Biotechnology Journal

Article	Citations
	120
Osteochondral Tissue Engineering: Scaffold Materials, Fabrication Techniques and Applications	93
Engineering a 3‐dimensional tissue construct with adipose‐derived stem cells for healing bone defect: An ex vivo study with femur head	79
Live‐cell imaging of genomic loci with Cas9 variants	41
Overexpression of Geranylgeranyl Diphosphate Synthase and Cyclase Enhances Pleuromutilin Production in Clitopilus Passeckerianus T6	38
Multifunctional PAMAM Dendrimers Carrying SAHA, 5‐FU, and a Therapeutic Gene for Targeted Co‐Delivery Toward Colorectal Cancer Cells	38
Advancing a rapid, high throughput screening platform for optimization of lentivirus production	30
Site specific insertion of a transgene into the murine α‐casein (CSN1S1) gene results in the predictable expression of a recombinant protein in milk	30
Cell fate‐inducing CARs orthogonally control multiple signaling pathways	30
Expression and application of aflatoxin degrading enzyme gene in Pichia pastoris	29
Feeding strategies for Sporosarcina pasteurii cultivation unlock more efficient production of ureolytic biomass for MICP	28
Intermediate product control in cascade reaction for one‐pot production of ε‐caprolactone by Escherichia coli	28
Design and evaluation of a 3D‐printed, lab‐scale perfusion bioreactor for novel biotechnological applications	28
A generic pump‐free organ‐on‐a‐chip platform for assessment of intestinal drug absorption	28
Improved synthesis of dietary triglycerides by using lipase supported on clay carriers	27
Dialysis rolled scaffold bioreactor allows extended production of monoclonal antibody with reduced media use	27
Biomedical application of microalgal‐biomaterials hybrid system	27
Engineering of the start condensation domain with improved N‐decanoyl catalytic activity for daptomycin biosynthesis	25
Multiomics approaches and genetic engineering of metabolism for improved biorefinery and wastewater treatment in microalgae	25
Scaling strategy for cell and gene therapy bioreactors based on turbulent parameters	24
Numerical Simulation and Comparison of Flow Field in Different Dynamic Co‐Culture Conditions	24
Potential of butanol production from Thailand marine macroalgae using Clostridium beijerinckii ATCC 10132‐based ABE fermentation	24
Through virtual saturation mutagenesis and rational design for superior substrate conversion in engineered d‐amino acid oxidase	24
CRISPR/Cas9 mediated targeted mutagenesis of LIGULELESS‐1 in sorghum provides a rapidly scorable phenotype by altering leaf inclination angle	23
Toehold switch plus signal amplification enables rapid detection	23

Redesigning glycolaldehyde synthase for the synthesis of biorefinery feedstock D‐xylulose from C1 compounds	22
Bio‐based polymers containing traditional medicinal fillers for wound healing applications – An evaluation of neoteric development and future perspectives	21
Outside Front Cover: (Biotechnology Journal 10/2022)	21
Effect of High‐Speed Shaking on Oxygen Transfer in Shake Flasks	21
Editorial Board: Biotechnology Journal 12/2021	21
A Competition‐Based Strategy for the Isolation of an Anti‐Idiotypic Blocking Module and Fine‐Tuning for Conditional Activation of a Therapeutic Antibody	21
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Optimization of Isoamylase Production in Escherichia coli Under Different Nutrient Limitation Conditions	20
Rapid At‐Line AAVX Affinity HPLC: Enabling Process Analytical Technology for Bioprocess Development of Adeno‐Associated Virus Vectors	20
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Massively parallel DNA target capture using long adapter single stranded oligonucleotide (LASSO) probes assembled through a novel DNA recombinase mediated methodology	19
Microalgae‐made human vaccines and therapeutics: A decade of advances	19
Comparison of protein quantification methods for protein encapsulation with ZIF‐8 metal‐organic frameworks	19
Synthetic Osmolytes for Enhanced RNA Thermotolerance and Extended Plasmid Storage at Room Temperature	19
A high‐fidelity, dual site‐specific integration system in CHO cells by a Bxb1 recombinase	19
A new look at the fluorescent protein‐based approach for identifying optimal coding sequence for recombinant protein expression in E. coli	18
Systematizing the different notions of growth‐coupled product synthesis and a single framework for computing corresponding strain designs	18
