Metabolic Engineering

Article	Citations
Genome-wide CRISPR screening identifies genes in recombinant human embryonic kidney 293 cells for increased ammonia resistance	94
Introduction of acetyl-phosphate bypass and increased culture temperatures enhanced growth-coupled poly-hydroxybutyrate production in the marine cyanobacterium Synechococcus sp. PCC7002	82
Alternative routes for production of the drug candidate d-chiro-inositol with Corynebacterium glutamicum using endogenous or promiscuous plant enzymes	81
Engineering Halomonas bluephagenesis for high-efficiency biosynthesis of pyruvate	79
Engineering sub-organelles of a diploid Saccharomyces cerevisiae to enhance the production of 7-dehydrocholesterol	69
Pan-reactome analysis of Streptomyces strains reveals association and disconnection between primary and secondary metabolism	67
Efficient production of (S)-limonene and geraniol in Saccharomyces cerevisiae through the utilization of an Erg20 mutant with enhanced GPP accumulation capability	66
Development of a thermophilic l-arabinose-inducible system in Acetivibrio thermocellus (Clostridium thermocellum)	66
Engineering carbon source division of labor for efficient α-carotene production in Corynebacterium glutamicum	65
Butyrate as a growth factor of Clostridium acetobutylicum	64
Elucidation and engineering of Sphingolipid biosynthesis pathway in Yarrowia lipolytica for enhanced production of human-type sphingoid bases and glucosylceramides	61
Engineering Yarrowia lipolytica for sustainable ricinoleic acid production: A pathway to free fatty acid synthesis	60
Engineering a biosensor based high-throughput screening platform for high-yield caffeic acid production in Escherichia coli	60
Metabolic engineering of Escherichia coli for the production of two-carbon primary amines	58
Improved arginine production in Escherichia coli by harnessing the intracellular citrulline	58
Heterologous biosynthesis of betanin triggers metabolic reprogramming in tobacco	58
Engineering Halomonas bluephagenesis for pilot production of terpolymers containing 3-hydroxybutyrate, 4-hydroxybutyrate and 3-hydroxyvalerate from glucose	58
13C-Metabolic flux analysis of 3T3-L1 adipocytes illuminates its core metabolism under hypoxia	57
Pathway elucidation and microbial synthesis of proaporphine and bis-benzylisoquinoline alkaloids from sacred lotus (Nelumbo nucifera)	56
Inferring secretory and metabolic pathway activity from omic data with secCellFie	55
Engineering an SspB-mediated degron for novel controllable protein degradation	53
Biosynthesis of 12-aminododecanoic acid from biomass sugars	53
Identification of a specific exporter that enables high production of aconitic acid in Aspergillus pseudoterreus	51
De novo 2′-fucosyllactose biosynthesis using glucose as the sole carbon source by multiple engineered Bacillus subtilis	51
Coordinating caffeic acid and salvianic acid A pathways for efficient production of rosmarinic acid in Escherichia coli	51

Quantifying supply and demand in the pea aphid-Buchnera symbiosis reveals the metabolic Achilles’ heels of this interaction	49
Optimized production of concanamycins using a rational metabolic engineering strategy	48
Development of a Streptomyces-based system for facile thioholgamide library generation and analysis	47
Versatile xylose and arabinose genetic switches development for yeasts	45
Machine-learning guided elucidation of contribution of individual steps in the mevalonate pathway and construction of a yeast platform strain for terpenoid production	45
Disruption of acyl-acyl carrier protein (acyl-ACP) synthetase in cyanobacteria impairs lipid remodeling as revealed by acyl-ACP measurements	44
Silk fibroin production in Escherichia coli is limited by a positive feedback loop between metabolic burden and toxicity stress	43
Systematic study of genomic loci in Escherichia coli B and K12 for genomic integration: application in plasmid-free astaxanthin production	43
Consistent biosynthesis of D-glycerate from variable mixed substrates	43
