Protein Expression and Purification

Article	Citations
Development of a novel capture step for purification of antigen binding fragments (Fabs)	37
α-Conotoxin recombinant protein ImI-AFP3 efficiently inhibits the growth and migration of lung cancer cells	32
Expression of blue pigment synthetase a from Streptomyces lavenduale reveals insights on the effects of refactoring biosynthetic megasynthases for heterologous expression in Escherichia coli.	31
Editorial Board	26
SEC-HPLC analysis of column load and flow-through provides critical understanding of low Protein A step yield	23
Genetically encoded site-specific 19F unnatural amino acid incorporation in V. natriegens for in-cell NMR analysis	21
Purification and characterization of tomato arginine decarboxylase and its inhibition by the bacterial small molecule phevamine A	21
Efficient expression and purification of tag-free recombinant human procalcitonin (hPCT) with precise sequence in E. coli	20
Editorial Board	19
Editorial Board	19
Expression in Escherichia coli, purification and kinetic characterization of LAPLm, a Leishmania major M17-aminopeptidase	18
Evaluating a new mixed-mode resin Diamond MMC Mustang using Capto MMC ImpRes as a benchmark	17
Expression and purification of a cleavable recombinant fortilin from Escherichia coli for structure activity studies	16
Generation and characterization of a humanized anti-IL-17A rabbit monoclonal antibody	16
Protein expression and purification, molecular interaction, and X-ray crystallographic analysis of baculovirus protein PK2	15
Effects of removing a highly conserved disulfide bond in ubiquitin-associated domain of human HOIP on biochemical characteristics	15
Expression, purification, and study on the efficiency of a new potent recombinant scFv antibody against the SARS-CoV-2 spike RBD in E. coli BL21	15
Alocasia macrorrhiza rhizome lectin inhibits growth of pathogenic bacteria and human lung cancer cell in vitro and Ehrlich ascites carcinoma cell in vivo in mice	14
Two major mechanisms contributing to copurification of CHO host cell proteins and strategies to minimize their negative impact	14
Recombinant expression and surface display of a zearalenone lactonohydrolase from Trichoderma aggressivum in Escherichia coli	14
Screening, expression and anti-tumor functional identification of anti-LAG-3 nanobodies	13
Heterologous expression of a novel galactose-1-phosphate uridylyltransferase from Thermodesulfatator indicus and its application for bioproduction of Gal-β-1,4-GlcNAc-X	13
Isolation of recombinant apolipoprotein E4 N-terminal domain by foam fractionation	12
Enhanced expression and solubility of main protease (Mpro) of SARS-CoV-2 from E. coli	12
The calcium-binding photoprotein clytin II: Expression of the preferred human codon-optimized clytin II gene in Chinese hamster ovary-K1 cells and its use in the G-protein-coupled receptor assays	11

Characterization of a highly thermostable recombinant xylanase from Anoxybacillus ayderensis	10
Improving process robustness of cation exchange chromatography with cationic buffers for the reduction of aggregates	10
Rational design of a Nivolumab-based ANTI-PD-1 single chain variable fragment that blocks the interaction between PD-1 expressed on T-CELLS and PD-L1 ON CHO cells	10
Improved production of class I phosphatidylinositol 4,5-bisphosphate 3-kinase	10
Heterologous expression, purification, and partial characterisation of the apicoplast protein 3-oxoacyl-[acyl-carrier-protein] reductase from Toxoplasma gondii	10
Expression and purification of an NP-hoc fusion protein: Utilizing influenza a nucleoprotein and phage T4 hoc protein	9
Screening of six cation exchange resins for high binding capacity, monomer purity and step yield: A case study	9
Metal binding feature of copper‒induced metallothionein from freshwater crab Sinopotamon henanense reveals its Cu‒thionein character	9
Enzymatic and antibacterial activity of the recombinant endolysin PVP-SE1gp146 expressed in Hansenula polymorpha	9
A streamlined, automated workflow to screen and triage large numbers of baculoviruses for protein expression	8
The heat-labile toxin B subunit of E. coli fused with VP6 from GCRV (Grass carp reovirus) was expressed and folded into an active protein in rice calli	8
NADP+-dependent l-arginine dehydrogenase from Pseudomonas velonii: Purification, characterization and application to an l-arginine assay	8
Optimization of the expression of the main protease from SARS-CoV-2	8
Expression, purification, and refolding of the recombinant extracellular domain β1-subunit of the dog Na+/K+-ATPase of the epithelial cells	8
Editorial Board	8
Proteomic analysis reveals a role of ADP-glucose pyrophosphorylase in the asynchronous filling of rice superior and inferior spikelets	8
Chemometric modeling of the electrochemical data to investigate proline cis/trans isomeration effect on aggregation of Tau protein	8
