Nature Biomedical Engineering

Article	Citations
Author Correction: Optogenetic stimulation of vagal nerves for enhanced glucose-stimulated insulin secretion and β cell proliferation	815
A humanized minipig model for the toxicological testing of therapeutic recombinant antibodies	511
Synaptic connectivity mapping among thousands of neurons via parallelized intracellular recording with a microhole electrode array	426
Rapid and stain-free quantification of viral plaque via lens-free holography and deep learning	358
Induction of antigen-specific tolerance by nanobody–antigen adducts that target class-II major histocompatibility complexes	318
A data-efficient strategy for building high-performing medical foundation models	314
Deep mutational learning for the selection of therapeutic antibodies resistant to the evolution of Omicron variants of SARS-CoV-2	306
Author Correction: Prolonged activation of innate immune pathways by a polyvalent STING agonist	297
An implantable hydrogel-based phononic crystal for continuous and wireless monitoring of internal tissue strains	276
A nanoparticle probe for the imaging of autophagic flux in live mice via magnetic resonance and near-infrared fluorescence	267
Systemic immune responses to irradiated tumours via the transport of antigens to the tumour periphery by injected flagellate bacteria	247
An integrated magneto-electrochemical device for the rapid profiling of tumour extracellular vesicles from blood plasma	243
Sensitive detection of SARS-CoV-2 on paper	227
Solid stress impairs lymphocyte infiltration into lymph-node metastases	216
A human-airway-on-a-chip for the rapid identification of candidate antiviral therapeutics and prophylactics	208
A soft and transparent contact lens for the wireless quantitative monitoring of intraocular pressure	203
A strain-programmed patch for the healing of diabetic wounds	201
Adaptive adversarial neural networks for the analysis of lossy and domain-shifted datasets of medical images	194
Linking the genotypes and phenotypes of cancer cells in heterogenous populations via real-time optical tagging and image analysis	193
Modelling ciliopathy phenotypes in human tissues derived from pluripotent stem cells with genetically ablated cilia	189
Large DNA deletions occur during DNA repair at 20-fold lower frequency for base editors and prime editors than for Cas9 nucleases	183
Unbiased discovery of autoantibodies associated with severe COVID-19 via genome-scale self-assembled DNA-barcoded protein libraries	182
Biomaterial-induced conversion of quiescent cardiomyocytes into pacemaker cells in rats	182
Publisher Correction: Restoration of sensory feedback from the foot and reduction of phantom limb pain via closed-loop spinal cord stimulation	181
Downregulating human leucocyte antigens on mesenchymal stromal cells by epigenetically repressing a β2-microglobulin super-enhancer	179

FAS-less allogeneic CAR T cells	172
Author Correction: Endocisternal interfaces for minimally invasive neural stimulation and recording of the brain and spinal cord	172
Streamlining point-of-care assays	171
Optogenetic stimulation of vagal nerves for enhanced glucose-stimulated insulin secretion and β cell proliferation	169
Amelioration of post-traumatic osteoarthritis via nanoparticle depots delivering small interfering RNA to damaged cartilage	169
Cas9-mediated replacement of expanded CAG repeats in a pig model of Huntington’s disease	166
A high-density microfluidic bioreactor for the automated manufacturing of CAR T cells	165
Intratumourally injected alum-tethered cytokines elicit potent and safer local and systemic anticancer immunity	162
Fast and sensitive electromechanical sensing	161
Designing receptor agonists with enhanced pharmacokinetics by grafting macrocyclic peptides into fragment crystallizable regions	156
Simultaneous visualization of multiple radionuclides in vivo	155
Faster detection of somatic structural variants	153
Probing when dCas9 tolerates DNA mismatches	150
Prevention and treatment of peri-implant fibrosis by functionally inhibiting skeletal cells expressing the leptin receptor	148
Offbeat drug-delivery tactics	145
An organoid-based screen for epigenetic inhibitors that stimulate antigen presentation and potentiate T-cell-mediated cytotoxicity	144
Masking the immunotoxicity of interleukin-12 by fusing it with a domain of its receptor via a tumour-protease-cleavable linker	143
Engineered allogeneic T cells decoupling T-cell-receptor and CD3 signalling enhance the antitumour activity of bispecific antibodies	143
A base editor for the long-term restoration of auditory function in mice with recessive profound deafness	142
Making fitter cells and tissues	140
