OOIR: Observatory of International Research

Papers

(The H4-Index of Nature Protocols is 68. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-04-01 to 2025-04-01.)

Article	Citations
An accessible workflow for high-sensitivity proteomics using parallel accumulation–serial fragmentation (PASEF)	993
A multimodal imaging pipeline to decipher cell-specific metabolic functions and tissue microenvironment dynamics	468
Circular Vectors as an efficient, fully synthetic, cell-free approach for preparing small circular DNA as a plasmid substitute for guide RNA expression in CRISPR–Cas9 genome editing	422
Seq-Scope: repurposing Illumina sequencing flow cells for high-resolution spatial transcriptomics	342
Multiplexed chromatin immunoprecipitation sequencing for quantitative study of histone modifications and chromatin factors	306
Induction of human trophoblast stem cells	296
Reproducible strategy for excisional skin-wound-healing studies in mice	259
Transcription factor chromatin profiling genome-wide using uliCUT&RUN in single cells and individual blastocysts	246
Stabilization and structure determination of integral membrane proteins by termini restraining	232
Snapshot photoacoustic topography through an ergodic relay of optical absorption in vivo	220
An LC-MS/MS assay and complementary web-based tool to quantify and predict compound accumulation in E. coli	218
Subretinal injection in mice to study retinal physiology and disease	190
Design and application of the transformer base editor in mammalian cells and mice	184
fMRI data acquisition and analysis for task-free, anesthetized rats	182
Scalable single-cell profiling of chromatin modifications with sciCUT&Tag	176
Modified Neuropixels probes for recording human neurophysiology in the operating room	171
Isocyanide-based multi-component reactions for carrier-free and carrier-bound covalent immobilization of enzymes	165
Improved sampling and DNA extraction procedures for microbiome analysis in food-processing environments	165
Purification and functional characterization of novel human skeletal stem cell lineages	161
In situ label-free X-ray imaging for visualizing the localization of nanomedicines and subcellular architecture in intact single cells	154
Quantification of SARS-CoV-2 neutralizing antibody by wild-type plaque reduction neutralization, microneutralization and pseudotyped virus neutralization assays	154
Rapid ex vivo molecular fingerprinting of biofluids using laser-assisted rapid evaporative ionization mass spectrometry	152
Bacterial genome engineering using CRISPR-associated transposases	150
CRISPR-Combo–mediated orthogonal genome editing and transcriptional activation for plant breeding	143
Genetic recording of in vivo cell proliferation by ProTracer	138

Author Correction: Z-REX: shepherding reactive electrophiles to specific proteins expressed tissue specifically or ubiquitously, and recording the resultant functional electrophile-induced redox respo	132
Advances in mass spectrometry to unravel the structure and function of protein condensates	131
Engineering megabase-sized genomic deletions with MACHETE (Molecular Alteration of Chromosomes with Engineered Tandem Elements)	128
Visualization of metabolites and microbes at high spatial resolution using MALDI mass spectrometry imaging and in situ fluorescence labeling	125
QurvE: user-friendly software for the analysis of biological growth and fluorescence data	124
Generation of proximal tubule-enhanced kidney organoids from human pluripotent stem cells	123
Author Correction: PepSeq: a fully in vitro platform for highly multiplexed serology using customizable DNA-barcoded peptide libraries	122
Design and construction of a microfluidics workstation for high-throughput multi-wavelength fluorescence and transmittance activated droplet analysis and sorting	121
BridGE: a pathway-based analysis tool for detecting genetic interactions from GWAS	114
Measuring carbohydrate recognition profile of lectins on live cells using liquid glycan array (LiGA)	112
OBSERVE: guidelines for the refinement of rodent cancer models	112
Assembly of a stem cell-derived human postimplantation embryo model	109
ChromEMT: visualizing and reconstructing chromatin ultrastructure and 3D organization in situ	106
Detect-seq, a chemical labeling and biotin pull-down approach for the unbiased and genome-wide off-target evaluation of programmable cytosine base editors	106
Author Correction: cfSNV: a software tool for the sensitive detection of somatic mutations from cell-free DNA	105
Generating human bone marrow organoids for disease modeling and drug discovery	104
Optimized whole-genome sequencing workflow for tumor diagnostics in routine pathology practice	104
Anaerobic cryoEM protocols for air-sensitive nitrogenase proteins	103
Publisher Correction: Generation of ‘semi-guided’ cortical organoids with complex neural oscillations	97
Generation of mouse–human chimeric embryos	95
RNA sample optimization for cryo-EM analysis	95
Capture of the newly transcribed RNA interactome using click chemistry	93
dCas9-VPR-mediated transcriptional activation of functionally equivalent genes for gene therapy	92
Multispectral confocal 3D imaging of intact healthy and tumor tissue using mLSR-3D	89
Peat-based gnotobiotic plant growth systems for Arabidopsis microbiome research	85
Open thoracic surgical implantation of cardiac pacemakers in rats	83
Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells	83
Applications of and considerations for using CRISPR–Cas9-mediated gene conversion systems in rodents	83
Rapid in situ identification of biological specimens via DNA amplicon sequencing using miniaturized laboratory equipment	82
Thrombin generation test based on a 96-channel pipettor for evaluation of FXIa procoagulant activity in pharmaceuticals	82
Cytosine and adenosine base editing in human pluripotent stem cells using transient reporters for editing enrichment	82
GLORI for absolute quantification of transcriptome-wide m6A at single-base resolution	80
Publisher Correction: A practical guide to large-scale docking	80
Comprehensive multiscale analysis of lactate metabolic dynamics in vitro and in vivo using highly responsive biosensors	78
Generation of heart-forming organoids from human pluripotent stem cells	78
Precise motor mapping with transcranial magnetic stimulation	74
Analysis of combinatorial CRISPR screens with the Orthrus scoring pipeline	73
Microtiter plate assays to assess antibiofilm activity against bacteria	73
Full-length circular RNA profiling by nanopore sequencing with CIRI-long	72
CRISPR–Cas9-mediated chromosome engineering in Arabidopsis thaliana	71
FIND-seq: high-throughput nucleic acid cytometry for rare single-cell transcriptomics	70
Transcriptome-wide measurement of poly(A) tail length and composition at subnanogram total RNA sensitivity by PAIso-seq	70
Biolayer interferometry for measuring the kinetics of protein–protein interactions and nanobody binding	69
High-resolution in situ structure determination by cryo-electron tomography and subtomogram averaging using emClarity	68
Routine culture and study of adult human brain cells from neurosurgical specimens	68
A mouse model of volumetric muscle loss and therapeutic scaffold implantation	68