New Biotechnology

Article	Citations
Eliminating OFF-frame clones in randomized gene libraries: An improved split β-lactamase enrichment system	258
Enzymatic degradation of biopolyesters as a key strategy for bioplastic circularity	123
Production of bacterial cellulose by Komagataeibacter nataicola NRIC 0616 on various culture media	72
Plant-based production of oral vaccines for sustainable fish aquaculture	62
Microbial synthesis of cell penetrating peptides to facilitate the active principle penetration in cosmetics	61
Basic plant research in service of crop breeding – the Application Laboratory for Agricultural Research in Czech Republic	57
Identification and biosynthesis of an autoinducing cyclopeptide from Clostridium acetobutylicum that regulates solvent biosynthesis and cell morphology	52
Advancing fracture repair: gelatin-starch adhesive technique	52
Structure-function landscape models: a new avenue towards the precision engineering of microbial consortia	40
Co-liquefaction of oleaginous yeast and algal biomass: strategic enhancement of bio-crude oil productivity	36
MutS-mediated rapid and cost-effective error correction in in vitro DNA synthesis	33
Whole-cell bacterial biosensors for detection of structurally-diverse chemicals	33
Successful combination of lactic acid bacteria and yeast fermentation and enzymatic treatment to re-cycle industrial bread by-products for bread making	32
OncoFlow: A multiplexed microfluidic platform for personalized drug sensitivity assessment	31
3D printing: Economical and supply chain independent single-use plasticware for cell culture	30
A multi-omics study to boost continuous bolaform sophorolipid production	30
EBRC: Enhancing bioeconomy through research and communication	26
A computational-guided framework and versatile assembly method for genome-wide CRISPRi downregulation in metabolic engineering studies	25
A unique class I polyhydroxyalkanoate synthase (PhaC) from Brevundimonas sp. KH11J01 exists as a functional trimer: A comparative study with PhaC from Cupriavidus necator H16	25
Optimisation of rhamnolipids production using response surface methodology	24
Improving CRISPR-Cas9 mediated genome integration in interspecific hybrid yeasts	24
Recombinant production of a killer yeast toxin in Pichia pastoris and Bacillus subtilis	23
Simultaneous saccharification and fermentation for D-lactic acid production at high temperature by a thermally-adapted homolactogenic Escherichia coli strain	23
Recombinant production of bovine serum albumin (BSA) in yeast: optimization and enhanced blocking capacity in immunoassays	23
Repurposing starchy potato washing slurries and footwear dust in the development of 3D bioplastic packaging - a circular economy approach	21

Sustainable solutions: maximizing resources using agro-industrial by-products for dairy products fortification	21
Phenoplate: An innovative method for assessing interacting effects of temperature and light on non-photochemical quenching in microalgae under chemical stress	21
Balancing act: improving the environmental and economic performance of industrial-scale basic oxygen furnace gas fermentation for isopropyl alcohol production	21
Assessing the potential of olive mill solid waste as feedstock for methane and volatile fatty acids production via anaerobic bioprocesses	20
Automating the design-build-test-learn cycle towards next-generation bacterial cell factories	19
Site-directed neutralizing antibodies targeting structural sites on SARS-CoV-2 spike protein	19
Formation of biologically influenced palladium microstructures by Desulfovibrio desulfuricans and Desulfovibrio ferrophilus IS5	18
A COST Action on microbial responses to low pH: Developing links and sharing resources across the academic-industrial divide	17
Is human oversight to AI systems still possible?	17
Protein coding regions prediction by fusing DNA shape features	17
Post-translational secretion stress regulation in Bacillus subtilis is controlled by intra- and extracellular proteases	17
Co-culture platform for tuning of cancer receptor density allows for evaluation of bispecific immune cell engagers	17
Opinion: Independent third-party entities as a model for validation of commercial antibodies	17
Discovery of a new class of bacterial heme-containing C C cleaving oxygenases	16
Microbial conversion of waste algal biomass to zeaxanthin by using Cellulophaga omnivescoria MSK1 strain	16
Downstream process for the purification of biotechnologically produced D-DIBOA	16
