Biotechniques

Article	Citations
UK Biobank: what can it do, how you can use it and how is it being used?	64
Development of a Droplet Digital™ PCR DNA Methylation Detection and Quantification Assay of Prenatal Tobacco Exposure	60
Ancient DNA Analysis from Epoxy Resin Biodur ® -embedded bones	48
Site-Specific Analysis of Ribosomal 2′O-Methylation by Quantitative Reverse Transcription PCR Under Low Deoxynucleotide Triphosphate Concentrations	46
Rectal swab DNA collection protocol for PCR genotyping in rats	41
Mito-kaede photoactivation and chase experiment for mitophagy: optimizing flux measurement via fluid exchange system	26
pJoseph2: a family of plasmids as positive controls for bacterial protein expression, transfections, and western blots	25
Prioritizing privacy and presentation of supportable hypothesis testing in forensic genetic genealogy investigations	20
Decoding Disease: Scott Patterson’s Perspectives on the Power of Biomarkers in Drug Development	18
Correction	16
Comparison of Colorimetric, Spectroscopic and Electrochemical Techniques for Quantification of Hydrogen Sulfide	14
Comparison of commercially available DNA and RNA extraction kits for wildlife feces collected from the environment	14
Welcome to the 72nd Volume of BioTechniques	13
Three-Dimensional Visualization and Analysis of Dendritic Spines in Human Brain Tissue	13
‘Gel-Stacks’ gently confine or reversibly immobilize arrays of single DNA molecules for manipulation and study	13
Enhanced Blastocystis subtyping from stool samples using semi-nested barcode PCR: validation with an NGS-based approach	12
Education in Microscopy: Taking a Closer Look with Jennifer Waters	12
Peter and the Beanstalk: Tackling the Giant Questions of Soybean Nodulation	11
The Forensic Genomics Toolbox is Expanding	11
Gibson Assembly Interposition Improves Amplification Efficiency of Long DNA and Multifragment Overlap Extension PCR	11
An Industry Perspective Approach and Control Strategy for Implementation of Ready-to-Use Cells in Bioassays: Survey Outcome And Recommendations	11
Automated Epidermal Thickness Quantification of in Vitro Human Skin Equivalents Using Optical Coherence Tomography	10
How has CRISPR Transformed Therapeutic Drug Discovery?	10
Who wants to live forever? Models shaping the future of aging research	9
Gel Purification of gDNA for Next-Generation Sequencing Applications	9

Improved SARS-CoV-2 PCR Detection and Genotyping with Double-Bubble Primers	9
Selective Expansion of Target Cells Using the Enrich TROVO Platform	9
Simple, Fast and Inexpensive Hot Sodium Hydroxide and Tris DNA Extraction Method for Genotyping Tomato and Melon Seeds	8
Tergitol-15-S-9 Inactivates SARS-CoV-2 and Boosts Immunoassay Signals	8
Reference Genes for Gene Expression Profiling in Mouse Models of Listeria Monocytogenes Infection	7
qDATA - an R application implementing a practical framework for analyzing quantitative real-time PCR data	7
Direct-Current Electric Field Stimulation Promotes Proliferation and Maintains Stemness of Mesenchymal Stem Cells	7
Evaluation and Validation of reference Genes in Cymbidium Faberi for real-time Quantitative PCR	7
Techniques to Save the Sea	7
High Transfection Efficiency and Cell Viability of Immune Cells with Nanomaterials-Based Transfection Reagent	7
Integration of mass cytometry and single-cell RNA-sequencing of cells in bronchoalveolar lavage	7
Accurate Prediction of Serum Antibody Levels from Noninvasive Saliva/Nasal Samples	6
When is an SNP not an SNP?	6
Yielding Quality Viral RNA by Using Two Different Chemistries: A Comparative Performance Study	6
Alkaline dip DNA extraction from skin mucus for high-throughput sexing of sterlets ( Acipenser ruthenus )	6
Cancer’s Sweet Spot: Techniques to Harness Saccharides in Tumor Biology	6
PCR-Based Gene Synthesis with Overlapping Unisense-Oligomers Asymmetric Extension Supported by a Simulator for Oligonucleotide Extension Achieved 1 kbp dsDNA	5
Modification of Grocott's Staining Procedure with Heat Treatment and Oxidation by Periodic Acid for Mucormycosis in Tissue: A Method to Detect Mucor spp.	5
Corrigendum	5
A Simple Drying Solution that Minimizes Cracking During Air-Drying of Polyacrylamide Gels	5
Freezing diluted bovine serum albumin standards does not significantly affect standard curves	5
Methods to Evaluate the Impact of SARS-CoV-2 Nucleocapsid Mutations on Antigen Detection by Rapid Diagnostic Tests	5
