Nature Biomedical Engineering

Article	Citations
Sensitive detection of SARS-CoV-2 on paper	792
A humanized minipig model for the toxicological testing of therapeutic recombinant antibodies	554
Author Correction: Optogenetic stimulation of vagal nerves for enhanced glucose-stimulated insulin secretion and β cell proliferation	513
Single-nucleus transcriptomics of an engineered pig model reveals microglia–T cell interactions driving Huntington’s disease neurodegeneration	373
Emergency delivery of particulate drugs by active ejection using in vivo wireless devices	359
Perilesional neuromodulation replaces lost sensorimotor function in persons with spinal cord injury	345
Rapid and stain-free quantification of viral plaque via lens-free holography and deep learning	316
Deep mutational learning for the selection of therapeutic antibodies resistant to the evolution of Omicron variants of SARS-CoV-2	308
Synaptic connectivity mapping among thousands of neurons via parallelized intracellular recording with a microhole electrode array	300
An implantable hydrogel-based phononic crystal for continuous and wireless monitoring of internal tissue strains	283
Neuroimaging endophenotypes reveal underlying mechanisms and genetic factors contributing to progression and development of four brain disorders	268
Brain–heart–eye axis revealed by multi-organ imaging genetics and proteomics	246
A data-efficient strategy for building high-performing medical foundation models	245
A nanoparticle probe for the imaging of autophagic flux in live mice via magnetic resonance and near-infrared fluorescence	235
Human gastric multi-regional assembloids for functional parietal maturation and patient-specific modelling of antral foveolar hyperplasia	232
A strain-programmed patch for the healing of diabetic wounds	227
A generative model uses healthy and diseased image pairs for pixel-level chest X-ray pathology localization	218
FAS-less allogeneic CAR T cells	213
Streamlining point-of-care assays	201
Three-dimensional architecture of human bone marrow	198
Ultrasensitive and long-lasting bioluminescence immunoassay for point-of-care viral antigen detection	181
Optogenetic stimulation of vagal nerves for enhanced glucose-stimulated insulin secretion and β cell proliferation	176
Autonomous ultrasound with UltraBot	170
Downregulating human leucocyte antigens on mesenchymal stromal cells by epigenetically repressing a β2-microglobulin super-enhancer	166
Author Correction: Endocisternal interfaces for minimally invasive neural stimulation and recording of the brain and spinal cord	156

Low-input deep learning platform for citrullinated peptide identification, autoantigen discovery and rheumatoid arthritis treatment stratification	155
Publisher Correction: Restoration of sensory feedback from the foot and reduction of phantom limb pain via closed-loop spinal cord stimulation	155
A sonosensitive diphenylalanine-based broad-spectrum antimicrobial peptide	151
Large DNA deletions occur during DNA repair at 20-fold lower frequency for base editors and prime editors than for Cas9 nucleases	151
A high-density microfluidic bioreactor for the automated manufacturing of CAR T cells	148
Systematic functional screening of switchable aptamer beacon probes	148
Unbiased discovery of autoantibodies associated with severe COVID-19 via genome-scale self-assembled DNA-barcoded protein libraries	142
Cas9-mediated replacement of expanded CAG repeats in a pig model of Huntington’s disease	141
Faster detection of somatic structural variants	135
Towards generalizable AI in medicine via Generalist–Specialist Collaboration	133
Generation of antigen-specific mature T cells from RAG1−/−RAG2−/−B2M−/− stem cells by engineering their microenvironment	133
Prevention and treatment of peri-implant fibrosis by functionally inhibiting skeletal cells expressing the leptin receptor	131
A deep-learning model for quantifying circulating tumour DNA from the density distribution of DNA-fragment lengths	129
A warm introduction	127
Offbeat drug-delivery tactics	124
Engineered non-expansive matrix for blood vessel organoid development and ischaemic stroke repair	122
Designing receptor agonists with enhanced pharmacokinetics by grafting macrocyclic peptides into fragment crystallizable regions	120
Fluid shear stress activates a targetable mechano-metastatic cascade to promote medulloblastoma metastasis	119
Anchored random reverse primer sequencing for quantitative detection of novel gene fusions	118
Probing when dCas9 tolerates DNA mismatches	116
Engineered allogeneic T cells decoupling T-cell-receptor and CD3 signalling enhance the antitumour activity of bispecific antibodies	110
