Engineering in Life Sciences

Article	Citations
Assessing the combined effect of surface topography and substrate rigidity in human bone marrow stem cell cultures	79
Cover Picture: Engineering in Life Sciences 8'23	75
Machine learning‐based automated fungal cell counting under a complicated background with ilastik and ImageJ	69
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Generation and evaluation of input values for computational analysis of transport processes within tissue cultures	42
A Consecutive Genome Engineering Method Reveals a New Phenotype and Regulation of Glucose and Glycerol Utilization in Clostridium Pasteurianum	38
Pitfalls in Early Bioprocess Development Using Shake Flask Cultivations	36
Investigating Ultrafiltration Membranes and Operation Modes for Improved Lentiviral Vector Processing	35
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Improvement of glycine biosynthesis from one‐carbon compounds and ammonia catalyzed by the glycine cleavage system in vitro	19
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Micro flow photochemical synthesis of Ca‐sensitive fluorescent sensor particles	17
Assessment of microbial activity by CO2 production during heating oil storage	16
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Gas supply apparatus using rotational motion of shaking incubator for flask culture of aerobic microorganisms	15
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Online deployment of an O‐PLS model for dielectric spectroscopy‐based inline monitoring of viable cell concentrations in Chinese hamster ovary cell perfusion cultivations	14
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Efficient phenol degradation by laccase immobilized on functional magnetic nanoparticles in fixed bed reactor under high‐gradient magnetic field	13
Flavonoids, gut microbiota, and host lipid metabolism	12
Application of Artificial Neural Network for predicting biomass growth during domestic wastewater treatment through a biological process	12
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A worldwide survey on the use of animal‐derived materials and reagents in scientific experimentation	11
Using the inner membrane of Escherichia coli as a scaffold to anchor enzymes for metabolic flux enhancement	11
Acceleration of antibacterial activity of curcumin loaded biopolymers against methicillin‐resistant Staphylococcus aureus: Synthesis, optimization, and evaluation	11
Cost‐effective purification process development for chimeric hepatitis B core (HBc) virus‐like particles assisted by molecular dynamic simulation	10
Engineered nickel bioaccumulation in Escherichia coli by NikABCDE transporter and metallothionein overexpression	10
Insights into Streptomyces coelicolor A3(2) growth and pigment formation with high‐throughput online monitoring	10
Evaluation of prokaryotic and eukaryotic microbial communities on microplastic‐associated biofilms in marine and freshwater environments	10
A versatile ceramic capillary membrane reactor system for continuous enzyme‐catalyzed hydrolysis	9
A perspective‐driven and technical evaluation of machine learning in bioreactor scale‐up: A case‐study for potential model developments	9
Purification of pluripotent embryonic stem cells using dielectrophoresis and a flow control system	9
Preparation of lignosulfonate‐based nanofiltration membranes with improved water desalination performance	9
Mechanical Microvibration Device Enhancing Immunohistochemistry Efficiency	9
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Synthetic mutualism in engineered E. coli mutant strains as functional basis for microbial production consortia	8
Optimization of a mAb production process with regard to robustness and product quality using quality by design principles	8
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Secretory Production of Heterologous Antimicrobial Peptides in Corynebacterium glutamicum	8
Genomic markers on synthetic genomes	8
The pro‐inflammatory response of macrophages regulated by acid degradation products of poly(lactide‐co‐glycolide) nanoparticles	7
One‐step selective affinity purification and immobilization of His‐tagged enzyme by recyclable magnetic nanoparticles	7
Removal of heavy metals from industrial wastewater using microbial fuel cell	7
3D‐printed autoclavable plant holders to facilitate large‐scale protein production in plants	7
Phenotype analysis of cultivation processes via unsupervised machine learning: Demonstration for Clostridium pasteurianum	6
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Exploitation of the ribosomal protein L10 R98S mutation to enhance recombinant protein production in mammalian cells	6
Evaluation of SDS‐coated iron nanostructure on the gene expression of bio surfactant‐producing genes by Pseudomonas aeruginosa	6
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Rapid exometabolome footprinting combined with multivariate statistics: A powerful tool for bioprocess optimization	6
