Nature Biotechnology

Article	Citations
Single-cell RNA-seq keeps cells alive	2752
Fungal architecture is a thing, and it’s eye-catching	2066
Fluorescence microscopy resolution increases to near-atomic level	1561
Synthetic biologics provide a path to target bHLH transcription factors	1343
AI’s plastic recycling	884
Author Correction: LAMP-Seq enables sensitive, multiplexed COVID-19 diagnostics using molecular barcoding	827
Integrating medicine, engineering and business to educate early-stage researchers in cardiovascular device development	731
People	728
TRGT-ing the dark genome to accurately characterize tandem repeats at scale	681
Denmark invests in mucosal vaccines	609
Wireless microelectronic fibers to discover gut and brain pathways	601
Overcoming unpredictability in biotech patents by tailored claiming aligned with genuine enablement	583
Drug pipeline 1Q22 — a raft of new modalities … and clinical blowups	549
Framework for predicting alloreactivity in hematopoietic cell transplants	464
Bite-sized solutions	429
An optimized toolkit for prime editing	409
People	405
Behind the graduate mental health crisis in science	382
Chem-map profiles drug binding to chromatin in cells	376
Enhancing prime editing in hematopoietic stem and progenitor cells by modulating nucleotide metabolism	374
The lysosomal degraders	373
Author Correction: Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion	361
Generative models for protein structures and sequences	357
Predicting transcriptional outcomes of novel multigene perturbations with GEARS	343
In vivo development of immune tissue in human intestinal organoids transplanted into humanized mice	338

Engineering innate immune cells for cancer immunotherapy	329
Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome	325
High-efficiency transgene integration by homology-directed repair in human primary cells using DNA-PKcs inhibition	309
Orchestration of secondary metabolite production in Streptomyces species	305
Engineered serum markers for non-invasive monitoring of gene expression in the brain	299
Automated reconstruction of whole-embryo cell lineages by learning from sparse annotations	294
Cellular shuttles put mislocalized proteins in their right place	287
Diffusion model expands RoseTTAFold’s power	285
A competitive precision CRISPR method to identify the fitness effects of transcription factor binding sites	279
Biopharma dives into tumor-seeking radioactive drugs	270
Droplet-based transcriptome profiling of individual synapses	263
Safer non-viral DNA delivery using lipid nanoparticles loaded with endogenous anti-inflammatory lipids	251
Cancer Moonshot aims at liquid biopsies	247
Dreaming ideal protein structures	242
Design of optimized epigenetic regulators for durable gene silencing with application to PCSK9 in nonhuman primates	232
Reflective multi-immersion microscope objectives inspired by the Schmidt telescope	216
Programmable promoter editing for precise control of transgene expression	214
Dual SORT LNPs for multi-organ base editing	211
The world’s first CRISPR therapy is approved: who will receive it?	208
Democratizing knowledge representation with BioCypher	202
Variant to function mapping at single-cell resolution through network propagation	201
An oncolytic virus–T cell chimera for cancer immunotherapy	201
Multi-omics single-cell data integration and regulatory inference with graph-linked embedding	199
Artificial intelligence-guided design of lipid nanoparticles for pulmonary gene therapy	199
Single-cell nanobody-based profiles of multiple epigenetic modalities and chromatin velocity	197
AI predicts cancer relapse from slides	195
Live cell imaging of signaling networks using a conventional microscope	190
Biotech news from around the world	186
3Q22 — more malaise in the markets	183
The need for need-finding in medical education	182
Author Correction: The cost of drug patent expiration date errors	180
Biotech news from around the world	179
Gains in early-onset dementia with progranulin open new paths for drug discovery	178
People	178
HSV-1’s contribution as a vector for gene therapy	177
Who will oversee the ethical limits of human embryo research?	175