The evolution of reproductive mode in the Carassius auratus complex	16
Biotechnological production of valuable monomers and biopolymers from textile waste blends	16
Ene reductase catalysed formation of chiral carbon(sp3)-sulfur (II) centres	15
Human biological system mimic nano-complexation for pharmaceuticals/cosmetics/functional foods	15
Strategies for the lipidation of bioactive molecules: chemo-enzymatic synthesis of epoxidized fatty acids and their functionalization	15
Identification of halophilic phage genomes from a solar saltern through shotgun sequencing: a metagenomic study	15
Pilot-scale production of mcl-PHA by Pseudomonas citronellolis using acetic acid as the sole carbon source	14
Efficient surface display of single-chain variable fragments against tumor necrosis factor α on engineered probiotic Saccharomyces boulardii and its application in alleviating intestinal inflammation	14
Highly specific metal-binding peptides for selective resource recovery from spent lithium-ion batteries	14
Modifying flavor profiles of Saccharomyces spp. for industrial brewing using FIND-IT, a non-GMO approach for metabolic engineering of yeast	14
Nucleic acid aptamers protect against lead (Pb(II)) toxicity	14
Engineering of NAD-dependent dehydrogenases to change cofactor specificity towards NMN	14
Insights into the effects of anthropogenic activities on oil reservoir microbiome and metabolic potential	14
Enhancing sequestration of heavy metals using exopolysaccharides produced by cyanobacteria grown at ultra-high CO2 levels	14
CO2 and H2-based bio-hexanol production process development with an adapted strain of Clostridium carboxidivorans	14
Extraction and properties of protein extracted from fungal fermentations	13
From the discovery of novel enzymes to their synthetic application: investigating and harnessing natural biodiversity toward efficient and selective bioprocesses	13
Enhancing enzymatic rhamnolipid synthesis: comparative analysis of sustainable energy sources	13
Arsenic removal process from acid mine drainage using bacteria	13
A search for new compounds for candidosis treatment – screening of Antarctic bacteria from the IBA collection	13
Synthetic biology of acetogenic bacteria for sustainable C1 gas bioconversion	13
Beyond iron: harnessing siderophores for the comprehensive mobilization of essential soil elements	12
A Nakaseomyces glabratus CRISPRi platform to study the mode of action of novel antifungals	12
Development of antibodies against the notch ligand Delta-Like-1 by phage display with activity against breast cancer cells	12
Physiology of ethanol-grown Cyberlindnera jadinii: towards sustainable protein production	12
Monitoring metagenomes using long-read nanopore sequencing	12
Fucoxanthin and docosahexaenoic acid production by cold-adapted Tisochrysis lutea	12
Biotechnological potential of amphisin produced on black cumin oilseed cake	12
Establishment of an RNA-based transient expression system in the green alga Chlamydomonas reinhardtii	12
Determination of the oxygen mass transfer coefficients in a bioreactor with mechanical mixing for Poly(3-Hydroxybutyrate) production	12
Earth: Extinguishing anthropogenic risks through harmonization	11
A fattening factor to quantify the accumulation ability of microorganisms under N-starvation	11
Biostimulation effect of different amendments on Cr(VI) recovering microbial community	11
Knock-out of the major regulator Flo8 in Komagataella phaffii results in unique host strain performance for methanol-free recombinant protein production	11

Systematic identification of genomic hotspots for high-yield protein production in CHO cells	11
Immobilization of His6-tagged amine transaminases in microreactors using functionalized nonwoven nanofiber membranes	11
High-throughput screening for heterotrophic growth in microalgae using the Biolog Plate assay	11
Influence of feedstock source on the development of polyhydroxyalkanoates-producing mixed microbial cultures in continuously stirred tank reactors	10
A cell-free high throughput assay for assessment of SARS-CoV-2 neutralizing antibodies	10
Exploration and structure-based engineering of alkenal double bond reductases catalyzing the Cα Cβ double bond reduction of coniferaldehyde	10
Biodegradable zein/PEG nanofibers incorporated with natural antimicrobial compounds for eco-friendly food packaging	10
Energy-efficient clostridial butanol production via histidine kinase regulation to improve strain tolerance and process robustness	9
