Current Opinion in Biotechnology

Article	Citations
Editorial Board	196
Editorial Board	121
Corrigendum to “Methylotrophs: from C1 compounds to food” [Curr Opin Biotechnol 75 (2022) 102685]	110
Anaerobic microbial methanol utilization as a one-carbon feedstock	104
Bacterial degradation of emerging aromatic pollutants and integrated strategies for sustainable healthy agro-ecosystem	101
Tumor lipid metabolism: a mechanistic link between diet and cancer progression	94
Spatial enzyme assembly for microbial pharmaceutical synthesis	90
Cytochrome P450-mediated structural diversification of diketopiperazine alkaloids	86
Advances in synthetic microbial consortia for improved production of biochemicals and biofuels	80
Creating value from purple phototrophic bacteria via single-cell protein production	79
The osteo-angiogenic signaling crosstalk for bone regeneration: harmony out of complexity	76
Designing the future of food fats: precision fermentation of Yarrowia lipolytica for tailored lipid production	76
Synthetic biology advances towards a bio-based society in the era of artificial intelligence	75
How much could improving photosynthesis increase crop yields? A call for systems-level perspectives to guide engineering strategies	71
Unlocking the biotechnological potential of traditional fermented food microbiomes	68
Recent progress in the synthesis of advanced biofuel and bioproducts	66
The journey of a lifetime — development of Pfizer’s COVID-19 vaccine	65
Editorial Board	65
Cell-free technologies for biopharmaceutical research and production	65
Editorial Board	64
Innovations and trends in antibody repertoire analysis	64
Molecular methods for high-throughput, multiplexed, and automated genome editing in prokaryotes and eukaryotes	61
Engineered living bioassemblies for biomedical and functional material applications	61
Coupling chemistry and biology for the synthesis of advanced bioproducts	61
A molecular assessment of the practical potential of DNA-based computation	59

Engineered T cells and macrophages: two arms to seize solid tumors	59
Metabolic engineering of microorganisms for the production of fatty acid-derived biofuels	59
Microfluidics meets cell-free systems: from molecular engineering to synthetic cells	58
Deep learning in regulatory genomics: from identification to design	58
Emerging technologies for genetic control systems in cellular therapies	57
Lighting the way: recent developments and applications in molecular optogenetics	56
Strategies and challenges for synthetic apomixis	55
Recent advances, opportunities and challenges in cybergenetic identification and control of biomolecular networks	55
Expanding the catalytic repertoire of C–C bond–forming pyridoxal 5′-phosphate-dependent enzymes for noncanonical amino acid formation	53
Mass spectrometry imaging methods for visualizing tumor heterogeneity	52
Single-cell analysis opens a goldmine for plant functional studies	52
Perspectives for self-driving labs in synthetic biology	52
Photoprotective-based strategies to enhance crop yield under fluctuating light conditions	51
Next-generation brewing yeasts for non-alcoholic beers	51
Engineering of the genetic code	50
Biocatalytic cascade transformations for the synthesis of C-nucleosides and N-nucleoside analogs	48
Computational tools and resources for designing new pathways to small molecules	47
Deciphering the guanidine code: from core metabolism to biosynthesis of its derivatives	46
Emerging trends in production of plant natural products and new-to-nature biopharmaceuticals in yeast	46
Strategies for engineering domesticated and undomesticated human microbes	45
2-hydroxyglutarate rides the cancer-immunity cycle	45
Advancing in vivo reprogramming with synthetic biology	44
Building trust in automated experimentation: uncertainty quantification in the era of high-throughput biolabs	44
Multifaceted metabolic role of infections in the tumor microenvironment	44
Matching diverse feedstocks to conversion processes for the future bioeconomy	44
Advances in metabolic engineering for the production of sustainable aviation fuels	43
Editorial Board	43
Tissue engineering in the agri-food industry: current status, socio-economic overview and regulatory compliance	43
Plant synthetic biology as a tool to help eliminate hidden hunger	43
Harnessing the potential of microbial methane utilization for chasing sustainability	42
Advances in multiplex precision genome editing in eukaryotic and prokaryotic systems	42
Reducing crop losses by gene-editing control of organ developmental physiology	41
Plant microbiome regulation for sustainable agriculture	41
The potential of amoeba-based processes for natural product syntheses	41
Editorial overview: Pioneering innovations in food biotechnology	41
Corrigendum to: “Design, construction, and application of noncanonical redox cofactor infrastructures” [Curr Opin Biotechnol 84 (2023) 103019]	41
PRRs and NLRs sans frontières: advances and challenges in transfer of immune receptors between plant species	40
Single-cell omics analysis with genome-scale metabolic modeling	40
Synthetic biology approaches for dynamic CHO cell engineering	40
Molecules to medicine: advances in metabolomics for natural product drug discovery	37