Retraction Note: Rescue of the spinal muscular atrophy phenotype in a mouse model by early postnatal delivery of SMN	174
Slaughter-free meat hits the grocery shelf	169
Cancer diagnostics	168
Towards the non-invasive imaging of brain networks and functions at high resolution	167
Delix Therapeutics : psychedelics without the trip	167
Adrestia Therapeutics — Gene networks to the rescue	166
Computationally designed enzymes show potent catalytic activity	166
Precise DNA cleavage using CRISPR-SpRYgests	165
Decoding gene regulation with CRISPR perturbations	165
Orna Therapeutics: circular logic	164
Assessing workforce needs for the emerging CAR-T cell therapy industry	164
Hallucinating functional protein sequences	163
Next-generation psychedelics: should new agents skip the trip?	163
Engineered phage with antibacterial CRISPR–Cas selectively reduce E. coli burden in mice	159

Delivering 3 billion doses of Comirnaty in 2021	159
FDA approves first MASH drug	158
Connecting research and clinical practice: training the next generation of translational scientists	157
Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes	157
Grand challenges in bioinformatics education and training	157
Mesenchymal thymic niche cells enable regeneration of the adult thymus and T cell immunity	154
A language model beats alphafold2 on orphans	153
Protection of cell therapeutics from antibody-mediated killing by CD64 overexpression	153
Prediction of peptide mass spectral libraries with machine learning	153
Detection of mosaic and population-level structural variants with Sniffles2	153
Large-scale discovery of chromatin dysregulation induced by oncofusions and other protein-coding variants	151
A strategy to load, rethread and read protein sequences through a nanopore	150
2022 – toughing out the trough	148
Drug pipeline 4Q22 — sticking around	147
Improving non-viral knock-in with modified single-stranded DNAs and small molecules	146
Top 20 translational researchers of 2020	146
Chemigenetic kinase biosensors illuminate cell signaling networks	146
Baby’s first genome	144
A circularly permuted CasRx platform for efficient, site-specific RNA editing	143
Biotech news from around the world	142
100,000 whole-genome sequences’ diagnostic bonus	141
Forum: Boyden and Danzl	141
Growth of biotech clusters over several decades through pioneering, variety and entrepreneurial science	140
Lab-made antibody stops malaria	140
Microbiome-friendly phages join the campaign for better antimicrobials	139
The devolution of biosimilars regulations	136
Are ecological processes that select beneficial traits in agricultural microbes nature’s intellectual property rights?	135
Control of mammalian gene expression by modulation of polyA signal cleavage at 5′ UTR	133
Rapid, scalable assessment of SARS-CoV-2 cellular immunity by whole-blood PCR	132
Genome-edited farm animals from haploid stem cells	132
COVID vaccine IP waiver agreed	131
Moderna feud with NIH over COVID vaccine	130
Data sharing in the age of deep learning	128
Enzymatic synthesis of RNA oligonucleotides	126
How will generative AI disrupt data science in drug discovery?	126
Consternation follows Marks’ FDA departure	126
Engineering pattern recognition receptors facilitates plant resistance breeding	124
Epicardioid single-cell genomics uncovers principles of human epicardium biology in heart development and disease	122
Spatial host–microbiome sequencing reveals niches in the mouse gut	120
Modular cytokine receptor-targeting chimeras for targeted degradation of cell surface and extracellular proteins	118
Platform solutions for commercial challenges to expanding patient access and making gene editing sustainable	118
Prediction of prime editing insertion efficiencies using sequence features and DNA repair determinants	116
Precision RNA base editing with engineered and endogenous effectors	114
Genome engineering with Cas9 and AAV repair templates generates frequent concatemeric insertions of viral vectors	114
A fully integrated wearable ultrasound system to monitor deep tissues in moving subjects	111
Tension-tuned receptors for synthetic mechanotransduction and intercellular force detection	111
