OOIR: Observatory of International Research

Papers

(The TQCC of Protein Engineering Design & Selection is 7. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2022-01-01 to 2026-01-01.)

Article	Citations
Enhancing the Thermostability and solubility of a single-domain catalytic antibody	63
Yeast surface display-based identification of ACE2 mutations that modulate SARS-CoV-2 spike binding across multiple mammalian species	37
Improving plastic degrading enzymes via directed evolution	29
Improving the production and stability of nanobodies	27
Comprehensive mapping of SARS-CoV-2 peptide epitopes for development of a highly sensitive serological test for total and neutralizing antibodies	27
Cyan fluorescent proteins derived from mNeonGreen	22
Rational design of selective bispecific EPO-R/CD131 agonists	15
Yeast biopanning against site-specific phosphorylations in tau	13
Dissection of the MeCP2 repressor protein enables CRISPR platform optimization via localization engineering	12
Enzyme design pioneer Steve Mayo: I was trying to capture the fundamental physics of the problem as a way to elucidate mechanisms	11
A his-to-Gln mutation in catechol 2,3-dioxygenase catalyzes 2,3-dihydroxybenzoic acid cleavage by an intradiol pathway	10
Design of functional intrinsically disordered proteins	10
Engineering enzyme activity using an expanded amino acid alphabet	9
Engineering of a lysosomal-targeted GAA enzyme	9
Antibody-mediated delivery of CRISPR-Cas9 ribonucleoproteins in human cells	8
Sequence-developability mapping of affibody and fibronectin paratopes via library-scale variant characterization	8
Analysis of conformational stability of interacting residues in protein binding interfaces	7
Effect of alanine versus serine at position 88 of human transthyretin mutants on the protein stability	7
Correction to: Protease-stable DARPins as promising oral therapeutics	7
abYpap: improvements to the prediction of antibody VH/VL packing using gradient boosted regression	7
An easy-to-use high-throughput selection system for the discovery of recombinant protein binders from alternative scaffold libraries	7
Modifying pH-sensitive PCSK9/LDLR interactions as a strategy to enhance hepatic cell uptake of low-density lipoprotein cholesterol (LDL-C)	7
Engineering of a phosphotriesterase with improved stability and enhanced activity for detoxification of the pesticide metabolite malaoxon	7
Physics-based approach to extend a de novo TIM barrel with rationally designed helix-loop-helix motifs	7