BMC Biotechnology

Article	Citations
Improvement and prediction of the extraction parameters of lupeol and stigmasterol metabolites of Melia azedarach with response surface methodology	60
Simplified cloning and isolation of peptides from “sandwiched” SUMO-peptide-intein fusion proteins	40
Identification of a novel fully human anti-toxic shock syndrome toxin (TSST)-1 single-chain variable fragment antibody averting TSST-1-induced mitogenesis and cytokine secretion	39
Parabacteroides distasonis promotes CXCL9 secretion of tumor-associated macrophages and enhances CD8+T cell activity to trigger anti-tumor immunity against anti-PD-1 treatment in non-small cell lung c	38
Optimized zymogram protocol from 3D spheroid cultures to study MMP-2 and -9 activities in tumor cells	34
Biocompatibility and potential anticancer activity of gadolinium oxide (Gd2O3) nanoparticles against nasal squamous cell carcinoma	33
3D printing of Ceffe-infused scaffolds for tailored nipple-like cartilage development	31
Maximizing lipid accumulation in Tetradesmus obliquus under heavy metal stress for sustainable biodiesel innovation	31
The mechanism of degradation of alizarin red by a white-rot fungus Trametes gibbosa	29
Enzymatic degradation is an effective means to reduce aflatoxin contamination in maize	26
Reductive enzymatic dynamic kinetic resolution affording 115 g/L (S)-2-phenylpropanol	24
Synergistic effect of zinc oxide-cinnamic acid nanoparticles for wound healing management: in vitro and zebrafish model studies	23
Biosafety and pharmacokinetic characteristics of polyethylene pyrrolidone modified nano selenium in rats	23
Electrospun PCL membranes for localized drug delivery and bone regeneration	20
Transgenic Arabidopsis thaliana plants expressing bacterial γ-hexachlorocyclohexane dehydrochlorinase LinA	20
Rice yellow mottle virus is a suitable amplicon vector for an efficient production of an anti-leishmianiasis vaccine in Nicotiana benthamiana leaves	19
Fusarium verticillioides pigment: production, response surface optimization, gamma irradiation and encapsulation studies	18
Evaluating the anticancer efficacy of Chara vulgaris ethanolic extract and selenium nanoformulation in Ehrlich carcinoma mice: role of autophagy and apoptosis	18
Fabrication and characterization of metformin-loaded PLGA/Collagen nanofibers for modulation of macrophage polarization for tissue engineering and regenerative medicine	17
Optimization of lipase production using fungal isolates from oily residues	17
Comparison of vector elements and process conditions in transient and stable suspension HEK293 platforms using SARS-CoV-2 receptor binding domain as a model protein	17
Biochemical characterization and insights into the potency of the acidic Aspergillus niger NRC114 purified α-galactosidase in removing raffinose family oligosaccharides from soymilk yogurt	17
Highly multiplexed targeted sequencing strategy for infectious disease surveillance	17
A new mRNA structure prediction based approach to identifying improved signal peptides for bone morphogenetic protein 2	16
Soft sensor modeling method for Pichia pastoris fermentation process based on substructure domain transfer learning	15

Purification and characterization of soluble recombinant Crimean-Congo hemorrhagic fever virus glycoprotein Gc expressed in mammalian 293F cells	15
Chitosan nano-formulation enhances stability and bactericidal activity of the lytic phage HK6	15
Generation of a recombinant antibody for sensitive detection of Pseudomonas aeruginosa	14
Newly designed curcumin-loaded hybrid nanoparticles: a multifunctional strategy for combating oxidative stress, inflammation, and infections to accelerate wound healing and tissue regeneration	14
One-step fermentation for producing xylo-oligosaccharides from wheat bran by recombinant Escherichia coli containing an alkaline xylanase	13
The ESRP1 promoter reporter can function as an in vivo sensor of DNA methyltransferase inhibition	13
A critical role for erythropoietin on vagus nerve Schwann cells in intestinal motility	12
Anti-inflammatory potential of aspergillus unguis SP51-EGY: TLR4-dependent effects & chemical diversity via Q-TOF LC-HRMS	12
Molecular and agro-morphological characterization of new barley genotypes in arid environments	12
Central position of histidine in the sequence of designed alternating polarity peptides enhances pH-responsive assembly with DNA	12
