Biotechnology Journal

Article	Citations
Quantum Dots: A Review from Concept to Clinic	109
Metal–Organic Framework Composites for Theragnostics and Drug Delivery Applications	102
Mesenchymal Stem Cell‐Derived Exosomes for Effective Cartilage Tissue Repair and Treatment of Osteoarthritis	93
Hydrogel‐Based 3D Bioprinting for Bone and Cartilage Tissue Engineering	91
Pyrazines: Synthesis and Industrial Application of these Valuable Flavor and Fragrance Compounds	86
Green and ecofriendly biosynthesis of selenium nanoparticles using Urtica dioica (stinging nettle) leaf extract: Antimicrobial and anticancer activity	84
Progress and Concept for COVID‐19 Vaccine Development	72
Biohydrogen production from microalgae—Major bottlenecks and future research perspectives	71
Engineering Native and Synthetic Pathways in Pseudomonas putida for the Production of Tailored Polyhydroxyalkanoates	68
Spatial transcriptomics and proteomics technologies for deconvoluting the tumor microenvironment	66
Surface engineering of biomaterials in orthopedic and dental implants: Strategies to improve osteointegration, bacteriostatic and bactericidal activities	66
Advances in Cell‐Free Biosensors: Principle, Mechanism, and Applications	58
Smart Nanocarriers for the Delivery of Nucleic Acid‐Based Therapeutics: A Comprehensive Review	58
Biocompatibility of Biomaterials for Tissue Regeneration or Replacement	57
Production, Processing, and Characterization of Synthetic AAV Gene Therapy Vectors	54
Anything but Conventional Chromatography Approaches in Bioseparation	50
Quality Control Testing, Characterization and Critical Quality Attributes of Adeno‐Associated Virus Vectors Used for Human Gene Therapy	49
Inorganic Porous Nanoparticles for Drug Delivery in Antitumoral Therapy	46
Historical evolution of spheroids and organoids, and possibilities of use in life sciences and medicine	46
Advances in promoter engineering: Novel applications and predefined transcriptional control	46
Pseudomonas as Versatile Aromatics Cell Factory	42
Recent Advances on Graphene Quantum Dots as Multifunctional Nanoplatforms for Cancer Treatment	41
Transporter Engineering for Microbial Manufacturing	40
Progress and Perspectives in the Development of Lentiviral Vector Producer Cells	39
Separating Empty and Full Recombinant Adeno‐Associated Virus Particles Using Isocratic Anion Exchange Chromatography	37

Hybrid Modeling and Intensified DoE: An Approach to Accelerate Upstream Process Characterization	36
Oligonucleotides: Current Trends and Innovative Applications in the Synthesis, Characterization, and Purification	35
Metabolic engineering of threonine catabolism enables Saccharomyces cerevisiae to produce propionate under aerobic conditions	35
Advances in Lentivirus Purification	35
Production of Substituted Styrene Bioproducts from Lignin and Lignocellulose Using Engineered Pseudomonas putida KT2440	32
Recent Advances in the Construction of Flexible Sensors for Biomedical Applications	30
Microbial Electrosynthesis—An Inventory on Technology Readiness Level and Performance of Different Process Variants	30
Engineering Tobacco Mosaic Virus and Its Virus‐Like‐Particles for Synthesis of Biotemplated Nanomaterials	30
Emerging Technologies for the Electrochemical Detection of Bacteria	30
Engineering the oleaginous yeast Yarrowia lipolytica for β‐farnesene overproduction	29
Detection of the SARS‐CoV‐2 D614G mutation using engineered Cas12a guide RNA	29
Generation of enzymatically competent SARS‐CoV‐2 decoy receptor ACE2‐Fc in glycoengineered Nicotiana benthamiana	28
Advancements in molecular design and bioprocessing of recombinant adeno‐associated virus gene delivery vectors using the insect‐cell baculovirus expression platform	28
Modeling the Residence Time Distribution of Integrated Continuous Bioprocesses	25
Xylose Assimilation for the Efficient Production of Biofuels and Chemicals by Engineered Saccharomyces cerevisiae	25