The evaluation of biotechnological potential of Gp144, the key molecule of natural predator bacteriophage K in Staphylococcus aureus hunting mechanism	17
Enhancing production and purity of 9‐OH‐AD from phytosterols by balancing metabolic flux of the side‐chain degradation and 9‐position hydroxylation in Mycobacterium neoaurum	17
In vitro acne disease model from inertial focusing effect for studying the interactions between sebocyte glands and macrophages	17
Characterization and discrimination of spike protein in SARS‐CoV‐2 virus‐like particles via surface‐enhanced Raman spectroscopy	17
Metabolic engineering of Aspergillus niger for accelerated malic acid biosynthesis by improving NADPH availability	16
Synergisms of machine learning and constraint‐based modeling of metabolism for analysis and optimization of fermentation parameters	16
Donepezil hydrochloride as a novel inducer for osteogenic differentiation of mesenchymal stem cells on PLLA scaffolds in vitro	16
Combination of MAR and intron increase transgene expression of episomal vectors in CHO cells	16
Fabrication of a decellularized liver matrix–based hepatic patch for the repair of CCl4‐induced liver injury	16
Multiomics analysis reveals the biological effects of live Roseburia intestinalis as a high‐butyrate‐producing bacterium in human intestinal epithelial cells	16
Experimental aspects of NPY‐decorated gold nanoclusters using randomized hybrid design against breast cancer cell line	16
Direct capture and selective elution of a secreted polyglutamate‐tagged nanobody using bare magnetic nanoparticles	16
Yield improvement of enediyne yangpumicins in Micromonospora yangpuensis through ribosome engineering and fermentation optimization	16
Surrogate model to screen for inactivation‐based clearance of enveloped viruses during biotherapeutics process development	16
Single‐particle fluorescence tracking combined with TrackMate assay reveals highly heterogeneous and discontinuous lysosomal transport in freely orientated axons	15
Single‐cell genotyping of phytoplankton from ocean water by gel‐based cell manipulation	15
DNA origami scaffold promoting nerve guidance and regeneration	15
Editorial Board: Biotechnology Journal 3/2022	15
Mimicking continuous capture chromatography for virus clearance using a single chromatography column model	15
Biomass specific perfusion rate as a control lever for the continuous manufacturing of biosimilar monoclonal antibodies from CHO cell cultures	15
Investigation of the Effects of Applying Bio‐Magnesium Oxide Nanoparticle Fertilizer to Moringa Oleifera Plants on the Chemical and Vegetative Properties of the Plants’ leaves	15
A single‐cell genomic strategy for alternative transcript start sites identification	15
Dopaminergic signaling regulates zebrafish larvae's response to electricity	15
Cas9‐orthologue‐mediated cytosine and adenine base editors recognizing NNAAAA PAM sequences	15
Non‐invasive and time‐resolved measurement of the respiration activity of Chinese hamster ovary cells enables prediction of key culture parameters in shake flasks	15
Highly efficient identification of nucleocytoplasmic O‐glycosylation by the TurboID‐based proximity labeling method in living cells	14
Improving bioluminescence of a minimal luciferase by adding a charged oligopeptide: picALuc2.0	14
Oriented in situ immobilization of a functional tyrosinase on microcrystalline cellulose effectively incorporates DOPA residues in bioengineered mussel adhesive protein	14
Enhanced synthesis of S‐adenosyl‐L‐methionine through combinatorial metabolic engineering and Bayesian optimization in Saccharomyces cerevisiae	14
Outside Front Cover: (Biotechnology Journal 6/2021)	14
Promoter screening and identification for metabolic regulation in Acremonium chrysogenum	14
Magnetic resonance imaging as a tool for quality control in extrusion‐based bioprinting	14
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Outside Front Cover: (Biotechnology Journal 5/2023)	13
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Editorial Board: Biotechnology Journal 1/2023	13
Preparation of Thermosensitive Lignocellulose Hollow Fiber Membrane Grafted With PNIPAAm and Its Application as a Cell Culture Carrier in a RSOC Dynamic Culture	13
Outside Front Cover: (Biotechnology Journal 6/2023)	13
Issue Information: Biotechnology Journal 12/2024	13
Dissecting cell death pathways in fed‐batch bioreactors	12
