Nature Biotechnology

Article	Citations
Framework for predicting alloreactivity in hematopoietic cell transplants	1706
TRGT-ing the dark genome to accurately characterize tandem repeats at scale	1664
The champions of drug development	1138
People	1081
Cancer Moonshot aims at liquid biopsies	729
Author Correction: LAMP-Seq enables sensitive, multiplexed COVID-19 diagnostics using molecular barcoding	644
Integrating medicine, engineering and business to educate early-stage researchers in cardiovascular device development	585
AI’s plastic recycling	578
Fluorescence microscopy resolution increases to near-atomic level	577
Single-cell RNA-seq keeps cells alive	549
Ultra-precision medicine	499
Diffusion model expands RoseTTAFold’s power	496
Fungal architecture is a thing, and it’s eye-catching	479
Behind the graduate mental health crisis in science	461
Dreaming ideal protein structures	434
Synthetic biologics provide a path to target bHLH transcription factors	399
Author Correction: An injectable bone marrow–like scaffold enhances T cell immunity after hematopoietic stem cell transplantation	398
An optimized toolkit for prime editing	387
Overcoming unpredictability in biotech patents by tailored claiming aligned with genuine enablement	370
Negative innovation: when patents are bad for patients	368
Drug pipeline 1Q22 — a raft of new modalities … and clinical blowups	331
Denmark invests in mucosal vaccines	324
Wireless microelectronic fibers to discover gut and brain pathways	318
People	314
Bite-sized solutions	308

Author Correction: Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion	304
Cellular shuttles put mislocalized proteins in their right place	304
The world’s first CRISPR therapy is approved: who will receive it?	297
The lysosomal degraders	287
A competitive precision CRISPR method to identify the fitness effects of transcription factor binding sites	276
Chem-map profiles drug binding to chromatin in cells	276
Biopharma dives into tumor-seeking radioactive drugs	274
Enhancing prime editing in hematopoietic stem and progenitor cells by modulating nucleotide metabolism	267
Democratizing knowledge representation with BioCypher	261
Variant to function mapping at single-cell resolution through network propagation	260
An oncolytic virus–T cell chimera for cancer immunotherapy	255
Engineered serum markers for non-invasive monitoring of gene expression in the brain	254
Safer non-viral DNA delivery using lipid nanoparticles loaded with endogenous anti-inflammatory lipids	245
A small and highly sensitive red/far-red optogenetic switch for applications in mammals	245
Engineering innate immune cells for cancer immunotherapy	240
Reflective multi-immersion microscope objectives inspired by the Schmidt telescope	231
High-efficiency transgene integration by homology-directed repair in human primary cells using DNA-PKcs inhibition	223
Ebola as a case study for the patent landscape of medical countermeasures for emerging infectious diseases	222
Automated reconstruction of whole-embryo cell lineages by learning from sparse annotations	221
In vivo development of immune tissue in human intestinal organoids transplanted into humanized mice	217
Generative models for protein structures and sequences	214
Artificial intelligence-guided design of lipid nanoparticles for pulmonary gene therapy	213
Predicting transcriptional outcomes of novel multigene perturbations with GEARS	203
Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome	200
Droplet-based transcriptome profiling of individual synapses	195
Multi-omics single-cell data integration and regulatory inference with graph-linked embedding	195
3Q22 — more malaise in the markets	194
Biotech news from around the world	189
Light-sheet microscopy at high resolution	188
HSV-1’s contribution as a vector for gene therapy	177
The need for need-finding in medical education	176
Hallucinating functional protein sequences	176
Live cell imaging of signaling networks using a conventional microscope	173
Author Correction: The cost of drug patent expiration date errors	173
Single-cell nanobody-based profiles of multiple epigenetic modalities and chromatin velocity	170
