Metabolic Engineering

Papers
(The median citation count of Metabolic Engineering is 10. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-08-01 to 2025-08-01.)
ArticleCitations
Editorial Board133
Elucidation and engineering of Sphingolipid biosynthesis pathway in Yarrowia lipolytica for enhanced production of human-type sphingoid bases and glucosylceramides111
Alternative routes for production of the drug candidate d-chiro-inositol with Corynebacterium glutamicum using endogenous or promiscuous plant enzymes108
Microbial synthesis of wax esters104
Synthetic metabolic pathways for conversion of CO2 into secreted short-to medium-chain hydrocarbons using cyanobacteria95
Editorial Board88
Engineering Halomonas bluephagenesis for pilot production of terpolymers containing 3-hydroxybutyrate, 4-hydroxybutyrate and 3-hydroxyvalerate from glucose72
Heterologous biosynthesis of betanin triggers metabolic reprogramming in tobacco69
Computational design and analysis of modular cells for large libraries of exchangeable product synthesis modules69
Introduction of acetyl-phosphate bypass and increased culture temperatures enhanced growth-coupled poly-hydroxybutyrate production in the marine cyanobacterium Synechococcus sp. PCC700264
Biosensor-informed engineering of Cupriavidus necator H16 for autotrophic D-mannitol production63
Engineering Yarrowia lipolytica for sustainable ricinoleic acid production: A pathway to free fatty acid synthesis62
Efficient production of (S)-limonene and geraniol in Saccharomyces cerevisiae through the utilization of an Erg20 mutant with enhanced GPP accumulation capability59
Model-guided dynamic control of essential metabolic nodes boosts acetyl-coenzyme A–dependent bioproduction in rewired Pseudomonas putida59
Engineering sub-organelles of a diploid Saccharomyces cerevisiae to enhance the production of 7-dehydrocholesterol59
Engineering carbon source division of labor for efficient α-carotene production in Corynebacterium glutamicum58
Butyrate as a growth factor of Clostridium acetobutylicum56
Biosynthesis of 12-aminododecanoic acid from biomass sugars55
The flocculant Saccharomyces cerevisiae strain gains robustness via alteration of the cell wall hydrophobicity54
Optimized production of concanamycins using a rational metabolic engineering strategy54
Development of a Streptomyces-based system for facile thioholgamide library generation and analysis53
Multi-level rebalancing of the naringenin pathway using riboswitch-guided high-throughput screening53
Integrating metabolome dynamics and process data to guide cell line selection in biopharmaceutical process development50
Glycoengineering of Aspergillus nidulans to produce precursors for humanized N-glycan structures49
Inferring secretory and metabolic pathway activity from omic data with secCellFie48
13C-Metabolic flux analysis of 3T3-L1 adipocytes illuminates its core metabolism under hypoxia48
Metabolic engineering of the oleaginous alga Nannochloropsis for enriching eicosapentaenoic acid in triacylglycerol by combined pulling and pushing strategies48
Engineering an SspB-mediated degron for novel controllable protein degradation47
Synergistic experimental and computational approach identifies novel strategies for polyhydroxybutyrate overproduction45
Pathway elucidation and microbial synthesis of proaporphine and bis-benzylisoquinoline alkaloids from sacred lotus (Nelumbo nucifera)45
Identification of a specific exporter that enables high production of aconitic acid in Aspergillus pseudoterreus45
De novo 2′-fucosyllactose biosynthesis using glucose as the sole carbon source by multiple engineered Bacillus subtilis45
Quantifying supply and demand in the pea aphid-Buchnera symbiosis reveals the metabolic Achilles’ heels of this interaction45
Integrated knowledge mining, genome-scale modeling, and machine learning for predicting Yarrowia lipolytica bioproduction44
Versatile xylose and arabinose genetic switches development for yeasts44
Machine-learning guided elucidation of contribution of individual steps in the mevalonate pathway and construction of a yeast platform strain for terpenoid production44
Coordinating caffeic acid and salvianic acid A pathways for efficient production of rosmarinic acid in Escherichia coli44
Rethinking 13C-metabolic flux analysis – The Bayesian way of flux inference43
Highly efficient production of L-homoserine in Escherichia coli by engineering a redox balance route43
Silk fibroin production in Escherichia coli is limited by a positive feedback loop between metabolic burden and toxicity stress43
