Metabolic Engineering

Papers
(The median citation count of Metabolic Engineering is 9. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-04-01 to 2025-04-01.)
ArticleCitations
Introduction of acetyl-phosphate bypass and increased culture temperatures enhanced growth-coupled poly-hydroxybutyrate production in the marine cyanobacterium Synechococcus sp. PCC7002200
Exploring and increased acetate biosynthesis in Synechocystis PCC 6803 through insertion of a heterologous phosphoketolase and overexpressing phosphotransacetylase118
Scalable, robust, high-throughput expression & purification of nanobodies enabled by 2-stage dynamic control101
Engineering Halomonas bluephagenesis via small regulatory RNAs99
Reconstructing a recycling and nonauxotroph biosynthetic pathway in Escherichia coli toward highly efficient production of L-citrulline94
Engineering improved ethylene production: Leveraging systems biology and adaptive laboratory evolution87
A synthetic C2 auxotroph of Pseudomonas putida for evolutionary engineering of alternative sugar catabolic routes81
Assessing the impact of substrate-level enzyme regulations limiting ethanol titer in Clostridium thermocellum using a core kinetic model75
Directed evolution of biomass intensive CHO cells by adaptation to sub-physiological temperature70
Efficient production of an antitumor precursor actinocin and other medicinal molecules from kynurenine pathway in Escherichia coli63
Use of acetate as substrate for sustainable production of homoserine and threonine by Escherichia coli W3110: A modular metabolic engineering approach62
Large-scale pathway reconstruction and colorimetric screening accelerate cellular metabolism engineering62
A coculture-coproduction system designed for enhanced carbon conservation through inter-strain CO2 recycling59
Editorial Board59
Fluctuating pH for efficient photomixotrophic succinate production57
Minimized antibiotic-free plasmid vector for gene therapy utilizing a new toxin-antitoxin system57
Editorial Board55
Biosynthesis of β-lactam nuclei in yeast54
G6P-capturing molecules in the periplasm of Escherichia coli accelerate the shikimate pathway50
Genetic and biocatalytic basis of formate dependent growth of Escherichia coli strains evolved in continuous culture49
Editorial Board49
Editorial Board49
Sustainable production of genistin from glycerol by constructing and optimizing Escherichia coli48
Initiation of fatty acid biosynthesis in Pseudomonas putida KT244048
Introduction of NADH-dependent nitrate assimilation in Synechococcus sp. PCC 7002 improves photosynthetic production of 2-methyl-1-butanol and isobutanol48
Machine-learning from Pseudomonas putida KT2440 transcriptomes reveals its transcriptional regulatory network47
Improved production of human hemoglobin in yeast by engineering hemoglobin degradation46
Microbial production of the plant-derived fungicide physcion46
Metabolic engineering of tomato fruit enriched in L-DOPA46
Engineering sub-organelles of a diploid Saccharomyces cerevisiae to enhance the production of 7-dehydrocholesterol44
Metabolic engineering of “last-line antibiotic” colistin in Paenibacillus polymyxa44
Evolution-assisted engineering of E. coli enables growth on formic acid at ambient CO2 via the Serine Threonine Cycle44
Metabolism of syringyl lignin-derived compounds in Pseudomonas putida enables convergent production of 2-pyrone-4,6-dicarboxylic acid44
Computational design and analysis of modular cells for large libraries of exchangeable product synthesis modules44
Engineering a solar formic acid/pentose (SFAP) pathway in Escherichia coli for lactic acid production42
Engineering the cellulolytic bacterium, Clostridium thermocellum, to co-utilize hemicellulose42
Rank-ordering of known enzymes as starting points for re-engineering novel substrate activity using a convolutional neural network42
Efficient production of (S)-limonene and geraniol in Saccharomyces cerevisiae through the utilization of an Erg20 mutant with enhanced GPP accumulation capability41
Fine-tuning of p-coumaric acid synthesis to increase (2S)-naringenin production in yeast41
Rational design of a bacterial import system for new-to-nature molecules41
Elucidation and engineering of Sphingolipid biosynthesis pathway in Yarrowia lipolytica for enhanced production of human-type sphingoid bases and glucosylceramides41
Yeast-based heterologous production of the Colletochlorin family of fungal secondary metabolites40
