Nature Biotechnology

Article	Citations
Forum: Alexandrov and Le	1579
Federation Bio: from single strains to a purely synthetic microbiome	1321
Forum: Optogenetics roundtable with Deisseroth and Gradinaru	1046
Multiplexed mapping of chromatin features at single-cell resolution	926
First Rounders: Jay Keasling	677
Unlocking drug modes of action with multi-dimensional high-throughput metabolic profiling	603
Toward a safer and more secure US bioeconomy	537
Glia-enriched cortical organoids implanted in mice capture astrocyte diversity	531
Making space for spatial biology in the clinic	488
Video game unleashes millions of citizen scientists on microbiome research	465
Synthetic GPCRs for controlling cellular responses	454
Base editors	449
Safer non-viral DNA delivery using lipid nanoparticles loaded with endogenous anti-inflammatory lipids	440
Forum: CRISPR screening roundtable with Stegmaier and Doench	411
People	391
Biotech news from around the world	378
Bilateral China–Brazil ties over agbiotech	374
Bluebird’s CALD gene therapy poised for approval	350
Improving the safety of lipid nanoparticle-based DNA delivery for extended gene expression	347
Biotech news from around the world	333
An oncolytic virus–T cell chimera for cancer immunotherapy	329
Variant to function mapping at single-cell resolution through network propagation	311
Bat biotech takes flight	310
Biotech news from around the world	309
Diffusion model expands RoseTTAFold’s power	301

Fungal architecture is a thing, and it’s eye-catching	287
Engineered Cas13 variants with minimal collateral RNA targeting	286
People	286
Linking genetic variants to cellular function and disease	278
Ultra-precision medicine	272
First Rounders: Lita Nelsen	269
Photys Therapeutics: customizing phosphorylation via molecular matchmaking	269
The champions of drug development	261
Biotech news from around the world	254
Women build strength in numbers	254
Synthetic biologics provide a path to target bHLH transcription factors	250
A neural network for long-term super-resolution imaging of live cells with reliable confidence quantification	244
Increase vigilance against cyberattacks	241
Spatial characterization of single tumor cells by proteomics	241
Patents benefit patients and patent reform would spur diagnostic and therapeutic development	240
Biotech news from around the world	239
Vector BioPharma: resurrecting adenoviral gene delivery	239
Multiplex-GAM detects complex chromatin interactions	233
Fluorescence microscopy resolution increases to near-atomic level	228
Eight diseases for gene therapy accelerator	226
People	224
Wearable devices	214
Editor’s pick: Tenpoint Therapeutics	214
Decoding the building blocks of cellular processes from single-cell transcriptomics data	208
Spanish tortillas go vegan	204
A base editor modifies bacterial genes in the guts of living mice	194
HIV drug 100% protective	193
Radical solutions	188
Big pharma craves slice of AI-based RNA drug discovery	185
Monitoring protein proximity with fluorogens	182
Nanopore sequencing	181
Detecting somatic mutations in single-cell data sets	179
Precise intraoperative brain tumor classification	177
Startups probe hidden viruses in the ‘dark genome’ to treat disease	170
An improved organoid model of the human cerebellum	165
Cancer Moonshot aims at liquid biopsies	164
Merck enlists trispecific killers	164
Single-cell multi-omics allows functional characterization of structural variants	161
AI’s plastic recycling	160
FDA okays first cardiac myosin inhibitor	159
Biotech news from around the world	158
Citizen chickpeas	156
Vision–language AI assistance in human pathology	154
Single-cell RNA-seq keeps cells alive	154
Biotech news from around the world	152
100,000 genomes — in Africa, for Africa	152
People	151
Has the PTAB made a difference in drug settlements and generic entry?	150
In vivo CRISPR agent cuts HAE attacks 95%	150
Supercharging T cell therapy with cancer mutations	149

Single-molecule peptide sequencing on semiconductor chips	145
Around the world in a month	144
Exploring tRNAs and their modifications and crosstalk using Nano-tRNAseq	144
Five questions with Weixin Tang	142
Lentiviral vector cleared of causing blood cancer	142
Editor’s pick: Switch Therapeutics	142
T cells	141
Cellular shuttles put mislocalized proteins in their right place	140
TRGT-ing the dark genome to accurately characterize tandem repeats at scale	139
An antigen discovery pipeline integrates multi-omics data and informs immunotherapy	139
Uncovering the global RNA virome	137
Government targets, end-of-year patenting rush and innovative performance in China	136
Industry moves on 3Rs	135
People	133
Organoids and organs on a chip	132
Personalized phosphoproteomics identifies functional signaling	129
Denmark invests in mucosal vaccines	128
Mining extinct proteomes for antimicrobial peptides	127
Capturing and modeling cellular niches from dissociated single-cell and spatial data	126
Generation of programmable splicing factors using RNA-binding proteins that activate exon inclusion	124
Sound healing and beyond	124
People	123
Wireless microelectronic fibers to discover gut and brain pathways	122
Genentech, Sangamo ink Alzheimer’s deal	122
CRISPR therapies march into clinic, but genotoxicity concerns linger	121
Chroma Medicine and Tune Therapeutics: Two companies take up epigenome editing	121
