OOIR: Observatory of International Research

Papers

(The TQCC of Preparative Biochemistry & Biotechnology is 4. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-08-01 to 2025-08-01.)

Article	Citations
Research on soft sensing method of photosynthetic bacteria fermentation process based on ant colony algorithm and least squares support vector machine	46
Study on covalent coupling process and flow characteristics of antibody on the surface of immunoassay microfluidic chip	38
GFP fusion promotes the soluble and active expression of a pea actin isoform (PEAc1) in Escherichia coli	23
Direct and cost-effective method for histone isolation from cultured mammalian cells	19
Choice of gDNA isolation method has a significant impact on average murine Telomere Length estimates	16
Engineered Saccharomyces cerevisiae harbors xylose isomerase and xylose transporter improves co-fermentation of xylose and glucose for ethanol production	15
Effect of temperature on synthesis of clavulanic acid and impurity substance G during fermentation by Streptomyces clavuligerus	13
New wheat straw fermentation feed: recombinant Schizosaccharomyces pombe efficient degradation of lignocellulose and increase feed protein	13
New application of gelatin and starch as biocarriers for the biodegradation of reactive blue azo dye using immobilized mixed cells	13
Optimization of exopolysaccharide production from the novel Enterococcus species, using statistical design of experiment	13
Production of milk-coagulating protease by fungus Pleurotus djamor through solid state fermentation using wheat bran as the low-cost substrate	12
The effects of different extraction methods on the structure and antioxidant properties of Bletilla striata polysaccharide	12
Biosynthesis and characterization of terpolymer P(3HB- co -3HV- co -4HB) produced by Cupriavidus malaysiensis	12
A novel strategy to construct multi-strain starter cultures: an insight to evolve from natural to directed fermentation	12
Bacteriocin production and inhibition of Bacillus subtilis by Lactobacillus paracasei HD1.7 in an indirect coculture system	12
Transcriptome analysis revealing molecular mechanisms of enhanced pigment yield by succinic acid and fluconazole	12
Antimicrobial peptides (AMPs) from microalgae as an alternative to conventional antibiotics in aquaculture	11
Impacts of conventional and industrial wastewaters-based media on biomass production, nutrient dynamics, and fatty acid profile in a thraustochytrid culture	11
High purity of human secreted phospholipase A2 group IIE in Pichia pastoris using basal salts medium comparison with YPD medium	11
Preparation of esculin acetates through transesterification reaction catalyzed by Novozyme 435 ^® and their Purification followed by NMR characterization	11
In situ recovery of taxadiene using solid adsorption in cultivations with Saccharomyces cerevisiae	11
Xylanase of Bacillus sp. NIORKP76 grown under solid state fermentation: production, purification, characterization and its saccharification potential	11
Detoxification of areca nut acid hydrolysate and production of xylitol by Candida tropicalis (MTCC 6192)	11
Bidirectional fermentation of Monascus and Mulberry leaves enhances GABA and pigment contents: establishment of strategy, studies of bioactivity and mechanistic	11
UV mutagenesis for lipase overproduction from Bacillus cereus ATA179, nutritional optimization, characterization and its usability in the detergent industry	11

Microbial fermentation technology for degradation of saponins from peony seed meal	11
High-level production of poly-γ-glutamic acid by a newly isolated Bacillus sp. YJY-8 and potential use in increasing the production of tomato	11
A secretory system for extracellular production of spider neurotoxin huwentoxin-I in Escherichia coli	11
Optimization and purification of laccase activity from Mammaliicoccus sciuri isolated from the soils of Similipal, Odisha, India: a kinetics study of crystal violet dye	10
Kinetics of dye decolorization using heterogeneous catalytic system with immobilized Achromobacter xylosoxidans DDB6	10
Immobilization of Coprinus comatus with magnetic alginate hydrogel microsphere for improving the antioxidant activity of fermentation products	10
PEGylation versus glycosylation: effect on the thermodynamics and thermostability of crisantaspase	10
Enhancement in T1 lipase purification recovery using the novel construct pGEX4T1/His-T1	9
Studies on a new antimicrobial peptide from Vibrio proteolyticus MT110	9
Insulin-transferrin-selenium promote formation of tissue-engineered vascular grafts in early stage of culture	9
Optimization of the production conditions of tri-GOS and lactosucrose from lactose and sucrose with recombinant β-galactosidase	9
Production of new lipase from Preussia africana and partial characterization	9
Production of reverse transcriptase from Moloney murine Leukemia virus in Escherichia coli expression system	9