Nature as blueprint: Global phenotype engineering of CHO production cells based on a multi-omics comparison with plasma cells40
Butyrate as a growth factor of Clostridium acetobutylicum39
Optimized genome-wide CRISPR screening enables rapid engineering of growth-based phenotypes in Yarrowia lipolytica39
Biosynthesis of a rosavin natural product in Escherichia coli by glycosyltransferase rational design and artificial pathway construction39
Control of IgG glycosylation in CHO cell perfusion cultures by GReBA mathematical model supported by a novel targeted feed, TAFE38
Metabolic engineering of Synechococcus elongatus 7942 for enhanced sucrose biosynthesis38
Metabolic engineering of Escherichia coli for high production of 1,5-pentanediol via a cadaverine-derived pathway38
CRISPRi-microfluidics screening enables genome-scale target identification for high-titer protein production and secretion38
Metabolic engineering of Saccharomyces cerevisiae for de novo production of odd-numbered medium-chain fatty acids38
A genome-scale dynamic constraint-based modelling (gDCBM) framework predicts growth dynamics, medium composition and intracellular flux distributions in CHO clonal variations38
Model-guided dynamic control of essential metabolic nodes boosts acetyl-coenzyme A–dependent bioproduction in rewired Pseudomonas putida38
KETCHUP: Parameterizing of large-scale kinetic models using multiple datasets with different reference states37
Microbial synthesis of wax esters37
In-planta production of the biodegradable polyester precursor 2-pyrone-4,6-dicarboxylic acid (PDC): Stacking reduced biomass recalcitrance with value-added co-product37
Metabolic engineering of Escherichia coli for the production of an antifouling agent zosteric acid36
Kinetically guided, ratiometric tuning of fatty acid biosynthesis35
Engineering Yarrowia lipolytica for sustainable ricinoleic acid production: A pathway to free fatty acid synthesis35
Transcription factor DegU-mediated multi-pathway regulation on lichenysin biosynthesis in Bacillus licheniformis35
Corrigendum to “Engineering glucose metabolism for enhanced muconic acid production in Pseudomonas putida KT2440” [Metab. Eng. 59 (2020) 64–75]35
Synthetic metabolic pathways for conversion of CO2 into secreted short-to medium-chain hydrocarbons using cyanobacteria35
CHOGlycoNET: Comprehensive glycosylation reaction network for CHO cells34
Phenotype-centric modeling for rational metabolic engineering34
Engineering carbon source division of labor for efficient α-carotene production in Corynebacterium glutamicum33
Role of cyanobacterial phosphoketolase in energy regulation and glucose secretion under dark anaerobic and osmotic stress conditions33
Energy coupling of membrane transport and efficiency of sucrose dissimilation in yeast33
A metabolic CRISPR-Cas9 screen in Chinese hamster ovary cells identifies glutamine-sensitive genes32
Enhancing acid tolerance of Escherichia coli via viroporin-mediated export of protons and its application for efficient whole-cell biotransformation32
Alternative routes for production of the drug candidate d-chiro-inositol with Corynebacterium glutamicum using endogenous or promiscuous plant enzymes32
Metabolic engineering to produce palmitic acid or palmitoleic acid in an oleic acid-producing Corynebacterium glutamicum strain32
Combinatorial optimization and spatial remodeling of CYPs to control product profile32
Dynamic flux regulation for high-titer anthranilate production by plasmid-free, conditionally-auxotrophic strains of Pseudomonas putida32
Tuning of CHO secretional machinery improve activity of secreted therapeutic sulfatase 150-fold31
Flux balance analysis and peptide mapping elucidate the impact of bioreactor pH on Chinese hamster ovary (CHO) cell metabolism and N-linked glycosylation in the fab and Fc regions of the produced IgG31
Generation of a Vibrio-based platform for efficient conversion of raffinose through Adaptive Laboratory Evolution on a solid medium31
-Substituted 3-hydroxy acid production from glucose in Escherichia coli31
Development of a vitamin B5 hyperproducer in Escherichia coli by multiple metabolic engineering30
Comparative study of two Saccharomyces cerevisiae strains with kinetic models at genome-scale29
Improved terephthalic acid production from p-xylene using metabolically engineered Pseudomonas putida28
Overexpression of peroxisome proliferator-activated receptor co-activator-1⍺ (PGC-1⍺) in Chinese hamster ovary cells increases oxidative metabolism and IgG productivity28