Engineering diverse fatty acid compositions of phospholipids in Escherichia coli	43
Rethinking 13C-metabolic flux analysis – The Bayesian way of flux inference	43
Consolidated bioprocessing for bioethanol production by metabolically engineered cellulolytic fungus Myceliophthora thermophila	42
COSMIC-dFBA: A novel multi-scale hybrid framework for bioprocess modeling	42
Auto-inducible synthetic pathway in E. coli enhanced sustainable indigo production from glucose	42
Deep learning for NAD/NADP cofactor prediction and engineering using transformer attention analysis in enzymes	42
Metabolic engineering of Escherichia coli for N-methylserotonin biosynthesis	42
Unlocking lager's flavour palette by metabolic engineering of Saccharomyces pastorianus for enhanced ethyl ester production	42
Reconstruction of arginine deiminase pathway sustains a higher-energy state in mammalian cells	41
Metabolic engineering of microbial pathways for 5-aminolevulinic acid biosynthesis: Recent advances and biotechnological applications	41
Metabolic engineering of commensal bacteria for gut butyrate delivery and dissection of host-microbe interaction	40
Time-resolved, deuterium-based fluxomics uncovers the hierarchy and dynamics of sugar processing by Pseudomonas putida	40
A novel step towards the heterologous biosynthesis of paclitaxel: Characterization of T1βOH taxane hydroxylase	40
Insights into the methanol utilization capacity of Y. lipolytica and improvements through metabolic engineering	38
Functional genomic screening in Komagataella phaffii enabled by high-activity CRISPR-Cas9 library	38
A reactive species reactions module for integration into genome-scale metabolic models for improved insights: Application to cancer	38
Engineering Rhodosporidium toruloides for production of 3-hydroxypropionic acid from lignocellulosic hydrolysate	37
De novo production of 1,3-olein-2-palmitin (OPO) and 1-olein-2-palmitin-3-linolein (OPL) by multiplexed reconstruction of lipid metabolism in yeasts	37
A Pseudomonas taiwanensis malonyl-CoA platform strain for polyketide synthesis	37
Engineering and evolution of the complete Reductive Glycine Pathway in Saccharomyces cerevisiae for formate and CO2 assimilation	37
Boosting titers of engineered triketide and tetraketide synthases to record levels through T7 promoter tuning	37
A database of over 15,000 strain design publications reveals a conserved set of metabolic engineering targets across microbial hosts and products	36
Corrigendum to “Tandem chemical deconstruction and biological upcycling of poly(ethylene terephthalate) to β-ketoadipic acid by Pseudomonas putida KT2440” (Metab. Eng. 67 (2021) 250–261)	35
Editorial Board	35
Editorial Board	35
Selective microbial production of lacto-N-fucopentaose I in Escherichia coli using engineered α-1,2-fucosyltransferases	34
Metabolic engineering of artificially modified transcription factor SmMYB36-VP16 for high-level production of tanshinones and phenolic acids	34
Repurposing plant hormone receptors as chemically-inducible genetic switches for dynamic regulation in yeast	34
Hybrid biological-chemical strategy for converting polyethylene into a recyclable plastic monomer using engineered Corynebacterium glutamicum	34
Co-cultivation of Saccharomyces cerevisiae strains combines advantages of different metabolic engineering strategies for improved ethanol yield	33
Verazine biosynthesis from simple sugars in engineered Saccharomyces cerevisiae	33
Rational engineering to enhance C8-fatty acid biosynthesis in Picosynechococcus sp. PCC 7002	33
Flux optimization using multiple promoters in Halomonas bluephagenesis as a model chassis of the next generation industrial biotechnology	33
Systems metabolic engineering upgrades Corynebacterium glutamicum for selective high-level production of the chiral drug precursor and cell-protective extremolyte L-pipecolic acid	33
Corrigendum to “De novo biosynthesis of the polymethoxylated flavones eupatilin and jaceosidin in Saccharomyces cerevisiae” [Metabol. Eng. 97 (2026), 102502]	33
