ACS Synthetic Biology

Article	Citations
Preventing Production Escape Using an Engineered Glucose-Inducible Genetic Circuit	145
The Japan-UK Synthetic Biology Conference, Spring 2025: Strengthening Global Links to Engineer Biology	142
Issue Publication Information	102
Stem Loop Mediated Transgene Modulation in Human T Cells	100
Construction of an Engineered Escherichia coli with Efficient Chemotactic and Metabolizing Ability toward Tetrathionate	85
A Photoresponsive Homing Endonuclease for Programmed DNA Cleavage	70
Issue Publication Information	68
Cell-Free Transcription and Translation Tether Expressed Peptides to Their Encoding Circular DNA	65
Targeting Transcriptional and Translational Hindrances in a Modular T7RNAP Expression System in Engineered Pseudomonas putida	62
Cell-Free Expression of Soluble Leafhopper Proteins from Brochosomes	61
DisGUVery: A Versatile Open-Source Software for High-Throughput Image Analysis of Giant Unilamellar Vesicles	59
Plasmid Copy Number Engineering Accelerates Fungal Polyketide Discovery upon Unnatural Polyketide Biosynthesis	59
Predicting and Interpreting Protein Developability Via Transfer of Convolutional Sequence Representation	58
Cell Free Bacteriophage Synthesis from Engineered Strains Improves Yield	58
Combining Chemical Catalysis with Enzymatic Steps for the Synthesis of the Artemisinin Precursor Dihydroartemisinic Acid	57
Protease-Responsive Toolkit for Conditional Targeted Protein Degradation	56
Energy Aware Technology Mapping of Genetic Logic Circuits	53
Development of a Recombineering System for the Acetogen Eubacterium limosum with Cas9 Counterselection for Markerless Genome Engineering	51
Desiccated Cyanobacteria Serve As Efficient Plasmid DNA Carriers in Space Flight	50
Directed Evolution of a SelB Variant that Does Not Require a Selenocysteine Insertion Sequence Element for Function	49
Preclinical Evaluation of Synthetic Biology-Driven Engineered Escherichia coli Nissle 1917 as a Living Therapeutic for Sustained L-DOPA Delivery	47
Engineering a Novel Acetyl-CoA Pathway for Efficient Biosynthesis of Acetyl-CoA-Derived Compounds	47
Highly Accurate Sequence- and Position-Independent Error Profiling of DNA Synthesis and Sequencing	46
Efficient Mutasynthesis of “Non-Natural” Antitubercular Ilamycins with Low Cytotoxicity	46
An Autocatalytic Peptide Cyclase Improves Fidelity and Yield of Circular Peptides In Vivo and In Vitro	46

Metabolic Engineering of Microbial Chassis Cells for d-Pantothenate Production: A Review	46
Mobile Translation Systems Generate Genomically Engineered Escherichia coli Cells with Improved Growth Phenotypes	45
A Logically Reversible Double Feynman Gate with Molecular Engineered Bacteria Arranged in an Artificial Neural Network-Type Architecture	44
IWBDA 2022: Toward a Modular Future of Synthetic Biology Driven by Bio-Design Automation	44
In VivoSampling of Intracellular Heterogeneity ofPseudomonas putidaEnables Multiobjective Optimization of Genetic Devices	43
Nano-Regulation of Gene Expression in Chlamydomonas reinhardtii: Harnessing AuNPs for Remotely Switchable Lipid Biosynthesis via Antisense Oligonucleotides	43
Efficient Genome Editing in Most Staphylococcus aureus by Using the Restriction-Modification System Silent CRISPR-Cas9 Toolkit	43
Control of λ Lysogenic Escherichia coli Cells by Synthetic λ Phage Carrying cIantisense	42
Simplified Construction of Engineered Bacillus subtilis Host for Improved Expression of Proteins Harboring Noncanonical Amino Acids	42
Issue Publication Information	41
Bacteria Engineered to Produce Serotonin Modulate Host Intestinal Physiology	41
