Biotechnology Journal

Article	Citations
	116
Peptonics: A new family of cell‐protecting surfactants for the recombinant expression of therapeutic proteins in mammalian cell cultures	91
Enhanced anti‐tumor activity of arginine decarboxylase through the incorporation of aromatic amino acids at the multimer‐forming interface	90
	76
A biotechnological approach for the production of new protein bioplastics	38
Antibacterial functionalization of calfskin bioremediation film	38
Development and optimization of a frequency mixing sensor for adjacent samples quantitative detection on a lateral flow assay	37
In vitro and in silico analyses reveal the interaction between LysM receptor‐like kinase3 of Solanum tuberosum and the carbohydrate elicitor Riclin octaose	29
Site specific insertion of a transgene into the murine α‐casein (CSN1S1) gene results in the predictable expression of a recombinant protein in milk	29
Antibody mimetics: The next generation antibody engineering, a retrospective and prospective analysis	29
Customizing amino acid metabolism of Pichia pastoris for recombinant protein production	27
Antimicrobial and antiviral activities with molecular docking study of chitosan/carrageenan@clove oil beads	27
Construction and application of a new CRISPR/Cas12a system in Stenotrophomonas AGS‐1 from aerobic granular sludge	26
Detection of four foodborne pathogens based on magnetic separation multiplex PCR and capillary electrophoresis	26
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Editorial Board: Biotechnology Journal 10/2022	24
Editorial Board: Biotechnology Journal 11/2022	23
Outside Front Cover: (Biotechnology Journal 9/2022)	22
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Optimising protein detection with fixable custom oligo‐labelled antibodies for single‐cell multi‐omics approaches	21
Rsn‐2‐mediated directed foam enrichment of β‐lactamase	21
Outside Front Cover: (Biotechnology Journal 7/2023)	21
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Outside Front Cover: (Biotechnology Journal 12/2021)	20

Subtractive Inhibition Assay Based on PagN‐Specific Monoclonal Antibody for the Detection of Salmonella Using Surface Plasmon Resonance	20
Osteochondral Tissue Engineering: Scaffold Materials, Fabrication Techniques and Applications	20
A 3′‐end capture sequencing method for high‐throughput targeted gene expression profiling	20
Outside Front Cover: (Biotechnology Journal 5/2021)	19
A single‐cell surgery microfluidic device for transplanting tumor cytoplasm into dendritic cells without nuclei mixing	19
Editorial Board: Biotechnology Journal 8/2021	18
Editorial Board: Biotechnology Journal 7/2021	18
Outside Front Cover: (Biotechnology Journal 6/2022)	18
Cell fate‐inducing CARs orthogonally control multiple signaling pathways	18
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Characterization of essential elements for improved episomal expressions in Kluyveromyces marxianus	17
Metabolic regulation and optimization of oxygen supply enhance the 2,3‐butanediol yield of the novel Klebsiella sp. isolate FSoil 024	17
Medium optimization and subsequent fermentative regulation enabled the scaled‐up production of anti‐tuberculosis drug leads ilamycin‐E1/E2	16
Umbilical cord derived mesenchymal stromal cells in microcarrier based industrial scale culture sustain the immune regulatory functions	16
Liamocin biosynthesis is induced by an autogenous host acid activation in Aureobasidium melanogenum	16
Toehold switch plus signal amplification enables rapid detection	16
Synergistic effects of biological stimuli and flexion induce microcavities promote hypertrophy and inhibit chondrogenesis during in vitro culture of human mesenchymal stem cell aggregates	15
Intermediate product control in cascade reaction for one‐pot production of ε‐caprolactone by Escherichia coli	15
Advancing a rapid, high throughput screening platform for optimization of lentivirus production	15
Redesigning glycolaldehyde synthase for the synthesis of biorefinery feedstock D‐xylulose from C1 compounds	15
Capillary and microchip electrophoresis method development for amino acid monitoring during biopharmaceutical cultivation	15
Outside Front Cover: (Biotechnology Journal 10/2021)	15
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Ribozyme‐processed guide RNA enhances virus‐mediated plant genome editing	14
Outside Front Cover: (Biotechnology Journal 8/2023)	14
Rapid production of multimeric RNA aptamers stabilized by a designed pseudo‐circular structure in E. coli	14
Immobilization of transgenic plant cells towards bioprinting for production of a recombinant biodefense agent	14
Intracellular transformation and disposal mechanisms of magnetosomes in macrophages and cancer cells	14
