Nature Biotechnology

Article	Citations
Single-cell RNA-seq keeps cells alive	3252
Fungal architecture is a thing, and it’s eye-catching	2236
Fluorescence microscopy resolution increases to near-atomic level	1740
Synthetic biologics provide a path to target bHLH transcription factors	949
AI’s plastic recycling	948
Author Correction: LAMP-Seq enables sensitive, multiplexed COVID-19 diagnostics using molecular barcoding	816
People	788
TRGT-ing the dark genome to accurately characterize tandem repeats at scale	676
Denmark invests in mucosal vaccines	645
Wireless microelectronic fibers to discover gut and brain pathways	627
Overcoming unpredictability in biotech patents by tailored claiming aligned with genuine enablement	600
Drug pipeline 1Q22 — a raft of new modalities … and clinical blowups	555
Framework for predicting alloreactivity in hematopoietic cell transplants	444
Bite-sized solutions	434
An optimized toolkit for prime editing	416
Design of optimized epigenetic regulators for durable gene silencing with application to PCSK9 in nonhuman primates	413
People	408
Orchestration of secondary metabolite production in Streptomyces species	399
The lysosomal degraders	395
Engineered serum markers for non-invasive monitoring of gene expression in the brain	394
Cellular shuttles put mislocalized proteins in their right place	384
Diffusion model expands RoseTTAFold’s power	366
Integrating medicine, engineering and business to educate early-stage researchers in cardiovascular device development	351
Dreaming ideal protein structures	343
Cancer Moonshot aims at liquid biopsies	324

A competitive precision CRISPR method to identify the fitness effects of transcription factor binding sites	319
Author Correction: Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew	317
Enhancing prime editing in hematopoietic stem and progenitor cells by modulating nucleotide metabolism	309
Democratizing knowledge representation with BioCypher	301
Droplet-based transcriptome profiling of individual synapses	294
Author Correction: Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion	283
Automated reconstruction of whole-embryo cell lineages by learning from sparse annotations	274
An oncolytic virus–T cell chimera for cancer immunotherapy	274
Generative models for protein structures and sequences	263
Chem-map profiles drug binding to chromatin in cells	251
Biopharma dives into tumor-seeking radioactive drugs	243
Reflective multi-immersion microscope objectives inspired by the Schmidt telescope	240
Behind the graduate mental health crisis in science	230
High-efficiency transgene integration by homology-directed repair in human primary cells using DNA-PKcs inhibition	229
Programmable promoter editing for precise control of transgene expression	218
Engineering innate immune cells for cancer immunotherapy	217
In vivo development of immune tissue in human intestinal organoids transplanted into humanized mice	214
The world’s first CRISPR therapy is approved: who will receive it?	212
Safer non-viral DNA delivery using lipid nanoparticles loaded with endogenous anti-inflammatory lipids	210
Variant to function mapping at single-cell resolution through network propagation	200
Artificial intelligence-guided design of lipid nanoparticles for pulmonary gene therapy	200
Predicting transcriptional outcomes of novel multigene perturbations with GEARS	199
Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome	198
Multi-omics single-cell data integration and regulatory inference with graph-linked embedding	198
Single-cell nanobody-based profiles of multiple epigenetic modalities and chromatin velocity	196
Dual SORT LNPs for multi-organ base editing	196
AI predicts cancer relapse from slides	193
Biotech news from around the world	192
3Q22 — more malaise in the markets	191
The need for need-finding in medical education	190
Author Correction: The cost of drug patent expiration date errors	189
People	188
Biotech news from around the world	188
Gains in early-onset dementia with progranulin open new paths for drug discovery	185
HSV-1’s contribution as a vector for gene therapy	182
Slaughter-free meat hits the grocery shelf	181
