OOIR: Observatory of International Research

Papers

(The median citation count of New Biotechnology is 4. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-06-01 to 2025-06-01.)

Article	Citations
Successful combination of lactic acid bacteria and yeast fermentation and enzymatic treatment to re-cycle industrial bread by-products for bread making	222
Eliminating OFF-frame clones in randomized gene libraries: An improved split β-lactamase enrichment system	105
3D printing: Economical and supply chain independent single-use plasticware for cell culture	61
A unique class I polyhydroxyalkanoate synthase (PhaC) from Brevundimonas sp. KH11J01 exists as a functional trimer: A comparative study with PhaC from Cupriavidus necator H16	55
EBRC: Enhancing bioeconomy through research and communication	48
A multi-omics study to boost continuous bolaform sophorolipid production	48
MutS-mediated rapid and cost-effective error correction in in vitro DNA synthesis	47
Formation of biologically influenced palladium microstructures by Desulfovibrio desulfuricans and Desulfovibrio ferrophilus IS5	45
Automating the design-build-test-learn cycle towards next-generation bacterial cell factories	35
Phenoplate: An innovative method for assessing interacting effects of temperature and light on non-photochemical quenching in microalgae under chemical stress	32
Site-directed neutralizing antibodies targeting structural sites on SARS-CoV-2 spike protein	31
Assessing the potential of olive mill solid waste as feedstock for methane and volatile fatty acids production via anaerobic bioprocesses	29
Modifying flavor profiles of Saccharomyces spp. for industrial brewing using FIND-IT, a non-GMO approach for metabolic engineering of yeast	28
Is human oversight to AI systems still possible?	28
Insights into the effects of anthropogenic activities on oil reservoir microbiome and metabolic potential	27
Nucleic acid aptamers protect against lead (Pb(II)) toxicity	26
Discovery of a new class of bacterial heme-containing C C cleaving oxygenases	25
A COST Action on microbial responses to low pH: Developing links and sharing resources across the academic-industrial divide	23
Co-culture platform for tuning of cancer receptor density allows for evaluation of bispecific immune cell engagers	22
Post-translational secretion stress regulation in Bacillus subtilis is controlled by intra- and extracellular proteases	21
Efficient surface display of single-chain variable fragments against tumor necrosis factor α on engineered probiotic Saccharomyces boulardii and its application in alleviating intestinal inflammation	21
Protein coding regions prediction by fusing DNA shape features	21
Knock-out of the major regulator Flo8 in Komagataella phaffii results in unique host strain performance for methanol-free recombinant protein production	20
Pilot-scale production of mcl-PHA by Pseudomonas citronellolis using acetic acid as the sole carbon source	20
Exploration and structure-based engineering of alkenal double bond reductases catalyzing the Cα Cβ double bond reduction of coniferaldehyde	20