Sourcing DNA parts for synthetic biology applications in plants	36
Pathway engineering for the biosynthesis of psychedelics	36
Synthetic apomixis: the beginning of a new era	35
Low-cost, point-of-care biomarker quantification	35
Impact of anthropogenic contamination on glacier surface biota	35
Metabolic and cellular engineering for the production of natural products	35
Biochemical production with microbial bioelectrochemical systems	34
What’s new in single-cell proteomics	34
Editorial overview: Nanobiotechnology for immunoengineering	34
Editorial Board	34

Matchmaking at the cell surface using bispecifics to put cells on their best behavior	34
Understanding atmospheric intercontinental dispersal of harmful microorganisms	34
Engineered methane biocatalysis: strategies to assimilate methane for chemical production	34
Operation at thermophilic temperatures: an underestimated asset for gas fermentation processes	34
Strategies and opportunities for engineering antifungal peptides for therapeutic applications	33
Potential use of microbial engineering in single-cell protein production	33
Synthetic fuels: what are they and where do they come from?	33
The aging tumor metabolic microenvironment	32
Targeting ripening regulators to develop fruit with high quality and extended shelf life	32
Mind the gap: the challenges and opportunities for genomics-driven harnessing of plant metabolic diversity for therapeutic applications	32
Programmable synthetic biology tools for developing microbial cell factories	32
Understanding and harnessing the complexity of interspecies interactions in acetogenic mixotrophic co-cultures	32
The food microbiome: an evolutionary architect, a modern healer, and a future shield	32
Extracellular vesicles as therapeutics for inflammation and infection	32
Bridging the gender gap in autoimmunity with T-cell–targeted biomaterials	31
Biotechnology approaches for natural product discovery, engineering, and production based on Burkholderia bacteria	31
Engineering bacterial warriors: harnessing microbes to modulate animal physiology	31
Editorial Board	31
Next-generation biological vector platforms for in vivo delivery of genome editing agents	31
Challenges and future perspectives for high-throughput chimeric antigen receptor T cell discovery	31
Editorial Board	31
Beyond vancomycin: recent advances in the modification, reengineering, production and discovery of improved glycopeptide antibiotics to tackle multidrug-resistant bacteria	30
Strategies for uncovering stable isotope tracing patterns between cell populations	30
Optimizing the value of bioinks and robotics to advance in vivo bioprinting	30
Non-agricultural feedstocks for next-generation biomanufacturing with yeasts	29
Recent advances in microbial lignin degradation and upcycling of lignin-derived aromatic compounds	29
Can digital twin efforts shape microorganism-based alternative food?	29
De novo domestication: retrace the history of agriculture to design future crops	29
Engineering whole-cell catalysts to use plastic waste as a feedstock	29
Advances in engineering and optimization of transcription factor-based biosensors for plug-and-play small molecule detection	28
In situ characterization of the tumor microenvironment	28
Recent innovations in cannabinoid chemistry, biology, and biosynthesis	28
Editorial Board	28
Microbiological food safety considerations in shelf-life extension of fresh fruits and vegetables	28
Editorial overview: Tissue, cell, and pathway engineering	28
Machine learning for predicting phenotype from genotype and environment	28
Quantitative and analytical tools to analyze the spatiotemporal population dynamics of microbial consortia	27
Achieving net zero CO2 emission in the biobased production of reduced platform chemicals using defined co-feeding of methanol	27
Genetic and process engineering for polyhydroxyalkanoate production from pre- and post-consumer food waste	27
Engineering next-generation chimeric antigen receptor-T cells: recent breakthroughs and remaining challenges in design and screening of novel chimeric antigen receptor variants	27
Senescent cells in tissue engineering	27
Two in one: the emerging concept of bifunctional antibodies	27
Biologics-based degraders — an expanding toolkit for targeted-protein degradation	27
Clustered regularly interspaced short palindromic repeats tools for plant metabolic engineering: achievements and perspectives	27
Upcycling food processing byproducts via advanced yeast fermentation	27
Discovery of de novo peptides by mRNA and RaPID displays with post-translational modification methods	26
Progress and challenges in the translation of cancer nanomedicines	26
In vitro enzyme-mediated conversion of methane and methanol toward value-added chemicals	26
Recent trends in the modification of polyphenolic compounds using hydroxylation and glycosylation	26
Design of DNA strand displacement reactions	26
An integrated eco-evolutionary framework to predict population-level responses of climate-sensitive pathogens	26
Advances in engineering substrate scope of Pseudomonas cell factories	26
Sustainability in food-waste reduction biotechnology: a critical review	26
Decoding dissolved information: environmental DNA sequencing at global scale to monitor a changing ocean	26
