Nature Biotechnology

Article	Citations
Single-cell RNA-seq keeps cells alive	4335
Fungal architecture is a thing, and it’s eye-catching	2133
Synthetic biologics provide a path to target bHLH transcription factors	1177
AI’s plastic recycling	1006
People	927
Denmark invests in mucosal vaccines	751
Wireless microelectronic fibers to discover gut and brain pathways	714
Overcoming unpredictability in biotech patents by tailored claiming aligned with genuine enablement	708
Drug pipeline 1Q22 — a raft of new modalities … and clinical blowups	521
Framework for predicting alloreactivity in hematopoietic cell transplants	519
Bite-sized solutions	518
An optimized toolkit for prime editing	472
People	467
Design of optimized epigenetic regulators for durable gene silencing with application to PCSK9 in nonhuman primates	464
Behind the graduate mental health crisis in science	462
Automated reconstruction of whole-embryo cell lineages by learning from sparse annotations	423
Enhancing prime editing in hematopoietic stem and progenitor cells by modulating nucleotide metabolism	389
Reflective multi-immersion microscope objectives inspired by the Schmidt telescope	385
Orchestration of secondary metabolite production in Streptomyces species	381
Author Correction: LAMP-Seq enables sensitive, multiplexed COVID-19 diagnostics using molecular barcoding	375
Author Correction: Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew	365
Democratizing knowledge representation with BioCypher	352
An oncolytic virus–T cell chimera for cancer immunotherapy	334
High-efficiency transgene integration by homology-directed repair in human primary cells using DNA-PKcs inhibition	320
Synthetic biology’s uncertain regulatory future in the wake of Loper Bright Enterprises v. Raimondo	303

Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome	302
Establishing a commercial solution for extremely rare genetic diseases	284
Agentic AI and the rise of in silico team science in biomedical research	253
Multi-omics single-cell data integration and regulatory inference with graph-linked embedding	252
Variant to function mapping at single-cell resolution through network propagation	251
Safer non-viral DNA delivery using lipid nanoparticles loaded with endogenous anti-inflammatory lipids	249
Programmable promoter editing for precise control of transgene expression	241
Predicting transcriptional outcomes of novel multigene perturbations with GEARS	233
Engineering innate immune cells for cancer immunotherapy	231
Generative models for protein structures and sequences	229
Artificial intelligence-guided design of lipid nanoparticles for pulmonary gene therapy	228
The lysosomal degraders	227
Dual SORT LNPs for multi-organ base editing	226
Cellular shuttles put mislocalized proteins in their right place	225
Diffusion model expands RoseTTAFold’s power	223
TRGT-ing the dark genome to accurately characterize tandem repeats at scale	219
Biopharma dives into tumor-seeking radioactive drugs	219
Integrating medicine, engineering and business to educate early-stage researchers in cardiovascular device development	217
Fluorescence microscopy resolution increases to near-atomic level	209
Cancer Moonshot aims at liquid biopsies	207
A competitive precision CRISPR method to identify the fitness effects of transcription factor binding sites	203
Author Correction: Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion	201
Human periportal liver assembloids	199
Droplet-based transcriptome profiling of individual synapses	197
The world’s first CRISPR therapy is approved: who will receive it?	196
Chem-map profiles drug binding to chromatin in cells	193
Fresh from the biotech pipeline: FDA turmoil overshadows 2025 approvals	190
In vivo development of immune tissue in human intestinal organoids transplanted into humanized mice	190
Engineered serum markers for non-invasive monitoring of gene expression in the brain	188
AI predicts cancer relapse from slides	187
Single-cell nanobody-based profiles of multiple epigenetic modalities and chromatin velocity	187
Biotech news from around the world	185
3Q22 — more malaise in the markets	184
The need for need-finding in medical education	184
Biotech news from around the world	183
People	182
The promises and challenges of neoantigen cancer vaccines	182
