Plant Methods

Papers
(The median citation count of Plant Methods is 3. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-04-01 to 2025-04-01.)
ArticleCitations
Protocol: a simple method for biosensor visualization of bacterial quorum sensing and quorum quenching interaction on Medicago roots102
Estimation of chlorophyll content in rice canopy leaves using 3D radiative transfer modeling and unmanned aerial hyperspectral images99
An efficient screening system of disease-resistant genes from wild apple, Malus sieversii in response to Valsa mali pathogenic fungus95
The influence of water content on the longitudinal modulus of elasticity of maize stalk pith and rind tissues71
Soybean leaf estimation based on RGB images and machine learning methods65
Estimation of cotton canopy parameters based on unmanned aerial vehicle (UAV) oblique photography61
Characterizing reference genes for high-fidelity gene expression analysis under different abiotic stresses and elicitor treatments in fenugreek leaves57
A non-destructive approach for measuring rice panicle-level photosynthetic responses using 3D-image reconstruction57
Cryopreservation of sessile oak (Quercus petraea (Matt.) Liebl.) plumules using aluminium cryo-plates: influence of cryoprotection and drying55
A novel selection model based on multivariate methods and arbitrary genetic parameters: a case study on tomato families51
Optimizing PGRs for in vitro shoot proliferation of pomegranate with bayesian-tuned ensemble stacking regression and NSGA-II: a comparative evaluation of machine learning models50
Methods to optimize optical sensing of biotic plant stress – combined effects of hyperspectral imaging at night and spatial binning49
Karst vegetation coverage detection using UAV multispectral vegetation indices and machine learning algorithm46
An automated method for stem diameter measurement based on laser module and deep learning46
A novel visual marker to distinguish haploids from doubled haploids in rice (Oryza sativa, L) at early growth stages45
Determination of phosphorus compounds in plant tissues: from colourimetry to advanced instrumental analytical chemistry43
Axial variation in flexural stiffness of plant stem segments: measurement methods and the influence of measurement uncertainty42
Tensor methods in data analysis of chromatography/mass spectroscopy-based plant metabolomics41
Apnet: Lightweight network for apricot tree disease and pest detection in real-world complex backgrounds40
Accelerating eucalypt clone selection pipeline via cloned progeny trials and molecular data39
Advanced screening methods for assessing motility and hatching in plant-parasitic nematodes38
Application of machine learning algorithms and feature selection in rapeseed (Brassica napus L.) breeding for seed yield37
Hyperspectral imaging-based classification of rice leaf blast severity over multiple growth stages35
Genetic transformation of the oilseed crop camelina using immature zygotic embryos35
Corn360: a method for quantification of corn kernels34
Versatile DNA extraction from diverse plant taxa using ionic liquids and magnetic ionic liquids: a methodological breakthrough for enhanced sample utility32
Computer vision-based wood identification and its expansion and contribution potentials in wood science: A review31
Rapid identification of mutations caused by fast neutron bombardment in Medicago truncatula31
Culture types and period impact gametophyte morphogenesis and sporophyte formation of eastern bracken29
A systematic high-throughput phenotyping assay for sugarcane stalk quality characterization by near-infrared spectroscopy29
Electrical impedance spectroscopy (EIS) in plant roots research: a review29
Real-time monitoring of Ralstonia solanacearum infection progress in tomato and Arabidopsis using bioluminescence imaging technology28
A CNN model for early detection of pepper Phytophthora blight using multispectral imaging, integrating spectral and textural information28
Terrestrial laser scanning and low magnetic field digitization yield similar architectural coarse root traits for 32-year-old Pinus ponderosa trees28
Combining novel feature selection strategy and hyperspectral vegetation indices to predict crop yield27
Ripening dynamics revisited: an automated method to track the development of asynchronous berries on time-lapse images27
Can allele-specific loop-mediated isothermal amplification be used for rapid detection of target-site herbicide resistance in Lolium spp.?27
Study on canopy extraction method for narrowband spectral images based on superpixel color gradation skewness distribution features27
HairNet2: deep learning to quantify cotton leaf hairiness, a complex genetic and environmental trait27
