OOIR: Observatory of International Research

Papers

(The TQCC of BMC Biotechnology is 8. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2022-08-01 to 2026-08-01.)

Article	Citations
Systematic engineering of Escherichia coli for biosynthesis of 3-hydroxypropionic acid from glucose and malonate	77
Parabacteroides distasonis promotes CXCL9 secretion of tumor-associated macrophages and enhances CD8+T cell activity to trigger anti-tumor immunity against anti-PD-1 treatment in non-small cell lung c	74
A novel single-vector featuring bidirectional promoters for production of bispecific antibody against rabies virus	70
Improvement and prediction of the extraction parameters of lupeol and stigmasterol metabolites of Melia azedarach with response surface methodology	43
Identification of a novel fully human anti-toxic shock syndrome toxin (TSST)-1 single-chain variable fragment antibody averting TSST-1-induced mitogenesis and cytokine secretion	38
3D-printed metamaterial femoral prostheses via scalar field fusion and directional porous structure regulation	38
Efficient removal of Congo Red Dye using a MgAl-LDH/cuttlebone composite adsorbent	36
MGS-HCT enables resistance gene discovery and functional sequence design in forest trees	32
Simplified cloning and isolation of peptides from “sandwiched” SUMO-peptide-intein fusion proteins	32
Enhanced production of Nattokinase by Bacillus aryabhattai RP6 isolated from fermented Brassica oleracea	31
Optimized zymogram protocol from 3D spheroid cultures to study MMP-2 and -9 activities in tumor cells	29
A streamlined and comprehensive protocol for the generation and multi-omic analysis of human monocyte-derived macrophages	29
3D printing of Ceffe-infused scaffolds for tailored nipple-like cartilage development	28
Overexpression of NtGSTU10 gene enhances nicotine content in hybrids by promoting the synthesis and transport metabolism of nicotine	28
N-terminal truncation of the foot-and-mouth disease virus VP0 protein promotes soluble co-expression and particle assembly stability of viral capsid proteins in Escherichia coli	28
Determination of the cytotoxic effects of parasporal proteins in native Bacillus thuringiensis isolates on various cancer cell lines and investigation of the apoptotic, synergistic, and angiogenic pot	27
Yeast surface display-based screening and preliminary characterization of nectin-4-targeting nanobodies for bladder cancer	26
Synergistic effect of zinc oxide-cinnamic acid nanoparticles for wound healing management: in vitro and zebrafish model studies	26
Biocompatibility and potential anticancer activity of gadolinium oxide (Gd2O3) nanoparticles against nasal squamous cell carcinoma	26
Gellan gum/gelatin Schiff-base hydrogel for ocular drug delivery of timolol maleate	25
Biosafety and pharmacokinetic characteristics of polyethylene pyrrolidone modified nano selenium in rats	24
Maximizing lipid accumulation in Tetradesmus obliquus under heavy metal stress for sustainable biodiesel innovation	24
Comparison of vector elements and process conditions in transient and stable suspension HEK293 platforms using SARS-CoV-2 receptor binding domain as a model protein	23
Transgenic Arabidopsis thaliana plants expressing bacterial γ-hexachlorocyclohexane dehydrochlorinase LinA	23
Ligand-dependent corepressor suppresses angiogenesis and macrophage infiltration in gliobl astoma microenvironment via MCP-1 down-regulation	22

Fabrication and characterization of metformin-loaded PLGA/Collagen nanofibers for modulation of macrophage polarization for tissue engineering and regenerative medicine	22
Fusarium verticillioides pigment: production, response surface optimization, gamma irradiation and encapsulation studies	22
Metformin potentiates DSF/Cu-loaded pluronic nanoparticles to improve therapeutic efficacy in triple-negative breast cancer	21
Breaking the barrier of interspecific recalcitrance: a unified and efficient in vitro conservation system for Passiflora germplasm	21
Evaluating the anticancer efficacy of Chara vulgaris ethanolic extract and selenium nanoformulation in Ehrlich carcinoma mice: role of autophagy and apoptosis	20