Gene cloning and molecular characterization of a thermostable chitosanase from Bacillus cereus TY24	11
A novel allogeneic acellular matrix scaffold for porcine cartilage regeneration	11
Isolation and identification of antifungal, antibacterial and nematocide agents from marine bacillus gottheilii MSB1	11
Aripiprazole-loaded niosome/chitosan-gold nanoparticles for breast cancer chemo-photo therapy	10
A copper switch for inducing CRISPR/Cas9-based transcriptional activation tightly regulates gene expression in Nicotiana benthamiana	10
Unlocking the antioxidant and antibacterial potential of exopolysaccharides produced by endophytic fungi (Aspergillus fumigatus and Preussia isabellae) isolated from Tanzania’s mangroves	10
Aspergillus terreus camptothecin-sodium alginate/titanium dioxide nanoparticles as a novel nanocomposite with enhanced compatibility and anticancer efficiency in vivo	10
Validation and calibration of a novel GEM biosensor for specific detection of Cd2+, Zn2+, and Pb2+	10
ECM-mimetic, NSAIDs loaded thermo-responsive, immunomodulatory hydrogel for rheumatoid arthritis treatment	10
Ferula latisecta gels for synthesis of zinc/silver binary nanoparticles: antibacterial effects against gram-negative and gram-positive bacteria and physicochemical characteristics	10
Derivation of a novel antimicrobial peptide from the Red Sea Brine Pools modified to enhance its anticancer activity against U2OS cells	10
An engineered hypoxia-response promoter for human umbilical cord-derived mesenchymal stem cell-based therapeutics	10
A magnetic solid phase chemiluminescent immunoassay for quantification of Cystatin C in human serum	10
Saccharification and co-fermentation of lignocellulosic biomass by a cockroach-gut bacterial symbiont and yeast cocktail for bioethanol production	10
Efficient delivery of a large-size Cas9-EGFP vector in porcine fetal fibroblasts using a Lonza 4D-Nucleofector system	10
Establishment of an indirect ELISA antibody detection method based on the stable expression of LSDV P32 protein in CHO-K1 cells	9
Fabrication of apigenin and adenosine-loaded nanoparticles against doxorubicin-induced myocardial infarction by reducing inflammation and oxidative stress	9
Dexamethasone-boosted mesenchymal stem cell secretome: insight into hepatic protection	9
High-performance internal circulation anaerobic granular sludge reactor for cattle slaughterhouse wastewater treatment and simultaneous biogas production	9
Green and environmentally friendly synthesis of silver nanoparticles with antibacterial properties from some medicinal plants	9
An efficient and specific CRISPR-Cas9 genome editing system targeting soybean phytoene desaturase genes	9
Utilizing non-clonal CHO cell derived materials for preclinical studies of complex molecules	8
A novel starch-active lytic polysaccharide monooxygenase discovered with bioinformatics screening and its application in textile desizing	8
Rescue of an enterotropic Newcastle disease virus strain ZM10 from cloned cDNA and stable expressing an inserted foreign gene	8
Enhancing the biotransformation of progesterone to the anticancer compound testololactone by Penicillium chrysogenum Ras3009: kinetic modelling and efficiency maximization	8
Antifungal efficacy of Citrusfusion mediated silver nanoparticles in Candida species	8
Organosolv-derived lipids from hemicellulose and cellulose, and pre-extracted tannins as additives upon hydrothermal liquefaction (HTL) of spruce bark lignins to bio-oil	8
An anti-idiotypic single domain antibody as Cry2Aa toxin mimotope and analysis of its binding region through molecular docking	8
Development of a CHO cell line for stable production of recombinant antibodies against human MMP9	8
Orange peel-mediated synthesis of silver nanoparticles with antioxidant and antitumor activities	7
Dual release of daptomycin and BMP-2 from a composite of β-TCP ceramic and ADA gelatin	7
Integration of ZnO nanorods with silver ions by a facile co-precipitation for antimicrobial, larvicidal, and ovicidal activity	7
Bioconversion of wild Ipomoea pes-caprae and Suaeda fruticosa biomass: a novel application of thermostable xylanase from Neobacillus sedimentimangrovi	7
Exploring the microbial diversity and characterization of cellulase and hemicellulase genes in goat rumen: a metagenomic approach	7
Assessment of the bioactive compounds in gamma irradiated stevia (Stevia rebaudiana Bertoni) leaves	7
