Current Opinion in Biotechnology

Article	Citations
Deep learning for optimization of protein expression	271
Recent progress in the synthesis of advanced biofuel and bioproducts	213
Inhalable mRNA vaccines for respiratory diseases: a roadmap	173
Enhancing phage therapy through synthetic biology and genome engineering	151
Beyond vancomycin: recent advances in the modification, reengineering, production and discovery of improved glycopeptide antibiotics to tackle multidrug-resistant bacteria	114
Microenvironmental effects can masquerade as substrate channelling in cascade biocatalysis	112
Optimizing the value of bioinks and robotics to advance in vivo bioprinting	103
Editorial Reflections on BioEnergy: Perspectives from 2024	102
The yin and yang of itaconate metabolism and its impact on the tumor microenvironment	101
Avoidance of detrimental defense responses in beneficial plant–microbe interactions	96
Editorial overview: Biotechnology to help understand and harness biotic interactions in plants	92
Novel transducers for high-channel-count neuroelectronic recording interfaces	92
Bioelectrosynthesis systems	91
Editorial Board	90
Editorial Board	83
Editorial overview: Tissue, cell, and pathway engineering: Recent innovations in biomedical technologies	81
Autophagy as a critical driver of metabolic adaptation, therapeutic resistance, and immune evasion of cancer	80
Tumor lipid metabolism: a mechanistic link between diet and cancer progression	79
Editorial Board	75
Systems biology-based analysis of cell-free systems	75
Recent advances of machine learning applications in human gut microbiota study: from observational analysis toward causal inference and clinical intervention	75
Editorial overview: Analytical biotechnology for healthcare, strain engineering, biosensing and synthetic biology	75
Recent advances in microbial community analysis from machine learning of multiparametric flow cytometry data	74
Editorial Board	73
Biotechnological interventions in reducing losses of tropical fruits and vegetables	72

Integrative metabolic flux analysis reveals an indispensable dimension of phenotypes	72
Isotope tracing in health and disease	71
Adaptive biomimicry: design of neural interfaces with enhanced biointegration	71
Recent advances in bivalve-microbiota interactions for disease prevention in aquaculture	69
Editorial overview: Unlocking the secrets of nongenetic plasticity, one cell at a time	67
Stem cell engineering approaches for investigating glial cues in central nervous system disorders	67
Biological recognition and cellular trafficking of targeted RNA-lipid nanoparticles	64
The osteo-angiogenic signaling crosstalk for bone regeneration: harmony out of complexity	61
Coral holobionts and biotechnology: from Blue Economy to coral reef conservation	60
Application of bacteriophage-derived endolysins to combat streptococcal disease: current state and perspectives	59
Biotechnology approaches for natural product discovery, engineering, and production based on Burkholderia bacteria	58
Constructing next-generation CRISPR–Cas tools from structural blueprints	58
The dawn of interoperating spatial models in cell biology	58
Progress toward rapid, at-line N-glycosylation detection and control for recombinant protein expression	57
Toward optimal use of biomass as carbon source for chemical bioproduction	57
Harnessing the omics revolution to address the global biodiversity crisis	57
Bio-based antimicrobial compositions and sensing technologies to improve food safety	57
Microbial single-cell mass spectrometry: status, challenges, and prospects	55
Engineering protein nanoparticles for drug delivery	55
Bridging the gender gap in autoimmunity with T-cell–targeted biomaterials	53
Mineralized collagen scaffolds for regenerative engineering applications	52
Coupling and uncoupling growth and product formation for producing chemicals	51
The prospect of superior yeast for winemaking: recent successes through bioprospecting	50
Jump-starting chimeric antigen receptor-T cells to go the extra mile with nanotechnology	50
Methane to bioproducts: unraveling the potential of methanotrophs for biomanufacturing	50
Challenges and future perspectives for high-throughput chimeric antigen receptor T cell discovery	49
Biofabrication strategies for cardiac tissue engineering	48
Bioengineering applications for hearing restoration: emerging biologically inspired and biointegrated designs	48
Biotechnology for secure biocontainment designs in an emerging bioeconomy	46
Circadian rhythms and meal timing: impact on energy balance and body weight	45
Illuminating the cross-talk between tumor metabolism and immunity in IDH-mutated cancers	45
Assessing the potential to harness the microbiome through plant genetics	44
Continuous Plankton Recorder in the omics era: from marine microbiome to global ocean observations	44
Pathway elucidation and engineering of plant-derived diterpenoids	44
An ossifying landscape: materials and growth factor strategies for osteogenic signalling and bone regeneration	43
Engineering healthy crops: molecular strategies for enhancing the plant immune system	43
Plant virus-interactions: unraveling novel defense mechanisms under immune-suppressing pressure	43
NLRP3 as a sensor of metabolism gone awry	43
Stem cells characterization: OMICS reinforcing analytics	43
