OOIR: Observatory of International Research

Papers

(The TQCC of ACS Synthetic Biology is 11. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-04-01 to 2025-04-01.)

Article	Citations
Issue Editorial Masthead	96
A Computational Metabolic Model for Engineered Production of Resveratrol in Escherichia coli	74
LevSeq: Rapid Generation of Sequence-Function Data for Directed Evolution and Machine Learning	73
An Automated Cell-Free Workflow for Transcription Factor Engineering	73
Integrating CRISPR-Cas12a with a crRNA-Mediated Catalytic Network for the Development of a Modular and Sensitive Aptasensor	71
Saccharomyces cerevisiae Synthetic Transcriptional Networks Harnessing dCas12a and Type V-A anti-CRISPR Proteins	69
Cell Free Bacteriophage Synthesis from Engineered Strains Improves Yield	63
Integrating Deep Learning and Synthetic Biology: A Co-Design Approach for Enhancing Gene Expression via N-Terminal Coding Sequences	62
Equipping Saccharomyces cerevisiae with an Additional Redox Cofactor Allows F₄₂₀-Dependent Bioconversions in Yeast	58
Issue Publication Information	57
Issue Publication Information	56
Revolutionizing Point-of-Care Diagnostics via CRISPR Systems	55
Toward Minimal Transcription–Translation Machinery	53
Directed Evolution of a Genetically Encoded Indicator for Chloride	52
Coproduction of Phase-Separated Carotenoids and β-Farnesene as a Yeast Biomass Valorization Strategy	49
Targeting Transcriptional and Translational Hindrances in a Modular T7RNAP Expression System in Engineered Pseudomonas putida	49
Issue Editorial Masthead	49
Issue Editorial Masthead	49
Issue Editorial Masthead	48
Issue Publication Information	48
ACtivE: Assembly and CRISPR-Targeted in Vivo Editing for Yeast Genome Engineering Using Minimum Reagents and Time	47
Characterization of Eight Bacterial Biosensors for Microbial Diagnostic and Therapeutic Applications	46
In VivoSampling of Intracellular Heterogeneity ofPseudomonas putidaEnables Multiobjective Optimization of Genetic Devices	46
Synthetic Biology 2.0: The Dawn of a New Era	46
Pipetting Hand, Together	45

Issue Publication Information	45
Engineered Biosynthesis and Anticancer Studies of Ring-Expanded Antimycin-Type Depsipeptides	44
Issue Editorial Masthead	44
Correction to “Enabling Biological Nitrogen Fixation for Cereal Crops in Fertilized Fields”	44
Issue Publication Information	44
Correction to “Reprogramming the Biosynthesis of Precursor Peptide to Create a Selenazole-Containing Nosiheptide Analogue”	44
Issue Publication Information	43
Issue Editorial Masthead	43
Balancing the AspC and AspA Pathways of Escherichia coli by Systematic Metabolic Engineering Strategy for High-Efficient l-Homoserine Production	42
Creating RNA Specific C-to-U Editase from APOBEC3A by Separation of Its Activities on DNA and RNA Substrates	41
Metabolic Engineering of Aspartic Acid Supply Modules for Enhanced Production of Bacitracin in Bacillus licheniformis	39
Considerations for Domestication of Novel Strains of Filamentous Fungi	38
Phage Enzyme-Assisted Direct In Vivo DNA Assembly in Multiple Microorganisms	38
From De Novo to Xeno: Advancing Macromolecule Design beyond Proteins	38
Protease-Responsive Toolkit for Conditional Targeted Protein Degradation	38
Solving Challenges in Microalgae-Based Living Materials	37
DRIMS: A Synthetic Biology Platform that Enables Deletion, Replacement, Insertion, Mutagenesis, and Synthesis of DNA	37
Advancing VB₁₂ Production: Insights into Enhancing VB₁₂ Titer in Ensifer adhaerens Casida A through ARTP Mutagenesis and Multiomics Analysis	37