Generation of antigen-specific mature T cells from RAG1−/−RAG2−/−B2M−/− stem cells by engineering their microenvironment	135
Augmented lipid-nanoparticle-mediated in vivo genome editing in the lungs and spleen by disrupting Cas9 activity in the liver	134
A deep-learning model for quantifying circulating tumour DNA from the density distribution of DNA-fragment lengths	133
Immune-privileged tissues formed from immunologically cloaked mouse embryonic stem cells survive long term in allogeneic hosts	131
A knockout run for CRISPRed cells	130
A two-phase point-of-care diagnostic device for bladder cancer	129
Subcutaneous pancreatic islet transplantation without immunosuppression	127
Sensing haemodynamics via wearables in sync	127
Challenges and opportunities of acquiring cortical recordings for chronic adaptive deep brain stimulation	124
Making MRI increasingly useful	123
Reprogramming of fibroblasts into expandable cardiovascular progenitor cells via small molecules in xeno-free conditions	121
An off-the-shelf otoacoustic-emission probe for hearing screening via a smartphone	121
Targeting vaccines to dendritic cells by mimicking the processing and presentation of antigens in xenotransplant rejection	119
1H magnetic resonance spectroscopic imaging of deuterated glucose and of neurotransmitter metabolism at 7 T in the human brain	118
Reduction of choroidal neovascularization via cleavable VEGF antibodies conjugated to exosomes derived from regulatory T cells	118
A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples	116
A paper-based assay for the colorimetric detection of SARS-CoV-2 variants at single-nucleotide resolution	114
Mapping light distribution in tissue by using MRI-detectable photosensitive liposomes	111
A bilingual speech neuroprosthesis driven by cortical articulatory representations shared between languages	108
Mapping tissue heterogeneity in solid tumours using PET–MRI and machine learning	108
Machine learning in translation	106
Straightforward, inexpensive and sensitive	104
Mutation corrections in spinal muscular atrophy	103
Allometrically scaling tissue forces drive pathological foreign-body responses to implants via Rac2-activated myeloid cells	101
Deep screening of antibody–antigen affinities	100
Synthetic cationic helical polypeptides for the stimulation of antitumour innate immune pathways in antigen-presenting cells	100
Publisher Correction: Efficient in vivo base editing via single adenoassociated viruses with size-optimized genomes encoding compact adenine base editors	99
Author Correction: Restoration of tumour-growth suppression in vivo via systemic nanoparticle-mediated delivery of PTENmRNA	99
Eyeing severe diabetes upfront	99
Ablation of FAS confers allogeneic CD3– CAR T cells with resistance to rejection by T cells and natural killer cells	98

Isolation of tumour-reactive lymphocytes from peripheral blood via microfluidic immunomagnetic cell sorting	98
Targeted desialylation and cytolysis of tumour cells by fusing a sialidase to a bispecific T-cell engager	97
A serine-conjugated butyrate prodrug with high oral bioavailability suppresses autoimmune arthritis and neuroinflammation in mice	95
Enhancement of the viability of T cells electroporated with DNA via osmotic dampening of the DNA-sensing cGAS–STING pathway	93
Screening for lipid nanoparticles that modulate the immune activity of helper T cells towards enhanced antitumour activity	92
CAR T cells expressing a bacterial virulence factor trigger potent bystander antitumour responses in solid cancers	90
Rapid adaptation of brain–computer interfaces to new neuronal ensembles or participants via generative modelling	89
An injectable subcutaneous colon-specific immune niche for the treatment of ulcerative colitis	89
Opportunities and challenges in the development of exoskeletons for locomotor assistance	88
Peptide-mediated delivery of CRISPR enzymes for the efficient editing of primary human lymphocytes	87
A battery-less wireless implant for the continuous monitoring of vascular pressure, flow rate and temperature	85
Profiling protein–protein interactions to predict the efficacy of B-cell-lymphoma-2-homology-3 mimetics for acute myeloid leukaemia	84
Osteopontin attenuates the foreign-body response to silicone implants	84
Induction and expansion of human PRRX1+ limb-bud-like mesenchymal cells from pluripotent stem cells	82
Insulin independence from stem-cell-derived islets	78
Explaining counterfactual images	78
Author Correction: Generation of model tissues with dendritic vascular networks via sacrificial laser-sintered carbohydrate templates	78
Author Correction: Thermally sentient bionic limbs	77
Addendum: A tissue-engineered scale model of the heart ventricle	77
Author Correction: Enhancing the immunogenicity of lipid-nanoparticle mRNA vaccines by adjuvanting the ionizable lipid and the mRNA	77