Using Domain Insertion to Create Sulfite Reductases That Present Chemical-Dependent Activities	40
Actomyosin-Driven Division of a Synthetic Cell	39
Cell-Free Protein Synthesis as a Method to Rapidly Screen Machine Learning-Generated Protease Variants	39
Hybrid Methylotrophic Pathway in Serratia marcescens for Sustainable Terpenoid Biosynthesis	38
Improved Cell-Free Transcription–Translation Reactions in Microfluidic Chemostats Augmented with Hydrogel Membranes for Continuous Small Molecule Dialysis	38
Recent Advances in the Biological Synthesis of l-Tyrosine Derivatives by Genetically Engineered Microbes	38
Strategies for Improved Docosahexaenoic Acid Synthesis in Microalgae	38
Competitive Metabolism of Terephthalic Acid by a Consortium of Native and Engineered Bacteria	37
Maximizing Heterologous Expression of Engineered Type I Polyketide Synthases: Investigating Codon Optimization Strategies	37
SARGE: Serine Integrase-Assisted Rapid Genome Integration through Cassette Exchange in Escherichia coli	37
Metabolic Engineering of Clostridium tyrobutyricum for High-Yield n-Butanol Production by Increasing Intracellular Reducing Equivalent with NADPH-Dependent 3-Hydroxybutyryl-CoA Dehydroge	37
Lipid Modification and Membrane Localization of Proteins in Cell-Free System	36
An Optimized Genotyping Workflow for Identifying Highly SCRaMbLEd Synthetic Yeasts	36
Synthetic Biology Design as a Paradigm Shift toward Manufacturing Affordable Adeno-Associated Virus Gene Therapies	36
LevSeq: Rapid Generation of Sequence-Function Data for Directed Evolution and Machine Learning	36
Engineering Tissue-Scale Properties with Synthetic Cells: Forging One from Many	36
Engineering Cyanobacterial Cell Factories for Photosynthetic Production of Fructose	36
Issue Publication Information	35
Rational Protein Engineering of Branched-Chain α-Keto Acid Decarboxylase for Enhanced 3-Hydroxypropionic Acid Production in Escherichia coli	35
CRISPR Diagnostics for Quantification and Rapid Diagnosis of Myotonic Dystrophy Type 1 Repeat Expansion Disorders	35
A Constrained Degron Tag Engineered to Enhance Auxin-Inducible Protein Degradation	35
Combinatorial Nonribosomal Peptide Synthetase Libraries Using the SEAM-Combi-OGAB Method	34
An Arrayed CRISPR Screen Identifies Knockout Combinations Improving Antibody Productivity in HEK293 Cells	34
Optogenetic Control of Phosphate-Responsive Genes Using Single-Component Fusion Proteins in Saccharomyces cerevisiae	34
Directed Design of Anti-Idiotypic Nanobody with Antibacterial Activity against Foodborne Staphylococcus aureus and Its Innovative Application in Green Immunoassay	34
Genetic Engineering of Bacteriophage K1F with Human Epidermal Growth Factor to Enhance Killing of Intracellular E. coli K1	34
RNA Aptamer-Mediated Gene Activation Systems for Inducible Transgene Expression in Animal Cells	34
Directing Uphill Strand Displacement with an Engineered Superhelicase	34
Engineered Acinetobacter baylyi ADP1-ISx Cells Are Sensitive DNA Biosensors for Antibiotic Resistance Genes and a Fungal Pathogen of Bats	33
Extracellular Cues Govern Shape and Cytoskeletal Organization in Giant Unilamellar Lipid Vesicles	33
Cargo-Directed Assembly of Nonviral Nucleocapsid with Controlled Size	33
Harnessing the Endogenous Type I–F CRISPR/Cas System for Efficient Genome Engineering and Gene Repression in Pseudomonas chlororaphis LX24	33
Characterization of Integrase and Excisionase Activity in a Cell-Free Protein Expression System Using a Modeling and Analysis Pipeline	32
Genome-Scale CRISPRi Screening Identifies Gene Targets for Enhanced Octanoic Acid Tolerance and Production in Escherichia coli	32
