OOIR: Observatory of International Research

Papers

(The median citation count of Protein Engineering Design & Selection is 3. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-11-01 to 2025-11-01.)

Article	Citations
Yeast surface display-based identification of ACE2 mutations that modulate SARS-CoV-2 spike binding across multiple mammalian species	59
Enhancing the Thermostability and solubility of a single-domain catalytic antibody	31
Improving plastic degrading enzymes via directed evolution	25
Comprehensive mapping of SARS-CoV-2 peptide epitopes for development of a highly sensitive serological test for total and neutralizing antibodies	25
Improving the production and stability of nanobodies	22
Cyan fluorescent proteins derived from mNeonGreen	22
Yeast biopanning against site-specific phosphorylations in tau	13
Dissection of the MeCP2 repressor protein enables CRISPR platform optimization via localization engineering	12
Enzyme design pioneer Steve Mayo: I was trying to capture the fundamental physics of the problem as a way to elucidate mechanisms	11
Design of functional intrinsically disordered proteins	10
Engineering of a lysosomal-targeted GAA enzyme	9
Engineering enzyme activity using an expanded amino acid alphabet	9
Sequence-developability mapping of affibody and fibronectin paratopes via library-scale variant characterization	9
Correction to: Protease-stable DARPins as promising oral therapeutics	8
Antibody-mediated delivery of CRISPR-Cas9 ribonucleoproteins in human cells	8
abYpap: improvements to the prediction of antibody VH/VL packing using gradient boosted regression	7
Analysis of conformational stability of interacting residues in protein binding interfaces	7
Engineering of a phosphotriesterase with improved stability and enhanced activity for detoxification of the pesticide metabolite malaoxon	7
Modifying pH-sensitive PCSK9/LDLR interactions as a strategy to enhance hepatic cell uptake of low-density lipoprotein cholesterol (LDL-C)	7
Effect of alanine versus serine at position 88 of human transthyretin mutants on the protein stability	7
An easy-to-use high-throughput selection system for the discovery of recombinant protein binders from alternative scaffold libraries	6
Structure-based engineering of minimal proline dehydrogenase domains for inhibitor discovery	6
An engineered NKp46 antibody for construction of multi-specific NK cell engagers	6
Physics-based approach to extend a de novo TIM barrel with rationally designed helix-loop-helix motifs	6
Sequence-activity mapping via depletion reveals striking mutational tolerance and elucidates functional motifs in Tur1a antimicrobial peptide	6

Linker minimization and characterization of Fc-fused interleukin-17A for increased in vivo half-life	5
Design of a ligand-dependent fluorescent biosensor, based on an engineered lipocalin (anticalin), for the sensitive detection of the Alzheimer β -amyloid	5
CDRxAbs: antibody small-molecule conjugates with computationally designed target-binding synergy	5
A protein engineering approach toward understanding FKBP51 conformational dynamics and mechanisms of ligand binding	5
Growing ecosystem of deep learning methods for modeling protein–protein interactions	4
Stabilization of the SARS-CoV-2 receptor binding domain by protein core redesign and deep mutational scanning	4
Optimized single-cell gates for yeast display screening	4
Strategies for enriching and characterizing proteins with inhibitory properties on the yeast surface	4
Tuning ProteinMPNN to reduce protein visibility via MHC Class I through direct preference optimization	3
TIMED-Design: flexible and accessible protein sequence design with convolutional neural networks	3
Enhancing the activity of a monomeric alcohol dehydrogenase for site-specific applications by site-directed mutagenesis	3
Engineered FHA domains can bind to a variety of Phosphothreonine-containing peptides	3
Reducing substrate inhibition of malate dehydrogenase from Geobacillus stearothermophilus by C-terminal truncation	3
Contributions from ClpS surface residues in modulating N-terminal peptide binding and their implications for NAAB development	3