Serial enrichment of heteroduplex DNA using a MutS‐magnetic bead system	14
Quercetin‐loaded platelets as a potential targeted therapy for glioblastoma multiforme cell line U373‐MG	14
Evaluation of the cytotoxic activity of sorafenib‐loaded camel milk casein nanoparticles against hepatocarcinoma cells	14
Editorial Board: Biotechnology Journal 4/2022	14
Model‐driven design of synthetic N‐terminal coding sequences for regulating gene expression in yeast and bacteria	13
Spinal cord regeneration: A brief overview of the present scenario and a sneak peek into the future	13
Engineering a 3‐dimensional tissue construct with adipose‐derived stem cells for healing bone defect: An ex vivo study with femur head	13
Engineering cannabinoid production in Saccharomyces cerevisiae	13
Through virtual saturation mutagenesis and rational design for superior substrate conversion in engineered d‐amino acid oxidase	13
Potential of butanol production from Thailand marine macroalgae using Clostridium beijerinckii ATCC 10132‐based ABE fermentation	13
Promoting cell growth and characterizing partial symbiotic relationships in the co‐cultivation of green alga Chlamydomonas reinhardtii and Escherichia coli	13
Design and evaluation of a 3D‐printed, lab‐scale perfusion bioreactor for novel biotechnological applications	13
1H NMR‐based process understanding and biochemical marker identification methodology for monitoring CHO cell culture process during commercial‐scale manufacturing	12
E and M SARS‐CoV‐2 membrane protein expression and enrichment with plant lipid droplets	12
Advancements in scaffold for treating ligament injuries; in vitro evaluation	12
Evaluation of expression systems for recombinant protein production in chicken egg bioreactors	12
Developing nanosize carrier systems for Amphotericin‐B: A review on the biomedical application of nanoparticles for the treatment of leishmaniasis and fungal infections	12
Expression and application of aflatoxin degrading enzyme gene in Pichia pastoris	12
Tri‐enzyme fusion of tryptophan halogenase achieves a concise strategy for coenzyme self‐sufficiency and the continuous halogenation of L‐tryptophan	11
In vitro evolution of diagnostic antibodies targeting native antigens in plasma by sandwich flow cytometry	11
Engineering and transcriptome study of Saccharomyces cerevisiae to produce ginsenoside compound K by glycerol	11
Production of D‐tagatose, bioethanol, and microbial protein from the dairy industry by‐product whey powder using an integrated bioprocess	11

Phase separation methods for protein purification: A meta‐analysis of purification performance and cost‐effectiveness	11
Initial cyclic‐di‐GMP upregulation triggers sporadic cellular expansion leading to improved cellular survival	11
Identification of critical process parameters for expansion of clinical grade human Wharton's jelly‐derived mesenchymal stromal cells in stirred‐tank bioreactors	11
Biological autoluminescence enables effective monitoring of yeast cell electroporation	11
Outside Front Cover: (Biotechnology Journal 4/2024)	11
Live‐cell imaging of genomic loci with Cas9 variants	11
Real‐Time Adaptive Inline Acidification Enhances Continuous pH Control for Viral Inactivation	10
Engineering Saccharomyces boulardii for Probiotic Supplementation of l‐Ergothioneine	10
A Hybrid Model Simulating Multi‐Stage Continuous Fermentation of Saccharomyces cerevisiae	10
Adapting microalgae‐based strategies for sustainable green cities	10
Short‐chain alcohols inactivate an immobilized industrial lipase through two different mechanisms	10
Characterization of the Ubiquitin‐Modified Proteome of Recombinant Chinese Hamster Ovary Cells in Response to Endoplasmic Reticulum Stress	10
Multifunctional PAMAM Dendrimers Carrying SAHA, 5‐FU, and a Therapeutic Gene for Targeted Co‐Delivery Toward Colorectal Cancer Cells	10
Visualize PIM‐1 Protein Function and Its Interaction With PI3K/Akt/mTOR Pathway Regulated by Its Active Sites Through FRET Biosensors	10
Knockout of Sf‐Caspase‐1 generates apoptosis‐resistant Sf9 cell lines: Implications for baculovirus expression	10
High fidelity one‐pot DNA assembly using orthogonal serine integrases	10
Transcriptional Downregulation of Methanol Metabolism Key Genes During Yeast Death in Engineered Pichia pastoris	10
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Fluid shear force and hydrostatic pressure jointly promote osteogenic differentiation of BMSCs by activating YAP1 and NFAT2	10
Cytosolic protein delivery using ultrasound‐guided vaporization of perfluorocarbon nano‐droplets	10
Recent advances in applying omic technologies for studying acetic acid bacteria in industrial vinegar production: A comprehensive review	9
Enhancing erythromycin production in Saccharopolyspora erythraea through rational engineering and fermentation refinement: A Design‐Build‐Test‐Learn approach	9