An Explainable Deep Learning Framework Integrating DNA Sequence and Transcription Initiation Signals for Gene Expression Prediction	31
Pathway Refactoring for Efficient 7-Dehydrocholesterol Production in Saccharomyces cerevisiae	31
Toward a Circular Bioeconomy: Designing Microbes and Polymers for Biodegradation	31
Spatiotemporal Gene Expression by a Genetic Circuit for Chemical Production in Escherichia coli	30
Clonal Amplification-Enhanced Gene Expression in Synthetic Vesicles	30

Reducing Cellular Autolysis of Bacillus subtilis to Improve Keratinase Production	30
Tunable Transcription-Level CRISPR Interference in Vibrio natriegens Using Engineered Single Guide RNAs	30
RAGATH-Associated DNA Nuclease Assisted DNA Insertion in Corynebacterium glutamicum	30
Heterologous Rieske Nonheme Iron Monooxygenases Enable Efficient Microbial Conversion of Lignin Guaiacol to Adipic Acid	29
Zymo-Parts: A Golden Gate Modular Cloning Toolbox for Heterologous Gene Expression in Zymomonas mobilis	28
Machine Learning for Microbial Cell Factories: Pathway Design, Enzyme Engineering, and Metabolic Regulation	28
Flavin-Decorated Proteins and Bacteria Enable Photoenzymatic Amine Valorisation	28
LysePred: A Multiscale Convolutional Neural Network for Predicting Hemolytic Activity of Antimicrobial Peptides	28
AutoBioTech─A Versatile Biofoundry for Automated Strain Engineering	28
High-Efficiency Multiplexed Cytosine Base Editors for Natural Product Synthesis in Yarrowia lipolytica	28
Hybrid Cas12a Variants with Relaxed PAM Requirements Expand Genome Editing Compatibility	27
CRISPR-Based Regulation for High-Throughput Screening	27
Fine-Regulating the Carbon Flux of l-Isoleucine Producing Corynebacterium glutamicum WM001 for Efficient l-Threonine Production	27
Combinatorial Tuning of 5′UTR and N-Terminal Coding Sequences for Enhanced Recombinant Protein Expression in Corynebacterium glutamicum	27
Vacuum and Sonication Treatment Enable Efficient Transient Gene Expression in Various Monocot and Eudicot Plant Seedlings	27
Development of a Type I-E CRISPR-Based Programmable Repression System for Fine-Tuning Metabolic Flux toward D-Pantothenic Acid in Bacillus subtilis	27
De Novo Production of the Bioactive Phenylpropanoid Artepillin C Using Membrane-Bound Prenyltransferase in Komagataella phaffii	26
Issue Publication Information	26
Induced Neural Progenitor Specification from Human Pluripotent Stem Cells by a Refined Synthetic Notch Platform	26
A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of Saccharomyces cerevisiae	26
Identifying Antisense Oligonucleotides to Disrupt Small RNA Regulated Antibiotic Resistance via a Cell-Free Transcription–Translation Platform	26
Systematic Engineering of Escherichia coli for Efficient Production of Nicotinamide Mononucleotide From Nicotinamide	26
Modular Metabolic Engineering and Synthetic Coculture Strategies for the Production of Aromatic Compounds in Yeast	26
Enzymatic Supercoiling of Bacterial Chromosomes Facilitates Genome Manipulation	25
Issue Publication Information	25
Discovery of Latent Cannabichromene Cyclase Activity in Marine Bacterial Flavoenzymes	25
Iron-Tannin Coating Reduces Clearance and Increases Tumor Colonization of Systemically Delivered Bacteria	25
Issue Publication Information	25
Efficient Bacterial Genome Engineering throughout the Central Dogma Using the Dual-Selection Marker tetAOPT	24
Issue Editorial Masthead	24
Precision Transcriptome Editing	24
Amplification-Free COVID-19 Detection by Digital Droplet REVEALR	24
Quantitative Dissection of Agrobacterium Virulence to Generate a Synthetic Ti Plasmid	24