Publisher Correction: Large-scale generation of iNK and CAR-iNK cells from CD34+ haematopoietic stem and progenitor cells for adoptive immunotherapy	109
LDL-binding IL-10 reduces vascular inflammation in atherosclerotic mice	108
A filamentary soft robotic probe for multimodal in utero monitoring of fetal health	107
Immune-privileged tissues formed from immunologically cloaked mouse embryonic stem cells survive long term in allogeneic hosts	104
A base editor for the long-term restoration of auditory function in mice with recessive profound deafness	102
Potentiating cancer immunotherapies with modular albumin-hitchhiking nanobody–STING agonist conjugates	100
Engineering challenges and translational opportunities in emerging gene delivery platforms	96
Targeting inflammation with chimeric antigen receptor macrophages using a signal switch	95
A two-phase point-of-care diagnostic device for bladder cancer	94
A knockout run for CRISPRed cells	94
Sensing haemodynamics via wearables in sync	90
A deep learning and large language hybrid workflow for omics interpretation	88
Subcutaneous pancreatic islet transplantation without immunosuppression	88
X-ray activated platinum complex induces DNA damage and enhances cancer immunotherapy through abscopal effect	86
mRNA delivery to the endometrium	84
Making MRI increasingly useful	84
A bilingual speech neuroprosthesis driven by cortical articulatory representations shared between languages	83
A three-dimensional multi-modal foundation model for optical coherence tomography	83
Targeting vaccines to dendritic cells by mimicking the processing and presentation of antigens in xenotransplant rejection	82
Multiplex imaging of amyloid-β plaques dynamics in living brains with quinoline-malononitrile-based probes	81
A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples	80
Shape-conformal porous frameworks for full coverage of neural organoids and high-resolution electrophysiology	80
Mapping light distribution in tissue by using MRI-detectable photosensitive liposomes	80
A paper-based assay for the colorimetric detection of SARS-CoV-2 variants at single-nucleotide resolution	80
Nanobody-based bispecific antibody engagers targeting CTLA-4 or PD-L1 for cancer immunotherapy	80
1H magnetic resonance spectroscopic imaging of deuterated glucose and of neurotransmitter metabolism at 7 T in the human brain	78
Mapping tissue heterogeneity in solid tumours using PET–MRI and machine learning	78
Author Correction: Implanted microelectrode arrays in reinnervated muscles allow separation of neural drives from transferred polyfunctional nerves	78
An off-the-shelf otoacoustic-emission probe for hearing screening via a smartphone	78

Challenges and opportunities of acquiring cortical recordings for chronic adaptive deep brain stimulation	78
Mutation corrections in spinal muscular atrophy	77
Deep screening of antibody–antigen affinities	77
Publisher Correction: Efficient in vivo base editing via single adenoassociated viruses with size-optimized genomes encoding compact adenine base editors	76
Where the nanocarriers go	72
In vivo base editing rescues liver pathophysiology and peroxisome dysfunction in a mouse model of Zellweger spectrum disorder	71
Straightforward, inexpensive and sensitive	70
Accelerating primer design for amplicon sequencing using large language model-powered agents	70
An injectable subcutaneous colon-specific immune niche for the treatment of ulcerative colitis	69
Screening for lipid nanoparticles that modulate the immune activity of helper T cells towards enhanced antitumour activity	69
Empowering AI data scientists using a multi-agent LLM framework with self-evolving capabilities for autonomous, tool-aware biomedical data analyses	68
Enhancement of the viability of T cells electroporated with DNA via osmotic dampening of the DNA-sensing cGAS–STING pathway	68
Isolation of tumour-reactive lymphocytes from peripheral blood via microfluidic immunomagnetic cell sorting	67
Label-free tissue NIR-II autofluorescence imaging for visualization of human liver malignancy	67
Profiling protein–protein interactions to predict the efficacy of B-cell-lymphoma-2-homology-3 mimetics for acute myeloid leukaemia	67
Author Correction: Restoration of tumour-growth suppression in vivo via systemic nanoparticle-mediated delivery of PTENmRNA	67
Ablation of FAS confers allogeneic CD3– CAR T cells with resistance to rejection by T cells and natural killer cells	66
Peptide-mediated delivery of CRISPR enzymes for the efficient editing of primary human lymphocytes	64
Symbiotic transcatheter pacemaker for lifelong energy regeneration and therapeutic function in porcine disease model	64