High-Yielding Terpene-Based Biofuel Production in Rhodobacter capsulatus	36
Escherichia coli Data-Driven Strain Design Using Aggregated Adaptive Laboratory Evolution Mutational Data	36
Reduction of the Bacterial Genome by Transposon-Mediated Random Deletion	36
IWBDA 2022: Toward a Modular Future of Synthetic Biology Driven by Bio-Design Automation	35
SynHEAL: Synthesis of Health Equity, Advancement, and Leadership	35
Issue Publication Information	34
Issue Publication Information	34
Modular Synthetic Biology Toolkit for Filamentous Fungi	33
Prebiotics-Controlled Disposable Engineered Bacteria for Intestinal Diseases	33
Pipette Show: An Open Source Web Application to Support Pipetting into Microplates	32
Combining Chemical Catalysis with Enzymatic Steps for the Synthesis of the Artemisinin Precursor Dihydroartemisinic Acid	32
Development of an Efficient Recombinant Protein Expression System in Clostridium saccharoperbutylacetonicum Based on the Bacteriophage T7 System	31
Correction to “Lustro: High-Throughput Optogenetic Experiments Enabled by Automation and a Yeast Optogenetic Toolkit”	31
Signal Amplification for Cell-Free Biosensors, an Analog-to-Digital Converter	31
Construction of an Engineered Escherichia coli with Efficient Chemotactic and Metabolizing Ability toward Tetrathionate	31
Scaling the Functional Nanopore (FuN) Screen: Systematic Evaluation of Self-Assembling Membrane Peptides and Extension with a K⁺-Responsive Fluorescent Protein Sensor	31
Construction of a Compact Array of Microplasma Jet Devices and Its Application for Random Mutagenesis of Rhodosporidium toruloides	31
Synthetic Genomics: Repurposing Biological Systems for Applications in Engineering Biology	31
Efficient Genome Editing in Most Staphylococcus aureus by Using the Restriction-Modification System Silent CRISPR-Cas9 Toolkit	31
MetJ-Based Mutually Interfering SAM-ON/SAM-OFF Biosensors	30
Scaling-up Engineering Biology for Enhanced Environmental Solutions	30
A Sirtuin-Dependent T7 RNA Polymerase Variant	30
Using Methyl Bromide for Interspecies Cell–Cell Signaling and As a Reporter in a Model Soil Consortium	30
Motility of Synthetic Cells from Engineered Lipids	30
Development of the Static and Dynamic Gene Expression Regulation Toolkit in Pseudomonas chlororaphis	30
Programming Dynamic Division of Labor Using Horizontal Gene Transfer	29
Efficient Strategy for Synthesizing Vector-Free and Oncolytic Herpes Simplex Type 1 Viruses	29
Engineering Tunable, Low Latency Spatial Computation with Dual Input Quorum Sensing Promoters	29
Regulatory Components for Bacterial Cell-Free Systems Engineering	28
Label-Free Detection of Transgenic Crops Using an Isothermal Amplification Reporting CRISPR/Cas12 Assay	28
Antibiotic-Efficient Genetic Cassette for the TEM-1 β-Lactamase That Improves Plasmid Performance	28
Ribosomal Protein S1 Improves the Protein Yield of an In Vitro Reconstituted Cell-Free Translation System	28

Critical Assessment of Protein Engineering (CAPE): A Student Challenge on the Cloud	28
Codon-Restrained Method for Both Eliminating and Creating Intragenic Bacterial Promoters	28
An Optimized Version of the Small Synthetic Genome (Mini-Synplastome) for Plastid Metabolic Engineering in Solanum tuberosum (Potato)	27
Optimization of Heterologous Glucoraphanin Production In Planta	26
Programmable Synthetic Upstream Activating Sequence Library for Fine-Tuning Gene Expression Levels in Saccharomyces cerevisiae	26
Adenine Base Editing System for Pseudomonas and Prediction Workflow for Protein Dysfunction via ABE	26