Marine sediment derived bacteria Enterobacter asburiae ES1 and Enterobacter sp. Kamsi produce laccase with high dephenolisation potentials	9
A systematic study on the characteristics and applications of laccases produced by fungi: insights on their potential for biotechnologies	9
Enhancement of growth and biomolecules (carbohydrates, proteins, and chlorophylls) of isolated Chlorella thermophila using optimization tools	9
Optimization of alkali, acid and organic solvent pretreatment on rice husk and its techno economic analysis for efficient sugar production	8
Aqueous two-phase extraction and characterization of thermotolerant alkaliphilic Cladophora hutchinsiae xylanase: biochemical properties and potential applications in fr	8
A comprehensive study of glucose and oxygen gradients in a scaled-down model of recombinant HuGM-CSF production in thermoinduced Escherichia coli fed-batch cultures	8
Production of highly soluble native human paraoxonase 2 with potential anti-biofilm property	8
Low cost nutrient-rich oil palm trunk bagasse hydrolysate for bio-succinic acid production by Actinobacillus succinogenes	8
Effects of glycerol and glucose on docosahexaenoic acid synthesis in Aurantiochyrium limacinum SFD-1502 by transcriptome analysis	8
Extracellular collagenase isolated from Streptomyces antibioticus UFPEDA 3421: purification and biochemical characterization	8
In vitro antioxidant activity evaluation of pine nut peptides ( Pinus koraiensis ) fermented by Bacillus subtilis LS-45	8
Optimization of fermentation conditions for production of neutral metalloprotease by Bacillus subtilis SCK6 and its application in goatskin-dehairing	8
Multi-functional xylanase from Aspergillus sydowii : biosynthesis of nanoconjugates, optimization by Taguchi approach and biodeinking potential	8
Enhanced hyaluronic acid production in Streptococcus zooepidemicus by an optimized culture medium containing hyaluronidase inhibitor	8
Enhancing methane yield and shifting microbial communities in anaerobic reactors treating lipid-rich dairy wastewater through exogenous lipase addition	8
Optimization of medium composition of Lactobacillus plantarum Y44 using Plackett – Burman and Box–Behnken designs	8
In-situ transesterification of single-cell oil for biodiesel production: a review	8
Development of a real-time PCR protocol for the detection of chicken DNA in meat products	8
Electrochemical system for detection of fermentation-derived bioethanol via cyclic voltammetry using nickel oxide modified glassy carbon electrode	8
Preparation of magnetic polyacrylamide hydrogel with chitosan for immobilization of glutamate decarboxylase to produce γ-aminobutyric acid	7
Synthesis of new bio-activated carbon filled epoxy composites	7
Enhanced phytoremediation and biosorption of cationic methylene blue dye by Acidomyces acidophilus ATCC 26774	7
Development of an efficient extraction and enrichment method for total flavonoids compounds from Erigeron breviscapus using ultrasound-assisted extraction and macroporous resin adsorption	7
Two modified density gradient centrifugation methods facilitate the isolation of mouse Leydig cells	7
Optimization of lyoprotectant for recombinant human acidic fibroblast growth factor by response surface methodology	7
Production of tannase from a newly isolated yeast, Geotrichum cucujoidarum using agro-residues	7
Isolation and characterization of yogurt starter cultures from traditional yogurts and growth kinetics of selected cultures under lab-scale fermentation	7
Purification and characterization of avocado (Persea americana) polyphenol oxidase by affinity chromatography	7
Measurement of yield and quality of DNA in human buffy coat is extraction method dependent	7
Experimental validation off-line a nonlinear controller for removal of chromium using non-living cells of Yarrowia lipolytica	7
Cellulase production by Aspergillus fumigatus A4112 and the potential use of the enzyme in cooperation with surfactant to enhance floating oil recovery and methane produ	7
Efficient whole-cell biosynthesis of S-adenosyl-L-methionine by the engineered Escherichia coli with high ATP regenerating system	7
One-pot green and sustainable process for the biotransformation of sophoricoside to genistein from Fructus Sophorae with magnetic cellulose spheres immobilized	7
Enhanced production of yellow fever virus through tailored culture media optimization	7
Agro-industrial wastes revalorization as feedstock: production of lignin-modifying enzymes extracts by solid-state fermentation using white rot fungi	6
Microbased biorefinery for gold nanoparticle production: recent advancements, applications and future aspects	6
Enhancement of bacterial cellulose production by ethanol and lactic acid by using Gluconacetobacter kombuchae	6

Expression of Vitreoscilla hemoglobin gene in different organisms: a new approach to increase the efficiency of various biological processes	6