Allometrically scaling tissue forces drive pathological foreign-body responses to implants via Rac2-activated myeloid cells	63
Engineered thoracic spinal cord organoids for transplantation after spinal cord injury	63
A battery-less wireless implant for the continuous monitoring of vascular pressure, flow rate and temperature	62
A serine-conjugated butyrate prodrug with high oral bioavailability suppresses autoimmune arthritis and neuroinflammation in mice	61
Synthetic cationic helical polypeptides for the stimulation of antitumour innate immune pathways in antigen-presenting cells	60
Targeted desialylation and cytolysis of tumour cells by fusing a sialidase to a bispecific T-cell engager	60
Opportunities and challenges in the development of exoskeletons for locomotor assistance	59
Insulin independence from stem-cell-derived islets	59
Targeting myeloid cell-mediated fibrosis through FAK	58
Concurrently depleting regulatory T cells and activating cytotoxic T cells in tumours	58
Mineralized bacteria as an immunotherapy agent against various cancer types	58
Explaining counterfactual images	58
Author Correction: Thermally sentient bionic limbs	58
The ever-changing communication of scientific discovery	57
On the horizon in biomedical engineering	57
Osteopontin attenuates the foreign-body response to silicone implants	57
Rational immunosilencing of a promiscuous T-cell epitope in the capsid of an adeno-associated virus	56
Antibiotic-mediated selection of randomly mutagenized and cytokine-expressing oncolytic viruses	56
A geometry aware framework enhances noninvasive mapping of whole human brain dynamics	56
Low-cost and scalable projected light-sheet microscopy for the high-resolution imaging of cleared tissue and living samples	56
Directed evolution-based discovery of ligands for in vivo restimulation of chimeric antigen receptor T cells	54
Engineering multi-specific nano-antibodies for cancer immunotherapy	54
Fundamental limitations of kilohertz-frequency carriers in afferent fibre recruitment with transcutaneous spinal cord stimulation	54
Author Correction: Enhancing the immunogenicity of lipid-nanoparticle mRNA vaccines by adjuvanting the ionizable lipid and the mRNA	53
Synthetic libraries of immune cells displaying a diverse repertoire of chimaeric antigen receptors as a potent cancer immunotherapy	53
Multi-antigen spherical nucleic acid cancer vaccines	52
Automated disc device for multiplexed extracellular vesicle isolation and labelling from liquid biopsies in cancer diagnostics	51
Potent prophylactic cancer vaccines harnessing surface antigens shared by tumour cells and induced pluripotent stem cells	50
Spatial multi-omics at subcellular resolution via high-throughput in situ pairwise sequencing	50
Translating black-box medical AI models into interpretable global decision logic	50
Generation of T-cell-receptor-negative CD8αβ-positive CAR T cells from T-cell-derived induced pluripotent stem cells	50
Learning neuroimaging models from health system-scale data	49
Tooling up against mechanobiological orthodoxy	49
Base editing ablates all protein-coding genes of the mitochondrial genome in mouse cells	49
A window into solid stresses within tumours	49
Generation of neural organoids for spinal-cord regeneration via the direct reprogramming of human astrocytes	48
Neutrophils bearing adhesive polymer micropatches as a drug-free cancer immunotherapy	48
Tumour-specific bioorthogonal synthesis of proteolysis-targeting chimeras and nanoparticles boosts T cell activity	47
Precise virulence inactivation using a CRISPR-associated transposase for combating Enterobacteriaceae gut pathogens	47
Biomimetic model to study penile dysfunctions	46
Meta-analysis of the make-up and properties of in vitro models of the healthy and diseased blood–brain barrier	46
Efficient production of non-immunogenic, long circular RNAs for high protein yield	46
Derivation of prognostic contextual histopathological features from whole-slide images of tumours via graph deep learning	45
Label-free navigation system for grading prostate tumour malignancy in situ via tissue pH and prostate-specific antigen activity	45
Antitumour vaccination via the targeted proteolysis of antigens isolated from tumour lysates	45
Efficient circular RNA synthesis for potent rolling circle translation	44
One-day rapid sterility test for human-derived biopharmaceuticals	44
Publisher Correction: Targeting overexpressed antigens in glioblastoma via CAR T cells with computationally designed high-affinity protein binders	44
DNA-based computation for multiple biomarkers	43
Towards handy neural prostheses	43
How to make cover letters instructive	43
Theoretical quantitative model and clinical outcome predictions of conductive cardiac patches for electrophysiological treatments	43
