Biotechniques

Article	Citations
UK Biobank: what can it do, how you can use it and how is it being used?	70
Development of a Droplet Digital™ PCR DNA Methylation Detection and Quantification Assay of Prenatal Tobacco Exposure	69
Site-Specific Analysis of Ribosomal 2′O-Methylation by Quantitative Reverse Transcription PCR Under Low Deoxynucleotide Triphosphate Concentrations	55
Ancient DNA Analysis from Epoxy Resin Biodur ® -embedded bones	50
Rectal swab DNA collection protocol for PCR genotyping in rats	44
Mito-kaede photoactivation and chase experiment for mitophagy: optimizing flux measurement via fluid exchange system	30
pJoseph2: a family of plasmids as positive controls for bacterial protein expression, transfections, and western blots	20
Decoding Disease: Scott Patterson’s Perspectives on the Power of Biomarkers in Drug Development	19
Correction	17
Comparison of commercially available DNA and RNA extraction kits for wildlife feces collected from the environment	16
Welcome to the 72nd Volume of BioTechniques	15
Prioritizing privacy and presentation of supportable hypothesis testing in forensic genetic genealogy investigations	15
‘Gel-Stacks’ gently confine or reversibly immobilize arrays of single DNA molecules for manipulation and study	14
Enhanced Blastocystis subtyping from stool samples using semi-nested barcode PCR: validation with an NGS-based approach	13
Education in Microscopy: Taking a Closer Look with Jennifer Waters	13
Three-Dimensional Visualization and Analysis of Dendritic Spines in Human Brain Tissue	13
Gibson Assembly Interposition Improves Amplification Efficiency of Long DNA and Multifragment Overlap Extension PCR	12
Peter and the Beanstalk: Tackling the Giant Questions of Soybean Nodulation	12
The Forensic Genomics Toolbox is Expanding	11
An Industry Perspective Approach and Control Strategy for Implementation of Ready-to-Use Cells in Bioassays: Survey Outcome And Recommendations	11
Comparison of Colorimetric, Spectroscopic and Electrochemical Techniques for Quantification of Hydrogen Sulfide	11
Gel Purification of gDNA for Next-Generation Sequencing Applications	10
How has CRISPR Transformed Therapeutic Drug Discovery?	10
Improved SARS-CoV-2 PCR Detection and Genotyping with Double-Bubble Primers	9
Simple, Fast and Inexpensive Hot Sodium Hydroxide and Tris DNA Extraction Method for Genotyping Tomato and Melon Seeds	9

Selective Expansion of Target Cells Using the Enrich TROVO Platform	9
Who wants to live forever? Models shaping the future of aging research	9
Automated Epidermal Thickness Quantification of in Vitro Human Skin Equivalents Using Optical Coherence Tomography	8
Integration of mass cytometry and single-cell RNA-sequencing of cells in bronchoalveolar lavage	8
Direct-Current Electric Field Stimulation Promotes Proliferation and Maintains Stemness of Mesenchymal Stem Cells	8
Techniques to Save the Sea	7
High Transfection Efficiency and Cell Viability of Immune Cells with Nanomaterials-Based Transfection Reagent	7
Evaluation and Validation of reference Genes in Cymbidium Faberi for real-time Quantitative PCR	7
Alkaline dip DNA extraction from skin mucus for high-throughput sexing of sterlets ( Acipenser ruthenus )	7
Accurate Prediction of Serum Antibody Levels from Noninvasive Saliva/Nasal Samples	7
Yielding Quality Viral RNA by Using Two Different Chemistries: A Comparative Performance Study	6
Urgency of Rapid Legionella Detection Post-COVID-19 Lockdowns: an Interview with Elizabeth Minogue	6
Corrigendum	6
Cancer’s Sweet Spot: Techniques to Harness Saccharides in Tumor Biology	6
