Current Opinion in Biotechnology

Article	Citations
The potential of remote sensing and artificial intelligence as tools to improve the resilience of agriculture production systems	194
Enhancing phytoremediation of soils polluted with heavy metals	131
Pollution to products: recycling of ‘above ground’ carbon by gas fermentation	121
Machine learning applications in systems metabolic engineering	118
Principles for designing an optimal mRNA lipid nanoparticle vaccine	110
Design and application of oncolytic viruses for cancer immunotherapy	86
T-cell repertoire analysis and metrics of diversity and clonality	83
Metagenomic tools in microbial ecology research	82
The future of phage biocontrol in integrated plant protection for sustainable crop production	82
Unlocking the next generation of phage therapy: the key is in the receptors	82
Engineering probiotics for therapeutic applications: recent examples and translational outlook	78
Large-scale production of enzymes for biotechnology uses	76
Enhancing phage therapy through synthetic biology and genome engineering	76
Microfluidic systems for cancer diagnostics	73
Marine collagen scaffolds in tissue engineering	70
Microbial production of polyunsaturated fatty acids — high-value ingredients for aquafeed, superfoods, and pharmaceuticals	66
Cross-linking/mass spectrometry to get a closer view on protein interaction networks	65
Advances in microphysiological blood-brain barrier (BBB) models towards drug delivery	64
Caenorhabditis elegans as a model system for human diseases	64
Sensing the unreachable: challenges and opportunities in biofilm detection	64
Microbial production of extremolytes — high-value active ingredients for nutrition, health care, and well-being	63
Network inference in systems biology: recent developments, challenges, and applications	63
Formate dehydrogenases for CO2 utilization	62
Overview of dietary assessment methods for measuring intakes of foods, beverages, and dietary supplements in research studies	61
Implantable brain machine interfaces: first-in-human studies, technology challenges and trends	60

Glutathione and its precursors in cancer	60
Technoeconomic analysis for biofuels and bioproducts	59
Advances in single cell Raman spectroscopy technologies for biological and environmental applications	59
Unraveling the mechanobiology of immune cells	58
Transcription factor-based biosensors: a molecular-guided approach for natural product engineering	58
New-to-nature chemistry from old protein machinery: carbene and nitrene transferases	58
Synthetic co-cultures: novel avenues for bio-based processes	57
Single-cell metabolomics: where are we and where are we going?	57
Microalgae based production of single-cell protein	57
Chain elongators, friends, and foes	56
Recent advances in understanding the ecophysiology of enhanced biological phosphorus removal	56
Bispecific antibodies in cancer immunotherapy	55
RNA-based fluorescent biosensors for live cell imaging of small molecules and RNAs	55
The role of single cell protein in cellular agriculture	54
The plant microbiota: composition, functions, and engineering	53
Current status of pan-genome analysis for pathogenic bacteria	52
Fermented food products in the era of globalization: tradition meets biotechnology innovations	51
Applications of artificial intelligence to enzyme and pathway design for metabolic engineering	51
Circadian rhythms and meal timing: impact on energy balance and body weight	50
Vascularization in skin wound healing: where do we stand and where do we go?	49
Tracing metabolic flux through time and space with isotope labeling experiments	48
New frontiers: harnessing pivotal advances in microbial engineering for the biosynthesis of plant-derived terpenoids	48
Biobanks for life sciences and personalized medicine: importance of standardization, biosafety, biosecurity, and data management	48
Recent advances on constraint-based models by integrating machine learning	48
Toward sustainable, cell-free biomanufacturing	48
Conquering CRISPR: how phages overcome bacterial adaptive immunity	47
Bacteriophage endolysins — extending their application to tissues and the bloodstream	46
Optogenetics and biosensors set the stage for metabolic cybergenetics	46
Microfluidic single-cell analysis in biotechnology: from monitoring towards understanding	46
Enzyme evolution for industrial biocatalytic cascades	46
A guide to deciphering microbial interactions and metabolic fluxes in microbiome communities	45
Contemporary aquaculture: implications for human nutrition	45
RNAs — a new frontier in crop protection	45
Recent advances in heterologous expression of natural product biosynthetic gene clusters in Streptomyces hosts	45
Reprogramming bacteriophage host range: design principles and strategies for engineering receptor binding proteins	45
Innate immune responses triggered by nucleic acids inspire the design of immunomodulatory nucleic acid nanoparticles (NANPs)	45
Carbonic anhydrase for CO2 capture, conversion and utilization	44
Machine learning to navigate fitness landscapes for protein engineering	44
Engineering nanoparticulate vaccines for enhancing antigen cross-presentation	44
High-throughput screening for efficient microbial biotechnology	43