Orchid2024: A cultivar-level dataset and methodology for fine-grained classification of Chinese Cymbidium Orchids27
Applying spectral fractal dimension index to predict the SPAD value of rice leaves under bacterial blight disease stress27
High-throughput phenotyping for non-destructive estimation of soybean fresh biomass using a machine learning model and temporal UAV data26
Exploring the potential of microscopic hyperspectral, Raman, and LIBS for nondestructive quality assessment of diverse rice samples26
Species-specific basecallers improve actual accuracy of nanopore sequencing in plants25
Correction: Maximizing efficiency in sunflower breeding through historical data optimization25
Correction: Revisiting superiority and stability metrics of cultivar performances using genomic data: derivations of new estimators25
A highly efficient protocol for isolation of protoplast from China, Assam and Cambod types of tea plants [Camellia sinensis (L.) O. Kuntze]24
Publisher Correction: New improvements in grapevine genome editing: high efficiency biallelic homozygous knock-out from regenerated plantlets by using an optimized zCas9i24
The isolation strategy and chemical analysis of oil cells from Asari Radix et Rhizoma23
Correction: YOLO POD: a fast and accurate multi-task model for dense Soybean Pod counting23
A handheld device for measuring the diameter at breast height of individual trees using laser ranging and deep-learning based image recognition23
Simple and efficient isolation of plant genomic DNA using magnetic ionic liquids22
Using a thermal gradient table to study plant temperature signalling and response across a temperature spectrum22
Temporal resolution trumps spectral resolution in UAV-based monitoring of cereal senescence dynamics22
Methodology: non-invasive monitoring system based on standing wave ratio for detecting water content variations in plants22
Combining Fourier-transform infrared spectroscopy and multivariate analysis for chemotyping of cell wall composition in Mungbean (Vigna radiata (L.) Wizcek)22
In vivo characterisation of field pea stem wall thickness using optical coherence tomography21
Systematic investigation and validation of peanut genetic transformation via the pollen tube injection method21
Classification of multi-year and multi-variety pumpkin seeds using hyperspectral imaging technology and three-dimensional convolutional neural network20
A novel method for extraction of high purity and high production Phytophthora sojae oospores20
De novo assembly provides new insights into the evolution of Elaeagnus angustifolia L.20
Highly efficient Agrobacterium rhizogenes-mediated transformation for functional analysis in woodland strawberry20
A comprehensive review of in planta stable transformation strategies20
Small-scale sequencing enables quality assessment of Ribo-Seq data: an example from Arabidopsis cell culture19
A quantitative method to measure geranylgeranyl diphosphate (GGPP) and geranylgeranyl monophosphate (GGP) in tomato (Solanum lycopersicum) fruit19
Identification of the initial water-site and movement in Gleditsia sinensis seeds and its relation to seed coat structure19
Detection of Cucumber green mottle mosaic virus in low-concentration virus-infected seeds by improved one-step pre-amplification RT-qPCR19
Protocol: analytical methods for visualizing the indolic precursor network leading to auxin biosynthesis19
Automatic freezing-tolerant rapeseed material recognition using UAV images and deep learning18
High-throughput phenotyping allows the selection of soybean genotypes for earliness and high grain yield18
Stability investigation of air-dried olive ribo nucleic acids for metavirome studies18
Improved genomic prediction using machine learning with Variational Bayesian sparsity18
Characterizing the effects of different chemicals on stem bending of cut snapdragon flower18
AprGPD: the apricot genomic and phenotypic database18
Prediction accuracy and repeatability of UAV based biomass estimation in wheat variety trials as affected by variable type, modelling strategy and sampling location17
VA-TIRFM-based SM kymograph analysis for dwell time and colocalization of plasma membrane protein in plant cells16
Targeted gene deletion with SpCas9 and multiple guide RNAs in Arabidopsis thaliana: four are better than two16
Roughness measurement of leaf surface based on shape from focus15
A device for the controlled cooling and freezing of excised plant specimens during magnetic resonance imaging15
Metric learning for image-based flower cultivars identification15
Improved estimation of aboveground biomass of regional coniferous forests integrating UAV-LiDAR strip data, Sentinel-1 and Sentinel-2 imageries15
A deep learning approach to track Arabidopsis seedlings’ circumnutation from time-lapse videos15
