Journal of Biological Engineering

Article	Citations
Nano-size tantalum particles suppress the osteogenic activity of osteoblasts and stimulate osteoclasts	338
Cerium-doped calcium carbonate microparticles combined with low-intensity ultrasound for efficient sonodynamic therapy in body sculpting	110
A detoxified exosome–ECM hydrogel depot for localized and sustained chemotherapy in prostate cancer	104
Gene syntaxes modulate gene expression and circuit behavior on plasmids	95
Self-healing PVA/PVP micelle–hydrogel composite system for potential phenytoin repositioning in wound healing	86
Droplet microfluidic screening to engineer angiotensin-converting enzyme 2 (ACE2) catalytic activity	73
Advanced metabolic Engineering strategies for the sustainable production of free fatty acids and their derivatives using yeast	65
Promoting osteogenesis and bone regeneration employing icariin-loaded nanoplatforms	62
Verification and application of an automated real-time antimicrobial susceptibility testing system: for accelerated antibiotic susceptibility testing and high-throughput screening of antibiotic synerg	56
Combinatorial engineering pinpoints shikimate pathway bottlenecks in para-aminobenzoic acid production in Pseudomonas putida	55
3D-printed PCL@BG scaffold integrated with SDF-1α-loaded hydrogel for enhancing local treatment of bone defects	51
Osteogenic differentiation of adipose-derived stem cells on dihydroartemisinin electrospun nanofibers	50
New approach methodologies for in vitro toxicity screening of nanomaterial using a pulmonary three-dimensional floating extracellular matrix model	46
3D-printed nanohydroxyapatite/methylacrylylated silk fibroin scaffold for repairing rat skull defects	44
Core-shell nanofiber dressings with zinc oxide nanoparticles and cell-free fat extract: boosting fibroblast activity and antibacterial efficacy	42
Cell-cultivated aquatic food products: emerging production systems for seafood	40
Waste-to-sensor upcycling of polyethylene terephthalate over Ag/Zr-MOF photocatalyst for microplastic degradation and AI-assisted heavy metal detection	40
Role of mesenchymal stem cell-derived exosomes in the regeneration of different tissues	38
Integrated genomic and transcriptomic Insights into methanol tolerance mechanisms in Methylobacterium extorquens AM1, identifying key targets for strain engineering	38
Comparison of different decellularization protocols for porcine centrum tendineum diaphragmatis and diaphragmatic muscle – a base for effective recellularization	36
A new approach to off-gas analysis for shaken bioreactors showing high CTR and RQ accuracy	35
Research progress of biomimetic materials in oral medicine	33
A CRISPR interference strategy for gene expression silencing in multiple myeloma cell lines	28
RN-Autoencoder: Reduced Noise Autoencoder for classifying imbalanced cancer genomic data	26
Integration of TLR7/8 agonists into lipid nanoparticles enhances antigen-specific immune responses to N1-methyl-Ψ-modified mRNA-LNP vaccines	25

Codeine 3-O-demethylase catalyzed biotransformation of morphinan alkaloids in Escherichia coli: site directed mutagenesis of terminal residues improves enzyme expression, stability and biotransformati	24
Quantitative imaging of magnetic nanoparticles in an unshielded environment using a large AC susceptibility array	24
Bio-engineered thermo-sensitive alginate/PNIA-g-CS co-polymeric injectable hydrogel laden with GDF-5 to stimulate nucleus pulposus for IVD regeneration	23
Tissue engineering in age-related macular degeneration: a mini-review	23
Enhancing chemical and physical stability of pharmaceuticals using freeze-thaw method: challenges and opportunities for process optimization through quality by design approach	22
Influence of shear force on ex vivo expansion of hematopoietic model cells in a stirred tank bioreactor	22
Automated classification of urine biomarkers to diagnose pancreatic cancer using 1-D convolutional neural networks	22
Harnessing plant chloroplasts for oral delivery of a multi-epitope HPV vaccine: toward cost-effective systemic and mucosal immunization	21
Efficient site-specific recombination by self-activating split-Dre recombinase in mammalian cells and E. coli	21