A High-Throughput Colocalization Pipeline for Quantification of Mitochondrial Targeting across Different Protein Types	24
Implementation of a Novel Optogenetic Tool in Mammalian Cells Based on a Split T7 RNA Polymerase	24
HinZip: Combining Hin Recombinase and FosW to Mimic HD-Zip Plant Proteins	24
Elucidating the Metabolic Landscape of p -Coumaric Acid to Deepen Biological Implication and Enhance Biomanufacturing	24
One-Pot De Novo Synthesis of [4Fe-4S] Proteins Using a Recombinant SUF System under Aerobic Conditions	24
Exploration of the Native Sucrose Operon Enables the Development of an Inducible T7 Expression System in Paenibacillus polymyxa	24
pUdOs: Concise Plasmids for Bacterial and Mammalian Cells	24
Engineering and Optimization of the Salidroside Production in Tobacco Cells by Reconstructed Biosynthetic Genes from Rhodiola rosea	24
An Integrative Strategy Enhancing Nanobody Thermostability via CDR Grafting, In Silico Mutagenesis Screening, and Multiplex Evaluation	23
Precision Genome Engineering in Streptococcus suis Based on a Broad-Host-Range Vector and CRISPR-Cas9 Technology	23
Optically Controlled CRISPR-Cas9 and Cre Recombinase for Spatiotemporal Gene Editing: A Review	23
Light-Induced Nanobody-Mediated Targeted Protein Degradation for Metabolic Flux Control	23
Expanding the Cell-Free Reporter Protein Toolbox by Employing a Split mNeonGreen System to Reduce Protein Synthesis Workload	23
Modular and Flexible Molecular Device for Simultaneous Cytosine and Adenine Base Editing at Random Genomic Loci in Filamentous Fungi	23
Functional Modification of Cyanobacterial Phycobiliprotein and Phycobilisomes through Bilin Metabolism Control	23
Harnessing Escherichia coli’s Native Machinery for Detection of Vitamin C (Ascorbate) Deficiency	23
Multifunctional Roles and Microbial Production Bottlenecks of Ergothioneine	23
Highly Parallelized Construction of DNA from Low-Cost Oligonucleotide Mixtures Using Data-Optimized Assembly Design and Golden Gate	23
Screening, Characterization, and Engineering of Universal Promoters for Bifidobacterium breve	23
Hyperstable Synthetic Mini-Proteins as Effective Ligand Scaffolds	22
Advancement of Research Progress on Synthesis Mechanism of Cannabidiol (CBD)	22
ATP-Binding Cassette Exporter PDR11-Mediated Terpenoid Secretion in Engineered Yeast	22
Chloroplast Cell-Free Systems from Different Plant Species as a Rapid Prototyping Platform	22
Special Issue on Artificial Intelligence for Synthetic Biology	22
Automated Construction of a Yeast-Based Multigene Library via Homologous Recombination in a Biofoundry Workflow	22
DeCOIL: Optimization of Degenerate Codon Libraries for Machine Learning-Assisted Protein Engineering	22
Nucleotide-Level Chemical Reaction Network Modeling Enables Quantitative Prediction of Reconstituted Cell-Free Expression Systems	22
Reconstruction of a Genome-Scale Metabolic Model of Scenedesmus obliquus and Its Application for Lipid Production under Three Trophic Modes	22
Coordinating Calvin Cycle and Glycolysis in Escherichia coli	22
Metabolic Engineering of Escherichia coli for Self-Induced Production of l-Isoleucine	22
Engineering Escherichia coli for Perillyl Alcohol Production with Reduced Endogenous Dehydrogenation	22
RNA-Based Sensor Systems for Affordable Diagnostics in the Age of Pandemics	22
Pseudotyping Bacteriophage P2 Tail Fibers to Extend the Host Range for Biomedical Applications	22
A Layered Genetic Design Enables the Yeast Galactose Regulon to Respond to Cyanamide	21
Engineering a Synthetic Escherichia coli Coculture for Compartmentalized de novo Biosynthesis of Isobutyl Butyrate from Mixed Sugars	21
Issue Editorial Masthead	21