Rice yellow mottle virus is a suitable amplicon vector for an efficient production of an anti-leishmianiasis vaccine in Nicotiana benthamiana leaves	20
Highly multiplexed targeted sequencing strategy for infectious disease surveillance	20
Electrospun PCL membranes for localized drug delivery and bone regeneration	20
Generation of a recombinant antibody for sensitive detection of Pseudomonas aeruginosa	19
The ESRP1 promoter reporter can function as an in vivo sensor of DNA methyltransferase inhibition	19
Biochemical characterization and insights into the potency of the acidic Aspergillus niger NRC114 purified α-galactosidase in removing raffinose family oligosaccharides from soymilk yogurt	19
A new mRNA structure prediction based approach to identifying improved signal peptides for bone morphogenetic protein 2	19
Chitosan nano-formulation enhances stability and bactericidal activity of the lytic phage HK6	18
Purification and characterization of soluble recombinant Crimean-Congo hemorrhagic fever virus glycoprotein Gc expressed in mammalian 293F cells	18
Soft sensor modeling method for Pichia pastoris fermentation process based on substructure domain transfer learning	18
In vitro and in silico analysis of the fungicidal potential of secondary metabolites from a bacterial endophyte against Botrytis cinerea	18
FOXD1, a hypoxia-related gene, accelerates prostate cancer cell growth by increasing glycolysis under hypoxia conditions	18
Newly designed curcumin-loaded hybrid nanoparticles: a multifunctional strategy for combating oxidative stress, inflammation, and infections to accelerate wound healing and tissue regeneration	17
Central position of histidine in the sequence of designed alternating polarity peptides enhances pH-responsive assembly with DNA	17
In silico screening and in vitro biological evaluation reveal Queuine as a promising MAP4K4 inhibitor for treating pancreatic cancer	17
A critical role for erythropoietin on vagus nerve Schwann cells in intestinal motility	16
Anti-inflammatory potential of aspergillus unguis SP51-EGY: TLR4-dependent effects & chemical diversity via Q-TOF LC-HRMS	16
Isolation and identification of antifungal, antibacterial and nematocide agents from marine bacillus gottheilii MSB1	16
Optimization of nitrogen removal and microbial communities in biofloc systems via carbon-to-nitrogen ratio regulation and Candida utilis	15
Modified langendorff-free method for isolating cardiomyocytes and non-cardiomyocytes from adult mice	15
Gene cloning and molecular characterization of a thermostable chitosanase from Bacillus cereus TY24	15
Molecular and agro-morphological characterization of new barley genotypes in arid environments	14
Protease sensitivity and stress adaptation of bioactive peptide-producing lactic acid bacteria: functional implications for food biopreservation	14
Comparative analysis of horsetail and lavender-derived nanovesicles in wound healing and antioxidant defense	14
A novel allogeneic acellular matrix scaffold for porcine cartilage regeneration	14
Design, optimization of Niosomal gel capability for solubility and bioavailability enhancement of Kynurenic acid after intranasal administration: In Vitro and In Vivo Evaluation	14
Polyurethane nanofibrous scaffolds for wound repair: from electrospinning fabrication to biological performance evaluation	14
Characterization of broad-host-range Salmonella phage GSP006 and its efficacy in controlling Salmonella Pullorum contamination in poultry feed and drinking water	14
Aripiprazole-loaded niosome/chitosan-gold nanoparticles for breast cancer chemo-photo therapy	14
Establishment and in vitro validation of a sheep-specific, low-cost TLR7/8 agonist R848-mediated X/Y sperm separation system	13
Improvements of gamma radiation-induced immunological, hematological, and some biochemical changes in male albino rats by custard apple (Annona squamosa) seed oil extract	13
Derivation of a novel antimicrobial peptide from the Red Sea Brine Pools modified to enhance its anticancer activity against U2OS cells	13
Scaling the manufacture of adipose tissue-derived mesenchymal stromal cells: integrated bioreactor workflows from inoculation to harvest without the need of seed train	13