TreeJ: an ImageJ plugin for interactive cell lineage reconstruction from static images15
Simultaneous determination of protoporphyrin IX and magnesium protoporphyrin IX in Arabidopsis thaliana and Camellia sinensis using UPLC-MS/MS15
A robust and efficient automatic method to segment maize FASGA stained stem cross section images to accurately quantify histological profile15
Mix-and-match: an improved, fast and accessible protocol for hypocotyl micrografting of Arabidopsis seedlings with systemic ACC responses as a case study15
Non-destructive real-time monitoring of underground root development with distributed fiber optic sensing15
Full-length transcriptome sequences by a combination of sequencing platforms applied to isoflavonoid and triterpenoid saponin biosynthesis of Astragalus mongholicus Bunge15
Efficient transformation of the isolated microspores of Chinese cabbage (Brassica rapa L. ssp. pekinensis) by particle bombardment15
One pattern analysis (OPA) for the quantitative determination of protein interactions in plant cells15
Micro computed tomography analysis of barley during the first 24 hours of germination15
A model for genuineness detection in genetically and phenotypically similar maize variety seeds based on hyperspectral imaging and machine learning14
Fast estimation of plant growth dynamics using deep neural networks14
Segmentation and counting of wheat spike grains based on deep learning and textural feature14
Early detection of verticillium wilt in eggplant leaves by fusing five image channels: a deep learning approach14
Efficient isolation and purification of tissue-specific protoplasts from tea plants (Camellia sinensis (L.) O. Kuntze)14
A cassava common mosaic virus vector for virus-induced gene silencing in cassava14
An efficient grafting method for phytoplasma transmission in Catharanthus roseus14
The semi-automated development of plant cell wall finite element models14
Profiling of 1-aminocyclopropane-1-carboxylic acid and selected phytohormones in Arabidopsis using liquid chromatography-tandem mass spectrometry13
Optimizing speed breeding and seed/pod chip based genotyping techniques in pigeonpea: A way forward for high throughput line development13
Soybean cyst nematode detection and management: a review13
Highly efficient Agrobacterium rhizogenes-mediated hairy root transformation for gene functional and gene editing analysis in soybean13
Phenotyping seedlings for selection of root system architecture in alfalfa (Medicago sativa L.)13
Semi-supervised few-shot learning approach for plant diseases recognition13
A method to experimentally clamp leaf water content to defined values to assess its effects on apoplastic pH13
Determining water status of walnut orchards using the crop water stress index and canopy temperature measurements13
Improving the prediction performance of leaf water content by coupling multi-source data with machine learning in rice (Oryza sativa L.)13
Complementarity of Raman and Infrared spectroscopy for rapid characterization of fucoidan extracts13
Qualitative and quantitative evaluation of thylakoid complexes separated by Blue Native PAGE13
Identifying and mapping individual medicinal plant Lamiophlomis rotata at high elevations by using unmanned aerial vehicles and deep learning13
Effects of cushion box and closed let-down ladder usage on damage to corn during handling: physiological deterioration12
Orthogonal control of gene expression in plants using synthetic promoters and CRISPR-based transcription factors12
A streamlined guide RNA screening system for genome editing in Sorghum bicolor12
Applying quantitative spatial phenotypes analysis to the investigation of peltate glandular trichomes development pattern in Perilla frutescens12
Optimized synthesis of layered double hydroxide lactate nanosheets and their biological effects on Arabidopsis seedlings12
Optimization of ribosome profiling in plants including structural analysis of rRNA fragments12
The genetic puzzle of multicopy genes: challenges and troubleshooting12
Leaf area index estimation model for UAV image hyperspectral data based on wavelength variable selection and machine learning methods12
Rice bacterial blight resistant cultivar selection based on visible/near-infrared spectrum and deep learning11
An exploration of the influence of ZnO NPs treatment on germination of radish seeds under salt stress based on the YOLOv8-R lightweight model11
Accelerating leaf area measurement using a volumetric approach11
A novel in-situ-process technique constructs whole circular cpDNA library11
A cell P system with membrane division and dissolution rules for soybean leaf disease recognition11
A simple and efficient method to quantify the cell parameters of the seed coat, embryo and silique wall in rapeseed11