Evaluating Apoptotic Gene Efficiency for CHO Culture Performance Using Targeted Integration	21
A Ubiquitous Chromatin Opening Element and DNA Demethylation Facilitate Doxycycline-Controlled Expression during Differentiation and in Transgenic Mice	21
Heterologous Expression and CRISPR/Cas9-Assisted Manipulation of the Hybrid Gene Cluster Specifying the Biosynthesis of Meroterpenoids and Phenazines	21
Issue Editorial Masthead	21
Broad-Host-Range Synthetic Biology: Rethinking Microbial Chassis as a Design Variable	21
Endotoxin-Free Outer Membrane Vesicles for Safe and Modular Anticancer Immunotherapy	21
Engineering Microbial Consortium Biohybrid System to Efficiently Produce Electricity from Lignocellulose Biomass	21
An In Vitro Hybrid Biocatalytic System Enabled by a Combination of Surface-Displayed, Purified, and Cell-Free Expressed Enzymes	21
DNA Origami Nanostructure Detection and Yield Estimation Using Deep Learning	21
Correction to “Toward a Circular Bioeconomy: Designing Microbes and Polymers for Biodegradation”	21
Issue Editorial Masthead	21

The Translational Coupling of Daidzein Reductase and Dihydrodaidzein Racemase Genes Improves the Production of Equol and Its Analogous Derivatives in Engineered Lactic Acid Bacteria	21
Engineering Temperature-Powered Synthetic Multilayer Molecular Bioswitch for High-Level Pyruvate Derivative Production in Escherichia coli	20
HUHgle: An Interactive Substrate Design Tool for Covalent Protein-ssDNA Labeling Using HUH-Tags	20
Synthetic Homoserine Lactone Sensors for Gram-Positive Bacillus subtilis Using LuxR-Type Regulators	20
Prompt-Only Gene-Circuit Modeling with a Large Language Model Simulates the synNotch Spheroids	20
Spatially Selective Electrochemical Cleavage of a Polymerase-Nucleotide Conjugate	20
Genome-Wide CRISPR/Cas9 Screening Unveils a Novel Target ATF7IP–SETDB1 Complex for Enhancing Difficult-to-Express Protein Production	20
Design of Four Small-Molecule-Inducible Systems in the Yeast Chromosome, Applied to Optimize Terpene Biosynthesis	20
Programmable DNA-Based Molecular Reservoir Biocomputing Network Circuits with Emerging Biomemristors for Solving Complex Nonlinear Problems	20
Nitroxysomes as a Potential Solution for Engineering Biological Nitrogen Fixation	20
Engineering Mixotrophy in the Chemolithoautotrophic Cupriavidus necator through Hydrogenase Induction	20
Recombinant Protein Expression Chassis Library of Vibrio natriegens by Fine-Tuning the Expression of T7 RNA Polymerase	20
Inducible CRISPR/Cas9 Allows for Multiplexed and Rapidly Segregated Single-Target Genome Editing in Synechocystis Sp. PCC 6803	20
Imaging Method Using CRISPR/dCas9 and Engineered gRNA Scaffolds Can Perturb Replication Timing at the HSPA1 Locus	20
Engineered Microbial Production of Sesquiterpenoids: Recent Advances and Prospects	20
Metabolic Engineering of Saccharomyces cerevisiae for High-Level Production of l-Pipecolic Acid from Glucose	20
Side-by-Side Systematic Characterization of Novel FPs in Budding Yeast	20
Biomolecular Condensate-Based Artificial Organelle for Driving Compartmentalized Flux Control	20
Engineered Stop and Go T7 RNA Polymerases	20
Pooled CRISPR Interference Screening Identifies Crucial Transcription Factors in Gas-Fermenting Clostridium ljungdahlii	20
Plant Synthetic Biology Takes Off: Bryophytes Onboard with New Tools, Systems, and Opportunities	20
Tailoring Bacterial Cellulose through the CRISPR/Cas9-Mediated Gene Editing Tool in Komagataeibacter xylinus	20
Conditional Control of Universal CAR T Cells by Cleavable OFF-Switch Adaptors	20
Fast and Simplified CRISPR–Cas13d Assay for Sensitive Detection of Porcine Deltacoronavirus	20