HSV-1’s contribution as a vector for gene therapy	182
Live cell imaging of signaling networks using a conventional microscope	182
Slaughter-free meat hits the grocery shelf	181
Delix Therapeutics : psychedelics without the trip	176
Towards the non-invasive imaging of brain networks and functions at high resolution	174
Grand challenges in bioinformatics education and training	171
Prediction of peptide mass spectral libraries with machine learning	163
Sequence Display enables large-scale sequence–activity datasets for rapid protein evolution	161
Next-generation psychedelics: should new agents skip the trip?	158
Computationally designed enzymes show potent catalytic activity	157
David Baltimore (1938–2025)	157
Adrestia Therapeutics — Gene networks to the rescue	157
GLP-1s surprise failure in Alzheimer’s	155

Connecting research and clinical practice: training the next generation of translational scientists	152
Orna Therapeutics: circular logic	151
Artificial miRNA slows Huntington’s	150
Mapping the patent landscape of cancer diagnostics	147
Author Correction: The cost of drug patent expiration date errors	146
A language model beats alphafold2 on orphans	145
Hallucinating functional protein sequences	143
Escaping analysis paralysis in medical training: from FRAZZLE to CALM	142
Retraction Note: Rescue of the spinal muscular atrophy phenotype in a mouse model by early postnatal delivery of SMN	141
Engineered phage with antibacterial CRISPR–Cas selectively reduce E. coli burden in mice	140
Gains in early-onset dementia with progranulin open new paths for drug discovery	140
Considerations for the future of in vitro gametogenesis in fertility care	139
Protection of cell therapeutics from antibody-mediated killing by CD64 overexpression	137
Mesenchymal thymic niche cells enable regeneration of the adult thymus and T cell immunity	136
Decoding gene regulation with CRISPR perturbations	131
FDA approves first MASH drug	130
Lessons from biotech’s unscientific evolution	129
Precise DNA cleavage using CRISPR-SpRYgests	126
In vivo base editing reverses a neurodevelopmental disorder	126
Delivering 3 billion doses of Comirnaty in 2021	124
Improving metagenome binning by integrating intrinsic features and taxonomy	123
Building synthetic chromosomes one yeast at a time: insights from Sc2.0	123
Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes	122
2022 – toughing out the trough	121
A strategy to load, rethread and read protein sequences through a nanopore	121
Detection of mosaic and population-level structural variants with Sniffles2	121
Drug pipeline 4Q22 — sticking around	120
Forum: Boyden and Danzl	118
Biotech news from around the world	118
Lab-made antibody stops malaria	117
Are ecological processes that select beneficial traits in agricultural microbes nature’s intellectual property rights?	116
Genome-edited farm animals from haploid stem cells	114
Large-scale discovery of chromatin dysregulation induced by oncofusions and other protein-coding variants	114
Microbiome-friendly phages join the campaign for better antimicrobials	113
The devolution of biosimilars regulations	113
Platform solutions for commercial challenges to expanding patient access and making gene editing sustainable	113
Unpaired data as a first-order challenge in single-cell and spatial proteomics	112
Prediction of prime editing insertion efficiencies using sequence features and DNA repair determinants	112
Growth of biotech clusters over several decades through pioneering, variety and entrepreneurial science	110
Genome engineering with Cas9 and AAV repair templates generates frequent concatemeric insertions of viral vectors	109
Engineering pattern recognition receptors facilitates plant resistance breeding	108
Chronic nasal congestion gets new treatment	107
Consternation follows Marks’ FDA departure	107
Editor’s pick: Red Queen Therapeutics	107
Enzymatic synthesis of RNA oligonucleotides	107
Chemigenetic kinase biosensors illuminate cell signaling networks	106
Quantifying endosomal escape in vivo to guide lipid nanoparticle design	106
A circularly permuted CasRx platform for efficient, site-specific RNA editing	105
Cell type inference in cell-free nucleic acid liquid biopsy	105
Data sharing in the age of deep learning	103
