Protein Expression and Purification

Article	Citations
Highly efficient overexpression and purification of multisubunit tethering complexes in Saccharomyces cerevisiae	41
Generation and characterization of a humanized anti-IL-17A rabbit monoclonal antibody	41
Efficient expression and purification of tag-free recombinant human procalcitonin (hPCT) with precise sequence in E. coli	29
A simple freeze-thaw based method for efficient purification of recombinant human proinsulin from inclusion bodies	28
Protein inclusion into ice can dissociate subunits	25
The heat-labile toxin B subunit of E. coli fused with VP6 from GCRV (Grass carp reovirus) was expressed and folded into an active protein in rice calli	24
Alocasia macrorrhiza rhizome lectin inhibits growth of pathogenic bacteria and human lung cancer cell in vitro and Ehrlich ascites carcinoma cell in vivo in mice	22
Optimization of the expression of the main protease from SARS-CoV-2	20
Optimization of the UDP-Xyl biocatalytic synthesis from Crassostrea gigas by orthogonal design method	20
Protein expression and purification, molecular interaction, and X-ray crystallographic analysis of baculovirus protein PK2	18
Expression, purification and characterization of SARS-CoV-2 spike RBD in ExpiCHO cells	17
Screening, expression and anti-tumor functional identification of anti-LAG-3 nanobodies	17
SEC-HPLC analysis of column load and flow-through provides critical understanding of low Protein A step yield	16
Optimized vector for functional expression of the human bitter taste receptor TAS2R14 in HEK293 cells	16
Isolation, expression, and characterization of potato (Solanum tuberosum) GH family 17 β-1,3-glucanase (Stglu) for exploring its potential as an antifungal agent	16
Editorial Board	15
Editorial Board	15
Simplified purification process of AsnB3 deamidated insulin variant and its comparison with human insulin	14
Purification and characterization of the intrinsically disordered Arabidopsis thaliana protein SOG1	14
Preparation and characterization of LGR5 LOOP region-specific nanobodies	14
Assessing four subdomain-specific affinity resins’ capability to separate half-antibody from intact bispecific antibody	12
Establishment of purification method for prokaryotic expression of Serpin gene for Dermatophagoides farinae	11
Expression and characterization of a lipase EstA from Bacillus subtilis KM-BS for application in bio-hydrolysis of waste cooking oil	11
The N-terminal polypeptide of a new shell matrix protein hicraqin accelerates the rate of calcium carbonate deposition	11
High-yield and cost-effective biosynthesis process for producing antimicrobial peptide AA139	11

Evaluation of the paired-Cas9 nickase and RNA-guided FokI genome editing tools in precise integration of an anti-CD52 bicistronic monoclonal antibody expression construct at Chinese hamster ovary cell	11
Highly efficient protein expression of Plasmodium vivax surface antigen, Pvs25, by silkworm and its biochemical analysis	11
Production and compositional analysis of full-length influenza virus hemagglutinin in Nanodiscs: Insights from multi-angle light scattering	10
Production of VP3-only virus-like particles of Adeno-associated virus 2 in E. coli cells	10
Expression, purification, and microscopic characterization of the tumor suppressor KLHL6	10
Improvement of thermostability of cholesterol oxidase from streptomyces Sp. SA-COO by random mutagenesis	10
Optimization of genetic code expansion in the baculovirus expression vector system (BEVS)	10
Polycistronic baculovirus expression of SUGT1 enables high-yield production of recombinant leucine-rich repeat proteins and protein complexes	10
The periplasmic expression and purification of AA15 lytic polysaccharide monooxygenases from insect species in Escherichia coli	9
Separation and identification of ACE inhibitory peptides from lizard fish proteins hydrolysates by metal affinity-immobilized magnetic liposome	8
Production of recombinant human ameloblastin by a fully native purification pathway	8
Molecular chaperone GroEL-GroES enhances the soluble expression of biologically active ovine growth hormone in the prokaryotic system	8
Validation of cold shock-based technique for purification of recombinant C. elegans Phosphodiesterase 3 protein expressed in E. coli	8
High performance production process development and scale-up of an anti-TSLP nanobody	8
Development of an effective method for purifying trypsin using a recombinant inhibitor	8
Molecular cloning and functional analysis of a destabilase from Hirudinaria manillensis	8
