabbit Liver Samples2
Streamlining single-cell spatial transcriptomics for human kidney tissue2
Stabilization of human RNA and DNA in stool samples at room temperature2
DNA Extraction from Clotted Blood in Genotyping Quality2
I Know What You're Thinking; Can Neuroimaging Truly Reveal Our Innermost Thoughts?2
Quantitative Analysis of lncRNA in Formalin-fixed Paraffin-embedded Tissues of Oral Squamous Cell Carcinoma2
Isolation and profiling of single circulating tumor cells in myeloma: a new workflow for liquid biopsies2
Prediction of High Fecal Propionate-to-Butyrate Ratios Using 16S rRNA-Based Detection of Bacterial Groups with Liquid Array Diagnostics2
Taking root: the techniques growing genetically engineered plants2
Comparison of purification protocols for effective large-primer removal between rounds of PCR amplification2
Pre-sequencing assessment of RNA-Seq library quality using real-time qPCR2
HIV: The Pursuit of an Elusive Vaccine2
The Path to Solving the Protein Folding Problem2
A Technique for the Masses: Single-Cell Analysis Using Mass Cytometry2
Novel buffer for long-term preservation of DNA in biological material at room temperature2
Parameter optimization of NanoJ-SRRF for live-cell microtubule imaging2
A clinical biomarker assay to quantitate CXCL9 in human serum2
Mapping the spread of antibiotic resistance genes in the coastal microbiome2
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