Control of mammalian gene expression by modulation of polyA signal cleavage at 5′ UTR	102
Modular cytokine receptor-targeting chimeras for targeted degradation of cell surface and extracellular proteins	101
Epicardioid single-cell genomics uncovers principles of human epicardium biology in heart development and disease	100
Improving non-viral knock-in with modified single-stranded DNAs and small molecules	99
Synthetic microbial co-cultures for modular bioelectronic sensing in diverse environments	98
The global patent landscape of optogenetics: current advances, challenges and opportunities	98
Spatial host–microbiome sequencing reveals niches in the mouse gut	96
Rapid, scalable assessment of SARS-CoV-2 cellular immunity by whole-blood PCR	96
Tension-tuned receptors for synthetic mechanotransduction and intercellular force detection	96
RNA stability enhancers for durable base-modified mRNA therapeutics	95
Precision RNA base editing with engineered and endogenous effectors	95
Contamination source modeling with SCRuB improves cancer phenotype prediction from microbiome data	95
How will generative AI disrupt data science in drug discovery?	95
2024: research in review	94
A fully integrated wearable ultrasound system to monitor deep tissues in moving subjects	94
Base editors	94
An organoid model with all three pancreatic lineages resembles fetal pancreas	93
Stem cells	93
Ionis wins FDA nod for triglyceride-lowering rare disease drug	93
People	93
Relieving patent-eligibility barriers in biotech with a preparation or treatment method	92
A genome-wide view of disordered proteins	91
Reply to: Methodological concerns and lack of evidence for single-synapse RNA-seq	91
Tracking gene order provides a new perspective on intraspecific evolution in microbiotas	90
Thinking about a move from academia to industry? Look before you leap	90
Five questions with César de la Fuente	90
Cross-species snakebite antivenom	90
Children with rare genetic diseases get CRISPR Cures center	89
Author Correction: Precise, predictable multi-nucleotide deletions in rice and wheat using APOBEC–Cas9	89
Author Correction: Bioinstructive implantable scaffolds for rapid in vivo manufacture and release of CAR-T cells	89

Author Correction: Engineering circular RNA for enhanced protein production	89
Spotlight on cancer immunotherapies	88
Gene drives tested against real-world malaria diversity	88
Next-generation patient models for colorectal cancer research	88
AI-enhanced protein design makes proteins that have never existed	88
Septerna: making another run on GPCRs	85
Genotoxins from gut bacteria	84
TCR cell therapies vanquish solid tumors — finally	83
The strength and importance of government-funded patents for approved drugs	83
Generation of accurate, expandable phylogenomic trees with uDance	83
Sensitive detection of gene transfer in a microbial community	83
Post-translational modifications reshape the antigenic landscape of the MHC I immunopeptidome in tumors	82
Human respiratory airway progenitors derived from pluripotent cells generate alveolar epithelial cells and model pulmonary fibrosis	82
Facile repurposing of peptide–MHC-restricted antibodies for cancer immunotherapy	82
Multifunctional microelectronic fibers enable wireless modulation of gut and brain neural circuits	79
Tuning plant phenotypes by precise, graded downregulation of gene expression	79
A community effort to optimize sequence-based deep learning models of gene regulation	79
Growth-coupled microbial biosynthesis of the animal pigment xanthommatin	78
Mapping medically relevant RNA isoform diversity in the aged human frontal cortex with deep long-read RNA-seq	78
High-precision cytosine base editors by evolving nucleic-acid-recognition hotspots in deaminase	78
Senolytics under scrutiny in the quest to slow aging	78
How will AI affect patent disclosures?	77
Accurate somatic small variant discovery for multiple sequencing technologies with DeepSomatic	77
High-throughput discovery of MHC class I- and II-restricted T cell epitopes using synthetic cellular circuits	77
Discovery of drug–omics associations in type 2 diabetes with generative deep-learning models	76
The GA4GH Phenopacket schema defines a computable representation of clinical data	76
