Reproduction in Domestic Animals

Article	Citations
Production of sexed bovine embryos in vitro can be improved by selection of sperm treatment and co‐culture system	31
Issue Information	25
Measurement of membrane integrity in canine spermatozoa using a fluorescent computer‐assisted spermatozoal quantification method after SYBR‐14/PI staining compared with manual counting after CFDA/PI s	24
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Author Index	23
Posters	22
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Workshops	17
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Birth weight and stillbirth genetic parameter estimation in a river buffalo simulated population	16
Issue Information	15
Peroxisome proliferator‐activated receptor expression in the canine endometrium with cystic endometrial hyperplasia–pyometra complex	15
Comparison of ultrasonography, progesterone assay, ballottement, and non‐return‐to‐heat with digital rectal palpation technique for early pregnancy diagnosis in Red Sokoto goat	14
Regulation of proteolysis in bovine cumulus cells with possible inclusion of proton pump activators	13
Influence of endometritis on the follicular dynamics, recovery, quality, gene expression, nuclear maturation and in‐vitro developmental competence of oocytes in Sahiwal cattle	13
Potential role for human menopausal gonadotropin in ovine superovulatory regimens during the breeding season	12
Clinical Cases Oral	12
Global proteomic analysis of preimplantational ovine embryos produced in vitro	12
Chia (Salvia hispanica L.) can directly suppress basic ovarian cell functions in two farm animal species and protect ovarian cells from the proliferation‐stimulating influence of xylene	12
Issue Information	12
Evaluation of carboxylated poly‐l‐lysine for cryopreservation of Labeo rohita sperm	12
Oxidative response of sheep to transcervical applications	12
Issue Information	12
Issue Information	12
Analysis of PPP1R11 expression in granulosa cells during developmental follicles of yak and its effects on cell function	11

The post‐ovulatory rise in progesterone is lower and the persistence of oestrous behaviour longer during the first compared with the second cycle of the breeding season in mares	11
Chromatin openness of donor cells is directly correlated with the in vitro developmental capabilities of cloned buffalo embryos	11
Prostatic fluid composition and semen quality in dogs with benign prostatic hyperplasia undergoing treatment with osaterone acetate	11
Evaluating the use of piezo manipulator, laser or their combination for blastocoel cavity puncture to improve cryopreservation outcomes of large equine embryos	10
55th Annual Conference of Physiology and Pathology of Reproduction and 47th Joint Conference of Veterinary and Human Reproductive Medicine	10
Integrated testis transcriptome and whole genome analysis of sexual maturity in Large White and Tongcheng pigs	10
Issue Information	10
Flow cytometric evaluation of canine follicular cell apoptosis during the oestrous cycle	10
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Association between novel variants in BMPR1B gene and litter size in Mongolia and Ujimqin sheep breeds	10
Determination of expression patterns of miR‐26a, and preimplantation factor levels for early pregnancy detection in nulliparous and multiparous cows	9
The effect of alfalfa supplementation during the peripartum period on udder oedema and fertility in dairy sheep	9
Issue Information	9
Contraceptive effect of a gonadotropin‐releasing hormone vaccine on captive male brown bears (Ursus arctos)	9
Serum anti‐Müllerian hormone around the time of ovulation simulated by exogenous hormones in clouded leopards (Neofelis nebulosa)	9
Impact of Periconceptional and Gestational Vitamin D3 Restriction on Fetal Folliculogenesis and Anti‐Mullerian Hormone Secretion Using Sheep as a Model	9
Zinc oxide nanoparticles functionalized with curcumin supplementation during in vitro embryo culture impaired in a concentration‐dependent‐manner blastocyst production in cattle	9
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Pathogenesis of the crosstalk between reproductive function and stress in animals—part 1: Hypothalamo–pituitary–adrenal axis, sympatho‐adrenomedullary system and kisspeptin	8
Issue Information	8
Workshops	8