Concurrently depleting regulatory T cells and activating cytotoxic T cells in tumours	76
Antibiotic-mediated selection of randomly mutagenized and cytokine-expressing oncolytic viruses	74
Mineralized bacteria as an immunotherapy agent against various cancer types	73
Low-cost and scalable projected light-sheet microscopy for the high-resolution imaging of cleared tissue and living samples	73
Spatial multi-omics at subcellular resolution via high-throughput in situ pairwise sequencing	72
Potent prophylactic cancer vaccines harnessing surface antigens shared by tumour cells and induced pluripotent stem cells	71
Opportunities and challenges in cardiac tissue engineering from an analysis of two decades of advances	71
Multi-antigen spherical nucleic acid cancer vaccines	70
A prototype closed-loop brain–machine interface for the study and treatment of pain	69
Mining for encrypted peptide antibiotics in the human proteome	67
Rational immunosilencing of a promiscuous T-cell epitope in the capsid of an adeno-associated virus	66
Generation of T-cell-receptor-negative CD8αβ-positive CAR T cells from T-cell-derived induced pluripotent stem cells	65
Synthetic data in machine learning for medicine and healthcare	64
Enhanced intratumoural activity of CAR T cells engineered to produce immunomodulators under photothermal control	64
Synthetic libraries of immune cells displaying a diverse repertoire of chimaeric antigen receptors as a potent cancer immunotherapy	63
Write accessibly	62
Base editing ablates all protein-coding genes of the mitochondrial genome in mouse cells	62
Tooling up against mechanobiological orthodoxy	62
Publisher Correction: Targeting overexpressed antigens in glioblastoma via CAR T cells with computationally designed high-affinity protein binders	61
Antitumour vaccination via the targeted proteolysis of antigens isolated from tumour lysates	61
A window into solid stresses within tumours	61
Efficient production of non-immunogenic, long circular RNAs for high protein yield	59
Efficient circular RNA synthesis for potent rolling circle translation	59
Field validation of the performance of paper-based tests for the detection of the Zika and chikungunya viruses in serum samples	59
Neutrophils bearing adhesive polymer micropatches as a drug-free cancer immunotherapy	58
Derivation of prognostic contextual histopathological features from whole-slide images of tumours via graph deep learning	58
Antigen-bearing outer membrane vesicles as tumour vaccines produced in situ by ingested genetically engineered bacteria	57
Meta-analysis of the make-up and properties of in vitro models of the healthy and diseased blood–brain barrier	57
Generation of neural organoids for spinal-cord regeneration via the direct reprogramming of human astrocytes	56
Single-cell analysis of ductal differentiation	55
How to make cover letters instructive	55
Control of the antitumour activity and specificity of CAR T cells via organic adapters covalently tethering the CAR to tumour cells	55
Towards handy neural prostheses	55
Mapping and targeted viral activation of pancreatic nerves in mice reveal their roles in the regulation of glucose metabolism	54
Overcoming immune hurdles to implant longevity	54
Seeing ageing through retinal images	54
Author Correction: Targeted tumour theranostics in mice via carbon quantum dots structurally mimicking large amino acids	54
Identification of druggable regulators of cell secretion via a kinome-wide screen and high-throughput immunomagnetic cell sorting	53
Single-cell-resolved differentiation of human induced pluripotent stem cells into pancreatic duct-like organoids on a microwell chip	53
Patterned gastrointestinal monolayers with bilateral access as observable models of parasite gut infection	53
Lipid nanoparticles for the inhalation of mRNA	53
Rejuvenation of tumour-specific T cells through bispecific antibodies targeting PD-L1 on dendritic cells	52
Aberrant chromatin reorganization in cells from diseased fibrous connective tissue in response to altered chemomechanical cues	52
Optimization of base editors for the functional correction of SMN2 as a treatment for spinal muscular atrophy	51
T cells armed with C-X-C chemokine receptor type 6 enhance adoptive cell therapy for pancreatic tumours	51
Designing stable humanized antibodies	51
DNA-based computation for multiple biomarkers	51
Wirelessly operated bioelectronic sutures for the monitoring of deep surgical wounds	51
Nutritional deficiency in an intestine-on-a-chip recapitulates injury hallmarks associated with environmental enteric dysfunction	51
Immunization against Zika by entrapping live virus in a subcutaneous self-adjuvanting hydrogel	50