Biosynthesis of advanced biofuels in microbial cell factories	26
Searching for the optimal microbial factory: high-throughput biosensors and analytical techniques for screening small molecules	25
Integrating bioprinting and optogenetic technologies for precision plant tissue engineering	25
Advances in artificial biosynthesis of bioactive plant natural products	25
Path to zero emission of nitrous oxide in sewage treatment: is nitrification controllable or avoidable?	25
Engineering plant–microbe communication for plant nutrient use efficiency	25
Proteomics insights into the fungal-mediated bioremediation of environmental contaminants	25
Recent advances in microbial production of rare sugars and steviols	25
Minimum viable products in precision fermentation: defining translational milestones for food biotechnology	25
Mycotoxin risks are lower in biotech corn	25
Understanding methanol metabolism through systems biology: advances and future perspectives	25
Carbon recycling with synthetic CO2 fixation pathways	25
Drugging the microbiome and bacterial live biotherapeutic consortium production	24
Recent advances in microbial synthesis of polyphenols	24
Machine learning applications to improve flavor and nutritional content of horticultural crops through breeding and genetics	24
Engineering organoids as cerebral disease models	24
Approaches for a more microbiologically and chemically safe dried fruit supply chain	24
Advances in engineering algae for biofuel production	24
Microbial C1 assimilation pathways for chemical synthesis: from native metabolism to synthetic design	24
Engineering bacteria for cancer immunotherapy	24
Anaerobic microbial core for municipal wastewater treatment — the sustainable platform for resource recovery	24
Pleiotropy, a feature or a bug? Toward co-ordinating plant growth, development, and environmental responses through engineering plant hormone signaling	24
Eubacterium limosum as a biocatalyst for sustainable methanol bioconversion	24
Shaping Aspergillus niger into a next-generation cell factory for L-malic acid	24
Building mutational bridges between carbohydrate-active enzymes	24
Architectures of emerging biofoundry platforms for synthetic biology	24

Deep learning and generative artificial intelligence methods in enzyme and cell engineering	23
Designed dietary fibers: engineering carbohydrate structure for precision modulation of the human gut microbiome	23
Getting better all the time — recent progress in the development of CRISPR/Cas-based tools for plant genome engineering	23
Combining enzyme and metabolic engineering for microbial supply of therapeutic phytochemicals	22
Quantifying nanoparticle delivery: challenges, tools, and advances	22
Reprogramming immunity: TAL effector-informed genome editing in rice and other crops	22
Cell-free biosensors: where have we been and where do we need to go?	21
Engineering microbial consortia with rationally designed cellular interactions	21
A guide to designing cell-surface receptors in plants	21
Filamentous fungi for future functional food and feed	21
Engineering red cells across scales: Red Blood Cells (RBCs) and RBC-derived extracellular vesicles	21
Continuous biomanufacturing with microbes — upstream progresses and challenges	21
Industrial potential of the enzymatic synthesis of nucleoside analogs: existing challenges and perspectives	21
Editorial overview: Phosphate Special Issue	21
Pathway engineering for sustainable biomanufacturing: integrating AI, systems biology, enzyme engineering, and dynamic control	21
Cell-free enzyme cascades — application and transition from development to industrial implementation	21
Biomolecular feedback controllers: from theory to applications	21
Machine learning in wastewater: opportunities and challenges — “not everything is a nail!”	21
Recent advances in the biological depolymerization and upcycling of polyethylene terephthalate	20
Technologies for the discovery of G protein–coupled receptor–targeting biologics	20
Antimicrobial polymeric biomaterials based on synthetic, nanotechnology, and biotechnological approaches	20
The double life of CRISPR–Cas13	20
Molecular biotechnology for the biodegradation of organofluorine compounds	20
Genetically pliable green algae for bioproduction of modified fatty acids, nutritional therapeutic oils, and biopharmaceuticals	20
Resolving misconceptions and constraints in growth-coupled bioproduction	20
Optogenetics in medicine: innovations and therapeutic applications	19
Systems approaches for optimizing CO2 fixation pathways	19
Sharp solutions to cleave plant fibers	19
Window of sustainable bioprocess operation: towards merging environmental sustainability assessment and process operation at early-stage bioprocess development	19
Carbon-efficient waste upcycling: combining syngas fermentation and chain elongation with synthetic consortia	19
Unlocking therapeutic impacts of the gut microbiota with computational tools	19
Engineering metalloproteinase inhibitors: tissue inhibitors of metalloproteinases or antibodies, that is the question	18
Rational design of peptide-based implants for corneal bioengineering	18
Cell-free chemoenzymatic cascades with bio-based molecules	18
