OOIR: Observatory of International Research

Papers

(The TQCC of Protein Engineering Design & Selection is 6. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-09-01 to 2025-09-01.)

Article	Citations
Improving plastic degrading enzymes via directed evolution	47
Yeast surface display-based identification of ACE2 mutations that modulate SARS-CoV-2 spike binding across multiple mammalian species	29
Enhancing the Thermostability and solubility of a single-domain catalytic antibody	24
Comprehensive mapping of SARS-CoV-2 peptide epitopes for development of a highly sensitive serological test for total and neutralizing antibodies	21
Cyan fluorescent proteins derived from mNeonGreen	20
Dissection of the MeCP2 repressor protein enables CRISPR platform optimization via localization engineering	18
Yeast biopanning against site-specific phosphorylations in tau	12
Design of functional intrinsically disordered proteins	11
Enzyme design pioneer Steve Mayo: I was trying to capture the fundamental physics of the problem as a way to elucidate mechanisms	10
Engineering of a lysosomal-targeted GAA enzyme	9
Engineering enzyme activity using an expanded amino acid alphabet	9
Sequence-developability mapping of affibody and fibronectin paratopes via library-scale variant characterization	8
Modifying pH-sensitive PCSK9/LDLR interactions as a strategy to enhance hepatic cell uptake of low-density lipoprotein cholesterol (LDL-C)	7
Correction to: Protease-stable DARPins as promising oral therapeutics	7
Analysis of conformational stability of interacting residues in protein binding interfaces	7
Antibody-mediated delivery of CRISPR-Cas9 ribonucleoproteins in human cells	7
Physics-based approach to extend a de novo TIM barrel with rationally designed helix-loop-helix motifs	6
Sequence-activity mapping via depletion reveals striking mutational tolerance and elucidates functional motifs in Tur1a antimicrobial peptide	6
Effect of alanine versus serine at position 88 of human transthyretin mutants on the protein stability	6
abYpap: improvements to the prediction of antibody VH/VL packing using gradient boosted regression	6
An easy-to-use high-throughput selection system for the discovery of recombinant protein binders from alternative scaffold libraries	6
Engineering of a phosphotriesterase with improved stability and enhanced activity for detoxification of the pesticide metabolite malaoxon	6
Structure-based engineering of minimal proline dehydrogenase domains for inhibitor discovery	6