Coupling fermentation of glutamic acid and γ-polyglutamic acid and preparation of poly(amino acid) superabsorbent polymers	7
An engineered self-cleavage fusion system for the production of chimaera spider silk proteins	7
Development of a chemically defined medium for Paenibacillus polymyxa by parallel online monitoring of the respiration activity in microtiter plates	7
Synthesis and evaluation of nanosized aluminum MOF encapsulating Umbelliferon: assessing antioxidant, anti-inflammatory, and wound healing potential in an earthworm model	7
Efficient extraction and antioxidant activity of polyphenols from Antrodia cinnamomea	7

Optimization of culture conditions for HBV-specific T cell expansion in vitro from chronically infected patients	7
Comparison of active site mutations at subsite + 2 of Anoxybacillus ayderensis A9 β-glucosidase for hydrolysis of pNPG and polydatin	7
Research on the targeted improvement of the yield of a new VB12-producing strain, Ensifer adhaerens S305, based on genomic and transcriptomic analysis	6
Introduction of loxP sites by electroporation in the mouse genome; a simple approach for conditional allele generation in complex targeting loci	6
rocF affects the production of tetramethylpyrazine in fermented soybeans with Bacillus subtilis BJ3-2	6
Identification, expression, and purification of DNA cytosine 5-methyltransferases with short recognition sequences	6
Effects of solid lipid nanocarrier containing methyl urolithin A by coating folate-bound chitosan and evaluation of its anti-cancer activity	6
Scientific and technological challenges of recombinant egg protein production	6
Enhanced extracellular production of Coprinopsis cinerea laccase Lcc9 in Aspergillus niger by gene expression cassette and bioprocess optimization	6
Induction of antimicrobial, antioxidant metabolites production by co-cultivation of two red-sea-sponge-associated Aspergillus sp. CO2 and Bacillus sp. COBZ21	6
Design, synthesis, and biological evaluation of Osimertinib–Cy7 (OSA-Cy7) conjugate as potential theranostic agent targeting activating EGFR mutations	6
Choosing an appropriate somatic embryogenesis medium of carrot (Daucus carota L.) by data mining technology	6
Influence of low-intensity artificial light on the fatty acid profile of the biotechnologically important culinary mushroom Pleurotus eryngii in vitro	6
Oleic acid based experimental evolution of Bacillus megaterium yielding an enhanced P450 BM3 variant	6
Assessment of biochar filter application in improving chromium stress tolerance and plant physiology in Chinese cabbage (Brassica rapa) under a flow-through water setup	6
Development of a chemiluminescence assay for tissue plasminogen activator inhibitor complex and its applicability to gastric cancer	6
Preparation and characterization of propolis reinforced eggshell membrane/ GelMA composite hydrogel for biomedical applications	6
The transcriptional factor Clr-5 is involved in cellulose degradation through regulation of amino acid metabolism in Neurospora crassa	6
Prediction and optimization of indirect shoot regeneration of Passiflora caerulea using machine learning and optimization algorithms	6
Microarray-based evaluation of selected recombinant timothy grass allergens expressed in E. Coli and N. Benthamiana	5
Uncovering the potential of biofabricated Ananas comosus peel selenium nanoparticles for antibacterial, antibiofilm, suppression of virulence genes (can and LuxS), anticancer, and antioxidant properti	5
Correction: Purification, characterization, and anti-cancer activity of methionine gamma-lyase from a native strain of Pseudomonas mosselii for human cancer treatment	5
MiR-125b-5p ameliorates ox-LDL-induced vascular endothelial cell dysfunction by negatively regulating TNFSF4/TLR4/NF-κB signaling	5
Purification, characterization, and anti-cancer activity of methionine gamma-lyase from a native strain of Pseudomonas mosselii for human cancer treatment	5
Efficient gene delivery into the embryonic chicken brain using neuron-specific promoters and in ovo electroporation	5
Comparison of lipidome profiles in serum from lactating dairy cows supplemented with Acremonium terrestris culture based on UPLC-QTRAP-MS/MS	5
Self-assembling T7 phage syringes with modular genomes for targeted delivery of penicillin against β-lactam-resistant Escherichia coli	5
Unraveling the impact of pH, sodium concentration, and medium osmolality on Vibrio natriegens in batch processes	5