MIRA/PfAgo-Mediated Biosensor for Multiplex Human Enteroviruses Virus Typing Detection on HFMD	26
Experimental and Analytical Approaches for Improving the Resolution of Randomly Barcoded Transposon Insertion Sequencing (RB-TnSeq) Studies	26
Graph Computation Using Algorithmic Self-Assembly of DNA Molecules	26
Plasmid-Stabilizing Strains for Antibiotic-Free Chemical Fermentation	25
Transcription-Associated Fluorescence-Activated Droplet Sorting for Di-rhamnolipid Hyperproducers	25
Control Intracellular Protein Condensates with Light	25
Reconfiguring the Escherichia coli Electron Transport Chain to Enhance trans-2-Decenoic Acid Production	24
Engineering of the Ligand Specificity of Transcriptional Regulator XylS by Deep Mutational Scanning	24
Discovery of 8-Hydroxyquinoline as a Histamine Receptor 2 Blocker Scaffold	24
Glycodiversifying Testosterone with a Promiscuous Glycosyltransferase OsSGT2 from Ornithogalum saundersiae	24
Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based Applications, Genomic Integrations and Combinatorial Libraries	24
De Novo Production of Hydroxytyrosol by Saccharomyces cerevisiae–Escherichia coli Coculture Engineering	24
Control of λ Lysogenic Escherichia coli Cells by Synthetic λ Phage Carrying cI^antisense	24
Modeling Microbial Communities: Perspective and Challenges	24
Kinetics of RNA and RNA:DNA Hybrid Strand Displacement	24
Improved Cell-Free Transcription–Translation Reactions in Microfluidic Chemostats Augmented with Hydrogel Membranes for Continuous Small Molecule Dialysis	23
Desiccated Cyanobacteria Serve As Efficient Plasmid DNA Carriers in Space Flight	23
A Novel Membrane-Associated Protein Aids Bacterial Colonization of Maize	23
Engineered Bacillus subtilis as Oral Probiotics To Enhance Clearance of Blood Lactate	23
Direct Transfer and Consolidation of Synthetic Yeast Chromosomes by Abortive Mating and Chromosome Elimination	23
Synonymous Codons and Hydrophobicity Optimization of Post-translational Signal Peptide PelB Increase Phage Display Efficiency of DARPins	23
Impact of Chemical Dynamics of Commercial PURE Systems on Malachite Green Aptamer Fluorescence	23
Determination of Nucleotide Sequences within Promoter Regions Affecting Promoter Compatibility between Zymomonas mobilis and Escherichia coli	23
Optimizing a CRISPR-Cas13d Gene Circuit for Tunable Target RNA Downregulation with Minimal Collateral RNA Cutting	23
Engineering Yarrowia lipolytica to Produce Tailored Chain-Length Fatty Acids and Their Derivatives	23
Optical Control of Cell Signaling with Red/Far-Red Light-Responsive Optogenetic Tools in Caenorhabditis elegans	23
Optimized CRISPR Interference System for Investigating Pseudomonas alloputida Genes Involved in Rhizosphere Microbiome Assembly	23
Evaluating the Contribution of Model Complexity in Predicting Robustness in Synthetic Genetic Circuits	23
PeroxiHUB: A Modular Cell-Free Biosensing Platform Using H₂O₂ as Signal Integrator	23
Efficient CRISPR/Cas12a-Based Genome-Editing Toolbox for Metabolic Engineering in Methanococcus maripaludis	23
Mobile Translation Systems Generate Genomically Engineered Escherichia coli Cells with Improved Growth Phenotypes	23
Energy Aware Technology Mapping of Genetic Logic Circuits	22
Standard Intein Gene Expression Ramps (SIGER) for Protein-Independent Expression Control	22
The VEGAS Platform Is Unsuitable for Mammalian Directed Evolution	22
Discovering Content through Text Mining for a Synthetic Biology Knowledge System	22
Actomyosin-Driven Division of a Synthetic Cell	22
