Nature Biotechnology

Article	Citations
TRGT-ing the dark genome to accurately characterize tandem repeats at scale	2045
The champions of drug development	1833
People	1265
Cancer Moonshot aims at liquid biopsies	1219
Author Correction: LAMP-Seq enables sensitive, multiplexed COVID-19 diagnostics using molecular barcoding	789
Integrating medicine, engineering and business to educate early-stage researchers in cardiovascular device development	666
AI’s plastic recycling	642
Fluorescence microscopy resolution increases to near-atomic level	623
Single-cell RNA-seq keeps cells alive	619
Fungal architecture is a thing, and it’s eye-catching	553
Dreaming ideal protein structures	538
Synthetic biologics provide a path to target bHLH transcription factors	502
Author Correction: An injectable bone marrow–like scaffold enhances T cell immunity after hematopoietic stem cell transplantation	501
An optimized toolkit for prime editing	478
Overcoming unpredictability in biotech patents by tailored claiming aligned with genuine enablement	463
Drug pipeline 1Q22 — a raft of new modalities … and clinical blowups	426
Framework for predicting alloreactivity in hematopoietic cell transplants	388
Author Correction: Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion	363
Behind the graduate mental health crisis in science	361
Denmark invests in mucosal vaccines	351
Wireless microelectronic fibers to discover gut and brain pathways	348
People	342
Bite-sized solutions	333
Negative innovation: when patents are bad for patients	328
Reflective multi-immersion microscope objectives inspired by the Schmidt telescope	321

Enhancing prime editing in hematopoietic stem and progenitor cells by modulating nucleotide metabolism	313
A competitive precision CRISPR method to identify the fitness effects of transcription factor binding sites	305
Safer non-viral DNA delivery using lipid nanoparticles loaded with endogenous anti-inflammatory lipids	301
Predicting transcriptional outcomes of novel multigene perturbations with GEARS	293
Cellular shuttles put mislocalized proteins in their right place	283
Dual SORT LNPs for multi-organ base editing	279
Engineered serum markers for non-invasive monitoring of gene expression in the brain	279
Diffusion model expands RoseTTAFold’s power	274
Biopharma dives into tumor-seeking radioactive drugs	258
Chem-map profiles drug binding to chromatin in cells	256
Automated reconstruction of whole-embryo cell lineages by learning from sparse annotations	251
Democratizing knowledge representation with BioCypher	250
Engineering innate immune cells for cancer immunotherapy	246
Droplet-based transcriptome profiling of individual synapses	244
In vivo development of immune tissue in human intestinal organoids transplanted into humanized mice	243
A small and highly sensitive red/far-red optogenetic switch for applications in mammals	239
Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome	237
The lysosomal degraders	228
The world’s first CRISPR therapy is approved: who will receive it?	213
Generative models for protein structures and sequences	211
Multi-omics single-cell data integration and regulatory inference with graph-linked embedding	205
Artificial intelligence-guided design of lipid nanoparticles for pulmonary gene therapy	202
High-efficiency transgene integration by homology-directed repair in human primary cells using DNA-PKcs inhibition	193
An oncolytic virus–T cell chimera for cancer immunotherapy	191
3Q22 — more malaise in the markets	187
Variant to function mapping at single-cell resolution through network propagation	187
Biotech news from around the world	186
The need for need-finding in medical education	183
Light-sheet microscopy at high resolution	183
Author Correction: The cost of drug patent expiration date errors	178
Live cell imaging of signaling networks using a conventional microscope	174
Single-cell nanobody-based profiles of multiple epigenetic modalities and chromatin velocity	171
Delix Therapeutics : psychedelics without the trip	169
AI predicts cancer relapse from slides	168
Biotech news from around the world	166
Towards the non-invasive imaging of brain networks and functions at high resolution	166
