OOIR: Observatory of International Research

Papers

(The median citation count of Protein Engineering Design & Selection is 2. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2022-05-01 to 2026-05-01.)

Article	Citations
Enhancing the Thermostability and solubility of a single-domain catalytic antibody	70
Improving plastic degrading enzymes via directed evolution	40
Rational design of selective bispecific EPO-R/CD131 agonists	35
Improving the production and stability of nanobodies	29
Yeast biopanning against site-specific phosphorylations in tau	25
Dissection of the MeCP2 repressor protein enables CRISPR platform optimization via localization engineering	21
Enzyme design pioneer Steve Mayo: I was trying to capture the fundamental physics of the problem as a way to elucidate mechanisms	15
Design of functional intrinsically disordered proteins	14
Experimental analyses of RFdiffusion designed Miniproteins for binding to SARS-CoV-2 nucleocapsid protein	11
Engineering enzyme activity using an expanded amino acid alphabet	10
A his-to-Gln mutation in catechol 2,3-dioxygenase catalyzes 2,3-dihydroxybenzoic acid cleavage by an intradiol pathway	10
Sequence-developability mapping of affibody and fibronectin paratopes via library-scale variant characterization	9
Integrating machine learning tools in protein design: a case of MHETase engineering for PET biodeconstruction	9
Engineering of a lysosomal-targeted GAA enzyme	8
Analysis of conformational stability of interacting residues in protein binding interfaces	8
Usage of a split CH2 domain as building block for new antibody formats	8
abYpap: improvements to the prediction of antibody VH/VL packing using gradient boosted regression	8
Effect of alanine versus serine at position 88 of human transthyretin mutants on the protein stability	7
Engineering of a phosphotriesterase with improved stability and enhanced activity for detoxification of the pesticide metabolite malaoxon	7
Physics-based approach to extend a de novo TIM barrel with rationally designed helix-loop-helix motifs	6
An easy-to-use high-throughput selection system for the discovery of recombinant protein binders from alternative scaffold libraries	6
Rhizavidin engineered for eukaryotic expression	5
Sequence-activity mapping via depletion reveals striking mutational tolerance and elucidates functional motifs in Tur1a antimicrobial peptide	5
An engineered NKp46 antibody for construction of multi-specific NK cell engagers	4
Design of a ligand-dependent fluorescent biosensor, based on an engineered lipocalin (anticalin), for the sensitive detection of the Alzheimer β -amyloid	3

Linker minimization and characterization of Fc-fused interleukin-17A for increased in vivo half-life	3
Rapid isolation and characterization of high-affinity single-domain antibodies for broad-Spectrum detection of SARS-CoV-2 variants	3
CDRxAbs: antibody small-molecule conjugates with computationally designed target-binding synergy	3
Growing ecosystem of deep learning methods for modeling protein–protein interactions	3
A protein engineering approach toward understanding FKBP51 conformational dynamics and mechanisms of ligand binding	3
Strategies for enriching and characterizing proteins with inhibitory properties on the yeast surface	3
Optimized single-cell gates for yeast display screening	3
Enhancing the activity of a monomeric alcohol dehydrogenase for site-specific applications by site-directed mutagenesis	2
TIMED-Design: flexible and accessible protein sequence design with convolutional neural networks	2
Tuning ProteinMPNN to reduce protein visibility via MHC Class I through direct preference optimization	2
Contributions from ClpS surface residues in modulating N-terminal peptide binding and their implications for NAAB development	2
Bind, catalyze, and quantify: a modern protein and enzyme engineering toolbox of genetically encoded non-canonical amino acids	2