OOIR: Observatory of International Research

Papers

(The median citation count of New Biotechnology is 4. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-04-01 to 2025-04-01.)

Article	Citations
Split fluorescent protein-mediated multimerization of cell wall binding domain for highly sensitive and selective bacterial detection	206
Porcine circovirus 2 capsid protein produced in N. benthamiana forms virus-like particles that elicit production of virus-neutralizing antibodies in guinea pigs	93
Combining the mechanical ball milling of the carbohydrate and the use of low solvent reaction media for the synthesis of fructose fatty acid esters by immobilized lipases	56
Microfluidic tool for rapid functional characterization of CRISPR complexes	52
Editorial Board	46
3D printing: Economical and supply chain independent single-use plasticware for cell culture	43
Artificial intelligence / machine-learning tool for post-market surveillance of in vitro diagnostic assays	41
A multi-omics study to boost continuous bolaform sophorolipid production	40
Accelerostat study in conventional and microfluidic bioreactors to assess the key role of residual glucose in the dimorphic transition of Yarrowia lipolytica in response to environmental stimuli	36
A novel thermophile β-galactosidase from Thermothielavioides terrestris producing galactooligosaccharides from acid whey	32
An artificial intelligence approach for identification of microalgae cultures	31
Extracellular and intracellular phenol production by microalgae during photoautotrophic batch cultivation	30
Increased accumulation of fatty acids in engineered Saccharomyces cerevisiae by co-overexpression of interorganelle tethering protein and lipases	27
Transcription factor-based biosensors for detection of naturally occurring phenolic acids	26
Droplet digital PCR: A comprehensive tool for genetic analysis and prediction of bispecific antibody assembly during cell line development	26
EBRC: Enhancing bioeconomy through research and communication	25
Effects of municipal waste compost on microbial biodiversity and energy production in terrestrial microbial fuel cells	22
Towards polyethylene terephthalate valorisation into PHB using an engineered Comamonas testosteroni strain	22
Continuous H-B-E fermentation by Clostridium carboxidivorans: CO vs syngas	21
BIBBOX, a FAIR toolbox and App Store for life science research	21
Polyhydroxyalkanoates production from cheese whey under near-seawater salinity conditions	20
The Jo-In protein welding system is a relevant tool to create CBM-containing plant cell wall degrading enzymes	20
Successful combination of lactic acid bacteria and yeast fermentation and enzymatic treatment to re-cycle industrial bread by-products for bread making	20
Novel cis-regulatory elements as synthetic promoters to drive recombinant protein expression from the Chlamydomonas reinhardtii nuclear genome	19
Semi-continuous cultivation of EPS-producing marine cyanobacteria: A green biotechnology to remove dissolved metals obtaining metal-organic materials	19