Optimizing ferritin nanoparticle production for enhanced antigen presentation in vaccine development	9
Bacterial microcompartments: the new biotechnological tool for maximizing catabolic pathway efficiency in microorganisms	9
Valorisation of wastewater treatment sewage scum through the production of biofuels	9
Utilising a genetically encoded biosensor for in-line monitoring of H2O2 generation during methanol assimilation in the yeast Komagataella phaffii	9
Towards a fungal-based colorful future: upscale of colorant production from several fungal species isolated from Dutch parks	9
Development of chemically defined, DMSO-free cryopreservation media for mammalian cells	8
De novo production of valuable aromatic aldehydes and aldehyde-derived products by engineered solvent-tolerant and oxidation-deficient Pseudomonas taiwanensis VLB120	8
Conversion of food processing residues into sustainable soil improvers by using enzymatic hydrolysis	8
Intensified butanol production through optimisation of integrated membrane bioreactor operation	8
Evaluation and optimization of CO2 capture and biomass valorization into value-added products by the green non-sulfur bacteria Chloroflexus aurantiacus	8
Bacterial cell immobilization using the miniaturized adhesive protein AtaA for efficient bioprocesses	7
Valorization of prosecco wine lees for sustainable polyhydroxyalkanoates production by Cupriavidus necator DSM 545 and Hydrogenophaga pseudoflava DSM 1034	7
Analysis of the molecular basis for the non-amylolytic and non-proteolytic nature of Aspergillus vadensis CBS 113365	7
3-hydroxypropionic acid production in engineered Cupriavidus necator H16	7
Valorization of bioethanol by-products to produce unspecific peroxygenase with Agrocybe aegerita: Technological and proteomic perspectives	7
In pursuit of a minimal CHO genome: Establishment of large-scale genome deletions	7
In vivo evolution of lactic acid hyper-tolerant Clostridium thermocellum	7
Exploring the importance of palmitoylation: alkyl chain length of lipidated proteins affects their interaction with lipid membranes and vesicular transport	7
Enzymatic recycling of polyhydroxyalkanoate (PHA) bioplastics using PHA depolymerases	7
An approach to rapid distributed manufacturing of broad spectrum anti-viral griffithsin using cell-free systems to mitigate pandemics	7
XPORT ENTRAP: A droplet microfluidic platform for enhanced DNA transfer between microbial species	7
Upgraded activity assays for PET-degrading enzymes	7
Discovering novel bacterial biosynthetic potential from draft genomic data	6
The pretreatment of sugar, calcium and vitamin C to enhance the color, texture and flavor properties of frozen avocado	6
Impact of standardization in tissue processing: the performance of different fixatives	6
Comparison of alpha-glucuronidase production by E. coli HMS174(DE3) and BL21(DE3)	6
Treatment of real oilfield produced wastewater by ionizing radiation: disinfection and changes in bacterial community and predictive function	6
Development of integrated biofoundry for productions of fuels and chemicals from CO2 and unutilized biomass	6
Catabolism and production of sugar acids by Corynebacterium glutamicum	6
Anti-biofilm molecules from Antarctic marine bacteria as weapons against microbial infections	6
Improved taxadiene production by optimizing DXS expression and fusing short-chain prenyltransferases	6
Bioanalytical technologies for equitable women’s healthcare: development of ultrasensitive assays and bioinspired nanomaterials for biomarker-based diagnostics	6
A versatile odor detection system based on automatically trained rats for chemical sensing	6
Carotenoids-based biohybrid perovskite solar cells mimicking natural photosystem.	6
Unlocking the potential of bioproducts from waste streams for soil restoration	6
Fungal G-protein coupled receptors (GPCRs): potential novel drug targets?	6
A counter-selection system exploiting phenotypic heterogeneity for improved production of glutathione from yeast	6
Semi-continuous cultivation of EPS-producing marine cyanobacteria: A green biotechnology to remove dissolved metals obtaining metal-organic materials	5
Bioinformatics based discovery of new keratinases in protease family M36	5
Antibody-oligonucleotide conjugation based on cyanine dyes as cross-linking reagents	5
Unraveling the proteomics landscape of medium composition in the model biodesulfurization strain Rhodococcus qingshengii IGTS8	5