Metabolic engineering of Escherichia coli for N-methylserotonin biosynthesis43
Engineering diverse fatty acid compositions of phospholipids in Escherichia coli43
Advances in systems metabolic engineering of autotrophic carbon oxide-fixing biocatalysts towards a circular economy42
Consistent biosynthesis of D-glycerate from variable mixed substrates41
CelloSelect – A synthetic cellobiose metabolic pathway for selection of stable transgenic CHO cell lines41
Consolidated bioprocessing for bioethanol production by metabolically engineered cellulolytic fungus Myceliophthora thermophila40
Auto-inducible synthetic pathway in E. coli enhanced sustainable indigo production from glucose40
Engineering the thermotolerant industrial yeast Kluyveromyces marxianus for anaerobic growth40
COSMIC-dFBA: A novel multi-scale hybrid framework for bioprocess modeling40
Integrating thermodynamic and enzymatic constraints into genome-scale metabolic models39
Unlocking lager's flavour palette by metabolic engineering of Saccharomyces pastorianus for enhanced ethyl ester production39
Cascaded valorization of brown seaweed to produce l-lysine and value-added products using Corynebacterium glutamicum streamlined by systems metabolic engineering38
Magnetosome membrane engineering to improve G protein-coupled receptor activities in the magnetosome display system38
Coupling cell growth and biochemical pathway induction in Saccharomyces cerevisiae for production of (+)-valencene and its chemical conversion to (+)-nootkatone38
Boosting titers of engineered triketide and tetraketide synthases to record levels through T7 promoter tuning37
A Pseudomonas taiwanensis malonyl-CoA platform strain for polyketide synthesis37
Deep learning for NAD/NADP cofactor prediction and engineering using transformer attention analysis in enzymes37
A novel step towards the heterologous biosynthesis of paclitaxel: Characterization of T1βOH taxane hydroxylase37
A reactive species reactions module for integration into genome-scale metabolic models for improved insights: Application to cancer36
Secretory production of spider silk proteins in metabolically engineered Corynebacterium glutamicum for spinning into tough fibers36
Functional genomic screening in Komagataella phaffii enabled by high-activity CRISPR-Cas9 library36
Time-resolved, deuterium-based fluxomics uncovers the hierarchy and dynamics of sugar processing by Pseudomonas putida36
Engineering Rhodosporidium toruloides for production of 3-hydroxypropionic acid from lignocellulosic hydrolysate34
Engineering and evolution of the complete Reductive Glycine Pathway in Saccharomyces cerevisiae for formate and CO2 assimilation33
Biosynthesis of diverse α,ω-diol-derived polyhydroxyalkanoates by engineered Halomonas bluephagenesis33
Membrane transporter identification and modulation via adaptive laboratory evolution33
Metabolic engineering of commensal bacteria for gut butyrate delivery and dissection of host-microbe interaction33
Endogenous BiP reporter system for simultaneous identification of ER stress and antibody production in Chinese hamster ovary cells32
Corrigendum to “Tandem chemical deconstruction and biological upcycling of poly(ethylene terephthalate) to β-ketoadipic acid by Pseudomonas putida KT2440” (Metab. Eng. 67 (2021) 250–261)32
Establishing recombinant production of pediocin PA-1 in Corynebacterium glutamicum32
Editorial Board32
Selective microbial production of lacto-N-fucopentaose I in Escherichia coli using engineered α-1,2-fucosyltransferases32
Flux optimization using multiple promoters in Halomonas bluephagenesis as a model chassis of the next generation industrial biotechnology31
Self-controlled in silico gene knockdown strategies to enhance the sustainable production of heterologous terpenoid by Saccharomyces cerevisiae30
Systems metabolic engineering of Corynebacterium glutamicum eliminates all by-products for selective and high-yield production of the platform chemical 5-aminovalerate29
Repurposing plant hormone receptors as chemically-inducible genetic switches for dynamic regulation in yeast29
Co-cultivation of Saccharomyces cerevisiae strains combines advantages of different metabolic engineering strategies for improved ethanol yield29
Stress-driven dynamic regulation of multiple genes to reduce accumulation of toxic aldehydes29
Hybrid biological-chemical strategy for converting polyethylene into a recyclable plastic monomer using engineered Corynebacterium glutamicum29
Verazine biosynthesis from simple sugars in engineered Saccharomyces cerevisiae29