A Biological OR(XNOR) Logic Gate Couples Carbon Source and Transgene Expression Switching in a Komagataella phaffii (Pichia pastoris) Strain Co-producing Process-Enhancing Lipase and a V	22
Engineering Metabolic Pathways for Cofactor Self-Sufficiency and Serotonin Production in Escherichia coli	22
LOICA: Integrating Models with Data for Genetic Network Design Automation	22
Combinatorial Protein Engineering and Metabolic Engineering for Efficient Synthesis of l-Histidine in Corynebacterium glutamicum	22
Restructuring a Complex Genetic Function on Episomal Vectors in Escherichia coli	22
A Logically Reversible Double Feynman Gate with Molecular Engineered Bacteria Arranged in an Artificial Neural Network-Type Architecture	22
Metabolic Engineering of Corynebacterium glutamicum for the Production of the Four-Carbon Platform Chemicals γ-Hydroxybutyrate and γ-Butyrolactone	22
Programmable Acetylation Modification of Bacterial Proteins by a Cas12a-Guided Acetyltransferase	22
Engineering a Silk Protein-Mediated Customizable Compartment for Modular Metabolic Synthesis	22
Stem Loop Mediated Transgene Modulation in Human T Cells	22
Bacteria Engineered to Produce Serotonin Modulate Host Intestinal Physiology	22
Optimal LentiCRISPR-Based System for Sequential CRISPR/Cas9 Screens	21
Plasmid Copy Number Engineering Accelerates Fungal Polyketide Discovery upon Unnatural Polyketide Biosynthesis	21
Characterizing and Tailoring the Substrate Profile of a γ-Glutamyltransferase Variant	21
Easy Modular Integrative fuSion-ready Expression (Easy-MISE) Toolkit for Fast Engineering of Heterologous Productions in Saccharomyces cerevisiae	21
Dynamic Control Strategy to Produce Riboflavin with Lignocellulose Hydrolysate in the Thermophile Geobacillus thermoglucosidasius	21
Highly Accurate Sequence- and Position-Independent Error Profiling of DNA Synthesis and Sequencing	21
Parameterized Computational Framework for the Description and Design of Genetic Circuits of Morphogenesis Based on Contact-Dependent Signaling and Changes in Cell–Cell Adhesion	21
In Vivo Genome Editing in Type I and II Methanotrophs Using a CRISPR/Cas9 System	21
GENETTA: a Network-Based Tool for the Analysis of Complex Genetic Designs	21
Formation of Giant Lipid Vesicle Containing Dual Functions Facilitates Outer Membrane Phospholipase	21
Engineering of a TrpR-Based Biosensor for Altered Dynamic Range and Ligand Preference	21
Lustro: High-Throughput Optogenetic Experiments Enabled by Automation and a Yeast Optogenetic Toolkit	21
A Massively Parallel In Vivo Assay of TdT Mutants Yields Variants with Altered Nucleotide Insertion Biases	21
New Target Gene Screening Using Shortened and Random sgRNA Libraries in Microbial CRISPR Interference	21
Multiple Rounds of In Vivo Random Mutagenesis and Selection in Vibrio natriegens Result in Substantial Increases in REE Binding Capacity	21
Increasing MinD’s Membrane Affinity Yields Standing Wave Oscillations and Functional Gradients on Flat Membranes	20
DisGUVery: A Versatile Open-Source Software for High-Throughput Image Analysis of Giant Unilamellar Vesicles	20
Interaction between the Anchoring Domain of A-Kinase Anchoring Proteins and the Dimerization and Docking Domain of Protein Kinase A: A Potent Tool for Synthetic Biology	20
Two-Layered Microfluidic Devices for High-Throughput Dynamic Analysis of Synthetic Gene Circuits in E. coli	20
Performance Prediction of Fundamental Transcriptional Programs	20
Generation of a Live Attenuated Influenza A Vaccine Using Chemical-Triggered Intein	20
