Nature Biomedical Engineering

Article	Citations
Sensitive detection of SARS-CoV-2 on paper	771
Author Correction: Optogenetic stimulation of vagal nerves for enhanced glucose-stimulated insulin secretion and β cell proliferation	539
An implantable hydrogel-based phononic crystal for continuous and wireless monitoring of internal tissue strains	527
A data-efficient strategy for building high-performing medical foundation models	504
Rapid and stain-free quantification of viral plaque via lens-free holography and deep learning	353
Deep mutational learning for the selection of therapeutic antibodies resistant to the evolution of Omicron variants of SARS-CoV-2	353
Human gastric multi-regional assembloids for functional parietal maturation and patient-specific modelling of antral foveolar hyperplasia	330
A strain-programmed patch for the healing of diabetic wounds	307
A nanoparticle probe for the imaging of autophagic flux in live mice via magnetic resonance and near-infrared fluorescence	298
A humanized minipig model for the toxicological testing of therapeutic recombinant antibodies	295
Brain–heart–eye axis revealed by multi-organ imaging genetics and proteomics	276
Single-nucleus transcriptomics of an engineered pig model reveals microglia–T cell interactions driving Huntington’s disease neurodegeneration	266
Neuroimaging endophenotypes reveal underlying mechanisms and genetic factors contributing to progression and development of four brain disorders	243
Perilesional neuromodulation replaces lost sensorimotor function in persons with spinal cord injury	238
Emergency delivery of particulate drugs by active ejection using in vivo wireless devices	234
Synaptic connectivity mapping among thousands of neurons via parallelized intracellular recording with a microhole electrode array	226
Low-input deep learning platform for citrullinated peptide identification, autoantigen discovery and rheumatoid arthritis treatment stratification	220
FAS-less allogeneic CAR T cells	219
Streamlining point-of-care assays	206
Autonomous ultrasound with UltraBot	205
Publisher Correction: Restoration of sensory feedback from the foot and reduction of phantom limb pain via closed-loop spinal cord stimulation	198
Author Correction: Endocisternal interfaces for minimally invasive neural stimulation and recording of the brain and spinal cord	196
Systematic functional screening of switchable aptamer beacon probes	189
A generative model uses healthy and diseased image pairs for pixel-level chest X-ray pathology localization	178
Optogenetic stimulation of vagal nerves for enhanced glucose-stimulated insulin secretion and β cell proliferation	173

Three-dimensional architecture of human bone marrow	164
Unbiased discovery of autoantibodies associated with severe COVID-19 via genome-scale self-assembled DNA-barcoded protein libraries	163
A sonosensitive diphenylalanine-based broad-spectrum antimicrobial peptide	153
Ultrasensitive and long-lasting bioluminescence immunoassay for point-of-care viral antigen detection	149
Downregulating human leucocyte antigens on mesenchymal stromal cells by epigenetically repressing a β2-microglobulin super-enhancer	149
Cas9-mediated replacement of expanded CAG repeats in a pig model of Huntington’s disease	148
A high-density microfluidic bioreactor for the automated manufacturing of CAR T cells	143
Large DNA deletions occur during DNA repair at 20-fold lower frequency for base editors and prime editors than for Cas9 nucleases	142
Faster detection of somatic structural variants	140
Generation of antigen-specific mature T cells from RAG1−/−RAG2−/−B2M−/− stem cells by engineering their microenvironment	138
A warm introduction	133
Offbeat drug-delivery tactics	132
Engineered non-expansive matrix for blood vessel organoid development and ischaemic stroke repair	130
Designing receptor agonists with enhanced pharmacokinetics by grafting macrocyclic peptides into fragment crystallizable regions	129
Probing when dCas9 tolerates DNA mismatches	126
Engineered allogeneic T cells decoupling T-cell-receptor and CD3 signalling enhance the antitumour activity of bispecific antibodies	123
Anchored random reverse primer sequencing for quantitative detection of novel gene fusions	123
Fluid shear stress activates a targetable mechano-metastatic cascade to promote medulloblastoma metastasis	123
Publisher Correction: Large-scale generation of iNK and CAR-iNK cells from CD34+ haematopoietic stem and progenitor cells for adoptive immunotherapy	118
A filamentary soft robotic probe for multimodal in utero monitoring of fetal health	118
LDL-binding IL-10 reduces vascular inflammation in atherosclerotic mice	118
