Current Opinion in Biotechnology

Article	Citations
Editorial Board	292
Editorial Board	233
Corrigendum to “Methylotrophs: from C1 compounds to food” [Curr Opin Biotechnol 75 (2022) 102685]	180
How much could improving photosynthesis increase crop yields? A call for systems-level perspectives to guide engineering strategies	174
Stem cells characterization: OMICS reinforcing analytics	117
Systems biology-based analysis of cell-free systems	108
Recent progress in the synthesis of advanced biofuel and bioproducts	105
The journey of a lifetime — development of Pfizer’s COVID-19 vaccine	101
Cell-free technologies for biopharmaceutical research and production	99
Synthetic biology advances towards a bio-based society in the era of artificial intelligence	96
The osteo-angiogenic signaling crosstalk for bone regeneration: harmony out of complexity	92
Circadian rhythms and meal timing: impact on energy balance and body weight	91
Applications of artificial intelligence to enzyme and pathway design for metabolic engineering	88
Tumor lipid metabolism: a mechanistic link between diet and cancer progression	87
Bioelectrosynthesis systems	86
Creating value from purple phototrophic bacteria via single-cell protein production	85
Editorial Board	82
Innovations and trends in antibody repertoire analysis	81
Engineering of new-to-nature ribosomally synthesized and post-translationally modified peptide natural products	78
Engineering the plant microbiota in the context of the theory of ecological communities	78
Engineered T cells and macrophages: two arms to seize solid tumors	77
Leveraging rAAV bioprocess understanding and next generation bioanalytics development	76
Whole-plant phenotypic engineering: moving beyond ratios for multi-objective optimization of nutrient use efficiency	74
Bridging the gap — biomimetic design of bioelectronic interfaces	74
Coupling chemistry and biology for the synthesis of advanced bioproducts	72

Emerging technologies for genetic control systems in cellular therapies	70
Engineered living bioassemblies for biomedical and functional material applications	68
A molecular assessment of the practical potential of DNA-based computation	67
Lighting the way: recent developments and applications in molecular optogenetics	63
The plant microbiota: composition, functions, and engineering	63
Recent advances, opportunities and challenges in cybergenetic identification and control of biomolecular networks	61
Single-cell analysis opens a goldmine for plant functional studies	61
Genetically encoded elastin-like polypeptide nanoparticles for drug delivery	59
Fermented food products in the era of globalization: tradition meets biotechnology innovations	59
Deep learning in regulatory genomics: from identification to design	58
2-hydroxyglutarate rides the cancer-immunity cycle	56
Emerging trends in production of plant natural products and new-to-nature biopharmaceuticals in yeast	56
Multifaceted metabolic role of infections in the tumor microenvironment	56
Biocatalytic cascade transformations for the synthesis of C-nucleosides and N-nucleoside analogs	55
Engineering of the genetic code	52
Regulatory roles of microRNAs in insect pests: prospective targets for insect pest control	52
Computational tools and resources for designing new pathways to small molecules	51
Perspectives for self-driving labs in synthetic biology	51
Machine learning to navigate fitness landscapes for protein engineering	51
Dietary microalgae on poultry meat and eggs: explained versus unexplained effects	51
Using genome and transcriptome analysis to elucidate biosynthetic pathways	48
Contemporary aquaculture: implications for human nutrition	47
Mass spectrometry imaging methods for visualizing tumor heterogeneity	47
Editorial overview: Biotechnological insights of marine and terrestrial microbiome	46
Tissue engineering in the agri-food industry: current status, socio-economic overview and regulatory compliance	45
Editorial Board	45
Computational approaches to understanding nutrient metabolism and metabolic disorders	45
Editorial Board	45
Harnessing in vitro platforms for natural product research: in vitro driven rational engineering and mining (iDREAM)	44
Reducing crop losses by gene-editing control of organ developmental physiology	44
Toolboxes for plant systems biology research	44
Analytics of host cell proteins (HCPs): lessons from biopharmaceutical mAb analysis for Gene therapy products	44
Integration of omics data to unravel root microbiome recruitment	43
Plant synthetic biology as a tool to help eliminate hidden hunger	42
Anaerobic fluorescent reporters for cell identification, microbial cell biology and high-throughput screening of microbiota and genomic libraries	42
Matching diverse feedstocks to conversion processes for the future bioeconomy	41
Advancing in vivo reprogramming with synthetic biology	41
Corrigendum to: “Design, construction, and application of noncanonical redox cofactor infrastructures” [Curr Opin Biotechnol 84 (2023) 103019]	40
Sourcing DNA parts for synthetic biology applications in plants	40
Editorial overview: Emerging routes to sustainable energy capture and conversion into value-added products	40