Editorial Board	8
One-step metal affinity purification of recombinant hFGF19 without using tags	8
Strategies for rapid production of crystallization quality coatomer WD40 domains	8
Optimized high-yield preparation of alkaline-solubilizable crystalline inclusion of the Bacillus thuringiensis Cry4Aa δ-endotoxin expressed in Escherichia coli	8
Efficient heterologous expression of cellobiose 2-epimerase gene in Escherichia coli under the control of T7 lac promoter without addition of IPTG and lactose	8
Overproduction, purification, and transcriptional activity of recombinant Acinetobacter baylyi ADP1 RNA polymerase holoenzyme	7
Purification and characterization of recombinant human superoxide dismutase integrated with resilin-like polypeptide	7
Expression, characterization and anti-colon cancer activity of recombinant ginseng peptides with amino acid tandem repeats	7
Multiple optimizations of recombinant plasmid for improving expression of Hepatitis B core antigen in Escherichia coli	7
An important consideration when expressing mAbs in Escherichia coli	7
Identification of chitinase from Bacillus velezensis strain S161 and its antifungal activity against Penicillium digitatum	7
A rapid procedure to generate stably transfected HEK293 suspension cells for recombinant protein manufacturing: Yield improvements, bioreactor production and downstream processing	7
Expression and purification of Drosophila OBP44a with the aids of LC-MS and NMR	7
Advantage of leaky expression, acid solubilization and CHAPS in the production of cost-effective bone morphogenetic Protein-2	7
Biochemical characterization of a novel bifunctional alginate lyase from Microbulbifer arenaceous	7
Bacterial expression, purification and characterization of the human Dectin-1 lectin domain for structural and functional studies	7
Solvent extraction of recombinant interferon alpha-2b from inclusion bodies and efficient refolding at high protein concentrations	7
High yield expression and purification of full-length Neurotensin with pyroglutamate modification	6
The construction of long-acting exendin-4 analog and its hypoglycemic effect in diabetic mice	6
Dual display hemagglutinin 1 and 5 on the surface of enveloped virus-like particles in silkworm expression system	6
Soluble expression of hMYDGF was improved by strain engineering and optimizations of fermentation strategies in Escherichia coli	6
Effective expression and characterization of the receptor binding domains in SARS-CoV-2 Spike proteins from original strain and variants of concern using Bombyx mori nucleopolyhedrovirus in silkworm	6
Unlocking the potential of Extensin Signal peptide and Elastin-like polypeptide tag fused to Shigella dysenteriae's IpaDSTxB to improve protein expression and purification in Nicotiana tabacum and Med	6
Escherichia coli as a versatile cell factory: Advances and challenges in recombinant protein production	6
Differentially labeled flaviviral protease-cofactor complex for NMR spectroscopic applications	6
Expression and purification of a novel single-chain diabody (scDb-hERG1/β1) from Pichia pastoris transformants	6
Facile purification of active recombinant mouse cytosolic carboxypeptidase 6 from Escherichia coli	6
A multidomain PARP14 construct suitable for bacterial expression	6
Zymography assisted quick purification, characterization and inhibition analysis of K. pneumoniae alkaline phosphatase by mercury and thiohydroxyal compounds	6
Corrigendum to “High-affinity nanobodies targeting IL-12B for the detection of fluorescence resonance energy transfer” [Protein Express. Purificat. 229 (2025) 106681]	6
Expression, purification, characterization and crystallization of Panax quinquefolius ginsenoside glycosyltransferase Pq3-O-UGT2	6
Deepening in the understanding of the role of collagen subunits on the differential molecular arrangement of P. glauca and M. merluccius marine collagens	6
Development of a bispecific Nanobody anti-F17 fimbria as a potential therapeutic tool	6
A protocol for production of perdeuterated OmpF porin for neutron crystallography	6

Editorial Board	6
Editorial Board	6
Recombinant human FOXJ1 protein binds DNA, forms higher-order oligomers, has gel-shifting domains and contains intrinsically disordered regions	6
Expression of recombinant swine ferritin heavy chain with enhanced solubility in Escherichia coli and simplified purification of ferritin nanoparticles	6
Navigating the Frontier: Advances in monoclonal antibody purity control	6
Combining the advantages of prokaryotic expression and T7 phage display systems to obtain antigens for antibody preparation	6