Doxorubicin‐Loaded Physalis Mottle Virus Particles Function as a pH‐Responsive Prodrug Enabling Cancer Therapy	24
Production of MSTN‐mutated cattle without exogenous gene integration using CRISPR‐Cas9	24
Dissolvable Gelatin‐Based Microcarriers Generated through Droplet Microfluidics for Expansion and Culture of Mesenchymal Stromal Cells	23
Self‐Assembled Nanoparticles: Exciting Platforms for Vaccination	23
Evaluation of the Single‐Use Fixed‐Bed Bioreactors in Scalable Virus Production	23
The SCIFF‐Derived Ranthipeptides Participate in Quorum Sensing in Solventogenic Clostridia	21
Antimicrobial and antiviral activities with molecular docking study of chitosan/carrageenan@clove oil beads	21
Demonstrating the Manufacture of Human CAR‐T Cells in an Automated Stirred‐Tank Bioreactor	21
Synergisms of machine learning and constraint‐based modeling of metabolism for analysis and optimization of fermentation parameters	19
Intensified Downstream Processing of Monoclonal Antibodies Using Membrane Technology	19
Real‐time amino acid and glucose monitoring system for the automatic control of nutrient feeding in CHO cell culture using Raman spectroscopy	19
Engineering Acetobacterium woodii for the production of isopropanol and acetone from carbon dioxide and hydrogen	18
Dimethyloxalylglycine, a small molecule, synergistically increases the homing and angiogenic properties of human mesenchymal stromal cells when cultured as 3D spheroids	18
Preclinical Evaluation of a Novel TALEN Targeting CCR5 Confirms Efficacy and Safety in Conferring Resistance to HIV‐1 Infection	18
Hydroxypropyl methylcellulose/graphene oxide composite as drug carrier system for 5‐fluorouracil	18
Influence of biosynthesized magnesium oxide nanoparticles on growth and physiological aspects of cowpea (Vigna unguiculata L.) plant, cowpea beetle, and cytotoxicity	18
Fluorescent Biosensor of CDK5 Kinase Activity in Glioblastoma Cell Extracts and Living Cells	18
Lyophilization stabilizes clinical‐stage core‐crosslinked polymeric micelles to overcome cold chain supply challenges	17
Engineering the Gut Microbiome for Treatment of Obesity: A Review of Current Understanding and Progress	17
Three‐dimensional‐poly(lactic acid) scaffolds coated with gelatin/magnesium‐doped nano‐hydroxyapatite for bone tissue engineering	17
Optimization of muscle cell culture media using nonlinear design of experiments	17
Identification and analysis of sugar transporters capable of co‐transporting glucose and xylose simultaneously	16
13C Metabolic Flux Analysis of Escherichia coli Engineered for Gamma‐Aminobutyrate Production	16
Naming CHO cells for bio‐manufacturing: Genome plasticity and variant phenotypes of cell populations in bioreactors question the relevance of old names	16
L‐malic acid production from xylose by engineered Saccharomyces cerevisiae	16
Transient ammonia stress on Chinese hamster ovary (CHO) cells yield alterations to alanine metabolism and IgG glycosylation profiles	16
Isolation of Common Light Chain Antibodies from Immunized Chickens Using Yeast Biopanning and Fluorescence‐Activated Cell Sorting	16
Rational Engineering of a Multi‐Step Biocatalytic Cascade for the Conversion of Cyclohexane to Polycaprolactone Monomers in Pseudomonas taiwanensis	16
A deep learning approach to evaluate the feasibility of enzymatic reactions generated by retrobiosynthesis	16
Polymersomes as Nanoreactors Enabling the Application of Solvent‐Sensitive Enzymes in Different Biphasic Reaction Setups	15
Biotin Functionalized Self‐Assembled Peptide Nanofiber as an Adjuvant for Immunomodulatory Response	15
A Novel Wick‐Like Paper‐Based Microfluidic Device for 3D Cell Culture and Anti‐Cancer Drugs Screening	15
Micropipette Aspiration‐Based Assessment of Single Channel Water Permeability	15
Factors and methods to modulate DNA hybridization kinetics	15