Lysins breaking down the walls of Gram-negative bacteria, no longer a no-go	43
Beyond the semi-synthetic artemisinin: metabolic engineering of plant-derived anti-cancer drugs	43
Transporter engineering in microbial cell factories: the ins, the outs, and the in-betweens	43
The limitless applications of single-cell metabolomics	42
Patient-derived xenografts (PDXs) as model systems for human cancer	41
Requirements for fungal uptake of dsRNA and gene silencing in RNAi-based crop protection strategies	40
Modular Analysis and Design of Biological Circuits	40
Engineering of new-to-nature ribosomally synthesized and post-translationally modified peptide natural products	40
Challenges and opportunities in biological funneling of heterogeneous and toxic substrates beyond lignin	39
Targeting biofilms using phages and their enzymes	39

Systems metabolomics: from metabolomic snapshots to design principles	39
Systems and synthetic biology tools for advanced bioproduction hosts	39
Stop helping pathogens: engineering plant susceptibility genes for durable resistance	38
Accelerating strain engineering in biofuel research via build and test automation of synthetic biology	38
Coordinating precursor supply for pharmaceutical polyketide production in Streptomyces	38
Transcription factor-based biosensors and inducible systems in non-model bacteria: current progress and future directions	37
Microbial production of fatty acids and derivative chemicals	37
Microbes trading electricity in consortia of environmental and biotechnological significance	37
Complexity of macrophage metabolism in infection	37
Discovery of new enzymatic functions and metabolic pathways using genomic enzymology web tools	37
Synthetic biology for protein-based materials	36
Current state of aromatics production using yeast: achievements and challenges	36
Preclinical data and safety assessment of phage therapy in humans	36
Engineering xylose metabolism in yeasts to produce biofuels and chemicals	36
Cryoablation and immunotherapy of cancer	36
Application of nanotechnology in medical diagnosis and imaging	35
Regulatory roles of microRNAs in insect pests: prospective targets for insect pest control	35
Phages to shape the gut microbiota?	35
Current advances in engineering cyanobacteria and their applications for photosynthetic butanol production	35
Towards superior plant-based foods using metabolomics	35
Recent progress and advanced technology in carbohydrate-based drug development	35
Antifungal natural products	34
Enzymes in biotechnology: Critical platform technologies for bioprocess development	34
All systems go: converging synthetic biology and combinatorial treatment for CAR-T cell therapy	34
Metabolic model guided strain design of cyanobacteria	34
The emergence and promise of single-cell temporal-omics approaches	34
Building kinetic models for metabolic engineering	33
Advances in electrochemical cofactor regeneration: enzymatic and non-enzymatic approaches	33
Insect sex pheromone production in yeasts and plants	32
Biosynthesis of terpene compounds using the non-model yeast Yarrowia lipolytica: grand challenges and a few perspectives	32
TCA cycle signalling and the evolution of eukaryotes	32
Industrial production of microbial protein products	32
Time to integrate biotechnological approaches into fish gut microbiome research	32
Organs-on-a-chip as model systems for multifactorial musculoskeletal diseases	32
Advances in G protein-coupled receptor high-throughput screening	32
Status and challenges of electrical stimulation use in chronic wound healing	32
Recent trends in nanopore polymer functionalization	31
Omics-based strategies to discover novel classes of RiPP natural products	31
Precision medicine — networks to the rescue	30
Chitosan and other edible coatings to extend shelf life, manage postharvest decay, and reduce loss and waste of fresh fruits and vegetables	30
Phage engineering and the evolutionary arms race	30
Bioconversion of renewable feedstocks by Rhodococcus opacus	30
Filamentous fungi for future functional food and feed	30
The human gut microbiota during the initial stages of life: insights from bifidobacteria	30
Challenges and advantages of cell therapy manufacturing under Good Manufacturing Practices within the hospital setting	30
New chemistries and enzymes for synthetic genetics	29
Metabolic engineering for production of functional polysaccharides	29
Coupling electrochemical CO2 reduction to microbial product generation – identification of the gaps and opportunities	28
Enzymatic control of product distribution in terpene synthases: insights from multiscale simulations	28
Cascaded valorization of seaweed using microbial cell factories	28
Recent advances in improving metabolic robustness of microbial cell factories	28
Refactoring biosynthetic gene clusters for heterologous production of microbial natural products	28
Diversity: current and prospective secondary metabolites for nutrition and medicine	28
Challenges and opportunities with CRISPR activation in bacteria for data-driven metabolic engineering	28
The sum is greater than the parts: exploiting microbial communities to achieve complex functions	28
RNA nanotechnology in synthetic biology	28
Microalgal and bacterial auxin biosynthesis: implications for algal biotechnology	27
