Nature Biomedical Engineering

Article	Citations
Author Correction: Optogenetic stimulation of vagal nerves for enhanced glucose-stimulated insulin secretion and β cell proliferation	654
Deep mutational learning for the selection of therapeutic antibodies resistant to the evolution of Omicron variants of SARS-CoV-2	464
Neuroimaging endophenotypes reveal underlying mechanisms and genetic factors contributing to progression and development of four brain disorders	416
Brain–heart–eye axis revealed by multi-organ imaging genetics and proteomics	350
A humanized minipig model for the toxicological testing of therapeutic recombinant antibodies	346
An implantable hydrogel-based phononic crystal for continuous and wireless monitoring of internal tissue strains	292
Synaptic connectivity mapping among thousands of neurons via parallelized intracellular recording with a microhole electrode array	288
Emergency delivery of particulate drugs by active ejection using in vivo wireless devices	279
A nanoparticle probe for the imaging of autophagic flux in live mice via magnetic resonance and near-infrared fluorescence	261
Sensitive detection of SARS-CoV-2 on paper	258
A data-efficient strategy for building high-performing medical foundation models	249
Rapid and stain-free quantification of viral plaque via lens-free holography and deep learning	248
Systemic immune responses to irradiated tumours via the transport of antigens to the tumour periphery by injected flagellate bacteria	244
A strain-programmed patch for the healing of diabetic wounds	238
A generative model uses healthy and diseased image pairs for pixel-level chest X-ray pathology localization	231
Author Correction: Endocisternal interfaces for minimally invasive neural stimulation and recording of the brain and spinal cord	224
FAS-less allogeneic CAR T cells	215
Downregulating human leucocyte antigens on mesenchymal stromal cells by epigenetically repressing a β2-microglobulin super-enhancer	214
Publisher Correction: Restoration of sensory feedback from the foot and reduction of phantom limb pain via closed-loop spinal cord stimulation	212
Streamlining point-of-care assays	212
Systematic functional screening of switchable aptamer beacon probes	207
Unbiased discovery of autoantibodies associated with severe COVID-19 via genome-scale self-assembled DNA-barcoded protein libraries	197
Optogenetic stimulation of vagal nerves for enhanced glucose-stimulated insulin secretion and β cell proliferation	193
Autonomous ultrasound with UltraBot	193
A sonosensitive diphenylalanine-based broad-spectrum antimicrobial peptide	190

Biomaterial-induced conversion of quiescent cardiomyocytes into pacemaker cells in rats	188
Modelling ciliopathy phenotypes in human tissues derived from pluripotent stem cells with genetically ablated cilia	183
Cas9-mediated replacement of expanded CAG repeats in a pig model of Huntington’s disease	182
A high-density microfluidic bioreactor for the automated manufacturing of CAR T cells	180
Intratumourally injected alum-tethered cytokines elicit potent and safer local and systemic anticancer immunity	170
Linking the genotypes and phenotypes of cancer cells in heterogenous populations via real-time optical tagging and image analysis	163
Ultrasensitive and long-lasting bioluminescence immunoassay for point-of-care viral antigen detection	161
Large DNA deletions occur during DNA repair at 20-fold lower frequency for base editors and prime editors than for Cas9 nucleases	157
Faster detection of somatic structural variants	155
Probing when dCas9 tolerates DNA mismatches	146
Fluid shear stress activates a targetable mechano-metastatic cascade to promote medulloblastoma metastasis	146
A warm introduction	144
Offbeat drug-delivery tactics	142
Fast and sensitive electromechanical sensing	141
Generation of antigen-specific mature T cells from RAG1−/−RAG2−/−B2M−/− stem cells by engineering their microenvironment	141
A deep-learning model for quantifying circulating tumour DNA from the density distribution of DNA-fragment lengths	137
Making fitter cells and tissues	135
Designing receptor agonists with enhanced pharmacokinetics by grafting macrocyclic peptides into fragment crystallizable regions	132
A base editor for the long-term restoration of auditory function in mice with recessive profound deafness	131
Augmented lipid-nanoparticle-mediated in vivo genome editing in the lungs and spleen by disrupting Cas9 activity in the liver	127
Potentiating cancer immunotherapies with modular albumin-hitchhiking nanobody–STING agonist conjugates	124
Prevention and treatment of peri-implant fibrosis by functionally inhibiting skeletal cells expressing the leptin receptor	122