Effect of ascorbic acid on metabolic status, lipid peroxidation, antioxidant activity and quality of frozen Indian red jungle fowl (Gallus gallus murghi) semen	8
Effects of niacin supplementation during in vitro culture on the developmental competence of porcine embryos	8
Mammary carcinoma: Comparative oncology between small animals and humans—New therapeutic tools	8
Rotation of liquid‐preserved artificial insemination doses on roller benches affects sperm quality during storage in stallions	8
Histological study of Bactrian camel cryptorchidism and expression of immunoglobulin λ light chain in the testicular and epididymis of cryptorchid Bactrian camel	8
Cryoprotectant role of exopolysaccharide ID1 in the vitrification/in‐straw warming of in vitro‐produced bovine embryos	8
Recipients’ pregnancy rate was affected by season but not by the temperature‐humidity index (THI) in an equine commercial ET programme in Southern Europe	8
Cyclodextrin‐Cholesterol Alone or in Combination With Cyclodextrin‐Vitamin E Improves Bull Sperm Cryopreservation in a Soybean Lecithin Extender	7
Effect of lowering blood cortisol level along with progesterone priming on intensifying the secondary symptoms of oestrus in Murrah buffaloes during the hot summer months	7
TLR‐2 and TLR‐4 mRNA expression in different grades of histopathological lesions of equine endometrium from follicular phase	7
Mobility of Japanese quail spermatozoa and its relationship to egg fertility	7
Why choose the rabbit to work in reproductive technology?	7
A Novel Intravaginal Contraceptive Plug for Cats: A Preliminary Biocompatibility Assessment on Haematology and Vaginal Swab	7
Enhancing felid conservation: Exploring the impact of in vitro culture media on domestic cat blastocyst production	7
Plasma cell‐free DNA concentration increases during luteolysis in beef cows	7
The growth factors amphiregulin and epiregulin are novel stimulators of feline ovarian granulosa cell functions	7
Issue Information	7
Assessment of the stallion sperm acrosome in two different extenders: Spermac stain in comparison with PNA/PSA/PI triple‐staining	7
Damage to Mitochondria During the Cryopreservation, Causing ROS Leakage, Leading to Oxidative Stress and Decreased Quality of Ram Sperm	7
Advanced assisted reproduction technologies in endangered mammalian species	7
Impact of papain on the treatment of raw diluted dromedary semen	7
Comparative productive performance of cows born through embryo transfer, artificial insemination and natural mating in dairy and dual‐purpose herds raised in tropical conditions	6
Issue Information	6
Heparin‐induced and caffeine or ouabain supplemented capacitation of frozen‐thawed yak (Bos grunniens) spermatozoa	6
Low oxygen concentration improves yak oocyte maturation and inhibits apoptosis through HIF‐1 and VEGF	6
Imaging flow cytometry to characterize the relationship between abnormal sperm morphologies and reactive oxygen species in stallion sperm	6
Transcriptome profiles of pre‐pubertal and adult in vitro matured ovine oocytes obtained from FSH‐stimulated animals	6
Issue Information	6

Association between serum mineral levels and reproductive performance in primiparous dairy cows during the peripartum period	6
Vascular endothelial growth factor (VEGF) expression in histological endometritis in the bitch: An immunohistochemical study	6
Proliferation and apoptosis dynamics of the normal canine mammary gland during the oestrous cycle evaluated by Ki‐67 and Caspase‐3 indexes	6
Influence of the coincubation of post‐thawed bull semen with elevated β‐hydroxybutyrate concentrations on sperm characteristics	6
Metabolomic profile of seminal plasma from Guzerá bulls (Bos indicus) with contrasting sperm freezability phenotypes	6
The use of sodium caseinate in the freezing of sheep semen	6
Cow and management factors and their association with estimates of uterine size and position at the time of insemination of dairy cattle	6
Expression dynamics of adipokines during induced ovulation in the bovine follicles and early corpus luteum	6
Morphometric changes on the vulva from proestrus to oestrus of nulliparous and multiparous HYPERPROLIFIC sows	6
The use of oxytocin to cause cervical dilation for transcervical insemination in nulliparous goats: Improving pregnancy and kidding rates	6