Reusable and modular combinatorial libraries for iterative metabolic engineering of Saccharomyces cerevisiae	32
Systems metabolic engineering of Corynebacterium glutamicum eliminates all by-products for selective and high-yield production of the platform chemical 5-aminovalerate	32
Targeting cofactors regeneration in methylation and hydroxylation for high level production of Ferulic acid	32
Self-controlled in silico gene knockdown strategies to enhance the sustainable production of heterologous terpenoid by Saccharomyces cerevisiae	32
Electrical-biological hybrid system for carbon efficient isobutanol production	32
Ectoine hyperproduction by engineered Halomonas bluephagenesis	31

Evaluation of strategies to narrow the product chain-length distribution of microbially synthesized free fatty acids	31
Stress-driven dynamic regulation of multiple genes to reduce accumulation of toxic aldehydes	31
Engineering of insect juvenile hormone III biosynthesis in the plant Nicotiana benthamiana	31
High-yield production of FK228 and new derivatives in a Burkholderia chassis	31
Developing a Bacillus licheniformis platform for de novo production of γ-aminobutyric acid and other glutamate-derived chemicals	30
Editorial Board	30
Systems metabolic engineering of Klebsiella pneumoniae for high-level 1,3-propanediol production	30
Efficient production of natural sunscreens shinorine, porphyra-334, and mycosporine-2-glycine in Saccharomyces cerevisiae	30
Bioprocess optimisation via joint machine learning and metabolic modelling	30
Establishing biosynthetic pathways for the production of nicotinic acid and trigonelline in Escherichia coli	29
Biomass accumulation in chondrocyte metabolic modelling: Incorporating extracellular matrix proxies to predict tissue engineering outcomes	29
Orthogonal quorum sensing circuits enable dynamic regulation in Escherichia coli	29
Fluctuating pH for efficient photomixotrophic succinate production	29
CRISPRi-microfluidics screening enables genome-scale target identification for high-titer protein production and secretion	29
Generation of a Vibrio-based platform for efficient conversion of raffinose through Adaptive Laboratory Evolution on a solid medium	29
Sustainable production of genistin from glycerol by constructing and optimizing Escherichia coli	29
Sustainable production through spatial niche partitioning in engineered light-driven microbial community	29
Omics analyses decoding mechanisms underlying the self-flocculating phenotype of yeast cells and stress tolerance for robust production	29
Adaptive evolution of engineered Saccharomyces cerevisiae in favored and unusual chemical environments	28
High-level and -yield production of L-leucine in engineered Escherichia coli by multistep metabolic engineering	27
Evolution-assisted engineering of E. coli enables growth on formic acid at ambient CO2 via the Serine Threonine Cycle	27
Metabolic engineering of Corynebacterium glutamicum for increased cis, cis-muconate production from plant-derived p-hydroxycinnamates via deregulated pathway flux and increased CoA intermediate availa	27
Minimized antibiotic-free plasmid vector for gene therapy utilizing a new toxin-antitoxin system	27
Metabolic engineering of Acinetobacter baylyi ADP1 for efficient utilization of pentose sugars and production of glutamic acid	27
KETCHUP: Parameterizing of large-scale kinetic models using multiple datasets with different reference states	27
Engineering 5-hydroxymethylfurfural (HMF) oxidation in Pseudomonas boosts tolerance and accelerates 2,5-furandicarboxylic acid (FDCA) production	27
Overexpression of peroxisome proliferator-activated receptor γ co-activator-1⍺ (PGC-1⍺) in Chinese hamster ovary cells increases oxidative metabolism and IgG productivity	27
Editorial Board	26
Rational search of genetic design space for a heterologous terpene metabolic pathway in Streptomyces	26
Unraveling productivity-enhancing genes in Chinese hamster ovary cells via CRISPR activation screening using recombinase-mediated cassette exchange system	26