Multi-enzymatic approaches involving engineered D-amino acid dehydrogenases for the stereoselective synthesis of D-phenylalanines	5
Autotrophic adaptive laboratory evolution of the acetogen Clostridium autoethanogenum delivers the gas-fermenting strain LAbrini with superior growth, products, and robustness	5
Development of a bioprocess for the production of a herbicidal sugar as sustainable alternative to glyphosate	5
Plastics active enzymes for polyolefine and polyester degradation and derivatization	5
C-glycosyltransferase discovery and engineering for the synthesis of bioactive glycosides	5
Exploring yeast diversity for ethanol-fueled industrial biotechnology	5
N-arylated 1,4-diazabicyclo[2.2.2]octane quaternary ammonium salts as a new approach for thiol and selenol luminescent probes design	5
Transcription factor-based biosensors for detection of naturally occurring phenolic acids	5
Significance of poly(ethylene terephthalate) (PET) substrate crystallinity on enzymatic degradation	5
Human milk oligosaccharides: high-yield production of a new food ingredient	5
Green physical processes for the extraction of organic dye betacyanin from dragon fruit (Hylocereus purpusii) peels	5
Computer-aided characterization and evaluation of enzymes active sites	5
From methanol to medicine: sustainable bioproduction of polyketides	5
Investigating and comparing dihydrogen thresholds and consumption kinetics of different methanogenic species	5
Surface layer structure of the periodontal health-associated bacterium Tannerella serpentiformis	5
Engineering synthetic metabolons using self-assembled protein scaffolds	5
Improving 3-hydroxypropionic acid production in E. coli by in silico prediction of new metabolic targets	4
Influence of biochar on biohydrogen production in dark fermentation	4
Getting a grip on oxygen transfer in bioreactors: automated kLa determination using the software Lucullus®	4
Biosynthesis of biodegradable plastic, poly(3HB-co-3HP), using methane as sole carbon source in metabolically engineered type II methanotroph, Methylosinus trichosporium OB3b	4
Precision fermentation and downstream processing of fungal colorants	4
Efficient full-length IgG secretion and sorting from single yeast clones in droplet picoreactors	4
Upcycling cotton textile waste into lactic acid by chemo-mechanical pretreatment, separate hydrolysis and fermentation with engineered Saccharomyces cerevisiae strain	4
Modeling of a bioreactor with immobilized laccase for antibiotic removal considering the effects of diffusion-convection-reaction	4
Evaluation of metallurgical dust recycling applying concepts of bioleaching	4
The Jo-In protein welding system is a relevant tool to create CBM-containing plant cell wall degrading enzymes	4
Metabolic regulation by the chromatin remodeler Ino80 under stress conditions and improvement of yeast stress tolerance for efficient bioproduction	4
Lipopeptide production from xylose and its action in rheology modification for potential industrial application	4
Effect of the stringent response on amylase production and the ribosome profile of Bacillus subtilis	4
Transgenesis in pea (Pisum sativum L.) and flax (Linum usitatissimum L.): Agritec´s experience	4
Bioactive steroids from cortisone bioconversion: antioxidant properties in ocular model	4

Cultivation optimization promotes ginsenoside and universal triterpenoid production by engineered yeast	4
Functional characterization of top-down community assembly in a simple environment	4
Utilising CO2 as a feedstock for renewable chemicals: biocatalytic production of L-lactic acid from CO2 and ethanol	4
Isolation and characterization of novel proteins from octopus	4
Improving the efficiency of chemical production from one-carbon substrates in Komagataella phaffii through enzyme and metabolic engineering	3
Bacillus thuringiensis strain with the transcriptional fusion phaR-GFP and Acetyl CoA-GFP promotor regions to assess bioplastic accumulation	3
New vectors for urea-inducible recombinant protein production	3
Soft sensor for viable cell counting by measuring dynamic oxygen uptake rate	3
Development of an efficient, ready to use, blood platelet-release device based on two new flow regime parameters: The periodic hydrodynamic loading and the shear stress accumulation.	3
Cell-free synthesis of human norovirus capsid protein VP1	3
Pairwise residues coevolution of Candida antarctica lipase B for efficient synthesis of δ-dodecalactone	3