Contamination source modeling with SCRuB improves cancer phenotype prediction from microbiome data	110
CoSpar identifies early cell fate biases from single-cell transcriptomic and lineage information	110
Antivirals	108
Base editors	107
Forum: Drug delivery roundtable with Langer and Whitehead	106
Author Correction: Bioinstructive implantable scaffolds for rapid in vivo manufacture and release of CAR-T cells	106
Publisher Correction: Treg engineers take aim at autoimmunity	106
The strength and importance of government-funded patents for approved drugs	104
2024: research in review	103
Sensitive detection of gene transfer in a microbial community	103
Stem cells	103
People	102
Ionis wins FDA nod for triglyceride-lowering rare disease drug	101
An organoid model with all three pancreatic lineages resembles fetal pancreas	100
Reply to: Methodological concerns and lack of evidence for single-synapse RNA-seq	100
Relieving patent-eligibility barriers in biotech with a preparation or treatment method	100
A genome-wide view of disordered proteins	98
CRISPR beef cattle get FDA green light	97
Thinking about a move from academia to industry? Look before you leap	97
Spotlight on cancer immunotherapies	97
Senolytics under scrutiny in the quest to slow aging	96
Next-generation patient models for colorectal cancer research	95
Author Correction: Precise, predictable multi-nucleotide deletions in rice and wheat using APOBEC–Cas9	95
Tracking gene order provides a new perspective on intraspecific evolution in microbiotas	93
Intestinal mucosal barrier repair and immune regulation with an AI-developed gut-restricted PHD inhibitor	93
Accurate somatic small variant discovery for multiple sequencing technologies with DeepSomatic	93
Children with rare genetic diseases get CRISPR Cures center	92
A community effort to optimize sequence-based deep learning models of gene regulation	92
Five questions with César de la Fuente	92
Generation of accurate, expandable phylogenomic trees with uDance	91
Genotoxins from gut bacteria	90
Conformation-locking antibodies for the discovery and characterization of KRAS inhibitors	90
High-precision cytosine base editors by evolving nucleic-acid-recognition hotspots in deaminase	90
Systema: a framework for evaluating genetic perturbation response prediction beyond systematic variation	89

Septerna: making another run on GPCRs	89
High-throughput discovery of MHC class I- and II-restricted T cell epitopes using synthetic cellular circuits	88
Multifunctional microelectronic fibers enable wireless modulation of gut and brain neural circuits	88
Facile repurposing of peptide–MHC-restricted antibodies for cancer immunotherapy	87
Precise integration of large DNA sequences in plant genomes using PrimeRoot editors	87
Author Correction: Engineering circular RNA for enhanced protein production	87
How will AI affect patent disclosures?	87
Accelerated identification of disease-causing variants with ultra-rapid nanopore genome sequencing	87
Discovery of drug–omics associations in type 2 diabetes with generative deep-learning models	87
Human respiratory airway progenitors derived from pluripotent cells generate alveolar epithelial cells and model pulmonary fibrosis	87
Mapping medically relevant RNA isoform diversity in the aged human frontal cortex with deep long-read RNA-seq	86
Tuning plant phenotypes by precise, graded downregulation of gene expression	86
The GA4GH Phenopacket schema defines a computable representation of clinical data	85
Spatial genomics of AAV vectors reveals mechanism of transcriptional crosstalk that enables targeted delivery of large genetic cargo	84
Post-translational modifications reshape the antigenic landscape of the MHC I immunopeptidome in tumors	84
Efficient prime editing in mouse brain, liver and heart with dual AAVs	84
Hypoimmune induced pluripotent stem cells survive long term in fully immunocompetent, allogeneic rhesus macaques	83
AI-enhanced protein design makes proteins that have never existed	83
TCR cell therapies vanquish solid tumors — finally	83
3Q23 — VCs feed ‘older children’ as IPO markets stagnate	82
Fashion’s microbial dyeing machines	82
Biotech news from around the world	81
People	81