Establishing heterologous betaxanthin pigment biosynthesis in cyanobacteria	26
Genome mining of tailoring enzymes from biosynthetic gene clusters for synthetic biology: A case study with fungal methyltransferases	26
A tunable metabolic valve for precise growth control and increased product formation in Pseudomonas putida	26
Metabolic engineering of Escherichia coli for high production of 1,5-pentanediol via a cadaverine-derived pathway	26
Engineering of Escherichia coli cytoplasm and periplasm for efficient synthesis of salvianic acid A	26
Single cell oil enriched in ricinoleic acid biosynthesis from glucose via metabolically rewired oleaginous yeast	25
Enzyme engineering for optimizing biosynthesis of 2,4-dihydroxybutyric acid via the synthetic threose-dependent glycolaldehyde assimilation (STEGA) pathway	25
A dynamic kinetic model captures cell-free metabolism for improved butanol production	24
Efficient production of retinol in Yarrowia lipolytica by increasing stability using antioxidant and detergent extraction	24
Development of an efficient yeast platform for cannabigerolic acid biosynthesis	24
Demonstration and technoeconomic analysis of dodecanol production from acetate using metabolically engineered Escherichia coli	24
A tRNA Modification-based strategy for Identifying amiNo acid Overproducers (AMINO)	24
Coordinated reprogramming of ATP metabolism strongly enhances adipic acid production in Escherichia coli	24
Laboratory evolution reveals general and specific tolerance mechanisms for commodity chemicals	24
Laboratory evolution of Synechocystis sp. PCC 6803 for phenylpropanoid production	24
Proteome trade-off between primary and secondary metabolism shapes acid stress induced bacterial exopolysaccharide production	24
Genome-scale and pathway engineering for the sustainable aviation fuel precursor isoprenol production in Pseudomonas putida	24
Engineered non-canonical reductive TCA pathway drives high-yield succinic acid biosynthesis in Yarrowia lipolytica	23
Thermodynamic limitations of PHB production from formate and fructose in Cupriavidus necator	23
Corrigendum to “Microbial production of propionic acid through a novel β-alanine route” [Metabol. Eng. (2026) 219–231 93]	23
AI-based automated construction of high-precision Geobacillus thermoglucosidasius enzyme constraint model	23
Engineering Escherichia coli for the production of saturated archaeal lipids	23
dCas9-mediated dysregulation of gene expression in human induced pluripotent stem cells during primitive streak differentiation	23
Engineering xylose catabolism in the yeast Komagataella phaffii	22
Multi-dimensional experimental and computational exploration of metabolism pinpoints complex probiotic interactions	22
Enabling anaerobic growth of Escherichia coli on glycerol in defined minimal medium using acetate as redox sink	22
Encapsulation of select violacein pathway enzymes in the 1,2-propanediol utilization bacterial microcompartment to divert pathway flux	22
PET-FBA: A lightweight enzyme allocation and thermodynamics-constrained flux analysis approach to explore Escherichia coli metabolic adaptation to intracellular acidification	22
Ribo-seq guided design of enhanced protein secretion in Komagataella phaffii	22
Modulating fatty acid metabolism and composition of CHO cells by feeding high levels of fatty acids complexed using methyl-β-cyclodextrin	22
Expression of the extracellular electron transfer pathway of Acidithiobacillus ferrooxidans enabling Fe(III)-citrate reduction and anodic respiration in Escherichia coli	22
Engineering metabolic flux for the microbial synthesis of aromatic compounds	22
Cyanobacterial phycobilisomes as a platform for the stable production of heterologous enzymes and other proteins	22
Engineered plants for the production of the antioxidants arbutin and gallate	21
Big data and machine learning: Metabolic engineering special issue	21
Integrated rational and evolutionary engineering of genome-reduced Pseudomonas putida strains promotes synthetic formate assimilation	21
