OOIR: Observatory of International Research

Papers

(The TQCC of Metabolic Engineering is 21. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-06-01 to 2025-06-01.)

Article	Citations
Editorial Board	211
Editorial Board	127
Introduction of acetyl-phosphate bypass and increased culture temperatures enhanced growth-coupled poly-hydroxybutyrate production in the marine cyanobacterium Synechococcus sp. PCC7002	107
Elucidation and engineering of Sphingolipid biosynthesis pathway in Yarrowia lipolytica for enhanced production of human-type sphingoid bases and glucosylceramides	106
Alternative routes for production of the drug candidate d-chiro-inositol with Corynebacterium glutamicum using endogenous or promiscuous plant enzymes	100
Microbial synthesis of wax esters	93
Model-guided dynamic control of essential metabolic nodes boosts acetyl-coenzyme A–dependent bioproduction in rewired Pseudomonas putida	85
Computational design and analysis of modular cells for large libraries of exchangeable product synthesis modules	68
Engineering Escherichia coli for production of geraniol by systematic synthetic biology approaches and laboratory-evolved fusion tags	67
Portable bacterial CRISPR transcriptional activation enables metabolic engineering in Pseudomonas putida	65
Efficient production of (S)-limonene and geraniol in Saccharomyces cerevisiae through the utilization of an Erg20 mutant with enhanced GPP accumulation capability	65
In-planta production of the biodegradable polyester precursor 2-pyrone-4,6-dicarboxylic acid (PDC): Stacking reduced biomass recalcitrance with value-added co-product	61
Synthetic metabolic pathways for conversion of CO2 into secreted short-to medium-chain hydrocarbons using cyanobacteria	61
A metabolic CRISPR-Cas9 screen in Chinese hamster ovary cells identifies glutamine-sensitive genes	59
Heterologous biosynthesis of betanin triggers metabolic reprogramming in tobacco	57
Butyrate as a growth factor of Clostridium acetobutylicum	55
Engineering sub-organelles of a diploid Saccharomyces cerevisiae to enhance the production of 7-dehydrocholesterol	54
Engineering Yarrowia lipolytica for sustainable ricinoleic acid production: A pathway to free fatty acid synthesis	54
Biosensor-informed engineering of Cupriavidus necator H16 for autotrophic D-mannitol production	53
The flocculant Saccharomyces cerevisiae strain gains robustness via alteration of the cell wall hydrophobicity	51
A “push-pull-restrain” strategy to improve citronellol production in Saccharomyces cerevisiae	51
Engineering carbon source division of labor for efficient α-carotene production in Corynebacterium glutamicum	51
13C-Metabolic flux analysis of 3T3-L1 adipocytes illuminates its core metabolism under hypoxia	50
Biosynthesis of 12-aminododecanoic acid from biomass sugars	50
Optimized production of concanamycins using a rational metabolic engineering strategy	50

Multi-level rebalancing of the naringenin pathway using riboswitch-guided high-throughput screening	50
Development of a Streptomyces-based system for facile thioholgamide library generation and analysis	49
Inferring secretory and metabolic pathway activity from omic data with secCellFie	47
Engineering an SspB-mediated degron for novel controllable protein degradation	47
Integrating metabolome dynamics and process data to guide cell line selection in biopharmaceutical process development	46
Pathway elucidation and microbial synthesis of proaporphine and bis-benzylisoquinoline alkaloids from sacred lotus (Nelumbo nucifera)	46
Identification of a specific exporter that enables high production of aconitic acid in Aspergillus pseudoterreus	46
Integrated knowledge mining, genome-scale modeling, and machine learning for predicting Yarrowia lipolytica bioproduction	44
Machine-learning guided elucidation of contribution of individual steps in the mevalonate pathway and construction of a yeast platform strain for terpenoid production	44
Highly efficient production of L-homoserine in Escherichia coli by engineering a redox balance route	44