Measuring the impact of chromatin context on transcription factor binding affinities	81
Starving tumors with fat	81
Clarifying US regulations on xenotransplantation	80
2H23 biotech job picture	80
Base editor restores CD3δ-dependent SCID mutation	79
Microbiomics	79
Microfluidics	79
Bridge RNAs direct programmable DNA rearrangements	78
Discovering the crucial function of long noncoding RNAs	78
Publisher Correction: Top 20 translational researchers of 2020	78
The instant spray-on dress	78
Redefining antibody patent protection using paratope mapping and CDR-scanning	77
Startup grows egg proteins in potato fields	77
Biotech patenting 2021	77
Big pharma seduced by transcription factors again. What has changed?	76
Embryonic stem cells across bird species	76
Combining reference genomes into a pangenome graph improves accuracy and reduces bias	76
How to grow cement	75
Lipid nanoparticle-mediated mRNA delivery to CD34+ cells in rhesus monkeys	75
Using artificial intelligence to develop gene therapy for the lungs	74
Eli Lilly in $650M pact to boost muscle	74
The DxConnect Virtual Biobank connects diagnostic researchers to clinical samples	74
Resurrecting a miniature Cas9 ancestor for genome and epigenome editing	73
Ultra-fast label-free quantification and comprehensive proteome coverage with narrow-window data-independent acquisition	73
Capturing the diversity of protein modifications on presented tumor antigens	73
Curated variation benchmarks for challenging medically relevant autosomal genes	72
Wearable ultrasound for continuous deep-tissue monitoring	72
Challenges for developing broad-based mucosal vaccines for respiratory viruses	72
A germ-free humanized mouse model shows the contribution of resident microbiota to human-specific pathogen infection	71
De novo detection of somatic mutations in high-throughput single-cell profiling data sets	71
Generation of a live attenuated influenza A vaccine by proteolysis targeting	71
Combination therapy patents: a new front in evergreening	71
Prime editing	70
A shifting market environment and strategies fueling innovation in prostate cancer treatment	69
Linking CRISPR–Cas9 double-strand break profiles to gene editing precision with BreakTag	69
A resource of RNA-binding protein motifs across eukaryotes reveals evolutionary dynamics and gene-regulatory function	68
Bio-leather gears up to wow fashion industry	68
Uniform volumetric single-cell processing for organ-scale molecular phenotyping	67
Make science disruptive again	67
Fighting fibrosis	67
The reverse transcriptase domain of prime editors contributes to DNA repair in mammalian cells	66
Fresh from the biotech pipeline: FDA approvals settle in 2024, but what next?	66
Deep tissue multi-photon imaging using adaptive optics with direct focus sensing and shaping	66
High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails	66
Scalable single-cell RNA sequencing from full transcripts with Smart-seq3xpress	65
Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity	64
Chikungunya vaccine approved	63
Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases	62
A highly photostable and bright green fluorescent protein	62
Biotech news from around the world	61
RNA interference in the era of nucleic acid therapeutics	61
Second-quarter biotech job picture	61
The challenges and promise of sweat sensing	61
Public biotech in 2021 — the numbers	61
Single-molecule live-cell RNA imaging with CRISPR–Csm	61
Biotech news from around the world	60
Visualizing protein nanostructures in intact brain	60
Wasp gut microbes yield beer with extra tang	59
First in vivo base editing lowers cholesterol	59
The long game of tenure	59
Biomedical imaging	59
Radiopharmaceuticals	59
A retrotransposon for site-specific gene transfer	59
Forum: Locke and Sykes	59
RIP-PEN-seq identifies a class of kink-turn RNAs as splicing regulators	58
Ocean-grown bioplastics	58
MicroRNAs	58
Italy tests first gene-edited vines for winemaking	57
For hemophilia and thalassemia, a new era of ‘one-and-done’ gene therapies has arrived	57
CRISPR comes a-knock-in to reprogram antibodies in vivo	57
A way forward for diagnosis of patients with extremely rare genetic mutations	56
Fluorescence imaging of RNA and protein in deep tissue	56