Design of a synthesis‐friendly hypoxia‐responsive promoter for cell‐based therapeutics	6
Enhanced sequestration of carbon dioxide into calcium carbonate using pressure and a carbonic anhydrase from alkaliphilic Coleofasciculus chthonoplastes	5
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Application of an Escherichia coli triple reporter strain for at‐line monitoring of single‐cell physiology during L‐phenylalanine production	5
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Flexible biofilm monitoring device	5
Effect of metals on the regulation of acidogenic metabolism enhancing biohydrogen and carboxylic acids production from brewery spent grains: Microbial dynamics and biochemical analysis	5
A novel twin‐column continuous chromatography approach for separation and enrichment of monoclonal antibody charge variants	5
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Characterization of an aerated submerged hollow fiber ultrafiltration device for efficient microalgae harvesting	5

Development of a 3D‐printed single‐use separation chamber for use in mRNA‐based vaccine production with magnetic microparticles	5
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Mobile SARS‑CoV‑2 screening facilities for rapid deployment and university‐based diagnostic laboratory	4
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Monitoring the apical growth characteristics of hairy roots using non‐invasive laser speckle contrast imaging	4
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An account on the innovative path of cooperation with Prof. Zhiguo Su in bioseparation	4
An optimized live bacterial delivery vehicle safely and efficaciously delivers bacterially transcribed therapeutic nucleic acids	4
Stable overexpression of native and artificial miRNAs for the production of differentially fucosylated antibodies in CHO cells	4
Cell‐free synthesis of silver nanoparticles in spent media of different Aspergillus species	4
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Salting‐out extraction of recombinant κ‐carrageenase and phage T7 released fromEscherichia colicells	4
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R‐based method for quantitative analysis of biofilm thickness by using confocal laser scanning microscopy	4
Implementation of ubiquitous chromatin opening elements as artificial integration sites for CRISPR/Cas9‐mediated knock‐in in mammalian cells	4
Invitro biocompatibility evaluation of a heat‐resistant 3D printing material for use in customized cell culture devices	3
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In situ Product Recovery of Microbially Synthesized Ethyl Acetate from the Exhaust Gas of a Bioreactor by Membrane Technology	3
Interrogation of the perturbed gut microbiota in gouty arthritis patients through in silico metabolic modeling	3
Evaluation of Yeast Alcohol Acetyltransferases for Ethyl Acetate Production in Clostridium ljungdahlii	3
Preparation of a pH‐responsive chitosan‐montmorillonite‐nitrogen‐doped carbon quantum dots nanocarrier for attenuating doxorubicin limitations in cancer therapy	2
Modeling multiphase fluid flow, mass transfer, and chemical reactions in bioreactors using large‐eddy simulation	2
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Developing a surface acoustic wave‐induced microfluidic cell lysis device for point‐of‐care DNA amplification	2
Downstream bioprocessing of human pluripotent stem cell‐derived therapeutics	2
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Magnetizing Biotech–Advances in (In Vivo) Magnetic Enzyme Immobilization	2
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Effects and interactions of metal oxides in microparticle‐enhanced cultivation of filamentous microorganisms	2
Microbial Squalene: A Sustainable Alternative for the Cosmetics and Pharmaceutical Industry – A Review	2
Fabrication of Au/Fe3O4/RGO based aptasensor for measurement of miRNA‐128, a biomarker for acute lymphoblastic leukemia (ALL)	2
The cultivation conditions affect the aggregation and functionality of β‐cell lines alone and in coculture with mesenchymal stromal/stem cells	2
Combination of Two‐Stage Continuous Feeding and Optimized Synthetic Medium Increases Lipid Production in Lipomyces starkeyi	2
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Ensemble‐based adaptive soft sensor for fault‐tolerant biomass monitoring	1
Evaluation of restricted access media for the purification of cell culture‐derived Orf viruses	1
Bioprocess development for endospore production by Bacillus coagulans using an optimized chemically defined medium	1
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Simultaneous solid–liquid separation and primary purification of clavulanic acid from fermentation broth of Streptomyces clavuligerus using salting‐out extraction system	1