Fabricating a robust POSS-PCL nanofiber scaffold for nesting of mesenchymal stem cells: potential application in bone tissue regeneration	21
Chitosan modified with PAP as a promising delivery system for melatonin in the treatment of osteoporosis: targeting the divalent metal transporter 1	20
Exploring of therapeutic potential of indirubin-loaded nanofibrous scaffolds for localized melanoma treatment	20
DC electrical stimulation enhances proliferation and differentiation on N2a and MC3T3 cell lines	19
Engineered Metal-Organic Frameworks (MOFs)-electrospun nanofibers for wound healing: a review	19
Microfluidics-based cell recognition through optimizing suspended cell staining techniques and artificial intelligence	19
Thiol release improvement in Saccharomyces cerevisiae oenological strains by CRISPR/Cas9-based IRC7 molecular cisgenesis	19
The lyophilized chloroplasts store synthetic DARPin G3 as bioactive encapsulated organelles	19
Nonthermal biocompatible plasma in stimulating osteogenic differentiation by targeting p38/ FOXO1 and PI3K/AKT pathways in hBMSCs	19
Rapid osteoinduction of human adipose-derived stem cells grown on bioactive surfaces and stimulated by chemically modified media flow	19
Biology-inspired engineering for circular bioeconomy systems	18
High-throughput proliferation and activation of NK-92MI cell spheroids via a homemade one-step closed bioreactor in pseudostatic cultures for immunocellular therapy	18
An in vitro culture platform for studying the effect of collective cell migration on spatial self-organization within induced pluripotent stem cell colonies	18
Smartphone-based multispectral autofluorescence analysis of bacteria mixtures of staphylococci using convolutional neural network	18
Identification and verification of diagnostic biomarkers for deep infiltrating endometriosis based on machine learning algorithms	18
Rapid remodeling observed at mid-term in-vivo study of a smart reinforced acellular vascular graft implanted on a rat model	17
Design and performance analysis of bionic trabecular bone scaffold structure based on Voronoi	17
Role of viscoelasticity in the appearance of low-Reynolds turbulence: considerations for modelling	17
Engineered chitosan/HP-β-CD hydrogel for targeted co-delivery of curcumin and berberine for the treatment of ulcerative colitis	17
Unraveling pathogenesis, biomarkers and potential therapeutic agents for endometriosis associated with disulfidptosis based on bioinformatics analysis, machine learning and experiment validation	16
Microfluidic chip and isothermal amplification technologies for the detection of pathogenic nucleic acid	16
Tissue engineering RPE sheet derived from hiPSC-RPE cell spheroids supplemented with Y-27632 and RepSox	15
Biomaterial-based chitosan nanohydrogel films: combination of Bistorta officinalis and Ca-doped carbon dots for improved blood clotting	15
Optimization of a recombinant BlaR-CTD protein formulation using the response surface methodology	15
Biomaterials for fetal membrane repair in preterm premature rupture of membranes: advances in tissue engineering strategies	15
CRISPRi-assisted metabolic engineering of cyanobacteria for photosynthetic hyaluronic acid from CO2	15
Effect of recombinant protein production and release on microalgal fitness and the impact of environmental conditions for localized therapeutic delivery	15
Beyond linearity: reimagining AI as a participant in circular bioeconomies	14
Quantitative impedance-based characterization of breast cancer cell migration and metastatic potential	14
Genetically engineered secretory horseradish peroxidase is a sensitive, stable, and affordable non-lytic reporter gene system for real-time promoter activity management	14
Eggshell waste bioprocessing for sustainable acid phosphatase production and minimizing environmental hazards	14
Clinical utility of mesenchymal stem/stromal cells in regenerative medicine and cellular therapy	14
Microfluidic device for enhancement and analysis of osteoblast differentiation in three-dimensional cell cultures	13
Efflux pump knockout minimizes crosstalk and boosts response in transcription factor-based biosensors	13
Early introduction of 3D modeling modules promotes the development of simulation skills in downstream biomedical engineering curricula	13