Immune-privileged tissues formed from immunologically cloaked mouse embryonic stem cells survive long term in allogeneic hosts	122
An organoid-based screen for epigenetic inhibitors that stimulate antigen presentation and potentiate T-cell-mediated cytotoxicity	115
Targeting inflammation with chimeric antigen receptor macrophages using a signal switch	113
Masking the immunotoxicity of interleukin-12 by fusing it with a domain of its receptor via a tumour-protease-cleavable linker	110
Simultaneous visualization of multiple radionuclides in vivo	110
A knockout run for CRISPRed cells	109
Engineered allogeneic T cells decoupling T-cell-receptor and CD3 signalling enhance the antitumour activity of bispecific antibodies	109
A two-phase point-of-care diagnostic device for bladder cancer	107
Sensing haemodynamics via wearables in sync	105
Subcutaneous pancreatic islet transplantation without immunosuppression	104
Making MRI increasingly useful	103
Mapping light distribution in tissue by using MRI-detectable photosensitive liposomes	99
An off-the-shelf otoacoustic-emission probe for hearing screening via a smartphone	98
A bilingual speech neuroprosthesis driven by cortical articulatory representations shared between languages	96
1H magnetic resonance spectroscopic imaging of deuterated glucose and of neurotransmitter metabolism at 7 T in the human brain	94
Nanobody-based bispecific antibody engagers targeting CTLA-4 or PD-L1 for cancer immunotherapy	93
Multiplex imaging of amyloid-β plaques dynamics in living brains with quinoline-malononitrile-based probes	92
A paper-based assay for the colorimetric detection of SARS-CoV-2 variants at single-nucleotide resolution	89
Challenges and opportunities of acquiring cortical recordings for chronic adaptive deep brain stimulation	89
A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples	89
Targeting vaccines to dendritic cells by mimicking the processing and presentation of antigens in xenotransplant rejection	88
Reprogramming of fibroblasts into expandable cardiovascular progenitor cells via small molecules in xeno-free conditions	88
Mapping tissue heterogeneity in solid tumours using PET–MRI and machine learning	87
Deep screening of antibody–antigen affinities	86
Mutation corrections in spinal muscular atrophy	86
Publisher Correction: Efficient in vivo base editing via single adenoassociated viruses with size-optimized genomes encoding compact adenine base editors	85
Author Correction: Restoration of tumour-growth suppression in vivo via systemic nanoparticle-mediated delivery of PTENmRNA	84
Isolation of tumour-reactive lymphocytes from peripheral blood via microfluidic immunomagnetic cell sorting	83

Synthetic cationic helical polypeptides for the stimulation of antitumour innate immune pathways in antigen-presenting cells	83
Profiling protein–protein interactions to predict the efficacy of B-cell-lymphoma-2-homology-3 mimetics for acute myeloid leukaemia	81
Targeted desialylation and cytolysis of tumour cells by fusing a sialidase to a bispecific T-cell engager	78
A battery-less wireless implant for the continuous monitoring of vascular pressure, flow rate and temperature	77
Opportunities and challenges in the development of exoskeletons for locomotor assistance	76
CAR T cells expressing a bacterial virulence factor trigger potent bystander antitumour responses in solid cancers	75
Enhancement of the viability of T cells electroporated with DNA via osmotic dampening of the DNA-sensing cGAS–STING pathway	75
Allometrically scaling tissue forces drive pathological foreign-body responses to implants via Rac2-activated myeloid cells	74
A serine-conjugated butyrate prodrug with high oral bioavailability suppresses autoimmune arthritis and neuroinflammation in mice	73
Peptide-mediated delivery of CRISPR enzymes for the efficient editing of primary human lymphocytes	73
Ablation of FAS confers allogeneic CD3– CAR T cells with resistance to rejection by T cells and natural killer cells	73
Straightforward, inexpensive and sensitive	73
Eyeing severe diabetes upfront	72
An injectable subcutaneous colon-specific immune niche for the treatment of ulcerative colitis	71
Accelerating primer design for amplicon sequencing using large language model-powered agents	70
Rapid adaptation of brain–computer interfaces to new neuronal ensembles or participants via generative modelling	69
Engineered thoracic spinal cord organoids for transplantation after spinal cord injury	69
Screening for lipid nanoparticles that modulate the immune activity of helper T cells towards enhanced antitumour activity	69
Author Correction: Thermally sentient bionic limbs	68
Insulin independence from stem-cell-derived islets	68
Mineralized bacteria as an immunotherapy agent against various cancer types	67