Retraction Note: Rescue of the spinal muscular atrophy phenotype in a mouse model by early postnatal delivery of SMN	181
Who will oversee the ethical limits of human embryo research?	181
Delix Therapeutics : psychedelics without the trip	178
Towards the non-invasive imaging of brain networks and functions at high resolution	177
Computationally designed enzymes show potent catalytic activity	174
Adrestia Therapeutics — Gene networks to the rescue	172
Connecting research and clinical practice: training the next generation of translational scientists	170
Orna Therapeutics: circular logic	170
Artificial miRNA slows Huntington’s	169
Assessing workforce needs for the emerging CAR-T cell therapy industry	169
Hallucinating functional protein sequences	169
Decoding gene regulation with CRISPR perturbations	167
David Baltimore (1938–2025)	167
Building synthetic chromosomes one yeast at a time: insights from Sc2.0	167

Mapping the patent landscape of cancer diagnostics	165
Prediction of peptide mass spectral libraries with machine learning	163
Grand challenges in bioinformatics education and training	162
Next-generation psychedelics: should new agents skip the trip?	162
Live cell imaging of signaling networks using a conventional microscope	161
Protection of cell therapeutics from antibody-mediated killing by CD64 overexpression	161
Precise DNA cleavage using CRISPR-SpRYgests	159
A language model beats alphafold2 on orphans	159
Delivering 3 billion doses of Comirnaty in 2021	157
Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes	157
Engineered phage with antibacterial CRISPR–Cas selectively reduce E. coli burden in mice	156
FDA approves first MASH drug	154
Mesenchymal thymic niche cells enable regeneration of the adult thymus and T cell immunity	151
Detection of mosaic and population-level structural variants with Sniffles2	150
A strategy to load, rethread and read protein sequences through a nanopore	150
Drug pipeline 4Q22 — sticking around	145
2022 – toughing out the trough	145
Chemigenetic kinase biosensors illuminate cell signaling networks	144
A circularly permuted CasRx platform for efficient, site-specific RNA editing	143
Biotech news from around the world	142
Forum: Boyden and Danzl	141
Lab-made antibody stops malaria	140
Are ecological processes that select beneficial traits in agricultural microbes nature’s intellectual property rights?	138
Genome-edited farm animals from haploid stem cells	138
Microbiome-friendly phages join the campaign for better antimicrobials	131
Large-scale discovery of chromatin dysregulation induced by oncofusions and other protein-coding variants	130
Consternation follows Marks’ FDA departure	128
COVID vaccine IP waiver agreed	128
Enzymatic synthesis of RNA oligonucleotides	127
Engineering pattern recognition receptors facilitates plant resistance breeding	127
Baby’s first genome	126
RNA stability enhancers for durable base-modified mRNA therapeutics	124
Precision RNA base editing with engineered and endogenous effectors	121
Tension-tuned receptors for synthetic mechanotransduction and intercellular force detection	120
Prediction of prime editing insertion efficiencies using sequence features and DNA repair determinants	120
Data sharing in the age of deep learning	119
Growth of biotech clusters over several decades through pioneering, variety and entrepreneurial science	119
How will generative AI disrupt data science in drug discovery?	118
Control of mammalian gene expression by modulation of polyA signal cleavage at 5′ UTR	118
Rapid, scalable assessment of SARS-CoV-2 cellular immunity by whole-blood PCR	118
Chronic nasal congestion gets new treatment	116
Platform solutions for commercial challenges to expanding patient access and making gene editing sustainable	116
The devolution of biosimilars regulations	114
Improving non-viral knock-in with modified single-stranded DNAs and small molecules	113
Editor’s pick: Red Queen Therapeutics	111
Cell type inference in cell-free nucleic acid liquid biopsy	110
A fully integrated wearable ultrasound system to monitor deep tissues in moving subjects	110
Modular cytokine receptor-targeting chimeras for targeted degradation of cell surface and extracellular proteins	109