Mechanism of self-recovery of hydrophobicity after surface damage of lotus leaf11
Identifying rice field weeds from unmanned aerial vehicle remote sensing imagery using deep learning11
Correction: A deep learning model for predicting risks of crop pests and diseases from sequential environmental data11
Clarification of the infection pattern of Xanthomonas citri subsp. citri on citrus fruit by artificial inoculation11
Correction: Rapid and cost-effective molecular karyotyping in wheat, barley, and their crossprogeny by chromosome-specific multiplex PCR11
Gray mold and anthracnose disease detection on strawberry leaves using hyperspectral imaging11
Semantic segmentation of plant roots from RGB (mini-) rhizotron images—generalisation potential and false positives of established methods and advanced deep-learning models11
Quantitative MRI imaging of parenchyma and venation networks in Brassica napus leaves: effects of development and dehydration11
Correction: Soybean seed pest damage detection method based on spatial frequency domain imaging combined with RL-SVM11
Field cabbage detection and positioning system based on improved YOLOv8n11
Chemoinformatics-driven classification of Angiosperms using sulfur-containing compounds and machine learning algorithm10
A new biotechnology for in-planta gene editing and its application in promoting flavonoid biosynthesis in bamboo leaves10
Wheat leaf area index prediction using data fusion based on high-resolution unmanned aerial vehicle imagery10
Revolutionizing automated pear picking using Mamba architecture10
A toolkit to rapidly modify root systems through single plant selection10
PhenoTrack3D: an automatic high-throughput phenotyping pipeline to track maize organs over time10
A pin-based probe for electronic moisture meters to determine moisture content in a single wheat kernel10
Establishment of an efficient cotton root protoplast isolation protocol suitable for single-cell RNA sequencing and transient gene expression analysis10
Local mapping of root orientation traits by X-ray micro-CT and 3d image analysis: A study case on carrot seedlings grown in simulated vs real weightlessness10
UAV rice panicle blast detection based on enhanced feature representation and optimized attention mechanism10
UAS-based MT-YOLO model for detecting missed tassels in hybrid maize detasseling10
Computational fluid dynamics simulation experimental verification and analysis of droplets deposition behaviour on vibrating pear leaves10
Rapid in-solution preparation of somatic and meiotic plant cell nuclei for high-quality 3D immunoFISH and immunoFISH-GISH10
Advances in micropropagation, somatic embryogenesis, somatic hybridizations, genetic transformation and cryopreservation for Passiflora improvement10
3D-CNN detection of systemic symptoms induced by different Potexvirus infections in four Nicotiana benthamiana genotypes using leaf hyperspectral imaging10
A rapid and efficient in vivo inoculation method for introducing tree stem canker pathogens onto leaves: suitable for large-scale assessment of resistance in poplar breeding progeny10
Early real-time detection algorithm of tomato diseases and pests in the natural environment10
Optimization of the selection of suitable harvesting periods for medicinal plants: taking Dendrobium officinale as an example10
Segmentation and characterization of macerated fibers and vessels using deep learning9
Standardization of electrolyte leakage data and a novel liquid nitrogen control improve measurements of cold hardiness in woody tissue9
RiboPlotR: a visualization tool for periodic Ribo-seq reads9
A fast method to evaluate in a combinatorial manner the synergistic effect of different biostimulants for promoting growth or tolerance against abiotic stress9
Hyperspectral imaging for pest symptom detection in bell pepper9
Maize-IAS: a maize image analysis software using deep learning for high-throughput plant phenotyping9
Cryopreservation of Abies alba × A. numidica and Pinus nigra embryogenic tissues by stepwise dehydration method9
A procedure for Dex-induced gene transactivation in Arabidopsis ovules9
An improved bacterial mRNA enrichment strategy in dual RNA sequencing to unveil the dynamics of plant-bacterial interactions9
A highly efficient regeneration, genetic transformation system and induction of targeted mutations using CRISPR/Cas9 in Lycium ruthenicum9
An open-source controller to build a dynamic light intensity setup9
Innovation of strip fertilization planting for rice straw crushing with back-throwing and interrow-laying9
Texture feature extraction from microscope images enables a robust estimation of ER body phenotype in Arabidopsis8
Automated image registration of RGB, hyperspectral and chlorophyll fluorescence imaging data8