Concurrently depleting regulatory T cells and activating cytotoxic T cells in tumours	67
Explaining counterfactual images	67
Engineering multi-specific nano-antibodies for cancer immunotherapy	66
Rational immunosilencing of a promiscuous T-cell epitope in the capsid of an adeno-associated virus	66
The ever-changing communication of scientific discovery	66
Synthetic libraries of immune cells displaying a diverse repertoire of chimaeric antigen receptors as a potent cancer immunotherapy	66
Generation of T-cell-receptor-negative CD8αβ-positive CAR T cells from T-cell-derived induced pluripotent stem cells	65
Spatial multi-omics at subcellular resolution via high-throughput in situ pairwise sequencing	64
Potent prophylactic cancer vaccines harnessing surface antigens shared by tumour cells and induced pluripotent stem cells	63
Antibiotic-mediated selection of randomly mutagenized and cytokine-expressing oncolytic viruses	63
Directed evolution-based discovery of ligands for in vivo restimulation of chimeric antigen receptor T cells	63
Opportunities and challenges in cardiac tissue engineering from an analysis of two decades of advances	62
Osteopontin attenuates the foreign-body response to silicone implants	62
Targeting myeloid cell-mediated fibrosis through FAK	62
Multi-antigen spherical nucleic acid cancer vaccines	62
Author Correction: Enhancing the immunogenicity of lipid-nanoparticle mRNA vaccines by adjuvanting the ionizable lipid and the mRNA	62
Low-cost and scalable projected light-sheet microscopy for the high-resolution imaging of cleared tissue and living samples	62
Mining for encrypted peptide antibiotics in the human proteome	61
Addendum: A tissue-engineered scale model of the heart ventricle	61
Base editing ablates all protein-coding genes of the mitochondrial genome in mouse cells	60
Efficient circular RNA synthesis for potent rolling circle translation	59
Tooling up against mechanobiological orthodoxy	59
A window into solid stresses within tumours	59
Publisher Correction: Targeting overexpressed antigens in glioblastoma via CAR T cells with computationally designed high-affinity protein binders	59
Efficient production of non-immunogenic, long circular RNAs for high protein yield	58
Precise virulence inactivation using a CRISPR-associated transposase for combating Enterobacteriaceae gut pathogens	57
Derivation of prognostic contextual histopathological features from whole-slide images of tumours via graph deep learning	57
Biomimetic model to study penile dysfunctions	57
Meta-analysis of the make-up and properties of in vitro models of the healthy and diseased blood–brain barrier	57
Field validation of the performance of paper-based tests for the detection of the Zika and chikungunya viruses in serum samples	56
Antigen-bearing outer membrane vesicles as tumour vaccines produced in situ by ingested genetically engineered bacteria	56
One-day rapid sterility test for human-derived biopharmaceuticals	56
Neutrophils bearing adhesive polymer micropatches as a drug-free cancer immunotherapy	54
Antitumour vaccination via the targeted proteolysis of antigens isolated from tumour lysates	53
Generation of neural organoids for spinal-cord regeneration via the direct reprogramming of human astrocytes	52
How to make cover letters instructive	51
Seeing ageing through retinal images	51
Publisher Correction: Non-invasive characterization of pericyte dysfunction in mouse brain using functional ultrasound localization microscopy	51
Towards handy neural prostheses	51
Overcoming immune hurdles to implant longevity	50
Continuous insulin monitoring using an antibody-protecting zwitterionic microneedle patch	49
A generalist foundation model and database for open-world medical image segmentation	48
Author Correction: Targeted tumour theranostics in mice via carbon quantum dots structurally mimicking large amino acids	47
Author Correction: Mannich reaction-based combinatorial libraries identify antioxidant ionizable lipids for mRNA delivery with reduced immunogenicity	47
Optimization of base editors for the functional correction of SMN2 as a treatment for spinal muscular atrophy	47
A low-power stretchable neuromorphic nerve with proprioceptive feedback	46
Patterned gastrointestinal monolayers with bilateral access as observable models of parasite gut infection	46
Designing stable humanized antibodies	46
Fluorescence lifetime of injected indocyanine green as a universal marker of solid tumours in patients	46
Nutritional deficiency in an intestine-on-a-chip recapitulates injury hallmarks associated with environmental enteric dysfunction	46
Control of the antitumour activity and specificity of CAR T cells via organic adapters covalently tethering the CAR to tumour cells	46
