Biotechnology Journal

Article	Citations
Biomedical application of microalgal‐biomaterials hybrid system	44
Potential of butanol production from Thailand marine macroalgae using Clostridium beijerinckii ATCC 10132‐based ABE fermentation	40
Feeding strategies for Sporosarcina pasteurii cultivation unlock more efficient production of ureolytic biomass for MICP	34
Redesigning glycolaldehyde synthase for the synthesis of biorefinery feedstock D‐xylulose from C1 compounds	34
Scaling strategy for cell and gene therapy bioreactors based on turbulent parameters	33
Design and evaluation of a 3D‐printed, lab‐scale perfusion bioreactor for novel biotechnological applications	32
Overexpression of Geranylgeranyl Diphosphate Synthase and Cyclase Enhances Pleuromutilin Production in Clitopilus Passeckerianus T6	32
	31
Engineering of the start condensation domain with improved N‐decanoyl catalytic activity for daptomycin biosynthesis	31
Multifunctional PAMAM Dendrimers Carrying SAHA, 5‐FU, and a Therapeutic Gene for Targeted Co‐Delivery Toward Colorectal Cancer Cells	31
Site specific insertion of a transgene into the murine α‐casein (CSN1S1) gene results in the predictable expression of a recombinant protein in milk	31
Issue Information: Biotechnology Journal 9/2025	30
Dialysis rolled scaffold bioreactor allows extended production of monoclonal antibody with reduced media use	28
3D Printed Microfluidic Chromatographic Column for Fast Downstream Processing Development	28
Expression and application of aflatoxin degrading enzyme gene in Pichia pastoris	27
Rational Design of an Epoxide Hydrolase From Spatholobus Suberectus: Enhancing Catalytic Activity and Thermostability for Efficient (R)‐Styrene Oxide Production	26
Live‐cell imaging of genomic loci with Cas9 variants	26
Intermediate product control in cascade reaction for one‐pot production of ε‐caprolactone by Escherichia coli	26
Toehold switch plus signal amplification enables rapid detection	25
Improved synthesis of dietary triglycerides by using lipase supported on clay carriers	25
Cell fate‐inducing CARs orthogonally control multiple signaling pathways	23
Osteochondral Tissue Engineering: Scaffold Materials, Fabrication Techniques and Applications	23
Numerical Simulation and Comparison of Flow Field in Different Dynamic Co‐Culture Conditions	23
Multiomics approaches and genetic engineering of metabolism for improved biorefinery and wastewater treatment in microalgae	23
Reconstructing an Inducible Homologous Recombination System in Ogataea Polymorpha	22

A generic pump‐free organ‐on‐a‐chip platform for assessment of intestinal drug absorption	22
Engineering a 3‐dimensional tissue construct with adipose‐derived stem cells for healing bone defect: An ex vivo study with femur head	21
Microalgae‐made human vaccines and therapeutics: A decade of advances	20
Through virtual saturation mutagenesis and rational design for superior substrate conversion in engineered d‐amino acid oxidase	20
	20
A new look at the fluorescent protein‐based approach for identifying optimal coding sequence for recombinant protein expression in E. coli	20
The evaluation of biotechnological potential of Gp144, the key molecule of natural predator bacteriophage K in Staphylococcus aureus hunting mechanism	19
Editorial Board: Biotechnology Journal 12/2021	19
Massively parallel DNA target capture using long adapter single stranded oligonucleotide (LASSO) probes assembled through a novel DNA recombinase mediated methodology	19
Optimization of Isoamylase Production in Escherichia coli Under Different Nutrient Limitation Conditions	19
Metabolic engineering of Aspergillus niger for accelerated malic acid biosynthesis by improving NADPH availability	19
Enhancing production and purity of 9‐OH‐AD from phytosterols by balancing metabolic flux of the side‐chain degradation and 9‐position hydroxylation in Mycobacterium neoaurum	19
In vitro acne disease model from inertial focusing effect for studying the interactions between sebocyte glands and macrophages	19