Multifunctional bioplastic coating derived from starch-rich potato washing slurries and tomato pomace for components of footwear	3
Diversifying bioactive natural products: prenylation of plant phenolics and indole derivatives by fungal prenyltransferases	3
Production of nicotinamide mononucleotide by high cell density culture of metabolically engineered Escherichia coli	3
The microalgal sector in Europe: Towards a sustainable bioeconomy	3
ReViTA: A novel in vitro transcription system to study gene regulation	3
Applicability of a laccase from the eucalypt wood endophytic fungus Hormonema sp. CECT-13092 for advanced bioethanol production	3
Upcycling plastic waste: microbial synthesis of bulk chemicals from poly(ethylene terephthalate) (PET)	3
Exosome-like nanoparticles from Arbutus unedo L mitigate LPS-induced inflammation via JAK-STAT inactivation	3
Production of a plant-based vaccine candidate against COVID-19 through molecular pharming	3
Engineering Pseudomonas putida for bioproduction of alkylamines	3
Targeting enzymes for plastic degradation: developing Affimers as novel plastic-binding modules	3
Enzymatic production of (S)-citronellol from citral using an Old Yellow Enzyme and a short-chain alcohol dehydrogenase	3
Enhancing 2,3-butanediol and acetoin production from brewer's spent grain hemicellulosic hydrolysate through bacterial co-cultivation	3
Gene essentiality in the solventogenic Clostridium acetobutylicum DSM 792	3
Biotechnological recovery of critical raw materials from end-of-life lithium-ion batteries	3
Utilization of lactose and whey permeate for the sustainable production of polyhydroxyalkanoates by Hydrogenophaga pseudoflava DSM1034	2
Recovery of C6 carboxylate from CO₂-based microbial electrosynthesis	2
Virtual screening of bioactive peptides with antimicrobial activity targeting carbonic anhydrase II from Escherichia coli	2
Antifungal activity of biosurfactants against plant pathogenic fungi	2
Immobilization of Dietzia sp. KDB1 bacteria on electrospun fiber mats for indigo reduction	2
Targeted mutagenesis in plants using Beet curly top virus for efficient delivery of CRISPR/Cas12a components	2
A gradient phage-assisted continuous evolution method for screening suppressor tRNAs in Escherichia coli	2
Biotechnology applied to soybean cultivation: the use of microorganisms to increase drought tolerance	2
Fungal waste treats water waste: enzymatic and non-enzymatic removal of organic micropollutants with spent mushroom substrate of Agaricus bisporus	2
Serotonin overproduction in Saccharomyces cerevisiae: a metabolic engineering approach	2
A systems-view and optimization of bacteria with synthetic reductive glycine pathway for C1-assimilation	2
Unlocking the power of Moringa oleifera: a surprising synergy with cereal-based products	2
Caffeine-regulated molecular switches for functional control of CAR T cells	2
Editorial Board	2
Shedding light on the black box of a neural network used to detect prostate cancer in whole slide images by occlusion-based explainability	2
Development of a self-powered electric and UV disinfection system based on a microbial electrochemical system	2
Advancements in flavonoid glycosylation: unlocking bioproduction potential with Escherichia coli W as novel platform for large-scale production	2
Gramicidin S production through exclusive use of agrifood byproducts as nutritional sources	2
Influence of whey protein on black soybean anthocyanins’ thermostability and inhibitory effect toward xanthine oxidase	2
Plant expression systems as an economical alternative for the production of iELISA coating antigen AHSV VP7	2
Editorial Board	2
Production and characterization of novel Anti-HIV Fc-fusion proteins in plant-based systems: Nicotiana benthamiana & tobacco BY-2 cell suspension	1
Domain shifts in dermoscopic skin cancer datasets: Evaluation of essential limitations for clinical translation	1
SARS-CoV2 neutralizing activity of ozone on porous and non-porous materials	1
Identification and expression of novel polyester-degrading enzymes for application on mixed plastic-containing waste	1
P. pastoris (K. phaffi) perfusion processes: evaluation of cell retention techniques and integrated purification strategies	1
Optimizing RuBisCO catalytic activity through active-site loop stabilization	1
Reducing cellulases production cost through systematically optimizing submerged fermentation of Trichoderma reesei	1
In vitro protein synthesis versus E. coli expression - a comparison using the acetate transporter SatP	1