Native LC–MS for capturing quality attributes of biopharmaceuticals on the intact protein level	42
Aspergillus terreus as an industrial filamentous fungus for pharmaceutical biotechnology	42
Biohybrid approaches to interface with the nervous system: the best of both worlds	41
Transdermal sensing: in-situ non-invasive techniques for monitoring of human biochemical status	41
Engineering bacterial warriors: harnessing microbes to modulate animal physiology	40
Using plant chemistry to improve interactions between plants, herbivores and their natural enemies: challenges and opportunities	40
Recent progress and advanced technology in carbohydrate-based drug development	39
Phage-based target discovery and its exploitation towards novel antibacterial molecules	39
Engineering highly productive cyanobacteria towards carbon negative emissions technologies	39
Roots of synthetic ecology: microbes that foster plant resilience in the changing climate	38
Recent advances in synthetic glycoengineering for biological applications	38

Advances in quantifying circulatory microRNA for early disease detection	37
Editorial overview: The future of food biotechnology is microbial	37
Industrializing methanotrophs and other methylotrophic bacteria: from bioengineering to product recovery	36
Biotic–abiotic hybrids for bioanalytics and biocatalysis	36
Corrigendum to “Methylotrophs: from C1 compounds to food” [Curr Opin Biotechnol 75 (2022) 102685]	36
Strategies for engineering improved nitrogen use efficiency in crop plants via redistribution and recycling of organic nitrogen	36
Analytical tools for unravelling the metabolism of gas-fermenting Clostridia	35
Tumor interstitial fluid analysis enables the study of microenvironment–cell interactions in cancers	35
Synthetic or natural? Metabolic engineering for assimilation and valorization of methanol	35
Cell-free technologies for biopharmaceutical research and production	35
Advances in consolidated bioprocessing using synthetic cellulosomes	35
Strategies for uncovering stable isotope tracing patterns between cell populations	35
The phosphorus challenge: biotechnology approaches for a sustainable phosphorus system	35
Synthetic molecular evolution of antimicrobial peptides	34
De novo domestication: retrace the history of agriculture to design future crops	34
The limitless applications of single-cell metabolomics	34
Chitosan and other edible coatings to extend shelf life, manage postharvest decay, and reduce loss and waste of fresh fruits and vegetables	33
Creating an atlas to visualize the biodiversity of the mammalian gut microbiota	33
Synthetic biology advances towards a bio-based society in the era of artificial intelligence	33
Role of biotechnology in the advancement of biodegradable polymers and functionalized additives for food packaging systems	32
Large-scale production of enzymes for biotechnology uses	32
Toward sustainable, cell-free biomanufacturing	32
Practical monitoring technologies for cells and substrates in biomanufacturing	32
Stop helping pathogens: engineering plant susceptibility genes for durable resistance	32
How much could improving photosynthesis increase crop yields? A call for systems-level perspectives to guide engineering strategies	32
Mining natural products for advanced biofuels and sustainable bioproducts	32
Virome studies of food production systems: time for ‘farm to fork’ analyses	32
Pseudomonas putida as a synthetic biology chassis and a metabolic engineering platform	32
Can digital twin efforts shape microorganism-based alternative food?	31
Microbiological food safety considerations in shelf-life extension of fresh fruits and vegetables	31
Advances in automated and reactive flow cytometry for synthetic biotechnology	31
Machine learning for predicting phenotype from genotype and environment	31
Recent advances of surface acoustic wave-based sensors for noninvasive cell analysis	31
Enhancing phytoremediation of soils polluted with heavy metals	30
Mediated electrochemistry for redox-based biological targeting: entangling sensing and actuation for maximizing information transfer	30
Creating value from purple phototrophic bacteria via single-cell protein production	30
Recent advances in heterologous expression of natural product biosynthetic gene clusters in Streptomyces hosts	30
Barriers to microalgal mass cultivation	30
The journey of a lifetime — development of Pfizer’s COVID-19 vaccine	30
Biosynthesis of human milk oligosaccharides via metabolic engineering approaches: current advances and challenges	30
Metabolic engineering and fermentation of microorganisms for carotenoids production	30
Editorial Board	29
Enzymatic and non-enzymatic functional attributes of plant microbiome	29
Applications of artificial intelligence to enzyme and pathway design for metabolic engineering	29
Food value chain interventions and nutritional outcomes: a review of evidence and recommendations for future assessments	29
Production of therapeutic glycoproteins in glycoengineered plant: old farm for new crops	28
A molecular assessment of the practical potential of DNA-based computation	28
Editorial overview: Tumor–stroma crosstalk: Shaping and characterizing the metabolic microenvironment of tumors	28
Progress and challenges in the translation of cancer nanomedicines	28
Challenges and opportunities in biological funneling of heterogeneous and toxic substrates beyond lignin	28