Identification of antimicrobial peptides from the human gut microbiome using deep learning	121
KARR-seq reveals cellular higher-order RNA structures and RNA–RNA interactions	119
Inferring cell–cell communication at single-cell resolution	119
Engineered CRISPR-Cas12a for higher-order combinatorial chromatin perturbations	119
Startup tackles oncogene-rich ecDNA	119
The lysosomal degraders	118
Bite-sized solutions	118
Real-time monitoring of tumor-homing bacteria and tumor cells in vivo using ultrasound	117
scChIX-seq infers dynamic relationships between histone modifications in single cells	116
Writing cellular history in protein chains	115
Drug licensing as evidence of evolution, diffusion and catch-up in East Asia	114
High-throughput retrieval of target sequences from complex clone libraries using CRISPRi	113
Rapid-response manufacturing of adenovirus-vectored vaccines	113
Mapping the patent landscape of medical machine learning	113
The rise of patient avatars in precision oncology	112
Enhanced cancer detection from cell-free DNA	109
Improving the predictive power of mouse models	109
Scalable in situ single-cell profiling by electrophoretic capture of mRNA using EEL FISH	109
Predicting disease variants using biodiversity and machine learning	109
Author Correction: Multiscale and integrative single-cell Hi-C analysis with Higashi	108
Fludarabine increases nuclease-free AAV- and CRISPR/Cas9-mediated homologous recombination in mice	108
Mapping mRNA modifications for functional studies	107
Integrating medicine, engineering and business to educate early-stage researchers in cardiovascular device development	105
What the COVID-19 pandemic revealed about intellectual property	105
Novel recombinases for large DNA insertions	105
The community of the DAO	103
Reflective multi-immersion microscope objectives inspired by the Schmidt telescope	103
The next giant step for microbes	102
First herpesvirus gene therapy	102
After obesity drugs’ success, companies rush to preserve skeletal muscle	102
gutSMASH predicts specialized primary metabolic pathways from the human gut microbiota	101
Improving combination drug trials using ‘definitive screening designs’	101
Survival of the fittest glia	100
The digital and analog worlds of protein engineering	100
The global patent landscape of artificial intelligence applications for cancer	98
Author Correction: Efficient C•G-to-G•C base editors developed using CRISPRi screens, target-library analysis, and machine learning	98
Do patent applications and Cooperative Research and Development Agreements between the National Cancer Institute and industry serve the public interest?	97
An optimized toolkit for prime editing	96
Generation of locus coeruleus norepinephrine neurons from human pluripotent stem cells	94
The prevalence of drug patent term extensions in the United States, 2000–2018	94
A new mass analyzer shakes up the proteomics field	93
Improving microbial phylogeny with citizen science within a mass-market video game	91
Contracts and intellectual property rights in translational R&D: furthering safeguards in the public interest	91
Engineered serum markers for non-invasive monitoring of gene expression in the brain	90
Evolutionary gambit to defeat drug resistance in cancer	90
Author Correction: Large-scale evaluation of the ability of RNA-binding proteins to activate exon inclusion	90
Long-term intravital subcellular imaging with confocal scanning light-field microscopy	89
Author Correction: Quantifying bias introduced by sample collection in relative and absolute microbiome measurements	88
A developmental route to hematopoietic stem cells	88
Accessible training for academic clinicians and researchers to address the need for academic entrepreneurs	87
Rapid generation of long, chemically modified pegRNAs for prime editing	87
Behind the graduate mental health crisis in science	87
NIS-Seq enables cell-type-agnostic optical perturbation screening	86
An update on Cuban biotech	85

CRISPR-StAR enables high-resolution genetic screening in complex in vivo models	85
Evaluating the analytical validity of circulating tumor DNA sequencing assays for precision oncology	84
Ovarian tumor cells gain competitive advantage by actively reducing the cellular fitness of microenvironment cells	84
More than just content: building community in the graduate classroom	82
NINDS launches network to develop treatments for ultra-rare neurological diseases	82
Fast and accurate metagenotyping of the human gut microbiome with GT-Pro	82
Dreaming ideal protein structures	82
A technical approach to global plant genome editing regulation	82
Author Correction: An injectable bone marrow–like scaffold enhances T cell immunity after hematopoietic stem cell transplantation	82
Multiplex de Bruijn graphs enable genome assembly from long, high-fidelity reads	81
Author Correction: Engineered pegRNAs improve prime editing efficiency	81
European patent protection for medical uses of known products and drug repurposing	80
Author Correction: LAMP-Seq enables sensitive, multiplexed COVID-19 diagnostics using molecular barcoding	80
Integrating innovation as a core objective in medical training	80
Publisher Correction: Integrative spatial analysis of cell morphologies and transcriptional states with MUSE	79
RNA delivery with a human virus-like particle	79
Identifying synergistic high-order 3D chromatin conformations from genome-scale nanopore concatemer sequencing	78