X Marks the Spot: Mapping the Brain with EEG and MRI	5
A Cryostat-Based Frozen Section Method to Increase the Yield of Extracellular Vesicles Extracted from Different Tissues	5
Urgency of Rapid Legionella Detection Post-COVID-19 Lockdowns: an Interview with Elizabeth Minogue	5
Welcome to the 74th Issue of BioTechniques : 40 years of Publishing the Latest Laboratory Protocols	5
Extraterrestrial agriculture: plant cultivation in space	5
Modular probe-based colorimetric miRNA detection via polymerase/endonuclease assisted chain displacement	5
The dynamic duo: cryo-EM teams up with machine learning to visualize biomolecules in motion	5
Ladder-Shape Melting Temperature Isothermal Amplification of Nucleic Acids	5
SUMOylation of the Chromodomain Factor MRG-1 in C. Elegans Affects Chromatin-Regulatory Dynamics	5
A Simplified Method for Bronchoalveolar Lavage in Mice by Orotracheal Intubation Avoiding Tracheotomy	5
Age is Just a Number: Can Proteomic Analysis Provide Insight into the Aging Body?	5
Biohacking the Food Chain: Using CRISPR to Combat the Global Food Crisis	5
Intracellular Flow Cytometry Complements RT-qPCR Detection of Circulating SARS-CoV-2 Variants of Concern	4
Democratizing Bioinformatics through easily Accessible Software Platforms for Non-Experts in the Field	4
Bioinformaticians: the Hidden Heroes of the COVID-19 Pandemic	4
PS4: A Next-Generation Dataset for Protein Single-Sequence Secondary Structure Prediction	4
Apoptosis Assessment in High-Content and High-Throughput Screening Assays	4
Developing Virtual and Augmented Reality Applications for Science, Technology, Engineering and Math Education	4
Importance of a reference ladder in analytical testing of CRISPR HDR donor ssDNA templates	4
Influence of Resuspension Volume on Dry Sampling Devices Taken for Human Papillomavirus Testing: Implications for Self-Sampling	4
Determination of n-3 Index and Arachidonic Acid/eicosapentaenoic Acid Ratio in Dried Blood Spot by Gas Chromatography	4
Simple, Inexpensive and RNase-Free Purification of Plasmid DNA by Fractional Precipitation with Isopropanol	4
Smart PCR Machines Can Reduce the Risk of Carryover Contamination	4
Colonoscopy-Based Intramucosal Transplantation of Cancer Cells for Mouse Modeling of Colon Cancer and Lung Metastasis	4
Who Owns Your DNA?	4
CRISPR-PN2: A Flexible and Genome-Aware Platform for Diverse CRISPR Experiments in Parasitic Nematodes	3
Premature Termination Codon: A Tunable Protein Translation Approach	3
Adenovirus Vector System: Construction, History and Therapeutic Applications	3

Western Blotting (immunoblotting): History, Theory, Uses, Protocol and Problems	3
Releasing Secrets Bound to Ancient Remains with Modern DNA Extraction Techniques: An Interview with Elena Essel	3
Decoding the Mechanisms of Embryo Development with Spatial Biology	3
Crystal Violet Staining is a Reliable Alternative to Bicinchoninic Acid Assay-Based Normalization	3
Cancer Vaccinations: A Personalized Approach	3
Specific N-Terminal Amino Acids Potentiate the Periplasmic Expression of Single-Chain Variable Fragments in Escherichia Coli	3
Engineering an Incubation Environment that Mimics in situ Conditions for in vitro coastal microbiome studies	3
Utilizing the biosimulator to analyze the environmental microbiome within the intensive care units of a hospital	3
A simple and rapid in vitro assay for identification of direct inhibitors of O 6 -methylguanine-DNA methyltransferase	3
Assessment of Measurement Accuracy of Amplified Dna Using a Colorimetric Loop-Mediated Isothermal Amplification Assay	3
Development and evaluation of a single-tube nested PCR with colorimetric assay for Mycobacterium tuberculosis detection	3
A Fast, Nondestructive Method for the Detection of Disease-Related Lesions and Wounded Leaves	3
Microbial Forensics: What We've Learned from Amerithrax and Beyond	3
A drug screening platform for protein expression levels in neurological disorders	3
Psychobiotics: Are we on the Cusp of a Breakthrough?	3
Dual-mode detection of glucose based on pistol-like DNAzyme-mediated exonuclease-assisted signal cycle	3
Development of an enhanced anti-pan-N-formylmethionine-specific antibody	3
Sampling and analysis methods of air-borne microorganisms in hospital air: a review	2
Evaluation Protocol for CRISPR/Cas9-mediated CD19 Knockout GM24385 Cells by Flow Cytometry and Sanger Sequencing	2
Amberlite XAD-4 is a Convenient Tool for Removing Triton X-100 and Sarkosyl from Protein Solutions	2