Dual-responsive synthetic gene circuit for dynamic biologic drug delivery via inflammatory and circadian signaling pathways	12
Enhanced methanol tolerance and utilization in Pichia pastoris revealed by adaptive laboratory evolution	12
Cloning, expression, and one-step purification/immobilization of two carbohydrate-binding module-tagged alcohol dehydrogenases	12
Therapeutic efficacy of mesenchymal stem cell-derived extracellular vesicles combined with injectable hydrogels in bone defect repair: a systematic review and meta-analysis of preclinical studies	12
Two-color spheroid model for determining the O2-induced radiosensitivity of HNSCC	12
Engineering probiotic Escherichia coli for inflammation-responsive indoleacetic acid production using RiboJ-enhanced genetic circuits	12

Codon usage modulates the relationship between the burden and yield of protein overexpression	12
Applications of carbon dots and its modified carbon dots in bone defect repair	12
Correction: Engineered hypoxia-responding Escherichia coli carrying cardiac peptide genes, suppresses tumor growth, angiogenesis and metastasis in vivo	12
High-titer 2-phenylethanol production in Escherichia coli via decarboxylation-step optimization and a vegetable-oil overlay for biocompatible in situ recovery	12
Customized biofilm device for antibiofilm and antibacterial screening of newly developed nanostructured silver and zinc coatings	12
Correction to: Challenges and progress of neurodrug: bioactivities, production and delivery strategies of nerve growth factor protein	11
Exosome-functionalized collagen-coated 3D-printed PCL scaffold for enhanced osteogenic differentiation and bone regeneration: an in vitro and in vivo study	11
Enhancing osteogenic differentiation of dental pulp stem cells through rosuvastatin loaded niosomes optimized by Box-Behnken design and modified by hyaluronan: a novel strategy for improved efficiency	11
Enhancing bioprocessing of red pigment from immobilized culture of gamma rays mutant of the endophytic fungus Monascus ruber SRZ112	11
A novel inductively coupled capacitor wireless sensor system for rapid antibiotic susceptibility testing	11
NIR-activated catechol-functionalized nanodiamond nanofibers for accelerating on-demand MRSA and E. coli biofilm eradication	11
Bakuchiol-loaded amphiphilic polymeric nanoparticles: enhancing stability and multifunctionality through encapsulation	11
Combined drugs-loaded nano-lipodisk carrier for pH-responsive delivery for improved therapeutic potential on lung cancer model	11
Phytoconstituents of Withania somnifera (L.) Dunal (Ashwagandha) unveiled potential cerebroside sulfotransferase inhibitors: insight through virtual screening, molecular dynamics, toxicity, and revers	11
Fluorescent tools for the standardized work in Gram-negative bacteria	11
Chitin nanowhiskers/octacalcium phosphate synergistically reinforced chitosan hydrogel for enhanced bone regeneration	10
Bilayer osteochondral graft in rabbit xenogeneic transplantation model comprising sintered 3D-printed bioceramic and human adipose-derived stem cells laden biohydrogel	10
Application of extracellular vesicles in the CRISPR-based diagnosis and treatment: possibilities and challenges	10
Seaweed-based alginate/hydroxyapatite composite for the effective removal of bacteria, cyanobacteria, algae, and crystal violet from water	10
3D printed zirconia used as dental materials: a critical review	10
Residual-hybrid dynamic response-surface/machine-learning framework for predicting malachite green removal and biodegradation by a bacterial consortium	10
A dual-inducible control system for multistep biosynthetic pathways	10
Anti-inflammatory activity of magnetic fields emitted by graphene devices on cultured human cells	10
Enhancing in vitro osteogenic differentiation of mesenchymal stem cells via sustained dexamethasone delivery in 3D-Printed hybrid scaffolds based on polycaprolactone-nanohydroxyapatite/alginate-gelati	9
Engineering the T6SS of Pseudomonas for targeted delivery of antibacterial and antifungal effectors	9
Insect cell expression system: advances in applications, engineering strategies, and bioprocess development	9
Correction: Follicular reconstruction and neo-oogenesis in xenotransplantation of human ovarian isolated cells derived from chemotherapy-induced POF patients	9