Tick-borne encephalitis nonstructural protein NS1 expressed in E. coli retains immunological properties of the native protein	8
Biochemical and functional characterization of the N-terminal ubiquitin-like domain of human SHARPIN	8
Cytoplasmic production of Fabs in chemically defined media in fed-batch fermentation	8
Expression of HCV genotype-4 core antigen in prokaryotic E. coli system for diagnosis of HCV infection in Egypt	8
Novel expression of coat proteins from thermophilic bacteriophage ΦIN93 and evaluation for assembly into virus-like particles	7
Molecular cloning and biochemical characterization of a trehalose synthase from Myxococcus sp. strain V11	7
Identification and characterization of a novel sativene synthase from Fischerella thermalis	7
Enzymatic characterization and thermostability improvement of an acidophilic endoxylanase PphXyn11 from Paenibacillus physcomitrellae XB	7
Enhancing the thermostability of transglutaminase from Streptomyces mobaraensis based on the rational design of a disulfide bond	7
Highly efficient overexpression and purification of multisubunit tethering complexes in Saccharomyces cerevisiae	7
Expression and biochemical characterization of a Bacillus subtilis catalase in Pichia pastoris X-33	7
Expression, purification and characterization of SARS-CoV-2 spike RBD in ExpiCHO cells	7
Biochemical and structural characterization of a novel L-isoleucine-4-dioxygenase (RaIDO) from Rahnella aquatilis	7
Recombinant expression and molecular characterization of buffalo sperm lysozyme-like protein 1	7
Process development and characterization of recombinant nucleocapsid protein for its application on COVID-19 diagnosis	7
Purification, crystallization and preliminary crystallographic analysis of Chlamydophila pneumoniae AP endonuclease IV	7
Cloning and expression of Neurospora crassa cellobiohydrolase II in Pichia pastoris	7
CUREs for high-level Galectin-3 expression	6
Expression of the Fusarium graminearum galactose oxidase GaoA in Saccharomyces cerevisiae	6
Expression and characterization of 1,4-α-glucan branching enzyme from Microvirga sp. MC18 and its application in the preparation of slowly digestible starch	6
Characterization of two 3-deoxy-d-Arabino-Heptulosonate 7-phosphate synthases from Bacillus methanolicus	6
One-step purification of tag free and soluble lamin B1 from an E. coli bacterial expression system	6
Removal and monitoring of residual nucleic acids from core streptavidin inclusion bodies for increased refolding yield	6
A simple freeze-thaw based method for efficient purification of recombinant human proinsulin from inclusion bodies	6
Expression and purification of S5196-272 and S6200-317 proteins from Tilapia Lake Virus (TiLV) and their potential use as vaccines	6
Reconstitution of the full transmembrane cadherin-catenin complex	6
A simple method to purify recombinant HCV core protein expressed in Pichia pastoris for obtaining virus-like particles and producing monoclonal antibodies	6
A review of alternative promoters for optimal recombinant protein expression in baculovirus-infected insect cells	6
Design and construction of a phage-displayed Camelid nanobody library using a simple bioinformatics method	6
Sampling the cultivation parameter space for the bacterial production of TLR1 intracellular domain reveals the multiple optima	6
Purification and characterization of a novel extracellular, alkaline, thermoactive, and detergent-compatible lipase from Aeromonas caviae LipT51 for application in detergent industry	6
Expression, purification and characterization of the RhoA-binding domain of human SHIP2 in E.coli	6
Purification and HDL-like particle formation of apolipoprotein A-I after co-expression with the EDDIE mutant of Npro autoprotease	6

Plant cellulose synthase membrane protein isolation directly from Pichia pastoris protoplasts, liposome reconstitution, and its enzymatic characterization	5
Immobilization of fibrinolytic protease from Mucor subtilissimus UCP 1262 in magnetic nanoparticles	5
Optimization of the UDP-Xyl biocatalytic synthesis from Crassostrea gigas by orthogonal design method	5
Use of waste frying oil and coconut pulp for the production, isolation, and characterization of a new lipase from Moesziomyces aphidis	5
Optimized vector for functional expression of the human bitter taste receptor TAS2R14 in HEK293 cells	5
Expression, purification and microscopic characterization of human ATP-binding cassette sub-family B member 7 protein	5
Isolation, expression, and characterization of potato (Solanum tuberosum) GH family 17 β-1,3-glucanase (Stglu) for exploring its potential as an antifungal agent	5
Magnetically purification/immobilization of poly histidine-tagged proteins by PEGylated magnetic graphene oxide nanocomposites	5
Optimal secretion of thermostable Beta-glucosidase in Bacillus subtilis by signal peptide optimization	5
Editorial Board	5
Antibody mimetic drug conjugate manufactured by high-yield Escherichia coli expression and non-covalent binding system	5