Bio‐based polymers containing traditional medicinal fillers for wound healing applications – An evaluation of neoteric development and future perspectives	19
Outside Front Cover: (Biotechnology Journal 10/2022)	19
Comparison of protein quantification methods for protein encapsulation with ZIF‐8 metal‐organic frameworks	19
Characterization and discrimination of spike protein in SARS‐CoV‐2 virus‐like particles via surface‐enhanced Raman spectroscopy	18
Fabrication of a decellularized liver matrix–based hepatic patch for the repair of CCl4‐induced liver injury	18
Cephalotaxus‐Derived Homoharringtonine: Biosynthesis, Mechanism of Action, and Role in Modern Cancer Therapy	18
A Competition‐Based Strategy for the Isolation of an Anti‐Idiotypic Blocking Module and Fine‐Tuning for Conditional Activation of a Therapeutic Antibody	18
Rapid At‐Line AAVX Affinity HPLC: Enabling Process Analytical Technology for Bioprocess Development of Adeno‐Associated Virus Vectors	18
Multiomics analysis reveals the biological effects of live Roseburia intestinalis as a high‐butyrate‐producing bacterium in human intestinal epithelial cells	18
Microbial Biosynthesis of Natural Esters via Enzyme and Metabolic Engineering	17
Synthetic Osmolytes for Enhanced RNA Thermotolerance and Extended Plasmid Storage at Room Temperature	17
Single‐particle fluorescence tracking combined with TrackMate assay reveals highly heterogeneous and discontinuous lysosomal transport in freely orientated axons	17
A high‐fidelity, dual site‐specific integration system in CHO cells by a Bxb1 recombinase	17
Direct capture and selective elution of a secreted polyglutamate‐tagged nanobody using bare magnetic nanoparticles	17
Mimicking continuous capture chromatography for virus clearance using a single chromatography column model	17
Combination of MAR and intron increase transgene expression of episomal vectors in CHO cells	17
Effect of High‐Speed Shaking on Oxygen Transfer in Shake Flasks	17
Editorial Board: Biotechnology Journal 3/2022	17
Cas9‐orthologue‐mediated cytosine and adenine base editors recognizing NNAAAA PAM sequences	16
Dopaminergic signaling regulates zebrafish larvae's response to electricity	16
	16
Single‐cell genotyping of phytoplankton from ocean water by gel‐based cell manipulation	16
DNA origami scaffold promoting nerve guidance and regeneration	16
Adsorption and Separation of the Biomolecular Motors of FOF1‐ATPase‐Embedded Chromatophores Using Titanium Dioxide Microsphere	16
Synthetic Genetic Circuits Enabled in Komagataella phaffii Through T7 RNAP/CRISPRa System	16
Promoter screening and identification for metabolic regulation in Acremonium chrysogenum	15
Improving bioluminescence of a minimal luciferase by adding a charged oligopeptide: picALuc2.0	15
Non‐invasive and time‐resolved measurement of the respiration activity of Chinese hamster ovary cells enables prediction of key culture parameters in shake flasks	15
A single‐cell genomic strategy for alternative transcript start sites identification	15
Issue Information: Biotechnology Journal 7/2025	14
Highly efficient identification of nucleocytoplasmic O‐glycosylation by the TurboID‐based proximity labeling method in living cells	14
Intracellular Antibody Levels Predict Secretion Efficiency in HEK293T Cells Expressing a Chimeric Anti‐CD99 Antibody from a Single‐Expression Plasmid	14
Neuroprotective Potential of Human Platelet Lysate in Parkinson's Disease: Insights Into Oxidative Stress, Mitochondrial Dysfunction, Cell Death, and Reactive Gliosis in Experimental Models	14
Biomass specific perfusion rate as a control lever for the continuous manufacturing of biosimilar monoclonal antibodies from CHO cell cultures	14
Enhanced synthesis of S‐adenosyl‐L‐methionine through combinatorial metabolic engineering and Bayesian optimization in Saccharomyces cerevisiae	14
Magnetic resonance imaging as a tool for quality control in extrusion‐based bioprinting	13
Lipofection with Lipofectamine™ 2000 in a heparin‐free growth medium results in high transfection efficiency in chicken primordial germ cells	13