An Autocatalytic Peptide Cyclase Improves Fidelity and Yield of Circular Peptides In Vivo and In Vitro	20
In Vitro Transcription/Translation-Coupled DNA Replication through Partial Regeneration of 20 Aminoacyl-tRNA Synthetases	20
Inducible Synthetic Growth Regulation Using the ClpXP Proteasome Enhances cis,cis-Muconic Acid and Glycolic Acid Yields in Saccharomyces cerevisiae	20
Directed Evolution of Acoustic Reporter Genes Using High-Throughput Acoustic Screening	20
Engineering Agrobacterium tumefaciens Adhesion to Target Cells	20
Pyrococcus furiosus Argonaute Based Detection Assays for Porcine Deltacoronavirus	20

DNA Computing: NOT Logic Gates See the Light	20
Heterologous Single-Strand DNA-Annealing and Binding Protein Enhance CRISPR-Based Genome Editing Efficiency in Komagataella phaffii	20
Metabolic Tug-of-War between Glycolysis and Translation Revealed by Biochemical Reconstitution	19
Production and Characterization of Motile and Chemotactic Bacterial Minicells	19
Cowpea NAC Transcription Factors Positively Regulate Cellular Stress Response and Balance Energy Metabolism in Yeast via Reprogramming of Biosynthetic Pathways	19
Development of a Recombineering System for the Acetogen Eubacterium limosum with Cas9 Counterselection for Markerless Genome Engineering	19
Design–Build–Test of Synthetic Promoters for Inducible Gene Regulation in Alphaproteobacteria	19
Engineering a Novel Acetyl-CoA Pathway for Efficient Biosynthesis of Acetyl-CoA-Derived Compounds	19
Construction and Applications of Mammalian Cell-Based DNA-Encoded Peptide/Protein Libraries	19
Development of a Cell-Based Biosensor for Residual Detergent Detection in Decellularized Scaffolds	19
A Computational Framework for Identifying Promoter Sequences in Nonmodel Organisms Using RNA-seq Data Sets	19
Validation of Cell-Free Protein Synthesis Aboard the International Space Station	19
A Cell-Free Biosensor for Assessment of Hyperhomocysteinemia	18
CUBIC: A Versatile Cumate-Based Inducible CRISPRi System in Streptomyces	18
Cell-Free Biosensing Genetic Circuit Coupled with Ribozyme Cleavage Reaction for Rapid and Sensitive Detection of Small Molecules	18
Potential and Optimization of Mammalian Splice Riboswitches for the Regulation of Exon Skipping-Dependent Gene Expression and Isoform Switching within the ALOX5 Gene	18
Plasmid Crosstalk in Cell-Free Expression Systems	18
Context-Aware Technology Mapping in Genetic Design Automation	18
Using Protein Design and Directed Evolution to Monomerize a Bright Near-Infrared Fluorescent Protein	18
Predicting and Interpreting Protein Developability Via Transfer of Convolutional Sequence Representation	18
Streamlined Human Cell-Based Recombinase-Mediated Cassette Exchange Platform Enables Multigene Expression for the Production of Therapeutic Proteins	18
Using High-Throughput Experiments To Screen N-Glycosyltransferases with Altered Specificities	18
EngineeredSaccharomyces cerevisiaeas a Biosynthetic Platform of Nucleotide Sugars	18
A Photoresponsive Homing Endonuclease for Programmed DNA Cleavage	18
Cell-Free Expression of Soluble Leafhopper Proteins from Brochosomes	18
Improved Membrane Permeability via Hypervesiculation for In Situ Recovery of Lycopene in Escherichia coli	18
Comprehensive Parameter Space Mapping of Cell Cycle Dynamics under Network Perturbations	18
Preventing Production Escape Using an Engineered Glucose-Inducible Genetic Circuit	18
Experimental Data Connector (XDC): Integrating the Capture of Experimental Data and Metadata Using Standard Formats and Digital Repositories	18