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Editorial Board: Biotechnology Journal 1/2024	12
Exosomal lncRNA USP30‐AS1 activates the Wnt/β‐catenin signaling pathway to promote cervical cancer progression via stabilization of β‐catenin by USP30	12
Lipofection with Lipofectamine™ 2000 in a heparin‐free growth medium results in high transfection efficiency in chicken primordial germ cells	11
Lipidomic and transcriptomic analysis of the increase in eicosapentaenoic acid under cobalamin deficiency of Schizochytrium sp	11
Expanding the targeting scope of FokI‐dCas nuclease systems with SpRY and Mb2Cas12a	11
Enhancing the expression of terminal deoxynucleotidyl transferases by N‐terminal truncation	11
Successful reversal of transgene silencing in Chlamydomonas reinhardtii	11
Proteomic workflows for deep phenotypic profiling of 3D organotypic liver models	11
Enhanced targeting: Production of filamentous bacteriophage monoclonal antibodies using the major coat protein pVIII as anchor	11
Dinucleotide tag‐based parallel reporter gene assay method enables efficient identification of regulatory mutations	11
A machine learning‐based approach for improving plasmid DNA production in Escherichia coli fed‐batch fermentations	11
Improved adeno‐associated virus empty and full capsid separation using weak partitioning multi‐column AEX chromatography	11
Development of a biotechnology process for the production of a novel hyperglycosylated long‐acting recombinant bovine follicle‐stimulating hormone	11
Metabolic engineering of threonine catabolism enables Saccharomyces cerevisiae to produce propionate under aerobic conditions	11
Continuous phase separation of stable emulsions from biphasic whole‐cell biocatalysis by catastrophic phase inversion	11
Nitrilase immobilization and transposition from a micro‐scale batch to a continuous process increase the nicotinic acid productivity	11
YBac: A novel and simple baculovirus system for heterologous protein production in Spodoptera frugiperda 9 (Sf9) cells	11
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Chromatin Accessibility Plays an Important Epigenetic Role on Antibody Expression From CMV Promoter and DNA Elements Flanking the CHO TI Host Landing‐Pad	10
Autofluorescence prediction model for fluorescence unmixing and age determination	10
The economic value of genetically engineered mosquitoes as a malaria control strategy depends on local transmission rates	10
Simultaneous production of 2′‐fucosyllactose and difucosyllactose by engineered Escherichia coli with high secretion efficiency	10
Efficient production of 22(R)‐hydroxycholesterol via combination optimization of Saccharomyces cerevisiae	10
Editorial Board: Biotechnology Journal 10/2021	10
Exploring nutrient supplementation and bioprocess optimization to improve the production of lentiviral vectors in serum‐free medium suspension cultures	10
Development and optimization of Lysis gene E as a counter‐selection marker with high stringency	10
Benchmarking and optimization of a high‐throughput sequencing based method for transgene sequence variant analysis in biotherapeutic cell line development	10
Liamocins overproduction via the two‐pH stage fermentation and anti‐Aspergillus flavus activity of Massoia lactone	10
A 3D paper microfluidic device for enzyme‐linked assays: Application to DNA analysis	10
Editorial Board: Biotechnology Journal 6/2022	10
Recombinant human fibroblast growth factor 7 obtained from stable Chinese hamster ovary cells enhances wound healing	10
Toward standardized solid medium cultivations: Online microbial monitoring based on respiration activity	10
Development, optimization, and scale‐up of suspension Vero cell culture process for high titer production of oncolytic herpes simplex virus‐1	10
mRNA Vaccines: Design Principles, Mechanisms, and Manufacturing—Insights From COVID‐19 as a Model for Combating Infectious Diseases	10
Hierarchical time‐series analysis of dynamic bioprocess systems	10
Semi‐rational engineering of D‐allulose 3‐epimerase for simultaneously improving the catalytic activity and thermostability based on D‐allulose biosensor	10
Integrating Biofilm‐Based Fermentation With Continuous Processes for Improved L‐Valine Production	9
Chicken‐Derived Single‐Chain Variable Fragments Targeting Monkeypox Virus A29L Protein	9
Recent advances and fundamentals of microalgae cultivation technology	9