Seeing ageing through retinal images	43
Identification of druggable regulators of cell secretion via a kinome-wide screen and high-throughput immunomagnetic cell sorting	42
Whole cross-sectional human ultrasound tomography	42
Designing stable humanized antibodies	42
Publisher Correction: Non-invasive characterization of pericyte dysfunction in mouse brain using functional ultrasound localization microscopy	42
Overcoming immune hurdles to implant longevity	42
Author Correction: Mannich reaction-based combinatorial libraries identify antioxidant ionizable lipids for mRNA delivery with reduced immunogenicity	41
Immunization against Zika by entrapping live virus in a subcutaneous self-adjuvanting hydrogel	41
A generalist foundation model and database for open-world medical image segmentation	41

Mapping and targeted viral activation of pancreatic nerves in mice reveal their roles in the regulation of glucose metabolism	41
Patterned gastrointestinal monolayers with bilateral access as observable models of parasite gut infection	41
Control of the antitumour activity and specificity of CAR T cells via organic adapters covalently tethering the CAR to tumour cells	41
Optimization of base editors for the functional correction of SMN2 as a treatment for spinal muscular atrophy	41
Fluorescence lifetime of injected indocyanine green as a universal marker of solid tumours in patients	40
A low-power stretchable neuromorphic nerve with proprioceptive feedback	39
Beyond moonshots	39
Continuous insulin monitoring using an antibody-protecting zwitterionic microneedle patch	39
Nutritional deficiency in an intestine-on-a-chip recapitulates injury hallmarks associated with environmental enteric dysfunction	38
Targeted knock-ins with pseudovirus for the stable expression of large transgenes	38
Aberrant chromatin reorganization in cells from diseased fibrous connective tissue in response to altered chemomechanical cues	38
Artificial intelligence advances skull stripping across lifespan	38
A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs	38
Stimulating localizable sensations	38
Antimicrobial peptides boosted by ultrasound	37
Localized butyrate restores gut homeostasis	37
A swallowable X-ray dosimeter	37
Quantification of intratumoural heterogeneity in mice and patients via machine-learning models trained on PET–MRI data	37
Hormonally mediated mechanical remodelling of human haematopoietic stem cells enhances their bone-marrow engraftment	37
A deep generative model for deciphering cellular dynamics and in silico drug discovery in complex diseases	37
Contextual learning is nearly all you need	37
Assessing the toxicity of bispecific antibodies	37
Dynamical flexible inference of nonlinear latent factors and structures in neural population activity	36
Cas9-induced targeted integration of large DNA payloads in primary human T cells via homology-mediated end-joining DNA repair	36
Streamlined detection of SARS-CoV-2 via Cas13	36
Endocisternal interfaces for minimally invasive neural stimulation and recording of the brain and spinal cord	36
Team updates at Nature Biomedical Engineering	35
Arrayed CRISPR libraries for the genome-wide activation, deletion and silencing of human protein-coding genes	35
Label-free intraoperative histology of bone tissue via deep-learning-assisted ultraviolet photoacoustic microscopy	35
A swallowable X-ray dosimeter for the real-time monitoring of radiotherapy	35
Thermoreversibly assembled polymersomes for highly efficient loading, processing and delivery of protein and siRNA biologics	35
Macrophage-augmented organoids recapitulate the complex pathophysiology of viral diseases and enable development of multitarget therapeutics	35
A fast all-optical 3D photoacoustic scanner for clinical vascular imaging	34
4D-printed adaptive hydrogel tissue expanders for ear and breast reconstruction	34
Clinical validation of a wearable ultrasound sensor of blood pressure	34
Treatment of a severe vascular disease using a bespoke CRISPR–Cas9 base editor in mice	34
Reinforcement of the intestinal mucosal barrier via mucus-penetrating PEGylated bacteria	33
Minimally invasive implantation of scalable high-density cortical microelectrode arrays for multimodal neural decoding and stimulation	33
A thermogalvanic cell dressing for smart wound monitoring and accelerated healing	33
Intradermally delivered mRNA-encapsulating extracellular vesicles for collagen-replacement therapy	33
Dynamic monitoring of thousands of biochemical analytes using microsampling	32
Immunogenic amines on lipid nanoparticles	32
Confounding factors and biases abound when predicting molecular biomarkers from histological images	31