Bacterial microcompartment architectures as biomaterials for conversion of gaseous substrates	28
Advances in engineering and optimization of transcription factor-based biosensors for plug-and-play small molecule detection	28
Editorial Board	28
Resolving cellular dynamics using single-cell temporal transcriptomics	28
MS-based technologies for untargeted single-cell proteomics	28
Applications of targeted proteomics in metabolic engineering: advances and opportunities	27
Engineering of citrus to obtain huanglongbing resistance	27
Editorial Board	27
Pulses-derived proteins for the plant-based market: opportunities to reduce postharvest loss and waste	27
Current research approaches in downstream processing of pharmaceutically relevant proteins	27
Editorial Board	27
Putting the pieces together: mapping the O-glycoproteome	27
Editorial overview: Tissue, cell, and pathway engineering	27
Editorial Board	27
Editorial overview: Pharmaceutical biotechnology	27
Editorial Board	26
Editorial Board	26
Biotechnological approaches for predicting and controlling apple storage disorders	26
Editorial overview: Pharmaceutical biotechnology: the pandemic years — new modality development and biomanufacturing innovations in a maturing field	26
Editorial overview: Chemical Biotechnology	26
Biomaterial approaches for engineering and analyzing structure and metabolic states of microbial consortia within biofilms	25
Recent advances, opportunities and challenges in cybergenetic identification and control of biomolecular networks	25
Upstream continuous processing: recent advances in production of biopharmaceuticals and challenges in manufacturing	25
Whole-plant phenotypic engineering: moving beyond ratios for multi-objective optimization of nutrient use efficiency	25
Nanoencapsulation application to prolong postharvest shelf life	24
Leveraging rAAV bioprocess understanding and next generation bioanalytics development	24
Single-cell analysis opens a goldmine for plant functional studies	24
Balancing safety and efficacy: tuning the biodistribution and pharmacokinetics of cytokine immunotherapies	24
Deep learning in regulatory genomics: from identification to design	24
Advances in mass spectrometry-enabled multiomics at single-cell resolution	23

Engineering plant–microbe communication for plant nutrient use efficiency	23
Innovations and trends in antibody repertoire analysis	23
Building Synthetic Yeast Factories to Produce Fat-soluble Antioxidants	23
Boundary flux analysis: an emerging strategy for investigating metabolic pathway activity in large cohorts	23
Recent advances in prime editing technologies and their promises for therapeutic applications	23
Two in one: the emerging concept of bifunctional antibodies	23
Biomanufacturing of value-added chemicals from lignin	23
Designer protein compartments for microbial metabolic engineering	23
In situ characterization of the tumor microenvironment	23
Future directions in microbial nitrogen cycling in wastewater treatment	23
Editorial overview: Nanobiotechnology: A focus on the fundamentals	23
Editorial	22
Manufactured extracellular vesicles as human therapeutics: challenges, advances, and opportunities	22
Emerging concepts in the semisynthetic and mutasynthetic production of natural products	22
Antifungal natural products	22
Lighting the way: recent developments and applications in molecular optogenetics	22
Resource recovery from food-processing wastewaters in a circular economy: a methodology for the future	22
Biologics-based degraders — an expanding toolkit for targeted-protein degradation	22
Senescent cells in tissue engineering	22
Engineered living bioassemblies for biomedical and functional material applications	22
Harnessing Fe(II)/α-ketoglutarate-dependent oxygenases for structural diversification of fungal meroterpenoids	22
Life stage-dependent genetic traits as drivers of plant–herbivore interactions	22
The role of inositol in the environmental organic phosphate cycle	22
Engineering the plant microbiota in the context of the theory of ecological communities	21
Cell-free synthesis of industrial chemicals and biofuels from carbon feedstocks	21
Discovery of new enzymatic functions and metabolic pathways using genomic enzymology web tools	21
General framework for enzyme-photo-coupled catalytic system toward carbon dioxide conversion	21
Engineering of new-to-nature ribosomally synthesized and post-translationally modified peptide natural products	21
Bridging the gap — biomimetic design of bioelectronic interfaces	21
Transient electronics: new opportunities for implantable neurotechnology	21
Solar salterns as model systems to study the units of bacterial diversity that matter for ecosystem functioning	21
Genetic manipulation of phages for therapy using BRED	21
Beyond calorie restriction: aging as a biological target for nutrient therapies	20
Current advances in engineering cyanobacteria and their applications for photosynthetic butanol production	20
Engineered diets to improve cancer outcomes	20
Discovery and application of dietary compounds to optimize human health, a focus on skeletal muscle regeneration	20
Editorial overview: Energy biotechnology as an integral solution to global challenges	20
TCA cycle signalling and the evolution of eukaryotes	20
An integrated eco-evolutionary framework to predict population-level responses of climate-sensitive pathogens	20