Preparation of recombinant neuritin protein	5
Soluble expression and purification of recombinant bovine ferritin H-chain	5
Improved production of class I phosphatidylinositol 4,5-bisphosphate 3-kinase	5
A streamlined, automated workflow to screen and triage large numbers of baculoviruses for protein expression	5
Uncovering the role of leucine 59 in Renilla luciferase stability and activity with error-prone PCR: Implications for protein engineering	5
Design, synthesis and application of near-infrared fluorescence probe IR-780-Crizotinib in detection of ALK positive tumors	5
CUREs for high-level Galectin-3 expression	5
Affinity gel synthesis from the p-aminobenzoic acid derivative 4-amino-2-methylbenzoic acid and purification of polyphenol oxidase from various plant sources	5
High level expression and purification of recombinant 3ABC non-structural protein of foot-and-mouth disease virus using SUMO fusion system	5
Characterization of two 3-deoxy-d-Arabino-Heptulosonate 7-phosphate synthases from Bacillus methanolicus	5
A procedure for producing an anti-AXL nanobody in E. coli	5
Analysis of the in vitro function and internalization ability of a humanized EGFR antibody AE01 expressed by Chinese hamster ovary cells	5
Purification of recombinantly produced somatostatin-28 comparing hydrochloric acid and polyethyleneimine as E. coli extraction aids	5
Optimized expression and purification of a human adenosine deaminase in E. coli and characterization of its Asp8Asn variant	5
Metal binding feature of copper‒induced metallothionein from freshwater crab Sinopotamon henanense reveals its Cu‒thionein character	5
Purification and characterization of an asialofetuin specific lectin from the rhizome of Xanthosoma violaceum Schott	5
Construction of a novel TROP2/CD3 bispecific antibody with potent antitumor activity and reduced induction of Th1 cytokines	5
Mini review for niche downstream processes	5
Heterologous expression, purification, and partial characterisation of the apicoplast protein 3-oxoacyl-[acyl-carrier-protein] reductase from Toxoplasma gondii	5
Synthesis of BLF1-containing trimethyl chitosan nanoparticles and evaluation of its immunogenicity and protection in syrian mice by oral and subcutaneous injections	5
Preparation and characterization of a novel humanized collagen III with repeated fragments of Gly300-Asp329	5
Editorial Board	5
Mass production and characterization of an endoglucanase from Coleoptera insect (Monochamus saltuarius) in yeast Kluyveromyces lactis	4
Recombinant organophosphorus hydrolase (OPH) expression in E. coli for the effective detection of organophosphate pesticides	4
A new trypsin inhibitor from Centrosema plumieri effective against digestive proteases from Tribolium castaneum, an eco-friendly alternative	4
Unravelling the reason for different binding behaviors exhibited by antibody aggregates towards preparative and analytical Protein A columns	4
Kinetic modeling: A tool for temperature shift and feeding optimization in cell culture process development	4
E. coli chromosomal-driven expression of NADK2 from A. thaliana: A preferable alternative to plasmid-driven expression for challenging proteins	4
Fast IMAC purification of non-tagged S100A8/A9 (calprotectin) from Homo sapiens and Sus scrofa	4
Bacterially expressed full length Hemagglutinin of Avian Influenza Virus H5N1 forms oligomers and exhibits hemagglutination	4
A novel method for synthesizing authentic SARS-CoV-2 main protease	4
Time- and cost-efficient bacterial expression and purification of potato apyrase	4
Editorial Board	4
Refolding, purification, and characterization of constitutive-active human-Smad8 produced as inclusion bodies in ClearColi® BL21 (DE3)	4
A comprehensive method for modeling and simulating ion exchange chromatography of complex mixtures	4
Partition coefficient screening – An effective approach for finding the best conditions for byproduct removal as demonstrated by a bispecific antibody purification case	4
A new combination approach to extracellular production of 5-aminolevulinic acid for purification and application in alleviating cadmium-induced oxidative stress in maize	4
Expression, purification and characterization of the transcription termination factor Rho from Azospirillum brasilense	4
High-throughput strategy for identification of Mycobacterium tuberculosis membrane protein expression conditions using folding reporter GFP	4
Recombinant Cas9 protein production in an endotoxin-free system and evaluation with editing the BCL11A gene in human cells	4