Cancer diagnostics	165
People	165
Next-generation psychedelics: should new agents skip the trip?	164
Who will oversee the ethical limits of human embryo research?	162
Nanotechnology offers alternative ways to fight COVID-19 pandemic with antivirals	161
Assessing workforce needs for the emerging CAR-T cell therapy industry	161
Precise DNA cleavage using CRISPR-SpRYgests	160
Gains in early-onset dementia with progranulin open new paths for drug discovery	160
Hallucinating functional protein sequences	160
Decoding gene regulation with CRISPR perturbations	159
FDA approves first MASH drug	158
Adrestia Therapeutics — Gene networks to the rescue	156
Delivering 3 billion doses of Comirnaty in 2021	153
Prediction of peptide mass spectral libraries with machine learning	152

Slaughter-free meat hits the grocery shelf	152
Orna Therapeutics: circular logic	150
Retraction Note: Rescue of the spinal muscular atrophy phenotype in a mouse model by early postnatal delivery of SMN	149
HSV-1’s contribution as a vector for gene therapy	148
Protection of cell therapeutics from antibody-mediated killing by CD64 overexpression	146
Computationally designed enzymes show potent catalytic activity	145
Grand challenges in bioinformatics education and training	143
Engineered pegRNAs improve prime editing efficiency	142
A language model beats alphafold2 on orphans	142
Mapping single-cell data to reference atlases by transfer learning	141
Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes	140
Engineered phage with antibacterial CRISPR–Cas selectively reduce E. coli burden in mice	139
Improving non-viral knock-in with modified single-stranded DNAs and small molecules	139
Detection of mosaic and population-level structural variants with Sniffles2	139
Top 20 translational researchers of 2020	138
2022 – toughing out the trough	138
Biotech news from around the world	138
Drug pipeline 4Q22 — sticking around	138
Chemigenetic kinase biosensors illuminate cell signaling networks	136
A strategy to load, rethread and read protein sequences through a nanopore	135
Enzymatic synthesis of RNA oligonucleotides	135
Tension-tuned receptors for synthetic mechanotransduction and intercellular force detection	134
CoSpar identifies early cell fate biases from single-cell transcriptomic and lineage information	134
Chromatin Velocity reveals epigenetic dynamics by single-cell profiling of heterochromatin and euchromatin	132
Spatial host–microbiome sequencing reveals niches in the mouse gut	130
Lab-made antibody stops malaria	129
100,000 whole-genome sequences’ diagnostic bonus	129
Forum: Boyden and Danzl	129
Biotech news from around the world	129
Moderna feud with NIH over COVID vaccine	128
COVID vaccine IP waiver agreed	128
Synthetic virology: the experts speak	128
Are ecological processes that select beneficial traits in agricultural microbes nature’s intellectual property rights?	125
The devolution of biosimilars regulations	123
Consternation follows Marks’ FDA departure	123
Growth of biotech clusters over several decades through pioneering, variety and entrepreneurial science	123
Baby’s first genome	122
Large-scale discovery of chromatin dysregulation induced by oncofusions and other protein-coding variants	121
Rapid, scalable assessment of SARS-CoV-2 cellular immunity by whole-blood PCR	120
Microbiome-friendly phages join the campaign for better antimicrobials	120
Platform solutions for commercial challenges to expanding patient access and making gene editing sustainable	119
Control of mammalian gene expression by modulation of polyA signal cleavage at 5′ UTR	118
A circularly permuted CasRx platform for efficient, site-specific RNA editing	118
Data sharing in the age of deep learning	115
Precision RNA base editing with engineered and endogenous effectors	113
Prediction of prime editing insertion efficiencies using sequence features and DNA repair determinants	113
Epicardioid single-cell genomics uncovers principles of human epicardium biology in heart development and disease	112
A fully integrated wearable ultrasound system to monitor deep tissues in moving subjects	111