Corrigendum to “Unraveling the mechanobiology of immune cells” [Curr Opin Biotechnol 66 (2020) 236-245]	19
New strategies to advance plant transformation	19
Engineering next-generation chimeric antigen receptor-T cells: recent breakthroughs and remaining challenges in design and screening of novel chimeric antigen receptor variants	19
Engineering the supernatural: monoclonal antibodies for challenging infectious diseases	19
Genetically encoded elastin-like polypeptide nanoparticles for drug delivery	19
Thermodynamic tools for more efficient biotechnological processes: an example in poly-(3-hydroxybutyrate) production from carbon monoxide	19
Three dimensional bioelectronic interfaces to small-scale biological systems	19
New opportunities for biologically and chemically mediated adsorption and precipitation of phosphorus from wastewater	19
Enhancing enzymatic performance with nanoparticle immobilization: improved analytical and control capability for synthetic biochemistry	19
Glutathione and its precursors in cancer	19
AuxSynBio: synthetic biology tools to understand and engineer auxin	19
Metabolic engineering of microorganisms for carbon dioxide utilization	19
The tier system: a host development framework for bioengineering	19
Micro-scaffolds as synthetic cell niches: recent advances and challenges	19
Phosphorus fertilizer: from commodity to speciality — from fertilizing the field to fertilizing the plant	19
Advances in designed bionanomolecular assemblies for biotechnological and biomedical applications	18
Plant synthetic biology: from inspiration to augmentation	18
Protein engineering for electrochemical biosensors	18
Next-generation metabolic models informed by biomolecular simulations	18
Learning from mistakes: challenges in finding holobiont factors from environmental samples and the importance of methodological consistency	18
Decoding dissolved information: environmental DNA sequencing at global scale to monitor a changing ocean	18
Wastewater biorefineries: exploring biological phosphorus removal and integrated recovery solutions	18
Advances in generative modeling methods and datasets to design novel enzymes for renewable chemicals and fuels	18
A biotechnological perspective on sand filtration for drinking water production	18
Clustered regularly interspaced short palindromic repeats tools for plant metabolic engineering: achievements and perspectives	18
N-acetyl-aspartate metabolism at the interface of cancer, immunity, and neurodegeneration	17
Status and challenges of electrical stimulation use in chronic wound healing	17
Coupling chemistry and biology for the synthesis of advanced bioproducts	17
Sustainability in food-waste reduction biotechnology: a critical review	17
Searching for the optimal microbial factory: high-throughput biosensors and analytical techniques for screening small molecules	17
Biosensor-enabled pathway optimization in metabolic engineering	17
Different avenues for progress apply to drought tolerance, water use efficiency and yield in dry areas	17
Division of labor for substrate utilization in natural and synthetic microbial communities	17
Quantitative and analytical tools to analyze the spatiotemporal population dynamics of microbial consortia	17
Innovative nonviral gene delivery strategies for engineering human mesenchymal stem cell phenotypes toward clinical applications	17
Engineering T-cell receptor–like antibodies for biologics and cell therapy	17
Emerging technologies for genetic control systems in cellular therapies	17
Mannosylerythritol lipids: production, downstream processing, and potential applications	17
Advances in engineering substrate scope of Pseudomonas cell factories	16
Advances in algal lipid metabolism and their use to improve oil content	16
Design and engineering of artificial microbial consortia for biohydrogen production	16
Genetic and process engineering for polyhydroxyalkanoate production from pre- and post-consumer food waste	16
Recent trends in the modification of polyphenolic compounds using hydroxylation and glycosylation	16
Bacterial degradation of perfluoroalkyl acids	16
Evaluating functionalities of food components by a model simulating human intestinal microbiota constructed at Kobe University	16
Cyanobacteria as cell factories: the roles of host and pathway engineering and translational research	16
Biotechnology approaches to reduce antimicrobial resistant postharvest pathogens, mycotoxin contamination, and resulting product losses	16
Achieving net zero CO2 emission in the biobased production of reduced platform chemicals using defined co-feeding of methanol	16
‘From metabolism to metabolism’: holistic considerations on B-vitamin interactions, biofortification, and deficiencies	16
Interactions between nanoparticle corona proteins and the immune system	16
Combining biotechnology and evolution for understanding the mechanisms of pollinator attraction	15
Production of recombinant and therapeutic proteins in microalgae	15
Biocatalytic cascade transformations for the synthesis of C-nucleosides and N-nucleoside analogs	15
The resistome of the bovine gastrointestinal tract	15
Biomanufacturing design: reducing the environmental burden	15
Advances in enzymatic and organismal technologies for the recycling and upcycling of petroleum-derived plastic waste	15