Engineering ethanologenicity into the extremely thermophilic bacterium Anaerocellum (f. Caldicellulosiriuptor) bescii	21
Advances in metabolic engineering of Vibrio natriegens as an unconventional host for biotechnology	21
Metabolic engineering improves transduction efficiency and downstream vector isolation by altering the lipid composition of extracellular vesicle-enclosed AAV	20
Corrigendum to “Metabolic and enzyme rewiring enables high-production of vanillin in unconventional yeast” [Metabol. Eng. 93 (2026) 158–167]	20
Editorial Board	20
Metabolic engineering of human gut microbiome: Recent developments and future perspectives	20
Accurate prediction of in vivo protein abundances by coupling constraint-based modelling and machine learning	20
Metabolic engineering of Corynebacterium glutamicum for vitamin B12-independent production of 3-hydroxypropionic acid	20
Editorial Board	20
Population genomics-guided engineering of phenazine biosynthesis in Pseudomonas chlororaphis	19
Metabolic impact of heterologous protein production in Pseudomonas putida: Insights into carbon and energy flux control	19
A nutrition algorithm to optimize feed and medium composition using genome-scale metabolic models	19
Multi-omics view of recombinant Yarrowia lipolytica: Enhanced ketogenic amino acid catabolism increases polyketide-synthase-driven docosahexaenoic production to high selectivity at the gram scale	19
Establishing a coumarin production platform by protein and metabolic engineering	19

Enhancing oil feedstock utilization for high-yield low-carbon polyhydroxyalkanoates industrial bioproduction	19
Engineering nucleotide sugar synthesis pathways for independent and simultaneous modulation of N-glycan galactosylation and fucosylation in CHO cells	19
Engineering Escherichia coli for efficient glutathione production	19
Combinatorial iterative method for metabolic engineering of Yarrowia lipolytica: Application for betanin biosynthesis	19
A metabolizable inducer–based carbon-source autoinduction (MICA) system enables low-cost, high-level protein expression and metabolic control in Bacillus subtilis	18
Production of species-specific anthocyanins through an inducible system in plant hairy roots	18
Evaluating proteome allocation of Saccharomyces cerevisiae phenotypes with resource balance analysis	18
High glutamate demand enables simultaneous consumption of glycerol and citrate despite carbon catabolite repression in engineered Bacillus subtilis strains	18
High-yield production of coenzyme F420 in Escherichia coli by fluorescence-based screening of multi-dimensional gene expression space	18
Engineering Komagataella phaffii to produce lycopene sustainably from glucose or methanol	18
Metabolic engineering of Escherichia coli for uridine-5′-diphosphate N-acetylglucosamine production through salvage pathway	18
One-pot selective biosynthesis of Tyrian purple in Escherichia coli	18
Editorial Board	18
Metabolic reprogramming and biosensor-assisted mutagenesis screening for high-level production of L-arginine in Escherichia coli	18
A contribution of metabolic engineering to addressing medical problems: Metabolic flux analysis	18
Dynamic regulation combined with systematic metabolic engineering for high-level palmitoleic acid accumulation in oleaginous yeast	17
Deciphering molecular drivers of lactate metabolic shift in mammalian cell cultures	17
Beyond CEN.PK - parallel engineering of selected S. cerevisiae strains reveals that superior chassis strains require different engineering approaches for limonene production	17
Strategic engineering for overproduction of oviedomycin, a Type II polyketide, in Escherichia coli	17
Efforts to install a heterologous Wood-Ljungdahl pathway in Clostridium acetobutylicum enable the identification of the native tetrahydrofolate (THF) cycle and result in early induction of solvents	17
Lipid accumulation in nitrogen and phosphorus-limited yeast is caused by less growth-related dilution	17
Bioconversion of CO2 into valuable bioproducts via synthetic modular co-culture of engineered Chlamydomonas reinhardtii and Escherichia coli	17