The potential of amoeba-based processes for natural product syntheses	40
Synthetic biology approaches for dynamic CHO cell engineering	39
Single-cell omics analysis with genome-scale metabolic modeling	39
Biochemical production with microbial bioelectrochemical systems	39
Editorial overview: Pioneering innovations in food biotechnology	39
Low-cost, point-of-care biomarker quantification	38
Metabolic and cellular engineering for the production of natural products	38
Understanding atmospheric intercontinental dispersal of harmful microorganisms	38
Synthetic apomixis: the beginning of a new era	38
Recent advances in biotechnology for marine enzymes and molecules	38

Redox as a bioprocess parameter: analytical redox quantification in biological therapeutic production	38
Impact of anthropogenic contamination on glacier surface biota	37
Overview of dietary assessment methods for measuring intakes of foods, beverages, and dietary supplements in research studies	37
Matchmaking at the cell surface using bispecifics to put cells on their best behavior	37
Editorial overview: Nanobiotechnology for immunoengineering	36
Editorial Board	36
The aging tumor metabolic microenvironment	36
Complexity of subcellular metabolism: strategies for compartment-specific profiling	36
Antigen-specific nanomedicines for the treatment of autoimmune disease: target cell types, mechanisms and outcomes	35
Understanding and harnessing the complexity of interspecies interactions in acetogenic mixotrophic co-cultures	35
Strategies and opportunities for engineering antifungal peptides for therapeutic applications	35
Targeting ripening regulators to develop fruit with high quality and extended shelf life	34
Engineering insect resistance using plant specialized metabolites	34
Next-generation biological vector platforms for in vivo delivery of genome editing agents	34
Extracellular vesicles as therapeutics for inflammation and infection	34
What’s new in single-cell proteomics	34
Synthetic fuels: what are they and where do they come from?	34
Engineered methane biocatalysis: strategies to assimilate methane for chemical production	33
Editorial Board	33
Potential use of microbial engineering in single-cell protein production	33
Synthetic mRNA – emerging new class of drug for tissue regeneration	33
Editorial overview: Biotechnology to help understand and harness biotic interactions in plants	33
Programmable synthetic biology tools for developing microbial cell factories	33
Ketogenic regimens for acute neurotraumatic events	33
Strategies for uncovering stable isotope tracing patterns between cell populations	33
Inhalable mRNA vaccines for respiratory diseases: a roadmap	32
Challenges and future perspectives for high-throughput chimeric antigen receptor T cell discovery	32
Biotechnology approaches for natural product discovery, engineering, and production based on Burkholderia bacteria	32
Biohybrid approaches to interface with the nervous system: the best of both worlds	32
Bridging the gender gap in autoimmunity with T-cell–targeted biomaterials	32
Practical monitoring technologies for cells and substrates in biomanufacturing	32
Optimizing the value of bioinks and robotics to advance in vivo bioprinting	32
Avoidance of detrimental defense responses in beneficial plant–microbe interactions	31
Engineering bacterial warriors: harnessing microbes to modulate animal physiology	31
Can digital twin efforts shape microorganism-based alternative food?	31
Microbiological food safety considerations in shelf-life extension of fresh fruits and vegetables	30
Beyond vancomycin: recent advances in the modification, reengineering, production and discovery of improved glycopeptide antibiotics to tackle multidrug-resistant bacteria	30
Editorial Board	30
Analytical tools for unravelling the metabolism of gas-fermenting Clostridia	30
De novo domestication: retrace the history of agriculture to design future crops	30
Editorial overview: Tissue, cell, and pathway engineering	30
Large-scale production of enzymes for biotechnology uses	30
Machine learning for predicting phenotype from genotype and environment	30
Two in one: the emerging concept of bifunctional antibodies	29
Mycotoxin risks are lower in biotech corn	29
Biologics-based degraders — an expanding toolkit for targeted-protein degradation	29
Life stage-dependent genetic traits as drivers of plant–herbivore interactions	29
Senescent cells in tissue engineering	29
Genetic and process engineering for polyhydroxyalkanoate production from pre- and post-consumer food waste	29
An integrated eco-evolutionary framework to predict population-level responses of climate-sensitive pathogens	29
In situ characterization of the tumor microenvironment	29
Sustainability in food-waste reduction biotechnology: a critical review	29
Engineering plant–microbe communication for plant nutrient use efficiency	28
Clustered regularly interspaced short palindromic repeats tools for plant metabolic engineering: achievements and perspectives	28
Engineering next-generation chimeric antigen receptor-T cells: recent breakthroughs and remaining challenges in design and screening of novel chimeric antigen receptor variants	28