Optimization of Single‐Column Batch and Multicolumn Continuous Protein A Chromatography and Performance Comparison Based on Mechanistic Model	15

Engineered Live Biotherapeutics: Progress and Challenges	14
Short‐chain alcohols inactivate an immobilized industrial lipase through two different mechanisms	14
An integrative multiomics approach to characterize anti‐adipogenic and anti‐lipogenic effects of Akkermansia muciniphila in adipocytes	14
Expanding the upper‐temperature boundary for the microalga Picochlorum sp. (BPE23) by adaptive laboratory evolution	14
Epitope screening using Hydrogen/Deuterium Exchange Mass Spectrometry (HDX‐MS): An accelerated workflow for evaluation of lead monoclonal antibodies	14
Complete or periodic continuity in continuous manufacturing platforms for production of monoclonal antibodies?	14
Data integrity within the biopharmaceutical sector in the era of Industry 4.0	14
Chitosan and silver nanoparticles are attractive auxin carriers: A comparative study on the adventitious rooting of microcuttings in apple rootstocks	14
Simultaneous production of 2′‐fucosyllactose and difucosyllactose by engineered Escherichia coli with high secretion efficiency	13
Production of Modified Vaccinia Ankara Virus by Intensified Cell Cultures: A Comparison of Platform Technologies for Viral Vector Production	13
CRISPR/Cas9 mediated targeted mutagenesis of LIGULELESS‐1 in sorghum provides a rapidly scorable phenotype by altering leaf inclination angle	13
Selective ene‐reductase immobilization to magnetic nanoparticles through a novel affinity tag	13
Molecular Targets for the Testing of COVID‐19	13
Insight into the bioabsorption of Fe‐based materials and their current developments in bone applications	13
Biotechnological production of the European corn borer sex pheromone in the yeast Yarrowia lipolytica	12
Process economics evaluation of cell‐free synthesis for the commercial manufacture of antibody drug conjugates	12
Multimodal Imaging and Soft X‐Ray Tomography of Fluorescent Nanodiamonds in Cancer Cells	12
Microfluidic fabrication of tunable alginate‐based microfibers for the stable immobilization of enzymes	12
Subcloning induces changes in the DNA‐methylation pattern of outgrowing Chinese hamster ovary cell colonies	12
Detection of four foodborne pathogens based on magnetic separation multiplex PCR and capillary electrophoresis	12
Intravenous injection of mesenchymal stem cell spheroids improves the pulmonary delivery and prolongs in vivo survival	12
Definitive screening accelerates Taxol biosynthetic pathway optimization and scale up in Saccharomyces cerevisiae cell factories	12
Hydrostatic pressure promotes chondrogenic differentiation and microvesicle release from human embryonic and bone marrow stem cells	11
Unraveling the promise and limitations of CRISPR/Cas system in natural product research: Approaches and challenges	11
Humanization of Chicken‐Derived scFv Using Yeast Surface Display and NGS Data Mining	11
Photosynthetic Reduction of Xylose to Xylitol Using Cyanobacteria	11
Simplifying plant gene silencing and genome editing logistics by a one‐Agrobacterium system for simultaneous delivery of multipartite virus vectors	11
Novel anion exchange membrane chromatography method for the separation of empty and full adeno‐associated virus	11
Chemoenzymatic Total Syntheses of Artonin I with an Intermolecular Diels–Alderase	11
Key advances in biocatalytic phosphorylations in the last two decades: Biocatalytic syntheses in vitro and biotransformations in vivo (in humans)	11
Optimization of CRISPR‐Cas9 through promoter replacement and efficient production of L‐homoserine in Corynebacterium glutamicum	11
Assessing the Combinatorial Chemo‐Photothermal Therapy Mediated by Sulfobetaine Methacrylate‐Functionalized Nanoparticles in 2D and 3D In Vitro Cancer Models	11
Optimization of Alcohol Dehydrogenase for Industrial Scale Oxidation of Lactols	11