Spatial host–microbiome sequencing reveals niches in the mouse gut	108
Epicardioid single-cell genomics uncovers principles of human epicardium biology in heart development and disease	108
Contamination source modeling with SCRuB improves cancer phenotype prediction from microbiome data	107
CoSpar identifies early cell fate biases from single-cell transcriptomic and lineage information	106
Antivirals	105
Genome engineering with Cas9 and AAV repair templates generates frequent concatemeric insertions of viral vectors	105
Base editors	104
Sensitive detection of gene transfer in a microbial community	103
Author Correction: Bioinstructive implantable scaffolds for rapid in vivo manufacture and release of CAR-T cells	103
Publisher Correction: Treg engineers take aim at autoimmunity	103
Stem cells	102
2024: research in review	102
People	102
Ionis wins FDA nod for triglyceride-lowering rare disease drug	101
An organoid model with all three pancreatic lineages resembles fetal pancreas	100
Relieving patent-eligibility barriers in biotech with a preparation or treatment method	100
Reply to: Methodological concerns and lack of evidence for single-synapse RNA-seq	99
A genome-wide view of disordered proteins	98
CRISPR beef cattle get FDA green light	98
Thinking about a move from academia to industry? Look before you leap	97
Senolytics under scrutiny in the quest to slow aging	97
Author Correction: Precise, predictable multi-nucleotide deletions in rice and wheat using APOBEC–Cas9	96
Tracking gene order provides a new perspective on intraspecific evolution in microbiotas	95
Next-generation patient models for colorectal cancer research	95
Five questions with César de la Fuente	94
Generation of accurate, expandable phylogenomic trees with uDance	94
Children with rare genetic diseases get CRISPR Cures center	93
Septerna: making another run on GPCRs	93
Conformation-locking antibodies for the discovery and characterization of KRAS inhibitors	92
Accurate somatic small variant discovery for multiple sequencing technologies with DeepSomatic	92
Growth-coupled microbial biosynthesis of the animal pigment xanthommatin	92
Author Correction: Engineering circular RNA for enhanced protein production	92

TCR cell therapies vanquish solid tumors — finally	92
The strength and importance of government-funded patents for approved drugs	91
How will AI affect patent disclosures?	90
Spatial genomics of AAV vectors reveals mechanism of transcriptional crosstalk that enables targeted delivery of large genetic cargo	89
AI-enhanced protein design makes proteins that have never existed	89
Accelerated identification of disease-causing variants with ultra-rapid nanopore genome sequencing	89
Cross-species snakebite antivenom	87
Hypoimmune induced pluripotent stem cells survive long term in fully immunocompetent, allogeneic rhesus macaques	87
Facile repurposing of peptide–MHC-restricted antibodies for cancer immunotherapy	86
The GA4GH Phenopacket schema defines a computable representation of clinical data	86
Spotlight on cancer immunotherapies	86
Genotoxins from gut bacteria	86
Efficient prime editing in mouse brain, liver and heart with dual AAVs	85
High-precision cytosine base editors by evolving nucleic-acid-recognition hotspots in deaminase	84
Systema: a framework for evaluating genetic perturbation response prediction beyond systematic variation	84
High-throughput discovery of MHC class I- and II-restricted T cell epitopes using synthetic cellular circuits	84
A community effort to optimize sequence-based deep learning models of gene regulation	84
Intestinal mucosal barrier repair and immune regulation with an AI-developed gut-restricted PHD inhibitor	84
Precise integration of large DNA sequences in plant genomes using PrimeRoot editors	83
Mapping medically relevant RNA isoform diversity in the aged human frontal cortex with deep long-read RNA-seq	83
Discovery of drug–omics associations in type 2 diabetes with generative deep-learning models	83
Human respiratory airway progenitors derived from pluripotent cells generate alveolar epithelial cells and model pulmonary fibrosis	83
Tuning plant phenotypes by precise, graded downregulation of gene expression	82
