OOIR: Observatory of International Research

Papers

(The TQCC of Protein Expression and Purification is 4. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-11-01 to 2025-11-01.)

Article	Citations
A simple freeze-thaw based method for efficient purification of recombinant human proinsulin from inclusion bodies	46
Protein inclusion into ice can dissociate subunits	44
Highly efficient overexpression and purification of multisubunit tethering complexes in Saccharomyces cerevisiae	42
Optimization of the expression of the main protease from SARS-CoV-2	27
The heat-labile toxin B subunit of E. coli fused with VP6 from GCRV (Grass carp reovirus) was expressed and folded into an active protein in rice calli	26
Optimization of the UDP-Xyl biocatalytic synthesis from Crassostrea gigas by orthogonal design method	23
Efficient expression and purification of tag-free recombinant human procalcitonin (hPCT) with precise sequence in E. coli	19
Generation and characterization of a humanized anti-IL-17A rabbit monoclonal antibody	18
Protein expression and purification, molecular interaction, and X-ray crystallographic analysis of baculovirus protein PK2	18
Expression, purification and characterization of SARS-CoV-2 spike RBD in ExpiCHO cells	18
SEC-HPLC analysis of column load and flow-through provides critical understanding of low Protein A step yield	17
Screening, expression and anti-tumor functional identification of anti-LAG-3 nanobodies	17
Alocasia macrorrhiza rhizome lectin inhibits growth of pathogenic bacteria and human lung cancer cell in vitro and Ehrlich ascites carcinoma cell in vivo in mice	17
Editorial Board	14
Isolation, expression, and characterization of potato (Solanum tuberosum) GH family 17 β-1,3-glucanase (Stglu) for exploring its potential as an antifungal agent	14
Optimized vector for functional expression of the human bitter taste receptor TAS2R14 in HEK293 cells	13
Production of VP3-only virus-like particles of Adeno-associated virus 2 in E. coli cells	13
Simplified purification process of AsnB3 deamidated insulin variant and its comparison with human insulin	12
Preparation and characterization of LGR5 LOOP region-specific nanobodies	12
Improvement of thermostability of cholesterol oxidase from streptomyces Sp. SA-COO by random mutagenesis	11
Purification of the HBV middle protein by two simple steps, application of ion exchange chromatography to improve protein homogeneity	11
The N-terminal polypeptide of a new shell matrix protein hicraqin accelerates the rate of calcium carbonate deposition	11
Purification and characterization of the intrinsically disordered Arabidopsis thaliana protein SOG1	11
Production and compositional analysis of full-length influenza virus hemagglutinin in Nanodiscs: Insights from multi-angle light scattering	10
High performance production process development and scale-up of an anti-TSLP nanobody	10

Assessing four subdomain-specific affinity resins’ capability to separate half-antibody from intact bispecific antibody	10
Evaluation of the paired-Cas9 nickase and RNA-guided FokI genome editing tools in precise integration of an anti-CD52 bicistronic monoclonal antibody expression construct at Chinese hamster ovary cell	10
The periplasmic expression and purification of AA15 lytic polysaccharide monooxygenases from insect species in Escherichia coli	10
Optimization of genetic code expansion in the baculovirus expression vector system (BEVS)	9
Editorial Board	9
Highly efficient protein expression of Plasmodium vivax surface antigen, Pvs25, by silkworm and its biochemical analysis	9
Polycistronic baculovirus expression of SUGT1 enables high-yield production of recombinant leucine-rich repeat proteins and protein complexes	9
High-yield and cost-effective biosynthesis process for producing antimicrobial peptide AA139	8
Development of an effective method for purifying trypsin using a recombinant inhibitor	8
Production of recombinant human ameloblastin by a fully native purification pathway	8
Molecular cloning and functional analysis of a destabilase from Hirudinaria manillensis	8