Accelerating HIV‐1 VLP production using stable High Five insect cell pools	11
Application of microfluidic technology in antibody screening	10
CRISPRa/i with Adaptive Single Guide Assisted Regulation DNA (ASGARD) mediated control of Chlorella sorokiniana to enhance lipid and protein production	10
A combined pH and temperature precipitation step facilitates the purification of tobacco‐derived recombinant proteins that are sensitive to extremes of either parameter	10
Targeting metabolic driving and minimization of by‐products synthesis for high‐yield production of D‐pantothenate in Escherichia coli	10
Comprehensive characterization of dihydrofolate reductase‐mediated gene amplification for the establishment of recombinant human embryonic kidney 293 cells producing monoclonal antibodies	10
Microaerobic growth‐decoupled production of α‐ketoglutarate and succinate from xylose in a one‐pot process using Corynebacterium glutamicum	10
Nitrilase immobilization and transposition from a micro‐scale batch to a continuous process increase the nicotinic acid productivity	10
Donepezil hydrochloride as a novel inducer for osteogenic differentiation of mesenchymal stem cells on PLLA scaffolds in vitro	10
Error‐free recombination in sugarcane mediated by only 30 nucleotides of homology and CRISPR/Cas9 induced DNA breaks or Cre‐recombinase	10
Plasmid Design for Tunable Two‐Enzyme Co‐Expression Promotes Whole‐Cell Production of Cellobiose	10
Overexpression of ER Protein Processing and Apoptosis Regulator Genes in Human Embryonic Kidney 293 Cells Improves Gene Therapy Vectors Production	10
Constructing E. coli Co‐Cultures for De Novo Biosynthesis of Natural Product Acacetin	10
Exosomes: Biogenesis, targeting, characterization and their potential as "Plug & Play" vaccine platforms	10
Benzoate Synthesis from Glucose or Glycerol Using Engineered Pseudomonas taiwanensis	10
Rational engineering and applications of functional bioadhesives in biomedical engineering	10
Cell‐free in vitro reduction of carboxylates to aldehydes: With crude enzyme preparations to a key pharmaceutical building block	10
Systematizing the different notions of growth‐coupled product synthesis and a single framework for computing corresponding strain designs	10
CRISPR/Cas9‐mediated knockout of a prolyl‐4‐hydroxylase subfamily in Nicotiana benthamiana using DsRed2 for plant selection	10
The Effect of Container Surface Passivation on Aggregation of Intravenous Immunoglobulin Induced by Mechanical Shock	10
High cysteine concentrations in cell culture media lead to oxidative stress and reduced bioprocess performance of recombinant CHO cells	10
Recent advances in engineering of microbial cell factories for intelligent pH regulation and tolerance	10
Comparison of mechanistic and hybrid modeling approaches for characterization of a CHO cultivation process: Requirements, pitfalls and solution paths	10
Metabolomic analysis improves bioconversion of methanol to isobutanol in Methylorubrum extorquens AM1	9
Reconstitution of an Anti‐HER2 Antibody Paratope by Grafting Dual CDR‐Derived Peptides onto a Small Protein Scaffold	9
Effect of vitamins and metal ions on productivity and charge heterogeneity of IgG1 expressed in CHO cells	9
Curcumin biosynthesis from ferulic acid by engineered Saccharomyces cerevisiae	9
Analysis of Transformed Upstream Bioprocess Data Provides Insights into Biological System Variation	9
The Degree and Length of O‐Glycosylation of Recombinant Proteins Produced in Pichia pastoris Depends on the Nature of the Protein and the Process Type	9
Advances in production and structural derivatization of the promising molecule ursolic acid	9
Cell‐surface binding domains from Clostridium cellulovorans can be used for surface display of cellulosomal scaffoldins in Lactococcus lactis	9
Non‐invasive and time‐resolved measurement of the respiration activity of Chinese hamster ovary cells enables prediction of key culture parameters in shake flasks	9