Multifunctional microelectronic fibers enable wireless modulation of gut and brain neural circuits	82
Post-translational modifications reshape the antigenic landscape of the MHC I immunopeptidome in tumors	81
Fashion’s microbial dyeing machines	81
3Q23 — VCs feed ‘older children’ as IPO markets stagnate	81
2H23 biotech job picture	80
People	80
Measuring the impact of chromatin context on transcription factor binding affinities	80
Microbiomics	79
Microfluidics	79
Discovering the crucial function of long noncoding RNAs	79
Base editor restores CD3δ-dependent SCID mutation	79
Bridge RNAs direct programmable DNA rearrangements	78
The instant spray-on dress	78
Biotech patenting 2021	77
Startup grows egg proteins in potato fields	77
Chikungunya vaccine approved	76
Combining reference genomes into a pangenome graph improves accuracy and reduces bias	76
Big pharma seduced by transcription factors again. What has changed?	76
Embryonic stem cells across bird species	76
Fighting fibrosis	75
Using artificial intelligence to develop gene therapy for the lungs	75
Redefining antibody patent protection using paratope mapping and CDR-scanning	74
A shifting market environment and strategies fueling innovation in prostate cancer treatment	73
Resurrecting a miniature Cas9 ancestor for genome and epigenome editing	73
Eli Lilly in $650M pact to boost muscle	73
Gabriele et al. reply	72
Uniform volumetric single-cell processing for organ-scale molecular phenotyping	72
Prime editing	72
Starving tumors with fat	71
Linking CRISPR–Cas9 double-strand break profiles to gene editing precision with BreakTag	70
How to grow cement	69
Fresh from the biotech pipeline: FDA approvals settle in 2024, but what next?	69
A germ-free humanized mouse model shows the contribution of resident microbiota to human-specific pathogen infection	69
The reverse transcriptase domain of prime editors contributes to DNA repair in mammalian cells	68
A resource of RNA-binding protein motifs across eukaryotes reveals evolutionary dynamics and gene-regulatory function	68
Single-molecule live-cell RNA imaging with CRISPR–Csm	67
Deep tissue multi-photon imaging using adaptive optics with direct focus sensing and shaping	67
Bio-leather gears up to wow fashion industry	67
Lipid nanoparticle-mediated mRNA delivery to CD34+ cells in rhesus monkeys	66
Generation of a live attenuated influenza A vaccine by proteolysis targeting	66
Make science disruptive again	66
Curated variation benchmarks for challenging medically relevant autosomal genes	66
Scalable single-cell RNA sequencing from full transcripts with Smart-seq3xpress	65
RNA interference in the era of nucleic acid therapeutics	65
Wearable ultrasound for continuous deep-tissue monitoring	65
Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases	65
Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity	64
Challenges for developing broad-based mucosal vaccines for respiratory viruses	64
Ultra-fast label-free quantification and comprehensive proteome coverage with narrow-window data-independent acquisition	63
De novo detection of somatic mutations in high-throughput single-cell profiling data sets	63
Capturing the diversity of protein modifications on presented tumor antigens	63
High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails	63
The challenges and promise of sweat sensing	63
Biotech news from around the world	62
A highly photostable and bright green fluorescent protein	62
Second-quarter biotech job picture	62
Public biotech in 2021 — the numbers	62
Biotech news from around the world	61
Visualizing protein nanostructures in intact brain	61
Forum: Locke and Sykes	60
Biomedical imaging	59
First in vivo base editing lowers cholesterol	58
A retrotransposon for site-specific gene transfer	58
Wasp gut microbes yield beer with extra tang	58
The long game of tenure	58
Italy tests first gene-edited vines for winemaking	57
Radiopharmaceuticals	57
MicroRNAs	57
Fluorescence imaging of RNA and protein in deep tissue	57
Ocean-grown bioplastics	57
Insulin — the new battleground for drug pricing	55
For hemophilia and thalassemia, a new era of ‘one-and-done’ gene therapies has arrived	55