Genome engineering with Cas9 and AAV repair templates generates frequent concatemeric insertions of viral vectors	110
Contamination source modeling with SCRuB improves cancer phenotype prediction from microbiome data	109
Modular cytokine receptor-targeting chimeras for targeted degradation of cell surface and extracellular proteins	108
How will generative AI disrupt data science in drug discovery?	107
Author Correction: Bioinstructive implantable scaffolds for rapid in vivo manufacture and release of CAR-T cells	106
Antivirals	106
Base editors	106
Publisher Correction: Treg engineers take aim at autoimmunity	104
Forum: Drug delivery roundtable with Langer and Whitehead	104
Relieving patent-eligibility barriers in biotech with a preparation or treatment method	104
Ionis wins FDA nod for triglyceride-lowering rare disease drug	104
An organoid model with all three pancreatic lineages resembles fetal pancreas	103
How will AI affect patent disclosures?	103
Five questions with César de la Fuente	102
2024: research in review	102
People	101
Stem cells	101
Publisher Correction: Send in the senolytics	100
Sensitive detection of gene transfer in a microbial community	98
Author Correction: Engineering circular RNA for enhanced protein production	97
A genome-wide view of disordered proteins	96
Reply to: Methodological concerns and lack of evidence for single-synapse RNA-seq	96
Intestinal mucosal barrier repair and immune regulation with an AI-developed gut-restricted PHD inhibitor	96
Septerna: making another run on GPCRs	94
Spotlight on cancer immunotherapies	93
TCR cell therapies vanquish solid tumors — finally	93
Mapping medically relevant RNA isoform diversity in the aged human frontal cortex with deep long-read RNA-seq	92
CRISPR beef cattle get FDA green light	92
Author Correction: Precise, predictable multi-nucleotide deletions in rice and wheat using APOBEC–Cas9	91
Senolytics under scrutiny in the quest to slow aging	91
High-precision cytosine base editors by evolving nucleic-acid-recognition hotspots in deaminase	91
Thinking about a move from academia to industry? Look before you leap	91

Next-generation patient models for colorectal cancer research	90
Tracking gene order provides a new perspective on intraspecific evolution in microbiotas	90
Genotoxins from gut bacteria	89
Generation of accurate, expandable phylogenomic trees with uDance	89
Spatial genomics of AAV vectors reveals mechanism of transcriptional crosstalk that enables targeted delivery of large genetic cargo	87
Post-translational modifications reshape the antigenic landscape of the MHC I immunopeptidome in tumors	87
Discovery of drug–omics associations in type 2 diabetes with generative deep-learning models	86
AI-enhanced protein design makes proteins that have never existed	85
The strength and importance of government-funded patents for approved drugs	85
High-throughput discovery of MHC class I- and II-restricted T cell epitopes using synthetic cellular circuits	84
Precise integration of large DNA sequences in plant genomes using PrimeRoot editors	84
Facile repurposing of peptide–MHC-restricted antibodies for cancer immunotherapy	84
Human respiratory airway progenitors derived from pluripotent cells generate alveolar epithelial cells and model pulmonary fibrosis	84
Accelerated identification of disease-causing variants with ultra-rapid nanopore genome sequencing	83
Multifunctional microelectronic fibers enable wireless modulation of gut and brain neural circuits	83
A community effort to optimize sequence-based deep learning models of gene regulation	83
Integration of spatial and single-cell transcriptomic data elucidates mouse organogenesis	82
Hypoimmune induced pluripotent stem cells survive long term in fully immunocompetent, allogeneic rhesus macaques	82
The GA4GH Phenopacket schema defines a computable representation of clinical data	82
Efficient prime editing in mouse brain, liver and heart with dual AAVs	82
Tuning plant phenotypes by precise, graded downregulation of gene expression	81
Conformation-locking antibodies for the discovery and characterization of KRAS inhibitors	79
3Q23 — VCs feed ‘older children’ as IPO markets stagnate	78
Combining reference genomes into a pangenome graph improves accuracy and reduces bias	78