Expression, purification, and microscopic characterization of the tumor suppressor KLHL6	8
Establishment of purification method for prokaryotic expression of Serpin gene for Dermatophagoides farinae	8
Novel (R)-Hydroxynitrile lyase enzyme of Pyrus communis: Purification and characterization of its physicochemical and kinetic properties	8
Editorial Board	8
Expression and characterization of a lipase EstA from Bacillus subtilis KM-BS for application in bio-hydrolysis of waste cooking oil	8
Separation and identification of ACE inhibitory peptides from lizard fish proteins hydrolysates by metal affinity-immobilized magnetic liposome	8
Efficient heterologous expression of cellobiose 2-epimerase gene in Escherichia coli under the control of T7 lac promoter without addition of IPTG and lactose	8
Molecular chaperone GroEL-GroES enhances the soluble expression of biologically active ovine growth hormone in the prokaryotic system	8
Strategies for rapid production of crystallization quality coatomer WD40 domains	7
Bacterial expression, purification and characterization of the human Dectin-1 lectin domain for structural and functional studies	7
Overproduction, purification, and transcriptional activity of recombinant Acinetobacter baylyi ADP1 RNA polymerase holoenzyme	7
Optimized high-yield preparation of alkaline-solubilizable crystalline inclusion of the Bacillus thuringiensis Cry4Aa δ-endotoxin expressed in Escherichia coli	7
Purification and characterization of recombinant human superoxide dismutase integrated with resilin-like polypeptide	7
Dual display hemagglutinin 1 and 5 on the surface of enveloped virus-like particles in silkworm expression system	7
An important consideration when expressing mAbs in Escherichia coli	7
Expression, characterization and anti-colon cancer activity of recombinant ginseng peptides with amino acid tandem repeats	7
Advantage of leaky expression, acid solubilization and CHAPS in the production of cost-effective bone morphogenetic Protein-2	7
Expression and purification of Drosophila OBP44a with the aids of LC-MS and NMR	7
Identification of chitinase from Bacillus velezensis strain S161 and its antifungal activity against Penicillium digitatum	7
Facile purification of active recombinant mouse cytosolic carboxypeptidase 6 from Escherichia coli	7
One-step metal affinity purification of recombinant hFGF19 without using tags	7
Biochemical characterization of a novel bifunctional alginate lyase from Microbulbifer arenaceous	7
Multiple optimizations of recombinant plasmid for improving expression of Hepatitis B core antigen in Escherichia coli	7
Solvent extraction of recombinant interferon alpha-2b from inclusion bodies and efficient refolding at high protein concentrations	7
A rapid procedure to generate stably transfected HEK293 suspension cells for recombinant protein manufacturing: Yield improvements, bioreactor production and downstream processing	7
Corrigendum to “High-affinity nanobodies targeting IL-12B for the detection of fluorescence resonance energy transfer” [Protein Express. Purificat. 229 (2025) 106681]	6
The construction of long-acting exendin-4 analog and its hypoglycemic effect in diabetic mice	6
Soluble expression of hMYDGF was improved by strain engineering and optimizations of fermentation strategies in Escherichia coli	6
Development of a bispecific Nanobody anti-F17 fimbria as a potential therapeutic tool	6
Soluble expression and purification of recombinant bovine ferritin H-chain	6
Deepening in the understanding of the role of collagen subunits on the differential molecular arrangement of P. glauca and M. merluccius marine collagens	6
Navigating the Frontier: Advances in monoclonal antibody purity control	6
Expression, purification, characterization and crystallization of Panax quinquefolius ginsenoside glycosyltransferase Pq3-O-UGT2	6
Editorial Board	6
Recombinant human FOXJ1 protein binds DNA, forms higher-order oligomers, has gel-shifting domains and contains intrinsically disordered regions	6
Analysis of the in vitro function and internalization ability of a humanized EGFR antibody AE01 expressed by Chinese hamster ovary cells	6