Enhanced targeting: Production of filamentous bacteriophage monoclonal antibodies using the major coat protein pVIII as anchor	13
Editorial Board: Biotechnology Journal 1/2023	13
Investigation of the Effects of Applying Bio‐Magnesium Oxide Nanoparticle Fertilizer to Moringa Oleifera Plants on the Chemical and Vegetative Properties of the Plants’ leaves	13
	13
Dinucleotide tag‐based parallel reporter gene assay method enables efficient identification of regulatory mutations	13
Issue Information: Biotechnology Journal 12/2024	13
	13
Metabolic engineering of threonine catabolism enables Saccharomyces cerevisiae to produce propionate under aerobic conditions	12
Editorial Board: Biotechnology Journal 1/2024	12
Advances in ε‐Poly‐Lysine Biosynthesis, Selection of High‐Yielding Strains and Regulatory Mechanisms	12
Exosomal lncRNA USP30‐AS1 activates the Wnt/β‐catenin signaling pathway to promote cervical cancer progression via stabilization of β‐catenin by USP30	12
Dissecting cell death pathways in fed‐batch bioreactors	12
Enhancing the expression of terminal deoxynucleotidyl transferases by N‐terminal truncation	12
Continuous phase separation of stable emulsions from biphasic whole‐cell biocatalysis by catastrophic phase inversion	12
Outside Front Cover: (Biotechnology Journal 5/2023)	12
Expanding the targeting scope of FokI‐dCas nuclease systems with SpRY and Mb2Cas12a	12
Proteomic workflows for deep phenotypic profiling of 3D organotypic liver models	12
Development of a biotechnology process for the production of a novel hyperglycosylated long‐acting recombinant bovine follicle‐stimulating hormone	12
Preparation of Thermosensitive Lignocellulose Hollow Fiber Membrane Grafted With PNIPAAm and Its Application as a Cell Culture Carrier in a RSOC Dynamic Culture	12
Outside Front Cover: (Biotechnology Journal 6/2023)	12
Improved adeno‐associated virus empty and full capsid separation using weak partitioning multi‐column AEX chromatography	12
Lipidomic and transcriptomic analysis of the increase in eicosapentaenoic acid under cobalamin deficiency of Schizochytrium sp	11
Microbial and Enzymatic Biodegradation of Polyurethane: From Depolymerization to Monomer Valorization	11
Development and optimization of Lysis gene E as a counter‐selection marker with high stringency	11
Recombinant human fibroblast growth factor 7 obtained from stable Chinese hamster ovary cells enhances wound healing	11
Successful reversal of transgene silencing in Chlamydomonas reinhardtii	11
A machine learning‐based approach for improving plasmid DNA production in Escherichia coli fed‐batch fermentations	11
Expanding the Spectrum: Modified Bacteriocin Nisin as a Trojan Horse Against Gram‐Negative Salmonella Typhimurium and Escherichia coli	11
Enhancement of Recombinant Adeno‐Associated Virus Production in HEK293 Cells by Application of Small Molecule Additives and Genetic Regulation Based on Perfusion Technique	11
Toward standardized solid medium cultivations: Online microbial monitoring based on respiration activity	11
Optimizing Lentiviral Vector Production: Insights Into PiggyBac Transposase and Concatemeric Array Strategies	11
YBac: A novel and simple baculovirus system for heterologous protein production in Spodoptera frugiperda 9 (Sf9) cells	11
Chromatin Accessibility Plays an Important Epigenetic Role on Antibody Expression From CMV Promoter and DNA Elements Flanking the CHO TI Host Landing‐Pad	11
The economic value of genetically engineered mosquitoes as a malaria control strategy depends on local transmission rates	11
	10
Liamocins overproduction via the two‐pH stage fermentation and anti‐Aspergillus flavus activity of Massoia lactone	10
Hierarchical time‐series analysis of dynamic bioprocess systems	10
Metabolic Engineering of Komagataella phaffii and Process Optimization for Biosynthesis of 1,2,4‐Butanetriol From Xylose	10
Autofluorescence prediction model for fluorescence unmixing and age determination	10