Study on the development and integration of 3D‐printed optics in small‐scale productions of single‐use cultivation vessels	1
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Assessment of extraction options for a next‐generation biofuel: Recovery of bio‐isobutanol from aqueous solutions	1
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Downstream processing of bioproducts	1
Optimization of bioprocesses with Brewers’ spent grain and Cellulomonas uda	1
Agglomeration behaviour of magnetic microparticles during separation and recycling processes in mRNA purification	1
Rapid in‐situ aerobic biodegradation of high salt and oily food waste employing constructed synthetic microbiome	1
Comparison of various classification techniques for supervision of milk processing	1
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Biocompatible scaffolds based on collagen and oxidized dextran for endothelial cell survival and function in tissue engineering	1
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Realization of user‐friendly bioanalytical tools to quantify and monitor critical components in bio‐industrial processes through conceptual design	1
Design of cell expansion processes for adherent‐growing cells with mDoE‐workflow	1
Real‐time droplet size analysis using laser micrometer as a process analytical technology tool for continuous dripping process	0
Citrate Supplementation Modulates Medium Viscosity and Poly‐γ‐Glutamic Acid Synthesis by Engineered B. subtilis 168	0
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Aptasensors versus immunosensors—Which will prevail?	0
Special issue for Thomas Scheper's retirement	0
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Applying intensified design of experiments to mammalian cell culture processes	0
Employing QbD strategies to assess the impact of cell viability and density on the primary recovery of monoclonal antibodies	0
Coupling the fermentation and membrane separation process for polyamides monomer cadaverine production from feedstock lysine	0
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A review of sustainable biodiesel production using biomass derived heterogeneous catalysts	0
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Yeast autonomously replicating sequence (ARS): Identification, function, and modification	0
Stability of a Mutualistic Escherichia coli Co‐Culture During Violacein Production Depends on the Kind of Carbon Source	0

Gut microbiota and childhood malnutrition: Understanding the link and exploring therapeutic interventions	0
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Novel application of anti‐human Fc nanobody for screening high‐producing CHO cells for monoclonal antibody	0
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Membrane inlet—ion mobility spectrometry with automatic spectra evaluation as online monitoring tool for the process control of microalgae cultivation	0
Impact of lipopolysaccharides on cultivation and recombinant protein expression in human embryonal kidney (HEK‐293) cells	0
Editing Streptomyces genome using target AID system fused with UGI‐degradation tag	0
Development of purification process for dual‐function recombinant human heavy‐chain ferritin by the investigation of genetic modification impact on conformation	0
Editorial for the special issue ‘cell‐to‐cell’ and ‘cell‐to‐bioreactor’ interactions	0
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Endospore production of Bacillus spp. for industrial use	0
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Identification and characterization of inulinases by bioinformatics analysis of bacterial glycoside hydrolases family 32 (GH32)	0
Fungal Enzymes for Saccharification of Gamma‐Valerolactone‐Pretreated White Birch Wood: Optimization of the Production of Talaromyces amestolkiae Cellulolytic Cocktail	0
Stress‐induced increase of monoclonal antibody production in CHO cells	0
Microbiome research for advancing engineering in life science	0
Genetic modules for α‐factor pheromone controlled growth regulation of Saccharomyces cerevisiae	0
Novel bioreactor internals for the cultivation of spore‐forming fungi in pellet form	0
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A Cofactor Regeneration System for 2‐Aminobutyric Acid Production Based on Combined Cross‐Linked Enzyme Aggregates: Utilizing His‐Tagged Enzymes With Low‐Concentration Calcium Ions as Precipitant	0
Site‐directed mutagenesis improves the practical application of L‐glutamic acid decarboxylase inEscherichia coli	0
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Efficient co‐production of propionic acid and succinic acid by Propionibacterium acidipropionici using membrane separation coupled technology	0
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Recent advances in microbial capture of hydrogen sulfide from sour gas via sulfur‐oxidizing bacteria	0
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Evaluating scaling of capillary photo‐biofilm reactors for high cell density cultivation of mixed trophies artificial microbial consortia	0