Interpretable machine learning reveals metabolomic signatures: biomarkers and mechanisms in acute vs chronic angle-closure glaucoma	13
An engineered hypoxia-response promoter for human umbilical cord-derived mesenchymal stem cell-based therapeutics	13
Saccharification and co-fermentation of lignocellulosic biomass by a cockroach-gut bacterial symbiont and yeast cocktail for bioethanol production	13
Unlocking the antioxidant and antibacterial potential of exopolysaccharides produced by endophytic fungi (Aspergillus fumigatus and Preussia isabellae) isolated from Tanzania’s mangroves	12
Anticancer activity of an apigenin-functionalized silica-coated silver oxide nanocomposite against human gastric adenocarcinoma cells (AGS): in vitro and in silico analysis	12
ECM-mimetic, NSAIDs loaded thermo-responsive, immunomodulatory hydrogel for rheumatoid arthritis treatment	12
Enhancing the biotransformation of progesterone to the anticancer compound testololactone by Penicillium chrysogenum Ras3009: kinetic modelling and efficiency maximization	12
Efficient delivery of a large-size Cas9-EGFP vector in porcine fetal fibroblasts using a Lonza 4D-Nucleofector system	12
Ferula latisecta gels for synthesis of zinc/silver binary nanoparticles: antibacterial effects against gram-negative and gram-positive bacteria and physicochemical characteristics	12
Propolis-functionalized acellular fish skin scaffolds as biotechnological platforms for antimicrobial activity and regenerative wound healing	12
Dexamethasone-boosted mesenchymal stem cell secretome: insight into hepatic protection	12
Validation and calibration of a novel GEM biosensor for specific detection of Cd2+, Zn2+, and Pb2+	12

Phyto mediated synthesis of copper oxide nanoparticles with Zingiber officinale: comprehensive bioactivity assessment against bacterial pathogens, cancer, and viruses	12
Seasonal variations in microbial and physicochemical parameters of Nile river water in Giza, Egypt: comparison of IDEXX and conventional methods	12
A magnetic solid phase chemiluminescent immunoassay for quantification of Cystatin C in human serum	12
Green and environmentally friendly synthesis of silver nanoparticles with antibacterial properties from some medicinal plants	11
Mechanistic insights into how cichoriin inhibits P2Y14R to suppress MSU-induced gouty inflammation	11
High-performance internal circulation anaerobic granular sludge reactor for cattle slaughterhouse wastewater treatment and simultaneous biogas production	11
Aspergillus terreus camptothecin-sodium alginate/titanium dioxide nanoparticles as a novel nanocomposite with enhanced compatibility and anticancer efficiency in vivo	11
Fabrication of apigenin and adenosine-loaded nanoparticles against doxorubicin-induced myocardial infarction by reducing inflammation and oxidative stress	11
Establishment of an indirect ELISA antibody detection method based on the stable expression of LSDV P32 protein in CHO-K1 cells	11
Synthesis and evaluation of nanosized aluminum MOF encapsulating Umbelliferon: assessing antioxidant, anti-inflammatory, and wound healing potential in an earthworm model	10
Antifungal efficacy of Citrusfusion mediated silver nanoparticles in Candida species	10
Rescue of an enterotropic Newcastle disease virus strain ZM10 from cloned cDNA and stable expressing an inserted foreign gene	10
Comparative analysis of MinION and MiSeq using 16S rRNA gene amplicon sequencing in human gut microbiome	10
Exploring the microbial diversity and characterization of cellulase and hemicellulase genes in goat rumen: a metagenomic approach	10
An anti-idiotypic single domain antibody as Cry2Aa toxin mimotope and analysis of its binding region through molecular docking	10
Electroporation-mediated site-specific knock-in of a human coding sequence into the bovine ALB locus in embryos	10
Utilizing non-clonal CHO cell derived materials for preclinical studies of complex molecules	10
Sustainable biohydrogen production from banana peels using microbial fermentation	10
A novel starch-active lytic polysaccharide monooxygenase discovered with bioinformatics screening and its application in textile desizing	10