Design of a microfluidic mixer channel: First steps into creating a fluorescent dye‐based biosensor for mAb aggregate detection	9
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Gulo gene locus, a new gene editing locus for mammalian cells	9
3D‐QSAR pharmacophore modeling, virtual screening, molecular docking, MD simulations, in vitro and in vivo studies to identify potential anti‐hyperplasia drugs	9
Comprehensive mRNA‐sequencing‐based characterization of three HEK‐293 cell lines during an rAAV production process for gene therapy applications	9
Proteomic analysis reveals that the co‐ordination of cytosolic and mitochondrial pathways is beneficial for sabinene biosynthesis in engineered Saccharomyces cerevisiae	9
Generation of reproductive transgenic pigs of a CRISPR‐Cas9‐based oncogene‐inducible system by somatic cell nuclear transfer	9
Octadecyl and sulfonyl modification of diatomite synergistically improved the immobilization efficiency of lipase and its application in the synthesis of pine sterol esters	9
Electrochemical Glucose Sensors: Classification, Catalyst Innovation, and Sampling Mode Evolution	9
Editorial Board: Biotechnology Journal 8/2021	9
Clioquinol rescues yeast cells from Aβ42 toxicity via the inhibition of oxidative damage	9
Secretory production of 7‐dehydrocholesterol by engineered Saccharomyces cerevisiae	9
The Mechanism Underlying Enhanced Coal‐to‐Methane Conversion in Anaerobic Digestion With Betaine Supplementation	9
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Construction of a Cell Factory for the Targeted and Efficient Production of Phytosterol to Boldenone in Mycobacterium neoaurum	9
Bioinspired 3D microprinted cell scaffolds: Integration of graph theory to recapitulate complex network wiring in lymph nodes	9
Editorial Board: Biotechnology Journal 4/2022	9
Transcriptional Downregulation of Methanol Metabolism Key Genes During Yeast Death in Engineered Pichia pastoris	9
Development of an efficient liposomal DOX delivery formulation for HCC therapy by targeting CK2α	9
Stable overexpression of mutated PTEN in Chinese hamster ovary cells enhances their performance and therapeutic antibody production	9
Liamocin biosynthesis is induced by an autogenous host acid activation in Aureobasidium melanogenum	8
Amino acid degradation pathway inhibitory by‐products trigger apoptosis in CHO cells	8
Impact of Eco‐Friendly Surfactant Structure and Class on Enveloped Virus Inactivation	8
1H NMR‐based process understanding and biochemical marker identification methodology for monitoring CHO cell culture process during commercial‐scale manufacturing	8
Characterization of the Ubiquitin‐Modified Proteome of Recombinant Chinese Hamster Ovary Cells in Response to Endoplasmic Reticulum Stress	8
Resolving the Trade‐Off Between Toxicity and Efficiency of CRISPR‐Cas9 System for Genome Editing Within Escherichia coli	8
Dual‐layered hydrogels allow complete genome recovery with nucleic acid cytometry	8
Efficient production of glutathione in Saccharomyces cerevisiae via a synthetic isozyme system	8
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Model‐driven design of synthetic N‐terminal coding sequences for regulating gene expression in yeast and bacteria	8
Antibacterial functionalization of calfskin bioremediation film	8
Molecular design of controllable recombinant adeno‐associated virus (AAV) expression systems for enhanced vector production	8
A 3D bioprinted antibacterial hydrogel dressing of gelatin/sodium alginate loaded with ciprofloxacin hydrochloride	8

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Expanding the toolbox of probiotic Escherichia coli Nissle 1917 for synthetic biology	8
L‐malic acid production from xylose by engineered Saccharomyces cerevisiae	8
The Experimental Study of Double‐Layer Heterogeneous CA Scaffold in Promoting the Surface Shape Recovery and Internal Osteogenesis of Alveolar Bone	8
Rational Design of a Novel DNA Polymerase From Clostridium thermocellum to Improve LAMP Detection Performance	8
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Evaluation of expression systems for recombinant protein production in chicken egg bioreactors	8
A single‐cell surgery microfluidic device for transplanting tumor cytoplasm into dendritic cells without nuclei mixing	8
Identification of critical process parameters for expansion of clinical grade human Wharton's jelly‐derived mesenchymal stromal cells in stirred‐tank bioreactors	8
