Biotechniques

Article	Citations
The dynamic duo: cryo-EM teams up with machine learning to visualize biomolecules in motion	57
CRISPR screens in neurological research: exploring the functional basis of aging and disease	48
A Clinically Validated Human Saliva Metatranscriptomic Test for Global Systems Biology Studies	42
Ladder-Shape Melting Temperature Isothermal Amplification of Nucleic Acids	39
Special Focus Issue - Celebrating 40 Years of Publishing Lab Techniques: Looking Back to Look Foreword	37
Unraveling Crime Scenes Strand by Strand: The Forensic Odyssey of Bruce Budowle	24
Phylobook: a tool for display, clade annotation and extraction of sequences from molecular phylogenies	23
Characterization of nuclear microsatellites in Marchantia polymorpha (liverwort) with additional trans-specific analyses	19
Development and validation of a portable device for lab-free versatile nucleic acid extraction	19
UK Biobank: what can it do, how you can use it and how is it being used?	16
A Novel Click Chemistry-based Peptide ELISA Protocol: Development and Technical Evaluation	15
Isolation and characterization of Paenibacillus peoriae JC-3jx from Dendrobium nobile	14
Extraterrestrial agriculture: plant cultivation in space	13
Recombinant Bacterial Endotoxin Testing: A Proven Solution	12
Digital Holographic Microscopy: A Noninvasive Method to Analyze the Formation of Spheroids	12
Transitioning from Triton X-100 to Tergitol 15-S-9: impacts on diagnostic assays using viral PCR sample solution	12
Intracellular Flow Cytometry Complements RT-qPCR Detection of Circulating SARS-CoV-2 Variants of Concern	11
An Approach to Identifying Quality Research Antibodies	11
Ancient DNA Analysis from Epoxy Resin Biodur ® -embedded bones	10
Effects of a Fully Enclosed Hollow-Fiber Centrifugal Ultrafiltration Technique for Laboratory Biosafety Improvement	10
Legionella Water Testing and the EU Drinking Water Directive: Could Potentially Harmful Legionella Bacteria Slip Through the Gaps?	9
Toxoplasma Gondii : The Kitty Litter Parasite	9
Rapid Microfluidic Isolation of Virally Infected Primary Bronchial Epithelial Cells for Single-Cell RNA Sequencing	9
Biohacking the Food Chain: Using CRISPR to Combat the Global Food Crisis	9
X Marks the Spot: Mapping the Brain with EEG and MRI	9

Ancient DNA Sequencing: Telling the Tale of Human History and Evolution	9
Chromatin Immunoprecipitation and Library Preparation: a Powerful Tool to Unravel the Epigenome	8
Site-Specific Analysis of Ribosomal 2′O-Methylation by Quantitative Reverse Transcription PCR Under Low Deoxynucleotide Triphosphate Concentrations	8
Adipocyte ABCA1 expression analysis using flow cytometry	7
Age is Just a Number: Can Proteomic Analysis Provide Insight into the Aging Body?	7
An Easy Method for Quantification of Anaerobic and Microaerobic Gene Expression with Fluorescent Reporter Proteins	7
Modular probe-based colorimetric miRNA detection via polymerase/endonuclease assisted chain displacement	7
A Rapid and Sensitive Method to Measure Numbers of Live Cells in Alginate Capsules Following Depolymerization with Ethylenediaminetetraacetic Acid	7
Methods to Evaluate the Impact of SARS-CoV-2 Nucleocapsid Mutations on Antigen Detection by Rapid Diagnostic Tests	7
Nanotechnology and Cancer Therapeutics: Delivering on the Hype?	7
Adaptation of the HEp-2 Cell Line to toTally Animal-Free Culture Systems and Real-Time Analysis of Cell Growth	6
Multiparameter Screen Optimizes Immunoprecipitation	6
A Practical Approach to Genome Assembly and Annotation of Basidiomycota Using the Example of Armillaria	6
Generation of UL128 -shRNA Transduced Fibroblasts for the Release Of Cell-free Virus from Clinical Human Cytomegalovirus Isolates	6
Modification of Grocott's Staining Procedure with Heat Treatment and Oxidation by Periodic Acid for Mucormycosis in Tissue: A Method to Detect Mucor spp.	6
An Optimal Genomic Dna Extraction Method for Shoots of Four Dendrocalamus Species Based on Membership Function Analysis	6
Apoptosis Assessment in High-Content and High-Throughput Screening Assays	6
A Cryostat-Based Frozen Section Method to Increase the Yield of Extracellular Vesicles Extracted from Different Tissues	6