Recombinant organophosphorus hydrolase (OPH) expression in E. coli for the effective detection of organophosphate pesticides	5
Linkers: A synergistic way for the synthesis of chimeric proteins	5
Editorial Board	5
Biochemical characterization of a high affinity phosphate transporter (PiPT) from root endophyte fungus Piriformospora indica	5
Recombinant highly antigenic truncated fusion-based protein as a diagnostic antigen for anti-SARS-CoV-2 nucleocapsid antibody ELISA	5
Preparation and characterization of a high-affinity monoclonal antibody against nerve growth factor	5
IgG-like bispecific antibody platforms with built-in purification-facilitating elements	5
Protein inclusion into ice can dissociate subunits	5
Biochemical characterization and redesign of the coenzyme specificity of a novel monofunctional NAD+-dependent homoserine dehydrogenase from the human pathogen Neisseria gonorrhoeae	5
CHO cathepsin B identified as the protease responsible for a target bispecific antibody fragmentation	5
Refolding of the Deinococcus Radiodurans phytochrome photosensory module and an extended backbone resonance assignment by solution NMR	5
Large scale production of synthetic spider silk proteins in Escherichia coli	5
Heterologous expression of recombinant nattokinase in Escherichia coli BL21(DE3) and media optimization for overproduction of nattokinase using RSM	5
Optimization strategies for expression of a novel bifunctional anti-PD-L1/TGFBR2-ECD fusion protein	5
Improved thermostability of D-allulose 3-epimerase from Clostridium bolteae ATCC BAA-613 by proline residue substitution	5
A new trypsin inhibitor from Centrosema plumieri effective against digestive proteases from Tribolium castaneum, an eco-friendly alternative	5
Partition coefficient screening – An effective approach for finding the best conditions for byproduct removal as demonstrated by a bispecific antibody purification case	5
A procedure for producing an anti-AXL nanobody in E. coli	5
Recombinant expression, purification and characterization of an active bacterial feruloyl-CoA synthase with potential for application in vanillin production	5
Editorial Board	4
Mass production and characterization of an endoglucanase from Coleoptera insect (Monochamus saltuarius) in yeast Kluyveromyces lactis	4
Synergetic interaction of capsid proteins for virus-like particles assembly of foot-and-mouth disease virus (serotype O) from the inclusion bodies	4
Editorial Board	4
Expression and purification of human interferon alpha 2a (IFNα2a) in the methylotrophic yeast Pichia pastoris	4
Complementary methods for monitoring hole-hole homodimer associated with a WuXiBody-based asymmetric bispecific antibody	4
Purification and characterization of eukaryotic ATP-dependent transporters homologously expressed in Pichia pastoris for structural studies by cryo-electron microscopy	4
Rapid purification of rabbit immunoglobulins using a single-step, negative-selection chromatography	4
Expression, purification, characterization, and patient IgE reactivity of new macadamia nut iso-allergen	4
An improved production and purification protocol for recombinant soluble human fibroblast activation protein alpha	4
The periplasmic expression and purification of AA15 lytic polysaccharide monooxygenases from insect species in Escherichia coli	4
Glutathione synthetase from Hydra vulgaris: Molecular cloning, overexpression, purification and partial characterization	4
Editorial Board	4
Recombinant expression of Yersinia ruckeri outer membrane proteins in Escherichia coli extracellular vesicles	4
Removing a light-chain missing byproduct by MMC ImpRes mixed-mode chromatography under weak partitioning mode in purifying a WuXiBody-based bispecific antibody	4
Discovery and characterization of hydroxylysine O-glycosylation in an engineered IL-2 fusion protein	4
Robust production of active Ulp1 (SUMO protease) from inclusion bodies	4
Expression, purification, and microscopic characterization of the tumor suppressor KLHL6	4
High yield expression in Pichia pastoris of human neutrophil elastase fused to cytochrome B5	4
Milligram scale expression, refolding, and purification of Bombyx mori cocoonase using a recombinant E. coli system	4
High performance production process development and scale-up of an anti-TSLP nanobody	4
Hyper-expression of GFP-fused active hFGF21 in tobacco chloroplasts	4
Editorial Board	4
Editorial Board	4
Editorial Board	4
A comprehensive method for modeling and simulating ion exchange chromatography of complex mixtures	4
Recombinant production of antibody antigen-binding fragments with an N-terminal human growth hormone tag in mammalian cells	4
Optimization of genetic code expansion in the baculovirus expression vector system (BEVS)	4
A generalizable protocol for expression and purification of membrane-bound bacterial phosphoglycosyl transferases in liponanoparticles	4
Efficient improvement of surface displayed lipase from Rhizomucor miehei in PichiaPink™ protease-deficient system	4