Chitosan as a promising materials for the construction of nanocarriers for diabetic retinopathy: an updated review	9
Real time monitoring of hydrogenotrophic methanogenesis under deep saline aquifers conditions	9
Reprogramme the E. coli metabolism by engineering a functional carbon-fixation pathway	9
Soil microbiome characterization and its future directions with biosensing	9
Optimizing the fabrication of a 3D high-resolution implant for neural stimulation	9
Light scattering-based screening method for rapid evaluating antibiotic effects on bacteria using laser speckle imaging	9
Exosome-loaded hydrogels for bone regeneration: a cell-free therapeutic strategy	9
Lactic acid bacteria as microbial cell factories for the in vivo delivery of therapeutic proteins as secretable TAT fusion products	9
Genetically encoded biosensor enabled mining, characterisation and engineering of aromatic acid MFS transporters	9
Construction of bispecific antibodies by specific pairing between the heavy chain and the light chain using removable SpyCatcher/SnoopCatcher units	9
Elucidating the metabolic landscape of Bacillus velezensis WY-65 in basic and cocktail nutrient media: a biocontrol approach against peanut web blotch	9
Thrombomodulin is essential for recombinant Fc-fusion protein production in Chinese hamster ovary cells via multiple signaling pathways	8
An engineered ligand-responsive Csy4 endoribonuclease controls transgene expression from Sendai virus vectors	8
Challenges of aortic valve tissue culture – maintenance of viability and extracellular matrix in the pulsatile dynamic microphysiological system	8
Unravelling the mechanotransduction pathways in Alzheimer’s disease	8
Pathway reconstruction of Corynebacterium glutamicum for C30 carotenoid 4,4′-diaponeurosporene production	8
Non-spherical gold nanoparticles enhanced fluorescence of carbon dots for norovirus-like particles detection	8
Light regulation of secondary metabolism in fungi	8
Application of microneedle patches in the alleviation of cardiac infarction in animal models: a systematic review and meta-analysis	8
Gentamicin-loaded chitosan/folic acid-based carbon quantum dots nanocomposite hydrogel films as potential antimicrobial wound dressing	7
A novel computationally engineered collagenase reduces the force required for tooth extraction in an ex-situ porcine jaw model	7
High throughput mutagenesis and screening for yeast engineering	7
A continuous viral vaccine biomanufacturing platform utilizing multiple bioreactor configurations	7
Breast cancer brain metastasis: from etiology to state-of-the-art modeling	7
Extracellular vesicles for macrophage reprogramming: an emerging paradigm in immunomodulatory therapeutics	7
Multilayered blow-spun vascular prostheses with luminal surfaces in Nano/Micro range: the influence on endothelial cell and platelet adhesion	7
Robocast Zn- and Co-doped bioactive glass/tricalcium phosphate scaffolds for bone regeneration	7
Human preclinical multiple myeloma in vitro models for disease modeling and therapy screening	7
The progressive trend of modeling and drug screening systems of breast cancer bone metastasis	7
Are we there yet? The road to faster and more efficient CAR T cell manufacturing	7
Hypoxia and re-oxygenation effects on human cardiomyocytes cultured on polycaprolactone and polyurethane nanofibrous mats	6
High-throughput strategies for monoclonal antibody screening: advances and challenges	6
Simultaneous multigene integration in Aspergillus fumigatus using CRISPR/Cas9 and endogenous counter-selectable markers	6
Exosomes derived from heat shock preconditioned bone marrow mesenchymal stem cells alleviate cisplatin-induced ototoxicity in mice	6
Label-free multimodal electro-thermo-mechanical (ETM) phenotyping as a novel biomarker to differentiate between normal, benign, and cancerous breast biopsy tissues	6
Epithelial cell-cell interactions in an overcrowded environment: jamming or live cell extrusion	6
Serum-free endothelial cell culture medium for vascular smooth muscle cells sheet formation	6
Engineering NKG2D ligand affinity transforms EGFR-targeted NK cell engagers into high-potency effectors against pancreatic cancer	6