Marine Biofilm Engineered to Produce Current in Response to Small Molecules	20
Tunable and Orthogonal ECF and Anti-σ Threshold Gates for Temporal Control of Heterologous Gene Expression	19
Robust Synthetic Biology Toolkit to Advance Carboxysome Study and Redesign	19
Transcriptomics-Driven Engineering of Yarrowia lipolytica for Enhanced Fatty Acid Biosynthesis	19
Functional Deimmunization of Botulinum Neurotoxin Protease Domain via Computationally Driven Library Design and Ultrahigh-Throughput Screening	19
Biosynthesis of the Functional Component Spermidine from Bacillus amyloliquefaciens by Iterative Integration Expression	19
Biosynthesis of Antimicrobial Ornithine-Containing Lacticin 481 Analogues by Use of a Combinatorial Biosynthetic Pathway in Escherichia coli	19
An Engineered Variant of E. coli Nissle 1917 with Enhanced Transformation Efficiency and Robustness	19
Point-of-Care Peptide Hormone Production Enabled by Cell-Free Protein Synthesis	19
Low Resource Integrated Platform for Production and Analysis of Capped mRNA	19
Unraveling the Constrained Cell Growth in Engineered Living Materials	19
Breaking the Stability-Activity-Selectivity Trilemma in Unspecific Peroxygenase through Computation-Based Cross-Regional Combinatorial Mutagenesis	19
Metabolic Engineering of Candida glycerinogenes for Sustainable Production of Geraniol	19
Comparison of Kill Switch Toxins in Plant-Beneficial Pseudomonas fluorescens Reveals Drivers of Lethality, Stability, and Escape	19
A Novel Sesquiterpene Synthase from Neophaeococcomyces mojaviensis for α-Santalene Production	19
One-Step Multi-fragment Assembly and Targeted Genomic Integration in Corynebacterium glutamicum	19
Genetic Code Expansion in Pseudomonas putida KT2440	18
Metabolic Engineering of Methanotrophic Bacteria for De Novo Production of Taxadiene from Methane	18
Machine Learning in Microbiome Research and Engineering	18
Statistical Analysis and Tokenization of Epitopes to Construct Artificial Neoepitope Libraries	18
Robust Inducible Gene Expression in Intracellular Listeria monocytogenes In Vivo	18
Improved l -Cysteine Production in Corynebacterium glutamicum through Metabolic Engineering and High-T	18
Logic Circuits Based on 2A Peptide Sequences in the Yeast Saccharomyces cerevisiae	18
GOLDBAR: A Framework for Combinatorial Biological Design	18
Modular Design of a Copper Ion Biosensor Based on the MAPK Signaling Pathway in Saccharomyces cerevisiae	18
Issue Publication Information	18
Correction to “Hyperaccurate Ribosomes for Improved Genetic Code Reprogramming”	18
Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and Identification of Potential Bacterial Chassis for Studying Plant–Microbe Interactions	18
Antibiotics-Free Steady Bioproduction of Valuable Chemicals from Organic Wastes by Engineered Vibrio natriegens through Targeted Gene Integration	18
The Potential of Artificial Cells Functioning under In Situ Deep-Sea Conditions	18
Engineering a Yeast Cell Factory to Sustainably Biosynthesize Parthenolide	18
Conditionally Activated Cross-Linked crRNAs for CRISPR/Cas12a Based Nucleic Acid Detection	18
A Simple and Effective Strategy for the Development of Robust Promoter-Centric Gene Expression Tools	18
Synthetic Biology of Natural Products Engineering: Recent Advances Across the Discover–Design–Build–Test–Learn Cycle	18
A Decade of SBOL Visual: Growing Adoption of a Diagram Standard for Engineering Biology	18
Synthetic Platforms for Characterizing and Targeting of SARS-CoV-2 Genome Capping Enzymes	18
Efficient Autoinducible Expression of Recombinant Proteins via the DegSU Quorum Sensing System in a Robust Bacillus subtilis	18