Digitalization concepts in academic bioprocess development	0
Artificial microbial consortia for bioproduction processes	0
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Novel synthetic co‐culture of Acetobacterium woodii and Clostridium drakei using CO2 and in situ generated H2 for the production of caproic acid via lactic acid	0
Multi‐objective Bayesian algorithm automatically discovers low‐cost high‐growth serum‐free media for cellular agriculture application	0
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Optimizations of Placenta Extracellular Matrix‐Loaded Silk Fibroin/Alginate 3D‐Printed Scaffolds Structurally and Functionally for Bone Tissue Engineering	0
Regulation on C2‐C8 carboxylic acid biosynthesis from anaerobic CO2 fermentation	0
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Strategies to achieve high productivity, high conversion, and high yield in yeast fermentation of algal biomass hydrolysate	0
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Study on dynamic changes of microbial community and lignocellulose transformation mechanism during green waste composting	0
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Ultra pH‐sensitive detection of total and free prostate‐specific antigen using electrochemical aptasensor based on reduced graphene oxide/gold nanoparticles emphasis on TiO2/carbon quantum	0
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CRISPRi enables fast growth followed by stable aerobic pyruvate formation in Escherichia coli without auxotrophy	0
Enzyme immobilization in hydrogels: A perfect liaison for efficient and sustainable biocatalysis	0
Meet Our Editorial Board—Engineering in Life Sciences. An Interview with Antonina “Tonya” Lavrentieva, Leibniz Universität Hannover, Institut für Technische Chemie, Hannover, Germany	0
Integration of a perfusion reactor and continuous precipitation in an entirely membrane‐based process for antibody capture	0
Real‐Time VCC Monitoring and Forecasting in HEK‐Cell‐Based rAAV Vector Production Using Capacitance Spectroscopy	0
Enrichment of the rebaudioside A concentration in Stevia rebaudiana extract with cyclodextrin glycosyltransferase from Bacillus licheniformis DSM 13	0
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Photobioreactors for cultivation and synthesis: Specifications, challenges, and perspectives	0
Risk control of host cell proteins in one therapeutic antibody produced by concentrated fed‐batch (CFB) mode	0
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Vascular implants – new aspects for in situ tissue engineering	0
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Mini Bubble Columns for Miniaturizing Scale‐Down	0
Advances in automated real‐time flow cytometry for monitoring of bioreactor processes	0
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Influence of pH value and salts on the adsorption of lysozyme in mixed‐mode chromatography	0
Biological recovery of phosphorus (BioP‐Rec) from wastewater streams using brewer's yeast on pilot‐scale	0
Current trends in medium‐chain‐length polyhydroxyalkanoates: Microbial production, purification, and characterization	0
From single‐cell cloning to high‐yield influenza virus production – implementing advanced technologies in vaccine process development	0
Improving Downstream Process Related Manufacturability Based on Protein Engineering—A Feasibility Study	0
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Synthesis and Evaluation of PCL/Chitosan/CQD‐Fe Magnetic Nanocomposite for Wound Healing: Emphasis on Gene Expression	0
Succinic Acid Production With Actinobacillus succinogenes –Influence of an Electric Potential on the Intercellular NADH/NAD+ Balance	0
A novel approach for enumeration of extracellular vesicles from crude and purified cell culture samples	0
A novel downstream process for highly pure 1,3‐propanediol from an efficient fed‐batch fermentation of raw glycerol by Clostridium pasteurianum	0
Robot‐based 6D bioprinting for soft tissue biomedical applications	0
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Streamlining of a synthetic co‐culture towards an individually controllable one‐pot process for polyhydroxyalkanoate production from light and CO2	0
Facilitated endospore detection for Bacillus spp. through automated algorithm‐based image processing	0
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FAIR research data management as community approach in bioengineering	0
Enrichment of homoacetogens converting H2/CO2 into acids and ethanol and simultaneous methane production	0
Improving carotenoid production in recombinant yeast, Saccharomyces cerevisiae, using ultrasound‐irradiated two‐phase extractive fermentation	0
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Overproduction and Characterization of Recombinant Soluble Trypanosoma brucei Phospholipase A2	0