The Path to Solving the Protein Folding Problem	2
Comparison of Magnetic Bead and Rapid Swab RNA Extraction Methods for Detecting Rabbit Hemorrhagic Disease Virus 2 in Rabbit Liver Samples	2
Prediction of High Fecal Propionate-to-Butyrate Ratios Using 16S rRNA-Based Detection of Bacterial Groups with Liquid Array Diagnostics	2
Nanotechnology and Cancer Therapeutics: Delivering on the Hype?	2
A HABA dye-based colorimetric assay to detect unoccupied biotin binding sites in an avidin-containing fusion protein	2
Application of a 3D Bioprinter: Jet Technology For ‘Biopatch’ Development Using Cells on Hydrogel Supports	2
RNA extraction and RNA-sequencing method for transcriptomic analysis of Mycobacterium tuberculosis	2
Analytical validation of the IBD segment-based tool KinSNP ® for human identification applications	2
Fast Assay to Predict Multipotent Mesenchymal Stromal Cell Replicative Senescence Dynamics	2
Transitioning from Triton X-100 to Tergitol 15-S-9: impacts on diagnostic assays using viral PCR sample solution	2
A Simple Approach for Effective Shearing and Reliable Concentration Measurement of Ultra-High-Molecular-Weight DNA	2
Novel buffer for long-term preservation of DNA in biological material at room temperature	2
Chromatin Immunoprecipitation and Library Preparation: a Powerful Tool to Unravel the Epigenome	2
Effects of a Fully Enclosed Hollow-Fiber Centrifugal Ultrafiltration Technique for Laboratory Biosafety Improvement	2
A Simple, Sensitive and Colorimetric Assay for Pseudomonas Aeruginosa Infection Analysis	2
Optimized for routine: highly sensitive fluorescent Telomeric Repeat Amplification Protocol (f-TRAP)	2
A Simplified Procedure for Isolation of Primary Murine Microglia	2
Long-Read Sequencing for the Metagenomic Analysis of Microbiomes	2
Toxoplasma Gondii : The Kitty Litter Parasite	2
DNA Extraction from Clotted Blood in Genotyping Quality	2
A Technique for the Masses: Single-Cell Analysis Using Mass Cytometry	2
Mapping the spread of antibiotic resistance genes in the coastal microbiome	2
Isolation and characterization of Paenibacillus peoriae JC-3jx from Dendrobium nobile	2
Impact of commercial RNA extraction methods on the recovery of human RNA sequence data from archival fixed tissues	2
Preparation of Bacillus Subtilis Cell Samples and Generation of an SDS-PAGE	2
Fully in vitro iterative construction of a 24 kb-long artificial DNA sequence to store digital information	2
Single-Cell RNA Sequencing of Intramedullary Canal Tissue to Improve Methods for Studying Fracture Repair Biology	2
A single-cell 3D dynamic volume control system for chondrocytes	1
Flow Cytometry: Past and Future	1
How is Spatial Transcriptomics Influencing Cancer Research and Diagnostics?	1
I Know What You're Thinking; Can Neuroimaging Truly Reveal Our Innermost Thoughts?	1
MSCProfiler: A Single Cell Image Processing Workflow to Investigate Mesenchymal Stem Cell Heterogeneity	1
A Simple and Rapid Method for Isolating High-quality RNA from Kenaf with High Polysaccharide and Polyphenol Contents	1
Instant Pot for Antigen Retrieval: A Simple, Safe and Economical Method for Use in Immunohistochemistry	1
Functionalized primer initiated signal cycles and personal glucose meter for sensitive and portable miRNA analysis	1
Artificial intelligence: help or hindrance in solving the reproducibility crisis?	1
Rapid Microfluidic Isolation of Virally Infected Primary Bronchial Epithelial Cells for Single-Cell RNA Sequencing	1
Non-Destructive Extraction of DNA from Preserved Tissues in Medical Collections	1
Target-Seq: Single Workflow for Detection of Genome Integration Site, DNA Translocation and Off-Target Events	1
Endogenous Cell-free DNA in Fetal Bovine Serum Introduces Artifacts to in Vitro cell-free DNA Models	1
Methodology Established for the Detection of Circulating Tumor DNA by Hybridization Capture	1
Development of a Highly Sensitive Nucleic Acid Amplification-Based Detection for Human Leptospirosis Infection	1
Lab Techniques for a More Sustainable World	1
Easy Method for Six-Fragment Golden Gate Assembly of Modular Vectors	1
Efficient production system for hydrogel-based transparent soil for plant root observation	1
Polyacrylamide Gel Electrophoresis: A Versatile Tool for the Separation of Nanoclusters	1
Ancient DNA Sequencing: Telling the Tale of Human History and Evolution	1