A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs	50
Fluorescence lifetime of injected indocyanine green as a universal marker of solid tumours in patients	50
A low-power stretchable neuromorphic nerve with proprioceptive feedback	49
Continuous monitoring of deep-tissue haemodynamics with stretchable ultrasonic phased arrays	49
Stimulating localizable sensations	49
Targeted knock-ins with pseudovirus for the stable expression of large transgenes	48
Arrayed CRISPR libraries for the genome-wide activation, deletion and silencing of human protein-coding genes	48
Streamlined detection of SARS-CoV-2 via Cas13	48
Endocisternal interfaces for minimally invasive neural stimulation and recording of the brain and spinal cord	47
Quantification of intratumoural heterogeneity in mice and patients via machine-learning models trained on PET–MRI data	47

Contextual learning is nearly all you need	47
Assessing the toxicity of bispecific antibodies	47
A swallowable X-ray dosimeter	46
Cas9-induced targeted integration of large DNA payloads in primary human T cells via homology-mediated end-joining DNA repair	46
Dynamical flexible inference of nonlinear latent factors and structures in neural population activity	45
Hormonally mediated mechanical remodelling of human haematopoietic stem cells enhances their bone-marrow engraftment	45
Clinical validation of a wearable ultrasound sensor of blood pressure	45
Rapid single-molecule detection of COVID-19 and MERS antigens via nanobody-functionalized organic electrochemical transistors	44
Localized butyrate restores gut homeostasis	44
Reinforcement of the intestinal mucosal barrier via mucus-penetrating PEGylated bacteria	43
A swallowable X-ray dosimeter for the real-time monitoring of radiotherapy	43
Elimination of acquired resistance to PD-1 blockade via the concurrent depletion of tumour cells and immunosuppressive cells	43
A fast all-optical 3D photoacoustic scanner for clinical vascular imaging	42
Intradermally delivered mRNA-encapsulating extracellular vesicles for collagen-replacement therapy	42
Label-free intraoperative histology of bone tissue via deep-learning-assisted ultraviolet photoacoustic microscopy	42
Making CRISPR more deliverable	42
Dynamic monitoring of thousands of biochemical analytes using microsampling	42
Rapid discovery of high-affinity antibodies via massively parallel sequencing, ribosome display and affinity screening	42
Safer and efficient base editing and prime editing via ribonucleoproteins delivered through optimized lipid-nanoparticle formulations	41
Efficient recovery of potent tumour-infiltrating lymphocytes through quantitative immunomagnetic cell sorting	41
Inhibition of aberrant tissue remodelling by mesenchymal stromal cells singly coated with soft gels presenting defined chemomechanical cues	40
Screening oral drugs for their interactions with the intestinal transportome via porcine tissue explants and machine learning	40
A stealthy neural recorder for the study of behaviour in primates	39
Genome-wide Cas9-mediated screening of essential non-coding regulatory elements via libraries of paired single-guide RNAs	39
Multiplexed discrimination of SARS-CoV-2 variants via plasmonic-enhanced fluorescence in a portable and automated device	38
Immunogenic amines on lipid nanoparticles	38
Transthoracic ultrasound localization microscopy of myocardial vasculature in patients	38
The analgesic potential of heavy water	38
A wireless patch for the monitoring of C-reactive protein in sweat	37
Squeezing more out from clinical imaging	37
Intraoperative imaging in pathology-assisted surgery	37
A wearable electrochemical biosensor for the monitoring of metabolites and nutrients	37
Bridging scales in brain connectivity	36
Targeting NK2R reverses cardiometabolic dysfunction	35
Refresh cell culture	34
Paired CRISPR screening libraries for studying the function of the non-coding genome at scale	34
Gene correction for sickle cell disease hits its prime	33
Expanded toolkits for RNA circularization	33
Delivery of local anaesthetics by a self-assembled supramolecular system mimicking their interactions with a sodium channel	32
Regeneration of infarcted mouse hearts by cardiovascular tissue formed via the direct reprogramming of mouse fibroblasts	32
Solvent-mediated analgesia via the suppression of water permeation through TRPV1 ion channels	32
Advanced glycation end-products as mediators of the aberrant crosslinking of extracellular matrix in scarred liver tissue	32
Nitroglycerin-responsive gene switch for the on-demand production of therapeutic proteins	32
A generalist model for enhancing brain MRIs	32
Sensing the DNA-mismatch tolerance of catalytically inactive Cas9 via barcoded DNA nanostructures in solid-state nanopores	31