Organosolv-derived lipids from hemicellulose and cellulose, and pre-extracted tannins as additives upon hydrothermal liquefaction (HTL) of spruce bark lignins to bio-oil	10
α-pinene and β-sitosterol co-loaded alginate/gelatin-functionalized niosomes for enhanced antibacterial and anti-biofilm activity against methicillin-resistant Staphylococcus aureus and carbapenem-res	9
Improving xylitol production by semi-rational engineering of xylitol dehydrogenase and optimizing cofactor regeneration in Gluconobacter oxydans	9
Augmentation of antibacterial activity of ciprofloxacin by bisabololoxide A encapsulated in polymeric nanoparticles against uropathogenic Escherichia coli	9
Orange peel-mediated synthesis of silver nanoparticles with antioxidant and antitumor activities	9
A dual pH/ROS-responsive hyaluronan-gated hollow mesoporous silica nanoplatform with CD44-associated cellular interaction improves curcumin retention under serum challenge and attenuates TNF-α–driven	9
Green-synthesized quantum dots from quercus brantii for infected polymicrobial wound healing: mechanisms and biocompatibility	9
Assessment of the bioactive compounds in gamma irradiated stevia (Stevia rebaudiana Bertoni) leaves	9
Coupling fermentation of glutamic acid and γ-polyglutamic acid and preparation of poly(amino acid) superabsorbent polymers	9
Development and validation of a comprehensive in vitro organ model for androgenetic alopecia	9
Quercetin-loaded PCL scaffold suppresses bone metastatic tumors and augments in situ bone formation	9
Biomass and bioethanol production from pretreated mixed fruit peel hydrolysate using Saccharomyces cerevisiae strains at different pH and oxygen conditions	9
Prediction and optimization of indirect shoot regeneration of Passiflora caerulea using machine learning and optimization algorithms	8
Assessment of biochar filter application in improving chromium stress tolerance and plant physiology in Chinese cabbage (Brassica rapa) under a flow-through water setup	8
Enhanced extracellular production of Coprinopsis cinerea laccase Lcc9 in Aspergillus niger by gene expression cassette and bioprocess optimization	8
Statistical optimization of fermentation conditions for enhanced production of Nattokinase from Bacillus subtilis MM26	8
Sustainable utilization of phosphogypsum in agriculture enhanced by mycorrhizal organisms: a new perspective	8
Bioconversion of wild Ipomoea pes-caprae and Suaeda fruticosa biomass: a novel application of thermostable xylanase from Neobacillus sedimentimangrovi	8
Comparison of active site mutations at subsite + 2 of Anoxybacillus ayderensis A9 β-glucosidase for hydrolysis of pNPG and polydatin	8
Scientific and technological challenges of recombinant egg protein production	8
Synthesis and application of exopolysaccharide-selenium nanocomposite produced by Pseudoduganella armeniaca ZMN-3	8
Integration of ZnO nanorods with silver ions by a facile co-precipitation for antimicrobial, larvicidal, and ovicidal activity	8
Integrated whole-genome resequencing and mRNA-miRNA analysis reveals selection signals and regulatory networks of antipyretic effect variation in Morus alba var. multicaulis	8
Effects of solid lipid nanocarrier containing methyl urolithin A by coating folate-bound chitosan and evaluation of its anti-cancer activity	8
Development of a chemically defined medium for Paenibacillus polymyxa by parallel online monitoring of the respiration activity in microtiter plates	8
Qualitative parameters and deterioration kinetics of palm oil, shea butter and their blend use for frying cheese	8
Influence of low-intensity artificial light on the fatty acid profile of the biotechnologically important culinary mushroom Pleurotus eryngii in vitro	8
Dual release of daptomycin and BMP-2 from a composite of β-TCP ceramic and ADA gelatin	8
Optimization of culture conditions for HBV-specific T cell expansion in vitro from chronically infected patients	8
An engineered self-cleavage fusion system for the production of chimaera spider silk proteins	8
The transcriptional factor Clr-5 is involved in cellulose degradation through regulation of amino acid metabolism in Neurospora crassa	8