Nature Biotechnology

Article	Citations
Single-cell RNA-seq keeps cells alive	2488
Fungal architecture is a thing, and it’s eye-catching	1981
Fluorescence microscopy resolution increases to near-atomic level	1455
Synthetic biologics provide a path to target bHLH transcription factors	1300
AI’s plastic recycling	859
Author Correction: LAMP-Seq enables sensitive, multiplexed COVID-19 diagnostics using molecular barcoding	780
Integrating medicine, engineering and business to educate early-stage researchers in cardiovascular device development	709
Cancer Moonshot aims at liquid biopsies	692
People	661
TRGT-ing the dark genome to accurately characterize tandem repeats at scale	582
Denmark invests in mucosal vaccines	559
Wireless microelectronic fibers to discover gut and brain pathways	555
Overcoming unpredictability in biotech patents by tailored claiming aligned with genuine enablement	535
Drug pipeline 1Q22 — a raft of new modalities … and clinical blowups	523
Dreaming ideal protein structures	426
Framework for predicting alloreactivity in hematopoietic cell transplants	413
Bite-sized solutions	391
Engineering innate immune cells for cancer immunotherapy	386
An optimized toolkit for prime editing	368
Author Correction: An injectable bone marrow–like scaffold enhances T cell immunity after hematopoietic stem cell transplantation	368
A small and highly sensitive red/far-red optogenetic switch for applications in mammals	366
Multi-omics single-cell data integration and regulatory inference with graph-linked embedding	352
Safer non-viral DNA delivery using lipid nanoparticles loaded with endogenous anti-inflammatory lipids	350
Orchestration of secondary metabolite production in Streptomyces species	342
Enhancing prime editing in hematopoietic stem and progenitor cells by modulating nucleotide metabolism	332

Engineered serum markers for non-invasive monitoring of gene expression in the brain	330
Democratizing knowledge representation with BioCypher	321
The lysosomal degraders	300
Variant to function mapping at single-cell resolution through network propagation	297
Dual SORT LNPs for multi-organ base editing	297
High-efficiency transgene integration by homology-directed repair in human primary cells using DNA-PKcs inhibition	292
In vivo development of immune tissue in human intestinal organoids transplanted into humanized mice	280
Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome	277
Reflective multi-immersion microscope objectives inspired by the Schmidt telescope	276
People	271
Author Correction: Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion	262
Behind the graduate mental health crisis in science	257
Cellular shuttles put mislocalized proteins in their right place	254
Diffusion model expands RoseTTAFold’s power	244
A competitive precision CRISPR method to identify the fitness effects of transcription factor binding sites	232
Biopharma dives into tumor-seeking radioactive drugs	230
An oncolytic virus–T cell chimera for cancer immunotherapy	211
Chem-map profiles drug binding to chromatin in cells	211
Generative models for protein structures and sequences	210
Automated reconstruction of whole-embryo cell lineages by learning from sparse annotations	201
The world’s first CRISPR therapy is approved: who will receive it?	200
Predicting transcriptional outcomes of novel multigene perturbations with GEARS	197
Droplet-based transcriptome profiling of individual synapses	196
Single-cell nanobody-based profiles of multiple epigenetic modalities and chromatin velocity	195
Artificial intelligence-guided design of lipid nanoparticles for pulmonary gene therapy	195
AI predicts cancer relapse from slides	194
Biotech news from around the world	190
Live cell imaging of signaling networks using a conventional microscope	190
3Q22 — more malaise in the markets	187
Light-sheet microscopy at high resolution	186
The need for need-finding in medical education	184
Author Correction: The cost of drug patent expiration date errors	183
Biotech news from around the world	179
People	175
Gains in early-onset dementia with progranulin open new paths for drug discovery	173
HSV-1’s contribution as a vector for gene therapy	172