A base editor for the long-term restoration of auditory function in mice with recessive profound deafness	117
Masking the immunotoxicity of interleukin-12 by fusing it with a domain of its receptor via a tumour-protease-cleavable linker	116
Immune-privileged tissues formed from immunologically cloaked mouse embryonic stem cells survive long term in allogeneic hosts	112
Prevention and treatment of peri-implant fibrosis by functionally inhibiting skeletal cells expressing the leptin receptor	108
A deep-learning model for quantifying circulating tumour DNA from the density distribution of DNA-fragment lengths	107
Targeting inflammation with chimeric antigen receptor macrophages using a signal switch	104
Potentiating cancer immunotherapies with modular albumin-hitchhiking nanobody–STING agonist conjugates	102
A knockout run for CRISPRed cells	100
A two-phase point-of-care diagnostic device for bladder cancer	97
Sensing haemodynamics via wearables in sync	95
Subcutaneous pancreatic islet transplantation without immunosuppression	93
A deep learning and large language hybrid workflow for omics interpretation	92
X-ray activated platinum complex induces DNA damage and enhances cancer immunotherapy through abscopal effect	88
A bilingual speech neuroprosthesis driven by cortical articulatory representations shared between languages	87
Shape-conformal porous frameworks for full coverage of neural organoids and high-resolution electrophysiology	86
Making MRI increasingly useful	85
1H magnetic resonance spectroscopic imaging of deuterated glucose and of neurotransmitter metabolism at 7 T in the human brain	85
A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples	84
mRNA delivery to the endometrium	84
A three-dimensional multi-modal foundation model for optical coherence tomography	82
Mapping light distribution in tissue by using MRI-detectable photosensitive liposomes	82
A paper-based assay for the colorimetric detection of SARS-CoV-2 variants at single-nucleotide resolution	81
An off-the-shelf otoacoustic-emission probe for hearing screening via a smartphone	81
Targeting vaccines to dendritic cells by mimicking the processing and presentation of antigens in xenotransplant rejection	79
Nanobody-based bispecific antibody engagers targeting CTLA-4 or PD-L1 for cancer immunotherapy	79
Challenges and opportunities of acquiring cortical recordings for chronic adaptive deep brain stimulation	79
Multiplex imaging of amyloid-β plaques dynamics in living brains with quinoline-malononitrile-based probes	79
Mapping tissue heterogeneity in solid tumours using PET–MRI and machine learning	78
Mutation corrections in spinal muscular atrophy	77

Engineered thoracic spinal cord organoids for transplantation after spinal cord injury	76
Deep screening of antibody–antigen affinities	76
Where the nanocarriers go	76
Publisher Correction: Efficient in vivo base editing via single adenoassociated viruses with size-optimized genomes encoding compact adenine base editors	76
In vivo base editing rescues liver pathophysiology and peroxisome dysfunction in a mouse model of Zellweger spectrum disorder	75
Peptide-mediated delivery of CRISPR enzymes for the efficient editing of primary human lymphocytes	75
Accelerating primer design for amplicon sequencing using large language model-powered agents	74
Ablation of FAS confers allogeneic CD3– CAR T cells with resistance to rejection by T cells and natural killer cells	74
Label-free tissue NIR-II autofluorescence imaging for visualization of human liver malignancy	73
Synthetic cationic helical polypeptides for the stimulation of antitumour innate immune pathways in antigen-presenting cells	72
Straightforward, inexpensive and sensitive	72
Screening for lipid nanoparticles that modulate the immune activity of helper T cells towards enhanced antitumour activity	69
Symbiotic transcatheter pacemaker for lifelong energy regeneration and therapeutic function in porcine disease model	69
An injectable subcutaneous colon-specific immune niche for the treatment of ulcerative colitis	68
Empowering AI data scientists using a multi-agent LLM framework with self-evolving capabilities for autonomous, tool-aware biomedical data analyses	68
A serine-conjugated butyrate prodrug with high oral bioavailability suppresses autoimmune arthritis and neuroinflammation in mice	67
Targeted desialylation and cytolysis of tumour cells by fusing a sialidase to a bispecific T-cell engager	66
Isolation of tumour-reactive lymphocytes from peripheral blood via microfluidic immunomagnetic cell sorting	65
Allometrically scaling tissue forces drive pathological foreign-body responses to implants via Rac2-activated myeloid cells	65
Opportunities and challenges in the development of exoskeletons for locomotor assistance	65