Manufacturing specialized wax esters in plants29
Targeting cofactors regeneration in methylation and hydroxylation for high level production of Ferulic acid29
Metabolic engineering of artificially modified transcription factor SmMYB36-VP16 for high-level production of tanshinones and phenolic acids29
Systems metabolic engineering upgrades Corynebacterium glutamicum for selective high-level production of the chiral drug precursor and cell-protective extremolyte L-pipecolic acid29
Development of a growth coupled and multi-layered dynamic regulation network balancing malonyl-CoA node to enhance (2S)-naringenin biosynthesis in Escherichia coli28
Engineering of insect juvenile hormone III biosynthesis in the plant Nicotiana benthamiana28
Efficient production of natural sunscreens shinorine, porphyra-334, and mycosporine-2-glycine in Saccharomyces cerevisiae28
Evaluation of strategies to narrow the product chain-length distribution of microbially synthesized free fatty acids28
Fluctuating pH for efficient photomixotrophic succinate production27
Electrical-biological hybrid system for carbon efficient isobutanol production27
High-yield production of FK228 and new derivatives in a Burkholderia chassis27
Kinetically guided, ratiometric tuning of fatty acid biosynthesis27
Ectoine hyperproduction by engineered Halomonas bluephagenesis27
Editorial Board27
Developing a Bacillus licheniformis platform for de novo production of γ-aminobutyric acid and other glutamate-derived chemicals27
Overexpression of peroxisome proliferator-activated receptor co-activator-1⍺ (PGC-1⍺) in Chinese hamster ovary cells increases oxidative metabolism and IgG productivity26
Engineering 5-hydroxymethylfurfural (HMF) oxidation in Pseudomonas boosts tolerance and accelerates 2,5-furandicarboxylic acid (FDCA) production26
CRISPRi-microfluidics screening enables genome-scale target identification for high-titer protein production and secretion26
Metabolic engineering of Saccharomyces cerevisiae for gram-scale diosgenin production26
Minimized antibiotic-free plasmid vector for gene therapy utilizing a new toxin-antitoxin system25
Generation of a Vibrio-based platform for efficient conversion of raffinose through Adaptive Laboratory Evolution on a solid medium25
Evolution-assisted engineering of E. coli enables growth on formic acid at ambient CO2 via the Serine Threonine Cycle25
Biosynthesis of β-lactam nuclei in yeast25
Engineering the permeability of Halomonas bluephagenesis enhanced its chassis properties25
Metabolic engineering of Escherichia coli for high production of 1,5-pentanediol via a cadaverine-derived pathway25
Rational search of genetic design space for a heterologous terpene metabolic pathway in Streptomyces24
Genetic and biocatalytic basis of formate dependent growth of Escherichia coli strains evolved in continuous culture24
KETCHUP: Parameterizing of large-scale kinetic models using multiple datasets with different reference states24
Rational orthologous pathway and biochemical process engineering for adipic acid production using Pseudomonas taiwanensis VLB12024
Unraveling productivity-enhancing genes in Chinese hamster ovary cells via CRISPR activation screening using recombinase-mediated cassette exchange system24
High-level and -yield production of L-leucine in engineered Escherichia coli by multistep metabolic engineering24
Sustainable production of genistin from glycerol by constructing and optimizing Escherichia coli24
Coordinated reprogramming of ATP metabolism strongly enhances adipic acid production in Escherichia coli24
Editorial Board24
Organic waste conversion through anaerobic digestion: A critical insight into the metabolic pathways and microbial interactions24
Thermodynamic limitations of PHB production from formate and fructose in Cupriavidus necator24
A tRNA Modification-based strategy for Identifying amiNo acid Overproducers (AMINO)23
Debottlenecking 4-hydroxybenzoate hydroxylation in Pseudomonas putida KT2440 improves muconate productivity from p-coumarate23
A dynamic kinetic model captures cell-free metabolism for improved butanol production23
Efficient production of retinol in Yarrowia lipolytica by increasing stability using antioxidant and detergent extraction23
Development of an efficient yeast platform for cannabigerolic acid biosynthesis23
Metabolic engineering of Corynebacterium glutamicum for production of scyllo-inositol, a drug candidate against Alzheimer's disease22
Multi-omic based production strain improvement (MOBpsi) for bio-manufacturing of toxic chemicals22