Editorial Board	6
Optimized expression and purification of a human adenosine deaminase in E. coli and characterization of its Asp8Asn variant	6
Expression of recombinant swine ferritin heavy chain with enhanced solubility in Escherichia coli and simplified purification of ferritin nanoparticles	6

A multidomain PARP14 construct suitable for bacterial expression	6
Synthesis of BLF1-containing trimethyl chitosan nanoparticles and evaluation of its immunogenicity and protection in syrian mice by oral and subcutaneous injections	6
Effective expression and characterization of the receptor binding domains in SARS-CoV-2 Spike proteins from original strain and variants of concern using Bombyx mori nucleopolyhedrovirus in silkworm	6
A protocol for production of perdeuterated OmpF porin for neutron crystallography	6
Zymography assisted quick purification, characterization and inhibition analysis of K. pneumoniae alkaline phosphatase by mercury and thiohydroxyal compounds	6
Purification and characterization of an asialofetuin specific lectin from the rhizome of Xanthosoma violaceum Schott	5
Development of magnetic particle-based chemiluminescence immunoassay for quantitative determination of hepatitis C virus core antigen	5
High yield expression and purification of full-length Neurotensin with pyroglutamate modification	5
Enhanced production of functional CRISPR-AsCas12a protein in Escherichia coli	5
Affinity gel synthesis from the p-aminobenzoic acid derivative 4-amino-2-methylbenzoic acid and purification of polyphenol oxidase from various plant sources	5
Improved production of class I phosphatidylinositol 4,5-bisphosphate 3-kinase	5
Preparation of recombinant neuritin protein	5
Partition coefficient screening – An effective approach for finding the best conditions for byproduct removal as demonstrated by a bispecific antibody purification case	5
Heterologous expression, purification, and partial characterisation of the apicoplast protein 3-oxoacyl-[acyl-carrier-protein] reductase from Toxoplasma gondii	5
Design, synthesis and application of near-infrared fluorescence probe IR-780-Crizotinib in detection of ALK positive tumors	5
Preparation and characterization of a novel humanized collagen III with repeated fragments of Gly300-Asp329	5
Unlocking the potential of Extensin Signal peptide and Elastin-like polypeptide tag fused to Shigella dysenteriae's IpaDSTxB to improve protein expression and purification in Nicotiana tabacum and Med	5
A procedure for producing an anti-AXL nanobody in E. coli	5
Differentially labeled flaviviral protease-cofactor complex for NMR spectroscopic applications	5
Modular cell line for scalable and rapid in vitro evaluation of chimeric antigen receptors	5
Construction of a novel TROP2/CD3 bispecific antibody with potent antitumor activity and reduced induction of Th1 cytokines	5
A new combination approach to extracellular production of 5-aminolevulinic acid for purification and application in alleviating cadmium-induced oxidative stress in maize	5
A recombinant human SLPI variant suppresses the formation of neutrophil extracellular traps at low concentrations in vitro	5
Editorial Board	5
Uncovering the role of leucine 59 in Renilla luciferase stability and activity with error-prone PCR: Implications for protein engineering	5
Purification of recombinantly produced somatostatin-28 comparing hydrochloric acid and polyethyleneimine as E. coli extraction aids	5
Escherichia coli as a versatile cell factory: Advances and challenges in recombinant protein production	5
A streamlined, automated workflow to screen and triage large numbers of baculoviruses for protein expression	5
Editorial Board	5
A new trypsin inhibitor from Centrosema plumieri effective against digestive proteases from Tribolium castaneum, an eco-friendly alternative	5
CUREs for high-level Galectin-3 expression	5
Metal binding feature of copper‒induced metallothionein from freshwater crab Sinopotamon henanense reveals its Cu‒thionein character	5
Mini review for niche downstream processes	5
Characterization of two 3-deoxy-d-Arabino-Heptulosonate 7-phosphate synthases from Bacillus methanolicus	5