Mapping and targeted viral activation of pancreatic nerves in mice reveal their roles in the regulation of glucose metabolism	46
Identification of druggable regulators of cell secretion via a kinome-wide screen and high-throughput immunomagnetic cell sorting	45
Aberrant chromatin reorganization in cells from diseased fibrous connective tissue in response to altered chemomechanical cues	45
Immunization against Zika by entrapping live virus in a subcutaneous self-adjuvanting hydrogel	44
Targeted knock-ins with pseudovirus for the stable expression of large transgenes	43
A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs	43
DNA-based computation for multiple biomarkers	43
Rejuvenation of tumour-specific T cells through bispecific antibodies targeting PD-L1 on dendritic cells	43

Thermoreversibly assembled polymersomes for highly efficient loading, processing and delivery of protein and siRNA biologics	42
Cas9-induced targeted integration of large DNA payloads in primary human T cells via homology-mediated end-joining DNA repair	42
Contextual learning is nearly all you need	42
Artificial intelligence advances skull stripping across lifespan	41
Stimulating localizable sensations	40
Macrophage-augmented organoids recapitulate the complex pathophysiology of viral diseases and enable development of multitarget therapeutics	38
Quantification of intratumoural heterogeneity in mice and patients via machine-learning models trained on PET–MRI data	38
Arrayed CRISPR libraries for the genome-wide activation, deletion and silencing of human protein-coding genes	38
Antimicrobial peptides boosted by ultrasound	38
Dynamical flexible inference of nonlinear latent factors and structures in neural population activity	38
Treatment of a severe vascular disease using a bespoke CRISPR–Cas9 base editor in mice	38
Clinical validation of a wearable ultrasound sensor of blood pressure	37
A swallowable X-ray dosimeter for the real-time monitoring of radiotherapy	37
Endocisternal interfaces for minimally invasive neural stimulation and recording of the brain and spinal cord	36
Assessing the toxicity of bispecific antibodies	36
A deep generative model for deciphering cellular dynamics and in silico drug discovery in complex diseases	36
A swallowable X-ray dosimeter	36
A fast all-optical 3D photoacoustic scanner for clinical vascular imaging	35
Minimally invasive implantation of scalable high-density cortical microelectrode arrays for multimodal neural decoding and stimulation	35
Hormonally mediated mechanical remodelling of human haematopoietic stem cells enhances their bone-marrow engraftment	35
Intradermally delivered mRNA-encapsulating extracellular vesicles for collagen-replacement therapy	34
Elimination of acquired resistance to PD-1 blockade via the concurrent depletion of tumour cells and immunosuppressive cells	34
Streamlined detection of SARS-CoV-2 via Cas13	34
A thermogalvanic cell dressing for smart wound monitoring and accelerated healing	34
Label-free intraoperative histology of bone tissue via deep-learning-assisted ultraviolet photoacoustic microscopy	34
Localized butyrate restores gut homeostasis	33
Making CRISPR more deliverable	33
Reinforcement of the intestinal mucosal barrier via mucus-penetrating PEGylated bacteria	33
Dynamic monitoring of thousands of biochemical analytes using microsampling	33
Safer and efficient base editing and prime editing via ribonucleoproteins delivered through optimized lipid-nanoparticle formulations	32
Transthoracic ultrasound localization microscopy of myocardial vasculature in patients	32
Unconditional latent diffusion models memorize patient imaging data	32
The analgesic potential of heavy water	32
Efficient recovery of potent tumour-infiltrating lymphocytes through quantitative immunomagnetic cell sorting	31
Accelerated sterility testing unlocks safe delivery of life-saving therapies	31
Immunogenic amines on lipid nanoparticles	31
Intraoperative imaging in pathology-assisted surgery	30
A stealthy neural recorder for the study of behaviour in primates	30
Benchmarking foundation models as feature extractors for weakly supervised computational pathology	30
CRISPR-GPT for agentic automation of gene-editing experiments	30
Screening oral drugs for their interactions with the intestinal transportome via porcine tissue explants and machine learning	29
Multiplexed discrimination of SARS-CoV-2 variants via plasmonic-enhanced fluorescence in a portable and automated device	29
A compact, wireless system for continuous monitoring of breast milk expressed during breastfeeding	29
Rapid discovery of high-affinity antibodies via massively parallel sequencing, ribosome display and affinity screening	29
