OOIR: Observatory of International Research

Papers

(The H4-Index of Nature Protocols is 65. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2022-08-01 to 2026-08-01.)

Article	Citations
Author Correction: cfSNV: a software tool for the sensitive detection of somatic mutations from cell-free DNA	1343
Open thoracic surgical implantation of cardiac pacemakers in rats	1124
ChromEMT: visualizing and reconstructing chromatin ultrastructure and 3D organization in situ	754
Fabrication of focused electron beam induced deposition tips for high-speed atomic force microscopy using benchtop scanning electron microscopy	571
Author Correction: Z-REX: shepherding reactive electrophiles to specific proteins expressed tissue specifically or ubiquitously, and recording the resultant functional electrophile-induced redox respo	513
Purification and functional characterization of novel human skeletal stem cell lineages	494
GPSeq maps the radial organization of eukaryotic genomes along the nuclear periphery–center axis	349
Automated cGMP optical labeling of FDA-approved antibodies for human use	324
Inducible CRISPR–Cas9 screening platform to interrogate non-proliferative cellular states	303
Patient-derived ependymoma and medulloblastoma tumoroids: generation, biobanking and drug screening	274
Isoform-specific RNA structure determination using Nano-DMS-MaP	270
Spontaneous and experimental models of lymph node metastasis	265
Expansion of human pluripotent stem cell–induced nephron progenitor cells (iNPCs) and the generation of nephron organoids from iNPCs	260
Visualization of metabolites and microbes at high spatial resolution using MALDI mass spectrometry imaging and in situ fluorescence labeling	231
Reproducible strategy for excisional skin-wound-healing studies in mice	225
Tutorial: biomembranes in hybrid living bioelectronics	221
Design and application of the transformer base editor in mammalian cells and mice	183
Generating human bone marrow organoids for disease modeling and drug discovery	162
GLORI for absolute quantification of transcriptome-wide m6A at single-base resolution	161
Open-vessel polymerization of N-carboxyanhydride (NCA) for polypeptide synthesis	156
Precise tracking of nanoparticles in plant roots	155
Photoimmunotechnology as a powerful biological tool for molecular-based elimination of target cells and microbes, including bacteria, fungi and viruses	150
Preparation of C4′-modified nucleoside analogs	138
Publisher Correction: In vivo pulse labeling of isochronic cohorts of cells in the central nervous system using FlashTag	135
Author Correction: Reliable detection of somatic mutations in solid tissues by laser-capture microdissection and low-input DNA sequencing	133

Author Correction: Chromatin integration labeling for mapping DNA-binding proteins and modifications with low input	130
Publisher Correction: Rapid reaction optimization by robust and economical quantitative benchtop 19F NMR spectroscopy	126
Metabolomics using anion-exchange chromatography mass spectrometry for the analysis of cells, tissues and biofluids	125
Quantitative molecular imaging using deep magnetic resonance fingerprinting	124
Photocatalytic low-temperature defluorination of PTFE	124
Simplified ischemia-free liver transplantation with continuous normothermic machine perfusion	122
Fabrication of thin-film electrodes and organic electrochemical transistors for neural implants	113
Fabrication of electron tunneling probes for measuring single-protein conductance	110
Sonicated inks and focused-ultrasound writing enable deep-penetration acoustic volumetric printing	105
Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds	101
Small RNA structural biochemistry in a post-sequencing era	100
Head-mounted central venous access during optical recordings and manipulations of neural activity in mice	98
Rapid reaction optimization by robust and economical quantitative benchtop 19F NMR spectroscopy	98
Spatially resolved epigenome sequencing via Tn5 transposition and deterministic DNA barcoding in tissue	97
Three-dimensional human neural culture on a chip recapitulating neuroinflammation and neurodegeneration	94
High-throughput fabrication of antimicrobial phage microgels and example applications in food decontamination	92
Preparation and biomedical applications of single-metal atom catalysts	90
Expansion and fluctuations-enhanced microscopy for nanoscale molecular profiling of cells and tissues	89
How to isolate channel-forming membrane proteins using the E. coli expression system	89
Collections from Nature Protocols	88
Large-scale growth of C. elegans and isolation of membrane protein complexes	88
Gene expression and chromatin state mapped in space	87
CODAvision: best practices and a user-friendly interface for rapid, customizable segmentation of medical images	87
Achieving transient and reversible optical transparency in live mice with tartrazine	85
Honey bee functional genomics using symbiont-mediated RNAi	85
A new era for coral functional genomics	78
A modular method for high-throughput measurement of ion channel currents in cardiac myocytes	77
mRNA lipid nanoparticle formulation, characterization and evaluation	77
A practical guide to identifying associations between tandem repeats and complex human traits using consensus genotypes from multiple tools	75
Expansion of human hepatocytes and their application in three-dimensional culture and genetic manipulation	74
Scalable growth of vertical graphene nanosheets by thermal chemical vapor deposition	73
Preparation and quantitative analysis of multicenter luminescence materials for sensing function	73
A versatile tissue-rolling technique for spatial-omics analyses of the entire murine gastrointestinal tract	73
Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility	73
Generation and long-term culture of human cerebellar organoids from pluripotent stem cells	73
Structural connectome construction using constrained spherical deconvolution in multi-shell diffusion-weighted magnetic resonance imaging	72
Sequential orthogonal assays for longitudinal and endpoint characterization of three-dimensional spheroids	71
Fibrous polyisocyanide hydrogels for 3D cell culture applications	68
A ‘print–pause–print’ protocol for 3D printing microfluidics using multimaterial stereolithography	67
Preparation of selective organ-targeting (SORT) lipid nanoparticles (LNPs) using multiple technical methods for tissue-specific mRNA delivery	66
Sonoafterglow nanoprobes for deep-tissue imaging of peroxynitrite	65