Reference Genes for Gene Expression Profiling in Mouse Models of Listeria Monocytogenes Infection	6
SUMOylation of the Chromodomain Factor MRG-1 in C. Elegans Affects Chromatin-Regulatory Dynamics	6
When is an SNP not an SNP?	6
qDATA - an R application implementing a practical framework for analyzing quantitative real-time PCR data	6
A Simple Drying Solution that Minimizes Cracking During Air-Drying of Polyacrylamide Gels	6
A Simplified Method for Bronchoalveolar Lavage in Mice by Orotracheal Intubation Avoiding Tracheotomy	5
Modification of Grocott's Staining Procedure with Heat Treatment and Oxidation by Periodic Acid for Mucormycosis in Tissue: A Method to Detect Mucor spp.	5
X Marks the Spot: Mapping the Brain with EEG and MRI	5
Modular probe-based colorimetric miRNA detection via polymerase/endonuclease assisted chain displacement	5
Welcome to the 74th Issue of BioTechniques : 40 years of Publishing the Latest Laboratory Protocols	5
Extraterrestrial agriculture: plant cultivation in space	5
Methods to Evaluate the Impact of SARS-CoV-2 Nucleocapsid Mutations on Antigen Detection by Rapid Diagnostic Tests	5
The dynamic duo: cryo-EM teams up with machine learning to visualize biomolecules in motion	5
Freezing diluted bovine serum albumin standards does not significantly affect standard curves	5
Age is Just a Number: Can Proteomic Analysis Provide Insight into the Aging Body?	5
Biohacking the Food Chain: Using CRISPR to Combat the Global Food Crisis	5
A Cryostat-Based Frozen Section Method to Increase the Yield of Extracellular Vesicles Extracted from Different Tissues	5
Simple, Inexpensive and RNase-Free Purification of Plasmid DNA by Fractional Precipitation with Isopropanol	4
Importance of a reference ladder in analytical testing of CRISPR HDR donor ssDNA templates	4
Colonoscopy-Based Intramucosal Transplantation of Cancer Cells for Mouse Modeling of Colon Cancer and Lung Metastasis	4
PCR-Based Gene Synthesis with Overlapping Unisense-Oligomers Asymmetric Extension Supported by a Simulator for Oligonucleotide Extension Achieved 1 kbp dsDNA	4
Democratizing Bioinformatics through easily Accessible Software Platforms for Non-Experts in the Field	4
PS4: A Next-Generation Dataset for Protein Single-Sequence Secondary Structure Prediction	4
Adenovirus Vector System: Construction, History and Therapeutic Applications	4
Developing Virtual and Augmented Reality Applications for Science, Technology, Engineering and Math Education	4
Determination of n-3 Index and Arachidonic Acid/eicosapentaenoic Acid Ratio in Dried Blood Spot by Gas Chromatography	4
Bioinformaticians: the Hidden Heroes of the COVID-19 Pandemic	4
Influence of Resuspension Volume on Dry Sampling Devices Taken for Human Papillomavirus Testing: Implications for Self-Sampling	4
A simple and rapid in vitro assay for identification of direct inhibitors of O 6 -methylguanine-DNA methyltransferase	4
Intracellular Flow Cytometry Complements RT-qPCR Detection of Circulating SARS-CoV-2 Variants of Concern	4
Smart PCR Machines Can Reduce the Risk of Carryover Contamination	4
Engineering an Incubation Environment that Mimics in situ Conditions for in vitro coastal microbiome studies	3
Crystal Violet Staining is a Reliable Alternative to Bicinchoninic Acid Assay-Based Normalization	3
Decoding the Mechanisms of Embryo Development with Spatial Biology	3
Dual-mode detection of glucose based on pistol-like DNAzyme-mediated exonuclease-assisted signal cycle	3
Application of a 3D Bioprinter: Jet Technology For ‘Biopatch’ Development Using Cells on Hydrogel Supports	3