An efficient cell‐free protein synthesis platform for producing proteins with pyrrolysine‐based noncanonical amino acids	9
Longitudinal monitoring of cell metabolism in biopharmaceutical production using label‐free fluorescence lifetime imaging microscopy	9
Pan‐Genome‐Scale Network Reconstruction: Harnessing Phylogenomics Increases the Quantity and Quality of Metabolic Models	9
CRISPR with a Happy Ending: Non‐Templated DNA Repair for Prokaryotic Genome Engineering	9
Process optimization, metabolic engineering interventions and commercialization of microbial polyhydroxyalkanoates production – A state‐of‐the art review	9
Epitopes of Naturally Acquired and Vaccine‐Induced Anti‐Ebola Virus Glycoprotein Antibodies in Single Amino Acid Resolution	9
Multiomics approaches and genetic engineering of metabolism for improved biorefinery and wastewater treatment in microalgae	9
Engineering a thermostable chondroitinase for production of specifically distributed low‐molecular‐weight chondroitin sulfate	8
Key criteria for engineering mycotoxin binding aptamers via computational simulations: Aflatoxin B1 as a case study	8
Designed SARS‐CoV‐2 receptor binding domain variants form stable monomers	8
Continuous optical in‐line glucose monitoring and control in CHO cultures contributes to enhanced metabolic efficiency while maintaining darbepoetin alfa product quality	8
A Site‐Specific Integration Reporter System That Enables Rapid Evaluation of CRISPR/Cas9‐Mediated Genome Editing Strategies in CHO Cells	8
Genome Editing‐accelerated Re‐Domestication (GEaReD) – A new major direction in plant breeding	8
Single‐strand annealing: Molecular mechanisms and potential applications in CRISPR‐Cas‐based precision genome editing	8
Umbilical cord derived mesenchymal stromal cells in microcarrier based industrial scale culture sustain the immune regulatory functions	8

Carotenoid fortification of zucchini fruits using a viral RNA vector	8
Methods for addressing host cell protein impurities in biopharmaceutical product development	8
PASylation Enhances the Stability, Potency, and Plasma Half‐Life of Interferon α‐2a: A Molecular Dynamics Simulation	8
Study and Evaluation of the Potential of Lipid Nanocarriers for Transdermal Delivery of siRNA	8
Physiochemical properties of enveloped viruses and arginine dictate inactivation	8
Transcription Factor ArcA is a Flux Sensor for the Oxygen Consumption Rate inEscherichia coli	8
Assessing the potential of Schizochytrium sp. HX‐308 for microbial lipids production from corn stover hydrolysate	8
Advances in Bacterial Biofilm Management for Maintaining Microbiome Homeostasis	8
Modeling the Effect of Amino Acids and Copper on Monoclonal Antibody Productivity and Glycosylation: A Modular Approach	8
Crystal Contact Engineering Enables Efficient Capture and Purification of an Oxidoreductase by Technical Crystallization	8
IPTG‐induced high protein expression for whole‐cell biosynthesis of L‐phosphinothricin	7
Heterologous expression of cyanobacterial gas vesicle proteins in Saccharomyces cerevisiae	7
Prevention of nosocomial transmission and biofilm formation on novel biocompatible antimicrobial gloves impregnated with biosynthesized silver nanoparticles synthesized using Eucalyptus citriodora<	7
Rsn‐2‐mediated directed foam enrichment of β‐lactamase	7
Near‐infrared light responsive dendrimers facilitate the extraction of bicalutamide from human plasma and urine	7
Parallel and large‐scale antitumor investigation using stable chemical gradient and heterotypic three‐dimensional tumor coculture in a multi‐layered microfluidic device	7
Inhibition of E. coli Host RNA Polymerase Allows Efficient Extracellular Recombinant Protein Production by Enhancing Outer Membrane Leakiness	7
Fluorescence‐based screening for engineered aldo‐keto reductase KmAKR with improved catalytic performance and extended substrate scope	7