The analgesic potential of heavy water	31
A foundation generative model for breast ultrasound image analysis	31
Making CRISPR more deliverable	31
Genome-wide Cas9-mediated screening of essential non-coding regulatory elements via libraries of paired single-guide RNAs	31
Accelerated sterility testing unlocks safe delivery of life-saving therapies	30
Screening oral drugs for their interactions with the intestinal transportome via porcine tissue explants and machine learning	30
A vision–language pretrained transformer for versatile clinical respiratory disease applications	30
CRISPR-GPT for agentic automation of gene-editing experiments	29
A stealthy neural recorder for the study of behaviour in primates	29
A compact, wireless system for continuous monitoring of breast milk expressed during breastfeeding	29
Predictive biomarkers of response to chimeric antigen receptor (CAR) T-cell therapy for pan-haematologic cancer	28
Rapid discovery of high-affinity antibodies via massively parallel sequencing, ribosome display and affinity screening	28
Safer and efficient base editing and prime editing via ribonucleoproteins delivered through optimized lipid-nanoparticle formulations	28
Transthoracic ultrasound localization microscopy of myocardial vasculature in patients	28
A wearable electrochemical biosensor for the monitoring of metabolites and nutrients	27
Multiplexed discrimination of SARS-CoV-2 variants via plasmonic-enhanced fluorescence in a portable and automated device	27
Unconditional latent diffusion models memorize patient imaging data	27
Squeezing more out from clinical imaging	27
Benchmarking foundation models as feature extractors for weakly supervised computational pathology	27
A wireless patch for the monitoring of C-reactive protein in sweat	27
Building foundation models for cardiac MRI	26
Targeting NK2R reverses cardiometabolic dysfunction	26
Paired CRISPR screening libraries for studying the function of the non-coding genome at scale	26
Wireless and bioresorbable triboelectric nerve block system for postoperative pain control	26
Bridging scales in brain connectivity	26
CCR5-targeted allogeneic gamma–delta CD19 chimeric antigen receptor T cells for HIV-associated B cell-malignancy immunotherapy	26
A generalist model for enhancing brain MRIs	26
Activatable near-infrared probes for the detection of specific populations of tumour-infiltrating leukocytes in vivo and in urine	25
An artificial cilia-based array system for sound frequency decoding and resonance-responsive drug release	25
Expanded toolkits for RNA circularization	25
Sensing the DNA-mismatch tolerance of catalytically inactive Cas9 via barcoded DNA nanostructures in solid-state nanopores	25
Solvent-mediated analgesia via the suppression of water permeation through TRPV1 ion channels	25
Urinary bioorthogonal reporters for the monitoring of the efficacy of chemotherapy for lung cancer and of associated kidney injury	25
Gene correction for sickle cell disease hits its prime	25
A HO-1 gene knockout using a NanoCRISPR scaffold suppresses metastasis in mouse models	25
Holographic transcranial ultrasound neuromodulation enhances stimulation efficacy by cooperatively recruiting distributed brain circuits	25
Generalist foundation models from a multimodal dataset for 3D computed tomography	25
Boosting prime editing with engineered non-canonical pegRNAs	25
Nitroglycerin-responsive gene switch for the on-demand production of therapeutic proteins	25
Advanced glycation end-products as mediators of the aberrant crosslinking of extracellular matrix in scarred liver tissue	24
‘CRISPRing’ with therapeutic cells	24
Targeting the PIEZO1 pathway boosts T cell antitumour cytotoxicity	24
Mapping light distribution in the brain via MRI	24
Suppression of cytokine release syndrome during CAR-T-cell therapy via a subcutaneously injected interleukin-6-adsorbing hydrogel	24
Advancing breast cancer risk stratification using multimodal AI	24
Nanoprobes for X-ray-mediated theranostics	24
Fast capturing of deep blood flow	24
Author Correction: Organ-specific metastases obtained by culturing colorectal cancer cells on tissue-specific decellularized scaffolds	24
Oncolytic mineralized bacteria as potent locally administered immunotherapeutics	23
Spatially resolved in vivo imaging of inflammation-associated mRNA via enzymatic fluorescence amplification in a molecular beacon	23
AAV-mediated delivery of a Sleeping Beauty transposon and an mRNA-encoded transposase for the engineering of therapeutic immune cells	23
Author Correction: Extracellular vesicles incorporating retrovirus-like capsids for the enhanced packaging and systemic delivery of mRNA into neurons	22