Versatile xylose and arabinose genetic switches development for yeasts	44
Enhanced targeted DNA methylation of the CMV and endogenous promoters with dCas9-DNMT3A3L entails distinct subsequent histone modification changes in CHO cells	43
Glycoengineering of Aspergillus nidulans to produce precursors for humanized N-glycan structures	42
Metabolic engineering of the oleaginous alga Nannochloropsis for enriching eicosapentaenoic acid in triacylglycerol by combined pulling and pushing strategies	42
Coordinating caffeic acid and salvianic acid A pathways for efficient production of rosmarinic acid in Escherichia coli	41
Cascaded valorization of brown seaweed to produce l-lysine and value-added products using Corynebacterium glutamicum streamlined by systems metabolic engineering	41
Synergistic experimental and computational approach identifies novel strategies for polyhydroxybutyrate overproduction	41
De novo 2′-fucosyllactose biosynthesis using glucose as the sole carbon source by multiple engineered Bacillus subtilis	41
High-yield production of (R)-acetoin in Saccharomyces cerevisiae by deleting genes for NAD(P)H-dependent ketone reductases producing meso-2,3-butanediol and 2,3-dimethylglycerate	41
Silk fibroin production in Escherichia coli is limited by a positive feedback loop between metabolic burden and toxicity stress	40
Engineering diverse fatty acid compositions of phospholipids in Escherichia coli	40
Metabolic engineering of Escherichia coli for N-methylserotonin biosynthesis	40
Advances in systems metabolic engineering of autotrophic carbon oxide-fixing biocatalysts towards a circular economy	40
Rethinking 13C-metabolic flux analysis – The Bayesian way of flux inference	40
Identification of novel metabolic engineering targets for S-adenosyl-L-methionine production in Saccharomyces cerevisiae via genome-scale engineering	40
COSMIC-dFBA: A novel multi-scale hybrid framework for bioprocess modeling	39
Engineering the thermotolerant industrial yeast Kluyveromyces marxianus for anaerobic growth	39
Auto-inducible synthetic pathway in E. coli enhanced sustainable indigo production from glucose	39
Consistent biosynthesis of D-glycerate from variable mixed substrates	39
Coupling cell growth and biochemical pathway induction in Saccharomyces cerevisiae for production of (+)-valencene and its chemical conversion to (+)-nootkatone	39
Unlocking lager's flavour palette by metabolic engineering of Saccharomyces pastorianus for enhanced ethyl ester production	39
CelloSelect – A synthetic cellobiose metabolic pathway for selection of stable transgenic CHO cell lines	39
Consolidated bioprocessing for bioethanol production by metabolically engineered cellulolytic fungus Myceliophthora thermophila	39
Magnetosome membrane engineering to improve G protein-coupled receptor activities in the magnetosome display system	37
Integrating thermodynamic and enzymatic constraints into genome-scale metabolic models	37
Boosting titers of engineered triketide and tetraketide synthases to record levels through T7 promoter tuning	36
A reactive species reactions module for integration into genome-scale metabolic models for improved insights: Application to cancer	36
A Pseudomonas taiwanensis malonyl-CoA platform strain for polyketide synthesis	35
Optimization of heterologous Darobactin A expression and identification of the minimal biosynthetic gene cluster	35
Functional genomic screening in Komagataella phaffii enabled by high-activity CRISPR-Cas9 library	35
Deep learning for NAD/NADP cofactor prediction and engineering using transformer attention analysis in enzymes	35
A novel step towards the heterologous biosynthesis of paclitaxel: Characterization of T1βOH taxane hydroxylase	34
Secretory production of spider silk proteins in metabolically engineered Corynebacterium glutamicum for spinning into tough fibers	34
Biosynthesis of diverse α,ω-diol-derived polyhydroxyalkanoates by engineered Halomonas bluephagenesis	34
Time-resolved, deuterium-based fluxomics uncovers the hierarchy and dynamics of sugar processing by Pseudomonas putida	34