Antimicrobial susceptibility and production of virulence factors by bacteria recovered from bitches with pyometra	6
Melatonin improves the viability and ultrastructure of bovine oocyte‐granulosa complexes of in vitro cultured early antral follicles	6
Activin A attenuates apoptosis of granulosa cells in atretic follicles through ERβ‐induced autophagy	5
Effect of L‐citrulline supplementation on sperm characteristics and hormonal and antioxidant levels in blood and seminal plasma of rams	5
Tubular ectasia of the rete testis in an Angus bull	5
Effects of supplementation of grazing Nellore cows with β‐carotene and vitamins A + D3 + E + biotin on follicle diameter, oestrus, establishment of pregnancy, and foetal morphometry	5
Retrospective analysis of commercial heterospermic and homospermic cooled boar semen: Effect of the season, sample type and shipping temperature on sperm quality	5
Predictive Indicators of Cryotolerance and Fertility in Bovine Sperm: Evaluating Fresh Semen Quality to Improve AI Outcomes With Frozen–Thawed Sperm	5
Evaluation of clinical and subclinical endometritis impacts on the reproductive performance and milk production of dairy cows in Brazilian herds	5
Platelet‐rich plasma in animal reproductive medicine: Prospective and applications	5
Uterine tone: A neglected criterion for the selection of bovine embryo transfer recipients	5
Causes of pregnancy arrest in the canine species	5
Evaluation of blood minerals and oxidative stress changing pattern in prepartum and postpartum Achai and Holstein Friesian dairy cows	5
Gene expression pattern and molecular mechanisms involved in Shal and Sangsari sheep fertility using RNA‐Seq	5
Identification of internal reference genes for porcine immature Sertoli cells under heat stress	5
Anti‐Müllerian hormone as a promising novel biomarker for litter size in Romanov sheep	5
Prevalence and antimicrobial resistance trends among vaginal bacteria isolates from pregnant bitches	5
Interaction of blood calcium with milk yield, energy metabolites, subclinical mastitis, and reproductive performance in crossbred dairy cows	5
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Invited review: Bovine neonatal morbidity and mortality—Causes, risk factors, incidences, sequelae and prevention	5
Milk fatty acids as indicators of delayed commencement of luteal activity in dairy cows in early lactation	5
Aucubin ameliorates the LPS‐induced inflammatory response in bovine endometrial epithelial cells by inhibiting NF‐κB and activating the Keap1/Nrf2 signalling pathway	5
Influence of different warming temperatures on the vitrified testicular fragments from pre‐pubertal cat	5
Immune defences of the mammary gland in dairy ruminants	5
The addition of crocin in the freezing medium extender improves post‐thaw semen quality	5
Effects ofOriganum majoranaessential oil and antibiotics on the quality of frozen thawed Beni Arouss buck semen	5
Temporal variation and risk factors associated with reproductive disorders in Hardhenu (Bos taurus × Bos indicus) dairy cattle	5
Issue Information	5
Metabolic signature of spent culture media shows lipid metabolism as a determinant of pregnancy outcomes	5
Reprotoxic effects of fenpropimorph on the fertilizing potential of AI boars: A case study	5
CRISPR‐Mediated SRY Gene Mutation Increases the Expression of Female Lineage‐Specific Gene in Pre‐Implantation Buffalo Embryo	5
Recent approaches in the use of antioxidants and proteomic modifications in ram semen preservation	5
Expression of reproductive‐related genes and changes in oocyte maturation of goldfish broodstock (Carassius auratus) following injection of different exogenous kisspeptins	5
Exploring oxidative stress, immunological and metabolic biomarkers in dairy cows with postpartum pyometra	5
Assessment of antibiotic use patterns in bovine mastitis treatment in the dairy sector in Serbia	5
Fertilization with follicular fluid reduces HSP70 and BAX expression on bovine in vitro embryos	5
Estimation of sperm concentration limits to produce intrauterine insemination doses in swine	5
Issue Information	5
Comparative transcriptome analysis identifies important maternal molecules and associated biological pathways for pig and human mature oocytes	4
Renal biomarkers, clinical parameters, and renal Doppler velocimetry in bitches with cystic endometrial hyperplasia–pyometra complex	4