Functional expression of the chimera proteins of Nav1.7 and NavAb in Escherichia coli	4
Expression, purification and structural characterization of resveratrol synthase from Polygonum cuspidatum	4
Separation of native and C106-oxidized DJ-1 proteins by using column chromatography	4
Soluble expression of recombinant human interleukin-2 in Escherichia coli and its facile production	4
Preparation and characterization of nanobodies targeting SARS-CoV-2 papain-like protease	4
Antibody-mimetic drug conjugate with efficient internalization activity using anti-HER2 VHH and duocarmycin	4
Identification of host cell proteins that interact with the M protein of Pigeon paramyxovirus type 1	4
Bioinformatics analysis and polyclonal antibody preparation of Mesocricetus auratus DHX30	4
Enhanced production of functional CRISPR-AsCas12a protein in Escherichia coli	4
Development of efficient modules for recombinant protein expression and periplasmic localisation in Pseudomonas bharatica CSV86T	4
A recombinant human SLPI variant suppresses the formation of neutrophil extracellular traps at low concentrations in vitro	4
Hyper-expression of GFP-fused active hFGF21 in tobacco chloroplasts	4
Characterization of a halotolerant GH2 family β-galactosidase GalM from Microvirga sp. strain MC18	4
Sodium caprylate wash during Protein A chromatography as an effective means for removing protease(s) responsible for target antibody fragmentation	4
Purification and characterization of an amyloidogenic repeat domain from the functional amyloid Pmel17	4
Fusion with CTP increases the stability of recombinant neuritin	4
A simple method to purify intrinsically disordered proteins by adjusting trichloroacetic acid concentration	4
IgG-like bispecific antibody platforms with built-in purification-facilitating elements	4
Engineering an optimized expression operating unit for improved recombinant protein production in Escherichia coli	4
Milligram scale expression, refolding, and purification of Bombyx mori cocoonase using a recombinant E. coli system	4
Evaluation of BVDV E2 proteins based on recombinant baculovirus expression system production as diagnostic antigens and immunogens	4
Purification and comparison of soluble and membrane-bound polyphenol oxidase from potato (Solanum tuberosum) tubers	4
Expression and purification of human interferon alpha 2a (IFNα2a) in the methylotrophic yeast Pichia pastoris	3
Overcoming fluorescence loss in mEOS-based AAA+ unfoldase reporters through covalent linkage	3
Editorial Board	3
Biochemical characterization of a high affinity phosphate transporter (PiPT) from root endophyte fungus Piriformospora indica	3
Editorial Board	3
Design and construction of a phage-displayed Camelid nanobody library using a simple bioinformatics method	3
Molecular cloning, expression, and functional analysis of copper amine oxidase gene from mulberry (Morus alba L.)	3
Editorial Board	3
Characterization of recombinant E. coli expressing a novel fucosidase from Bacillus cereus 2–8 belonging to GH95 family	3
Efficient production of recombinant human FVII in CHO cells using the piggyBac transposon system	3
Isolation, purification and characterization of a novel esterase from camel rumen metagenome	3
Enzymatic characterization and thermostability improvement of an acidophilic endoxylanase PphXyn11 from Paenibacillus physcomitrellae XB	3

Expression, purification and preliminary crystallographic analysis of bacterial transmembrane protein EfeU for iron import	3
Generation and characterization of a monoclonal antibody against FGFR3 that protects mice from BoNT/A	3
Column-free purification of an artificial protein nanocage, TIP60	3
Novel expression of coat proteins from thermophilic bacteriophage ΦIN93 and evaluation for assembly into virus-like particles	3
Prokaryotic expression of soluble IFN-λ1 recombinant protein with cold-shock system	3
CHO cathepsin B identified as the protease responsible for a target bispecific antibody fragmentation	3
Corrigendum to “Expression and characterization of a novel β-1,4-endoglucanase from Bacillus subtilis strain isolated from a pulp and paper mill wastewater” [J. Protein Expr. Purif., 220 (2024) 106490	3
Efficient development of nanobody-based affinity chromatography for AAV8 purification	3
Recombinant highly antigenic truncated fusion-based protein as a diagnostic antigen for anti-SARS-CoV-2 nucleocapsid antibody ELISA	3
Recombinant production of antibody antigen-binding fragments with an N-terminal human growth hormone tag in mammalian cells	3