Toxic effects of trace phenol/guanidine isothiocyanate (P/GI) on cells cultured nearby in covered 96-well plates	5
Expansion of targetable sites for the ribonucleoprotein-based CRISPR/Cas9 system in the silkworm Bombyx mori	5
Early osteogenic differentiation of human dental stem cells by gelatin/calcium phosphate- Punica granatum nanocomposite scaffold	5
Effect of different decellularization protocols on reendothelialization with human cells for a perfused renal bioscaffold of the rat	5
Tofu processing wastewater as a low-cost substrate for high activity nattokinase production using Bacillus subtilis	5
Biogenic silver nanoparticles synthesized from Pseudomonas fluorescens-mediated olive cake waste: antimicrobial, larvicidal activity against Culex pipiens and cytotoxicity assessment	5
In vitro evaluation of PLGA loaded hesperidin on colorectal cancer cell lines: an insight into nano delivery system	5
Strain-specific features of Pleurotus ostreatus growth in vitro and some of its biological activities	5
Determination of serum CA724 levels using fluorescence immunochromatography	5
Antibacterial and antibiofilm potentials of vancomycin-loaded niosomal drug delivery system against methicillin-resistant Staphylococcus aureus (MRSA) infections	5
Adsorption of Hg2+/Cr6+ by metal-binding proteins heterologously expressed in Escherichia coli	4
Impacts of loading thymoquinone to gold or silver nanoparticles on the efficacy of anti-tumor treatments in breast cancer with or without chemotherapeutic cisplatin	4
E7 peptide and magnesium oxide-functionalized coaxial fibre membranes enhance the recruitment of bone marrow mesenchymal stem cells and promote bone regeneration	4
Modeling and optimizing in vitro percentage and speed callus induction of carrot via Multilayer Perceptron-Single point discrete GA and radial basis function	4
Statistical optimization of pectinases from thermophilic Aspergillus fumigatus BT-4 employing response surface methodology through submerged fermentation using agricultural wastes	4
Antifungal, toxicological, and colorimetric properties of Origanum vulgare, Moringa oleifera, and Cinnamomum verum essential oils mixture against Egyptian Prince Yusuf Palace deteriorative fungi	4
Green synthesis of silver nanoparticles using cocoon extract of Bombyx mori L.: therapeutic potential in antibacterial, antioxidant, anti-inflammatory, and anti-tumor applications	4
Improving the regeneration rate of deep lethal mutant protoplasts by fusion to promote efficient L-lysine fermentation	4
Online monitoring of the mitochondrial respiration activity and protein formation in the Almost Living Cell-free Expression (ALiCE) system	4
An online soft sensor method for biochemical reaction process based on JS-ISSA-XGBoost	4
Enhanced vitamin B12 production by isolated Bacillus strains with the application of response surface methodology	4
Development of Virus-Like Particles (VLPs) for Hepatitis C Virus genotype 4: a novel approach for vaccine development in Egypt	4
Antimicrobial, antidiabetic, antiviral, and antioxidant activities of fucoidan extracted from the brown seaweed Padina pavonica	4
Antibacterial effect of cerium oxide nanoparticle against Pseudomonas aeruginosa	3
Optimization, characterization and biosafety of carotenoids produced from whey using Micrococcus luteus	3
A cleavable peptide adapter augments the activity of targeted toxins in combination with the glycosidic endosomal escape enhancer SO1861	3
A solution for highly efficient electroporation of primary cytotoxic T lymphocytes	3
Production of rhamnolipid biosurfactants in solid-state fermentation: process optimization and characterization studies	3
Identification and characterization of sugar-regulated promoters in Chaetomium thermophilum	3
Lipid extraction and analysis of microalgae strain pectinodesmus PHM3 for biodiesel production	3
UGT708S6 from Dendrobium catenatum, catalyzes the formation of flavonoid C-glycosides	3
Effects of early geometric confinement on the transcriptomic profile of human cerebral organoids	3
Extract optimization and biological activities of Otidea onotica using Artificial Neural Network-Genetic Algorithm and response surface methodology techniques	3
Correction: Transcriptomic and targeted metabolomic analyses provide insights into the flavonoids biosynthesis in the flowers of Lonicera macranthoides	3
Preparation of an anti-NEK2 monoclonal antibody and its application in liver cancer	3