PCR-Based Gene Synthesis with Overlapping Unisense-Oligomers Asymmetric Extension Supported by a Simulator for Oligonucleotide Extension Achieved 1 kbp dsDNA	5
Smart PCR Machines Can Reduce the Risk of Carryover Contamination	5
Development of a Droplet Digital™ PCR DNA Methylation Detection and Quantification Assay of Prenatal Tobacco Exposure	5
A Simple Approach for Effective Shearing and Reliable Concentration Measurement of Ultra-High-Molecular-Weight DNA	5
Evaluation Protocol for CRISPR/Cas9-mediated CD19 Knockout GM24385 Cells by Flow Cytometry and Sanger Sequencing	5
Isothermal Amplification Using Sequence-Specific Fluorescence Detection of SARS Coronavirus 2 and Variants in Nasal Swabs	5
Comparing robotic and manual injection methods in zebrafish embryos for high-throughput RNA silencing using CRISPR-RfxCas13d	5
Cloning Vectors for Gene Delivery, Integration and Expression in Campylobacter Jejuni	4
A standardized protocol for sample preparation for scanning electron microscopy to visualize extrachromosomal DNA	4
Comparison of Magnetic Bead and Rapid Swab RNA Extraction Methods for Detecting Rabbit Hemorrhagic Disease Virus 2 in Rabbit Liver Samples	4
Lab Techniques for a More Sustainable World	4
CRISPR in 3D: Innovations in Disease Modelling and Personalized Medicine	4
Amberlite XAD-4 is a Convenient Tool for Removing Triton X-100 and Sarkosyl from Protein Solutions	4
Prioritizing privacy and presentation of supportable hypothesis testing in forensic genetic genealogy investigations	4
Polyacrylamide Gel Electrophoresis: A Versatile Tool for the Separation of Nanoclusters	4
Colorimetric Analysis of C-reactive Protein Via ‘Jellyfish’ Probe-based Exonuclease III-assisted Multiple-Signal Recycles	4
Novel buffer for long-term preservation of DNA in biological material at room temperature	4
Decoding Disease: Scott Patterson’s Perspectives on the Power of Biomarkers in Drug Development	4
From Bench to Bedside: Three Imperatives for Making the Digital Laboratory a Reality	4
A domed window chamber for multi-modality optical imaging	4
A Single-wavelength Flow Cytometric Approach using Redox-sensitive Green Fluorescent Protein Probes for Measuring Redox Stress in Live Cells	4
Determination of n-3 Index and Arachidonic Acid/eicosapentaenoic Acid Ratio in Dried Blood Spot by Gas Chromatography	4
Impact of commercial RNA extraction methods on the recovery of human RNA sequence data from archival fixed tissues	4
Rapid Visual Detection of SARS-CoV-2 by Colorimetric Loop-mediated Isothermal Amplification	4
The Earth BioGenome Project: A Success Story for Whole-Genome Sequencing?	4
Correction	4
eDNA Extraction Protocol for Metagenomic Studies in Tropical Soils	4
Instant Pot for Antigen Retrieval: A Simple, Safe and Economical Method for Use in Immunohistochemistry	4
Mito-kaede photoactivation and chase experiment for mitophagy: optimizing flux measurement via fluid exchange system	3
Design Considerations for Point-of-Need Devices Based on Nucleic Acid Amplification for COVID-19 Diagnostics and Beyond	3
Comparison of commercially available DNA and RNA extraction kits for wildlife feces collected from the environment	3
Screening for Androgen Agonists using Autonomously Bioluminescent HEK293 Reporter Cells	3

Metagenomic Nanopore Sequencing Versus Conventional Diagnosis for Identification of the Dieback Pathogens of Mango Trees	3
Comparison and optimization of protocols and whole-genome capture conditions for ancient DNA samples	3
Prediction of High Fecal Propionate-to-Butyrate Ratios Using 16S rRNA-Based Detection of Bacterial Groups with Liquid Array Diagnostics	3
Assay System for Mesocotyl Elongation and Hydrotropism of Maize Primary Root in Response to Low Moisture Gradient	3
Simple, Inexpensive and RNase-Free Purification of Plasmid DNA by Fractional Precipitation with Isopropanol	3
Reliable Budding Pattern Classification of Yeast Cells with Time-Resolved Measurement of Metabolite Production	3
Extracting High-Quality RNA From Formaldehyde-Fixed Naturally Aged Neuromusculoskeletal Tissues	3
A Comparison of Six DNA Extraction Protocols for 16S, ITS and Shotgun Metagenomic Sequencing of Microbial Communities	3