Bioinspired 3D microprinted cell scaffolds: Integration of graph theory to recapitulate complex network wiring in lymph nodes	10
Clioquinol rescues yeast cells from Aβ42 toxicity via the inhibition of oxidative damage	10
3D‐QSAR pharmacophore modeling, virtual screening, molecular docking, MD simulations, in vitro and in vivo studies to identify potential anti‐hyperplasia drugs	10
Editorial Board: Biotechnology Journal 6/2022	10
Development of an efficient liposomal DOX delivery formulation for HCC therapy by targeting CK2α	10
mRNA Vaccines: Design Principles, Mechanisms, and Manufacturing—Insights From COVID‐19 as a Model for Combating Infectious Diseases	10
Semi‐rational engineering of D‐allulose 3‐epimerase for simultaneously improving the catalytic activity and thermostability based on D‐allulose biosensor	10
Exploring nutrient supplementation and bioprocess optimization to improve the production of lentiviral vectors in serum‐free medium suspension cultures	10
Efficient production of 22(R)‐hydroxycholesterol via combination optimization of Saccharomyces cerevisiae	10
Chicken‐Derived Single‐Chain Variable Fragments Targeting Monkeypox Virus A29L Protein	10
Octadecyl and sulfonyl modification of diatomite synergistically improved the immobilization efficiency of lipase and its application in the synthesis of pine sterol esters	10
Advancing Protein‐Coding RNA Engineering: From Structural Refinement to Biomedical Implementation	10
The Mechanism Underlying Enhanced Coal‐to‐Methane Conversion in Anaerobic Digestion With Betaine Supplementation	10
Enhancing Carbon Metabolic Flow Ability for Titer Improvement of L‐Lactic Acid in Bacillus coagulans Through Heavy Ion Mutagenesis	10
Simultaneous production of 2′‐fucosyllactose and difucosyllactose by engineered Escherichia coli with high secretion efficiency	10
Development, optimization, and scale‐up of suspension Vero cell culture process for high titer production of oncolytic herpes simplex virus‐1	10
A 3D paper microfluidic device for enzyme‐linked assays: Application to DNA analysis	10
Deglycosylation of VEGF Produced in Pichia pastoris Reduces its in Vitro Binding and Angiogenic Effect	10
Gulo gene locus, a new gene editing locus for mammalian cells	10
Comprehensive mRNA‐sequencing‐based characterization of three HEK‐293 cell lines during an rAAV production process for gene therapy applications	10
Construction of a Cell Factory for the Targeted and Efficient Production of Phytosterol to Boldenone in Mycobacterium neoaurum	10
In vitro and in silico analyses reveal the interaction between LysM receptor‐like kinase3 of Solanum tuberosum and the carbohydrate elicitor Riclin octaose	9
Model‐driven design of synthetic N‐terminal coding sequences for regulating gene expression in yeast and bacteria	9
Impact of Eco‐Friendly Surfactant Structure and Class on Enveloped Virus Inactivation	9
	9
Evaluation of expression systems for recombinant protein production in chicken egg bioreactors	9
Electrochemical Glucose Sensors: Classification, Catalyst Innovation, and Sampling Mode Evolution	9
Generation of reproductive transgenic pigs of a CRISPR‐Cas9‐based oncogene‐inducible system by somatic cell nuclear transfer	9
Integrating Biofilm‐Based Fermentation With Continuous Processes for Improved L‐Valine Production	9
Initial cyclic‐di‐GMP upregulation triggers sporadic cellular expansion leading to improved cellular survival	9
Amino acid degradation pathway inhibitory by‐products trigger apoptosis in CHO cells	9
1H NMR‐based process understanding and biochemical marker identification methodology for monitoring CHO cell culture process during commercial‐scale manufacturing	9
Promoting cell growth and characterizing partial symbiotic relationships in the co‐cultivation of green alga Chlamydomonas reinhardtii and Escherichia coli	9
Molecular design of controllable recombinant adeno‐associated virus (AAV) expression systems for enhanced vector production	9
Antibacterial functionalization of calfskin bioremediation film	9