Profiling protein–protein interactions to predict the efficacy of B-cell-lymphoma-2-homology-3 mimetics for acute myeloid leukaemia	64
Author Correction: Restoration of tumour-growth suppression in vivo via systemic nanoparticle-mediated delivery of PTENmRNA	64
Enhancement of the viability of T cells electroporated with DNA via osmotic dampening of the DNA-sensing cGAS–STING pathway	63
Insulin independence from stem-cell-derived islets	62
A battery-less wireless implant for the continuous monitoring of vascular pressure, flow rate and temperature	62
Mineralized bacteria as an immunotherapy agent against various cancer types	61
Targeting myeloid cell-mediated fibrosis through FAK	60
Explaining counterfactual images	60
Concurrently depleting regulatory T cells and activating cytotoxic T cells in tumours	58
Author Correction: Thermally sentient bionic limbs	58
Osteopontin attenuates the foreign-body response to silicone implants	57
Engineering multi-specific nano-antibodies for cancer immunotherapy	57
The ever-changing communication of scientific discovery	57
On the horizon in biomedical engineering	57
Generation of T-cell-receptor-negative CD8αβ-positive CAR T cells from T-cell-derived induced pluripotent stem cells	56
Author Correction: Enhancing the immunogenicity of lipid-nanoparticle mRNA vaccines by adjuvanting the ionizable lipid and the mRNA	56
Multi-antigen spherical nucleic acid cancer vaccines	56
Antibiotic-mediated selection of randomly mutagenized and cytokine-expressing oncolytic viruses	55
A geometry aware framework enhances noninvasive mapping of whole human brain dynamics	54
Low-cost and scalable projected light-sheet microscopy for the high-resolution imaging of cleared tissue and living samples	54
Potent prophylactic cancer vaccines harnessing surface antigens shared by tumour cells and induced pluripotent stem cells	54
Rational immunosilencing of a promiscuous T-cell epitope in the capsid of an adeno-associated virus	53
Spatial multi-omics at subcellular resolution via high-throughput in situ pairwise sequencing	53
Automated disc device for multiplexed extracellular vesicle isolation and labelling from liquid biopsies in cancer diagnostics	52
Directed evolution-based discovery of ligands for in vivo restimulation of chimeric antigen receptor T cells	51
Synthetic libraries of immune cells displaying a diverse repertoire of chimaeric antigen receptors as a potent cancer immunotherapy	51
Tooling up against mechanobiological orthodoxy	50
Base editing ablates all protein-coding genes of the mitochondrial genome in mouse cells	50
Learning neuroimaging models from health system-scale data	50
Efficient production of non-immunogenic, long circular RNAs for high protein yield	49
Biomimetic model to study penile dysfunctions	49
Label-free navigation system for grading prostate tumour malignancy in situ via tissue pH and prostate-specific antigen activity	49
A window into solid stresses within tumours	49
Tumour-specific bioorthogonal synthesis of proteolysis-targeting chimeras and nanoparticles boosts T cell activity	48
One-day rapid sterility test for human-derived biopharmaceuticals	48
Antigen-bearing outer membrane vesicles as tumour vaccines produced in situ by ingested genetically engineered bacteria	48
Publisher Correction: Targeting overexpressed antigens in glioblastoma via CAR T cells with computationally designed high-affinity protein binders	48
Antitumour vaccination via the targeted proteolysis of antigens isolated from tumour lysates	47
Theoretical quantitative model and clinical outcome predictions of conductive cardiac patches for electrophysiological treatments	47
Precise virulence inactivation using a CRISPR-associated transposase for combating Enterobacteriaceae gut pathogens	46
Neutrophils bearing adhesive polymer micropatches as a drug-free cancer immunotherapy	46
Derivation of prognostic contextual histopathological features from whole-slide images of tumours via graph deep learning	45
Meta-analysis of the make-up and properties of in vitro models of the healthy and diseased blood–brain barrier	45
Efficient circular RNA synthesis for potent rolling circle translation	44
Seeing ageing through retinal images	43
Towards handy neural prostheses	43
How to make cover letters instructive	43
Generation of neural organoids for spinal-cord regeneration via the direct reprogramming of human astrocytes	43
Optimization of base editors for the functional correction of SMN2 as a treatment for spinal muscular atrophy	42
A low-power stretchable neuromorphic nerve with proprioceptive feedback	42
Immunization against Zika by entrapping live virus in a subcutaneous self-adjuvanting hydrogel	42