Glucose to nitrogen ratio effect for phycocyanin productivity under hetero- and mixotrophic conditions with the microalga Galdieria sulphuraria	1
An engineered SARS-CoV-2 receptor-binding domain produced in Pichia pastoris as a candidate vaccine antigen	1
Phytoremediation of diclofenac using the Green Liver System: Macrophyte screening to system optimization	1
Virus inactivation using an electrically conducting virus filter in biopharmaceutical manufacturing process	1
Strategic improvement of Shewanella oneidensis for biocatalysis: Approach to media refinement and scalable application in a microbial electrochemical system	1
Enzyme design and engineering for efficient biosynthesis of high-value compounds	1
Exploring Nordic microalgae as a potential novel source of antioxidant and bioactive compounds	1
A hitchhiker’s guide to stable co-cultures for industrial biotechnology	1
Going beyond the limit: engineering mRNA decay and protein translation initiation enhances recombinant protein secretion	1
Explainability and causability for artificial intelligence-supported medical image analysis in the context of the European In Vitro Diagnostic Regulation	1
The PAR promoter expression system: Modified lac promoters for controlled recombinant protein production in Escherichia coli	1
Conversion of syngas-derived C2 carbon sources to C2+ biochemicals by microbial cell factories	1
Effects of zingerone nanoparticles on anti-angiogenesis in vitro and in vivo	1
Advancing biobased product development from diverse bioresources through customized designer bioactivities	1
Continuous VFA production from lignocellulosic biomass via an artificial rumen reactor and membrane filtration: the cow as inspiration	1
Applying a transaminase for the sustainable production of 5-hydroxymethylfurfurylamine using cadaverine	1
A hybrid fermentation-catalysis bioprocess strategy to convert food waste into high-value polymer precursors	0
RNA-based SIBR as a tool for post-transcriptional regulation	0
Bubbles and broth: how fermentation broth components can affect mass transfer	0
Quality comparison of recombinant soluble proteins and proteins solubilized from bacterial inclusion bodies	0
Exploring simultaneous production of poly(3-hydroxybutyrate) and exopolysaccharides in cyanobacteria-rich microbiomes	0
Biofuel synthesis from CO2 and CH4 at ambient temperature by coupling chemical and biological system	0
Substituting water by ethylene glycol in MsAct transesterification	0
Modification of magnetite-doped NH2-MIL-100(Fe) by aliphatic C8 or C18 carbon chain with excellent hydrophobicity for effective protein separation and purification	0
ISPRIN: a tool to enable continuous L/L extraction at lab scale for microbial strain- and process development of inhibiting compounds	0
Engineered Pseudomonas putida for customized polyhydroxyalkanoate (PHA) production	0
Biomaterials text mining: A hands-on comparative study of methods on polydioxanone biocompatibility	0
Effect of residence time in continuous photobioreactor on mass and energy balance of microalgal protein production	0
A rapid CAT transformation protocol and nuclear transgene expression tools for metabolic engineering in Cyanidioschyzon merolae 10D	0
Bioinformatics and functional selection of GH77 4-α-glucanotransferases for potato starch modification	0
Silk fibroin production in tobacco plants for medical applications	0
HPC-driven strategies for enzyme discovery and improvement: a focus on plastic degrading enzymes	0
Biodegradation of naphthalene, fluorene, and anthracene by Ochrobactrum intermedium BC1 and Cupriavidus ⁠ taiwanensis LA strains isolated from Saudi Arabia	0
Molecular modeling of thermostable neutral protease (NPr) from Geobacillus sp. DS3 and inhibition by phosphoramidon and N-phosphoryl-L-leucinamide	0
Adaptive laboratory evolution to hypersaline conditions of lactic acid bacteria isolated from seaweed	0
The influence of different carbon sources on growth and single cell oil production in oleaginous yeasts Apiotrichum brassicae and Pichia kudriavzevii	0
Shikimate pathway-dependent catabolism: enabling near-to-maximum production of aromatics	0
A filter based whole-cell sensor design for measuring in bioreactors	0
Laccase production using organic substrates derived from agro-industrial residues and coproducts	0
Utilizing cell wall integrity response in Saccharomyces cerevisiae for enhanced chitin production: genetic engineering and adaptive laboratory evolution strategies	0