Comparative evaluation of various DNA extraction methods and analysis of DNA degradation levels in commercially marketed Chestnut rose juices and beverages	3
Nitric oxide mediates positive regulation of Nostoc flagelliforme polysaccharide yield via potential S-nitrosylation of G6PDH and UGDH	3
Analysis of hsa_circ_0136256 as a biomarker for fibrosis in systemic sclerosis	3
Use of immunoinformatics and the simulation approach to identify Helicobacter pylori epitopes to design a multi-epitope subunit vaccine for B- and T-cells	3
Production of SARS-CoV-2 virus-like particles as a vaccine candidate in stable cell lines through inducible E and M protein expression	3
Kinetics of cellulase-free endo xylanase hyper-synthesis by Aspergillus Niger using wheat bran as a potential solid substrate	3
Metabolic studies of Ogataea polymorpha using nine different corn steep liquors	3
Artificial Neural Network - Multi-Objective Genetic Algorithm based optimization for the enhanced pigment accumulation in Synechocystis sp. PCC 6803	3
A study on the processing technology for Rhizoma Coptidis	2
Antibacterial, antibiofilm, and anticancer activity of silver-nanoparticles synthesized from the cell-filtrate of Streptomyces enissocaesilis	2
Screening of Candida spp. in wastewater in Brazil during COVID-19 pandemic: workflow for monitoring fungal pathogens	2
Transcriptomic and targeted metabolomic analyses provide insights into the flavonoids biosynthesis in the flowers of Lonicera macranthoides	2
Anticancer effects of folic acid-functionalized covalent organic framework containing doxorubicin on SW480 colon cancer cells: a promising tool for drug targeted delivery	2
Multiprotein collagen/keratin hydrogel promoted myogenesis and angiogenesis of injured skeletal muscles in a mouse model	2
In vitro assessment of the effect of magnetic fields on efficacy of biosynthesized selenium nanoparticles by Alborzia kermanshahica	2
Recombinant human enamelin produced in Escherichia coli promotes mineralization in vitro	2
Potential protective efficacy of biogenic silver nanoparticles synthesised from earthworm extract in a septic mice model	2

Design of a new lyoprotectant increasing freeze-dried Lactobacillus strain survival to long-term storage	2
Investigation of neomycin biodegradation conditions using ericoid mycorrhizal and white rot fungal species	2
Suppression of mycotoxins production and efficient chelation of heavy metals using natural melanin originated from Aspergillus flavus and Aspergillus carbonarius	2
A simplified Gibson assembly method for site directed mutagenesis by re-use of standard, and entirely complementary, mutagenesis primers	2
A new antifungal compound from Streptomyces diastatochromogenes	2
Chemical and Heat Treatment for Viral Inactivation in Porcine-Derived Gelatin	2
An effective device to enable consistent scratches for in vitro scratch assays	2
αvβ3-targeted sEVs for efficient intracellular delivery of proteins using MFG-E8	2
Development of a chemiluminescent immunoassay based on magnetic solid phase for quantification of homocysteine in human serum	2
Antifungal screening of selenium nanoparticles biosynthesized by microcystin-producing Desmonostoc alborizicum	2
Limonene encapsulated alginate/collagen as antibiofilm drug against Acinetobacter baumannii	2
Design of a novel multi-epitope vaccine against Marburg virus using immunoinformatics studies	2
Lactococcus lactis I7 isolated from traditional Italian cheese making: a biotechnological integrated platform	2
Solubility of lamotrigine in age-specific biorelevant media that simulated the fasted- and fed-conditions of the gastric and intestinal environments in pediatrics and adults: implications for traditio	2
Extract optimization of Ulva lactuca L. and biological activities of optimized extracts	2
Self-limiting fall armyworm: a new approach in development for sustainable crop protection and resistance management	2
Exploring the mechanisms and mode of action of bioactive compounds from marine Streptomyces albidoflavus against breast carcinoma cells	2
Antifungal activity and biocompatibility assessment with molecular docking and dynamic simulations of new pyrazole derivatives	2
Modification of improved-genome editing via oviductal nucleic acids delivery (i-GONAD)-mediated knock-in in rats	2
Characterization of a highly xylose tolerant β-xylosidase isolated from high temperature horse manure compost	2
Metabolic engineering for microbial production of sugar acids	2