Who will oversee the ethical limits of human embryo research?	172
Next-generation psychedelics: should new agents skip the trip?	171
Retraction Note: Rescue of the spinal muscular atrophy phenotype in a mouse model by early postnatal delivery of SMN	171
Slaughter-free meat hits the grocery shelf	171
Assessing workforce needs for the emerging CAR-T cell therapy industry	170
Cancer diagnostics	169
Hallucinating functional protein sequences	169
Delix Therapeutics : psychedelics without the trip	165
Towards the non-invasive imaging of brain networks and functions at high resolution	163
Computationally designed enzymes show potent catalytic activity	162
FDA approves first MASH drug	162
Grand challenges in bioinformatics education and training	162
Decoding gene regulation with CRISPR perturbations	160
Adrestia Therapeutics — Gene networks to the rescue	160

A language model beats alphafold2 on orphans	159
Nanotechnology offers alternative ways to fight COVID-19 pandemic with antivirals	159
Engineered phage with antibacterial CRISPR–Cas selectively reduce E. coli burden in mice	156
Orna Therapeutics: circular logic	156
Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes	155
Protection of cell therapeutics from antibody-mediated killing by CD64 overexpression	154
Delivering 3 billion doses of Comirnaty in 2021	152
Precise DNA cleavage using CRISPR-SpRYgests	152
Engineered pegRNAs improve prime editing efficiency	151
Detection of mosaic and population-level structural variants with Sniffles2	151
Prediction of peptide mass spectral libraries with machine learning	151
Large-scale discovery of chromatin dysregulation induced by oncofusions and other protein-coding variants	150
A strategy to load, rethread and read protein sequences through a nanopore	149
Drug pipeline 4Q22 — sticking around	148
2022 – toughing out the trough	148
Chemigenetic kinase biosensors illuminate cell signaling networks	147
Improving non-viral knock-in with modified single-stranded DNAs and small molecules	147
Top 20 translational researchers of 2020	146
Baby’s first genome	146
Platform solutions for commercial challenges to expanding patient access and making gene editing sustainable	145
A circularly permuted CasRx platform for efficient, site-specific RNA editing	145
Biotech news from around the world	144
100,000 whole-genome sequences’ diagnostic bonus	141
Lab-made antibody stops malaria	139
Forum: Boyden and Danzl	139
Growth of biotech clusters over several decades through pioneering, variety and entrepreneurial science	138
The devolution of biosimilars regulations	138
Are ecological processes that select beneficial traits in agricultural microbes nature’s intellectual property rights?	137
Control of mammalian gene expression by modulation of polyA signal cleavage at 5′ UTR	136
COVID vaccine IP waiver agreed	134
Synthetic virology: the experts speak	132
Moderna feud with NIH over COVID vaccine	132
Enzymatic synthesis of RNA oligonucleotides	131
Consternation follows Marks’ FDA departure	131
Prediction of prime editing insertion efficiencies using sequence features and DNA repair determinants	129
Rapid, scalable assessment of SARS-CoV-2 cellular immunity by whole-blood PCR	128
Precision RNA base editing with engineered and endogenous effectors	128
Microbiome-friendly phages join the campaign for better antimicrobials	125
Data sharing in the age of deep learning	124
Genome engineering with Cas9 and AAV repair templates generates frequent concatemeric insertions of viral vectors	124
Spatial host–microbiome sequencing reveals niches in the mouse gut	123
Tension-tuned receptors for synthetic mechanotransduction and intercellular force detection	122
CoSpar identifies early cell fate biases from single-cell transcriptomic and lineage information	121
Epicardioid single-cell genomics uncovers principles of human epicardium biology in heart development and disease	117
A fully integrated wearable ultrasound system to monitor deep tissues in moving subjects	117
Chromatin Velocity reveals epigenetic dynamics by single-cell profiling of heterochromatin and euchromatin	117
How will generative AI disrupt data science in drug discovery?	114