Achieving net zero CO2 emission in the biobased production of reduced platform chemicals using defined co-feeding of methanol	28
Searching for the optimal microbial factory: high-throughput biosensors and analytical techniques for screening small molecules	28
Advances in engineering and optimization of transcription factor-based biosensors for plug-and-play small molecule detection	28
Cell-free synthesis of industrial chemicals and biofuels from carbon feedstocks	28
Corrigendum to “Unraveling the mechanobiology of immune cells” [Curr Opin Biotechnol 66 (2020) 236-245]	28
Quantitative and analytical tools to analyze the spatiotemporal population dynamics of microbial consortia	28
Recent trends in the modification of polyphenolic compounds using hydroxylation and glycosylation	27
General framework for enzyme-photo-coupled catalytic system toward carbon dioxide conversion	27
Advances in engineering substrate scope of Pseudomonas cell factories	27
Progress and challenges in the translation of cancer nanomedicines	27
Division of labor for substrate utilization in natural and synthetic microbial communities	27
Decoding dissolved information: environmental DNA sequencing at global scale to monitor a changing ocean	26
Force in numbers: high-throughput screening approaches to unlock microbial transport	26
Advances in engineering algae for biofuel production	25
Hitting the sweet spot with capillary electrophoresis: advances in N-glycomics and glycoproteomics	25
Engineering bacteria for cancer immunotherapy	25
Drugging the microbiome and bacterial live biotherapeutic consortium production	25
Cellobiose dehydrogenase in biofuel cells	25
Computational models in systems biology: standards, dissemination, and best practices	25
Proteomics insights into the fungal-mediated bioremediation of environmental contaminants	25
Combining enzyme and metabolic engineering for microbial supply of therapeutic phytochemicals	25
Engineering microbial consortia with rationally designed cellular interactions	25
Chemical speciation of trace metals in mammalian cell culture media: looking under the hood to boost cellular performance and product quality	25
Recent advances in microbial synthesis of polyphenols	25
Integrating bioprinting and optogenetic technologies for precision plant tissue engineering	25

Path to zero emission of nitrous oxide in sewage treatment: is nitrification controllable or avoidable?	25
Carbon recycling with synthetic CO2 fixation pathways	25
Biomolecular feedback controllers: from theory to applications	25
Cytochromes P450 in the biocatalytic valorization of lignin	24
Enzymes in biotechnology: Critical platform technologies for bioprocess development	24
Cell-free enzyme cascades — application and transition from development to industrial implementation	24
Quantifying nanoparticle delivery: challenges, tools, and advances	24
Machine learning applications to improve flavor and nutritional content of horticultural crops through breeding and genetics	24
Approaches for a more microbiologically and chemically safe dried fruit supply chain	24
Continuous biomanufacturing with microbes — upstream progresses and challenges	24
In vivo 2H/13C flux analysis in metabolism research	23
Building mutational bridges between carbohydrate-active enzymes	23
Transcription factor-based biosensors: a molecular-guided approach for natural product engineering	23
Getting better all the time — recent progress in the development of CRISPR/Cas-based tools for plant genome engineering	23
RNAs — a new frontier in crop protection	23
Editorial Board	22
Pleiotropy, a feature or a bug? Toward co-ordinating plant growth, development, and environmental responses through engineering plant hormone signaling	22
Machine learning in wastewater: opportunities and challenges — “not everything is a nail!”	22
Editorial overview: Tissue, cell and pathway engineering: bioinspired, biomimetic and hybrid neural interfaces	22
Technologies for the discovery of G protein–coupled receptor–targeting biologics	22
Optogenetics in medicine: innovations and therapeutic applications	22
Antimicrobial polymeric biomaterials based on synthetic, nanotechnology, and biotechnological approaches	22
Engineering organoids as cerebral disease models	22
Filamentous fungi for future functional food and feed	21
Utilizing cross-species co-cultures for discovery of novel natural products	21
Coordinating precursor supply for pharmaceutical polyketide production in Streptomyces	21
Window of sustainable bioprocess operation: towards merging environmental sustainability assessment and process operation at early-stage bioprocess development	21
SgRNA engineering for improved genome editing and expanded functional assays	21
Editorial overview: Phosphate Special Issue	21
Recent advances in the biological depolymerization and upcycling of polyethylene terephthalate	21
Microbial production of chemicals driven by CRISPR-Cas systems	21
Technoeconomic analysis for near-term scale-up of bioprocesses	20
Time to integrate biotechnological approaches into fish gut microbiome research	20
Editorial overview: 21st Century solutions for crop improvement	20
Identification of novel membrane proteins for improved lignocellulose conversion	20
