OOIR: Observatory of International Research

Papers

(The TQCC of Biotechniques is 5. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2020-05-01 to 2024-05-01.)

Article	Citations
PCR past, present and future	169
Enhancing colorimetric loop-mediated isothermal amplification speed and sensitivity with guanidine chloride	134
Improving RNAi efficiency for pest control in crop species	77
Molecularly imprinted polymers in biological applications	68
Optimization of staining with SYTO 9/propidium iodide: interplay, kinetics and impact on Brevibacillus brevis	49
Flow cytometry: past and future	33
Antibody validation for protein expression on tissue slides: a protocol for immunohistochemistry	33
Genetic molecular markers to accelerate genetic gains in crops	29
Development of multiplexed reverse-transcription loop-mediated isothermal amplification for detection of SARS-CoV-2 and influenza viral RNA	29
Cell-free protein synthesis: advances on production process for biopharmaceuticals and immunobiological products	29
A comparison of six DNA extraction protocols for 16S, ITS and shotgun metagenomic sequencing of microbial communities	27
RNA interference approaches for plant disease control	27
Adenovirus vector system: construction, history and therapeutic applications	27
Critical role of three-dimensional tumorsphere size on experimental outcome	26
Phagocytosis assays with different pH-sensitive fluorescent particles and various readouts	25
Contrast-enhanced ultrasound for the assessment of placental development and function	20
Comparison of target enrichment strategies for ancient pathogen DNA	20
Western blotting: a powerful staple in scientific and biomedical research	17
A new approach to epithelial–mesenchymal transition diagnostics in epithelial tumors: double immunofluorescent staining and flow cytometry	17
A comparison of DNA/RNA extraction protocols for high-throughput sequencing of microbial communities	17
Ladder-shape melting temperature isothermal amplification of nucleic acids	17
Real-time loop-mediated isothermal amplification assay for rapid detection of Fusarium circinatum	17
A novel Ca²⁺ indicator for long-term tracking of intracellular calcium flux	15
Agarose gel electrophoresis to assess PCR product yield: comparison with spectrophotometry, fluorometry and qPCR	15
Injection of Evans blue dye to fluorescently label and image intact vasculature	14