Two Arabidopsis promoters drive seed-coat specific gene expression in pennycress and camelina8
Four-dimensional measurement of root system development using time-series three-dimensional volumetric data analysis by backward prediction8
Combination of long-term 13CO2 labeling and isotopolog profiling allows turnover analysis of photosynthetic pigments in Arabidopsis leaves8
Atmospheric correction of vegetation reflectance with simulation-trained deep learning for ground-based hyperspectral remote sensing8
Novel in silico screening system for plant defense activators using deep learning-based prediction of reactive oxygen species accumulation8
Cucumber mosaic virus-induced gene and microRNA silencing in water dropwort (Oenanthe javanica (Blume) DC)8
A global non-invasive methodology for the phenotyping of potato under water deficit conditions using imaging, physiological and molecular tools8
X-ray driven peanut trait estimation: computer vision aided agri-system transformation8
ShinyCore: An R/Shiny program for establishing core collection based on single nucleotide polymorphism data8
GRABSEEDS: extraction of plant organ traits through image analysis8
Evaporative flux method of leaf hydraulic conductance estimation: sources of uncertainty and reporting format recommendation8
Non-wounding contact-based Inoculation of fruits with fungal pathogens in postharvest8
RhizoMAP: a comprehensive, nondestructive, and sensitive platform for metabolic imaging of the rhizosphere8
Crossing multiple life stages: fine-grained classification of agricultural pests8
High-throughput estimation of allele frequencies using combined pooled-population sequencing and haplotype-based data processing7
Meta-analysis of the role of zinc in coordinating absorption of mineral elements in wheat seedlings7
New improvements in grapevine genome editing: high efficiency biallelic homozygous knock-out from regenerated plantlets by using an optimized zCas9i7
DNA-free high-quality RNA extraction from 39 difficult-to-extract plant species (representing seasonal tissues and tissue types) of 32 families, and its validation for downstream molecular application7
‘Single-Seed-SpeedBulks:’ a protocol that combines ‘speed breeding’ with a cost-efficient modified single-seed descent method for rapid-generation-advancement in oat (Avena sativa L.)7
Yield estimation of high-density cotton fields using low-altitude UAV imaging and deep learning7
Characterization of leaf surface phenotypes based on light interaction7
A technical guide to TRITEX, a computational pipeline for chromosome-scale sequence assembly of plant genomes7
Whole mount multiplexed visualization of DNA, mRNA, and protein in plant-parasitic nematodes7
Rapid non-destructive method to phenotype stomatal traits7
Cucumber pathogenic spores’ detection using the GCS-YOLOv8 network with microscopic images in natural scenes7
Low-cost and automated phenotyping system “Phenomenon” for multi-sensor in situ monitoring in plant in vitro culture7
A comprehensive tool in recycling plant-waste of Gossypium barbadense L agricultural and industrial waste extracts containing gossypin and gossypol: hepatoprotective, anti-inflammatory and antioxidant7
Exploring genetic variability under extended photoperiod in lentil (Lens Culinaris Medik): vegetative and phenological differentiation according to genetic material’s origins7
MGIDI: a powerful tool to analyze plant multivariate data7
Deep learning for detecting herbicide weed control spectrum in turfgrass7
Development of a highly efficient virus-free regeneration system of Salvia miltiorrhiza from Sichuan using apical meristem as explants7
Synchrotron tomography applications in agriculture and food sciences research: a review7
Targeted mRNA demethylation in Arabidopsis using plant m6A editor7
Advancing virus-induced gene silencing in sunflower: key factors of VIGS spreading and a novel simple protocol7
Improved wood species identification based on multi-view imagery of the three anatomical planes7
A rapid assay for assessing bacterial effects on Arabidopsis thermotolerance7
Image analysis and polyphenol profiling unveil red-flesh apple phenotype complexity7
A novel approach integrating multispectral imaging and machine learning to identify seed maturity and vigor in smooth bromegrass7
Feature enhancement guided network for yield estimation of high-density jujube7
Dual discriminator GAN-based synthetic crop disease image generation for precise crop disease identification7
Computational fluid dynamics simulation analysis of the effect of curved rice leaves on the deposition behaviour of droplets7
Untapped potential of non-conventional rubus species: bioactivity, nutrition, and livelihood opportunities7
