Nature Protocols

Article	Citations
Author Correction: Z-REX: shepherding reactive electrophiles to specific proteins expressed tissue specifically or ubiquitously, and recording the resultant functional electrophile-induced redox respo	1091
Author Correction: cfSNV: a software tool for the sensitive detection of somatic mutations from cell-free DNA	534
Open-vessel polymerization of N-carboxyanhydride (NCA) for polypeptide synthesis	406
Open thoracic surgical implantation of cardiac pacemakers in rats	382
Purification and functional characterization of novel human skeletal stem cell lineages	369
Isoform-specific RNA structure determination using Nano-DMS-MaP	328
ChromEMT: visualizing and reconstructing chromatin ultrastructure and 3D organization in situ	316
Routine culture and study of adult human brain cells from neurosurgical specimens	310
Design and application of the transformer base editor in mammalian cells and mice	258
Analysis of combinatorial CRISPR screens with the Orthrus scoring pipeline	245
Visualization of metabolites and microbes at high spatial resolution using MALDI mass spectrometry imaging and in situ fluorescence labeling	234
Generating human bone marrow organoids for disease modeling and drug discovery	220
GLORI for absolute quantification of transcriptome-wide m6A at single-base resolution	219
Generation of heart-forming organoids from human pluripotent stem cells	209
Reproducible strategy for excisional skin-wound-healing studies in mice	204
Quantitative molecular imaging using deep magnetic resonance fingerprinting	178
Expansion and fluctuations-enhanced microscopy for nanoscale molecular profiling of cells and tissues	176
Author Correction: Reliable detection of somatic mutations in solid tissues by laser-capture microdissection and low-input DNA sequencing	174
Head-mounted central venous access during optical recordings and manipulations of neural activity in mice	166
Author Correction: Chromatin integration labeling for mapping DNA-binding proteins and modifications with low input	165
Publisher Correction: Rapid reaction optimization by robust and economical quantitative benchtop 19F NMR spectroscopy	162
How to isolate channel-forming membrane proteins using the E. coli expression system	161
Publisher Correction: In vivo pulse labeling of isochronic cohorts of cells in the central nervous system using FlashTag	159
Fabrication of thin-film electrodes and organic electrochemical transistors for neural implants	154
Precise tracking of nanoparticles in plant roots	149

Preparation and biomedical applications of single-metal atom catalysts	148
Spatially resolved epigenome sequencing via Tn5 transposition and deterministic DNA barcoding in tissue	147
Photoimmunotechnology as a powerful biological tool for molecular-based elimination of target cells and microbes, including bacteria, fungi and viruses	141
Hyperosmolar blood–brain barrier opening using intra-arterial injection of hyperosmotic mannitol in mice under real-time MRI guidance	137
Small RNA structural biochemistry in a post-sequencing era	136
High-throughput fabrication of antimicrobial phage microgels and example applications in food decontamination	128
SubCellBarCode: integrated workflow for robust spatial proteomics by mass spectrometry	128
Generation and characterization of hair-bearing skin organoids from human pluripotent stem cells	127
Stable isotope tracing to assess tumor metabolism in vivo	124
Rapid reaction optimization by robust and economical quantitative benchtop 19F NMR spectroscopy	120
Three-dimensional human neural culture on a chip recapitulating neuroinflammation and neurodegeneration	116
Fabrication of electron tunneling probes for measuring single-protein conductance	113
Tutorial: using nanoneedles for intracellular delivery	105
Chromatin accessibility profiling by ATAC-seq	104
Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds	99
Establishment of human induced trophoblast stem cells via reprogramming of fibroblasts	98
Sequential orthogonal assays for longitudinal and endpoint characterization of three-dimensional spheroids	97
Generation and long-term culture of human cerebellar organoids from pluripotent stem cells	95
Gene expression and chromatin state mapped in space	95
Sonoafterglow nanoprobes for deep-tissue imaging of peroxynitrite	95
Achieving transient and reversible optical transparency in live mice with tartrazine	95
A versatile tissue-rolling technique for spatial-omics analyses of the entire murine gastrointestinal tract	95
Scalable growth of vertical graphene nanosheets by thermal chemical vapor deposition	94
Structural connectome construction using constrained spherical deconvolution in multi-shell diffusion-weighted magnetic resonance imaging	92
Fibrous polyisocyanide hydrogels for 3D cell culture applications	91
Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia	90
mRNA lipid nanoparticle formulation, characterization and evaluation	89
Unraveling cell–cell communication with NicheNet by inferring active ligands from transcriptomics data	83
Large-scale growth of C. elegans and isolation of membrane protein complexes	83
Honey bee functional genomics using symbiont-mediated RNAi	83