Diagnosis of Genitourinary Tuberculosis by Loop-Mediated Isothermal Amplification Based on SYBR Green I Dye Reaction	3
Developing Virtual and Augmented Reality Applications for Science, Technology, Engineering and Math Education	3
pJoseph2: a family of plasmids as positive controls for bacterial protein expression, transfections, and western blots	3
Endogenous Cell-free DNA in Fetal Bovine Serum Introduces Artifacts to in Vitro cell-free DNA Models	3
An Assay of Human Tyrosine Protein Kinase ABL Activity using an Escherichia Coli Protein Expression System	3
Democratizing Bioinformatics through easily Accessible Software Platforms for Non-Experts in the Field	3
A Method for the Temperature-Controlled Extraction of DNA from Ancient Bones	3
The Determination of Cystatin C in Biological Samples via the Surface Plasmon Resonance Method	3
Overcoming Problems to Produce the Recombinant Protein LipL21 of Leptospira interrogans	3
Targeted Proteomics Analysis of Plasma Proteins Using Recombinant Protein Standards for Addition Only Workflows	3
Rectal swab DNA collection protocol for PCR genotyping in rats	3
A High-throughput Pipeline for DNA/RNA/small RNA Purification from Tissue Samples for Sequencing	2
A Building Block-Based Beam-Break (B 5 ) Locomotor Activity Monitoring System and Its Use in Circadian Biology Research	2
DNA Extraction from Clotted Blood in Genotyping Quality	2
MSCProfiler: A Single Cell Image Processing Workflow to Investigate Mesenchymal Stem Cell Heterogeneity	2
A Compact and Simple Method of Achieving Differential Transgene Expression by Exploiting Translational Readthrough	2
Bioinformaticians: the Hidden Heroes of the COVID-19 Pandemic	2
A Technique for the Masses: Single-Cell Analysis Using Mass Cytometry	2
PS4: A Next-Generation Dataset for Protein Single-Sequence Secondary Structure Prediction	2
‘Gel-Stacks’ gently confine or reversibly immobilize arrays of single DNA molecules for manipulation and study	2
Considerations for Developing Mitochondrial Transplantation Techniques for Individualized Medicine	2
Comparison of DNA Extraction Methods for Samples from Old Blood Collections	2
Education in Microscopy: Taking a Closer Look with Jennifer Waters	2
Tumor Cell Invasion into Matrigel: Optimized Protocol for RNA Extraction	2
A High-Quality Fungal Genome Assembly Resolved from a Sample Accidentally Contaminated by Multiple Taxa	2
Who Owns Your DNA?	2
How is Spatial Transcriptomics Influencing Cancer Research and Diagnostics?	2
Investigation of Circulating DNA Integrity After Blood Collection	2
Three-Dimensional Visualization and Analysis of Dendritic Spines in Human Brain Tissue	2
Enhanced Blastocystis subtyping from stool samples using semi-nested barcode PCR: validation with an NGS-based approach	2
Accurate, Fast and Cost-Effective Simultaneous Detection of Bacterial Meningitis by Qualitative PCR with High-Resolution Melting	2
Is Space the Final Frontier for Mitochondrial Study?	2
A free and user-friendly software protocol for the quantification of microfauna swimming behavior	2
Welcome to the 72nd Volume of BioTechniques	2
Development of a Highly Sensitive Nucleic Acid Amplification-Based Detection for Human Leptospirosis Infection	2
Western Blotting of Native Proteins from Agarose Gels	2
Target-Seq: Single Workflow for Detection of Genome Integration Site, DNA Translocation and Off-Target Events	1
In Vitro Amplification of Whole Large Plasmids Via Transposon-Mediated oriC Insertion	1
Developing advanced organoids: challenges, progress, and outlook	1
Establishment of Loop-Mediated Isothermal Amplification for Brucella Detection Using a Warmer Pad as a Heating Source	1
Gel Purification of gDNA for Next-Generation Sequencing Applications	1
Comparison of Colorimetric, Spectroscopic and Electrochemical Techniques for Quantification of Hydrogen Sulfide	1
Selective Expansion of Target Cells Using the Enrich TROVO Platform	1
Predicting Molecular Docking of Per- and Polyfluoroalkyl Substances to Blood Protein Using Generative Artificial Intelligence Algorithm Diffdock	1
Crystal Violet Staining is a Reliable Alternative to Bicinchoninic Acid Assay-Based Normalization	1
Bioimprinting: Bringing Together 2D and 3D in Dissecting Cancer Biology	1