Establishment of Agrobacterium-mediated genetic transformation and application of CRISPR/Cas9 genome-editing system to Brassica rapa var. rapa6
Developing non-invasive 3D quantificational imaging for intelligent coconut analysis system with X-ray6
Establishment of an NPK nutrient monitor system in yield-graded cotton petioles under drip irrigation6
Non-destructive wood identification using X-ray µCT scanning: which resolution do we need?6
Assembly and operation of an imaging system for long-term monitoring of bioluminescent and fluorescent reporters in plants6
Earbox, an open tool for high-throughput measurement of the spatial organization of maize ears and inference of novel traits6
Overexpressing Arabidopsis thaliana ACBP6 in transgenic rapid-cycling Brassica napus confers cold tolerance6
The rapid-tome, a 3D-printed microtome, and an updated hand-sectioning method for high-quality plant sectioning6
ScAnalyzer: an image processing tool to monitor plant disease symptoms and pathogen spread in Arabidopsis thaliana leaves6
Robust CRW crops leaf disease detection and classification in agriculture using hybrid deep learning models6
Optimization of preparation and transformation of protoplasts from Populus simonii × P. nigra leaves and subcellular localization of the major latex protein 328 (MLP328)6
PlantNh-Kcr: a deep learning model for predicting non-histone crotonylation sites in plants6
Optimization of a rapid, sensitive, and high throughput molecular sensor to measure canola protoplast respiratory metabolism as a means of screening nanomaterial cytotoxicity6
Development of an infiltration-based RNA preservation method for cryogen-free storage of leaves for gene expression analyses in field-grown plants6
Soybean seed pest damage detection method based on spatial frequency domain imaging combined with RL-SVM6
Inter-row navigation line detection for cotton with broken rows6
WeCoNET: a host–pathogen interactome database for deciphering crucial molecular networks of wheat-common bunt cross-talk mechanisms6
Quality assurance of hyperspectral imaging systems for neural network supported plant phenotyping6
Seed protein biotyping in Amaranthus species: a tool for rapid identification of weedy amaranths of concern6
Improving multi-scale detection layers in the deep learning network for wheat spike detection based on interpretive analysis6
Enabling high-throughput quantitative wood anatomy through a dedicated pipeline6
LysipheN: a gravimetric IoT device for near real-time high-frequency crop phenotyping: a case study on common beans6
Comparing DNA isolation methods for forest trees: quality, plastic footprint, and time-efficiency6
Revisiting superiority and stability metrics of cultivar performances using genomic data: derivations of new estimators6
Fast-track transformation and genome editing in Brachypodium distachyon6
A high-throughput phenotyping method for sugarcane rind penetrometer resistance and breaking force characterization by near-infrared spectroscopy6
Root-TRAPR: a modular plant growth device to visualize root development and monitor growth parameters, as applied to an elicitor response of Cannabis sativa6
Variation in forest root image annotation by experts, novices, and AI6
Quantification of the fungal pathogen Didymella segeticola in Camellia sinensis using a DNA-based qRT-PCR assay6
High-resolution spectral information enables phenotyping of leaf epicuticular wax in wheat6
Development and application of a virus-induced gene silencing protocol for the study of gene function in narrow-leafed lupin5
Optimizing the photon ratio of red, green, and blue LEDs for lettuce seedlings: a mixture design approach5
Mapping intron retention events contributing to complex traits using splice quantitative trait locus5
An efficient CRISPR-Cas9 enrichment sequencing strategy for characterizing complex and highly duplicated genomic regions. A case study in the Prunus salicina LG3-MYB10 genes cluster5
Analysis of canopy phenology in man-made forests using near-earth remote sensing5
Automatic 3D cell segmentation of fruit parenchyma tissue from X-ray micro CT images using deep learning5
Non-destructive pre-symptomatic detection of gray mold infection in kiwifruit using hyperspectral data and chemometrics5
Development of a semi-open chamber system for the gas exchange measurement of whole-canopy under steady and unsteady states in cucumber seedlings5
A comparative study on point cloud down-sampling strategies for deep learning-based crop organ segmentation5
Quantification of spatial metal accumulation patterns in Noccaea caerulescens by X-ray fluorescence image processing for genetic studies5
Automated quantification of fluorescence and morphological changes in pretreated wood cells by fluorescence macroscopy5
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