Effect of Mismatch Between Types of Viral Nucleic Acid and Intended Targets of Extraction Kits on Polymerase Chain Reaction-Based Testing	1
ncRNAseq: Simple Modifications to RNA-Seq Library Preparation Allow Recovery and Analysis of Mid-Sized Non-Coding RNAs	1
Mini-Thermal Platform Integrated with Microfluidic Device with On-Site Detection for Real-Time DNA Amplification	1
Golden Gate Assembly of BioBrick-Compliant Parts Using Type II Restriction Endonucleases	1
High-Yield Expression of Periplasmic Single-Chain Variable Fragments by Solid Escherichia Coli Cultures	1
Effective Strategies for Isolating DNA from Members of Asteraceae with High Concentrations of Secondary Metabolites	1
Single-cell RNA Sequencing of Bronchoscopy Specimens: Development of a Rapid Minimal Handling Protocol	1
A Building Block-Based Beam-Break (B 5 ) Locomotor Activity Monitoring System and Its Use in Circadian Biology Research	1
Comparison of Optimized Methodologies for Isolating Nuclei from Esophageal Tissue	1
A Comparison of Six DNA Extraction Protocols for 16S, ITS and Shotgun Metagenomic Sequencing of Microbial Communities	1
Advances of Multiplex Ligation-dependent Probe Amplification Technology in Molecular Diagnostics	1
A Simplified and Rapid in Situ Hybridization Protocol for Planarians	1
Special Focus Issue - Celebrating 40 Years of Publishing Lab Techniques: Looking Back to Look Foreword	1

Comparison of Methods for Mapping Rhizosphere Processes in the Context of Their Surrounding Root and Soil Environments	1
EXtraction of Cucumber Phloem Sap Based on the Capillary–Air Pressure Principle	1
The Earth BioGenome Project: A Success Story for Whole-Genome Sequencing?	1
Wheat Improvement Using Genome Editing Technology	1
A Compact and Simple Method of Achieving Differential Transgene Expression by Exploiting Translational Readthrough	1
Generation of UL128 -shRNA Transduced Fibroblasts for the Release Of Cell-free Virus from Clinical Human Cytomegalovirus Isolates	1
Adipocyte ABCA1 expression analysis using flow cytometry	1
Extracting High-Quality RNA From Formaldehyde-Fixed Naturally Aged Neuromusculoskeletal Tissues	1
Cassava for the future: embryogenic liquid cultures suitable for new biotech techniques	1
Magnetic Tweezers: Development and Use in Single-Molecule Research	1
Corrigendum	1
Mito-Kaede photoactivation and chase experiment for mitophagy: mitophagy flux response toward various stimulations	1
Considerations for Developing Mitochondrial Transplantation Techniques for Individualized Medicine	1
Rapid Golden Gate assembly of exons from genomic DNA for protein expression in Escherichia coli and Pichia Pastoris	1
Accurate, Fast and Cost-Effective Simultaneous Detection of Bacterial Meningitis by Qualitative PCR with High-Resolution Melting	1
Considerations for Colorblind Individuals on Selecting Colorimetric or Fluorescent Dye Assay Outcomes	1
Cytotoxic T Lymphocytes Targeting a Conserved SARS-CoV-2 Spike Epitope are Efficient Serial Killers	1
Cradle Cultures: Growing Stem Cell-Derived Developmental Cell Models in Vitro	1
Legionella Water Testing and the EU Drinking Water Directive: Could Potentially Harmful Legionella Bacteria Slip Through the Gaps?	1
Sample Collection/Stabilization and DNA/RNA Extraction from Swab Samples for Microbiome or Metagenome Analyses	1
HIV: The Pursuit of an Elusive Vaccine	1
An Optimal Genomic Dna Extraction Method for Shoots of Four Dendrocalamus Species Based on Membership Function Analysis	1
Sociometric Wearable Devices for Studying Human Behavior in Corporate and Healthcare Workplaces	1
Quantitative Analysis of lncRNA in Formalin-fixed Paraffin-embedded Tissues of Oral Squamous Cell Carcinoma	1
Colorimetric Analysis of C-reactive Protein Via ‘Jellyfish’ Probe-based Exonuclease III-assisted Multiple-Signal Recycles	1
Optimized RNA Isolation of FFPE Uterine Scar Tissues for RNA Expression Analyses Delineated by Laser Microdissection	1
Characterization of nuclear microsatellites in Marchantia polymorpha (liverwort) with additional trans-specific analyses	1
Metagenomic Nanopore Sequencing Versus Conventional Diagnosis for Identification of the Dieback Pathogens of Mango Trees	1
Development of a human tyrosinase activity inhibition assay using human melanoma cell lysate	1
Recording the Brain in Vivo : Emerging Technologies for the Exploration of Mental Health Conditions	1
Removal of Nonspecific Binding Proteins is Required in Co-Immunoprecipitation with Nuclear Proteins	1