Contamination source modeling with SCRuB improves cancer phenotype prediction from microbiome data	113
Modular cytokine receptor-targeting chimeras for targeted degradation of cell surface and extracellular proteins	112
Antivirals	112
Publisher Correction: Treg engineers take aim at autoimmunity	110
Base editors	110
Forum: Drug delivery roundtable with Langer and Whitehead	108
Author Correction: Bioinstructive implantable scaffolds for rapid in vivo manufacture and release of CAR-T cells	108
Systema: a framework for evaluating genetic perturbation response prediction beyond systematic variation	106
The strength and importance of government-funded patents for approved drugs	106
Sensitive detection of gene transfer in a microbial community	105
TCR cell therapies vanquish solid tumors — finally	104
2024: research in review	104
People	104
Stem cells	104
Ionis wins FDA nod for triglyceride-lowering rare disease drug	103
How will AI affect patent disclosures?	102
An organoid model with all three pancreatic lineages resembles fetal pancreas	100
Relieving patent-eligibility barriers in biotech with a preparation or treatment method	100
A genome-wide view of disordered proteins	99
Reply to: Methodological concerns and lack of evidence for single-synapse RNA-seq	99
Author Correction: Engineering circular RNA for enhanced protein production	98
CRISPR beef cattle get FDA green light	97
Spotlight on cancer immunotherapies	96
Thinking about a move from academia to industry? Look before you leap	96
Septerna: making another run on GPCRs	95
Genotoxins from gut bacteria	95
Senolytics under scrutiny in the quest to slow aging	95
Author Correction: Precise, predictable multi-nucleotide deletions in rice and wheat using APOBEC–Cas9	94
Next-generation patient models for colorectal cancer research	92
Tracking gene order provides a new perspective on intraspecific evolution in microbiotas	92
A community effort to optimize sequence-based deep learning models of gene regulation	91
Tuning plant phenotypes by precise, graded downregulation of gene expression	90
Facile repurposing of peptide–MHC-restricted antibodies for cancer immunotherapy	90

Intestinal mucosal barrier repair and immune regulation with an AI-developed gut-restricted PHD inhibitor	90
Five questions with César de la Fuente	89
High-throughput discovery of MHC class I- and II-restricted T cell epitopes using synthetic cellular circuits	89
AI-enhanced protein design makes proteins that have never existed	89
Human respiratory airway progenitors derived from pluripotent cells generate alveolar epithelial cells and model pulmonary fibrosis	89
Children with rare genetic diseases get CRISPR Cures center	88
Discovery of drug–omics associations in type 2 diabetes with generative deep-learning models	87
Generation of accurate, expandable phylogenomic trees with uDance	87
The GA4GH Phenopacket schema defines a computable representation of clinical data	87
Mapping medically relevant RNA isoform diversity in the aged human frontal cortex with deep long-read RNA-seq	87
Multifunctional microelectronic fibers enable wireless modulation of gut and brain neural circuits	86
High-precision cytosine base editors by evolving nucleic-acid-recognition hotspots in deaminase	86
Spatial genomics of AAV vectors reveals mechanism of transcriptional crosstalk that enables targeted delivery of large genetic cargo	86
Conformation-locking antibodies for the discovery and characterization of KRAS inhibitors	86
Precise integration of large DNA sequences in plant genomes using PrimeRoot editors	84
Accelerated identification of disease-causing variants with ultra-rapid nanopore genome sequencing	84
Hypoimmune induced pluripotent stem cells survive long term in fully immunocompetent, allogeneic rhesus macaques	84
Efficient prime editing in mouse brain, liver and heart with dual AAVs	83
Post-translational modifications reshape the antigenic landscape of the MHC I immunopeptidome in tumors	82
People	82
Fashion’s microbial dyeing machines	82
3Q23 — VCs feed ‘older children’ as IPO markets stagnate	82
Biotech news from around the world	81