Editorial Board22
A tunable metabolic valve for precise growth control and increased product formation in Pseudomonas putida22
dCas9-mediated dysregulation of gene expression in human induced pluripotent stem cells during primitive streak differentiation22
AI-based automated construction of high-precision Geobacillus thermoglucosidasius enzyme constraint model22
Laboratory evolution reveals general and specific tolerance mechanisms for commodity chemicals22
Genome-scale and pathway engineering for the sustainable aviation fuel precursor isoprenol production in Pseudomonas putida22
Enabling anaerobic growth of Escherichia coli on glycerol in defined minimal medium using acetate as redox sink22
Cyanobacterial phycobilisomes as a platform for the stable production of heterologous enzymes and other proteins22
Laboratory evolution of Synechocystis sp. PCC 6803 for phenylpropanoid production22
A manipulation of carotenoid metabolism influence biomass partitioning and fitness in tomato22
Engineering metabolic flux for the microbial synthesis of aromatic compounds21
Big data and machine learning: Metabolic engineering special issue21
Combinatorial iterative method for metabolic engineering of Yarrowia lipolytica: Application for betanin biosynthesis21
Integrated rational and evolutionary engineering of genome-reduced Pseudomonas putida strains promotes synthetic formate assimilation21
Multi-dimensional experimental and computational exploration of metabolism pinpoints complex probiotic interactions21
Engineering Komagataella phaffii to produce lycopene sustainably from glucose or methanol21
Engineering nucleotide sugar synthesis pathways for independent and simultaneous modulation of N-glycan galactosylation and fucosylation in CHO cells21
Engineering ethanologenicity into the extremely thermophilic bacterium Anaerocellum (f. Caldicellulosiriuptor) bescii21
Editorial Board21
Accurate prediction of in vivo protein abundances by coupling constraint-based modelling and machine learning21
A nutrition algorithm to optimize feed and medium composition using genome-scale metabolic models21
Population genomics-guided engineering of phenazine biosynthesis in Pseudomonas chlororaphis20
Evaluating proteome allocation of Saccharomyces cerevisiae phenotypes with resource balance analysis20
Metabolic engineering improves transduction efficiency and downstream vector isolation by altering the lipid composition of extracellular vesicle-enclosed AAV20
Engineering Escherichia coli for efficient glutathione production20
Metabolic engineering of human gut microbiome: Recent developments and future perspectives20
Metabolic reprogramming and biosensor-assisted mutagenesis screening for high-level production of L-arginine in Escherichia coli19
Metabolic impact of heterologous protein production in Pseudomonas putida: Insights into carbon and energy flux control19
Recent advances toward the bioconversion of methane and methanol in synthetic methylotrophs19
Establishing a coumarin production platform by protein and metabolic engineering19
Multi-omics view of recombinant Yarrowia lipolytica: Enhanced ketogenic amino acid catabolism increases polyketide-synthase-driven docosahexaenoic production to high selectivity at the gram scale19
Suppressors of amyloid-β toxicity improve recombinant protein production in yeast by reducing oxidative stress and tuning cellular metabolism19
Development of platforms for functional characterization and production of phenazines using a multi-chassis approach via CRAGE18
High-yield production of coenzyme F420 in Escherichia coli by fluorescence-based screening of multi-dimensional gene expression space18
One-pot selective biosynthesis of Tyrian purple in Escherichia coli18
Improving the soluble expression of difficult-to-express proteins in prokaryotic expression system via protein engineering and synthetic biology strategies18
Production of species-specific anthocyanins through an inducible system in plant hairy roots18
Optogenetic control of Cdc48 for dynamic metabolic engineering in yeast18
Deciphering molecular drivers of lactate metabolic shift in mammalian cell cultures18
Strategic engineering for overproduction of oviedomycin, a Type II polyketide, in Escherichia coli18
Efforts to install a heterologous Wood-Ljungdahl pathway in Clostridium acetobutylicum enable the identification of the native tetrahydrofolate (THF) cycle and result in early induction of solvents18
Bioconversion of CO2 into valuable bioproducts via synthetic modular co-culture of engineered Chlamydomonas reinhardtii and Escherichia coli18