Immobilization, optimization, characterization and kinetic properties of polyphenol oxidase to multi-walled carbon nanotube	6
Hyaluronic acid production and purification techniques: a review	6
Adsorption and immobilization of β-glucosidase from Thermoascus aurantiacus on macroporous cryogel by hydrophobic interaction	6
Purification of chitosanases produced by Bacillus toyonensis CCT 7899 and functional oligosaccharides production	6
Deep eutectic solvent assisted recovery of high molecular weight levan from an isolated Neobacillus pocheonensis BPSCM4	6
The multiple-response modeling of heat-assisted, microwave-assisted and subcritical water extraction on selected phenolics from traditional plant speciesTeucrium montanum	6
First report of collagenase production by Trichosporon sp. strain isolated from pollen of Amazonian bee (Melipona seminigra seminigra)	6
Biosynthesis of zinc oxide nanoparticles using Tamarix chinensis extract: evaluation of antioxidant, antimicrobial, and anti-MCF-7 cancer activities	6
Promising approaches in the extraction, characterization, and biotechnological applications of ursolic acid: a review	6
Extraction of total antioxidants from Azadirachta indica (neem) using three phase partitioning and its process intensification using ultrasound	6
Chitinase induction in Trichoderma harzianum : a solid-state fermentation approach using shrimp waste and wheat bran/commercial chitin for chitooligosaccharides synthesi	6
Characterization of a novel marine microbial uricase from Priestia flexa and evaluation of the effects of CMCS conjugation on its enzymatic properties	6
Comparative bioreactor studies of different process enhancement methods in B. licheniformis for enzyme co-production	6
Enhancing the production of L-proline in recombinant Escherichia coli BL21 by metabolic engineering	5
Synergistic utilization of glucose and xylose for the myo-inositol biosynthesis in recombinant Escherichia coli BL21	5
Exploring the antioxidant potential of fermented turmeric pulp: effect of extraction methods and microencapsulation	5
Harnessing algal biomass for sustainable energy: cultivation, strain improvement, and biofuel production	5
Efficient production of ε-poly-l-lysine using cassava starch and fish meal by Streptomyces albulus FQC-24	5
Statistical optimization of process variables for agarase production using Microbacterium sp. SS5 strain from non-marine sources	5
A new hydrophobin candidate from Cladosporium macrocarpum with super-hydrophobic surface	5
Evaluation of the effect of different organic solvents in the efficiency of the recovery of pullulan synthesized by Aureobasidium pullulans	5
Optimization of ultrasound- and enzymatic-assisted extractions of polyphenols from dried red onion peels and evaluation of their antioxidant activities	5
Analytical methods in fatty acid analysis for microbial applications: the recent trends	5
Evaluation of different nutrient limitation strategies for the efficient production of poly(hydroxybutyrate‐co‐hydroxyvalerate) from waste frying oil and propionic acid in high cell density fermentati	5
Optimization of fermentation medium and conditions for enhancing valinomycin production by Streptomyces sp. ZJUT-IFE-354	5
Immobilization of Aspergillus terreus URM4658 inulinase in calcium alginate beads, evaluation of their biochemical characteristics and kinetic/thermodynamic parameters,	5
Acetic acid acting as a signaling molecule in the quorum sensing system increases 2,3-butanediol production in Saccharomyces cerevisiae	5
Optimization of asymmetric bioreduction conditions of 1-(thiophen-2-yl)ethanone by Weissella cibaria N9 using a desirability function-embedded face-centered optimization	5
Production of a genetically encoded biosensor for evaluating enterovirus 71 3C protease inhibitors	5
Antibacterial and antibiofilm activity of extracts from sponge-associated bacterial endophytes	5
Removal of orange G dye by Aspergillus niger and its effect on organic acid production	5
Purification of damson plum polyphenol oxidase by affinity chromatography and investigation of metal effects on enzyme activity	5
Optimizing the extraction of active components from Salvia miltiorrhiza by combination of machine learning models and intelligent optimization algorithms and its correla	5
Cost-effective, high-yield production of Pyrobaculum calidifontis DNA polymerase for PCR application	5
Bioconversion of mustard oil cake for production of lipase, optimization and direct immobilization from solid-state fermentation extract	5
Zinc oxide nanoparticle mediated modulation of antimicrobial and physico-chemical properties of essential oil containing PVA nanocomposites	4
Expression and characterization of a thermostable monoacylglycerol lipase from thermophilic Geobacillus kaustophilus	4
Disposable glutamate biosensor based on platinum nanoparticles, carbon quantum dots and poly-L-aspartic acid	4