Food industry side streams: an unexploited source for biotechnological phosphorus upcycling	18
Advancing fungal biodegradation of nonsteroidal anti-inflammatory drugs — challenges and future perspectives	18
Controlled protein synthesis and spatial organisation in microfluidic environments	18
Precise control methods of the physicochemical properties of nanoparticles for personalized medicine	18
Engineered microbial living matter for diagnostics, prevention, and therapy	18
Engineering ribosomally synthesized and posttranslationally modified peptides as new antibiotics	18
Cutting-edge plant natural product pathway elucidation	18
Minimal enzyme cascades for the aromatic-to-aromatic upgrading of lignin monomers	17
Phage biocontrol for reducing bacterial foodborne pathogens in produce and other foods	17
Technoeconomic analysis for near-term scale-up of bioprocesses	17
Editorial overview: 21st Century solutions for crop improvement	17
Valorization of waste biomass in the circular bioeconomy: advances in pretreatment and catalytic conversion technologies in modern biorefineries	17
Toward manipulating serotonin signaling via the microbiota–gut–brain axis	17
Cell-free protein synthesis systems for vaccine design and production	17
Spatial omics techniques and data analysis for cancer immunotherapy applications	17
Recent developments of oleaginous yeasts toward sustainable biomanufacturing	17
Editorial overview: Unlocking the secrets of nongenetic plasticity, one cell at a time	16
Dissecting postharvest chilling injury through biotechnology	16
Process intensification in continuous flow biocatalysis by up and downstream processing strategies	16
Cell–nano interactions of polydopamine nanoparticles	16
Tissue-resident macrophages — early passengers or drivers in the tumor niche?	16
Glycosyltransferases in human milk oligosaccharide synthesis: structural mechanisms and rational design	15
The dawn of interoperating spatial models in cell biology	15
Upstream design enabling downstream simplification in biomanufacturing: strategies and perspectives	15
Pseudomonas putida as a synthetic biology chassis and a metabolic engineering platform	15
Editorial Board	15
Biotechnological interventions in reducing losses of tropical fruits and vegetables	15
Biological recognition and cellular trafficking of targeted RNA-lipid nanoparticles	15
From design–build–test–learn cycles to AI-driven digital twins for bioprocess scale-up in the Genesis Mission era	15
Constructing next-generation CRISPR–Cas tools from structural blueprints	15
Coupling and uncoupling growth and product formation for producing chemicals	15
Advancing in vitro gut fermentation: the convergence of gut-on-a-chip and digital twins	15
Harnessing the omics revolution to address the global biodiversity crisis	15
AuxSynBio: synthetic biology tools to understand and engineer auxin	14
The tier system: a host development framework for bioengineering	14
N-acetyl-aspartate metabolism at the interface of cancer, immunity, and neurodegeneration	14
Advances in genome-scale metabolic models of industrially important fungi	14
Disturbance and stability dynamics in microbial communities for environmental biotechnology applications	14
Bacteriophage nanoparticles: an emerging modular delivery platform	14
Advances in designed bionanomolecular assemblies for biotechnological and biomedical applications	14
Emerging strategies to enhance microbial natural product–based drug discovery	14
Mannosylerythritol lipids: production, downstream processing, and potential applications	13
Large language model agents as experimental orchestrators in data-driven enzyme engineering	13
Editorial Board	13
Ionizable lipids empowered with artificial intelligence for mRNA delivery	13
From transport to regulation: systems engineering for high-efficiency dicarboxylic acid biosynthesis	13
Editorial Board	13
Thermodynamic tools for more efficient biotechnological processes: an example in poly-(3-hydroxybutyrate) production from carbon monoxide	13
Transforming stressed plants into healthy foods	13
Advancing plant science through precision 3D bioprinting: new tools for research and biotech applications	13
Beyond co-expression: pathway discovery for plant pharmaceuticals	13
Toward molecular classification architectures for cell-free diagnostics	13
Harnessing Fe(II)/α-ketoglutarate-dependent oxygenases for structural diversification of fungal meroterpenoids	13
Advances in mass spectrometry-enabled multiomics at single-cell resolution	13
Corrigendum to “Advances in engineering substrate scope of Pseudomonas cell factories” [Curr Opin Biotechnol 92 (2025) 103270]	13
Using systems metabolic engineering strategies for high-oil maize breeding	13
Design and fabrication of field-deployable microbial biosensing devices	13
Biotechnological polyphosphate as an opportunity to contribute to the circularization of the phosphate economy	13
Harnessing noncanonical redox cofactors to advance synthetic assimilation of one-carbon feedstocks	12
Detecting antibiotic resistance genes in anthropogenically impacted streams and rivers	12
Corrigendum to: “Advances in consolidated bioprocessing using synthetic cellulosomes” [Curr Opin Biotechnol 2022, 78:102840]	12