Helicase-AID: A novel molecular device for base editing at random genomic loci28
A smart RBS library and its prediction model for robust and accurate fine-tuning of gene expression in Bacillus species28
Engineering low-salt growth Halomonas Bluephagenesis for cost-effective bioproduction combined with adaptive evolution28
Engineering 5-hydroxymethylfurfural (HMF) oxidation in Pseudomonas boosts tolerance and accelerates 2,5-furandicarboxylic acid (FDCA) production28
Multilevel metabolic engineering of Bacillus licheniformis for de novo biosynthesis of 2-phenylethanol28
Overproduction of medicinal ergot alkaloids based on a fungal platform27
Engineering the permeability of Halomonas bluephagenesis enhanced its chassis properties27
Active and machine learning-based approaches to rapidly enhance microbial chemical production27
Systems metabolic engineering of Corynebacterium glutamicum for high-level production of 1,3-propanediol from glucose and xylose27
De novo biosynthesis of carminic acid in Saccharomyces cerevisiae27
Organic waste conversion through anaerobic digestion: A critical insight into the metabolic pathways and microbial interactions26
Portable bacterial CRISPR transcriptional activation enables metabolic engineering in Pseudomonas putida26
Investigation of putative genes for the production of medium-chained acids and alcohols in autotrophic acetogenic bacteria26
Biosensor-informed engineering of Cupriavidus necator H16 for autotrophic D-mannitol production26
Resource allocation modeling for autonomous prediction of plant cell phenotypes26
Metabolic engineering of Saccharomyces cerevisiae for gram-scale diosgenin production26
High-level and -yield production of L-leucine in engineered Escherichia coli by multistep metabolic engineering26
Engineering Escherichia coli for production of geraniol by systematic synthetic biology approaches and laboratory-evolved fusion tags25
High efficient production of plant flavonoids by microbial cell factories: Challenges and opportunities25
Rational genome and metabolic engineering of Candida viswanathii by split CRISPR to produce hundred grams of dodecanedioic acid25
Parageobacillus thermoglucosidasius as an emerging thermophilic cell factory25
Building a highly efficient Streptomyces super-chassis for secondary metabolite production by reprogramming naturally-evolved multifaceted shifts24
A “push-pull-restrain” strategy to improve citronellol production in Saccharomyces cerevisiae24
A novel WRKY34-bZIP3 module regulates phenolic acid and tanshinone biosynthesis in Salvia miltiorrhiza24
Metabolic engineering of the malonyl-CoA pathway to efficiently produce malonate in Saccharomyces cerevisiae24
Metabolic engineering of Halomonas bluephagenesis for production of five carbon molecular chemicals derived from L-lysine24
Pushing and pulling proteins into the yeast secretory pathway enhances recombinant protein secretion24
Heterologous biosynthesis of betanin triggers metabolic reprogramming in tobacco24
Hyperproduction of 7-dehydrocholesterol by rewiring the post-squalene module in lipid droplets of Saccharomyces cerevisiae24
Synthetic translational coupling system for accurate and predictable polycistronic gene expression control in bacteria23
Engineering an SspB-mediated degron for novel controllable protein degradation23
Systematic engineering for production of anti-aging sunscreen compound in Pseudomonas putida23
De novo 2′-fucosyllactose biosynthesis using glucose as the sole carbon source by multiple engineered Bacillus subtilis23
Laboratory evolution of Synechocystis sp. PCC 6803 for phenylpropanoid production23
Editorial Board23
Optimized production of concanamycins using a rational metabolic engineering strategy23
Debottlenecking 4-hydroxybenzoate hydroxylation in Pseudomonas putida KT2440 improves muconate productivity from p-coumarate23
Efficient production of protocatechuic acid using systems engineering of Escherichia coli23
Developing a novel heme biosensor to produce high-active hemoproteins in Pichia pastoris through comparative transcriptomics22
Editorial Board22
Biosynthesis of 10-Hydroxy-2-Decenoic Acid in Escherichia coli22
Metabolic engineering of Halomonas bluephagenesis for high-level mevalonate production from glucose and acetate mixture22
Corrigendum to “Pathway discovery and engineering for cleavage of a β-1 lignin-derived biaryl compound” [Metab. Eng. 65 (2021) 1–10]22