Mixed culture biocatalytic production of the high-value biochemical 7-methylxanthine	6
Advances in the application of gas vesicles in medical imaging and disease treatment	6
An electrochemical biosensor for the detection of microRNA-31 as a potential oral cancer biomarker	6
Development of a novel defined minimal medium for Gluconobacter oxydans 621H by systematic investigation of metabolic demands	6
Streamlined purification of ADDomer nanoparticles for scalable biomanufacturing	6
Rapid, simple, and effective strategy to produce monoclonal antibodies targeting protein structures using hybridoma technology	6
Metabolic engineering of Escherichia coli BW25113 for the production of 5-Aminolevulinic Acid based on CRISPR/Cas9 mediated gene knockout and metabolic pathway modification	6
Lactate-related biomarkers in severe acute pancreatitis: insights from machine learning, molecular dynamics, and experimental validation	6
Exosome-mediated crosstalk between epithelial cells amplifies the cell injury cascade in CaOx stone formation	5
A novel chassis for high-quality recombinant protein expression: apoptosis pathway engineering via multi-gene knockout and efficacy improvement of CHO cells	5
Exploring the impact of taurine on the biochemical properties of urate oxidase: response surface methodology and molecular dynamics simulation	5
Hyperactivation of crosslinked lipases in elastic hydroxyapatite microgel and their properties	5
Effect of HIF-1α inhibitor LW6 on chondrogenic properties of mesenchymal stromal cells and chondrocytes in cell sheets under physioxia	5
The construction of the gelatin methacrylate microneedle -encapsulated newcastle disease virus and its antitumor effect in hepatocellular carcinoma	5
Universal and quantitative detection of double-stranded RNAs as a signature of pan-virus infections using a luciferase-based biosensor	5

Hybrid metabolic engineering enables xylose-driven co-production of polyhydroxybutyrate and violacein in Escherichia coli	5
Medium dependent factors govern the functionality of engineered type III secretion systems	5
CIRCLE-F/V: a dual-mode CRISPR-Cas13a cascade biosensor for ultrasensitive and visual detection of low-abundance EGFR mutations	5
Targeting the PEDV 3CL protease for identification of small molecule inhibitors: an insight from virtual screening, ADMET prediction, molecular dynamics, free energy landscape, and binding energy calc	5
Simultaneous online monitoring of viscosity and oxygen transfer rate in shake flask cultures	5
Continuous secretory production in E. coli enables scalable, high-titer manufacturing of active recombinant endonucleases	5
Construction of Escherichia coli L-isoleucine cell factories based on propionate pathway	5
Scale-up of CHO cell cultures: from 96-well-microtiter plates to stirred tank reactors across three orders of magnitude	5
Biomolecular and cellular effects in skin wound healing: the association between ascorbic acid and hypoxia-induced factor	5
An innovative cholesteric liquid crystal biosensor enabling high-contrast colorimetric detection and haze-based quantitation	5
Proteomic analysis of decellularized mice liver and kidney extracellular matrices	4
Metabolic engineering of Komagataella phaffii for enhanced 3-hydroxypropionic acid (3-HP) production from methanol	4
Dual-drug (Curcumin/Ciprofloxacin) loading and release from chitosan-based hydrogels embedded with magnetic Montmorillonite/Hyaluronic acid for enhancing wound healing	4
Reversible fluorescent solid porous films for detection of zinc ions in biological media	4
Decellularized apple hypanthium as a plant-based biomaterial for cartilage regeneration in vitro: a comparative study of progenitor cell types and environmental conditions	4
Novel organoid construction strategy for non-involuting congenital hemangioma for drug validation	4
Synthesizing hybrid copper phosphate (Cu3(PO4)2) nanoflowers using Cu+2 and shed snakeskin: antioxidant, antibacterial, anticancer, guaiacol, anionic, and cationic dye degradation properties	4
From microbial proteins to cultivated meat for alternative meat-like products: a review on sustainable fermentation approaches	4
Metabolic engineering of Saccharomyces cerevisiae for the biosynthesis of a fungal pigment from the phytopathogenic fungus Cladosporium phlei	4