The N-terminal polypeptide of a new shell matrix protein hicraqin accelerates the rate of calcium carbonate deposition	3
Effect of Clp protease from Corynebacterium glutamicum on heterologous protein expression	3
Development of efficient modules for recombinant protein expression and periplasmic localisation in Pseudomonas bharatica CSV86T	3
Chagasin from Trypanosoma cruzi as a molecular scaffold to express epitopes of TSA-1 as soluble recombinant chimeras	3
Establishment of purification method for prokaryotic expression of Serpin gene for Dermatophagoides farinae	3
Purification and characterization of an FeII- and α-ketoglutarate-dependent xanthine hydroxylase from Aspergillus oryzae	3
A comparative study and evaluation of anti-EGFR nanobodies expressed in Pichia pastoris and Escherichia coli as antitumor moieties	3
MmpL3, the trehalose monomycolate transporter, is stable in solution in several detergents and can be reconstituted into peptidiscs	3
Overexpression and truncation of a novel cold-adapted lipase with improved enzymatic characteristics	3
Diverse approaches to isolate HLA class I molecules from bacterial inclusion bodies, forming heterotrimeric complexes	3
Assessing four subdomain-specific affinity resins’ capability to separate half-antibody from intact bispecific antibody	3
Expression of soluble recombinant human matrix metalloproteinase 9 and generation of its monoclonal antibody	3
High-yield and cost-effective biosynthesis process for producing antimicrobial peptide AA139	3
Expression and characterization of a lipase EstA from Bacillus subtilis KM-BS for application in bio-hydrolysis of waste cooking oil	3
Heterologous expression and characterisation of a keratinase produced by Chryseobacterium carnipullorum	3
Refolding, purification, and characterization of constitutive-active human-Smad8 produced as inclusion bodies in ClearColi® BL21 (DE3)	3
Evaluation of the paired-Cas9 nickase and RNA-guided FokI genome editing tools in precise integration of an anti-CD52 bicistronic monoclonal antibody expression construct at Chinese hamster ovary cell	3
Time- and cost-efficient bacterial expression and purification of potato apyrase	3
Efficient periplasmic expression of active lysyl endopeptidase and optimizing the purification methods	3
Identification and characterization of Poly(ADP-ribose) polymerase-1 interacting proteins during development of Dictyostelium discoideum	3

An archaeal nitrile hydratase from the halophilic archaeon A07HB70 exhibits high tolerance to 3-cyanopyridine and nicotinamide	3
Production of functional recombinant prolyl hydroxylase-2 enzyme in insect cells for small molecule inhibitor screening studies	3
Expression, purification and refolding of pro-MMP-2 from inclusion bodies of E. coli	3
Co-expression of recombinant human collagen α1(III) chain with viral prolyl 4-hydroxylase in Pichia pastoris GS115	3
Corrigendum to “MmpL3, the trehalose monomycolate transporter, is stable in solution in several detergents and can be reconstituted into peptidiscs” [Protein Expr. Purif. 191 (March 2022) 106014]	3
Polycistronic baculovirus expression of SUGT1 enables high-yield production of recombinant leucine-rich repeat proteins and protein complexes	3
Cloning and characterization of a novel DNase gene from Trichogramma pretiosum	3
Alternative strategies for the recombinant synthesis, DOPA modification and analysis of mussel foot proteins – A case study for Mefp-3 from Mytilus edulis	3
Developing a secretory AcGFP1-based IRES expression system for efficient production of mammalian recombinant proteins	3
Production of VP3-only virus-like particles of Adeno-associated virus 2 in E. coli cells	3
Recombinant protein production and functional analysis of a M60-like-2 metallopeptidase enzyme from the carcinogenic liver fluke Opisthorchis viverrini	3
Enhancement of recombinant human interleukin-22 production by fusing with human serum albumin and supplementing N-acetylcysteine in Pichia Pastoris	3
Recombinant expression, purification and characterization of human soluble tumor necrosis factor receptor 2	3
Production and compositional analysis of full-length influenza virus hemagglutinin in Nanodiscs: Insights from multi-angle light scattering	3
Antibody-mimetic drug conjugate with efficient internalization activity using anti-HER2 VHH and duocarmycin	3
Improvement of thermostability of cholesterol oxidase from streptomyces Sp. SA-COO by random mutagenesis	3
Highly efficient protein expression of Plasmodium vivax surface antigen, Pvs25, by silkworm and its biochemical analysis	3
Effect of tandem repeats of antimicrobial peptide CC34 on production of target proteins and activity of Pichia pastoris	3
Separation and identification of ACE inhibitory peptides from lizard fish proteins hydrolysates by metal affinity-immobilized magnetic liposome	3
Tandem fusion of albumin-binding domains promoted soluble expression and stability of recombinant trichosanthin in vitro and in vivo	3