AI predicts cancer relapse from slides	165
Delix Therapeutics : psychedelics without the trip	165
Towards the non-invasive imaging of brain networks and functions at high resolution	164
Biotech news from around the world	163
Slaughter-free meat hits the grocery shelf	162
People	162
Adrestia Therapeutics — Gene networks to the rescue	159
Cancer diagnostics	157
Next-generation psychedelics: should new agents skip the trip?	154
Grand challenges in bioinformatics education and training	154
Who will oversee the ethical limits of human embryo research?	153
Gains in early-onset dementia with progranulin open new paths for drug discovery	152
Delivering 3 billion doses of Comirnaty in 2021	152
Decoding gene regulation with CRISPR perturbations	151
Retraction Note: Rescue of the spinal muscular atrophy phenotype in a mouse model by early postnatal delivery of SMN	150

Orna Therapeutics: circular logic	150
A language model beats alphafold2 on orphans	149
Protection of cell therapeutics from antibody-mediated killing by CD64 overexpression	149
FDA approves first MASH drug	147
Precise DNA cleavage using CRISPR-SpRYgests	145
Prediction of peptide mass spectral libraries with machine learning	144
Engineered phage with antibacterial CRISPR–Cas selectively reduce E. coli burden in mice	142
Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes	142
Mapping single-cell data to reference atlases by transfer learning	141
Nanotechnology offers alternative ways to fight COVID-19 pandemic with antivirals	141
Detection of mosaic and population-level structural variants with Sniffles2	140
Engineered pegRNAs improve prime editing efficiency	139
Improving non-viral knock-in with modified single-stranded DNAs and small molecules	137
Assessing workforce needs for the emerging CAR-T cell therapy industry	137
2022 – toughing out the trough	137
Biotech news from around the world	134
Drug pipeline 4Q22 — sticking around	134
Top 20 translational researchers of 2020	133
CoSpar identifies early cell fate biases from single-cell transcriptomic and lineage information	132
Microbiome-friendly phages join the campaign for better antimicrobials	131
Chemigenetic kinase biosensors illuminate cell signaling networks	129
A carte blanche approval in Alzheimer’s	128
Forum: Boyden and Danzl	127
Microbiomes	127
Biotech news from around the world	127
100,000 whole-genome sequences’ diagnostic bonus	127
COVID vaccine IP waiver agreed	126
Synthetic virology: the experts speak	126
Lab-made antibody stops malaria	126
Are ecological processes that select beneficial traits in agricultural microbes nature’s intellectual property rights?	124
Rapid, scalable assessment of SARS-CoV-2 cellular immunity by whole-blood PCR	124
Moderna feud with NIH over COVID vaccine	124
Growth of biotech clusters over several decades through pioneering, variety and entrepreneurial science	123
Large-scale discovery of chromatin dysregulation induced by oncofusions and other protein-coding variants	123
The devolution of biosimilars regulations	120
A strategy to load, rethread and read protein sequences through a nanopore	119
Genome engineering with Cas9 and AAV repair templates generates frequent concatemeric insertions of viral vectors	117
Precision RNA base editing with engineered and endogenous effectors	117
How will generative AI disrupt data science in drug discovery?	115
Consternation follows Marks’ FDA departure	115
Prediction of prime editing insertion efficiencies using sequence features and DNA repair determinants	115
Enzymatic synthesis of RNA oligonucleotides	114
A circularly permuted CasRx platform for efficient, site-specific RNA editing	114
Baby’s first genome	113
Contamination source modeling with SCRuB improves cancer phenotype prediction from microbiome data	112
Spatial host–microbiome sequencing reveals niches in the mouse gut	112
Data sharing in the age of deep learning	111
Tension-tuned receptors for synthetic mechanotransduction and intercellular force detection	110
Chromatin Velocity reveals epigenetic dynamics by single-cell profiling of heterochromatin and euchromatin	110
Modular cytokine receptor-targeting chimeras for targeted degradation of cell surface and extracellular proteins	107