Bioinformatics based discovery of new keratinases in protease family M36	18
Ready-to-use vertical flow paper device for instrument-free room temperature reverse transcription	17
Applicability and information value of biocalorimetry for the monitoring of fungal solid-state fermentation of lignocellulosic agricultural by-products	17
Combination of sodium butyrate and decitabine promotes transgene expression in CHO cells via apoptosis inhibition	17
Eliminating OFF-frame clones in randomized gene libraries: An improved split β-lactamase enrichment system	16
Improving 3-hydroxypropionic acid production in E. coli by in silico prediction of new metabolic targets	16
Controlled co-immobilisation of proteins via 4′-phosphopantetheine-mediated site-selective covalent linkage	16
CaRA – A multi-purpose phage display library for selection of calcium-regulated affinity proteins	16
Improving CRISPR-Cas9 mediated genome integration in interspecific hybrid yeasts	16
Streamlined life cycle assessment of single use technologies in biopharmaceutical manufacture	15
Towards detection of SARS-CoV-2 RNA in human saliva: A paper-based cell-free toehold switch biosensor with a visual bioluminescent output	15
Genomic mining of Geobacillus stearothermophilus GF16 for xylose production from hemicellulose-rich biomasses using secreted enzymes	15
Cell-based glycoengineering of extracellular vesicles through precise genome editing	14
Poly(3-hydroxybutyrate) production from methane in bubble column bioreactors: Process simulation and design optimization	14
Comparative genomics reveals the organic acid biosynthesis metabolic pathways among five lactic acid bacterial species isolated from fermented vegetables	13
Generation of an anti-idiotypic affibody-based masking domain for conditional activation of EGFR-targeting	13
Assessing the potential of olive mill solid waste as feedstock for methane and volatile fatty acids production via anaerobic bioprocesses	13
Point-of-care diagnosis of invasive non-typhoidal Salmonella enterica in bloodstream infections using immunomagnetic capture and loop-mediated isothermal amplification	13
Genetic code expansion in E. coli enables production of a functional ‘ready-to-click’ T cell receptor-specific scFv	13
Identification of lipolytic enzymes using high-throughput single-cell screening and sorting of a metagenomic library	13
A unique class I polyhydroxyalkanoate synthase (PhaC) from Brevundimonas sp. KH11J01 exists as a functional trimer: A comparative study with PhaC from Cupriavidus necator H16	13
Synthetic activation of yeast stress response improves secretion of recombinant proteins	13
Soft sensor for viable cell counting by measuring dynamic oxygen uptake rate	13
MAGMA: Microbial and Algal Growth Modeling Application	13
Approaches for high-throughput quantification of periplasmic recombinant proteins	12
ReViTA: A novel in vitro transcription system to study gene regulation	12
The origins and success of the EFB Journal New Biotechnology under the leadership of Mike Taussig	12
Using galactitol dehydrogenase coupled with water-forming NADH oxidase for efficient enzymatic synthesis of L-tagatose	11
Manipulating gene expression levels in mammalian cell factories: An outline of synthetic molecular toolboxes to achieve multiplexed control	11
Formation of biologically influenced palladium microstructures by Desulfovibrio desulfuricans and Desulfovibrio ferrophilus IS5	11
Clinically compatible advances in blood-derived endothelial progenitor cell isolation and reprogramming for translational applications	11
Thauera sp. Sel9, a new bacterial strain for polyhydroxyalkanoates production from volatile fatty acids	11
Detection of SARS-CoV-2 antibodies in serum and dried blood spot samples of vaccinated individuals using a sensitive homogeneous proximity extension assay	11
Variation in non-target traits in genetically modified hybrid aspens does not exceed natural variation	11
Provenance of specimen and data – A prerequisite for AI development in computational pathology	10
Development of an efficient, ready to use, blood platelet-release device based on two new flow regime parameters: The periodic hydrodynamic loading and the shear stress accumulation.	10
Environmental evaluation of polyhydroxyalkanoates from animal slaughtering waste using Material Input Per Service Unit	10
Purple bacteria screening for photoautohydrogenotrophic food production: Are new H2-fed isolates faster and nutritionally better than photoheterotrophically obtained reference species?	10
Site-directed neutralizing antibodies targeting structural sites on SARS-CoV-2 spike protein	10
Biorefinery of brewery spent grain to obtain bioproducts with high value-added in the market	10
Automating the design-build-test-learn cycle towards next-generation bacterial cell factories	10
Biosynthesis of D-1,2,4-butanetriol promoted by a glucose-xylose dual metabolic channel system in engineered Escherichia coli	10
Large-scale cultivation of Synechocystis sp. PCC6803 for the production of Poly(3-hydroxybutyrate) and its potential applications in the manufacturing of bulk and medical prototypes	10
Polyethylene and related hydrocarbon polymers (“plastics”) are not biodegradable	10
Is human oversight to AI systems still possible?	9
A novel data-driven model for prediction and adaptive control of pH in raceway reactor for microalgae cultivation	9
The promise of explainable deep learning for omics data analysis: Adding new discovery tools to AI	9
Extracellular Vesicles based STAT3 delivery as innovative therapeutic approach to restore STAT3 signaling deficiency	9
A wide array of lignin-related phenolics are oxidized by an evolved bacterial dye-decolourising peroxidase	9
Rhamnolipids from non-pathogenic Acinetobacter calcoaceticus: Bioreactor-scale production, characterization and wound healing potency	9