Parallel and large‐scale antitumor investigation using stable chemical gradient and heterotypic three‐dimensional tumor coculture in a multi‐layered microfluidic device	9
Establishment of a cell culture platform for human liver organoids and its application for lipid metabolism research	9
Engineering of the start condensation domain with improved N‐decanoyl catalytic activity for daptomycin biosynthesis	9
The prediction model of nitrogen nutrition in cotton canopy leaves based on hyperspectral visible‐near infrared band feature fusion	9
Generation of stable suspension producer cell lines for serum‐free lentivirus production	9
Reducing water uptake into BY‐2 cells by systematically optimizing the cultivation parameters increases product yields achieved by transient expression in plant cell packs	9
Issue Information: Biotechnology Journal 6/2024	9
Improved synthesis of dietary triglycerides by using lipase supported on clay carriers	9
CpPosNeg: A positive‐negative selection strategy allowing multiple cycles of marker‐free engineering of the Chlamydomonas plastome	9
Combined approach of selective and accelerated cloning for microfluidic chip‐based system increases clone specific productivity	9
Amino acid degradation pathway inhibitory by‐products trigger apoptosis in CHO cells	9
Expanding the RNA polymerase biocatalyst solution space for mRNA manufacture	9
Biotransformation of ethylene glycol to glycolic acid by Yarrowia lipolytica: A route for poly(ethylene terephthalate) (PET) upcycling	8
Evaluation of a novel purified and characterized alkaline protease from the extremophile Exiguobacterium alkaliphilum VLP1 as a detergent additive	8
Highly efficient CRISPR‐mediated base editing for the gut Bacteroides spp. with pnCasBS‐CBE	8
Indigenous microalgae strains characterization for a sustainable biodiesel production	8
Dialysis rolled scaffold bioreactor allows extended production of monoclonal antibody with reduced media use	8
F‐actin microfilaments affect the LIPUS‐promoted osteogenic differentiation of BMSCs through TRPM7	8
Development of an in‐line Raman analytical method for commercial‐scale CHO cell culture process monitoring: Influence of measurement channels and batch number on model performance	8
Precision genome editing in plants using gene targeting and prime editing: existing and emerging strategies	8
Fluorescence‐based screening for engineered aldo‐keto reductase KmAKR with improved catalytic performance and extended substrate scope	8
Proteomics Reveals Distinctive Host Cell Protein Expression Patterns in Fed‐Batch and Perfusion Cell Culture Processes	8
A precise targeting of Staphylococcus aureus with phage RBP‐decorated antibiotic‐loaded nanoparticles	8
Efficient production of glutathione in Saccharomyces cerevisiae via a synthetic isozyme system	8
High‐efficiency bioconversion of phytosterol to bisnoralcohol by metabolically engineered Mycobacterium neoaurum in a micro‐emulsion system	8
A 3D bioprinted antibacterial hydrogel dressing of gelatin/sodium alginate loaded with ciprofloxacin hydrochloride	8
Truncation of a novel C‐terminal domain of a β‐glucanase improves its thermal stability and specific activity	8
Scaling strategy for cell and gene therapy bioreactors based on turbulent parameters	8
Transient ammonia stress on Chinese hamster ovary (CHO) cells yield alterations to alanine metabolism and IgG glycosylation profiles	8
Studying the impact of cell age on the yeast growth behaviour ofSaccharomyces pastorianusvar.carlsbergensisby magnetic separation	8
Targeting metabolic driving and minimization of by‐products synthesis for high‐yield production of D‐pantothenate in Escherichia coli	8
Feeding strategies for Sporosarcina pasteurii cultivation unlock more efficient production of ureolytic biomass for MICP	8
Efficient Conversion of Nicotinamide Mononucleotide Based on Elucidation of the Limitations in Multienzyme Cascade Reactions	8
Enhancement of rAAV titers via inhibition of the interferon signaling cascade in transfected HEK293 suspension cultures	8
Overexpression of Geranylgeranyl Diphosphate Synthase and Cyclase Enhances Pleuromutilin Production in Clitopilus Passeckerianus T6	8
State of the art of bone biomaterials and their interactions with stem cells: Current state and future directions	8
Biomedical application of microalgal‐biomaterials hybrid system	8
Molecular design of controllable recombinant adeno‐associated virus (AAV) expression systems for enhanced vector production	8
Enhanced production of difficult‐to‐express proteins through knocking down rnpA gene expression	8
Experimental study of the topological flow transformations in an aerial vortex bioreactor with a floating washer	8