A highly sensitive PNA‐microarray system for miRNA122 recognition	7
Converting a mAb downstream process from batch to continuous using process modeling and process analytical technology	7
Vaccine and vaccination as a part of human life: In view of COVID‐19	7
Lyophilization of premixed COVID‐19 diagnostic RT‐qPCR reactions enables stable long‐term storage at elevated temperature	7
Cross‐neutralization of RBD mutant strains of SARS‐CoV‐2 by convalescent patient derived antibodies	7
Increased recombinant adeno‐associated virus production by HEK293 cells using small molecule chemical additives	7
Medium optimization and subsequent fermentative regulation enabled the scaled‐up production of anti‐tuberculosis drug leads ilamycin‐E1/E2	7
Modulating tyrosine sulfation of recombinant antibodies in CHO cell culture by host selection and sodium chlorate supplementation	7
Integrated process development: The key to improve Fab production in E. coli	7
Rational hinge engineering of carboxylic acid reductase from Mycobacterium smegmatis enhances its catalytic efficiency in biocatalysis	7
An engineered microfluidic blood‐brain barrier model to evaluate the anti‐metastatic activity of β‐boswellic acid	7
Advancing Raman model calibration for perfusion bioprocesses using spiked harvest libraries	7
Spinal cord regeneration: A brief overview of the present scenario and a sneak peek into the future	7
Gas‐Sensing Transcriptional Regulators	7
Biofabrication of a three dimensional human‐based personalized neurofibroma model	7
Mechanistic discoveries and simulation‐guided assay optimization of portable hormone biosensors with cell‐free protein synthesis	7
Degenerative disease‐on‐a‐chip: Developing microfluidic models for rapid availability of newer therapies	7
Recombineering facilitates the discovery of natural product biosynthetic pathways in Pseudomonas parafulva	7
Auxotrophs compromise cell growth and fatty acid production in Saccharomyces cerevisiae	7
Promoting cell growth and characterizing partial symbiotic relationships in the co‐cultivation of green alga Chlamydomonas reinhardtii and Escherichia coli	7
A BioBricks toolbox for metabolic engineering of the tetracenomycin pathway	7
Forskolin Increases cAMP Levels and Enhances Recombinant Antibody Production in CHO Cell Cultures	7
The SSV‐Seq 2.0 PCR‐Free Method Improves the Sequencing of Adeno‐Associated Viral Vector Genomes Containing GC‐Rich Regions and Homopolymers	7
Glycosidic Linkage Structures Influence Dietary Fiber Fermentability and Propionate Production by Human Colonic Microbiota In Vitro	7
Determinants of the Nucleotide Specificity in the Carbohydrate Epimerase Family 1	7
An integrated modular framework for modeling the effect of ammonium on the sialylation process of monoclonal antibodies produced by CHO cells	7
Experimental aspects of NPY‐decorated gold nanoclusters using randomized hybrid design against breast cancer cell line	7
Microscale Perfusion‐Based Cultivation for Pichia pastoris Clone Screening Enables Accelerated and Optimized Recombinant Protein Production Processes	7
Reactor control system in bacterial co‐culture based on fluorescent proteins using an Arduino‐based home‐made device	6
Discovery and Characterization of a Novel Thermostable β‐Amino Acid Transaminase from a Meiothermus Strain Isolated in an Icelandic Hot Spring	6
Amorphous Polyphosphate and Ca‐Carbonate Nanoparticles Improve the Self‐Healing Properties of both Technical and Medical Cements	6
Coupling picodroplet microfluidics with plate imaging for the rapid creation of biomanufacturing suitable cell lines with high probability and improved multi‐step assurance of monoclonality	6
Model‐driven design of synthetic N‐terminal coding sequences for regulating gene expression in yeast and bacteria	6
Rapid regulations of metabolic reactions in Escherichia coli via light‐responsive enzyme redistribution	6