A contribution of metabolic engineering to addressing medical problems: Metabolic flux analysis18
Dynamic regulation combined with systematic metabolic engineering for high-level palmitoleic acid accumulation in oleaginous yeast17
Editorial Board17
Chemical-damage MINE: A database of curated and predicted spontaneous metabolic reactions17
Metabolic division engineering of Escherichia coli consortia for de novo biosynthesis of flavonoids and flavonoid glycosides17
Adaptive laboratory evolution of Escherichia coli W enhances gamma-aminobutyric acid production using glycerol as the carbon source17
Rational design of a bacterial import system for new-to-nature molecules16
Cross-regulation between proteome reallocation and metabolic flux redistribution governs bacterial growth transition kinetics16
Development of Corynebacterium glutamicum as a monoterpene production platform16
Designing glucose utilization "highway" for recombinant biosynthesis16
Construction of a synthetic metabolic pathway for biosynthesis of threonine from ethylene glycol16
Editorial Board16
Systematic identification of Ocimum sanctum sesquiterpenoid synthases and (−)-eremophilene overproduction in engineered yeast16
Microbial production of creatine using growth-coupled selection systems.16
Metabolic flux analysis of the non-transitory starch tradeoff for lipid production in mature tobacco leaves16
Adaptive laboratory evolution and genetic engineering improved terephthalate utilization in Pseudomonas putida KT244016
Engineering yeast for high-level production of diterpenoid sclareol16
De novo biosynthesis of 3-hydroxy-3-methylbutyrate as anti-catabolic supplement by metabolically engineered Escherichia coli16
Modular, synthetic chromosomes as new tools for large scale engineering of metabolism16
Genome-scale models of metabolism and expression predict the metabolic burden of recombinant protein expression16
Establishing an Autonomous Cascaded Artificial Dynamic (AutoCAD) regulation system for improved pathway performance16
Directed evolution of biomass intensive CHO cells by adaptation to sub-physiological temperature15
A genome-scale dynamic constraint-based modelling (gDCBM) framework predicts growth dynamics, medium composition and intracellular flux distributions in CHO clonal variations15
Rank-ordering of known enzymes as starting points for re-engineering novel substrate activity using a convolutional neural network15
Improved terephthalic acid production from p-xylene using metabolically engineered Pseudomonas putida15
Corrigendum to “Engineering glucose metabolism for enhanced muconic acid production in Pseudomonas putida KT2440” [Metab. Eng. 59 (2020) 64–75]15
Reconstructing a recycling and nonauxotroph biosynthetic pathway in Escherichia coli toward highly efficient production of L-citrulline15
Helicase-AID: A novel molecular device for base editing at random genomic loci15
High efficient production of plant flavonoids by microbial cell factories: Challenges and opportunities15
Engineering the cellulolytic bacterium, Clostridium thermocellum, to co-utilize hemicellulose15
Switching the yeast metabolism via manipulation of sugar phosphorylation15
Engineering low-salt growth Halomonas Bluephagenesis for cost-effective bioproduction combined with adaptive evolution15
Efficient production of an antitumor precursor actinocin and other medicinal molecules from kynurenine pathway in Escherichia coli15
Multilevel metabolic engineering of Bacillus licheniformis for de novo biosynthesis of 2-phenylethanol15
Biosynthesis of a rosavin natural product in Escherichia coli by glycosyltransferase rational design and artificial pathway construction15
Metabolic engineering of Halomonas bluephagenesis for production of five carbon molecular chemicals derived from L-lysine15
Evaluation of enzyme-constrained genome-scale model through metabolic engineering of anaerobic co-production of 2,3-butanediol and glycerol by Saccharomyces cerevisiae14
Multidimensional engineering of Saccharomyces cerevisiae for the efficient production of heme by exploring the cytotoxicity and tolerance of heme14
Combinational quorum sensing devices for dynamic control in cross-feeding cocultivation14
A self-regulated network for dynamically balancing multiple precursors in complex biosynthetic pathways14
Exploring the versatility of fatty acid biosynthesis in Escherichia coli: Production of random methyl branched fatty acids14
Initiation of fatty acid biosynthesis in Pseudomonas putida KT244014