Genome-wide Cas9-mediated screening of essential non-coding regulatory elements via libraries of paired single-guide RNAs	29
A wireless patch for the monitoring of C-reactive protein in sweat	28
Squeezing more out from clinical imaging	28
Paired CRISPR screening libraries for studying the function of the non-coding genome at scale	28
Expanded toolkits for RNA circularization	28
A generalist model for enhancing brain MRIs	28
A wearable electrochemical biosensor for the monitoring of metabolites and nutrients	28
Bridging scales in brain connectivity	28
CCR5-targeted allogeneic gamma–delta CD19 chimeric antigen receptor T cells for HIV-associated B cell-malignancy immunotherapy	27
Gene correction for sickle cell disease hits its prime	27
Sensing the DNA-mismatch tolerance of catalytically inactive Cas9 via barcoded DNA nanostructures in solid-state nanopores	27
Nitroglycerin-responsive gene switch for the on-demand production of therapeutic proteins	27
Targeting NK2R reverses cardiometabolic dysfunction	27
A HO-1 gene knockout using a NanoCRISPR scaffold suppresses metastasis in mouse models	27
An artificial cilia-based array system for sound frequency decoding and resonance-responsive drug release	27
Advanced glycation end-products as mediators of the aberrant crosslinking of extracellular matrix in scarred liver tissue	26
Holographic transcranial ultrasound neuromodulation enhances stimulation efficacy by cooperatively recruiting distributed brain circuits	26
Solvent-mediated analgesia via the suppression of water permeation through TRPV1 ion channels	26
Urinary bioorthogonal reporters for the monitoring of the efficacy of chemotherapy for lung cancer and of associated kidney injury	26
‘CRISPRing’ with therapeutic cells	25
Mapping light distribution in the brain via MRI	25
Author Correction: Organ-specific metastases obtained by culturing colorectal cancer cells on tissue-specific decellularized scaffolds	25
Nanoprobes for X-ray-mediated theranostics	25
Genetically engineered and enucleated human mesenchymal stromal cells for the targeted delivery of therapeutics to diseased tissue	25
Advancing breast cancer risk stratification using multimodal AI	25
Fast capturing of deep blood flow	25
Activatable near-infrared probes for the detection of specific populations of tumour-infiltrating leukocytes in vivo and in urine	25
Panoramic photoacoustic computed tomography with learning-based classification enhances breast lesion characterization	24
Quantitative imaging of apoptosis following oncolytic virotherapy by magnetic resonance fingerprinting aided by deep learning	24
Spatially resolved in vivo imaging of inflammation-associated mRNA via enzymatic fluorescence amplification in a molecular beacon	24
Engineering of a bespoke CRISPR base editor to treat a severe vascular disease	24
Tackling prediction uncertainty in machine learning for healthcare	23
Self-organization of sinusoidal vessels in pluripotent stem cell-derived human liver bud organoids	23
High-throughput spatiotemporal monitoring of single-cell secretions via plasmonic microwell arrays	23
A deep learning system for detecting silent brain infarction and predicting stroke risk	23
Closed-loop enhancement and neural decoding of cognitive control in humans	22
AAV-mediated delivery of a Sleeping Beauty transposon and an mRNA-encoded transposase for the engineering of therapeutic immune cells	22
Restoration of sensory feedback from the foot and reduction of phantom limb pain via closed-loop spinal cord stimulation	22
Oncolytic mineralized bacteria as potent locally administered immunotherapeutics	22
Generation of functionally distinct T-cell populations by altering the viscoelasticity of their extracellular matrix	22
Suppression of cytokine release syndrome during CAR-T-cell therapy via a subcutaneously injected interleukin-6-adsorbing hydrogel	22
Author Correction: An organoid-based screen for epigenetic inhibitors that stimulate antigen presentation and potentiate T-cell-mediated cytotoxicity	21
A multi-organ chip with matured tissue niches linked by vascular flow	21
Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases and prime editing	21
An instrument-free, programmable approach for nucleic acid detection	21
Histopathological biomarkers for predicting the tumour accumulation of nanomedicines	21
Targeting the PIEZO1 pathway boosts T cell antitumour cytotoxicity	21
Diagnosis of paediatric tuberculosis by optically detecting two virulence factors on extracellular vesicles in blood samples	21
Optimization of therapeutic antibodies for reduced self-association and non-specific binding via interpretable machine learning	21
Pathological mechanosensing	20
Setting morphogen gradients	20