Designing a microbial factory suited for plant chloroplast-derived enzymes to efficiently and green synthesize natural products: Capsanthin and capsorubin as examples22
Editorial Board22
Metabolic engineering of CHO cells towards cysteine prototrophy and systems analysis of the ensuing phenotype22
Integrating metabolome dynamics and process data to guide cell line selection in biopharmaceutical process development22
Evaluation of enzyme-constrained genome-scale model through metabolic engineering of anaerobic co-production of 2,3-butanediol and glycerol by Saccharomyces cerevisiae21
Mitigating transcriptional bottleneck using a constitutively active transcription factor, VP16-CREB, in mammalian cells21
Engineering Halomonas bluephagenesis for synthesis of polyhydroxybutyrate (PHB) in the presence of high nitrogen containing media21
A multiscale hybrid modelling methodology for cell cultures enabled by enzyme-constrained dynamic metabolic flux analysis under uncertainty21
Modularized Engineering of Shewanella oneidensis MR-1 for Efficient and Directional Synthesis of 5-Aminolevulinic Acid21
A manipulation of carotenoid metabolism influence biomass partitioning and fitness in tomato21
Model-based optimization of cell-free enzyme cascades exemplified for the production of GDP-fucose21
A SWEET surprise: Anaerobic fungal sugar transporters and chimeras enhance sugar uptake in yeast21
Editorial Board20
Editorial Board20
Editorial Board20
Versatile xylose and arabinose genetic switches development for yeasts20
Lepidopteran mevalonate pathway optimization in Escherichia coli efficiently produces isoprenol analogs for next-generation biofuels20
Production of cholesterol-like molecules impacts Escherichia coli robustness, production capacity, and vesicle trafficking20
Genome-scale metabolic modeling of P. thermoglucosidasius NCIMB 11955 reveals metabolic bottlenecks in anaerobic metabolism20
Production of the infant formula ingredient 1,3-olein-2-palmitin in Arabidopsis thaliana seeds20
Editorial Board20
A quantitative metabolic analysis reveals Acetobacterium woodii as a flexible and robust host for formate-based bioproduction20
A tRNA Modification-based strategy for Identifying amiNo acid Overproducers (AMINO)20
Single cell mutant selection for metabolic engineering of actinomycetes20
Compartmentalization of pathway sequential enzymes into synthetic protein compartments for metabolic flux optimization in Escherichia coli19
Design and construction of an artificial pathway for biosynthesis of acetaminophen in Escherichia coli19
Rational orthologous pathway and biochemical process engineering for adipic acid production using Pseudomonas taiwanensis VLB12019
Development of a Streptomyces-based system for facile thioholgamide library generation and analysis19
Genome-wide host-pathway interactions affecting cis-cis-muconic acid production in yeast19
Coordinated reprogramming of ATP metabolism strongly enhances adipic acid production in Escherichia coli18
Switching metabolic flux by engineering tryptophan operon-assisted CRISPR interference system in Klebsiella pneumoniae18
Inferring secretory and metabolic pathway activity from omic data with secCellFie18
Multi-level rebalancing of the naringenin pathway using riboswitch-guided high-throughput screening18
Isotopically nonstationary 13C metabolic flux analysis in resting and activated human platelets18
High-throughput insertional mutagenesis reveals novel targets for enhancing lipid accumulation in Nannochloropsis oceanica18
Metabolic flux optimization of iterative pathways through orthogonal gene expression control: Application to the β-oxidation reversal18
Synergistic experimental and computational approach identifies novel strategies for polyhydroxybutyrate overproduction17
RB-TnSeq identifies genetic targets for improved tolerance of Pseudomonas putida towards compounds relevant to lignin conversion17
Implantation of CPT1AM-expressing adipocytes reduces obesity and glucose intolerance in mice17
Glycoengineering of Aspergillus nidulans to produce precursors for humanized N-glycan structures17
Engineering acetylation platform for the total biosynthesis of D-amino acids17
Thermodynamic limitations of PHB production from formate and fructose in Cupriavidus necator17
Metabolic reprogramming of synovial fibroblasts in osteoarthritis by inhibition of pathologically overexpressed pyruvate dehydrogenase kinases17
Pathway elucidation and microbial synthesis of proaporphine and bis-benzylisoquinoline alkaloids from sacred lotus (Nelumbo nucifera)17