Modularized Engineering of Shewanella oneidensis MR-1 for Efficient and Directional Synthesis of 5-Aminolevulinic Acid14
Production of the infant formula ingredient 1,3-olein-2-palmitin in Arabidopsis thaliana seeds14
Riboswitch-guided chalcone synthase engineering and metabolic flux optimization for enhanced production of flavonoids14
Evolution and engineering of pathways for aromatic O-demethylation in Pseudomonas putida KT244014
Editorial Board14
Design and application of a kinetic model of lipid metabolism in Saccharomyces cerevisiae14
Live isolation of naïve ESCs via distinct glucose metabolism and stored glycogen14
Deciphering the network of cholesterol biosynthesis in Paris polyphylla laid a base for efficient diosgenin production in plant chassis14
Enzyme activity engineering based on sequence co-evolution analysis14
Food grade microbial synthesis of the butter aroma compound butanedione using engineered and non-engineered Lactococcus lactis13
The halo of future bio-industry based on engineering Halomonas13
Stochastic simulation algorithm for isotope-based dynamic flux analysis13
A counting-strategy together with a spatial structured model describes RNA polymerase and ribosome availability in Escherichia coli13
Reconstructing curcumin biosynthesis in yeast reveals the implication of caffeoyl-shikimate esterase in phenylpropanoid metabolic flux13
Engineering tropane alkaloid production and glyphosate resistance by overexpressing AbCaM1 and G2-EPSPS in Atropa belladonna13
CIFR (Clone–Integrate–Flip-out–Repeat): A toolset for iterative genome and pathway engineering of Gram-negative bacteria13
Production of cholesterol-like molecules impacts Escherichia coli robustness, production capacity, and vesicle trafficking13
Plastidial engineering with coupled farnesyl diphosphate pool reconstitution and enhancement for sesquiterpene biosynthesis in tomato fruit13
Mechanism and engineering of endoplasmic reticulum-localized membrane protein folding in Saccharomyces cerevisiae13
Systems metabolic engineering upgrades Corynebacterium glutamicum to high-efficiency cis, cis-muconic acid production from lignin-based aromatics13
Development of a nonauxotrophic L-homoserine hyperproducer in Escherichia coli by systems metabolic engineering12
Editorial Board12
Guidelines for extracting biologically relevant context-specific metabolic models using gene expression data12
A long-term growth stable Halomonas sp. deleted with multiple transposases guided by its metabolic network model Halo-ecGEM12
Metabolic engineering for the utilization of carbohydrate portions of lignocellulosic biomass12
Unleashing the innate ability of Escherichia coli to produce D-Allose12
Biosensor-based growth-coupling and spatial separation as an evolution strategy to improve small molecule production of Corynebacterium glutamicum12
The 6-phosphofructokinase reaction in Acetivibrio thermocellus is both ATP- and pyrophosphate-dependent12
Engineering of Corynebacterium glutamicum for high-level γ-aminobutyric acid production from glycerol by dynamic metabolic control12
Cycling between growth and production phases increases cyanobacteria bioproduction of lactate12
Engineering the biological conversion of formate into crotonate in Cupriavidus necator12
The metabolic potential of plastics as biotechnological carbon sources – Review and targets for the future12
Metabolic engineering of the acid-tolerant yeast Pichia kudriavzevii for efficient L-malic acid production at low pH12
Expression in CHO cells of a bacterial biosynthetic pathway producing a small non-ribosomal peptide aldehyde prevents proteolysis of recombinant proteins12
Elucidation of triacylglycerol catabolism in Yarrowia lipolytica: How cells balance acetyl-CoA and excess reducing equivalents12
Optogenetic reprogramming of carbon metabolism using light-powering microbial proton pump systems12
Top-down synthetic biology approach for titer improvement of clinically important antibiotic daptomycin in Streptomyces roseosporus12
Combinatorial gene inactivation of aldehyde dehydrogenases mitigates aldehyde oxidation catalyzed by E. coli resting cells12
Editorial Board11
Corynebacterium glutamicum cell factory design for the efficient production of cis, cis-muconic acid11
Establishing a versatile toolkit of flux enhanced strains and cell extracts for pathway prototyping11
Multiplex genome editing of mammalian cells for producing recombinant heparin11
Biosynthesis mechanism, genome mining and artificial construction of echinocandin O-sulfonation11
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