Nature Biomedical Engineering

Article	Citations
Author Correction: Optogenetic stimulation of vagal nerves for enhanced glucose-stimulated insulin secretion and β cell proliferation	586
Synaptic connectivity mapping among thousands of neurons via parallelized intracellular recording with a microhole electrode array	409
Emergency delivery of particulate drugs by active ejection using in vivo wireless devices	356
A humanized minipig model for the toxicological testing of therapeutic recombinant antibodies	345
Rapid and stain-free quantification of viral plaque via lens-free holography and deep learning	314
An implantable hydrogel-based phononic crystal for continuous and wireless monitoring of internal tissue strains	293
A nanoparticle probe for the imaging of autophagic flux in live mice via magnetic resonance and near-infrared fluorescence	272
Sensitive detection of SARS-CoV-2 on paper	260
Neuroimaging endophenotypes reveal underlying mechanisms and genetic factors contributing to progression and development of four brain disorders	259
A data-efficient strategy for building high-performing medical foundation models	258
Systemic immune responses to irradiated tumours via the transport of antigens to the tumour periphery by injected flagellate bacteria	249
Deep mutational learning for the selection of therapeutic antibodies resistant to the evolution of Omicron variants of SARS-CoV-2	225
A strain-programmed patch for the healing of diabetic wounds	221
Linking the genotypes and phenotypes of cancer cells in heterogenous populations via real-time optical tagging and image analysis	213
FAS-less allogeneic CAR T cells	208
Publisher Correction: Restoration of sensory feedback from the foot and reduction of phantom limb pain via closed-loop spinal cord stimulation	208
Author Correction: Endocisternal interfaces for minimally invasive neural stimulation and recording of the brain and spinal cord	207
A generative model uses healthy and diseased image pairs for pixel-level chest X-ray pathology localization	205
Biomaterial-induced conversion of quiescent cardiomyocytes into pacemaker cells in rats	204
Unbiased discovery of autoantibodies associated with severe COVID-19 via genome-scale self-assembled DNA-barcoded protein libraries	199
Intratumourally injected alum-tethered cytokines elicit potent and safer local and systemic anticancer immunity	197
Optogenetic stimulation of vagal nerves for enhanced glucose-stimulated insulin secretion and β cell proliferation	195
Large DNA deletions occur during DNA repair at 20-fold lower frequency for base editors and prime editors than for Cas9 nucleases	183
Ultrasensitive and long-lasting bioluminescence immunoassay for point-of-care viral antigen detection	181
Downregulating human leucocyte antigens on mesenchymal stromal cells by epigenetically repressing a β2-microglobulin super-enhancer	174

A sonosensitive diphenylalanine-based broad-spectrum antimicrobial peptide	173
Streamlining point-of-care assays	170
Cas9-mediated replacement of expanded CAG repeats in a pig model of Huntington’s disease	169
Modelling ciliopathy phenotypes in human tissues derived from pluripotent stem cells with genetically ablated cilia	169
Amelioration of post-traumatic osteoarthritis via nanoparticle depots delivering small interfering RNA to damaged cartilage	166
A high-density microfluidic bioreactor for the automated manufacturing of CAR T cells	163
Fast and sensitive electromechanical sensing	163
Designing receptor agonists with enhanced pharmacokinetics by grafting macrocyclic peptides into fragment crystallizable regions	162
Faster detection of somatic structural variants	161
Probing when dCas9 tolerates DNA mismatches	152
Potentiating cancer immunotherapies with modular albumin-hitchhiking nanobody–STING agonist conjugates	148
Simultaneous visualization of multiple radionuclides in vivo	143
Making fitter cells and tissues	140
A deep-learning model for quantifying circulating tumour DNA from the density distribution of DNA-fragment lengths	139
Offbeat drug-delivery tactics	136
Engineered allogeneic T cells decoupling T-cell-receptor and CD3 signalling enhance the antitumour activity of bispecific antibodies	132
Masking the immunotoxicity of interleukin-12 by fusing it with a domain of its receptor via a tumour-protease-cleavable linker	132
Generation of antigen-specific mature T cells from RAG1−/−RAG2−/−B2M−/− stem cells by engineering their microenvironment	132
Prevention and treatment of peri-implant fibrosis by functionally inhibiting skeletal cells expressing the leptin receptor	129
Immune-privileged tissues formed from immunologically cloaked mouse embryonic stem cells survive long term in allogeneic hosts	128