Incorporating biological structure into machine learning models in biomedicine	27
Phage-specific antibodies	27
Beyond antibacterials – exploring bacteriophages as antivirulence agents	27
Resolving complex phenotypes with Raman spectroscopy and chemometrics	27
Bacillus spore awakening: recent discoveries and technological developments	26
Synthetic metabolism for biohalogenation	26
Calm and Frenzy: marine obligate hydrocarbonoclastic bacteria sustain ocean wellness	26
Engineering microbial cell morphology and membrane homeostasis toward industrial applications	26
The role of cuticle in fruit shelf-life	26
Engineering of natural product biosynthesis in Pseudomonas putida	26
Cyanobacteria as cell factories: the roles of host and pathway engineering and translational research	26
Metabolic interactions in beneficial microbe recruitment by plants	26
Combining protein and metabolic engineering to construct efficient microbial cell factories	26
Biosensor-enabled pathway optimization in metabolic engineering	26
Recent advances in bivalve-microbiota interactions for disease prevention in aquaculture	25
CO2-converting enzymes for sustainable biotechnology: from mechanisms to application	25
Siderophore natural products as pharmaceutical agents	25
Gene delivery for immunoengineering	25
Native LC–MS for capturing quality attributes of biopharmaceuticals on the intact protein level	25
Aptamers in RNA-based switches of gene expression	25
Receptor kinases in plant responses to herbivory	25
Microenvironmental effects can masquerade as substrate channelling in cascade biocatalysis	25
Assessing the potential to harness the microbiome through plant genetics	25

Biotechnologies to tackle the challenge of neoantigen identification	25
Engineering long-circulating nanomaterial delivery systems	25
Quantitative analysis of glycine related metabolic pathways for one-carbon synthetic biology	25
Virus-induced metabolic reprogramming and innate sensing hereof by the infected host	24
Chassis engineering for microbial production of chemicals: from natural microbes to synthetic organisms	24
Biotechnology for secure biocontainment designs in an emerging bioeconomy	24
Understanding the impact of age-related changes in the gut microbiome on chronic diseases and the prospect of elderly-specific dietary interventions	24
Molecular characterization of HEK293 cells as emerging versatile cell factories	24
The role of systems biology in developing non-model cyanobacteria as hosts for chemical production	24
Delivery of CRISPR-Cas systems using phage-based vectors	24
Unlocking nature’s biosynthetic potential by directed genome evolution	24
A roadmap to engineering antiviral natural products synthesis in microbes	23
Engineering microbial consortia with rationally designed cellular interactions	23
Genetically-encoded biosensors for analyzing and controlling cellular process in yeast	23
Neural interface systems with on-device computing: machine learning and neuromorphic architectures	23
Strategies for genotype-flexible plant transformation	23
Organoid technology and applications in cancer immunotherapy and precision medicine	23
Utilizing cross-species co-cultures for discovery of novel natural products	23
Division of labor for substrate utilization in natural and synthetic microbial communities	23
Aspergillus terreus as an industrial filamentous fungus for pharmaceutical biotechnology	23
Polyketide pesticides from actinomycetes	22
Enhancing enzymatic performance with nanoparticle immobilization: improved analytical and control capability for synthetic biochemistry	22
Probiotics for coral aquaculture: challenges and considerations	22
Calling for a systems approach in microbiome research and innovation	22
Modeling of interspecies electron transfer in anaerobic microbial communities	22
Engineering of citrus to obtain huanglongbing resistance	22
Process intensification in continuous flow biocatalysis by up and downstream processing strategies	22
Engineering natural and noncanonical nicotinamide cofactor-dependent enzymes: design principles and technology development	22
Design and engineering of artificial microbial consortia for biohydrogen production	22
Synthetic molecular evolution of antimicrobial peptides	22
Can recombinant milk proteins replace those produced by animals?	22
Dissecting postharvest chilling injury through biotechnology	21
Metabolic regulation of epigenetic remodeling in immune cells	21
Engineering microbial diagnostics and therapeutics with smart control	21
Transient electronics: new opportunities for implantable neurotechnology	21
Engineering biology approaches for food and nutrient production by cyanobacteria	21
CRISPR-based metabolic engineering in non-model microorganisms	21
Engineered bacterial microcompartments: apps for programming metabolism	21
Engineering the plant microbiota in the context of the theory of ecological communities	21
SpaceHort: redesigning plants to support space exploration and on-earth sustainability	21
New synthetic biology tools for metabolic control	21
Phage Biobank: Present Challenges and Future Perspectives	21
Genetically encoded elastin-like polypeptide nanoparticles for drug delivery	21
Biosynthesis of human milk oligosaccharides via metabolic engineering approaches: current advances and challenges	21