Assessment of Measurement Accuracy of Amplified Dna Using a Colorimetric Loop-Mediated Isothermal Amplification Assay	1
A single-cell 3D dynamic volume control system for chondrocytes	1
Artificial intelligence: help or hindrance in solving the reproducibility crisis?	1
An Industry Perspective Approach and Control Strategy for Implementation of Ready-to-Use Cells in Bioassays: Survey Outcome And Recommendations	1
Decoding the Mechanisms of Embryo Development with Spatial Biology	1
Welcome to the 76th Issue of BioTechniques	1
Mapping the spread of antibiotic resistance genes in the coastal microbiome	1
A Simplified and Rapid in Situ Hybridization Protocol for Planarians	1
Quantitative Analysis of lncRNA in Formalin-fixed Paraffin-embedded Tissues of Oral Squamous Cell Carcinoma	1
How has CRISPR Transformed Therapeutic Drug Discovery?	1
Effective Strategies for Isolating DNA from Members of Asteraceae with High Concentrations of Secondary Metabolites	1
Importance of a reference ladder in analytical testing of CRISPR HDR donor ssDNA templates	1
Secure and Optimized Detection of PNPLA3 rs738409 Genotype by an Improved PCR–Restriction Fragment Length Polymorphism Method	1
Auxin-Inducible Degron System: An Efficient Protein Degradation Tool to Study Protein Function	1
Automated Epidermal Thickness Quantification of in Vitro Human Skin Equivalents Using Optical Coherence Tomography	1
The Path to Solving the Protein Folding Problem	1
Optimization of Phosphate-Limited Autoinduction Broth for Two-Stage Heterologous Protein Expression in Escherichia Coli	1
Utilizing the biosimulator to analyze the environmental microbiome within the intensive care units of a hospital	1
Agarose Gel Electrophoresis to Assess PCR Product Yield: Comparison with Spectrophotometry, Fluorometry and qPCR	1
I Know What You're Thinking; Can Neuroimaging Truly Reveal Our Innermost Thoughts?	1
Corrigendum	1
Isothermal amplification of long DNA fragments at low temperature by improved strand displacement amplification	1
HIV: The Pursuit of an Elusive Vaccine	1
The Forensic Genomics Toolbox is Expanding	1
The environmental impact of AI in the lab: a double-edged sword?	1
Room Temperature in Scientific Protocols and Experiments Should be Defined: a Reproducibility Issue	1

Microbial Marvels: Could Microbes Resolve Climate Change?	1
Overlap Extension Cloning of PCR Products into a Gateway-compatible Plasmid Vector	1
Peter and the Beanstalk: Tackling the Giant Questions of Soybean Nodulation	1
Single-cell Sequencing: The Technological Revolution Behind a New Wave of Multiomic Studies in Basic and Cancer Research	1
ncRNAseq: Simple Modifications to RNA-Seq Library Preparation Allow Recovery and Analysis of Mid-Sized Non-Coding RNAs	1
Low-cost Modular Systems for Agarose Gel Documentation	1
Adenovirus Vector System: Construction, History and Therapeutic Applications	1
EXtraction of Cucumber Phloem Sap Based on the Capillary–Air Pressure Principle	1
Influence of Resuspension Volume on Dry Sampling Devices Taken for Human Papillomavirus Testing: Implications for Self-Sampling	1
Improved SARS-CoV-2 PCR Detection and Genotyping with Double-Bubble Primers	1
Who wants to live forever? Models shaping the future of aging research	1
Building a baby: Are Stem Cell-Based, Embryo-Like Models the Key to Unlocking the Secrets of Human Development?	1
Uncovering the molecular mechanisms behind Alzheimer’s and Parkinson’s disease through multi-omics: an interview with Bruno A. Benitez	1
High-Throughput Screening for a SARS-CoV-2 Frameshifting Inhibitor Using a Cell-Free Protein Synthesis System	1
Colonoscopy-Based Intramucosal Transplantation of Cancer Cells for Mouse Modeling of Colon Cancer and Lung Metastasis	1
Releasing Secrets Bound to Ancient Remains with Modern DNA Extraction Techniques: An Interview with Elena Essel	1
Advances in LC-MS/MS Usher in the Era of Single-cell Proteomics	1
Mini-Thermal Platform Integrated with Microfluidic Device with On-Site Detection for Real-Time DNA Amplification	1
Psychobiotics: Are we on the Cusp of a Breakthrough?	1
Gibson Assembly Interposition Improves Amplification Efficiency of Long DNA and Multifragment Overlap Extension PCR	1