Recombinant expression and surface display of a zearalenone lactonohydrolase from Trichoderma aggressivum in Escherichia coli	3
Purification and anticancer activity of glutaminase and urease free intracellular l-asparaginase from Chaetomium sp.	3
Magnetically purification/immobilization of poly histidine-tagged proteins by PEGylated magnetic graphene oxide nanocomposites	3
Use of the redox-dependent intein system for enhancing production of the cyclic green fluorescent protein	3
Editorial Board	3
StxB fusion strategy for immunogenic enhancement: Recombinant expression of AcAMP antimicrobial peptide	3
Effects of removing a highly conserved disulfide bond in ubiquitin-associated domain of human HOIP on biochemical characteristics	3
Expression and characterization of a novel β-1,4-endoglucanase from Bacillus subtilis strain isolated from a pulp and paper mill wastewater	3
Enhanced expression and solubility of main protease (Mpro) of SARS-CoV-2 from E. coli	3
Engineering and characterization of GFP-targeting nanobody: Expression, purification, and post-translational modification analysis	3
Cloning and characterization of a hyaluronate lyase EsHyl8 from Escherichia sp. A99	3
Heterologous expression of a novel galactose-1-phosphate uridylyltransferase from Thermodesulfatator indicus and its application for bioproduction of Gal-β-1,4-GlcNAc-X	3
Editorial Board	3
Bioindustrial applications of thermostable Endoglucanase purified from Trichoderma viride towards the conversion of agrowastes to value-added products	3
High soluble expression and characterization of human GalNAc transferase T2 and T11 in Escherichia coli	3
Tetrahymena thermophila granule lattice protein 3 improves solubility of sexual stage malaria antigens expressed in Escherichia coli	3
Purification and characterization of eukaryotic ATP-dependent transporters homologously expressed in Pichia pastoris for structural studies by cryo-electron microscopy	3
Overexpression and truncation of a novel cold-adapted lipase with improved enzymatic characteristics	3
Cloning and characterization of a novel DNase gene from Trichogramma pretiosum	3
Heterologous expression of recombinant nattokinase in Escherichia coli BL21(DE3) and media optimization for overproduction of nattokinase using RSM	3
The combined effect of the gene copy number and chaperone overexpression on the recombinant bovine chymosin production in Pichia pastoris, with mutant ADH2 promoter	3
Biochemical and structural characterization of a novel L-isoleucine-4-dioxygenase (RaIDO) from Rahnella aquatilis	3
Enhancing the expression of multi-antigen chimeric TGAGS/BST protein from Toxoplasma gondii in Escherichia coli BL 21 Star during batch cultivation	3
Expression and purification of a cleavable recombinant fortilin from Escherichia coli for structure activity studies	3
Production of enzymes for the removal of odorous substances in plant biomass	3
Preparation and characterization of a high-affinity monoclonal antibody against nerve growth factor	3
Recombinant expression, purification, and characterization of full-length human BST-2 from Escherichia coli	3
Expression and biochemical characterization of a Bacillus subtilis catalase in Pichia pastoris X-33	3
Optimization of the heterologous expression and purification of Plasmodium falciparum generative cell specific 1 in Escherichia coli	3
Biochemical characterization and antifungal activity of a recombinant β-1,3-glucanase FlGluA from Flavobacterium sp. NAU1659	3
Expression and Characterization of the Staphylococcus aureus RecA protein: A mapping of canonical functions	3
Production of recombinant human proliferating cellular nuclear antigen (PCNA) for structural and biophysical characterization	3
Editorial Board	3
Optimized isolation of enzymatically active ubiquitin E3 ligase E6AP/UBE3A from mammalian cells	3
Recombinant expression, purification, and structural analysis of two ectodomains of Syndecan-1	3
Recombinant expression and functional characterization of FadD2 protein in Mycobacterium tuberculosis	2
Improved self-cleaving precipitation tags for efficient column free bioseparations	2
Editorial Board	2
Production and functional analysis of a phage displayed scFv recombinant antibody targeting EGFR/HER2 dimerization domain	2
Cloning and characterization of a novel nitric oxide synthase gene from Exiguobacterium profundum and its expression in Escherichia coli	2
Purification and characterization of full-length monomeric TEC family kinase, ITK	2
Optimized extraction, identification and characterization of the mosquitocidal surface layer protein from a local bacterial isolate Lysinibacillus sphaericus Q001	2