Single-Cell RNA Sequencing of Intramedullary Canal Tissue to Improve Methods for Studying Fracture Repair Biology	3
Analytical validation of the IBD segment-based tool KinSNP ® for human identification applications	3
Fast Assay to Predict Multipotent Mesenchymal Stromal Cell Replicative Senescence Dynamics	3
Premature Termination Codon: A Tunable Protein Translation Approach	3
Psychobiotics: Are we on the Cusp of a Breakthrough?	3
Releasing Secrets Bound to Ancient Remains with Modern DNA Extraction Techniques: An Interview with Elena Essel	3
A drug screening platform for protein expression levels in neurological disorders	3
Assessment of Measurement Accuracy of Amplified Dna Using a Colorimetric Loop-Mediated Isothermal Amplification Assay	3
Microbial Forensics: What We've Learned from Amerithrax and Beyond	3
Optimized for routine: highly sensitive fluorescent Telomeric Repeat Amplification Protocol (f-TRAP)	3
Preparation of Bacillus Subtilis Cell Samples and Generation of an SDS-PAGE	3
Long-Read Sequencing for the Metagenomic Analysis of Microbiomes	3
Development and evaluation of a single-tube nested PCR with colorimetric assay for Mycobacterium tuberculosis detection	3
Western Blotting (immunoblotting): History, Theory, Uses, Protocol and Problems	3
A Fast, Nondestructive Method for the Detection of Disease-Related Lesions and Wounded Leaves	3
Utilizing the biosimulator to analyze the environmental microbiome within the intensive care units of a hospital	3
CRISPR-PN2: A Flexible and Genome-Aware Platform for Diverse CRISPR Experiments in Parasitic Nematodes	3
Specific N-Terminal Amino Acids Potentiate the Periplasmic Expression of Single-Chain Variable Fragments in Escherichia Coli	3
A HABA dye-based colorimetric assay to detect unoccupied biotin binding sites in an avidin-containing fusion protein	3
A Simplified Procedure for Isolation of Primary Murine Microglia	3
Development of an enhanced anti-pan-N-formylmethionine-specific antibody	3
Mapping the spread of antibiotic resistance genes in the coastal microbiome	2
Novel buffer for long-term preservation of DNA in biological material at room temperature	2
Nanotechnology and Cancer Therapeutics: Delivering on the Hype?	2
Fully in vitro iterative construction of a 24 kb-long artificial DNA sequence to store digital information	2
Sampling and analysis methods of air-borne microorganisms in hospital air: a review	2
Impact of commercial RNA extraction methods on the recovery of human RNA sequence data from archival fixed tissues	2
Quantitative Analysis of lncRNA in Formalin-fixed Paraffin-embedded Tissues of Oral Squamous Cell Carcinoma	2
A Simplified and Rapid in Situ Hybridization Protocol for Planarians	2
Target-Seq: Single Workflow for Detection of Genome Integration Site, DNA Translocation and Off-Target Events	2
Cradle Cultures: Growing Stem Cell-Derived Developmental Cell Models in Vitro	2
DNA Extraction from Clotted Blood in Genotyping Quality	2
Prediction of High Fecal Propionate-to-Butyrate Ratios Using 16S rRNA-Based Detection of Bacterial Groups with Liquid Array Diagnostics	2
Comparison of Magnetic Bead and Rapid Swab RNA Extraction Methods for Detecting Rabbit Hemorrhagic Disease Virus 2 in Rabbit Liver Samples	2
Evaluation Protocol for CRISPR/Cas9-mediated CD19 Knockout GM24385 Cells by Flow Cytometry and Sanger Sequencing	2
RNA extraction and RNA-sequencing method for transcriptomic analysis of Mycobacterium tuberculosis	2
Toxoplasma Gondii : The Kitty Litter Parasite	2
A Technique for the Masses: Single-Cell Analysis Using Mass Cytometry	2