Opinion: Independent third-party entities as a model for validation of commercial antibodies	20
Establishment of an RNA-based transient expression system in the green alga Chlamydomonas reinhardtii	19
Fucoxanthin and docosahexaenoic acid production by cold-adapted Tisochrysis lutea	18
Influence of feedstock source on the development of polyhydroxyalkanoates-producing mixed microbial cultures in continuously stirred tank reactors	18
Earth: Extinguishing anthropogenic risks through harmonization	18
High-throughput screening for heterotrophic growth in microalgae using the Biolog Plate assay	17
Development of antibodies against the notch ligand Delta-Like-1 by phage display with activity against breast cancer cells	17
A fattening factor to quantify the accumulation ability of microorganisms under N-starvation	17
Biostimulation effect of different amendments on Cr(VI) recovering microbial community	16
Immobilization of His6-tagged amine transaminases in microreactors using functionalized nonwoven nanofiber membranes	16
A cell-free high throughput assay for assessment of SARS-CoV-2 neutralizing antibodies	16
XPORT ENTRAP: A droplet microfluidic platform for enhanced DNA transfer between microbial species	15
An approach to rapid distributed manufacturing of broad spectrum anti-viral griffithsin using cell-free systems to mitigate pandemics	15
Impact of standardization in tissue processing: the performance of different fixatives	14
In vivo evolution of lactic acid hyper-tolerant Clostridium thermocellum	14
Significance of poly(ethylene terephthalate) (PET) substrate crystallinity on enzymatic degradation	14
Analysis of the molecular basis for the non-amylolytic and non-proteolytic nature of Aspergillus vadensis CBS 113365	14
Valorization of bioethanol by-products to produce unspecific peroxygenase with Agrocybe aegerita: Technological and proteomic perspectives	14
Improved taxadiene production by optimizing DXS expression and fusing short-chain prenyltransferases	14
In pursuit of a minimal CHO genome: Establishment of large-scale genome deletions	14
Porcine circovirus 2 capsid protein produced in N. benthamiana forms virus-like particles that elicit production of virus-neutralizing antibodies in guinea pigs	13
Improving 3-hydroxypropionic acid production in E. coli by in silico prediction of new metabolic targets	13
Bioinformatics based discovery of new keratinases in protease family M36	13
The Jo-In protein welding system is a relevant tool to create CBM-containing plant cell wall degrading enzymes	13
Transcription factor-based biosensors for detection of naturally occurring phenolic acids	13
Semi-continuous cultivation of EPS-producing marine cyanobacteria: A green biotechnology to remove dissolved metals obtaining metal-organic materials	13
Autotrophic adaptive laboratory evolution of the acetogen Clostridium autoethanogenum delivers the gas-fermenting strain LAbrini with superior growth, products, and robustness	13
New vectors for urea-inducible recombinant protein production	12
The microalgal sector in Europe: Towards a sustainable bioeconomy	12
Development of an efficient, ready to use, blood platelet-release device based on two new flow regime parameters: The periodic hydrodynamic loading and the shear stress accumulation.	12
Targeted mutagenesis in plants using Beet curly top virus for efficient delivery of CRISPR/Cas12a components	12
ReViTA: A novel in vitro transcription system to study gene regulation	12
Cultivation optimization promotes ginsenoside and universal triterpenoid production by engineered yeast	12
Plant expression systems as an economical alternative for the production of iELISA coating antigen AHSV VP7	12
Soft sensor for viable cell counting by measuring dynamic oxygen uptake rate	12
Editorial Board	11
Domain shifts in dermoscopic skin cancer datasets: Evaluation of essential limitations for clinical translation	11
A gradient phage-assisted continuous evolution method for screening suppressor tRNAs in Escherichia coli	11
Shedding light on the black box of a neural network used to detect prostate cancer in whole slide images by occlusion-based explainability	11
Production and characterization of novel Anti-HIV Fc-fusion proteins in plant-based systems: Nicotiana benthamiana & tobacco BY-2 cell suspension	11
Editorial Board	11
Explainability and causability for artificial intelligence-supported medical image analysis in the context of the European In Vitro Diagnostic Regulation	11
An engineered SARS-CoV-2 receptor-binding domain produced in Pichia pastoris as a candidate vaccine antigen	11
Establishment of a Cell-Free Multi-Enzyme Cascade for the Synthesis of UDP-GalNAc	10
SARS-CoV2 neutralizing activity of ozone on porous and non-porous materials	10
The PAR promoter expression system: Modified lac promoters for controlled recombinant protein production in Escherichia coli	10
Exploring Nordic microalgae as a potential novel source of antioxidant and bioactive compounds	10
Strategic improvement of Shewanella oneidensis for biocatalysis: Approach to media refinement and scalable application in a microbial electrochemical system	10
Virus inactivation using an electrically conducting virus filter in biopharmaceutical manufacturing process	10
Phytoremediation of diclofenac using the Green Liver System: Macrophyte screening to system optimization	10