Hypoimmune induced pluripotent stem cells survive long term in fully immunocompetent, allogeneic rhesus macaques	76
Systema: a framework for evaluating genetic perturbation response prediction beyond systematic variation	75
Precise integration of large DNA sequences in plant genomes using PrimeRoot editors	75
Spatial genomics of AAV vectors reveals mechanism of transcriptional crosstalk that enables targeted delivery of large genetic cargo	75
Efficient prime editing in mouse brain, liver and heart with dual AAVs	74
3Q23 — VCs feed ‘older children’ as IPO markets stagnate	73
Measuring the impact of chromatin context on transcription factor binding affinities	73
Intestinal mucosal barrier repair and immune regulation with an AI-developed gut-restricted PHD inhibitor	73
People	73
2H23 biotech job picture	72
Microfluidics	71
Base editor restores CD3δ-dependent SCID mutation	71
Microbiomics	71
Discovering the crucial function of long noncoding RNAs	70
Bridge RNAs direct programmable DNA rearrangements	70
Big pharma seduced by transcription factors again. What has changed?	69
Startup grows egg proteins in potato fields	69
Embryonic stem cells across bird species	69
Using artificial intelligence to develop gene therapy for the lungs	69
Combining reference genomes into a pangenome graph improves accuracy and reduces bias	69
Fighting fibrosis	68
AAV-delivered UGA suppressor tRNA for disease-agnostic in vivo gene therapy	67
Fresh from the biotech pipeline: FDA approvals settle in 2024, but what next?	67
A shifting market environment and strategies fueling innovation in prostate cancer treatment	66
Prime editing	66
Eli Lilly in $650M pact to boost muscle	66
Starving tumors with fat	66
Make science disruptive again	66
Gabriele et al. reply	66
Wearable ultrasound for continuous deep-tissue monitoring	64
Linking CRISPR–Cas9 double-strand break profiles to gene editing precision with BreakTag	63
Resurrecting a miniature Cas9 ancestor for genome and epigenome editing	63
RNA interference in the era of nucleic acid therapeutics	63
Biotech patenting 2021	63
De novo detection of somatic mutations in high-throughput single-cell profiling data sets	62
Capturing the diversity of protein modifications on presented tumor antigens	62
The instant spray-on dress	62
Redefining antibody patent protection using paratope mapping and CDR-scanning	61
Culturing microbiome therapeutics with big data	61
Scalable single-cell RNA sequencing from full transcripts with Smart-seq3xpress	60
Chikungunya vaccine approved	60
Uniform volumetric single-cell processing for organ-scale molecular phenotyping	60
Precise, minimally evolved adenine base editors generated through mutation reversion analysis	60
Deep tissue multi-photon imaging using adaptive optics with direct focus sensing and shaping	59
Single-molecule live-cell RNA imaging with CRISPR–Csm	59
Generation of a live attenuated influenza A vaccine by proteolysis targeting	58
Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity	56
Lipid nanoparticle-mediated mRNA delivery to CD34+ cells in rhesus monkeys	56
The challenges and promise of sweat sensing	55
Predicting small molecule–RNA interactions without RNA tertiary structures	55
A germ-free humanized mouse model shows the contribution of resident microbiota to human-specific pathogen infection	55
A resource of RNA-binding protein motifs across eukaryotes reveals evolutionary dynamics and gene-regulatory function	55
Ultra-fast label-free quantification and comprehensive proteome coverage with narrow-window data-independent acquisition	55
Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases	54
Second-quarter biotech job picture	54
The reverse transcriptase domain of prime editors contributes to DNA repair in mammalian cells	54
Challenges for developing broad-based mucosal vaccines for respiratory viruses	54
Biotech news from around the world	54
High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails	54
Public biotech in 2021 — the numbers	54
Visualizing protein nanostructures in intact brain	54
A retrotransposon for site-specific gene transfer	53
Biotech news from around the world	53
Wasp gut microbes yield beer with extra tang	53
Biomedical imaging	53
The long game of tenure	53
Forum: Locke and Sykes	53
Radiopharmaceuticals	52
MicroRNAs	52