Epicardioid single-cell genomics uncovers principles of human epicardium biology in heart development and disease	107
Control of mammalian gene expression by modulation of polyA signal cleavage at 5′ UTR	106
Author Correction: Bioinstructive implantable scaffolds for rapid in vivo manufacture and release of CAR-T cells	106
A fully integrated wearable ultrasound system to monitor deep tissues in moving subjects	106
Base editors	105
Publisher Correction: Treg engineers take aim at autoimmunity	105
Antivirals	105
Relieving patent-eligibility barriers in biotech with a preparation or treatment method	103
Forum: Drug delivery roundtable with Langer and Whitehead	102
How will AI affect patent disclosures?	100
Ionis wins FDA nod for triglyceride-lowering rare disease drug	100
Five questions with César de la Fuente	99
An organoid model with all three pancreatic lineages resembles fetal pancreas	99
2024: research in review	98
Publisher Correction: Questions swirl around failures of disease-modifying Huntington’s drugs	97
Stem cells	97
People	96
Publisher Correction: Send in the senolytics	96
Author Correction: Engineering circular RNA for enhanced protein production	95
Sensitive detection of gene transfer in a microbial community	95
A genome-wide view of disordered proteins	94
Human respiratory airway progenitors derived from pluripotent cells generate alveolar epithelial cells and model pulmonary fibrosis	94
Reply to: Methodological concerns and lack of evidence for single-synapse RNA-seq	94
Conformation-locking antibodies for the discovery and characterization of KRAS inhibitors	93
Septerna: making another run on GPCRs	93
TCR cell therapies vanquish solid tumors — finally	92
Spotlight on cancer immunotherapies	92
Author Correction: Precise, predictable multi-nucleotide deletions in rice and wheat using APOBEC–Cas9	91
Genotoxins from gut bacteria	91
Facile repurposing of peptide–MHC-restricted antibodies for cancer immunotherapy	90

CRISPR beef cattle get FDA green light	90
High-throughput discovery of MHC class I- and II-restricted T cell epitopes using synthetic cellular circuits	88
A community effort to optimize sequence-based deep learning models of gene regulation	87
Purification of multiplex oligonucleotide libraries by synthesis and selection	87
Integration of spatial and single-cell transcriptomic data elucidates mouse organogenesis	86
Multifunctional microelectronic fibers enable wireless modulation of gut and brain neural circuits	86
Accelerated identification of disease-causing variants with ultra-rapid nanopore genome sequencing	86
Hypoimmune induced pluripotent stem cells survive long term in fully immunocompetent, allogeneic rhesus macaques	85
Tracking gene order provides a new perspective on intraspecific evolution in microbiotas	84
Generation of accurate, expandable phylogenomic trees with uDance	84
Next-generation patient models for colorectal cancer research	83
Efficient prime editing in mouse brain, liver and heart with dual AAVs	82
Post-translational modifications reshape the antigenic landscape of the MHC I immunopeptidome in tumors	82
AI-enhanced protein design makes proteins that have never existed	82
Fully implantable and bioresorbable cardiac pacemakers without leads or batteries	81
The GA4GH Phenopacket schema defines a computable representation of clinical data	81
Intestinal mucosal barrier repair and immune regulation with an AI-developed gut-restricted PHD inhibitor	80
Mapping medically relevant RNA isoform diversity in the aged human frontal cortex with deep long-read RNA-seq	80
Precise integration of large DNA sequences in plant genomes using PrimeRoot editors	80
Tuning plant phenotypes by precise, graded downregulation of gene expression	79
Spatial genomics of AAV vectors reveals mechanism of transcriptional crosstalk that enables targeted delivery of large genetic cargo	79
Discovery of drug–omics associations in type 2 diabetes with generative deep-learning models	79
2H23 biotech job picture	78
Combining reference genomes into a pangenome graph improves accuracy and reduces bias	78