A 3D bioprinted antibacterial hydrogel dressing of gelatin/sodium alginate loaded with ciprofloxacin hydrochloride	9
Design of a microfluidic mixer channel: First steps into creating a fluorescent dye‐based biosensor for mAb aggregate detection	9
Secretory production of 7‐dehydrocholesterol by engineered Saccharomyces cerevisiae	9
Editorial Board: Biotechnology Journal 4/2022	9
In vitro evolution of diagnostic antibodies targeting native antigens in plasma by sandwich flow cytometry	9
Uncovering Growth‐Inhibitory Metabolic Byproducts in HEK293 Fed‐Batch Cultures and Strategies for Their Control	9

Efficient production of glutathione in Saccharomyces cerevisiae via a synthetic isozyme system	9
Characterization of the Ubiquitin‐Modified Proteome of Recombinant Chinese Hamster Ovary Cells in Response to Endoplasmic Reticulum Stress	9
	9
A single‐cell surgery microfluidic device for transplanting tumor cytoplasm into dendritic cells without nuclei mixing	9
Advances in Miniaturized Bioreactors: Bridging Biotechnology and Tissue Engineering for Enhanced Drug Development	9
Proteomic analysis reveals that the co‐ordination of cytosolic and mitochondrial pathways is beneficial for sabinene biosynthesis in engineered Saccharomyces cerevisiae	9
Recent advances and fundamentals of microalgae cultivation technology	9
Identification of critical process parameters for expansion of clinical grade human Wharton's jelly‐derived mesenchymal stromal cells in stirred‐tank bioreactors	9
Liamocin biosynthesis is induced by an autogenous host acid activation in Aureobasidium melanogenum	9
Outside Front Cover: (Biotechnology Journal 12/2022)	8
Arg128*‐Mediated Dual‐Substrate Recognition and Dynamic Transport Mechanisms in (R)‐ω‐Transaminase: Computational Insights and Mutational Profiling Guided Rational Engineering	8
Expanding the toolbox of probiotic Escherichia coli Nissle 1917 for synthetic biology	8
Generation and Biological Characterization of an Anti‐IL‐6Rα Biosimilar Candidate Antibody	8
Deletion analysis of Pichia pastoris alcohol dehydrogenase 2 (ADH2) promoter and development of synthetic promoters	8
Exiguobacterium mexicanum PY14 Reduces Toxic Selenite to Elemental Selenium: Characterization and Mechanism	8
Transcriptional Downregulation of Methanol Metabolism Key Genes During Yeast Death in Engineered Pichia pastoris	8
Chromosomal Integration of budAB Operons and Pathway Rewiring Enhance Acetoin Production From Starch in Vibrio diabolicus	8
Rational Design of a Novel DNA Polymerase From Clostridium thermocellum to Improve LAMP Detection Performance	8
Microfabricated dynamic brain organoid cocultures to assess the effects of surface geometry on assembloid formation	8
Experimental Research on the Treatment of Saline‐Alkali Diseases of Murals in Arzhai Grottoes With Salt‐Tolerant Mineralizing Bacteria	8
Improvement of the Antimicrobial Peptide Activity by Means of Chitosan‐Based Nanoparticles	8
In situ formation of alginic acid‐gold nanohybrids for application in cancer photothermal therapy	8
	8
CRISPRa/i with Adaptive Single Guide Assisted Regulation DNA (ASGARD) mediated control of Chlorella sorokiniana to enhance lipid and protein production	8
The Experimental Study of Double‐Layer Heterogeneous CA Scaffold in Promoting the Surface Shape Recovery and Internal Osteogenesis of Alveolar Bone	8
Ultrasound assisted in situ separation of sophorolipids in multi‐phase fermentation system to achieve efficient production by Candida bombicola	8
Use of tRNA gene barriers improves stability of transgene expression in CHO cells	8
Resolving the Trade‐Off Between Toxicity and Efficiency of CRISPR‐Cas9 System for Genome Editing Within Escherichia coli	8
Outside Front Cover: (Biotechnology Journal 10/2023)	8
	8
Immune Response of Zika prM Peptide PEG Conjugates in Peripheral Blood Mononuclear Cells	8
Biochemical and Metabolic Engineering of Saccharomyces cerevisiae for the Biosynthesis of Olivetolic Acid, a Key Cannabinoid Precursor	8
Implementation of RAGATH RNA‐associated DNA Endonucleases as Genome Editing Tool in Escherichia coli	8