Microalgal and bacterial auxin biosynthesis: implications for algal biotechnology	20
Engineered microbial living matter for diagnostics, prevention, and therapy	20
Cutting-edge plant natural product pathway elucidation	20
Food industry side streams: an unexploited source for biotechnological phosphorus upcycling	20
Industrial potential of the enzymatic synthesis of nucleoside analogs: existing challenges and perspectives	20
The double life of CRISPR–Cas13	20
Ultrasonographic features of ovarian morphology capture nutritional and metabolic influences on the reproductive axis: implications for biomarker development in ovulatory disorders	20
Petrosia ficiformis (Poiret, 1789): an excellent model for holobiont and biotechnological studies	19
Spatial omics techniques and data analysis for cancer immunotherapy applications	19
Surveilling cellular vital signs: toward label-free biosensors and real-time viability assays for bioprocessing	19
Cell–nano interactions of polydopamine nanoparticles	19
Toward manipulating serotonin signaling via the microbiota–gut–brain axis	19
Coupling electrochemical CO2 reduction to microbial product generation – identification of the gaps and opportunities	19
Recent developments of oleaginous yeasts toward sustainable biomanufacturing	19
Rational design of peptide-based implants for corneal bioengineering	19
Cell-free protein synthesis systems for vaccine design and production	19
Advancing fungal biodegradation of nonsteroidal anti-inflammatory drugs — challenges and future perspectives	19
Phage biocontrol for reducing bacterial foodborne pathogens in produce and other foods	18
Tissue-resident macrophages — early passengers or drivers in the tumor niche?	18
Cell-free chemoenzymatic cascades with bio-based molecules	18
Editorial Board	18
Transdermal sensing: in-situ non-invasive techniques for monitoring of human biochemical status	18
Precise control methods of the physicochemical properties of nanoparticles for personalized medicine	18
Process intensification in continuous flow biocatalysis by up and downstream processing strategies	18
Bio-integrative design of the neural tissue-device interface	18
Dissecting postharvest chilling injury through biotechnology	18
Editorial overview: Unlocking the secrets of nongenetic plasticity, one cell at a time	18
Engineering ribosomally synthesized and posttranslationally modified peptides as new antibiotics	18
Engineering metalloproteinase inhibitors: tissue inhibitors of metalloproteinases or antibodies, that is the question	18
Sharp solutions to cleave plant fibers	18
Artificial intelligence: a solution to involution of design–build–test–learn cycle	18
The dawn of interoperating spatial models in cell biology	18
Adaptive biomimicry: design of neural interfaces with enhanced biointegration	17
Pseudomonas putida as a synthetic biology chassis and a metabolic engineering platform	17
Glycosyltransferases in human milk oligosaccharide synthesis: structural mechanisms and rational design	17
Coupling and uncoupling growth and product formation for producing chemicals	17
Microenvironmental effects can masquerade as substrate channelling in cascade biocatalysis	17
Biological recognition and cellular trafficking of targeted RNA-lipid nanoparticles	17
Constructing next-generation CRISPR–Cas tools from structural blueprints	17
Harnessing the omics revolution to address the global biodiversity crisis	17
Recent advances in synthetic glycoengineering for biological applications	17
Coral holobionts and biotechnology: from Blue Economy to coral reef conservation	17
Editorial overview: Pharmaceutical biotechnology	17
Biotechnological interventions in reducing losses of tropical fruits and vegetables	17
Integrative metabolic flux analysis reveals an indispensable dimension of phenotypes	17
Advances in designed bionanomolecular assemblies for biotechnological and biomedical applications	16
Different avenues for progress apply to drought tolerance, water use efficiency and yield in dry areas	16
Disturbance and stability dynamics in microbial communities for environmental biotechnology applications	16
Engineering of citrus to obtain huanglongbing resistance	16
AuxSynBio: synthetic biology tools to understand and engineer auxin	16
N-acetyl-aspartate metabolism at the interface of cancer, immunity, and neurodegeneration	16
Mannosylerythritol lipids: production, downstream processing, and potential applications	16
Advances in genome-scale metabolic models of industrially important fungi	16
Harnessing Fe(II)/α-ketoglutarate-dependent oxygenases for structural diversification of fungal meroterpenoids	16
The tier system: a host development framework for bioengineering	16
Cyanobacteria as cell factories: the roles of host and pathway engineering and translational research	16
Biotechnological polyphosphate as an opportunity to contribute to the circularization of the phosphate economy	15
Corrigendum to “Advances in engineering substrate scope of Pseudomonas cell factories” [Curr Opin Biotechnol 92 (2025) 103270]	15
Calm and Frenzy: marine obligate hydrocarbonoclastic bacteria sustain ocean wellness	15
Using systems metabolic engineering strategies for high-oil maize breeding	15