Artificial intelligence: help or hindrance in solving the reproducibility crisis?	2
I Know What You're Thinking; Can Neuroimaging Truly Reveal Our Innermost Thoughts?	2
Development of a Highly Sensitive Nucleic Acid Amplification-Based Detection for Human Leptospirosis Infection	2
The Path to Solving the Protein Folding Problem	2
Effects of a Fully Enclosed Hollow-Fiber Centrifugal Ultrafiltration Technique for Laboratory Biosafety Improvement	2
Isolation and characterization of Paenibacillus peoriae JC-3jx from Dendrobium nobile	2
Transitioning from Triton X-100 to Tergitol 15-S-9: impacts on diagnostic assays using viral PCR sample solution	2
Chromatin Immunoprecipitation and Library Preparation: a Powerful Tool to Unravel the Epigenome	2
A Simple, Sensitive and Colorimetric Assay for Pseudomonas Aeruginosa Infection Analysis	2
Amberlite XAD-4 is a Convenient Tool for Removing Triton X-100 and Sarkosyl from Protein Solutions	2
Parameter optimization of NanoJ-SRRF for live-cell microtubule imaging	2
HIV: The Pursuit of an Elusive Vaccine	2
EXtraction of Cucumber Phloem Sap Based on the Capillary–Air Pressure Principle	2
A Compact and Simple Method of Achieving Differential Transgene Expression by Exploiting Translational Readthrough	2
Colorimetric Analysis of C-reactive Protein Via ‘Jellyfish’ Probe-based Exonuclease III-assisted Multiple-Signal Recycles	1
Comparison of Optimized Methodologies for Isolating Nuclei from Esophageal Tissue	1
A Building Block-Based Beam-Break (B 5 ) Locomotor Activity Monitoring System and Its Use in Circadian Biology Research	1
Legionella Water Testing and the EU Drinking Water Directive: Could Potentially Harmful Legionella Bacteria Slip Through the Gaps?	1
Metagenomic Nanopore Sequencing Versus Conventional Diagnosis for Identification of the Dieback Pathogens of Mango Trees	1
An Optimized TRIzol-Based Method for Isolating RNA from Adipose Tissue	1
Removal of Nonspecific Binding Proteins is Required in Co-Immunoprecipitation with Nuclear Proteins	1
Cassava for the future: embryogenic liquid cultures suitable for new biotech techniques	1
Magnetic Tweezers: Development and Use in Single-Molecule Research	1
Ancient DNA Sequencing: Telling the Tale of Human History and Evolution	1
An enzyme-free alcohol-based organoid harvesting solution	1
Optimized RNA Isolation of FFPE Uterine Scar Tissues for RNA Expression Analyses Delineated by Laser Microdissection	1
Cytotoxic T Lymphocytes Targeting a Conserved SARS-CoV-2 Spike Epitope are Efficient Serial Killers	1
Non-Destructive Extraction of DNA from Preserved Tissues in Medical Collections	1
Instant Pot for Antigen Retrieval: A Simple, Safe and Economical Method for Use in Immunohistochemistry	1
Flow Cytometry: Past and Future	1
Considerations for Colorblind Individuals on Selecting Colorimetric or Fluorescent Dye Assay Outcomes	1
Effective Strategies for Isolating DNA from Members of Asteraceae with High Concentrations of Secondary Metabolites	1
Adipocyte ABCA1 expression analysis using flow cytometry	1
Efficient production system for hydrogel-based transparent soil for plant root observation	1
Mini-Thermal Platform Integrated with Microfluidic Device with On-Site Detection for Real-Time DNA Amplification	1
Special Focus Issue - Celebrating 40 Years of Publishing Lab Techniques: Looking Back to Look Foreword	1
The Earth BioGenome Project: A Success Story for Whole-Genome Sequencing?	1
Considerations for Developing Mitochondrial Transplantation Techniques for Individualized Medicine	1
