OOIR: Observatory of International Research

Papers

(The H4-Index of Nature Protocols is 67. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-08-01 to 2025-08-01.)

Article	Citations
Author Correction: Z-REX: shepherding reactive electrophiles to specific proteins expressed tissue specifically or ubiquitously, and recording the resultant functional electrophile-induced redox respo	1091
Author Correction: cfSNV: a software tool for the sensitive detection of somatic mutations from cell-free DNA	534
Open-vessel polymerization of N-carboxyanhydride (NCA) for polypeptide synthesis	406
Open thoracic surgical implantation of cardiac pacemakers in rats	382
Purification and functional characterization of novel human skeletal stem cell lineages	369
Isoform-specific RNA structure determination using Nano-DMS-MaP	328
ChromEMT: visualizing and reconstructing chromatin ultrastructure and 3D organization in situ	316
Routine culture and study of adult human brain cells from neurosurgical specimens	310
Design and application of the transformer base editor in mammalian cells and mice	258
Analysis of combinatorial CRISPR screens with the Orthrus scoring pipeline	245
Visualization of metabolites and microbes at high spatial resolution using MALDI mass spectrometry imaging and in situ fluorescence labeling	234
Generating human bone marrow organoids for disease modeling and drug discovery	220
GLORI for absolute quantification of transcriptome-wide m6A at single-base resolution	219
Generation of heart-forming organoids from human pluripotent stem cells	209
Reproducible strategy for excisional skin-wound-healing studies in mice	204
Quantitative molecular imaging using deep magnetic resonance fingerprinting	178
Expansion and fluctuations-enhanced microscopy for nanoscale molecular profiling of cells and tissues	176
Author Correction: Reliable detection of somatic mutations in solid tissues by laser-capture microdissection and low-input DNA sequencing	174
Head-mounted central venous access during optical recordings and manipulations of neural activity in mice	166
Author Correction: Chromatin integration labeling for mapping DNA-binding proteins and modifications with low input	165
Publisher Correction: Rapid reaction optimization by robust and economical quantitative benchtop 19F NMR spectroscopy	162
How to isolate channel-forming membrane proteins using the E. coli expression system	161
Publisher Correction: In vivo pulse labeling of isochronic cohorts of cells in the central nervous system using FlashTag	159
Fabrication of thin-film electrodes and organic electrochemical transistors for neural implants	154
Precise tracking of nanoparticles in plant roots	149

Preparation and biomedical applications of single-metal atom catalysts	148
Spatially resolved epigenome sequencing via Tn5 transposition and deterministic DNA barcoding in tissue	147
Photoimmunotechnology as a powerful biological tool for molecular-based elimination of target cells and microbes, including bacteria, fungi and viruses	141
Hyperosmolar blood–brain barrier opening using intra-arterial injection of hyperosmotic mannitol in mice under real-time MRI guidance	137
Small RNA structural biochemistry in a post-sequencing era	136
SubCellBarCode: integrated workflow for robust spatial proteomics by mass spectrometry	128
High-throughput fabrication of antimicrobial phage microgels and example applications in food decontamination	128
Generation and characterization of hair-bearing skin organoids from human pluripotent stem cells	127
Stable isotope tracing to assess tumor metabolism in vivo	124
Rapid reaction optimization by robust and economical quantitative benchtop 19F NMR spectroscopy	120
Three-dimensional human neural culture on a chip recapitulating neuroinflammation and neurodegeneration	116
Fabrication of electron tunneling probes for measuring single-protein conductance	113
Tutorial: using nanoneedles for intracellular delivery	105
Chromatin accessibility profiling by ATAC-seq	104
Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds	99
Establishment of human induced trophoblast stem cells via reprogramming of fibroblasts	98
Sequential orthogonal assays for longitudinal and endpoint characterization of three-dimensional spheroids	97
Sonoafterglow nanoprobes for deep-tissue imaging of peroxynitrite	95
Achieving transient and reversible optical transparency in live mice with tartrazine	95
A versatile tissue-rolling technique for spatial-omics analyses of the entire murine gastrointestinal tract	95
Generation and long-term culture of human cerebellar organoids from pluripotent stem cells	95
Gene expression and chromatin state mapped in space	95
Scalable growth of vertical graphene nanosheets by thermal chemical vapor deposition	94
Structural connectome construction using constrained spherical deconvolution in multi-shell diffusion-weighted magnetic resonance imaging	92
Fibrous polyisocyanide hydrogels for 3D cell culture applications	91
Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia	90
mRNA lipid nanoparticle formulation, characterization and evaluation	89
Unraveling cell–cell communication with NicheNet by inferring active ligands from transcriptomics data	83
Large-scale growth of C. elegans and isolation of membrane protein complexes	83
Honey bee functional genomics using symbiont-mediated RNAi	83
Controlling the polarity of human gastrointestinal organoids to investigate epithelial biology and infectious diseases	81
Collections from Nature Protocols	81
Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility	76
Preparation of selective organ-targeting (SORT) lipid nanoparticles (LNPs) using multiple technical methods for tissue-specific mRNA delivery	75
A ‘print–pause–print’ protocol for 3D printing microfluidics using multimaterial stereolithography	75
Comprehensive synthesis of Ti3C2Tx from MAX phase to MXene	74
Preparation and quantitative analysis of multicenter luminescence materials for sensing function	73
Publisher Correction: A reconstituted cell-free assay for the evaluation of the intrinsic activity of purified human ribosomes	72
A bifunctional molecule-assisted synthesis of mimics for use in probing the ubiquitination system	71
Author Correction: Efficient and strand-specific profiling of replicating chromatin with enrichment and sequencing of protein-associated nascent DNA in mammalian cells	71
Integrating genome-wide CRISPR screens and in silico drug profiling for targeted antidote development	70
Identification of immunogenic commensal antigens using phage display	69
Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting	67
Divergent synthesis of amino acid-linked O-GalNAc glycan core structures	67