Membrane transporter identification and modulation via adaptive laboratory evolution	34
Engineering Rhodosporidium toruloides for production of 3-hydroxypropionic acid from lignocellulosic hydrolysate	33
Engineering and evolution of the complete Reductive Glycine Pathway in Saccharomyces cerevisiae for formate and CO2 assimilation	33
Metabolic engineering of commensal bacteria for gut butyrate delivery and dissection of host-microbe interaction	33
Systems metabolic engineering of Corynebacterium glutamicum eliminates all by-products for selective and high-yield production of the platform chemical 5-aminovalerate	31

Editorial Board	31
Corrigendum to “Tandem chemical deconstruction and biological upcycling of poly(ethylene terephthalate) to β-ketoadipic acid by Pseudomonas putida KT2440” (Metab. Eng. 67 (2021) 250–261)	31
Repurposing plant hormone receptors as chemically-inducible genetic switches for dynamic regulation in yeast	31
Self-controlled in silico gene knockdown strategies to enhance the sustainable production of heterologous terpenoid by Saccharomyces cerevisiae	31
Co-cultivation of Saccharomyces cerevisiae strains combines advantages of different metabolic engineering strategies for improved ethanol yield	31
Flux optimization using multiple promoters in Halomonas bluephagenesis as a model chassis of the next generation industrial biotechnology	30
Manufacturing specialized wax esters in plants	30
Verazine biosynthesis from simple sugars in engineered Saccharomyces cerevisiae	30
Metabolic engineering of artificially modified transcription factor SmMYB36-VP16 for high-level production of tanshinones and phenolic acids	29
Endogenous BiP reporter system for simultaneous identification of ER stress and antibody production in Chinese hamster ovary cells	29
Establishing recombinant production of pediocin PA-1 in Corynebacterium glutamicum	29
Targeting cofactors regeneration in methylation and hydroxylation for high level production of Ferulic acid	29
Selective microbial production of lacto-N-fucopentaose I in Escherichia coli using engineered α-1,2-fucosyltransferases	29
Systems metabolic engineering upgrades Corynebacterium glutamicum for selective high-level production of the chiral drug precursor and cell-protective extremolyte L-pipecolic acid	29
Ectoine hyperproduction by engineered Halomonas bluephagenesis	28
High-yield production of FK228 and new derivatives in a Burkholderia chassis	28
Engineering of insect juvenile hormone III biosynthesis in the plant Nicotiana benthamiana	28
Developing a Bacillus licheniformis platform for de novo production of γ-aminobutyric acid and other glutamate-derived chemicals	27
Development of a growth coupled and multi-layered dynamic regulation network balancing malonyl-CoA node to enhance (2S)-naringenin biosynthesis in Escherichia coli	27
Evaluation of strategies to narrow the product chain-length distribution of microbially synthesized free fatty acids	27
Electrical-biological hybrid system for carbon efficient isobutanol production	27
Engineering promiscuity of chloramphenicol acetyltransferase for microbial designer ester biosynthesis	27
Biosynthesis of β-lactam nuclei in yeast	26
Metabolic engineering of Escherichia coli for high production of 1,5-pentanediol via a cadaverine-derived pathway	26
Kinetically guided, ratiometric tuning of fatty acid biosynthesis	26
Genetic and biocatalytic basis of formate dependent growth of Escherichia coli strains evolved in continuous culture	26
Efficient production of natural sunscreens shinorine, porphyra-334, and mycosporine-2-glycine in Saccharomyces cerevisiae	26
KETCHUP: Parameterizing of large-scale kinetic models using multiple datasets with different reference states	26
Fluctuating pH for efficient photomixotrophic succinate production	26
Editorial Board	26
Evolution-assisted engineering of E. coli enables growth on formic acid at ambient CO2 via the Serine Threonine Cycle	25
Overexpression of peroxisome proliferator-activated receptor co-activator-1⍺ (PGC-1⍺) in Chinese hamster ovary cells increases oxidative metabolism and IgG productivity	25