CRISPR/Cas9‐mediated knockout of a prolyl‐4‐hydroxylase subfamily in Nicotiana benthamiana using DsRed2 for plant selection	7
CRISPR/Cas9 mediated targeted mutagenesis of LIGULELESS‐1 in sorghum provides a rapidly scorable phenotype by altering leaf inclination angle	7
A high‐fidelity, dual site‐specific integration system in CHO cells by a Bxb1 recombinase	7
Standardized approach for accurate and reliable model development of ion‐exchange chromatography based on parameter‐by‐parameter method and consideration of extra‐column effects	7
Fabrication of a decellularized liver matrix–based hepatic patch for the repair of CCl4‐induced liver injury	7
The choice of ultra‐low attachment plates impacts primary human and primary canine hepatocyte spheroid formation, phenotypes, and function	7
Bioinspired scaffolds based on aligned polyurethane nanofibers mimic tendon and ligament fascicles	7
A generic pump‐free organ‐on‐a‐chip platform for assessment of intestinal drug absorption	7
Issue Information: Biotechnology Journal 3/2025	7
Green and ecofriendly biosynthesis of selenium nanoparticles using Urtica dioica (stinging nettle) leaf extract: Antimicrobial and anticancer activity	7
The role of intermolecular interactions on monoclonal antibody filtration through virus removal membranes	7
Editorial Board: Biotechnology Journal 12/2021	7
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Correction to “3D artificial sebocyte glands from inertial focusing effect for facile and flexible analysis of light damage and drug screening”	7
Depolymerization of the polyester–polyurethane by amidase GatA250 and enhancing the production of 4,4′‐methylenedianiline with cutinase LCC	7
An engineered microfluidic blood‐brain barrier model to evaluate the anti‐metastatic activity of β‐boswellic acid	7
Biological potential of polysaccharides extracted from Nostoc colonies for film production –  Physical and biological properties	7
A closed 3D printed microfluidic device for automated growth and differentiation of cerebral organoids from single‐cell suspension	7

CRISPR/Cas9‐based genome engineering in the filamentous fungus Rhizopus oryzae and its application to L‐lactic acid production	7
Massively parallel DNA target capture using long adapter single stranded oligonucleotide (LASSO) probes assembled through a novel DNA recombinase mediated methodology	7
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Development of the IMP biosensor for rapid and stable analysis of IMP concentrations in fermentation broth	7
Time‐Resolved Hierarchical Modeling Highlights Metabolites Influencing Productivity and Cell Death in Chinese Hamster Ovary Cells	7
Multiomics approaches and genetic engineering of metabolism for improved biorefinery and wastewater treatment in microalgae	7
Rational design and experimental evaluation of peptide ligands for the purification of adeno‐associated viruses via affinity chromatography	7
CRISPRa/i with Adaptive Single Guide Assisted Regulation DNA (ASGARD) mediated control of Chlorella sorokiniana to enhance lipid and protein production	6
Genome‐Scale Modeling of CHO Cells Unravel the Critical Role of Asparagine in Cell Culture Feed Media	6
Application of adaptive laboratory evolution to improve the tolerance of Rhodotorula strain to methanol in crude glycerol and development of an effective method for cell lysis	6
Assessing the predictive capabilities of design heuristics and coarse‐grain simulation toward understanding and optimizing site‐specific covalent immobilization of β‐lactamase	6
3D artificial sebocyte glands from inertial focusing effect for facile and flexible analysis of light damage and drug screening	6
A tailored LNA clamping design principle: Efficient, economized, specific and ultrasensitive for the detection of point mutations	6
In vitro acne disease model from inertial focusing effect for studying the interactions between sebocyte glands and macrophages	6
Recombineering facilitates the discovery of natural product biosynthetic pathways in Pseudomonas parafulva	6
ER stress‐induced transcriptional response reveals tolerance genes in yeast	6
In‐silico selection employing rigid docking and molecular dynamic simulation in selecting DNA aptamers against androgen receptor	6
Deletion analysis of Pichia pastoris alcohol dehydrogenase 2 (ADH2) promoter and development of synthetic promoters	6
BMP‐2/TGF‐β1 gene insertion into ligament‐derived stem cells sheet promotes tendon–bone healing in a mouse	6
Improvement of the performance of anticancer peptides using a drug repositioning pipeline	6
Engineering of TEV protease variants with redesigned substrate specificity	6
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Regeneration of Capto Core 700 resin through high throughput and laboratory scale studies and impact on production of a SARS‐CoV‐2 vaccine candidate	6