Open (non-sterile) cultivations of Debaryomyces hansenii for recombinant protein production combining industrial side-streams with high salt content	9
Deciphering integration loci of CHO manufacturing cell lines using long read nanopore sequencing	9
Enhancing protein productivities in CHO cells through adenosine uptake modulation – Novel insights into cellular growth and productivity regulation	9
Engineering a heterologously expressed fructosyltransferase from Aspergillus oryzae N74 in Komagataella phaffii (Pichia pastoris) for kestose production	9
MiDSystem: A comprehensive online system for de novo assembly and analysis of microbial genomes	9
A unique biochemical reaction pathway towards trehalulose synthesis by an amylosucrase isolated from Deinococcus deserti	9
CaRA – A multi-purpose phage display library for selection of calcium-regulated affinity proteins	8
A new control strategy to improve the mass transfer capacity and reduce air injection costs in raceway reactors	8
Towards polyethylene terephthalate valorisation into PHB using an engineered Comamonas testosteroni strain	8
Comparative genomics reveals the organic acid biosynthesis metabolic pathways among five lactic acid bacterial species isolated from fermented vegetables	7
Provenance of specimen and data – A prerequisite for AI development in computational pathology	7
Accelerostat study in conventional and microfluidic bioreactors to assess the key role of residual glucose in the dimorphic transition of Yarrowia lipolytica in response to environmental stimuli	7
Barley as a production platform for oral vaccines in sustainable fish aquaculture	7
Novel cis-regulatory elements as synthetic promoters to drive recombinant protein expression from the Chlamydomonas reinhardtii nuclear genome	7
Biosynthesis of D-1,2,4-butanetriol promoted by a glucose-xylose dual metabolic channel system in engineered Escherichia coli	7
TILAFeed: A bio-based inventory for circular nutrients management and achieving bioeconomy in future aquaponics	7
Genetic code expansion in E. coli enables production of a functional ‘ready-to-click’ T cell receptor-specific scFv	7
Clinically compatible advances in blood-derived endothelial progenitor cell isolation and reprogramming for translational applications	7
An artificial intelligence approach for identification of microalgae cultures	7
Applicability and information value of biocalorimetry for the monitoring of fungal solid-state fermentation of lignocellulosic agricultural by-products	7
Optimization of genome editing by CRISPR ribonucleoprotein for high efficiency of germline transmission of Sox9 in zebrafish	7
Environmental evaluation of polyhydroxyalkanoates from animal slaughtering waste using Material Input Per Service Unit	7
A novel data-driven model for prediction and adaptive control of pH in raceway reactor for microalgae cultivation	7
A step closer to continuous buffer preparation from solids: Predicting powder compaction and how to prevent it	6
Machine learning reveals novel compound for the improved production of chitooligosaccharides in Escherichia coli	6
Replication-competent influenza virus with a protein-responsive multiplication ability	6
Pre-analytical properties of different respiratory viruses for PCR-based detection: Comparative analysis of sampling devices and sample stabilization solutions	6
Development of Komagataella phaffii as a cell factory for efficient de novo production of β-caryophyllene	6
The stressostat: A novel approach in adaptive laboratory evolution to improve end-product resistance	6
High throughput purification of monoclonal recombinant antibodies using a Protein-A coated membrane plate system	6
Extracellular Vesicles based STAT3 delivery as innovative therapeutic approach to restore STAT3 signaling deficiency	6
Comprehensive rheological analysis of structurally related acetan-like heteroexopolysaccharides from two Kozakia baliensis strains in surfactants and galactomannan blends	6
A novel high-throughput approach for transforming filamentous fungi employing a droplet-based microfluidic platform	6
Elevated CO2 enhances growth and cyanide assimilation in nitrogen-deficient rice: A transcriptome and metabolomic perspective	6
Natural deep eutectic solvents as thermostabilizer for Humicola insolens cutinase	6
Machine translation of standardised medical terminology using natural language processing: A scoping review	6
Impact of the pre-examination phase on multicenter metabolomic studies	6
Novel GH109 enzymes for bioconversion of group A red blood cells to the universal donor group O	5
From food waste to eco-friendly functionalized polymer composites: Investigation of orange peels as active filler	5
Corrigendum to “Manipulating gene expression levels in mammalian cell factories: An outline of synthetic molecular toolboxes to achieve multiplexed control” [New Biotechnol 79 (2024) 1–19]	5
Derivation of splice junction-specific antibodies using a unique hapten targeting strategy and directed evolution	5
Taming CRISPRi: Dynamic range tuning through guide RNA diversion	5
EasyMAP: A user-friendly online platform for analyzing 16S ribosomal DNA sequencing data	5
Electrochemical H2O2 - stat mode as reaction concept to improve the process performance of an unspecific peroxygenase	5
Effective application of immobilized second generation industrial Saccharomyces cerevisiae strain on consolidated bioprocessing	5
Next biotechnological plants for addressing global challenges: The contribution of transgenesis and new breeding techniques	5
A cascade reaction for the synthesis of d-fagomine precursor revisited: Kinetic insight and understanding of the system	5
Chloroplast engineering of the green microalgae Chlamydomonas reinhardtii for the production of HAA, the lipid moiety of rhamnolipid biosurfactants	5
Carbohydrate esterase family 16 contains fungal hemicellulose acetyl esterases (HAEs) with varying specificity	5
A single-chain variable fragment selected against a conformational epitope of a recombinantly produced snake toxin using phage display	4
Editorial Board	4
Artificial intelligence / machine-learning tool for post-market surveillance of in vitro diagnostic assays	4
Improving CRISPR-Cas9 mediated genome integration in interspecific hybrid yeasts	4
Combining the mechanical ball milling of the carbohydrate and the use of low solvent reaction media for the synthesis of fructose fatty acid esters by immobilized lipases	4
Controlled co-immobilisation of proteins via 4′-phosphopantetheine-mediated site-selective covalent linkage	4
Biorefinery of brewery spent grain to obtain bioproducts with high value-added in the market	4
Increasing the sustainability of photoautotrophic microalgae production by minimising freshwater requirements	4
Food and human health applications of edible mushroom by-products	4
Interlaboratory evaluation of quality control methods for circulating cell-free DNA extraction	4
Polyhydroxyalkanoates production from cheese whey under near-seawater salinity conditions	4
Ready-to-use vertical flow paper device for instrument-free room temperature reverse transcription	4
Effect of seawater on the biomass composition of Spirulina produced at a pilot-scale	4
Thauera sp. Sel9, a new bacterial strain for polyhydroxyalkanoates production from volatile fatty acids	4
Approaches for high-throughput quantification of periplasmic recombinant proteins	4
Purple bacteria screening for photoautohydrogenotrophic food production: Are new H2-fed isolates faster and nutritionally better than photoheterotrophically obtained reference species?	4
The promise of explainable deep learning for omics data analysis: Adding new discovery tools to AI	4
Combination of sodium butyrate and decitabine promotes transgene expression in CHO cells via apoptosis inhibition	4
Comparison of process mass intensity (PMI) of continuous and batch manufacturing processes for biologics	4
Identification of lipolytic enzymes using high-throughput single-cell screening and sorting of a metagenomic library	4
BIBBOX, a FAIR toolbox and App Store for life science research	4
Point-of-care diagnosis of invasive non-typhoidal Salmonella enterica in bloodstream infections using immunomagnetic capture and loop-mediated isothermal amplification	4