Application of prime editing to the correction of mutations and phenotypes in adult mice with liver and eye diseases	30
Activatable near-infrared probes for the detection of specific populations of tumour-infiltrating leukocytes in vivo and in urine	30
Therapeutic implications of transplanted-cell death	30
Genetically engineered and enucleated human mesenchymal stromal cells for the targeted delivery of therapeutics to diseased tissue	30
Conjugation of glucosylated polymer chains to checkpoint blockade antibodies augments their efficacy and specificity for glioblastoma	30
Urinary bioorthogonal reporters for the monitoring of the efficacy of chemotherapy for lung cancer and of associated kidney injury	30
Advancing breast cancer risk stratification using multimodal AI	29
Shear mechanosensing drives tendon adaptation	29
Author Correction: Organ-specific metastases obtained by culturing colorectal cancer cells on tissue-specific decellularized scaffolds	28
Fast capturing of deep blood flow	28
Mapping light distribution in the brain via MRI	28
‘CRISPRing’ with therapeutic cells	28
Targeting the PIEZO1 pathway boosts T cell antitumour cytotoxicity	28
Closed-loop enhancement and neural decoding of cognitive control in humans	28
Nanoprobes for X-ray-mediated theranostics	28
Author Correction: Large-scale generation of functional mRNA-encapsulating exosomes via cellular nanoporation	28
Restoration of sensory feedback from the foot and reduction of phantom limb pain via closed-loop spinal cord stimulation	27
Tackling prediction uncertainty in machine learning for healthcare	27
Quantitative imaging of apoptosis following oncolytic virotherapy by magnetic resonance fingerprinting aided by deep learning	27
High-throughput spatiotemporal monitoring of single-cell secretions via plasmonic microwell arrays	27
Generation of functionally distinct T-cell populations by altering the viscoelasticity of their extracellular matrix	27
AAV-mediated delivery of a Sleeping Beauty transposon and an mRNA-encoded transposase for the engineering of therapeutic immune cells	27
Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases and prime editing	26
Suppression of cytokine release syndrome during CAR-T-cell therapy via a subcutaneously injected interleukin-6-adsorbing hydrogel	26
A multi-organ chip with matured tissue niches linked by vascular flow	26
Histopathological biomarkers for predicting the tumour accumulation of nanomedicines	26
Spatially resolved in vivo imaging of inflammation-associated mRNA via enzymatic fluorescence amplification in a molecular beacon	26
Oncolytic mineralized bacteria as potent locally administered immunotherapeutics	26
Diagnosis of paediatric tuberculosis by optically detecting two virulence factors on extracellular vesicles in blood samples	26
Cryomicroneedles for transdermal cell delivery	26
Optimization of therapeutic antibodies for reduced self-association and non-specific binding via interpretable machine learning	26
Setting morphogen gradients	25
Author Correction: A low-power stretchable neuromorphic nerve with proprioceptive feedback	25
The myriad ways to engineer cells	25
Author Correction: An organoid-based screen for epigenetic inhibitors that stimulate antigen presentation and potentiate T-cell-mediated cytotoxicity	25
An instrument-free, programmable approach for nucleic acid detection	25
Publisher Correction: Compression and amplification algorithms in hearing aids impair the selectivity of neural responses to speech	25
Textile-embedded cell-free biosensors	24
Fast volumetric scanning of living tissue	24
Cas12a-knock-in mice for multiplexed genome editing, disease modelling and immune-cell engineering	24
A lentiviral vector for the production of T cells with an inducible transgene and a constitutively expressed tumour-targeting receptor	23
Detection of signs of disease in external photographs of the eyes via deep learning	23
Author Correction: Enhanced control of a brain–computer interface by tetraplegic participants via neural-network-mediated feature extraction	23
Prevention of vascular-allograft rejection by protecting the endothelial glycocalyx with immunosuppressive polymers	23
Surgical polarimetric endoscopy for the detection of laryngeal cancer	23
Oxonium ion scanning mass spectrometry for large-scale plasma glycoproteomics	23
Pathological mechanosensing	23
Deep brain stimulation by blood–brain-barrier-crossing piezoelectric nanoparticles generating current and nitric oxide under focused ultrasound	22
Multiple-instance learning of somatic mutations for the classification of tumour type and the prediction of microsatellite status	22
Learning spatial cellular motifs predictive of the responses of patients to cancer treatments	22