Metabolic regulation and optimization of oxygen supply enhance the 2,3‐butanediol yield of the novel Klebsiella sp. isolate FSoil 024	6
Establishment of a pig CRISPR/Cas9 knockout library for functional gene screening in pig cells	6
Oligonucleotide abundance biases aid design of a type IIS synthetic genomics framework with plant virome capacity	6
Using lysozyme amyloid fibrils as a means of scavenging aggregation‐inhibiting compounds	6
Biocatalytic synthesis of 2‐O‐α‐D‐glucopyranosyl‐L‐ascorbic acid using an extracellular expressed α‐glucosidase from Oryza sativa	6
Engineering of the CMV promoter for controlled expression of recombinant genes in HEK293 cells	6
The role of the CRISPR‐Cas system in cancer drug development: Mechanisms of action and therapy	6
Deletion analysis of Pichia pastoris alcohol dehydrogenase 2 (ADH2) promoter and development of synthetic promoters	6
High fidelity one‐pot DNA assembly using orthogonal serine integrases	6
Improvement of acetyl‐CoA supply and glucose utilization increases l‐leucine production in Corynebacterium glutamicum	6
Capillary and microchip electrophoresis method development for amino acid monitoring during biopharmaceutical cultivation	6
Computational fluid dynamics simulation improves the design and characterization of a plug‐flow‐type scale‐down reactor for microbial cultivation processes	6
Quercetin‐loaded platelets as a potential targeted therapy for glioblastoma multiforme cell line U373‐MG	6
Building a machine‐learning model to predict optimal mevalonate pathway gene expression levels for efficient production of a carotenoid in yeast	6
l‐Lactate oxidase‐mediated removal of l‐lactic acid derived from fermentation medium for the production of optically pure D‐lactic acid	6
Optimization of a transient antibody expression platform towards high titer and efficiency	6
Homologous Recombination Offers Advantages over Transposition‐Based Systems to Generate Recombinant Baculovirus for Adeno‐Associated Viral Vector Production	6
Chemical modification of lipases: A powerful tool for activity improvement	6
Anti‐Parkinson's Disease Function of Dioscin‐Zein‐Carboxymethyl Cellulose Nanocomplex in Caenorhabditis elegans	6
Direct capture and selective elution of a secreted polyglutamate‐tagged nanobody using bare magnetic nanoparticles	6
Improving Antibody Production in Stably Transfected CHO Cells by CRISPR‐Cas9‐Mediated Inactivation of Genes Identified in a Large‐Scale Screen with Chinese Hamster‐Specific siRNAs	6
Stable overexpression of mutated PTEN in Chinese hamster ovary cells enhances their performance and therapeutic antibody production	6
Knockout of Sf‐Caspase‐1 generates apoptosis‐resistant Sf9 cell lines: Implications for baculovirus expression	6
Recent Progress of Hybrid Optical Probes for Neural Membrane Potential Imaging	6
CpPosNeg: A positive‐negative selection strategy allowing multiple cycles of marker‐free engineering of the Chlamydomonas plastome	6
Supplementation of critical amino acids improves glycerol and lactose uptake and enhances recombinant protein production in Escherichia coli	6
Novel erythropoietin‐based therapeutic candidates with extra N‐glycan sites that block hematopoiesis but preserve neuroplasticity	6
Designing microfluidic devices for behavioral screening of multiple zebrafish larvae	6
Boosting production of cembratriene‐ol in Saccharomyces cerevisiae via systematic optimization	6
A seasonal simulation approach for culture depth influence on the temperature for different characterized microalgae strains	6
Magnetic resonance imaging as a tool for quality control in extrusion‐based bioprinting	6
Directed Reaction Engineering Boosts Succinate Formation of Synechocystis sp. PCC 6803_Δsll1625	6
Virus inactivation at moderately low pH varies with virus and buffer properties	6
A tailored LNA clamping design principle: Efficient, economized, specific and ultrasensitive for the detection of point mutations	6