Expression, purification and crystallization of TGW6, which limits grain weight in rice	2
Improved expression and purification of highly-active 3 chymotrypsin-like protease from SARS-CoV-2	2
Removal of signal peptide variants by cation exchange chromatography: A case study	2
Cell-free protein production of a gamma secretase homolog	2
The choice of chromatographic resin for the purification of recombinant lysostaphin affects its activity	2
Functional characterization and structural insights of three cutinases from the ascomycete Fusarium verticillioides	2
Expression, purification and X-ray crystal diffraction analysis of alcohol dehydrogenase 1 from Artemisia annua L.	2
Screening and functional characterization of nanobodies targeting the transferrin receptor	2
Effect of cell-permeable grouper Manganese Superoxide Dismutase on environmental stress in fish	2
Viral removal by column chromatography in downstream processing of monoclonal antibodies	2
Editorial Board	2
Immunoglobulin-binding protein-based affinity chromatography in bispecific antibody purification: Functions beyond product capture	2
Cell free protein synthesis versus yeast expression – A comparison using insulin as a model protein	2
Editorial Board	2
Electroporation of SUMO-His-Cre protein triggers a specific recombinase-mediated cassette exchange in HEK 293T cells	2
Trehalose-6-phosphate phosphatase expression and enzymatic properties of Fusarium graminearum	2
STEP® vectors for rapid generation of stable transfected CHO cell pools and clones with high expression levels and product quality homogeneity of difficult-to-express proteins	2
A two-step method preparation of semaglutide through solid-phase synthesis and inclusion body expression	2
Apolipoprotein E4 heterologous expression, purification under non-denaturing conditions, and effects on neuronal clonal cell lines	2
Rational designation and characterization of a novel humanized collagen capable of self-assembling into triple helix and fibrils with D-period	2
Biochemical characterization of LysVpKK5 endolysin from a marine vibriophage	2
Editorial Board	2
Generation and characterization of a monoclonal antibody that binds to Galectin-1	2
Investigation and development of transient production process for porcine circovirus Type-2 (PCV2) capsid protein in HEK293F cells	2
Identification and characterization of a hydrophobin Vmh3 from Pleurotus ostreatus	2
Heterologous expression and purification of glutamate decarboxylase-1 from the model plant Arabidopsis thaliana: Characterization of the enzyme's in vitro truncation by thiol endopeptidase activity	2
Evaluate the potential use of TonB-dependent receptor protein as a subunit vaccine against Aeromonas veronii infection in Nile tilapia (Oreochromis niloticus)	2
Light-induced expression of a novel marine laccase in Escherichia coli from Marinomonas profundimaris and its application in synthetic dye decolorization	2
Cell-free protein synthesis of CD1E and B2M protein and in vitro interaction	2
Monitoring of the disulfide scrambled species by mixed-mode SEC-HPLC during the purification of a bispecific antibody	2
Editorial Board	2
Scalable purification of recombinant structural proteins, hagfish intermediate filament α and ɣ, from inclusion bodies for fiber formation	2
Resin and loading condition screening for effective and robust byproducts removal by anion exchange chromatography: A case study	2
Discovery and biochemical characterization of two hexokinases from Crassostrea gigas	2
Expression and purification of a recombinant ELRL-MAP30 with dual-targeting anti-tumor bioactivity	2
Nature of recombinant human serum amyloid A1 in Escherichia coli and its preferable approach for purification	2
Biochemical and biophysical characterization of the RAS family small GTPase protein DiRAS3	2
Characterization of a bifunctional and endolytic alginate lyase from Microbulbifer sp. ALW1 and its application in alginate oligosaccharides production from Laminaria japonica	2
Editorial Board	2
Heterologous expression, purification and single step efficient refolding of recombinant tissue plasminogen activator (Reteplase) from E. coli	2
Simple purification and characterization of soluble and homogenous ABC-F translation factors from Enterococcus faecium	2
Heterologous expression and biochemical comparison of two homologous SoxX proteins of endosymbiotic Candidatus Vesicomyosocius okutanii and free-living Hydrogenovibrio crunogenus from deep-sea environ	2
Editorial Board	2