Patterned gastrointestinal monolayers with bilateral access as observable models of parasite gut infection	41
Mapping and targeted viral activation of pancreatic nerves in mice reveal their roles in the regulation of glucose metabolism	41
A generalist foundation model and database for open-world medical image segmentation	41
Continuous insulin monitoring using an antibody-protecting zwitterionic microneedle patch	41
Overcoming immune hurdles to implant longevity	40
Control of the antitumour activity and specificity of CAR T cells via organic adapters covalently tethering the CAR to tumour cells	40
Fluorescence lifetime of injected indocyanine green as a universal marker of solid tumours in patients	40
Identification of druggable regulators of cell secretion via a kinome-wide screen and high-throughput immunomagnetic cell sorting	40
A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs	40

Publisher Correction: Non-invasive characterization of pericyte dysfunction in mouse brain using functional ultrasound localization microscopy	40
Author Correction: Mannich reaction-based combinatorial libraries identify antioxidant ionizable lipids for mRNA delivery with reduced immunogenicity	40
Designing stable humanized antibodies	39
Whole cross-sectional human ultrasound tomography	39
DNA-based computation for multiple biomarkers	39
Aberrant chromatin reorganization in cells from diseased fibrous connective tissue in response to altered chemomechanical cues	39
Nutritional deficiency in an intestine-on-a-chip recapitulates injury hallmarks associated with environmental enteric dysfunction	38
Stimulating localizable sensations	37
Assessing the toxicity of bispecific antibodies	37
Artificial intelligence advances skull stripping across lifespan	37
A swallowable X-ray dosimeter	37
Targeted knock-ins with pseudovirus for the stable expression of large transgenes	37
Hormonally mediated mechanical remodelling of human haematopoietic stem cells enhances their bone-marrow engraftment	36
Localized butyrate restores gut homeostasis	36
Streamlined detection of SARS-CoV-2 via Cas13	36
Contextual learning is nearly all you need	36
Thermoreversibly assembled polymersomes for highly efficient loading, processing and delivery of protein and siRNA biologics	36
A fast all-optical 3D photoacoustic scanner for clinical vascular imaging	36
A deep generative model for deciphering cellular dynamics and in silico drug discovery in complex diseases	36
Label-free intraoperative histology of bone tissue via deep-learning-assisted ultraviolet photoacoustic microscopy	36
Macrophage-augmented organoids recapitulate the complex pathophysiology of viral diseases and enable development of multitarget therapeutics	35
Minimally invasive implantation of scalable high-density cortical microelectrode arrays for multimodal neural decoding and stimulation	35
Quantification of intratumoural heterogeneity in mice and patients via machine-learning models trained on PET–MRI data	35
Arrayed CRISPR libraries for the genome-wide activation, deletion and silencing of human protein-coding genes	35
Cas9-induced targeted integration of large DNA payloads in primary human T cells via homology-mediated end-joining DNA repair	35
Antimicrobial peptides boosted by ultrasound	35
A thermogalvanic cell dressing for smart wound monitoring and accelerated healing	34
Endocisternal interfaces for minimally invasive neural stimulation and recording of the brain and spinal cord	34
A swallowable X-ray dosimeter for the real-time monitoring of radiotherapy	34
Dynamical flexible inference of nonlinear latent factors and structures in neural population activity	34
Treatment of a severe vascular disease using a bespoke CRISPR–Cas9 base editor in mice	34
Clinical validation of a wearable ultrasound sensor of blood pressure	33
Making CRISPR more deliverable	33
Reinforcement of the intestinal mucosal barrier via mucus-penetrating PEGylated bacteria	33
Dynamic monitoring of thousands of biochemical analytes using microsampling	33
Intradermally delivered mRNA-encapsulating extracellular vesicles for collagen-replacement therapy	33
Accelerated sterility testing unlocks safe delivery of life-saving therapies	32
The analgesic potential of heavy water	32
Unconditional latent diffusion models memorize patient imaging data	31
Transthoracic ultrasound localization microscopy of myocardial vasculature in patients	31
A foundation generative model for breast ultrasound image analysis	31
Genome-wide Cas9-mediated screening of essential non-coding regulatory elements via libraries of paired single-guide RNAs	31
A compact, wireless system for continuous monitoring of breast milk expressed during breastfeeding	31