Advances of Multiplex Ligation-dependent Probe Amplification Technology in Molecular Diagnostics	1
High-Yield Expression of Periplasmic Single-Chain Variable Fragments by Solid Escherichia Coli Cultures	1
A Simple and Economical Site-Directed Mutagenesis Method for Large Plasmids by Direct Transformation of two Overlapping PCR Fragments	1
A novel device for buffy coat collection	1

Recording the Brain in Vivo : Emerging Technologies for the Exploration of Mental Health Conditions	1
A single-cell 3D dynamic volume control system for chondrocytes	1
Golden Gate Assembly of BioBrick-Compliant Parts Using Type II Restriction Endonucleases	1
ncRNAseq: Simple Modifications to RNA-Seq Library Preparation Allow Recovery and Analysis of Mid-Sized Non-Coding RNAs	1
Methodology Established for the Detection of Circulating Tumor DNA by Hybridization Capture	1
Corrigendum	1
Sample Collection/Stabilization and DNA/RNA Extraction from Swab Samples for Microbiome or Metagenome Analyses	1
Polyacrylamide Gel Electrophoresis: A Versatile Tool for the Separation of Nanoclusters	1
Comparison of Methods for Mapping Rhizosphere Processes in the Context of Their Surrounding Root and Soil Environments	1
Rapid Golden Gate assembly of exons from genomic DNA for protein expression in Escherichia coli and Pichia Pastoris	1
A Comparison of Six DNA Extraction Protocols for 16S, ITS and Shotgun Metagenomic Sequencing of Microbial Communities	1
Generation of UL128 -shRNA Transduced Fibroblasts for the Release Of Cell-free Virus from Clinical Human Cytomegalovirus Isolates	1
A Simple and Rapid Method for Isolating High-quality RNA from Kenaf with High Polysaccharide and Polyphenol Contents	1
Accurate, Fast and Cost-Effective Simultaneous Detection of Bacterial Meningitis by Qualitative PCR with High-Resolution Melting	1
An Optimal Genomic Dna Extraction Method for Shoots of Four Dendrocalamus Species Based on Membership Function Analysis	1
Wheat Improvement Using Genome Editing Technology	1
Extracting High-Quality RNA From Formaldehyde-Fixed Naturally Aged Neuromusculoskeletal Tissues	1
Validation of CRISPR Targeting for Proliferation and Cytarabine Resistance Control Genes in the Acute Myeloid Leukemia Cell Line MOLM-13	1
Quantitative ELISA for SERPINA4/kallistatin	1
Effect of Mismatch Between Types of Viral Nucleic Acid and Intended Targets of Extraction Kits on Polymerase Chain Reaction-Based Testing	1
Endogenous Cell-free DNA in Fetal Bovine Serum Introduces Artifacts to in Vitro cell-free DNA Models	1
Dual compartment utility of BRET-based biosensors for PPP2R5A/B56α, a cancer-associated B regulatory subunit of PP2A	1
Mito-Kaede photoactivation and chase experiment for mitophagy: mitophagy flux response toward various stimulations	1
Selection and Validation of Reference Genes for Quantitative Real-Time PCR in Cymbidium Sinense	1
Easy Method for Six-Fragment Golden Gate Assembly of Modular Vectors	1
Lab Techniques for a More Sustainable World	1
Development of a human tyrosinase activity inhibition assay using human melanoma cell lysate	1
Functionalized primer initiated signal cycles and personal glucose meter for sensitive and portable miRNA analysis	1
MSCProfiler: A Single Cell Image Processing Workflow to Investigate Mesenchymal Stem Cell Heterogeneity	1
Characterization of nuclear microsatellites in Marchantia polymorpha (liverwort) with additional trans-specific analyses	1
Single-cell RNA Sequencing of Bronchoscopy Specimens: Development of a Rapid Minimal Handling Protocol	1
How is Spatial Transcriptomics Influencing Cancer Research and Diagnostics?	1