Augmented lipid-nanoparticle-mediated in vivo genome editing in the lungs and spleen by disrupting Cas9 activity in the liver	124
An organoid-based screen for epigenetic inhibitors that stimulate antigen presentation and potentiate T-cell-mediated cytotoxicity	123
A base editor for the long-term restoration of auditory function in mice with recessive profound deafness	123
Targeting inflammation with chimeric antigen receptor macrophages using a signal switch	117
A two-phase point-of-care diagnostic device for bladder cancer	116
A knockout run for CRISPRed cells	116
Sensing haemodynamics via wearables in sync	114
Subcutaneous pancreatic islet transplantation without immunosuppression	114
Mapping light distribution in tissue by using MRI-detectable photosensitive liposomes	113
Multiplex imaging of amyloid-β plaques dynamics in living brains with quinoline-malononitrile-based probes	111
A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples	108
Challenges and opportunities of acquiring cortical recordings for chronic adaptive deep brain stimulation	108
Nanobody-based bispecific antibody engagers targeting CTLA-4 or PD-L1 for cancer immunotherapy	105
An off-the-shelf otoacoustic-emission probe for hearing screening via a smartphone	102
1H magnetic resonance spectroscopic imaging of deuterated glucose and of neurotransmitter metabolism at 7 T in the human brain	102
Targeting vaccines to dendritic cells by mimicking the processing and presentation of antigens in xenotransplant rejection	99
Making MRI increasingly useful	98
A bilingual speech neuroprosthesis driven by cortical articulatory representations shared between languages	96
A paper-based assay for the colorimetric detection of SARS-CoV-2 variants at single-nucleotide resolution	96
Reprogramming of fibroblasts into expandable cardiovascular progenitor cells via small molecules in xeno-free conditions	95
Straightforward, inexpensive and sensitive	94
Publisher Correction: Efficient in vivo base editing via single adenoassociated viruses with size-optimized genomes encoding compact adenine base editors	91
Deep screening of antibody–antigen affinities	91
Mutation corrections in spinal muscular atrophy	91
Ablation of FAS confers allogeneic CD3– CAR T cells with resistance to rejection by T cells and natural killer cells	85
CAR T cells expressing a bacterial virulence factor trigger potent bystander antitumour responses in solid cancers	85
An injectable subcutaneous colon-specific immune niche for the treatment of ulcerative colitis	83
Opportunities and challenges in the development of exoskeletons for locomotor assistance	82
Screening for lipid nanoparticles that modulate the immune activity of helper T cells towards enhanced antitumour activity	81
Enhancement of the viability of T cells electroporated with DNA via osmotic dampening of the DNA-sensing cGAS–STING pathway	80

Synthetic cationic helical polypeptides for the stimulation of antitumour innate immune pathways in antigen-presenting cells	79
Targeted desialylation and cytolysis of tumour cells by fusing a sialidase to a bispecific T-cell engager	79
Author Correction: Restoration of tumour-growth suppression in vivo via systemic nanoparticle-mediated delivery of PTENmRNA	79
Profiling protein–protein interactions to predict the efficacy of B-cell-lymphoma-2-homology-3 mimetics for acute myeloid leukaemia	78
Eyeing severe diabetes upfront	78
Rapid adaptation of brain–computer interfaces to new neuronal ensembles or participants via generative modelling	78
Allometrically scaling tissue forces drive pathological foreign-body responses to implants via Rac2-activated myeloid cells	77
Mapping tissue heterogeneity in solid tumours using PET–MRI and machine learning	75
A serine-conjugated butyrate prodrug with high oral bioavailability suppresses autoimmune arthritis and neuroinflammation in mice	74
Accelerating primer design for amplicon sequencing using large language model-powered agents	72
A battery-less wireless implant for the continuous monitoring of vascular pressure, flow rate and temperature	71
Isolation of tumour-reactive lymphocytes from peripheral blood via microfluidic immunomagnetic cell sorting	71
Peptide-mediated delivery of CRISPR enzymes for the efficient editing of primary human lymphocytes	71
Insulin independence from stem-cell-derived islets	69
Explaining counterfactual images	69
Author Correction: Enhancing the immunogenicity of lipid-nanoparticle mRNA vaccines by adjuvanting the ionizable lipid and the mRNA	68
Author Correction: Thermally sentient bionic limbs	68
Mineralized bacteria as an immunotherapy agent against various cancer types	67
Engineering multi-specific nano-antibodies for cancer immunotherapy	66
Concurrently depleting regulatory T cells and activating cytotoxic T cells in tumours	66
