OOIR: Observatory of International Research

Papers

(The TQCC of Protein Expression and Purification is 4. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-06-01 to 2025-06-01.)

Article	Citations
Highly efficient overexpression and purification of multisubunit tethering complexes in Saccharomyces cerevisiae	41
Generation and characterization of a humanized anti-IL-17A rabbit monoclonal antibody	39
Efficient expression and purification of tag-free recombinant human procalcitonin (hPCT) with precise sequence in E. coli	34
The heat-labile toxin B subunit of E. coli fused with VP6 from GCRV (Grass carp reovirus) was expressed and folded into an active protein in rice calli	27
A simple freeze-thaw based method for efficient purification of recombinant human proinsulin from inclusion bodies	26
Protein inclusion into ice can dissociate subunits	23
Optimization of the UDP-Xyl biocatalytic synthesis from Crassostrea gigas by orthogonal design method	21
Optimization of the expression of the main protease from SARS-CoV-2	21
Protein expression and purification, molecular interaction, and X-ray crystallographic analysis of baculovirus protein PK2	20
Screening, expression and anti-tumor functional identification of anti-LAG-3 nanobodies	20
Alocasia macrorrhiza rhizome lectin inhibits growth of pathogenic bacteria and human lung cancer cell in vitro and Ehrlich ascites carcinoma cell in vivo in mice	18
Expression, purification and characterization of SARS-CoV-2 spike RBD in ExpiCHO cells	17
SEC-HPLC analysis of column load and flow-through provides critical understanding of low Protein A step yield	17
Optimized vector for functional expression of the human bitter taste receptor TAS2R14 in HEK293 cells	16
Isolation, expression, and characterization of potato (Solanum tuberosum) GH family 17 β-1,3-glucanase (Stglu) for exploring its potential as an antifungal agent	16
Editorial Board	15
Simplified purification process of AsnB3 deamidated insulin variant and its comparison with human insulin	15
Editorial Board	15
Preparation and characterization of LGR5 LOOP region-specific nanobodies	15
Assessing four subdomain-specific affinity resins’ capability to separate half-antibody from intact bispecific antibody	14
Purification and characterization of an FeII- and α-ketoglutarate-dependent xanthine hydroxylase from Aspergillus oryzae	14
Purification and characterization of the intrinsically disordered Arabidopsis thaliana protein SOG1	14
Expression and characterization of a lipase EstA from Bacillus subtilis KM-BS for application in bio-hydrolysis of waste cooking oil	14
Recombinant expression, purification and characterization of human soluble tumor necrosis factor receptor 2	14
Highly efficient protein expression of Plasmodium vivax surface antigen, Pvs25, by silkworm and its biochemical analysis	11

