OOIR: Observatory of International Research

Papers

(The median citation count of Protein Engineering Design & Selection is 2. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2022-08-01 to 2026-08-01.)

Article	Citations
A Tethered Yeast Surface Display System Facilitates Enriching Epitope-Specific Binding Interactions	80
Enhancing the Thermostability and solubility of a single-domain catalytic antibody	47
Improving plastic degrading enzymes via directed evolution	42
Rational design of selective bispecific EPO-R/CD131 agonists	29
Yeast biopanning against site-specific phosphorylations in tau	26
Improving the production and stability of nanobodies	26
Dissection of the MeCP2 repressor protein enables CRISPR platform optimization via localization engineering	17
Mapping functional dynamics hotspots for protein engineering with NMR peak intensity analysis	15
Enzyme design pioneer Steve Mayo: I was trying to capture the fundamental physics of the problem as a way to elucidate mechanisms	15
Design of functional intrinsically disordered proteins	14
Experimental analyses of RFdiffusion designed Miniproteins for binding to SARS-CoV-2 nucleocapsid protein	12
A his-to-Gln mutation in catechol 2,3-dioxygenase catalyzes 2,3-dihydroxybenzoic acid cleavage by an intradiol pathway	11
Engineering enzyme activity using an expanded amino acid alphabet	10
Integrating machine learning tools in protein design: a case of MHETase engineering for PET biodeconstruction	10
Usage of a split CH2 domain as building block for new antibody formats	9
Sequence-developability mapping of affibody and fibronectin paratopes via library-scale variant characterization	9
Free energy–guided Q302P mutation in GH1 β -glucosidase from Aspergillus fumigatus improves enzyme functio	8
Engineering of a lysosomal-targeted GAA enzyme	8
Analysis of conformational stability of interacting residues in protein binding interfaces	7
abYpap: improvements to the prediction of antibody VH/VL packing using gradient boosted regression	6
Combining bacterial display and protein language models to engineer a CD69-binding affibody for molecular imaging of immune activation	6
Rhizavidin engineered for eukaryotic expression	5
Engineering of a phosphotriesterase with improved stability and enhanced activity for detoxification of the pesticide metabolite malaoxon	5
Physics-based approach to extend a de novo TIM barrel with rationally designed helix-loop-helix motifs	5
Effect of alanine versus serine at position 88 of human transthyretin mutants on the protein stability	5

An easy-to-use high-throughput selection system for the discovery of recombinant protein binders from alternative scaffold libraries	5
An engineered NKp46 antibody for construction of multi-specific NK cell engagers	4
Sequence-activity mapping via depletion reveals striking mutational tolerance and elucidates functional motifs in Tur1a antimicrobial peptide	4
Design of a ligand-dependent fluorescent biosensor, based on an engineered lipocalin (anticalin), for the sensitive detection of the Alzheimer β -amyloid	3
Protein engineering: Status report	3
Linker minimization and characterization of Fc-fused interleukin-17A for increased in vivo half-life	3
A protein engineering approach toward understanding FKBP51 conformational dynamics and mechanisms of ligand binding	3
Strategies for enriching and characterizing proteins with inhibitory properties on the yeast surface	2
Correction to: Engineered antibody Fc variant with selectively enhanced FcγRIIb binding over both FcγRIIaR131 and FcγRIIaH131	2
Rapid isolation and characterization of high-affinity single-domain antibodies for broad-Spectrum detection of SARS-CoV-2 variants	2
CDRxAbs: antibody small-molecule conjugates with computationally designed target-binding synergy	2
Engineering of tandem bispecific IL-7 receptor agonist antibody promoting selective T cell expansion	2
Growing ecosystem of deep learning methods for modeling protein–protein interactions	2
A photo-enhanced oxidative coupling for site-specific protein Labeling via noncanonical amino acid incorporation	2
Bind, catalyze, and quantify: a modern protein and enzyme engineering toolbox of genetically encoded non-canonical amino acids	2
Optimized single-cell gates for yeast display screening	2