Edible fungus Fusarium venenatum: advances, challenges, and engineering strategies for future food production	16
Microbial production of creatine using growth-coupled selection systems	16
Development of Corynebacterium glutamicum as a monoterpene production platform	16
Multi-omics driven genome-scale metabolic modeling improves viral vector yield in HEK293	16
Optogenetic control of Cdc48 for dynamic metabolic engineering in yeast	16
Establishing an Autonomous Cascaded Artificial Dynamic (AutoCAD) regulation system for improved pathway performance	16
Adaptive laboratory evolution and genetic engineering improved terephthalate utilization in Pseudomonas putida KT2440	16
Metabolic engineering of Escherichia coli for the high-level production of putrescine	16
De novo biosynthesis of 3-hydroxy-3-methylbutyrate as anti-catabolic supplement by metabolically engineered Escherichia coli	16
Evolution-guided tolerance engineering of Pseudomonas putida KT2440 for production of the aviation fuel precursor isoprenol	16
Improved biosynthesis of C4 derivatives by engineered thiolase	16
Improving the soluble expression of difficult-to-express proteins in prokaryotic expression system via protein engineering and synthetic biology strategies	16
Cross-regulation between proteome reallocation and metabolic flux redistribution governs bacterial growth transition kinetics	16
Engineering oleaginous yeast Rhodotorula toruloides for production of alkanes and alkenes	16
Construction of a synthetic metabolic pathway for biosynthesis of threonine from ethylene glycol	16
Engineering yeast for high-level production of diterpenoid sclareol	16
A multilayered biocontainment system for laboratory and probiotic yeast	16
Designing glucose utilization "highway" for recombinant biosynthesis	16
Editorial Board	16
Tailoring Escherichia coli for high-yield production of O-acetyl-L-homoserine through multi-node metabolic regulation	16
Genome-scale models of metabolism and expression predict the metabolic burden of recombinant protein expression	16
Metabolic division engineering of Escherichia coli consortia for de novo biosynthesis of flavonoids and flavonoid glycosides	16
Rational design of a bacterial import system for new-to-nature molecules	15
Metabolic engineering of Corynebacterium glutamicum for the production of the low-caloric natural sweetener D-allulose via phosphorylated intermediates	15
Development of a salt-enhanced promoter strategy for activating silent biosynthetic gene clusters from streptomycetes	15
Engineering the cellulolytic bacterium, Clostridium thermocellum, to co-utilize hemicellulose	15
Improved terephthalic acid production from p-xylene using metabolically engineered Pseudomonas putida	15
Switching the yeast metabolism via manipulation of sugar phosphorylation	15
Precise biosynthesis of β-1,2-glucan from cellulosic materials by in vitro metabolic engineering	15
Directed evolution of biomass intensive CHO cells by adaptation to sub-physiological temperature	15
Efficient production of an antitumor precursor actinocin and other medicinal molecules from kynurenine pathway in Escherichia coli	15
Redefining HexR regulatory landscape in Pseudomonas putida KT2440 through integrative systems biology	15
Metabolic engineering in Hot Acid: Strategies enabling chemolithotrophy in thermoacidophilic archaea	15
A genome-scale dynamic constraint-based modelling (gDCBM) framework predicts growth dynamics, medium composition and intracellular flux distributions in CHO clonal variations	15
Enhancing the production of isopropanol with reduced CO2 emission via protein and metabolic engineering using Corynebacterium glutamicum	15
Initiation of fatty acid biosynthesis in Pseudomonas putida KT2440	15
Metabolic engineering of Halomonas bluephagenesis for production of five carbon molecular chemicals derived from L-lysine	14
Rank-ordering of known enzymes as starting points for re-engineering novel substrate activity using a convolutional neural network	14