Antibiotic-mediated selection of randomly mutagenized and cytokine-expressing oncolytic viruses	65
Addendum: A tissue-engineered scale model of the heart ventricle	65
Rational immunosilencing of a promiscuous T-cell epitope in the capsid of an adeno-associated virus	65
Synthetic libraries of immune cells displaying a diverse repertoire of chimaeric antigen receptors as a potent cancer immunotherapy	64
Generation of T-cell-receptor-negative CD8αβ-positive CAR T cells from T-cell-derived induced pluripotent stem cells	63
Low-cost and scalable projected light-sheet microscopy for the high-resolution imaging of cleared tissue and living samples	63
Spatial multi-omics at subcellular resolution via high-throughput in situ pairwise sequencing	61
Mining for encrypted peptide antibiotics in the human proteome	61
Osteopontin attenuates the foreign-body response to silicone implants	61
Enhanced intratumoural activity of CAR T cells engineered to produce immunomodulators under photothermal control	61
Opportunities and challenges in cardiac tissue engineering from an analysis of two decades of advances	61
Potent prophylactic cancer vaccines harnessing surface antigens shared by tumour cells and induced pluripotent stem cells	60
Induction and expansion of human PRRX1+ limb-bud-like mesenchymal cells from pluripotent stem cells	60
Multi-antigen spherical nucleic acid cancer vaccines	59
Base editing ablates all protein-coding genes of the mitochondrial genome in mouse cells	59
Write accessibly	59
Tooling up against mechanobiological orthodoxy	58
Efficient production of non-immunogenic, long circular RNAs for high protein yield	58
Publisher Correction: Targeting overexpressed antigens in glioblastoma via CAR T cells with computationally designed high-affinity protein binders	58
Precise virulence inactivation using a CRISPR-associated transposase for combating Enterobacteriaceae gut pathogens	57
Derivation of prognostic contextual histopathological features from whole-slide images of tumours via graph deep learning	57
A window into solid stresses within tumours	57
Meta-analysis of the make-up and properties of in vitro models of the healthy and diseased blood–brain barrier	57
Biomimetic model to study penile dysfunctions	57
Generation of neural organoids for spinal-cord regeneration via the direct reprogramming of human astrocytes	56
Neutrophils bearing adhesive polymer micropatches as a drug-free cancer immunotherapy	56
Antitumour vaccination via the targeted proteolysis of antigens isolated from tumour lysates	56
Efficient circular RNA synthesis for potent rolling circle translation	56
Field validation of the performance of paper-based tests for the detection of the Zika and chikungunya viruses in serum samples	56
Antigen-bearing outer membrane vesicles as tumour vaccines produced in situ by ingested genetically engineered bacteria	55
Single-cell analysis of ductal differentiation	55
How to make cover letters instructive	54
Towards handy neural prostheses	54
Author Correction: Targeted tumour theranostics in mice via carbon quantum dots structurally mimicking large amino acids	54
Seeing ageing through retinal images	53
Patterned gastrointestinal monolayers with bilateral access as observable models of parasite gut infection	53
Mapping and targeted viral activation of pancreatic nerves in mice reveal their roles in the regulation of glucose metabolism	53
Designing stable humanized antibodies	53
Identification of druggable regulators of cell secretion via a kinome-wide screen and high-throughput immunomagnetic cell sorting	52
Control of the antitumour activity and specificity of CAR T cells via organic adapters covalently tethering the CAR to tumour cells	51
Wirelessly operated bioelectronic sutures for the monitoring of deep surgical wounds	50
DNA-based computation for multiple biomarkers	50
Overcoming immune hurdles to implant longevity	50
Optimization of base editors for the functional correction of SMN2 as a treatment for spinal muscular atrophy	50
Nutritional deficiency in an intestine-on-a-chip recapitulates injury hallmarks associated with environmental enteric dysfunction	49
Lipid nanoparticles for the inhalation of mRNA	49
A low-power stretchable neuromorphic nerve with proprioceptive feedback	49
A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs	48
Author Correction: Mannich reaction-based combinatorial libraries identify antioxidant ionizable lipids for mRNA delivery with reduced immunogenicity	48
Aberrant chromatin reorganization in cells from diseased fibrous connective tissue in response to altered chemomechanical cues	48
Immunization against Zika by entrapping live virus in a subcutaneous self-adjuvanting hydrogel	47