Introduction to Engineering Biology: A Conceptual Framework for Teaching Synthetic Biology	18
GroovDB: A Database of Ligand-Inducible Transcription Factors	17
Tandem-sgRNA Provides an Effective Reverse Genetic Approach for Suppression of Streptomyces Biosynthetic Gene Clusters and Secondary Metabolism	17
Synthetic Ecosystems: From the Test Tube to the Biosphere	17
Sustainable Production of Bio-Based Geraniol: Heterologous Expression of Early Terpenoid Pathway Enzymes in Chlamydomonas reinhardtii	17
One-Pot Biocatalytic Route from Alkanes to α,ω-Diamines by Whole-Cell Consortia of Engineered Yarrowia lipolytica and Escherichia coli	17
Cross-Strain Transferability of CRISPRi Systems and Design Rules from Laboratory to Clinical Escherichia coli Strains	17
Comparative Evaluation of Engineered Bacteria and Yeast for Oral Delivery of CRISPR/Cas9 Systems in Colon Cancer Therapy	17
Organellar Glue: A Molecular Tool to Artificially Control Chloroplast–Chloroplast Interactions	17
A Single-Component Optogenetic Gal4-UAS System Allows Stringent Control of Gene Expression in Zebrafish and Drosophila	17
Combinatorial Engineering of Escherichia coli for Enhancing 3-Fucosyllactose Production	17
Rational Engineering of Escherichia coli Nissle 1917 as Live Biotherapeutic to Degrade Uremic Toxin Precursors	17
Engineering Yarrowia lipolytica to Produce l-Malic Acid from Glycerol	17
De Novo Synthesis of Tyrosol and Hydroxytyrosol through Temperature-Inducible Systems and Metabolic Engineering	17
M o SA I C: Codon Harmonization of Monte Carlo-Based Simulated Annealing for Linked Codons in Hete	17
Reliable Genomic Integration Sites in Pseudomonas putida Identified by Two-Dimensional Transcriptome Analysis	17
Simulations Reveal High Efficiency and Confinement of a Population Suppression CRISPR Toxin-Antidote Gene Drive	17
High-Efficiency Production of N -Acetyllactosamine (LacNAc) Using a Cell-Coupled Biocatalytic Strategy with Engineered Escherichia	17
Investigating the Specificity of the Dehydration and Cyclization Reactions in Engineered Lanthipeptides by Synechococcal SyncM	17
Microbial Synthesis of Nucleosides: Advances and Prospects	17
The iGEM Grand Jamboree and the Serious Fun of Engineering Biology	17
Identifying Conditions for Protein Synthesis Inside Giant Vesicles Using Microfluidics toward Standardized Artificial Cell Production	17
Cold Exposure and Oral Delivery of GLP-1R Agonists by an Engineered Probiotic Yeast Strain Have Antiobesity Effects in Mice	17
Cell-Free Expression System Derived from a Near-Minimal Synthetic Bacterium	17
Biotin-Independent Saccharomyces cerevisiae with Enhanced Growth: Engineering an Acetyl-CoA Carboxylase Bypass	17
Design of a Labile RNase A Using Protein Language Models	16
Issue Editorial Masthead	16
Engineering an Escherichia coli Autolytic System for Overexpression of a Collagen-Mimetic Peptide with Enhanced Hemostatic Activity	16
Development and Application of an ACQUIRE Method for Direct Cloning of Superlarge Biosynthetic Gene Cluster	16
Development of a Transcriptional Biosensor for Hydrogen Sulfide That Functions under Aerobic and Anaerobic Conditions	16
Optimization of Campesterol-Producing Yeast Strains as a Feasible Platform for the Functional Reconstitution of Plant Membrane-Bound Enzymes	16
Systematic Evaluation of Vector Sequence Elements in Isogenic CHO Cells for Improved Antibody Production	16
Bacterial Living Therapeutics with Engineered Protein Secretion Circuits to Eliminate Breast Cancer Cells	16
Engineering Clostridium Tyrobutyricum for High Butanol Production through Induction Expression of Exogenous NADPH-Dependent HBD	16