Nucleic acid direct delivery to fibroblasts: a review of nucleofection and applications	4
Investigating the impact of multinational collaborations on cultural understanding, health disparities, biomedical innovations, and professional development through project-based learning	4
Optimize the parameters for the synthesis by the ionic gelation technique, purification, and freeze-drying of chitosan-sodium tripolyphosphate nanoparticles for biomedical purposes	4
Systemic versus local delivery of mesenchymal stem cells to improve the early stages of fracture healing in a polytrauma model	4
Advanced biomanufacturing and evaluation of adeno-associated virus	4
Recent advances in nanomaterial-based biosensor for periodontitis detection	3
Engineered neutrophil engagers overcome IgA limitations and reprogram resting neutrophils for cancer immunotherapy	3
The infectivity of AAV9 is influenced by the specific location and extent of chemically modified capsid residues	3
A novel PNIPAM-Modified polyurethane/carboxymethyl cellulose photo-thermoresponsive hydrogel loaded with gemcitabine to suppress esophageal cancer cells via VEGF-mediated angiogenic pathway inhibition	3
Biosynthesis of ternary NiCoFe2O4 nanoflowers: investigating their 3D structure and potential use in gene delivery	3
Adaptive laboratory evolution with ethionine identifies novel genetic determinants for enhanced protein and methionine accumulation in Saccharomyces cerevisiae	3
Preparation of bilayer tissue-engineered polyurethane/poly-L-lactic acid nerve conduits and their in vitro characterization for use in peripheral nerve regeneration	3
Investigation of two metabolic engineering approaches for (R,R)-2,3-butanediol production from glycerol in Bacillus subtilis	3
A single-tube RAA-CRISPR-Cas12a diagnostic platform for rapid detection of Chlamydia trachomatis	3
Synthesis of metformin-derived fluorescent quantum dots: uptake, cytotoxicity, and inhibition in human breast cancer cells through autophagy pathway	3
LIFTOSCOPE: development of an automated AI-based module for time-effective and contactless analysis and isolation of cells in microtiter plates	3
Investigation of pH-dependent Paclitaxel delivery mechanism employing Chitosan-Eudragit bioresponsive nanocarriers: a molecular dynamics simulation	3
Nanozyme-based aptasensors for the detection of tumor biomarkers	3
PLGA/BGP/Nef porous composite restrains osteoclasts by inhibiting the NF-κB pathway, enhances IGF-1-mediated osteogenic differentiation and promotes bone regeneration	3
Mussel-inspired electroactive, antibacterial and antioxidative composite membranes with incorporation of gold nanoparticles and antibacterial peptides for enhancing skin wound healing	3
Treatment of effluents from Food Services Establishment (FSEs) by Physico-chemical processes: a case study for Trinidad & Tobago	3
A modular biodevice assembly tool kit for combinatorial screening and benchmarking multiple cell surface display platforms in Escherichia coli	3
Enhancing the therapeutic efficacy of gemcitabine in bladder cancer through TGF-β1 inhibition and pluronic F-127-based microsphere delivery	3
Termination sequence between an inducible promoter and ubiquitous chromatin opening element (UCOE) reduces gene expression leakage and silencing	3
Avoiding overflow metabolite formation in Komagataella phaffii fermentations to enhance recombinant protein production	3
Non-viral gene delivery to human mesenchymal stem cells: a practical guide towards cell engineering	3
The ELF3 transcription factor is associated with an epithelial phenotype and represses epithelial-mesenchymal transition	3
Evaluating ATP testing for distribution system monitoring: comparison to HPC, impact of chlorine quenching, and hold time dependency	3
Self-assembled PEGylated nanoparticles based on Elafibranor derivative 3d for the treatment of metabolic dysfunction-associated steatohepatitis	3
SsDiHal: discovery and engineering of a novel tryptophan dihalogenase	3
Co-expression of holin gene improves heterologous cellulase secretion and surface display by lactic acid bacteria Lactococcus cremoris	3
CRISPR/Cas12a-mediated gene silencing across diverse functional genes demonstrates single gene-specific spacer efficacy in Mycobacterium smegmatis	3