Isolation of extracellular vesicles from intestinal tissue in a mouse model of intestinal ischemia/reperfusion injury	14
A clinically validated human capillary blood transcriptome test for global systems biology studies	14
RPA-PCR couple: an approach to expedite plant diagnostics and overcome PCR inhibitors	14
2D and 3Din vitroassays to quantify the invasive behavior of glioblastoma stem cells in response to SDF-1α	13
Protocols for the preparation and characterization of decellularized tissue and organ scaffolds for tissue engineering	13
High-purity DNA extraction from animal tissue using picking in the TRIzol-based method	12
A device for stereotaxic viral delivery into the brains of neonatal mice	12
Protocols for accelerated production and purification of collagen scaffold and atelocollagen from animal tissues	12
Bioprocess intensification: aspirations and achievements	12
Has DeepMind's AlphaFold solved the protein folding problem?	12
Rapid visual detection of SARS-CoV-2 by colorimetric loop-mediated isothermal amplification	12
High-yield skeletal muscle protein recovery from TRIzol after RNA and DNA extraction	11
Discovery of alliin as a putative inhibitor of the main protease of SARS-CoV-2 by molecular docking	11
Western blotting of native proteins from agarose gels	11
Western blotting (immunoblotting): history, theory, uses, protocol and problems	11
Influence of spore morphology on spectrophotometric quantification of Trichoderma inocula	11
A simplified method to produce mRNAs and functional proteins from synthetic double-stranded DNA templates	10
Automated calibration of optoPlate LEDs to reduce light dose variation in optogenetic experiments	10
Comparison of methods for mapping rhizosphere processes in the context of their surrounding root and soil environments	10
Empowering academic labs and scientists to test for COVID-19	10
Evidence-based point-of-care technology development during the COVID-19 pandemic	10
Unbiased automated quantitation of ROS signals in live retinal neurons of Drosophila using Fiji/ImageJ	10
Crystal violet staining is a reliable alternative to bicinchoninic acid assay-based normalization	10
Application of a robust microplate assay to determine induced β-1,3-glucanase and chitinase activity in the cotton plant	9
TADBD: a sensitive and fast method for detection of typologically associated domain boundaries	9
Increased sensitivity using real-time dPCR for detection of SARS-CoV-2	9
Advances in single cell technologies in immunology	9
Optimization of western blotting for the detection of proteins of different molecular weight	9
Analysis of cancer-related mutations in extracellular vesicles RNA by Droplet Digital™ PCR	9
Effect of different cloning strategies in pET-28a on solubility and functionality of a staphylococcal phage endolysin	8
Developing drugs for the ‘undruggable’	8
Efficient multiplexing and variant discrimination in reverse-transcription loop-mediated isothermal amplification with sequence-specific hybridization probes	8
AroCell TK 210 ELISA for determination of TK1 protein: age-related reference ranges and comparison with other TK1 assays	8
Design and characterization of a 3D-printed staggered herringbone mixer	8
Various factors affect lipopolysaccharide sensitization in cell cultures	8
Targeted proteomics analysis of plasma proteins using recombinant protein standards for addition only workflows	8
Metagenomics: preventing future pandemics	7
Nanoadhesive layer to prevent protein absorption in a poly(dimethylsiloxane) microfluidic device	7
Mini-thermal platform integrated with microfluidic device with on-site detection for real-time DNA amplification	7
Detection and differentiation of bacteria by electrical bioimpedance spectroscopy	7
Magnetic tweezers: development and use in single-molecule research	7
Dielectrophoretic characterization of dendritic cell deformability upon maturation	7
CRISPR vs COVID-19: how can gene editing help beat a virus?	7
Intracellular flow cytometry complements RT-qPCR detection of circulating SARS-CoV-2 variants of concern	7
Automated epidermal thickness quantification of in vitro human skin equivalents using optical coherence tomography	7
Effective strategies for isolating DNA from members of Asteraceae with high concentrations of secondary metabolites	7
Diagnosis of genitourinary tuberculosis by loop-mediated isothermal amplification based on SYBR Green I dye reaction	7
Methods for simultaneous and quantitative isolation of mitochondrial DNA, nuclear DNA and RNA from mammalian cells	6
A method for the temperature-controlled extraction of DNA from ancient bones	6
Development of a Droplet Digital™ PCR DNA methylation detection and quantification assay of prenatal tobacco exposure	6

Organoid model of urothelial cancer: establishment and applications for bladder cancer research	6
Cellular considerations for optimizing bone cell culture and remodeling in a lab-on-a-chip platform	6
pET expression vector customized for efficient seamless cloning	6
A clinically validated human saliva metatranscriptomic test for global systems biology studies	6
Seeing the full picture: advances in 3D cell culture microscopy	6
Emulsion PCR made easy	6
A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissue	6
Single-particle chemical force microscopy to characterize virus surface chemistry	6
eDNA extraction protocol for metagenomic studies in tropical soils	6
Recombinant bacterial endotoxin testing: a proven solution	6
A simple approach for effective shearing and reliable concentration measurement of ultra-high-molecular-weight DNA	6
Reverse complement-PCR, an innovative and effective method for multiplexing forensically relevant single nucleotide polymorphism marker systems	6
A fast, nondestructive method for the detection of disease-related lesions and wounded leaves	6
Quantifying sequencing error and effective sequencing depth of liquid biopsy NGS with UMI error correction	5
Application of the Gyrolab microfluidic platform to measure picomolar affinity of a PD-L1-binding Adnectin™ radioligand for positron emission tomography	5
Advances of multiplex ligation-dependent probe amplification technology in molecular diagnostics	5
MILKSHAKE: novel validation method for antibodies to post-translationally modified targets by surrogate Western blot	5
Comparison of DNA extraction methods for samples from old blood collections	5
Isothermal amplification using sequence-specific fluorescence detection of SARS coronavirus 2 and variants in nasal swabs	5
Improved third-generation lentiviral packaging with pLKO.1C vectors	5
Sociometric wearable devices for studying human behavior in corporate and healthcare workplaces	5
Optimization of phosphate-limited autoinduction broth for two-stage heterologous protein expression in Escherichia coli	5
A simple serum depletion method for proteomics analysis	5
Generation of genetically tailored porcine liver cancer cells by CRISPR/Cas9 editing	5
SUMOylation of the chromodomain factor MRG-1 inC. elegansaffects chromatin-regulatory dynamics	5
tANCHOR: a novel mammalian cell surface peptide display system	5