Sustainable production of genistin from glycerol by constructing and optimizing Escherichia coli	25
Minimized antibiotic-free plasmid vector for gene therapy utilizing a new toxin-antitoxin system	25
Engineering the permeability of Halomonas bluephagenesis enhanced its chassis properties	25
Generation of a Vibrio-based platform for efficient conversion of raffinose through Adaptive Laboratory Evolution on a solid medium	25
CRISPRi-microfluidics screening enables genome-scale target identification for high-titer protein production and secretion	25
Engineering 5-hydroxymethylfurfural (HMF) oxidation in Pseudomonas boosts tolerance and accelerates 2,5-furandicarboxylic acid (FDCA) production	25
Metabolic engineering of Saccharomyces cerevisiae for gram-scale diosgenin production	24
Editorial Board	24
Unraveling productivity-enhancing genes in Chinese hamster ovary cells via CRISPR activation screening using recombinase-mediated cassette exchange system	24
Efficient production of retinol in Yarrowia lipolytica by increasing stability using antioxidant and detergent extraction	24
Development of an efficient yeast platform for cannabigerolic acid biosynthesis	24
Organic waste conversion through anaerobic digestion: A critical insight into the metabolic pathways and microbial interactions	24
A tRNA Modification-based strategy for Identifying amiNo acid Overproducers (AMINO)	24
A tunable metabolic valve for precise growth control and increased product formation in Pseudomonas putida	24
A manipulation of carotenoid metabolism influence biomass partitioning and fitness in tomato	24
High-level and -yield production of L-leucine in engineered Escherichia coli by multistep metabolic engineering	24
Editorial Board	24
Coordinated reprogramming of ATP metabolism strongly enhances adipic acid production in Escherichia coli	24
Rational search of genetic design space for a heterologous terpene metabolic pathway in Streptomyces	24
Debottlenecking 4-hydroxybenzoate hydroxylation in Pseudomonas putida KT2440 improves muconate productivity from p-coumarate	23
Rational orthologous pathway and biochemical process engineering for adipic acid production using Pseudomonas taiwanensis VLB120	23
A dynamic kinetic model captures cell-free metabolism for improved butanol production	23
Laboratory evolution of Synechocystis sp. PCC 6803 for phenylpropanoid production	23
Genome-scale and pathway engineering for the sustainable aviation fuel precursor isoprenol production in Pseudomonas putida	22
Cyanobacterial phycobilisomes as a platform for the stable production of heterologous enzymes and other proteins	22
Laboratory evolution reveals general and specific tolerance mechanisms for commodity chemicals	22
Editorial Board	22
Thermodynamic limitations of PHB production from formate and fructose in Cupriavidus necator	22
AI-based automated construction of high-precision Geobacillus thermoglucosidasius enzyme constraint model	22
Metabolic engineering of Corynebacterium glutamicum for production of scyllo-inositol, a drug candidate against Alzheimer's disease	21
Microbial production of human milk oligosaccharide lactodifucotetraose	21
Animal-free heme production for artificial meat in Corynebacterium glutamicum via systems metabolic and membrane engineering	21
Multi-dimensional experimental and computational exploration of metabolism pinpoints complex probiotic interactions	21
Enabling anaerobic growth of Escherichia coli on glycerol in defined minimal medium using acetate as redox sink	21
Multi-omic based production strain improvement (MOBpsi) for bio-manufacturing of toxic chemicals	21
Engineering ethanologenicity into the extremely thermophilic bacterium Anaerocellum (f. Caldicellulosiriuptor) bescii	21
Engineering metabolic flux for the microbial synthesis of aromatic compounds	21
dCas9-mediated dysregulation of gene expression in human induced pluripotent stem cells during primitive streak differentiation	21
Integrated rational and evolutionary engineering of genome-reduced Pseudomonas putida strains promotes synthetic formate assimilation	21