Controlling the polarity of human gastrointestinal organoids to investigate epithelial biology and infectious diseases	81
Collections from Nature Protocols	81
Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility	76
Preparation of selective organ-targeting (SORT) lipid nanoparticles (LNPs) using multiple technical methods for tissue-specific mRNA delivery	75
A ‘print–pause–print’ protocol for 3D printing microfluidics using multimaterial stereolithography	75
Comprehensive synthesis of Ti3C2Tx from MAX phase to MXene	74
Preparation and quantitative analysis of multicenter luminescence materials for sensing function	73
Publisher Correction: A reconstituted cell-free assay for the evaluation of the intrinsic activity of purified human ribosomes	72
Author Correction: Efficient and strand-specific profiling of replicating chromatin with enrichment and sequencing of protein-associated nascent DNA in mammalian cells	71
A bifunctional molecule-assisted synthesis of mimics for use in probing the ubiquitination system	71
Integrating genome-wide CRISPR screens and in silico drug profiling for targeted antidote development	70
Identification of immunogenic commensal antigens using phage display	69
Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting	67
Divergent synthesis of amino acid-linked O-GalNAc glycan core structures	67
Plant sperm cell sequencing for genome phasing and determination of meiotic crossover points	61
Electrochemical molecular intercalation and exfoliation of solution-processable two-dimensional crystals	60
All-optical interrogation of neural circuits in behaving mice	59
Scalable tissue labeling and clearing of intact human organs	59
CONIPHER: a computational framework for scalable phylogenetic reconstruction with error correction	58
Chemometric analysis in Raman spectroscopy from experimental design to machine learning–based modeling	57

Web-based multi-omics integration using the Analyst software suite	57
High-throughput single-cell transcriptomics of bacteria using combinatorial barcoding	56
Surface nanocoating of bacteria as a versatile platform to develop living therapeutics	56
In vitro reconstitution of COPII vesicles from Arabidopsis thaliana suspension-cultured cells	55
A guide to building a low-cost centrifuge force microscope module for single-molecule force experiments	54
Author Correction: Capture-C: a modular and flexible approach for high-resolution chromosome conformation capture	54
A scalable protocol for the radiosynthesis of clinical grade lutetium-177-labeled theranostic agents	54
Use of synthetic circular RNA spike-ins (SynCRS) for normalization of circular RNA sequencing data	54
Design and simulation of DNA, RNA and hybrid protein–nucleic acid nanostructures with oxView	53
Enzyme-free targeted DNA demethylation using CRISPR–dCas9-based steric hindrance to identify DNA methylation marks causal to altered gene expression	53
Sonication-assisted protein extraction improves proteomic detection of membrane-bound and DNA-binding proteins from tumor tissues	53
Enhancing consistency in brain imaging research for population neuroimaging	51
Genome-wide analysis of the biophysical properties of chromatin and nuclear proteins in living cells with Hi-D	51
A guide to precise measurements of isotope abundance by ESI-Orbitrap MS	50
GlYcoLISA: antigen-specific and subclass-specific IgG Fc glycosylation analysis based on an immunosorbent assay with an LC–MS readout	50
Identification and validation of microbial biomarkers from cross-cohort datasets using xMarkerFinder	50
Comprehensive microcrystal electron diffraction sample preparation for cryo-EM	49
Integrating optical neuroscience tools into touchscreen operant systems	49
Profiling native pulmonary basement membrane stiffness using atomic force microscopy	49
Accurate determination of protein:ligand standard binding free energies from molecular dynamics simulations	48
Seed amplification assay for the detection of pathologic alpha-synuclein aggregates in cerebrospinal fluid	48
Easy and accurate protein structure prediction using ColabFold	47
Capturing acyl–enzyme intermediates with genetically encoded 2,3-diaminopropionic acid for hydrolase substrate identification	47
Construction of complex bacteriogenic protocells from living material assembly	47
Droplet-based functional CRISPR screening of cell–cell interactions by SPEAC-seq	46
High-confidence and high-throughput quantification of synapse engulfment by oligodendrocyte precursor cells	46
Spatial analysis of tissue immunity and vascularity by light sheet fluorescence microscopy	46
A graphic and command line protocol for quick and accurate comparisons of protein and nucleic acid structures with US-align	45
Annotating genetic variants to target genes using H-MAGMA	45
Constructing and interpreting volcano plots and activity maps to navigate homogeneous catalyst landscapes	45
In vivo and ex vivo electrophysiological study of the mouse heart to characterize the cardiac conduction system, including atrial and ventricular vulnerability	45
Outcome devaluation as a method for identifying goal-directed behaviors in rats	45
CellPhoneDB v5: inferring cell–cell communication from single-cell multiomics data	44