Clarifying US regulations on xenotransplantation	78
2H23 biotech job picture	78
Fashion’s microbial dyeing machines	77
People	77
Biotech news from around the world	77
Starving tumors with fat	77
Base editor restores CD3δ-dependent SCID mutation	77
Measuring the impact of chromatin context on transcription factor binding affinities	77
Microbiomics	76
Redefining antibody patent protection using paratope mapping and CDR-scanning	76
The instant spray-on dress	76
Bridge RNAs direct programmable DNA rearrangements	76
Microfluidics	76
Biotech patenting 2021	76
Publisher Correction: Top 20 translational researchers of 2020	75
Startup grows egg proteins in potato fields	75
Wearable ultrasound for continuous deep-tissue monitoring	75
The challenges and promise of sweat sensing	74
Generation of a live attenuated influenza A vaccine by proteolysis targeting	74
Lipid nanoparticle-mediated mRNA delivery to CD34+ cells in rhesus monkeys	74
Curated variation benchmarks for challenging medically relevant autosomal genes	74
Fighting fibrosis	73
Discovering the crucial function of long noncoding RNAs	72
How to grow cement	71
De novo detection of somatic mutations in high-throughput single-cell profiling data sets	71
Chikungunya vaccine approved	71
Eli Lilly in $650M pact to boost muscle	71
Bio-leather gears up to wow fashion industry	70
A shifting market environment and strategies fueling innovation in prostate cancer treatment	70
Prime editing	70
Resurrecting a miniature Cas9 ancestor for genome and epigenome editing	70
Make science disruptive again	70
Combination therapy patents: a new front in evergreening	69
Uniform volumetric single-cell processing for organ-scale molecular phenotyping	69
High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails	69
Single-molecule live-cell RNA imaging with CRISPR–Csm	68
Linking CRISPR–Cas9 double-strand break profiles to gene editing precision with BreakTag	68
Deep tissue multi-photon imaging using adaptive optics with direct focus sensing and shaping	68
Using artificial intelligence to develop gene therapy for the lungs	67
Capturing the diversity of protein modifications on presented tumor antigens	67
The DxConnect Virtual Biobank connects diagnostic researchers to clinical samples	67
Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity	67
Challenges for developing broad-based mucosal vaccines for respiratory viruses	67
The reverse transcriptase domain of prime editors contributes to DNA repair in mammalian cells	67
Scalable single-cell RNA sequencing from full transcripts with Smart-seq3xpress	66
Fresh from the biotech pipeline: FDA approvals settle in 2024, but what next?	66
A highly photostable and bright green fluorescent protein	66
Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases	66
A germ-free humanized mouse model shows the contribution of resident microbiota to human-specific pathogen infection	65
Ultra-fast label-free quantification and comprehensive proteome coverage with narrow-window data-independent acquisition	64
Biotech news from around the world	64
RNA interference in the era of nucleic acid therapeutics	64
Visualizing protein nanostructures in intact brain	64
People	63
Public biotech in 2021 — the numbers	62
Biotech news from around the world	62
First in vivo base editing lowers cholesterol	61
Second-quarter biotech job picture	61
Forum: Locke and Sykes	61
Biomedical imaging	61
A retrotransposon for site-specific gene transfer	60
Insulin — the new battleground for drug pricing	60
The long game of tenure	59
Wasp gut microbes yield beer with extra tang	58
Sizing up beta cells made from stem cells	58
Author Correction: m6A RNA modifications are measured at single-base resolution across the mammalian transcriptome	58
Large-scale evaluation of the ability of RNA-binding proteins to activate exon inclusion	57
Innovators want pills to treat sickle cell disease. Can they match gene therapy?	57
A way forward for diagnosis of patients with extremely rare genetic mutations	57
Reprogramming endogenous regulatory DNA to fine-tune gene expression	56
The emerging landscape of engineered bacteria cancer therapies	56