TILAFeed: A bio-based inventory for circular nutrients management and achieving bioeconomy in future aquaponics	9
Phenoplate: An innovative method for assessing interacting effects of temperature and light on non-photochemical quenching in microalgae under chemical stress	9
A single-chain variable fragment selected against a conformational epitope of a recombinantly produced snake toxin using phage display	9
Enzymatic synthesis of wet-resistant lignosulfonate-starch adhesives	9
Food and human health applications of edible mushroom by-products	9
Cultivation optimization promotes ginsenoside and universal triterpenoid production by engineered yeast	9
Gallic acid melanin pigment hydrogel as a flexible macromolecule for articular motion sensing	8
Biomaterials text mining: A hands-on comparative study of methods on polydioxanone biocompatibility	8
Micrometric DNA/PEI polyplexes correlate with higher transient gene expression yields in HEK 293 cells	7
Functionalisation of polyhydroxybutyrate for diagnostic uses	7
Elevated CO2 enhances growth and cyanide assimilation in nitrogen-deficient rice: A transcriptome and metabolomic perspective	7
Autophagy and intracellular product degradation genes identified by systems biology analysis reduce aggregation of bispecific antibody in CHO cells	7
Exploiting UPO versatility to transform rutin in more soluble and bioactive products	7
The influence of different carbon sources on growth and single cell oil production in oleaginous yeasts Apiotrichum brassicae and Pichia kudriavzevii	7
A rapid CAT transformation protocol and nuclear transgene expression tools for metabolic engineering in Cyanidioschyzon merolae 10D	7
Barley as a production platform for oral vaccines in sustainable fish aquaculture	7
Plant expression systems as an economical alternative for the production of iELISA coating antigen AHSV VP7	6
Replication-competent influenza virus with a protein-responsive multiplication ability	6
Purification of DNA oligonucleotides to improve hybridization chain reaction performance	6
A COST Action on microbial responses to low pH: Developing links and sharing resources across the academic-industrial divide	6
A novel high-throughput approach for transforming filamentous fungi employing a droplet-based microfluidic platform	6
Protein coding regions prediction by fusing DNA shape features	6
Sustainable production of food grade omega-3 oil using aquatic protists: Reliability and future horizons	6
Incorporation of a repeated polypeptide sequence in therapeutic antibodies as a universal masking procedure: A case study of T cell-engaging bispecific antibodies	6
Co-culture platform for tuning of cancer receptor density allows for evaluation of bispecific immune cell engagers	6
Inefficient transcription is a production bottleneck for artificial therapeutic BiTE® proteins	6
Nucleic acid aptamers protect against lead (Pb(II)) toxicity	5
High throughput purification of monoclonal recombinant antibodies using a Protein-A coated membrane plate system	5
Special issue in memory of Valter Tandoi (IRSA-CNR) – A life-long commitment to environmental biotechnology	5
Effect of residence time in continuous photobioreactor on mass and energy balance of microalgal protein production	5
Pilot-scale production of mcl-PHA by Pseudomonas citronellolis using acetic acid as the sole carbon source	5
The stressostat: A novel approach in adaptive laboratory evolution to improve end-product resistance	5
Quality comparison of recombinant soluble proteins and proteins solubilized from bacterial inclusion bodies	5
Discovery of a new class of bacterial heme-containing C C cleaving oxygenases	5
New vectors for urea-inducible recombinant protein production	5
Corrigendum to bioprocess intensification: Cases that (don’t) work	5
Editorial Board	5
Adaptive laboratory evolution to hypersaline conditions of lactic acid bacteria isolated from seaweed	5
Interlaboratory evaluation of quality control methods for circulating cell-free DNA extraction	5
Harnessing nature's palette: Exploring photosynthetic pigments for sustainable biotechnology	5
Enhanced purification of uterine smooth muscle cells from adenomyosis using a novel dual-enzyme digestion method	5
Complete genome sequence and transcriptome response to vitamin C supplementation of Novacetimonas hansenii SI1 - producer of highly-stretchable cellulose	5
A step closer to continuous buffer preparation from solids: Predicting powder compaction and how to prevent it	5
Probing the determinants of the transglycosylation/hydrolysis partition in a retaining α-l-arabinofuranosidase	5
A recombinant strain of Komagataeibacter xylinus ATCC 23770 for production of bacterial cellulose from mannose-rich resources	5
Natural deep eutectic solvents as thermostabilizer for Humicola insolens cutinase	4
Post-translational secretion stress regulation in Bacillus subtilis is controlled by intra- and extracellular proteases	4
CASPON platform technology: Ultrafast circularly permuted caspase-2 cleaves tagged fusion proteins before all 20 natural amino acids at the N-terminus	4
AI for life: Trends in artificial intelligence for biotechnology	4
Targeted mutagenesis in plants using Beet curly top virus for efficient delivery of CRISPR/Cas12a components	4
Impact of the pre-examination phase on multicenter metabolomic studies	4
Modifying flavor profiles of Saccharomyces spp. for industrial brewing using FIND-IT, a non-GMO approach for metabolic engineering of yeast	4
Machine translation of standardised medical terminology using natural language processing: A scoping review	4
Opinion: Independent third-party entities as a model for validation of commercial antibodies	4
Phytostabilization of metal(loid)s by ten emergent macrophytes following a 90-day exposure to industrially contaminated groundwater	4
Techno-economic assessment of bioethanol production from lignocellulose by consortium-based consolidated bioprocessing at industrial scale	4
Escherichia coli-based biorefining process yields optically pure lactic acid from fermented second-generation feedstocks	4