A Hitchhiker’s Guide to Supplying Enzymatic Reducing Power into Synthetic Cells	18
minChemBio: Expanding Chemical Synthesis with Chemo-Enzymatic Pathways Using Minimal Transitions	18
Developing Biosensors for Specific Assessment of Trans-Translation in Pseudomonas aeruginosa	18
Tunable T7 Promoter Orthogonality on T7RNAP for cis-Aconitate Decarboxylase Evolution via Base Editor and Screening from Itaconic Acid Biosensor	18
ScEnSor Kit for Saccharomyces cerevisiae Engineering and Biosensor-Driven Investigation of the Intracellular Environment	18
Understanding Autologous Spliceostatin Transcriptional Regulation to Derive Parts for Heterologous Expression in a Burkholderia Bacterial Host	18
Synthetic Genetic Elements Enable Rapid Characterization of Inorganic Carbon Uptake Systems in Cupriavidus necator H16	17
Interrogating the Function of Bicistronic Translational Control Elements to Improve Consistency of Gene Expression	17
Engineering Cyanobacterial Cell Factories for Photosynthetic Production of Fructose	17
Degron-Controlled Protein Degradation in Escherichia coli: New Approaches and Parameters	17
Morphological and Metabolic Engineering of Yarrowia lipolytica to Increase β-Carotene Production	17
Synthetic Biology Design as a Paradigm Shift toward Manufacturing Affordable Adeno-Associated Virus Gene Therapies	17
In Vitro Transcription–Translation in an Artificial Biomolecular Condensate	17
Development of Solid-State Storage for Cell-Free Expression Systems	17
Progress in Gene Editing and Metabolic Regulation of Saccharomyces cerevisiae with CRISPR/Cas9 Tools	17
Building an RNA-Based Toggle Switch Using Inhibitory RNA Aptamers	17
Adaptive Optimization Boosted the Production of Moenomycin A in the Microbial Chassis Streptomyces albus J1074	17
Efficient Mutasynthesis of “Non-Natural” Antitubercular Ilamycins with Low Cytotoxicity	17
Engineered Mutants of a Marine Photosynthetic Purple Nonsulfur Bacterium with Increased Volumetric Productivity of Polyhydroxyalkanoate Bioplastics	17
Increased Cordycepin Production in Yarrowia lipolytica Using Combinatorial Metabolic Engineering Strategies	17
Establishing CRISPRi for Programmable Gene Repression and Genome Evolution in Cupriavidus necator	17
Thylakoid Targeting Improves Stability of a Cytochrome P450 in the Cyanobacterium Synechocystis sp. PCC 6803	17
Simplified Construction of Engineered Bacillus subtilis Host for Improved Expression of Proteins Harboring Noncanonical Amino Acids	17
Repurposing Peptide Nanomaterials as Synthetic Biomolecular Condensates in Bacteria	17
Let There Be Light: Genome Reduction Enables Bacillus subtilis to Produce Disulfide-Bonded Gaussia Luciferase	17
Modulating Liposome Surface Charge for Maximized ATP Regeneration in Synthetic Nanovesicles	17
Engineering Tropism of Pseudomonas putida toward Target Surfaces through Ectopic Display of Recombinant Nanobodies	17
De Novo Biosynthesis of the Anticancer Compound Euphol in Saccharomyces cerevisiae	17
De Novo Biosynthesis of a Polyene-Type Ginsenoside Precursor Dammaradienol in Saccharomyces cerevisiae	16
Large Flux Supply of NAD(H) under Aerobic Conditions by Candida glycerinogenes	16
Catalytically Active Inclusion Bodies─Benchmarking and Application in Flow Chemistry	16
Dual Antibiotic-Free Plasmid Systems Enable High-Efficiency l-Fucose Biosynthesis	16
Nano-Regulation of Gene Expression in Chlamydomonas reinhardtii: Harnessing AuNPs for Remotely Switchable Lipid Biosynthesis via Antisense Oligonucleotides	16
A System for the Evolution of Protein–Protein Interaction Inducers	16