Measuring the impact of chromatin context on transcription factor binding affinities	81
2H23 biotech job picture	81
Starving tumors with fat	81
Clarifying US regulations on xenotransplantation	80
Microfluidics	80
Base editor restores CD3δ-dependent SCID mutation	80
Discovering the crucial function of long noncoding RNAs	79
Microbiomics	79
Bridge RNAs direct programmable DNA rearrangements	78
Publisher Correction: Top 20 translational researchers of 2020	78
The instant spray-on dress	78
Redefining antibody patent protection using paratope mapping and CDR-scanning	77
Biotech patenting 2021	76
Deep tissue multi-photon imaging using adaptive optics with direct focus sensing and shaping	76
Startup grows egg proteins in potato fields	76
Scalable single-cell RNA sequencing from full transcripts with Smart-seq3xpress	76
High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails	75
How to grow cement	75
Combining reference genomes into a pangenome graph improves accuracy and reduces bias	75
Single-molecule live-cell RNA imaging with CRISPR–Csm	75
Fighting fibrosis	74
Using artificial intelligence to develop gene therapy for the lungs	74
Lipid nanoparticle-mediated mRNA delivery to CD34+ cells in rhesus monkeys	74
Wearable ultrasound for continuous deep-tissue monitoring	74
Eli Lilly in $650M pact to boost muscle	73
Combination therapy patents: a new front in evergreening	73
The DxConnect Virtual Biobank connects diagnostic researchers to clinical samples	73
De novo detection of somatic mutations in high-throughput single-cell profiling data sets	73
Fresh from the biotech pipeline: FDA approvals settle in 2024, but what next?	73
Uniform volumetric single-cell processing for organ-scale molecular phenotyping	73
Resurrecting a miniature Cas9 ancestor for genome and epigenome editing	72
The reverse transcriptase domain of prime editors contributes to DNA repair in mammalian cells	72
Capturing the diversity of protein modifications on presented tumor antigens	72
Bio-leather gears up to wow fashion industry	72
A resource of RNA-binding protein motifs across eukaryotes reveals evolutionary dynamics and gene-regulatory function	71
A shifting market environment and strategies fueling innovation in prostate cancer treatment	70
Prime editing	70
Chikungunya vaccine approved	70
Linking CRISPR–Cas9 double-strand break profiles to gene editing precision with BreakTag	69
Make science disruptive again	69
Generation of a live attenuated influenza A vaccine by proteolysis targeting	69
The challenges and promise of sweat sensing	68
Embryonic stem cells across bird species	68
Ultra-fast label-free quantification and comprehensive proteome coverage with narrow-window data-independent acquisition	68
Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity	67
Curated variation benchmarks for challenging medically relevant autosomal genes	67
Challenges for developing broad-based mucosal vaccines for respiratory viruses	67
RNA interference in the era of nucleic acid therapeutics	66
Public biotech in 2021 — the numbers	66
A germ-free humanized mouse model shows the contribution of resident microbiota to human-specific pathogen infection	66
A highly photostable and bright green fluorescent protein	66
Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases	66
Biotech news from around the world	65
Second-quarter biotech job picture	65
Visualizing protein nanostructures in intact brain	64
Biotech news from around the world	64
Forum: Locke and Sykes	63
People	63
Biomedical imaging	62
First in vivo base editing lowers cholesterol	61
A retrotransposon for site-specific gene transfer	60
Insulin — the new battleground for drug pricing	60
Wasp gut microbes yield beer with extra tang	59
A multi-kingdom genetic barcoding system for precise clone isolation	59
The long game of tenure	59
Identification of patient-specific CD4+ and CD8+ T cell neoantigens through HLA-unbiased genetic screens	59
Sizing up beta cells made from stem cells	59
Ocean-grown bioplastics	58
The emerging landscape of engineered bacteria cancer therapies	58
Radiopharmaceuticals	58
DARPins score against COVID-19	58
CRISPR comes a-knock-in to reprogram antibodies in vivo	57