AUTHOR INDEX	4
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Abstract	4
Welcome Note to the Participants of the 58th Annual Conference of Physiology and Pathology of Reproduction and 50th Joint Conference of Veterinary and Human Reproductive Medicine (February 26–28, 2025	4
Effect of addition of ascorbate, dithiothreitol or a caspase‐3 inhibitor to cryopreservation medium on post‐thaw survival of bovine embryos produced in vitro	4
Seasonality analysis on cryopreserved doses from the autochthonous cattle breeds Asturiana de la Montaña and Asturiana de los Valles	4
Sperm Motility Evaluation in Stallion Fresh, Cooled and Frozen Semen Using a Portable Computer‐Assisted Sperm Analysis System	4
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Testicular haemodynamics, plasma testosterone and oestradiol concentrations, and serum nitric oxide levels in the Egyptian buffalo bull after a single administration of human chorionic gonadotropin	4
Aetiological analysis and diagnosis of reproductive disorders in male dromedary camels	4
Role of aquaporin 3 in reproductive performance of dairy goats after repeated estrus synchronization stimulation	4
Effects of Electroporation Timing and Cumulus Cell Attachment on In Vitro Development and Genome Editing of Porcine Embryos	4
Oral communications	4
Issue Information	4
Prolonging the time of semen deposition increases the pregnancy rates of ewes subjected to fixed time cervical insemination during the breeding season	4
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Can roscovitine and trichostatin A be alternatives to standard protocols for cell cycle synchronization of ovine adult and foetal fibroblast cells?	4
Metabolomic profiling of Dezhou donkey seminal plasma related to freezability	4
Efficiency of three boar sperm enrichment techniques	4
Methods for Evaluating Testicular Function in Domestic Cats	4
Two successful embryo transfers of mini‐donkey embryos in Brazilian Northeastern jennies using an alternative method: Case report	4
Donkey semen cryopreservation: Alternatives with permeable, non‐permeable cryoprotectants and seminal plasma	4
Serum supplementation during in vitro fertilization of sheep oocytes influences blastocyst quality through the differential abundance of mRNA transcripts	4
Induction of ovulation using repeated doses of sulpiride, a dopamine antagonist, in ewe lambs	4
Study on the expression patterns and function of JUNO and CD9 in bovine oocytes during in vitro maturation	4
Kisspeptin stimulates oestradiol biosynthesis by upregulating steroidogenic transcripts and proliferation markers in the bubaline granulosa cells in vitro	4
Embryo production and transplantation in non‐breeding season of meat sheep breeds by stimulating superovulation with different follicle‐stimulating hormone preparations	4
Bovine somatotropin increases the pregnancy rate in fixed‐time artificial insemination in beef cattle	4
Supplementation of media with gamma‐oryzanol as a novel antioxidant to overcome redox imbalance during bovine oocyte maturation in vitro	4
Media viscosity affects post‐thaw ram sperm rheotactic behaviour	4

PCR‐Based Amplification of Amelogenin Gene for Ovine Sex Determination Using Primers of Different GC Percentage	4
Recent advances in donkey sperm vitrification	4
Ovarian Dynamics and Pathological Conditions in Camelids	4
Investigation of the Effect of Asprosin Level on Fertility Success and Oxidative Stress in Simmental Cows	4
Maladaptation to the transition period and consequences on fertility of dairy cows	4
Expression of oxytocin receptors and oxytocin assisted electroejaculation in the domestic cat (Felis catus)	4
Estimation of maternal and additive effect on reproduction and productive traits in Hardhenu cattle	3
Chlorogenic acid improves the development of porcine parthenogenetic embryos by regulating oxidative stress and ameliorating mitochondrial function	3
The effect of exogenous melatonin administration before superovulation on embryo yield in Assaf ewes	3
Adjusting ram semen preservation: Exploring the impact of oxygen exposure during liquid storage	3
Effect of the rob(1;29) translocation on the fertility of beef cattle reared under extensive conditions: A 30‐year retrospective study	3
RBP4 regulates androgen receptor expression and steroid synthesis in Sertoli cells from Bactrian camels	3