Establishment of purification method for prokaryotic expression of Serpin gene for Dermatophagoides farinae	11
High-yield and cost-effective biosynthesis process for producing antimicrobial peptide AA139	11
Evaluation of the paired-Cas9 nickase and RNA-guided FokI genome editing tools in precise integration of an anti-CD52 bicistronic monoclonal antibody expression construct at Chinese hamster ovary cell	11
Improvement of thermostability of cholesterol oxidase from streptomyces Sp. SA-COO by random mutagenesis	11
Optimization of genetic code expansion in the baculovirus expression vector system (BEVS)	10
Production of VP3-only virus-like particles of Adeno-associated virus 2 in E. coli cells	10
Expression, purification, and microscopic characterization of the tumor suppressor KLHL6	10
Production and compositional analysis of full-length influenza virus hemagglutinin in Nanodiscs: Insights from multi-angle light scattering	10
Separation and identification of ACE inhibitory peptides from lizard fish proteins hydrolysates by metal affinity-immobilized magnetic liposome	10
The N-terminal polypeptide of a new shell matrix protein hicraqin accelerates the rate of calcium carbonate deposition	10
High performance production process development and scale-up of an anti-TSLP nanobody	9
Polycistronic baculovirus expression of SUGT1 enables high-yield production of recombinant leucine-rich repeat proteins and protein complexes	9
Strategies for rapid production of crystallization quality coatomer WD40 domains	9
The periplasmic expression and purification of AA15 lytic polysaccharide monooxygenases from insect species in Escherichia coli	9
Purification, secondary structure and antioxidant activity of metallothionein zinc-binding proteins from Arca subcrenata	9
Efficient heterologous expression of cellobiose 2-epimerase gene in Escherichia coli under the control of T7 lac promoter without addition of IPTG and lactose	8
Development of an effective method for purifying trypsin using a recombinant inhibitor	8
Solvent extraction of recombinant interferon alpha-2b from inclusion bodies and efficient refolding at high protein concentrations	8
Editorial Board	8
One-step metal affinity purification of recombinant hFGF19 without using tags	8
Molecular chaperone GroEL-GroES enhances the soluble expression of biologically active ovine growth hormone in the prokaryotic system	8
High-level extracellular production and immobilisation of methyl parathion hydrolase from Plesiomonas sp. M6 expressed in Pichia pastoris	8
Production of recombinant human ameloblastin by a fully native purification pathway	8
Editorial Board	7
Bacterial expression, purification and characterization of the human Dectin-1 lectin domain for structural and functional studies	7
Overproduction, purification, and transcriptional activity of recombinant Acinetobacter baylyi ADP1 RNA polymerase holoenzyme	7
Identification of chitinase from Bacillus velezensis strain S161 and its antifungal activity against Penicillium digitatum	7
Expression and purification of Drosophila OBP44a with the aids of LC-MS and NMR	7
The construction of long-acting exendin-4 analog and its hypoglycemic effect in diabetic mice	7
Advantage of leaky expression, acid solubilization and CHAPS in the production of cost-effective bone morphogenetic Protein-2	7
Facile purification of active recombinant mouse cytosolic carboxypeptidase 6 from Escherichia coli	7
A rapid procedure to generate stably transfected HEK293 suspension cells for recombinant protein manufacturing: Yield improvements, bioreactor production and downstream processing	7
Biochemical characterization of a novel bifunctional alginate lyase from Microbulbifer arenaceous	7
Dual display hemagglutinin 1 and 5 on the surface of enveloped virus-like particles in silkworm expression system	7
Editorial Board	7
Multiple optimizations of recombinant plasmid for improving expression of Hepatitis B core antigen in Escherichia coli	7
Optimized high-yield preparation of alkaline-solubilizable crystalline inclusion of the Bacillus thuringiensis Cry4Aa δ-endotoxin expressed in Escherichia coli	7
An important consideration when expressing mAbs in Escherichia coli	7
Purification and characterization of recombinant human superoxide dismutase integrated with resilin-like polypeptide	7
Expression, characterization and anti-colon cancer activity of recombinant ginseng peptides with amino acid tandem repeats	7
A multidomain PARP14 construct suitable for bacterial expression	6
High yield expression and purification of full-length Neurotensin with pyroglutamate modification	6
A protocol for production of perdeuterated OmpF porin for neutron crystallography	6
Effective expression and characterization of the receptor binding domains in SARS-CoV-2 Spike proteins from original strain and variants of concern using Bombyx mori nucleopolyhedrovirus in silkworm	6
Design, synthesis and application of near-infrared fluorescence probe IR-780-Crizotinib in detection of ALK positive tumors	6
Optimized expression and purification of a human adenosine deaminase in E. coli and characterization of its Asp8Asn variant	6
Differentially labeled flaviviral protease-cofactor complex for NMR spectroscopic applications	6
Unlocking the potential of Extensin Signal peptide and Elastin-like polypeptide tag fused to Shigella dysenteriae's IpaDSTxB to improve protein expression and purification in Nicotiana tabacum and Med	6
Soluble expression of hMYDGF was improved by strain engineering and optimizations of fermentation strategies in Escherichia coli	6
Expression and purification of a novel single-chain diabody (scDb-hERG1/β1) from Pichia pastoris transformants	6