Fluorescence lifetime of injected indocyanine green as a universal marker of solid tumours in patients	46
Rejuvenation of tumour-specific T cells through bispecific antibodies targeting PD-L1 on dendritic cells	46
Targeted knock-ins with pseudovirus for the stable expression of large transgenes	45
Streamlined detection of SARS-CoV-2 via Cas13	45
Contextual learning is nearly all you need	44
Endocisternal interfaces for minimally invasive neural stimulation and recording of the brain and spinal cord	43
Stimulating localizable sensations	43
Antimicrobial peptides boosted by ultrasound	43
Hormonally mediated mechanical remodelling of human haematopoietic stem cells enhances their bone-marrow engraftment	43

A thermogalvanic cell dressing for smart wound monitoring and accelerated healing	42
Assessing the toxicity of bispecific antibodies	42
Cas9-induced targeted integration of large DNA payloads in primary human T cells via homology-mediated end-joining DNA repair	42
Arrayed CRISPR libraries for the genome-wide activation, deletion and silencing of human protein-coding genes	42
Clinical validation of a wearable ultrasound sensor of blood pressure	41
Quantification of intratumoural heterogeneity in mice and patients via machine-learning models trained on PET–MRI data	41
Macrophage-augmented organoids recapitulate the complex pathophysiology of viral diseases and enable development of multitarget therapeutics	41
A deep generative model for deciphering cellular dynamics and in silico drug discovery in complex diseases	41
Localized butyrate restores gut homeostasis	40
A swallowable X-ray dosimeter for the real-time monitoring of radiotherapy	40
A fast all-optical 3D photoacoustic scanner for clinical vascular imaging	40
A swallowable X-ray dosimeter	40
Reinforcement of the intestinal mucosal barrier via mucus-penetrating PEGylated bacteria	39
Elimination of acquired resistance to PD-1 blockade via the concurrent depletion of tumour cells and immunosuppressive cells	39
Label-free intraoperative histology of bone tissue via deep-learning-assisted ultraviolet photoacoustic microscopy	39
Dynamical flexible inference of nonlinear latent factors and structures in neural population activity	38
Making CRISPR more deliverable	37
Intradermally delivered mRNA-encapsulating extracellular vesicles for collagen-replacement therapy	37
Dynamic monitoring of thousands of biochemical analytes using microsampling	37
Efficient recovery of potent tumour-infiltrating lymphocytes through quantitative immunomagnetic cell sorting	35
Genome-wide Cas9-mediated screening of essential non-coding regulatory elements via libraries of paired single-guide RNAs	35
Immunogenic amines on lipid nanoparticles	34
Screening oral drugs for their interactions with the intestinal transportome via porcine tissue explants and machine learning	34
Multiplexed discrimination of SARS-CoV-2 variants via plasmonic-enhanced fluorescence in a portable and automated device	34
Safer and efficient base editing and prime editing via ribonucleoproteins delivered through optimized lipid-nanoparticle formulations	33
The analgesic potential of heavy water	33
Rapid discovery of high-affinity antibodies via massively parallel sequencing, ribosome display and affinity screening	32
A stealthy neural recorder for the study of behaviour in primates	32
CRISPR-GPT for agentic automation of gene-editing experiments	32
A compact, wireless system for continuous monitoring of breast milk expressed during breastfeeding	32
Transthoracic ultrasound localization microscopy of myocardial vasculature in patients	31
Intraoperative imaging in pathology-assisted surgery	31
A wearable electrochemical biosensor for the monitoring of metabolites and nutrients	31
A wireless patch for the monitoring of C-reactive protein in sweat	30
Nitroglycerin-responsive gene switch for the on-demand production of therapeutic proteins	30
Squeezing more out from clinical imaging	30
Bridging scales in brain connectivity	30
Targeting NK2R reverses cardiometabolic dysfunction	30
Paired CRISPR screening libraries for studying the function of the non-coding genome at scale	30
Refresh cell culture	30
Expanded toolkits for RNA circularization	29
Sensing the DNA-mismatch tolerance of catalytically inactive Cas9 via barcoded DNA nanostructures in solid-state nanopores	29
Delivery of local anaesthetics by a self-assembled supramolecular system mimicking their interactions with a sodium channel	29
Gene correction for sickle cell disease hits its prime	29
Urinary bioorthogonal reporters for the monitoring of the efficacy of chemotherapy for lung cancer and of associated kidney injury	29
A generalist model for enhancing brain MRIs	29
Solvent-mediated analgesia via the suppression of water permeation through TRPV1 ion channels	28