High level expression and purification of recombinant 3ABC non-structural protein of foot-and-mouth disease virus using SUMO fusion system	5
Hyper-expression of GFP-fused active hFGF21 in tobacco chloroplasts	4
Bioinformatics analysis and polyclonal antibody preparation of Mesocricetus auratus DHX30	4
Unravelling the reason for different binding behaviors exhibited by antibody aggregates towards preparative and analytical Protein A columns	4
Editorial Board	4
Biochemical characterization and antifungal activity of a recombinant β-1,3-glucanase FlGluA from Flavobacterium sp. NAU1659	4
Optimized isolation of enzymatically active ubiquitin E3 ligase E6AP/UBE3A from mammalian cells	4
High-throughput strategy for identification of Mycobacterium tuberculosis membrane protein expression conditions using folding reporter GFP	4
Purification and characterization of an amyloidogenic repeat domain from the functional amyloid Pmel17	4
Engineering an optimized expression operating unit for improved recombinant protein production in Escherichia coli	4
Kinetic modeling: A tool for temperature shift and feeding optimization in cell culture process development	4
Characterization of a halotolerant GH2 family β-galactosidase GalM from Microvirga sp. strain MC18	4
Separation of native and C106-oxidized DJ-1 proteins by using column chromatography	4
Evaluation of BVDV E2 proteins based on recombinant baculovirus expression system production as diagnostic antigens and immunogens	4
Time- and cost-efficient bacterial expression and purification of potato apyrase	4
Development of efficient modules for recombinant protein expression and periplasmic localisation in Pseudomonas bharatica CSV86T	4
Mass production and characterization of an endoglucanase from Coleoptera insect (Monochamus saltuarius) in yeast Kluyveromyces lactis	4
Purification and comparison of soluble and membrane-bound polyphenol oxidase from potato (Solanum tuberosum) tubers	4
Soluble expression of recombinant human interleukin-2 in Escherichia coli and its facile production	4
A novel method for synthesizing authentic SARS-CoV-2 main protease	4
High soluble expression and characterization of human GalNAc transferase T2 and T11 in Escherichia coli	4
Fast IMAC purification of non-tagged S100A8/A9 (calprotectin) from Homo sapiens and Sus scrofa	4
Recombinant Cas9 protein production in an endotoxin-free system and evaluation with editing the BCL11A gene in human cells	4
Editorial Board	4
Identification of host cell proteins that interact with the M protein of Pigeon paramyxovirus type 1	4
Expression, purification and structural characterization of resveratrol synthase from Polygonum cuspidatum	4
Expression, purification, and activity of sika deer prothymosin α protein	4
Fusion with CTP increases the stability of recombinant neuritin	4
IgG-like bispecific antibody platforms with built-in purification-facilitating elements	4
Antibody-mimetic drug conjugate with efficient internalization activity using anti-HER2 VHH and duocarmycin	4
A comprehensive method for modeling and simulating ion exchange chromatography of complex mixtures	4
Preparation and characterization of nanobodies targeting SARS-CoV-2 papain-like protease	4
Cloning and characterization of a hyaluronate lyase EsHyl8 from Escherichia sp. A99	4
Efficient development of nanobody-based affinity chromatography for AAV8 purification	4
Editorial Board	4
Column-free purification of an artificial protein nanocage, TIP60	4
E. coli chromosomal-driven expression of NADK2 from A. thaliana: A preferable alternative to plasmid-driven expression for challenging proteins	4
Expression, purification and characterization of the transcription termination factor Rho from Azospirillum brasilense	4
A simple method to purify intrinsically disordered proteins by adjusting trichloroacetic acid concentration	4
Bacterially expressed full length Hemagglutinin of Avian Influenza Virus H5N1 forms oligomers and exhibits hemagglutination	4
Functional expression of the chimera proteins of Nav1.7 and NavAb in Escherichia coli	4