High yield sterile filtration process for highly concentrated lentiviral vectors	6
Sequence mining yields 18 phloretin C‐glycosyltransferases from plants for the efficient biocatalytic synthesis of nothofagin and phloretin‐di‐C‐glycoside	6
Biosynthesis of the antioxidant γ‐glutamyl‐cysteine with engineered Yarrowia lipolytica	6
Reactor control system in bacterial co‐culture based on fluorescent proteins using an Arduino‐based home‐made device	6
Microbioreactors for nutrient‐controlled microbial cultures: Bridging the gap between bioprocess development and industrial use	6
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Virus inactivation at moderately low pH varies with virus and buffer properties	5
Encapsulation of Pseudomonas aeruginosa elastase inside the P22 virus‐like particle for controlling enzyme–substrate interactions	5
Integrated nucleic acid purification technology based on amino‐modified centrifugal microfluidic chip	5
Outside Front Cover: (Biotechnology Journal 9/2023)	5
Bicarbonate concentration influences carbon utilization rates and biochemical profiles of freshwater and marine microalgae	5
Editorial Board: Biotechnology Journal 5/2023	5
Concentration‐Dependent Effects of MXene Nanocomposite‐Loaded Carboxymethyl Cellulose on Wound Healing	5
Extraction, separation and purification of fatty acid ethyl esters for biodiesel and DHA from Thraustochytrid biomass	5
A Novel and Simplified Anion Exchange Flow‐Through Polishing Approach for the Separation of Full From Empty Adeno‐Associated Virus Capsids	5
Aptabinding of tumor necrosis factor‐α (TNFα) inhibits its proinflammatory effects and alleviates islet inflammation	5
Infectious clone of a contemporary Tembusu virus and replicons expressing reporter genes or heterologous antigens from poultry viruses	5
Outside Front Cover: (Biotechnology Journal 3/2023)	5
Human umbilical cord‐derived mesenchymal stem cells affect urea synthesis and the cell apoptosis of human induced hepatocytes by secreting IL‐6 in a serum‐free co‐culture system	5
Outside Back Cover: (Biotechnology Journal 11/2023)	5
An ultrasonic biosample disruptor with two triangular teeth on its radiation face	5
Editorial: CRISPR and alternative approaches	5
Microfabricated dynamic brain organoid cocultures to assess the effects of surface geometry on assembloid formation	5
The evaluation of biotechnological potential of Gp144, the key molecule of natural predator bacteriophage K in Staphylococcus aureus hunting mechanism	5
Optimization of culture conditions for human bone marrow‐derived mesenchymal stromal cell expansion in macrocarrier‐based Tide Motion system	5
A Competition‐Based Strategy for the Isolation of an Anti‐Idiotypic Blocking Module and Fine‐Tuning for Conditional Activation of a Therapeutic Antibody	5
An Experimental and Modeling Approach to Study Tangential Flow Filtration Performance for mRNA Drug Substance Purification	5
Genome‐Scale Metabolic Modeling of Halomonas elongata 153B Explains Polyhydroxyalkanoate and Ectoine Biosynthesis in Hypersaline Environments	5
Editorial Board: Biotechnology Journal 5/2022	5
Outside Front Cover: (Biotechnology Journal 8/2022)	5
Studying the impact of cell age on the yeast growth behavior of Saccharomyces pastorianus var. carlsbergensis by magnetic separation	5
Outside Front Cover: (Biotechnology Journal 10/2022)	5
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Bio‐based polymers containing traditional medicinal fillers for wound healing applications – An evaluation of neoteric development and future perspectives	5
Editorial Board: Biotechnology Journal 9/2022	5
Use of tRNA gene barriers improves stability of transgene expression in CHO cells	5
Inducible transgene expression in CHO cells using an artificial transcriptional activator with estrogen‐binding domain	5
Outside Front Cover: (Biotechnology Journal 10/2023)	5
Considerations Regarding High Oil Density Bioreactor‐Scale Fermentations of Yarrowia lipolytica Using CFD Modeling and Experimental Validation	5
25SrRNA Methyltransferase CgBMT5 From Candida glycerinogenes Improves Tolerance and Fermentation Performance of Saccharomyces cerevisiae and Yarrowia lipolytica From Undetoxified	5
Lactobacillus reuteri Assists Engineered Bacteria That Target Tumors to Release PD‐L1nb to Mitigate the Adverse Effects of Breast Cancer Immunotherapy	5
Feasibility and performance of cross‐clone Raman calibration models in CHO cultivation	5
Outside Back Cover: (Biotechnology Journal 6/2023)	5
Meta‐fermentation system with a mixed culture for the production of optically pure l‐lactic acid can be reconstructed using the minimum isolates with a simplified pH control strategy	5
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