Deep Mutational Scanning of an Oxygen-Independent Fluorescent Protein CreiLOV for Comprehensive Profiling of Mutational and Epistatic Effects	16
Systematic In Vivo Characterization of Fluorescent Protein Maturation in Budding Yeast	16
Engineered l-Lactate Responding Promoter System Operating in Glucose-Rich and Anoxic Environments	16
Investigating and Engineering an 1,2-Propanediol-Responsive Transcription Factor-Based Biosensor	16
Bioengineering Self-Organizing Signaling Centers to Control Embryoid Body Pattern Elaboration	16
Synthetic Gene Circuits Combining CRISPR Interference and CRISPR Activation in E. coli: Importance of Equal Guide RNA Binding Affinities to Avoid Context-Dependent Effects	16
Maximizing Heterologous Expression of Engineered Type I Polyketide Synthases: Investigating Codon Optimization Strategies	16
Engineering Tissue-Scale Properties with Synthetic Cells: Forging One from Many	16
Modular Pathway Compartmentalization for Agroclavine Overproduction in Saccharomyces cerevisiae	16
Systems Analysis of Highly Multiplexed CRISPR-Base Editing in Streptomycetes	16
Characterization and Application of a Stationary Phase Promoter Library from Serratia marcescens	16
CRISPR-Cas12a-Based Detection of SARS-CoV-2 Harboring the E484K Mutation	16
Sustainable Synthesis of Novel Green-Based Nanoparticles for Therapeutic Interventions and Environmental Remediation	16
CRISPR-Cas13 System as a Promising and Versatile Tool for Cancer Diagnosis, Therapy, and Research	16
Establishment of Green- and Red-Fluorescent Reporter Proteins Based on the Fluorescence-Activating and Absorption-Shifting Tag for Use in Acetogenic and Solventogenic Anaerobes	16
Coupling High-Throughput and Targeted Screening for Identification of Nonobvious Metabolic Engineering Targets	16
“Multiagent” Screening Improves Directed Enzyme Evolution by Identifying Epistatic Mutations	16
Recent Advances and Multiple Strategies of Monoterpenoid Overproduction in Saccharomyces cerevisiae and Yarrowia lipolytica	16
Yeast Metabolic Engineering for Biosynthesis of Caffeic Acid-Derived Phenethyl Ester and Phenethyl Amide	16
Engineered Attenuated Salmonella typhimurium Expressing Neoantigen Has Anticancer Effects	16
Perspectives on Genetically Engineered Microorganisms and Their Regulation in the United States	16
Nucleic Transformer: Classifying DNA Sequences with Self-Attention and Convolutions	16
Design of a Genetically Programmable and Customizable Protein Scaffolding System for the Hierarchical Assembly of Robust, Functional Macroscale Materials	16
A Titratable Cell Lysis-on-Demand System for Droplet-Compartmentalized Ultrahigh-Throughput Screening in Functional Metagenomics and Directed Evolution	15
Enhancement of β-Caryophyllene Biosynthesis in Saccharomyces cerevisiae via Synergistic Evolution of β-Caryophyllene Synthase and Engineering the Chassis	15
A Minispidroin Guides the Molecular Design for Cellular Condensation Mechanisms in S. cerevisiae	15
Issue Editorial Masthead	15
Robust Photocontrol of Elastin-like Polypeptide Phase Transition with a Genetically Encoded Arylazopyrazole	15
Non-native Pathway Engineering with CRISPRi for Carbon Dioxide Assimilation and Valued 5-Aminolevulinic Acid Synthesis in Escherichia coli Nissle	15
Efficient Enhancement of Extracellular Electron Transfer in Shewanella oneidensis MR-1 via CRISPR-Mediated Transposase Technology	15
Constructing Marine Bacterial Metabolic Chassis for Potential Biorefinery of Red Algal Biomass and Agaropectin Wastes	15
Bayesian Regression Facilitates Quantitative Modeling of Cell Metabolism	15