A self-regulated network for dynamically balancing multiple precursors in complex biosynthetic pathways	14
Systematic rewiring of Bacillus subtilis for efficient de novo biosynthesis of the neuroprotectant cytidine-5′-diphosphocholine	14
Engineering low-salt growth Halomonas Bluephagenesis for cost-effective bioproduction combined with adaptive evolution	14
Enzyme activity engineering based on sequence co-evolution analysis	14
Modularized Engineering of Shewanella oneidensis MR-1 for Efficient and Directional Synthesis of 5-Aminolevulinic Acid	14
Evolution and engineering of pathways for aromatic O-demethylation in Pseudomonas putida KT2440	14
Synergistic Co-utilization of the prenol and methylerythritol phosphate pathways for maximizing cis-abienol biosynthesis in Escherichia coli	14
Engineering robust Saccharomyces cerevisiae for high-yield sabinene production from lignocellulosic hydrolysate	14
Deciphering the network of cholesterol biosynthesis in Paris polyphylla laid a base for efficient diosgenin production in plant chassis	14
Exploring the versatility of fatty acid biosynthesis in Escherichia coli: Production of random methyl branched fatty acids	14
BGC heteroexpression strategy for production of novel microbial secondary metabolites	13
The halo of future bio-industry based on engineering Halomonas	13
Reconstructing curcumin biosynthesis in yeast reveals the implication of caffeoyl-shikimate esterase in phenylpropanoid metabolic flux	13
Mechanism and engineering of endoplasmic reticulum-localized membrane protein folding in Saccharomyces cerevisiae	13
Design and application of a kinetic model of lipid metabolism in Saccharomyces cerevisiae	13
Production of cholesterol-like molecules impacts Escherichia coli robustness, production capacity, and vesicle trafficking	13
Plastidial engineering with coupled farnesyl diphosphate pool reconstitution and enhancement for sesquiterpene biosynthesis in tomato fruit	13
Riboswitch-guided chalcone synthase engineering and metabolic flux optimization for enhanced production of flavonoids	13
Evaluation of enzyme-constrained genome-scale model through metabolic engineering of anaerobic co-production of 2,3-butanediol and glycerol by Saccharomyces cerevisiae	13
Engineering phenylpyruvate decarboxylase for controlled biosynthesis of aromatic amino acid derivatives	13
Multidimensional engineering of Saccharomyces cerevisiae for the efficient production of heme by exploring the cytotoxicity and tolerance of heme	13
Pseudomonas vancouverensis as a platform for one-carbon (C1) assimilation and polyhydroxyalkanoate accumulation	13
Stochastic simulation algorithm for isotope-based dynamic flux analysis	13
Metabolic engineering of the acid-tolerant yeast Pichia kudriavzevii for efficient L-malic acid production at low pH	12
Development of a nonauxotrophic L-homoserine hyperproducer in Escherichia coli by systems metabolic engineering	12
Engineering the biological conversion of formate into crotonate in Cupriavidus necator	12
Systems metabolic engineering upgrades Corynebacterium glutamicum to high-efficiency cis, cis-muconic acid production from lignin-based aromatics	12
Plasmid-based electroporation for efficient genetic engineering in immortalized T lymphocytes	12
A long-term growth stable Halomonas sp. deleted with multiple transposases guided by its metabolic network model Halo-ecGEM	12
Serial adaptive laboratory evolution enhances mixed carbon metabolic capacity of Escherichia coli	12
Expression in CHO cells of a bacterial biosynthetic pathway producing a small non-ribosomal peptide aldehyde prevents proteolysis of recombinant proteins	12
Unleashing the innate ability of Escherichia coli to produce D-Allose	12
The 6-phosphofructokinase reaction in Acetivibrio thermocellus is both ATP- and pyrophosphate-dependent	12
De novo biosynthesis of butyl butyrate in engineered Clostridium tyrobutyricum	12
De novo biosynthesis of D-panthenol in engineered E. coli with rationally designed L-homoserine decarboxylase	12