In vitro evolution of diagnostic antibodies targeting native antigens in plasma by sandwich flow cytometry	8
Initial cyclic‐di‐GMP upregulation triggers sporadic cellular expansion leading to improved cellular survival	8
Metabolic regulation and optimization of oxygen supply enhance the 2,3‐butanediol yield of the novel Klebsiella sp. isolate FSoil 024	8
Single‐strand annealing: Molecular mechanisms and potential applications in CRISPR‐Cas‐based precision genome editing	8
Concentration‐Dependent Effects of MXene Nanocomposite‐Loaded Carboxymethyl Cellulose on Wound Healing	8
Outside Front Cover: (Biotechnology Journal 10/2023)	8
Promoting cell growth and characterizing partial symbiotic relationships in the co‐cultivation of green alga Chlamydomonas reinhardtii and Escherichia coli	8
In vitro and in silico analyses reveal the interaction between LysM receptor‐like kinase3 of Solanum tuberosum and the carbohydrate elicitor Riclin octaose	8
Factors and methods to modulate DNA hybridization kinetics	7
Ultrasound assisted in situ separation of sophorolipids in multi‐phase fermentation system to achieve efficient production by Candida bombicola	7
Light‐induced fermenter production of derivatives of the sweet protein monellin is maximized in prestationary Saccharomyces cerevisiae cultures	7
Expanding the upper‐temperature boundary for the microalga Picochlorum sp. (BPE23) by adaptive laboratory evolution	7
Drug‐loaded lipid nanoparticles improve the removal rates of the Staphylococcus aureus biofilm	7
Study and Application of Microbial Community in Biodegradation of Kitchen Waste Using High‐Throughput Sequencing and Strain Isolation Techniques	7
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Biosynthesis of the antioxidant γ‐glutamyl‐cysteine with engineered Yarrowia lipolytica	7
A biodegradable, mechanically tunable micro‐arc oxidation AZ91D‐based composite implant with calcium phosphate/chitosan coating promotes long‐term bone tissue regeneration	7
Phage display selection of a Pb(II) specific peptide and its application as a biorecognition unit for colorimetric detection of Pb(II) ions	7
Cross‐neutralization of RBD mutant strains of SARS‐CoV‐2 by convalescent patient derived antibodies	7
Use of tRNA gene barriers improves stability of transgene expression in CHO cells	7
Towards a Platform Chromatography Purification Process for Adeno‐Associated Virus (AAV)	7
Arg128*‐Mediated Dual‐Substrate Recognition and Dynamic Transport Mechanisms in (R)‐ω‐Transaminase: Computational Insights and Mutational Profiling Guided Rational Engineering	7
Computational evaluation of light propagation in cylindrical bioreactors for optogenetic mammalian cell cultures	7
Development of a novel microplate for high‐throughput screening and optimization of DHA producing strains based on CFD technology	7
CRISPRa/i with Adaptive Single Guide Assisted Regulation DNA (ASGARD) mediated control of Chlorella sorokiniana to enhance lipid and protein production	7
Rapid construction of Escherichia coli chassis with genome multi‐position integration of isopentenol utilization pathway for efficient and stable terpenoid accumulation	7
An improved lentiviral system for efficient expression and purification of β‐defensins in mammalian cells	7
Outside Front Cover: (Biotechnology Journal 12/2022)	7
Recombineering facilitates the discovery of natural product biosynthetic pathways in Pseudomonas parafulva	7
Knowledge extraction from literature and enzyme sequences complements FBA analysis in metabolic engineering	7
Aptabinding of tumor necrosis factor‐α (TNFα) inhibits its proinflammatory effects and alleviates islet inflammation	7
Deletion analysis of Pichia pastoris alcohol dehydrogenase 2 (ADH2) promoter and development of synthetic promoters	7
Advancements in Plant Gene Editing Technology: From Construct Design to Enhanced Transformation Efficiency	7
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Advances in biosynthesis of peptide drugs: Technology and industrialization	7
In situ formation of alginic acid‐gold nanohybrids for application in cancer photothermal therapy	7
The immune system of prokaryotes: potential applications and implications for gene editing	7
Implementation of RAGATH RNA‐associated DNA Endonucleases as Genome Editing Tool in Escherichia coli	7
Uncertainty quantification for gene delivery methods: A roadmap for pDNA manufacturing from phase I clinical trials to commercialization	7