Bovine congenital defects recorded by veterinary practitioners	3
Secretory leucocyte protease inhibitor (SLPI) as an adjunct prognostic biomarker for canine pyometra	3
Issue Information	3
Effect of three schemes of ovum pick‐up on the follicular dynamics, gene expression, and in‐vitro developmental competence of oocytes in Sahiwal cattle	3
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Estimation of genetic parameters and genetic change of first parity reproductive traits in Alpine × Beetal goats	3
Mechanisms by which mastitis affects reproduction in dairy cow: A review	3
Induction of reproductive activity and egg production by gonadotropin‐releasing hormone in non‐laying hens	3
Effect of exogenous oestrogen treatment frequency on induction of artificial lactation in pseudopregnant sows	3
Morphology and morphometry of sperm in Kurdish stallions, a local breed from western Iran	3
Comparison of blood ΒHBA measurement devices for diagnosis of subclinical pregnancy toxaemia in sheep: A field study	3
Evaluation of parity and its interaction effect with season of calving, period of calving on production and reproduction traits in Hardhenu (Bos taurus × Bos indicus) dairy cattle	3
Cloprostenol effect with and without male stimulation on oestrus synchronization in Peru guinea pigs	3
Successful short‐term sperm cryopreservation in brown‐marbled grouper (Epinephelus fuscoguttatus) with the utility of ultra‐freezer (−80°C)	3
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Editorial	3
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Region‐specific gene expression profile in the epididymis of high‐ and low‐fertile dromedary camels	3
Pregnancy rate per cycle is heritable and reduces with cycle in naturally mated tropically adapted beef cows	3
Melatonin affects red deer spermatozoa motility and physiology in capacitating and non‐capacitating conditions	3
Strategies to improve the success of fixed‐time artificial insemination in the ewe	3
Calving date as a potential predictor for the probability of approval in the first breeding soundness evaluation of Nellore bulls	3
Lipid availability during in vitro maturation alters oocyte lipid content and blastocyst development and metabolism	3
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Extended lactations in dairy cows and the effects on fertility and production	3
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Effect of calving to timed artificial insemination interval on fertility of beef cows	3
Anti‐Müllerian hormone as an endocrine biomarker of reproductive longevity and assessment of single nucleotide polymorphisms in AMH gene of Bos indicus breeds of cattle	3
Issue Information	3
Association of genotypes at rs438228855 in bovine SLC35A3 receptor gene of Pakistani cattle with the susceptibility to develop complex vertebral malformation	3
Single‐cell RNA sequencing reveals blastomere heterogeneity of 2‐cell embryos in pigs	3
Macrophages in bovine term placenta: An ultrastructural and molecular study	3
Cholecystokinin‐mediated effect of insulin pathway on the steroidogenic activity of follicular granulosa cells in Camelus bactrianus: In vitro study	3
Administration of carbetocin—a long‐acting oxytocin analogue—before sperm collection by transrectal ultrasound‐guided massage of the accessory sex glands in bucks (Capra hircus) and ibexes (	3
Dynamics of transcription is affected by oxygen tension and developmental speed during in vitro production of bovine embryos	3
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Reproductive performance and progeny sex ratio of female Chukar (Alectoris chukar) breeder partridges reared on restricted feeding regimens	3
Orexin system in buffalo ovarian follicles and effect of orexin on oestradiol production	3
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Effects of cryopreservation on the mitochondrial bioenergetics of bovine sperm	3
Oxytocin increases pregnancy rates after fixed time artificial insemination in Kazak ewes	3
Proteomic profile in plasma as a biomarker of pregnancy in cows	3
Young Scientist Competition	3
Issue Information	3
Correction to ‘Effects of hypertonic dextrose and paraffin solution as non‐antibiotic treatments of clinical endometritis on reproductive performance of high‐producing dairy cows’	3