Multiplexed discrimination of SARS-CoV-2 variants via plasmonic-enhanced fluorescence in a portable and automated device	31
A vision–language pretrained transformer for versatile clinical respiratory disease applications	30
A stealthy neural recorder for the study of behaviour in primates	30
Benchmarking foundation models as feature extractors for weakly supervised computational pathology	30
Screening oral drugs for their interactions with the intestinal transportome via porcine tissue explants and machine learning	30
Rapid discovery of high-affinity antibodies via massively parallel sequencing, ribosome display and affinity screening	30
A wearable electrochemical biosensor for the monitoring of metabolites and nutrients	29
Confounding factors and biases abound when predicting molecular biomarkers from histological images	29
CRISPR-GPT for agentic automation of gene-editing experiments	29
Squeezing more out from clinical imaging	28
Predictive biomarkers of response to chimeric antigen receptor (CAR) T-cell therapy for pan-haematologic cancer	28
Safer and efficient base editing and prime editing via ribonucleoproteins delivered through optimized lipid-nanoparticle formulations	28
A wireless patch for the monitoring of C-reactive protein in sweat	28
Immunogenic amines on lipid nanoparticles	28
Paired CRISPR screening libraries for studying the function of the non-coding genome at scale	27
CCR5-targeted allogeneic gamma–delta CD19 chimeric antigen receptor T cells for HIV-associated B cell-malignancy immunotherapy	27
A generalist model for enhancing brain MRIs	27
Wireless and bioresorbable triboelectric nerve block system for postoperative pain control	27
Bridging scales in brain connectivity	27
Targeting NK2R reverses cardiometabolic dysfunction	27
Sensing the DNA-mismatch tolerance of catalytically inactive Cas9 via barcoded DNA nanostructures in solid-state nanopores	26
An artificial cilia-based array system for sound frequency decoding and resonance-responsive drug release	26
Building foundation models for cardiac MRI	26
Urinary bioorthogonal reporters for the monitoring of the efficacy of chemotherapy for lung cancer and of associated kidney injury	25
Generalist foundation models from a multimodal dataset for 3D computed tomography	25
A HO-1 gene knockout using a NanoCRISPR scaffold suppresses metastasis in mouse models	25
Activatable near-infrared probes for the detection of specific populations of tumour-infiltrating leukocytes in vivo and in urine	25
Advanced glycation end-products as mediators of the aberrant crosslinking of extracellular matrix in scarred liver tissue	25
Boosting prime editing with engineered non-canonical pegRNAs	25
Expanded toolkits for RNA circularization	25
Nitroglycerin-responsive gene switch for the on-demand production of therapeutic proteins	25
Gene correction for sickle cell disease hits its prime	25
Solvent-mediated analgesia via the suppression of water permeation through TRPV1 ion channels	25
Engineering of a bespoke CRISPR base editor to treat a severe vascular disease	24
Nanoprobes for X-ray-mediated theranostics	24
Holographic transcranial ultrasound neuromodulation enhances stimulation efficacy by cooperatively recruiting distributed brain circuits	24
Advancing breast cancer risk stratification using multimodal AI	24
Fast capturing of deep blood flow	24
Author Correction: Organ-specific metastases obtained by culturing colorectal cancer cells on tissue-specific decellularized scaffolds	24
‘CRISPRing’ with therapeutic cells	24
Mapping light distribution in the brain via MRI	24
High-throughput spatiotemporal monitoring of single-cell secretions via plasmonic microwell arrays	23
Oncolytic mineralized bacteria as potent locally administered immunotherapeutics	23
Histopathological biomarkers for predicting the tumour accumulation of nanomedicines	23
Suppression of cytokine release syndrome during CAR-T-cell therapy via a subcutaneously injected interleukin-6-adsorbing hydrogel	23
A deep learning system for detecting silent brain infarction and predicting stroke risk	23
Optimization of therapeutic antibodies for reduced self-association and non-specific binding via interpretable machine learning	23
Targeting the PIEZO1 pathway boosts T cell antitumour cytotoxicity	22
Self-organization of sinusoidal vessels in pluripotent stem cell-derived human liver bud organoids	22
Generation of functionally distinct T-cell populations by altering the viscoelasticity of their extracellular matrix	22
AAV-mediated delivery of a Sleeping Beauty transposon and an mRNA-encoded transposase for the engineering of therapeutic immune cells	22
Restoration of sensory feedback from the foot and reduction of phantom limb pain via closed-loop spinal cord stimulation	22