Genetically engineered and enucleated human mesenchymal stromal cells for the targeted delivery of therapeutics to diseased tissue	28
Holographic transcranial ultrasound neuromodulation enhances stimulation efficacy by cooperatively recruiting distributed brain circuits	28
Conjugation of glucosylated polymer chains to checkpoint blockade antibodies augments their efficacy and specificity for glioblastoma	28
Application of prime editing to the correction of mutations and phenotypes in adult mice with liver and eye diseases	28
Advanced glycation end-products as mediators of the aberrant crosslinking of extracellular matrix in scarred liver tissue	28
Advancing breast cancer risk stratification using multimodal AI	27
Fast capturing of deep blood flow	27
Activatable near-infrared probes for the detection of specific populations of tumour-infiltrating leukocytes in vivo and in urine	27
Mapping light distribution in the brain via MRI	27
Regeneration of infarcted mouse hearts by cardiovascular tissue formed via the direct reprogramming of mouse fibroblasts	27
Nanoprobes for X-ray-mediated theranostics	26
Panoramic photoacoustic computed tomography with learning-based classification enhances breast lesion characterization	26
‘CRISPRing’ with therapeutic cells	26
Self-organization of sinusoidal vessels in pluripotent stem cell-derived human liver bud organoids	26
Author Correction: Organ-specific metastases obtained by culturing colorectal cancer cells on tissue-specific decellularized scaffolds	25
Histopathological biomarkers for predicting the tumour accumulation of nanomedicines	25
Restoration of sensory feedback from the foot and reduction of phantom limb pain via closed-loop spinal cord stimulation	25
Diagnosis of paediatric tuberculosis by optically detecting two virulence factors on extracellular vesicles in blood samples	25
A deep learning system for detecting silent brain infarction and predicting stroke risk	25
Targeting the PIEZO1 pathway boosts T cell antitumour cytotoxicity	24
Suppression of cytokine release syndrome during CAR-T-cell therapy via a subcutaneously injected interleukin-6-adsorbing hydrogel	24
Closed-loop enhancement and neural decoding of cognitive control in humans	24
Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases and prime editing	24
Generation of functionally distinct T-cell populations by altering the viscoelasticity of their extracellular matrix	24
AAV-mediated delivery of a Sleeping Beauty transposon and an mRNA-encoded transposase for the engineering of therapeutic immune cells	23
A multi-organ chip with matured tissue niches linked by vascular flow	23
Tackling prediction uncertainty in machine learning for healthcare	23
Optimization of therapeutic antibodies for reduced self-association and non-specific binding via interpretable machine learning	23
Oncolytic mineralized bacteria as potent locally administered immunotherapeutics	23
Spatially resolved in vivo imaging of inflammation-associated mRNA via enzymatic fluorescence amplification in a molecular beacon	23
High-throughput spatiotemporal monitoring of single-cell secretions via plasmonic microwell arrays	22
Setting morphogen gradients	22
An instrument-free, programmable approach for nucleic acid detection	22
Quantitative imaging of apoptosis following oncolytic virotherapy by magnetic resonance fingerprinting aided by deep learning	22
Author Correction: An organoid-based screen for epigenetic inhibitors that stimulate antigen presentation and potentiate T-cell-mediated cytotoxicity	22
Fast volumetric scanning of living tissue	21
Pathological mechanosensing	21
Publisher Correction: Compression and amplification algorithms in hearing aids impair the selectivity of neural responses to speech	21
Textile-embedded cell-free biosensors	21
Oxonium ion scanning mass spectrometry for large-scale plasma glycoproteomics	20
Prevention of vascular-allograft rejection by protecting the endothelial glycocalyx with immunosuppressive polymers	20
Author Correction: Enhanced control of a brain–computer interface by tetraplegic participants via neural-network-mediated feature extraction	20
Author Correction: A low-power stretchable neuromorphic nerve with proprioceptive feedback	20
The myriad ways to engineer cells	20
A lentiviral vector for the production of T cells with an inducible transgene and a constitutively expressed tumour-targeting receptor	20
Surgical polarimetric endoscopy for the detection of laryngeal cancer	19
Deep brain stimulation by blood–brain-barrier-crossing piezoelectric nanoparticles generating current and nitric oxide under focused ultrasound	19
Detection of signs of disease in external photographs of the eyes via deep learning	19