Framework for predicting alloreactivity in hematopoietic cell transplants	78
Virus-like elements	76
People	76
DNA writing technologies moving toward synthetic genomes	76
African coronavirus surveillance network provides early warning for world	75
Recoded gene circuits for multiplexed genetic code expansion	75
Publisher Correction: Drug hunters uncloak the non-coding ‘hidden’ genome	75
Machine learning in drug discovery	75
Overcoming unpredictability in biotech patents by tailored claiming aligned with genuine enablement	75
From air to your plate: tech startups making food from atmospheric CO2	75
Diabetes cell therapies take evasive action	74
COVID-19 long haulers	74
Drug hunters uncloak the non-coding ‘hidden’ genome	74
AI drug discovery booms in China	73
People	73
2Q21—a slowing of the pace	72
Biotech news from around the world	72
Nature Biotechnology’s academic spinouts 2020	71
Monkeypox response relies on three vaccine suppliers	71
Drug pipeline 1Q21—the old and the new	70
People	70
Drug pipeline 1Q23 — Everything everywhere all over the place	69
Long COVID immune phenotypes identified for blockade	69
Imbalanced single-cell data integration leads to loss of biological information	68
Drug pipeline 1Q22 — a raft of new modalities … and clinical blowups	68
Big mRNA players focus on flu vaccines	68
Waive COVID-19 IP rights, poor nations urge	68
Slivers of the spectrum	67
Artificial intelligence-guided design of lipid nanoparticles for pulmonary gene therapy	66
Inferring gene regulatory networks from single-cell multiome data using atlas-scale external data	66
A competitive precision CRISPR method to identify the fitness effects of transcription factor binding sites	66
High-throughput continuous evolution of compact Cas9 variants targeting single-nucleotide-pyrimidine PAMs	66
AI for drug discovery is booming, but who owns the patents?	66
Spatial tropism profiling of AAV vectors by ultrasensitive sequential FISH in tissue	66
RNA drugs lower lipoprotein(a) and genetically driven cholesterol	66
In vivo development of immune tissue in human intestinal organoids transplanted into humanized mice	65
Negative innovation: when patents are bad for patients	65
Dynamic, adaptive sampling during nanopore sequencing using Bayesian experimental design	65
Prediction of drug efficacy from transcriptional profiles with deep learning	65
Publisher Correction: Multistate and functional protein design using RoseTTAFold sequence space diffusion	64
Activation of recombinases at specific DNA loci by zinc-finger domain insertions	64
Generative models for protein structures and sequences	64
Unprecedented blood biomarker enables ALS drug approval	64
Estimation of tumor cell total mRNA expression in 15 cancer types predicts disease progression	64
Predicting transcriptional outcomes of novel multigene perturbations with GEARS	64
Massively parallel phenotyping of coding variants in cancer with Perturb-seq	63
Single-cell immunology of SARS-CoV-2 infection	63
Spatial mapping of the total transcriptome by in situ polyadenylation	63
Automated reconstruction of whole-embryo cell lineages by learning from sparse annotations	63
CRISPR-free, strand-selective mitochondrial DNA base editing using a nickase	62
Personalized medicine is having its day	62
Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome	62
Base editing marches on the clinic	61
A multiplex implantable microdevice assay identifies synergistic combinations of cancer immunotherapies and conventional drugs	61
Strand-selective base editing of human mitochondrial DNA using mitoBEs	61
Helping others enhances graduate student wellness and mental health	61
Induced proximity at the cell surface	60
Development of compact transcriptional effectors using high-throughput measurements in diverse contexts	60
Solid-phase capture and profiling of open chromatin by spatial ATAC	60
A knowledge graph to interpret clinical proteomics data	60
Engineered live bacteria suppress Pseudomonas aeruginosa infection in mouse lung and dissolve endotracheal-tube biofilms	60
Protein design: the experts speak	59
Supervised discovery of interpretable gene programs from single-cell data	59
Trans-nuclease activity of Cas9 activated by DNA or RNA target binding	59
Sparks of function by de novo protein design	59
Expanding access to CAR T cell therapies through local manufacturing	58
Putting CRISPR into African hands to future-proof crops	58
Mapping multimodal phenotypes to perturbations in cells and tissue with CRISPRmap	58
Chem-map profiles drug binding to chromatin in cells	57
How to grow (almost) anything: a hybrid distance learning model for global laboratory-based synthetic biology education	57
Transcriptome-wide profiling and quantification of N6-methyladenosine by enzyme-assisted adenosine deamination	57
A proteome-wide atlas of drug mechanism of action	56
A spatial genome aligner for resolving chromatin architectures from multiplexed DNA FISH	56
Chemical and topological design of multicapped mRNA and capped circular RNA to augment translation	56
Characterizing cellular heterogeneity in chromatin state with scCUT&Tag-pro	56
Droplet-based transcriptome profiling of individual synapses	56
Ebola as a case study for the patent landscape of medical countermeasures for emerging infectious diseases	55
A microneedle vaccine printer for thermostable COVID-19 mRNA vaccines	55