Drug‐loaded lipid nanoparticles improve the removal rates of the Staphylococcus aureus biofilm	8
Single‐strand annealing: Molecular mechanisms and potential applications in CRISPR‐Cas‐based precision genome editing	8
Concentration‐Dependent Effects of MXene Nanocomposite‐Loaded Carboxymethyl Cellulose on Wound Healing	8
Aptabinding of tumor necrosis factor‐α (TNFα) inhibits its proinflammatory effects and alleviates islet inflammation	8
Biosynthesis of the antioxidant γ‐glutamyl‐cysteine with engineered Yarrowia lipolytica	8
Dual‐layered hydrogels allow complete genome recovery with nucleic acid cytometry	8
Efficient Agrobacterium ‐Mediated Transformation of Green Arabidopsis Suspension Cells	8
Phage display selection of a Pb(II) specific peptide and its application as a biorecognition unit for colorimetric detection of Pb(II) ions	8
Tuning mobile phase properties to improve empty and full particle separation in adeno‐associated virus productions by anion exchange chromatography	8
	7
Engineering phenylalanine ammonia lyase to limit feedback inhibition by cinnamate and enhance biotransformation	7
The immune system of prokaryotes: potential applications and implications for gene editing	7
Computational evaluation of light propagation in cylindrical bioreactors for optogenetic mammalian cell cultures	7
Advancements in Plant Gene Editing Technology: From Construct Design to Enhanced Transformation Efficiency	7
Adaptabodies: Retargeting a Predefined Monoclonal Antibody With Bispecific Nanobodies for Antibody Therapy	7
Light‐induced fermenter production of derivatives of the sweet protein monellin is maximized in prestationary Saccharomyces cerevisiae cultures	7
Microwell‐based flow culture increases viability and restores drug response in prostate cancer spheroids	7
Impact of ethanol on continuous inline diafiltration of liposomal drug products	7
Medical Ultrasound Application Beyond Diagnosis: Insights From Ultrasound Sensing and Biological Response	7
Mitochondrial Oxidative Stress Related Diagnostic Model Accurately Assesses Rheumatoid Arthritis Risk Stratification and Immune Infiltration Characterization	7
Structure‐based discovery of small molecules improving stability of human broadly‐neutralizing anti‐HIV antibody 2F5 in plant suspension cells	7
Rapid construction of Escherichia coli chassis with genome multi‐position integration of isopentenol utilization pathway for efficient and stable terpenoid accumulation	7
Uncertainty quantification for gene delivery methods: A roadmap for pDNA manufacturing from phase I clinical trials to commercialization	7
Study and Application of Microbial Community in Biodegradation of Kitchen Waste Using High‐Throughput Sequencing and Strain Isolation Techniques	7
Issue Information: Biotechnology Journal 7/2024	7
Advances in biosynthesis of peptide drugs: Technology and industrialization	7
Characterisation of the E. coli HMS174 and BLR host cell proteome to guide purification process development	7
Building a machine‐learning model to predict optimal mevalonate pathway gene expression levels for efficient production of a carotenoid in yeast	7
	7
Lung‐specific exosomes for doxorubicin delivery in lung adenocarcinoma therapy	7
Suspension culture promoted the expansion of NK‐92 cells ex vivo by enhancing the expression of IL‐2 receptor	7
	7
Expanding the upper‐temperature boundary for the microalga Picochlorum sp. (BPE23) by adaptive laboratory evolution	7
UV Mutagenesis Enhances DHA Biosynthesis in Schizochytrium sp. via Metabolic Reprogramming	7
Development of a novel microplate for high‐throughput screening and optimization of DHA producing strains based on CFD technology	7
Towards a Platform Chromatography Purification Process for Adeno‐Associated Virus (AAV)	7
Application of Nano‐Drug Delivery Systems in Lung Cancer Diagnosis and Treatment	7
Methods for addressing host cell protein impurities in biopharmaceutical product development	7
Self‐Produced Brain‐Like ECM From 3D‐Cultured Dermal Fibroblasts Enhances Neuronal Growth and Survival	7