3Q23 — VCs feed ‘older children’ as IPO markets stagnate	78
Clarifying US regulations on xenotransplantation	78
People	77
Starving tumors with fat	77
Base editor restores CD3δ-dependent SCID mutation	76
Measuring the impact of chromatin context on transcription factor binding affinities	76
Fashion’s microbial dyeing machines	76
Biotech news from around the world	76
Microfluidics	75
Microbiomics	74
Bridge RNAs direct programmable DNA rearrangements	73
The instant spray-on dress	73
Redefining antibody patent protection using paratope mapping and CDR-scanning	72
Biotech patenting 2021	72
Startup grows egg proteins in potato fields	72
Capturing the diversity of protein modifications on presented tumor antigens	71
Fighting fibrosis	71
A shifting market environment and strategies fueling innovation in prostate cancer treatment	71
How to grow cement	71
Publisher Correction: Top 20 translational researchers of 2020	71
Chikungunya vaccine approved	71
Discovering the crucial function of long noncoding RNAs	71
Prime editing	70
The reverse transcriptase domain of prime editors contributes to DNA repair in mammalian cells	70
Uniform volumetric single-cell processing for organ-scale molecular phenotyping	70
Make science disruptive again	70
Single-molecule live-cell RNA imaging with CRISPR–Csm	69
Bio-leather gears up to wow fashion industry	69
Bioorthogonal information storage in l-DNA with a high-fidelity mirror-image Pfu DNA polymerase	69
Wearable ultrasound for continuous deep-tissue monitoring	69
Challenges for developing broad-based mucosal vaccines for respiratory viruses	68
The DxConnect Virtual Biobank connects diagnostic researchers to clinical samples	68
Fresh from the biotech pipeline: FDA approvals settle in 2024, but what next?	68
A germ-free humanized mouse model shows the contribution of resident microbiota to human-specific pathogen infection	68
Scalable single-cell RNA sequencing from full transcripts with Smart-seq3xpress	67
De novo detection of somatic mutations in high-throughput single-cell profiling data sets	67
Generation of a live attenuated influenza A vaccine by proteolysis targeting	67
Curated variation benchmarks for challenging medically relevant autosomal genes	67
Using artificial intelligence to develop gene therapy for the lungs	66
A highly photostable and bright green fluorescent protein	66
Ultra-fast label-free quantification and comprehensive proteome coverage with narrow-window data-independent acquisition	66
The challenges and promise of sweat sensing	66
Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity	66
Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases	66
Combination therapy patents: a new front in evergreening	66
Linking CRISPR–Cas9 double-strand break profiles to gene editing precision with BreakTag	65
Deep tissue multi-photon imaging using adaptive optics with direct focus sensing and shaping	64
RNA interference in the era of nucleic acid therapeutics	64
Visualizing protein nanostructures in intact brain	63
High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails	63
Lipid nanoparticle-mediated mRNA delivery to CD34+ cells in rhesus monkeys	63
Biotech news from around the world	62
People	62
Biotech news from around the world	61
Baby genomics: newborn sequencing starts to fulfill its promise	61
Public biotech in 2021 — the numbers	61
Second-quarter biotech job picture	60
Italy tests first gene-edited vines for winemaking	60
People	60
Innovators want pills to treat sickle cell disease. Can they match gene therapy?	60
Biomedical imaging	59
Wireless multisite physiological sensor with clinical-grade accuracy	59
Forum: Locke and Sykes	59
For hemophilia and thalassemia, a new era of ‘one-and-done’ gene therapies has arrived	59
First in vivo base editing lowers cholesterol	59
Author Correction: m6A RNA modifications are measured at single-base resolution across the mammalian transcriptome	58
Insulin — the new battleground for drug pricing	58
The long game of tenure	58
A retrotransposon for site-specific gene transfer	58
Japan embraces CRISPR-edited fish	56
Model-directed generation of artificial CRISPR–Cas13a guide RNA sequences improves nucleic acid detection	56