Production of bioactive recombinant monoclonal antibody fragment in periplasm of Escherichia coli expression system	4
Techno-economic analysis of production and purification of lipase from Bacillus subtilis (NCIM 2193)	4
Purification and characterization of Stenotrophomonas maltophilia chitinase with antifungal and insecticidal properties	4
Kinetic and thermodynamic investigation of Rhodanese synthesized by enhanced Klebsiella oxytoca JCM 1665 strain: a comparative between the free and immobilized enzyme en	4
Preparation of 5-hydroxymethylfurfural from cellulose catalyzed by silicon-based composite solid acids	4
Sequential mild acid and alkali pretreatment of rice straw to improve enzymatic saccharification for bioethanol production	4
A review on pretreatment methods for lipid extraction from microalgae biomass	4
Microbial surfactants: characteristics, production and broader application prospects in environment and industry	4
Production and characterization of yeast extracts produced by Saccharomyces cerevisiae, Saccharomyces boulardii and Kluyveromyces marxianus	4
Molecular dynamics simulation and validation of spiramycin extraction using the thermosensitive polymer NPE-108/water aqueous two-phase system	4
Application of a 3D printed miniaturized hydrocyclone in biopharmaceutical industry-numerical and experimental studies of yeast separation from fermentation culture media	4
Aligned with sustainable development goals: microwave extraction of astaxanthin from wet algae and selective cytotoxic effect of the extract on lung cancer cells	4
Regulatory considerations in biosimilars: Middle East and Africa regions	4
Optimization of the extraction process of total steroids from Phillinus gilvus (Schwein.) Pat. by artificial neural network (ANN)-response surface methodology and identi	4
Enhanced identification of glycoproteins and allergens in Hevea brasiliensis latex using innovative enrichment techniques	4
Unraveling the biological potential of chicken viscera proteins: a study based on their enzymatic hydrolysis to obtain hydrolysates with antioxidant properties	4
Engineering Escherichia coli to metabolize sorbitol as the sole carbon source for synthesis of recombinant L-Asparaginase-II	4
Synergistic hydrolysis of lignocellulosic biomass using co-immobilization of tri-enzymes on chitosan-magnetite nanoparticle beads	4
Ethanol and 2-phenylethanol production by bee-isolated Meyerozyma caribbica strains	4
Optimization of enzymatic production of anti-skin aging biopeptides from white sorghum [ Sorghum bicolor (L) Moench] grain	4
Ultrasonic/microwave-assisted extraction and properties of polysaccharides from Elaeagnus angustifolia	4
The evaluation of the performance of rice husk and rice straw as potential matrix to obtain the best lipase immobilized system: creating wealth from wastes	4
Role of key enzymes in the production of docosahexaenoic acid (DHA) by Thraustochytrium sp. T01	4
Engineered production of pyridoxal 5′-phosphate in Escherichia coli BL21	4
In vitro fermentation assay on the bifidogenic effect of steviol glycosides of Stevia rebaudiana plant for the development of dietetic novel products	4
Fractional mixture design: a novel design and methodology for analyzing and optimizing mixtures of several ingredients	4
Changes in the structure and composition of pineapple leaf fiber after alkali and ionic surfactant pretreatments and their impact on enzymatic hydrolysis	4
Optimization of culture medium and scale-up production of astaxanthin using corn steep liquor as substrate by response surface methodology	4
Enhancement of anti-TNFα monoclonal antibody production in CHO cells through the use of UCOE and DHFR elements in vector construction and the optimization of cell culture media	4
Efficient RNA extraction method for acquiring high-quality RNA from various tissues of the fiber crop abaca, Musa textilis Née	4
Solid-state bioprocessing of sugarcane bagasse with Auricularia fuscosuccinea for phenolic compounds extraction	4
Engineered production of 5-aminolevulinic acid in recombinant Escherichia coli BL21	4
The effects of different protease treatments on the techno-functional, structural, and bioactive properties of bovine casein	4
Recombinant expression and tryptophan-assisted analysis of human sweet taste receptor T1R3’s extracellular domain in sweetener interaction studies	4
Application of a constrained mixture design for lipase production by Penicillium roqueforti ATCC 10110 under solid-state fermentation and using agro-industrial wastes as substrate	4
Optimizing the extraction of high-potency antioxidants from Solanum lycopersicum L. fruit: a comparative study of microwave-assisted, ultrasound-assisted and accelerated	4
Extraction and purification of Aspergillus tamarii β-fructofuranosidase with transfructosylating activity using aqueous biphasic systems (PEG/phosphate) and magnetic field	4