An optimized culture system for efficient derivation of porcine expanded potential stem cells from preimplantation embryos and by reprogramming somatic cells	44
Preparation and use of wireless reprogrammable multilateral optogenetic devices for behavioral neuroscience	44
Optimal 13C NMR investigation of intrinsically disordered proteins at 1.2 GHz	44
Analyzing somatic mutations by single-cell whole-genome sequencing	43
Applications of and considerations for using CRISPR–Cas9-mediated gene conversion systems in rodents	43
Author Correction: PepSeq: a fully in vitro platform for highly multiplexed serology using customizable DNA-barcoded peptide libraries	43
Stabilization and structure determination of integral membrane proteins by termini restraining	42
CRISPR-Combo–mediated orthogonal genome editing and transcriptional activation for plant breeding	42
RNA sample optimization for cryo-EM analysis	41
High-speed laser-scanning biological microscopy using FACED	40
Standardized production of hPSC-derived cardiomyocyte aggregates in stirred spinner flasks	40
Printed photonic crystal biochips for rapid and sensitive detection of biomarkers in various body fluids	39
Capture of the newly transcribed RNA interactome using click chemistry	39
Circular Vectors as an efficient, fully synthetic, cell-free approach for preparing small circular DNA as a plasmid substitute for guide RNA expression in CRISPR–Cas9 genome editing	38
Surface photovoltage microscopy for mapping charge separation on photocatalyst particles	38
High-resolution in situ structure determination by cryo-electron tomography and subtomogram averaging using emClarity	38
Physical dynamic double-network hydrogels as dressings to facilitate tissue repair	37
Fabrication of liquid cell for in situ transmission electron microscopy of electrochemical processes	37
Addendum: Micro-CT acquisition and image processing to track and characterize pulmonary nodules in mice	37
Preparation of near-infrared AIEgen-active fluorescent probes for mapping amyloid-β plaques in brain tissues and living mice	37
Biomarker analysis from complex biofluids by an on-chip chemically modified light-controlled vertical nanopillar array device	36
Author Correction: Production and use of antigen tetramers to study antigen-specific B cells	36
Improving accuracy and reproducibility of mass spectrometry characterization of protein coronas on nanoparticles	35
Synthesis of N-heterocycles through alcohol dehydrogenative coupling	35
Direct analysis of biotransformations with mass spectrometry—DiBT-MS	35
Simultaneous ribosome profiling of hundreds of microbes from the human microbiome	35
YCharOS protocol for antibody validation	35
Spatially resolved genome-wide joint profiling of epigenome and transcriptome with spatial-ATAC-RNA-seq and spatial-CUT&Tag-RNA-seq	35
Mapping T cell dynamics to molecular profiles through behavior-guided transcriptomics	34
High-throughput multiplex voltage-clamp/current-clamp evaluation of acutely isolated neurons	34
Activation of mechanoluminescent nanotransducers by focused ultrasound enables light delivery to deep-seated tissue in vivo	33
m6A-SAC-seq for quantitative whole transcriptome m6A profiling	33
Co-fractionation–mass spectrometry to characterize native mitochondrial protein assemblies in mammalian neurons and brain	33
Visualizing epigenetic modifications and their spatial proximities in single cells using three DNA-encoded amplifying FISH imaging strategies: BEA-FISH, PPDA-FISH and Cell-TALKING	33
A detailed guide to assessing genome assembly based on long-read sequencing data using Inspector	31
A multi-glycomic platform for the analysis of food carbohydrates	31
DARLIN mouse for in vivo lineage tracing at high efficiency and clonal diversity	31
Purification of mammalian telomeric DNA for single-molecule analysis	30
Luciferase- and HaloTag-based reporter assays to measure small-molecule-induced degradation pathway in living cells	30
Tutorial: a guide to diffusion MRI and structural connectomics	30
Nano3P-seq: charting the coding and noncoding transcriptome at single-molecule resolution	30
Generation of ‘semi-guided’ cortical organoids with complex neural oscillations	30
A consensus platform for antibody characterization	30
Universal platform for the generation of thermostabilized GPCRs that crystallize in LCP	29
Determining chromatin architecture with Micro Capture-C	29
Graphene–molecule–graphene single-molecule junctions to detect electronic reactions at the molecular scale	28
A proposed framework to evaluate the quality and reliability of targeted metabolomics assays from the UK Consortium on Metabolic Phenotyping (MAP/UK)	28

The HADDOCK2.4 web server for integrative modeling of biomolecular complexes	28
A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys	28
Fabrication and use of silicon hollow-needle arrays to achieve tissue nanotransfection in mouse tissue in vivo	28
Protocols and risks: when less is more	28
A new era begins at Nature Protocols	27
Preparation, maintenance and propagation of synchronous cultures of photoactive Chlamydomonas cells	27
Photothermal nanofiber-mediated photoporation for gentle and efficient intracellular delivery of macromolecules	27
High spatial and temporal resolution Ca2+ imaging of myocardial strips from human, pig and rat	27