Live cells are not affected by dead sperm in liquid boar semen: New insights based on a thermo‐resistance test	3
Effect of ovarian follicular wave pattern and endocrine characteristics on pregnancy outcome in cows	3
Effect of pregnancy length on the early development of Bengal kittens	3
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Issue Information	3
Association between levels of insulin‐like growth factor‐1 in serum and seminal plasma with fresh and frozen‐thawed semen characteristics in Beetal bucks	3
The effect of priming and duration of oestradiol benzoate treatment before progesterone administration on embryo development and survival in anestrous recipient mares	3
Differential expression and functional prediction of mRNA in the ovaries of Hanper sheep of high and low fecundity	3
Transvaginal ultrasound‐guided oocyte retrieval in cattle: State‐of‐the‐art and its impact on the in vitro fertilization embryo production outcome	3
Effects of different extenders on epigenetic patterns and functional parameters of bull sperm during cryopreservation process	3
Epsilon poly‐lysine in buffalo semen extender: A step towards reducing the development of antibiotic resistance	3
Oral supplementation with Scabiosa artropurperea L. aqueous extract improves the in vivo sexual behaviour, sperm quality and androgen level in rams	3
Seasonal changes in testis size, testosterone levels and sperm production quality in meat rams	3
High percentage of midpiece defects in Brangus bull sperm with no reduction in sperm kinematics	3
Effects of extender filtration and egg yolk concentration on canine semen cryopreservation	3
Equilibration time with cryoprotectants, but not melatonin supplementation during in vitro maturation, affects viability and metaphase plate morphology of vitrified porcine mature oocytes	3
Interspecies cell fusion between mouse embryonic stem cell and porcine pluripotent cell	3
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Prevalence and Morphological Appearance of Sperm Nuclear Defects Using Feulgen Reaction in Beef Bulls	2
Clinical endometritis with Trueperella pyogenes reduces reproductive performance and milk production in dairy cows	2
Different protocols in treatment of placental retention in dairy cows and their influences on reproductive performance	2
Cryopreservation of local black rabbit spermatozoa in the Mekong delta region of Vietnam: Evaluating cryoprotectant effectiveness	2
G protein‐coupled estrogen receptor 1 inhibits the epithelial–mesenchymal transition of goat mammary epithelial cells via NF‐κB signalling pathway	2
A comparative transcriptome and proteomics study of post‐partum ovarian cycle arrest in yaks (Bos grunniens)	2
Cryopreservation‐Induced Changes in Protamine Levels and DNA Fragmentation in Alpaca Spermatozoa	2
Nongenomic Progesterone Receptors Mediated Bull Sperm Capacitation and Acrosome Reaction Are cAMP‐PKA‐Calcium Channel Dependent	2
Validation of pregnancy associated glycoproteins‐basedELISAkits to determine early pregnancy status in lactatingNili‐Ravibuffaloes	2
Latest update on predictive indicators, risk factors and ‘Omic’ technologies research of retained placenta in dairy cattle – A review	2
Issue Information	2
Content of tryptophan and kynurenines in serum and milk of dairy cows with mastitis caused by Streptococcus spp.	2
Evaluation of DNA fragmentation in dog sperm using the sperm chromatin dispersion test	2
New Insights in Bovine Follicular Cysts	2
Genetic group and swim‐up processing affect SYBR Green real‐time PCR‐based porcine semen sex ratio in Landrace and its crossbred boars	2
Exogenous L‐arginine administration improves uterine vascular perfusion, uteroplacental thickness, steroid concentrations and nitric oxide levels in pregnant buffaloes under subtropical conditions	2
Effects of daily propylene glycol drenching during the Ovsynch protocol on fertility and metabolic parameters in lactating dairy cows	2
Higher quality rather than superior quantity of oocytes determine the amount of fertilizable oocytes in two outbred Dummerstorf high‐fertility mouse lines	2
Progesterone supplementation to improve fertility of selected subgroups of lactating cows during the summer and fall	2
Age‐related changes in testicular morphometry and function in Egyptian donkeys	2