Escherichia coli as a versatile cell factory: Advances and challenges in recombinant protein production	6
Soluble expression and purification of recombinant bovine ferritin H-chain	6
Recombinant human FOXJ1 protein binds DNA, forms higher-order oligomers, has gel-shifting domains and contains intrinsically disordered regions	6
Analysis of the in vitro function and internalization ability of a humanized EGFR antibody AE01 expressed by Chinese hamster ovary cells	6
Development of a bispecific Nanobody anti-F17 fimbria as a potential therapeutic tool	6
Deepening in the understanding of the role of collagen subunits on the differential molecular arrangement of P. glauca and M. merluccius marine collagens	6
Zymography assisted quick purification, characterization and inhibition analysis of K. pneumoniae alkaline phosphatase by mercury and thiohydroxyal compounds	6
Combining the advantages of prokaryotic expression and T7 phage display systems to obtain antigens for antibody preparation	6
Synthesis of BLF1-containing trimethyl chitosan nanoparticles and evaluation of its immunogenicity and protection in syrian mice by oral and subcutaneous injections	6
Expression, purification, characterization and crystallization of Panax quinquefolius ginsenoside glycosyltransferase Pq3-O-UGT2	6
CUREs for high-level Galectin-3 expression	5
Expression, purification and microscopic characterization of human ATP-binding cassette sub-family B member 7 protein	5
Improved production of class I phosphatidylinositol 4,5-bisphosphate 3-kinase	5
A new trypsin inhibitor from Centrosema plumieri effective against digestive proteases from Tribolium castaneum, an eco-friendly alternative	5
Development of efficient modules for recombinant protein expression and periplasmic localisation in Pseudomonas bharatica CSV86T	5
Metal binding feature of copper‒induced metallothionein from freshwater crab Sinopotamon henanense reveals its Cu‒thionein character	5
Affinity gel synthesis from the p-aminobenzoic acid derivative 4-amino-2-methylbenzoic acid and purification of polyphenol oxidase from various plant sources	5
High level expression and purification of recombinant 3ABC non-structural protein of foot-and-mouth disease virus using SUMO fusion system	5
Partition coefficient screening – An effective approach for finding the best conditions for byproduct removal as demonstrated by a bispecific antibody purification case	5
Construction of a novel TROP2/CD3 bispecific antibody with potent antitumor activity and reduced induction of Th1 cytokines	5
A procedure for producing an anti-AXL nanobody in E. coli	5
Heterologous expression, purification, and partial characterisation of the apicoplast protein 3-oxoacyl-[acyl-carrier-protein] reductase from Toxoplasma gondii	5
Recombinant organophosphorus hydrolase (OPH) expression in E. coli for the effective detection of organophosphate pesticides	5
Uncovering the role of leucine 59 in Renilla luciferase stability and activity with error-prone PCR: Implications for protein engineering	5
Preparation and characterization of a novel humanized collagen III with repeated fragments of Gly300-Asp329	5
Editorial Board	5
Characterization of two 3-deoxy-d-Arabino-Heptulosonate 7-phosphate synthases from Bacillus methanolicus	5
Purification and characterization of an asialofetuin specific lectin from the rhizome of Xanthosoma violaceum Schott	5
Mini review for niche downstream processes	5
A streamlined, automated workflow to screen and triage large numbers of baculoviruses for protein expression	5
Milligram scale expression, refolding, and purification of Bombyx mori cocoonase using a recombinant E. coli system	5
Editorial Board	5
Purification of recombinantly produced somatostatin-28 comparing hydrochloric acid and polyethyleneimine as E. coli extraction aids	5
Preparation of recombinant neuritin protein	5
Time- and cost-efficient bacterial expression and purification of potato apyrase	5
Hyper-expression of GFP-fused active hFGF21 in tobacco chloroplasts	4
Antibody-mimetic drug conjugate with efficient internalization activity using anti-HER2 VHH and duocarmycin	4
Soluble expression of recombinant human interleukin-2 in Escherichia coli and its facile production	4
Generation and characterization of a monoclonal antibody against FGFR3 that protects mice from BoNT/A	4
Sodium caprylate wash during Protein A chromatography as an effective means for removing protease(s) responsible for target antibody fragmentation	4
Optimization of the heterologous expression and purification of Plasmodium falciparum generative cell specific 1 in Escherichia coli	4
Expression, purification and structural characterization of resveratrol synthase from Polygonum cuspidatum	4
Expression, purification and characterization of the transcription termination factor Rho from Azospirillum brasilense	4
Fusion with CTP increases the stability of recombinant neuritin	4
Identification of host cell proteins that interact with the M protein of Pigeon paramyxovirus type 1	4
Separation of native and C106-oxidized DJ-1 proteins by using column chromatography	4
Refolding, purification, and characterization of constitutive-active human-Smad8 produced as inclusion bodies in ClearColi® BL21 (DE3)	4
Mass production and characterization of an endoglucanase from Coleoptera insect (Monochamus saltuarius) in yeast Kluyveromyces lactis	4
A novel method for synthesizing authentic SARS-CoV-2 main protease	4
High-throughput strategy for identification of Mycobacterium tuberculosis membrane protein expression conditions using folding reporter GFP	4
Editorial Board	4
Evaluation of BVDV E2 proteins based on recombinant baculovirus expression system production as diagnostic antigens and immunogens	4
Bacterially expressed full length Hemagglutinin of Avian Influenza Virus H5N1 forms oligomers and exhibits hemagglutination	4
Recombinant Cas9 protein production in an endotoxin-free system and evaluation with editing the BCL11A gene in human cells	4
A simple method to purify intrinsically disordered proteins by adjusting trichloroacetic acid concentration	4
Kinetic modeling: A tool for temperature shift and feeding optimization in cell culture process development	4
Functional expression of the chimera proteins of Nav1.7 and NavAb in Escherichia coli	4
IgG-like bispecific antibody platforms with built-in purification-facilitating elements	4
A comprehensive method for modeling and simulating ion exchange chromatography of complex mixtures	4
Preparation and characterization of nanobodies targeting SARS-CoV-2 papain-like protease	4
Characterization of a halotolerant GH2 family β-galactosidase GalM from Microvirga sp. strain MC18	4
Efficient development of nanobody-based affinity chromatography for AAV8 purification	4
Editorial Board	4
E. coli chromosomal-driven expression of NADK2 from A. thaliana: A preferable alternative to plasmid-driven expression for challenging proteins	4
Fast IMAC purification of non-tagged S100A8/A9 (calprotectin) from Homo sapiens and Sus scrofa	4
Optimization strategies for expression and purification of soluble N-terminal domain of human centriolar protein SAS-6 in Escherichia coli	4
Purification and characterization of an amyloidogenic repeat domain from the functional amyloid Pmel17	4
Engineering an optimized expression operating unit for improved recombinant protein production in Escherichia coli	4
Unravelling the reason for different binding behaviors exhibited by antibody aggregates towards preparative and analytical Protein A columns	4
Purification and comparison of soluble and membrane-bound polyphenol oxidase from potato (Solanum tuberosum) tubers	4