Identification of a specific exporter that enables high production of aconitic acid in Aspergillus pseudoterreus17
Genome-scale and pathway engineering for the sustainable aviation fuel precursor isoprenol production in Pseudomonas putida17
Multidimensional engineering of Saccharomyces cerevisiae for the efficient production of heme by exploring the cytotoxicity and tolerance of heme16
Nucleotide distribution analysis of 5′UTRs in genome-scale directs their redesign and expression regulation in yeast16
Efficient production of retinol in Yarrowia lipolytica by increasing stability using antioxidant and detergent extraction16
Engineering a powerful green cell factory for robust photoautotrophic diterpenoid production16
Reconstructing curcumin biosynthesis in yeast reveals the implication of caffeoyl-shikimate esterase in phenylpropanoid metabolic flux16
The flocculant Saccharomyces cerevisiae strain gains robustness via alteration of the cell wall hydrophobicity16
Unraveling productivity-enhancing genes in Chinese hamster ovary cells via CRISPR activation screening using recombinase-mediated cassette exchange system16
Protein engineering of an oxidative cleavage-free pathway for crocetin-dialdehyde production in Escherichia coli16
De novo production of versatile oxidized kaurene diterpenes in Escherichia coli16
Integrated knowledge mining, genome-scale modeling, and machine learning for predicting Yarrowia lipolytica bioproduction16
Deciphering the network of cholesterol biosynthesis in Paris polyphylla laid a base for efficient diosgenin production in plant chassis16
Highly efficient production of L-homoserine in Escherichia coli by engineering a redox balance route15
Systems metabolic engineering of Corynebacterium glutamicum for the efficient production of β-alanine15
Live isolation of naïve ESCs via distinct glucose metabolism and stored glycogen15
Coordinating caffeic acid and salvianic acid A pathways for efficient production of rosmarinic acid in Escherichia coli15
Guiding stars to the field of dreams: Metabolically engineered pathways and microbial platforms for a sustainable lignin-based industry15
Combinational quorum sensing devices for dynamic control in cross-feeding cocultivation15
Riboswitch-guided chalcone synthase engineering and metabolic flux optimization for enhanced production of flavonoids15
Rational search of genetic design space for a heterologous terpene metabolic pathway in Streptomyces15
Controlling selectivity of modular microbial biosynthesis of butyryl-CoA-derived designer esters15
Metabolic engineering of the oleaginous alga Nannochloropsis for enriching eicosapentaenoic acid in triacylglycerol by combined pulling and pushing strategies15
Dynamic upregulation of the rate-limiting enzyme for valerolactam biosynthesis in Corynebacterium glutamicum15
A self-regulated network for dynamically balancing multiple precursors in complex biosynthetic pathways15
Enhanced targeted DNA methylation of the CMV and endogenous promoters with dCas9-DNMT3A3L entails distinct subsequent histone modification changes in CHO cells14
Plastidial engineering with coupled farnesyl diphosphate pool reconstitution and enhancement for sesquiterpene biosynthesis in tomato fruit14
Metabolic engineering of the shikimate pathway in Amycolatopsis strains for optimized glycopeptide antibiotic production14
Food grade microbial synthesis of the butter aroma compound butanedione using engineered and non-engineered Lactococcus lactis14
Enzyme activity engineering based on sequence co-evolution analysis14
A landing pad system for multicopy gene integration in Issatchenkia orientalis14
Evolution and engineering of pathways for aromatic O-demethylation in Pseudomonas putida KT244014
13C-Metabolic flux analysis of 3T3-L1 adipocytes illuminates its core metabolism under hypoxia14
Design and application of a kinetic model of lipid metabolism in Saccharomyces cerevisiae14
Leveraging a Y. lipolytica naringenin chassis for biosynthesis of apigenin and associated glucoside13
Engineering yeast for high-level production of β-farnesene from sole methanol13
Construction of an orthogonal transport system for Saccharomyces cerevisiae peroxisome to efficiently produce sesquiterpenes13
Development of an efficient yeast platform for cannabigerolic acid biosynthesis13
A dynamic kinetic model captures cell-free metabolism for improved butanol production13