Editorial Board: Biotechnology Journal 9/2021	7
Advances in promoter engineering: Novel applications and predefined transcriptional control	7
Vaccine and vaccination as a part of human life: In view of COVID‐19	7
Microfabricated dynamic brain organoid cocultures to assess the effects of surface geometry on assembloid formation	7
CRISPR‐Cas enzymes: The toolkit revolutionizing diagnostics	7
Tuning mobile phase properties to improve empty and full particle separation in adeno‐associated virus productions by anion exchange chromatography	7
Issue Information: Biotechnology Journal 7/2024	7
Designed SARS‐CoV‐2 receptor binding domain variants form stable monomers	6
High cysteine concentrations in cell culture media lead to oxidative stress and reduced bioprocess performance of recombinant CHO cells	6
Recognizing CRISPR as the new age disease‐modifying drug: Strategies to bioengineer CRISPR/Cas for direct in vivo delivery	6
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Engineering a versatile yeast platform for sesquiterpene production from glucose or methanol	6
Pairing of orthogonal chaperones with a cytochrome P450 enhances terpene synthesis in Saccharomyces cerevisiae	6
Suspension culture promoted the expansion of NK‐92 cells ex vivo by enhancing the expression of IL‐2 receptor	6
Lung‐specific exosomes for doxorubicin delivery in lung adenocarcinoma therapy	6
Engineering phenylalanine ammonia lyase to limit feedback inhibition by cinnamate and enhance biotransformation	6
Impact of ethanol on continuous inline diafiltration of liposomal drug products	6
Self‐Produced Brain‐Like ECM From 3D‐Cultured Dermal Fibroblasts Enhances Neuronal Growth and Survival	6
A comparative evaluation of layer‐by‐layer assembly techniques for surface modification of microcarriers used in human mesenchymal stromal cell manufacturing	6
Tuning CpG motif position in nanostructured DNA for efficient immune stimulation	6
Cell‐surface binding domains from Clostridium cellulovorans can be used for surface display of cellulosomal scaffoldins in Lactococcus lactis	6
Optimization of CRISPR‐Cas9 through promoter replacement and efficient production of L‐homoserine in Corynebacterium glutamicum	6
Editorial Board: Biotechnology Journal 6/2021	6
Recombinant production of a highly efficient photolyase from Thermus thermophilus	6
Medical Ultrasound Application Beyond Diagnosis: Insights From Ultrasound Sensing and Biological Response	6
Recent advances in the synthesis of single‐stranded DNA in vitro	6
Structure‐based discovery of small molecules improving stability of human broadly‐neutralizing anti‐HIV antibody 2F5 in plant suspension cells	6
Characterisation of the E. coli HMS174 and BLR host cell proteome to guide purification process development	6
Microwell‐based flow culture increases viability and restores drug response in prostate cancer spheroids	6
Methods for addressing host cell protein impurities in biopharmaceutical product development	6
Bacteriophage carriers localize in the brain of a rat model of neonatal hypoxic‐ischemic encephalopathy	6
Combination of direct boiling and glass beads increases the purity and accuracy of bacterial DNA extraction	6
Single in‐line biomass probe detects CHO cell growth by capacitance and bacterial contamination by conductivity in bioreactor	6
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Quantitative human physiology: An introduction guide for advanced tissue engineering	6
Unlocking the formate utilization of wild‐type Yarrowia lipolytica through adaptive laboratory evolution	6
Mitochondrial Oxidative Stress Related Diagnostic Model Accurately Assesses Rheumatoid Arthritis Risk Stratification and Immune Infiltration Characterization	6
The effects of length and sequence of gRNA on Cas13b and Cas13d activity in vitro and in vivo	6
Building a machine‐learning model to predict optimal mevalonate pathway gene expression levels for efficient production of a carotenoid in yeast	6
Continuous optical in‐line glucose monitoring and control in CHO cultures contributes to enhanced metabolic efficiency while maintaining darbepoetin alfa product quality	6
Optimization of muscle cell culture media using nonlinear design of experiments	6
Enhancing the organic solvent resistance of ω‐amine transaminase for enantioselective synthesis of (R)‐(+)‐1(1‐naphthyl)‐ethylamine	6