A tunable metabolic valve for precise growth control and increased product formation in Pseudomonas putida13
Reprint of Organic waste conversion through anaerobic digestion: A critical insight into the metabolic pathways and microbial interactions13
Metabolic engineering of Pichia pastoris for overproduction of cis-trans nepetalactol13
Overexpression of the transcriptional activators Mxr1 and Mit1 enhances lactic acid production on methanol in Komagataella phaffii13
Machine-learning guided elucidation of contribution of individual steps in the mevalonate pathway and construction of a yeast platform strain for terpenoid production13
Systems engineering of Escherichia coli for high-level shikimate production13
Laboratory evolution reveals general and specific tolerance mechanisms for commodity chemicals12
Metabolic flux enhancement from the translational fusion of terpene synthases is linked to terpene synthase accumulation12
Stochastic simulation algorithm for isotope-based dynamic flux analysis12
Cyanobacterial phycobilisomes as a platform for the stable production of heterologous enzymes and other proteins12
13C metabolic flux analysis clarifies distinct metabolic phenotypes of cancer cell spheroid mimicking tumor hypoxia12
Constraint-based metabolic control analysis for rational strain engineering12
13C-metabolic flux analysis of Clostridium ljungdahlii illuminates its core metabolism under mixotrophic culture conditions12
Engineering precursor supply for the high-level production of ergothioneine in Saccharomyces cerevisiae12
A counting-strategy together with a spatial structured model describes RNA polymerase and ribosome availability in Escherichia coli12
Adaptive laboratory evolution for improved tolerance of isobutyl acetate in Escherichia coli12
Integrated rational and evolutionary engineering of genome-reduced Pseudomonas putida strains promotes synthetic formate assimilation12
CelloSelect – A synthetic cellobiose metabolic pathway for selection of stable transgenic CHO cell lines12
BGC heteroexpression strategy for production of novel microbial secondary metabolites12
YaliCMulti and YaliHMulti: Stable, efficient multi-copy integration tools for engineering Yarrowia lipolytica12
Editorial Board12
Engineering metabolic flux for the microbial synthesis of aromatic compounds12
Integrating thermodynamic and enzymatic constraints into genome-scale metabolic models12
dCas9-mediated dysregulation of gene expression in human induced pluripotent stem cells during primitive streak differentiation11
Engineering of a robust Escherichia coli chassis and exploitation for large-scale production processes11
Metabolic engineering of Corynebacterium glutamicum for production of scyllo-inositol, a drug candidate against Alzheimer's disease11
Applying metabolic control strategies to engineered T cell cancer therapies11
Genetic heterogeneity of engineered Escherichia coli Nissle 1917 strains during scale-up simulation11
Short and long-read ultra-deep sequencing profiles emerging heterogeneity across five platform Escherichia coli strains11
Cascaded valorization of brown seaweed to produce l-lysine and value-added products using Corynebacterium glutamicum streamlined by systems metabolic engineering11
Metabolic engineering of Escherichia coli for N-methylserotonin biosynthesis11
Auto-inducible synthetic pathway in E. coli enhanced sustainable indigo production from glucose11
Multi-dimensional experimental and computational exploration of metabolism pinpoints complex probiotic interactions11
Unlocking lager's flavour palette by metabolic engineering of Saccharomyces pastorianus for enhanced ethyl ester production11
Paving the way for synthetic C1 - Metabolism in Pseudomonas putida through the reductive glycine pathway10
Editorial Board10
Systems metabolic engineering upgrades Corynebacterium glutamicum to high-efficiency cis, cis-muconic acid production from lignin-based aromatics10
Consistent biosynthesis of D-glycerate from variable mixed substrates10
Rethinking 13C-metabolic flux analysis – The Bayesian way of flux inference10
Metabolic engineering of E. coli for β-alanine production using a multi-biosensor enabled approach10
Editorial Board10
Optogenetic reprogramming of carbon metabolism using light-powering microbial proton pump systems10
Engineering heterologous molybdenum-cofactor-biosynthesis and nitrate-assimilation pathways enables nitrate utilization by Saccharomyces cerevisiae10
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