ACS Synthetic Biology

Article	Citations
Directed Evolution of a SelB Variant that Does Not Require a Selenocysteine Insertion Sequence Element for Function	107
Discovering Content through Text Mining for a Synthetic Biology Knowledge System	91
Synthetic Biology 2.0: The Dawn of a New Era	91
Mobile Translation Systems Generate Genomically Engineered Escherichia coli Cells with Improved Growth Phenotypes	75
Preventing Production Escape Using an Engineered Glucose-Inducible Genetic Circuit	74
Simplified Construction of Engineered Bacillus subtilis Host for Improved Expression of Proteins Harboring Noncanonical Amino Acids	72
The Japan-UK Synthetic Biology Conference, Spring 2025: Strengthening Global Links to Engineer Biology	71
Nano-Regulation of Gene Expression in Chlamydomonas reinhardtii: Harnessing AuNPs for Remotely Switchable Lipid Biosynthesis via Antisense Oligonucleotides	70
Cell-Free Expression of Soluble Leafhopper Proteins from Brochosomes	65
Metabolic Engineering of Microbial Chassis Cells for d-Pantothenate Production: A Review	63
Recent Advances in the Biological Synthesis of l-Tyrosine Derivatives by Genetically Engineered Microbes	62
Highly Accurate Sequence- and Position-Independent Error Profiling of DNA Synthesis and Sequencing	58
Issue Publication Information	55
Control of λ Lysogenic Escherichia coli Cells by Synthetic λ Phage Carrying cIantisense	53
Plasmid Copy Number Engineering Accelerates Fungal Polyketide Discovery upon Unnatural Polyketide Biosynthesis	52
Optimal LentiCRISPR-Based System for Sequential CRISPR/Cas9 Screens	52
Strategies for Improved Docosahexaenoic Acid Synthesis in Microalgae	51
Stem Loop Mediated Transgene Modulation in Human T Cells	51
Cell-Free Protein Synthesis as a Method to Rapidly Screen Machine Learning-Generated Protease Variants	50
LOICA: Integrating Models with Data for Genetic Network Design Automation	50
Bacteria Engineered to Produce Serotonin Modulate Host Intestinal Physiology	49
Targeting Transcriptional and Translational Hindrances in a Modular T7RNAP Expression System in Engineered Pseudomonas putida	48
Engineering Cyanobacterial Cell Factories for Photosynthetic Production of Fructose	48
IWBDA 2022: Toward a Modular Future of Synthetic Biology Driven by Bio-Design Automation	46
Combining Chemical Catalysis with Enzymatic Steps for the Synthesis of the Artemisinin Precursor Dihydroartemisinic Acid	46

DisGUVery: A Versatile Open-Source Software for High-Throughput Image Analysis of Giant Unilamellar Vesicles	45
A Photoresponsive Homing Endonuclease for Programmed DNA Cleavage	44
Desiccated Cyanobacteria Serve As Efficient Plasmid DNA Carriers in Space Flight	44
Energy Aware Technology Mapping of Genetic Logic Circuits	44
Engineered Mutants of a Marine Photosynthetic Purple Nonsulfur Bacterium with Increased Volumetric Productivity of Polyhydroxyalkanoate Bioplastics	41
Efficient Genome Editing in Most Staphylococcus aureus by Using the Restriction-Modification System Silent CRISPR-Cas9 Toolkit	41
Efficient Mutasynthesis of “Non-Natural” Antitubercular Ilamycins with Low Cytotoxicity	41
Cell-Free Transcription and Translation Tether Expressed Peptides to Their Encoding Circular DNA	38
Issue Publication Information	38
In VivoSampling of Intracellular Heterogeneity ofPseudomonas putidaEnables Multiobjective Optimization of Genetic Devices	37
Actomyosin-Driven Division of a Synthetic Cell	37
Cell Free Bacteriophage Synthesis from Engineered Strains Improves Yield	37
LevSeq: Rapid Generation of Sequence-Function Data for Directed Evolution and Machine Learning	36
Maximizing Heterologous Expression of Engineered Type I Polyketide Synthases: Investigating Codon Optimization Strategies	36
An Autocatalytic Peptide Cyclase Improves Fidelity and Yield of Circular Peptides In Vivo and In Vitro	36
Lipid Modification and Membrane Localization of Proteins in Cell-Free System	36
Construction of an Engineered Escherichia coli with Efficient Chemotactic and Metabolizing Ability toward Tetrathionate	36
Predicting and Interpreting Protein Developability Via Transfer of Convolutional Sequence Representation	35
Protease-Responsive Toolkit for Conditional Targeted Protein Degradation	35
Metabolic Engineering of Clostridium tyrobutyricum for High-Yield n-Butanol Production by Increasing Intracellular Reducing Equivalent with NADPH-Dependent 3-Hydroxybutyryl-CoA Dehydroge	35
A Logically Reversible Double Feynman Gate with Molecular Engineered Bacteria Arranged in an Artificial Neural Network-Type Architecture	35
Engineering a Novel Acetyl-CoA Pathway for Efficient Biosynthesis of Acetyl-CoA-Derived Compounds	35
Synthetic Biology Design as a Paradigm Shift toward Manufacturing Affordable Adeno-Associated Virus Gene Therapies	35
Engineering Tissue-Scale Properties with Synthetic Cells: Forging One from Many	35
Parameterized Computational Framework for the Description and Design of Genetic Circuits of Morphogenesis Based on Contact-Dependent Signaling and Changes in Cell–Cell Adhesion	34
Efficient CRISPR/Cas12a-Based Genome-Editing Toolbox for Metabolic Engineering in Methanococcus maripaludis	34
Development of a Recombineering System for the Acetogen Eubacterium limosum with Cas9 Counterselection for Markerless Genome Engineering	34
Improved Cell-Free Transcription–Translation Reactions in Microfluidic Chemostats Augmented with Hydrogel Membranes for Continuous Small Molecule Dialysis	34
An Arrayed CRISPR Screen Identifies Knockout Combinations Improving Antibody Productivity in HEK293 Cells	33
Combinatorial Nonribosomal Peptide Synthetase Libraries Using the SEAM-Combi-OGAB Method	33
Hybrid Methylotrophic Pathway in Serratia marcescens for Sustainable Terpenoid Biosynthesis	33
Genetic Engineering of Bacteriophage K1F with Human Epidermal Growth Factor to Enhance Killing of Intracellular E. coli K1	32
Issue Editorial Masthead	32
Combinatorial Tuning of 5′UTR and N-Terminal Coding Sequences for Enhanced Recombinant Protein Expression in Corynebacterium glutamicum	32
Directed Design of Anti-Idiotypic Nanobody with Antibacterial Activity against Foodborne Staphylococcus aureus and Its Innovative Application in Green Immunoassay	32
Clonal Amplification-Enhanced Gene Expression in Synthetic Vesicles	31
De Novo Production of the Bioactive Phenylpropanoid Artepillin C Using Membrane-Bound Prenyltransferase in Komagataella phaffii	31
RAGATH-Associated DNA Nuclease Assisted DNA Insertion in Corynebacterium glutamicum	31
RNA Aptamer-Mediated Gene Activation Systems for Inducible Transgene Expression in Animal Cells	31
GPCR-FEX: A Fluoride-Based Selection System for Rapid GPCR Screening and Engineering	31
Extracellular Cues Govern Shape and Cytoskeletal Organization in Giant Unilamellar Lipid Vesicles	30
Engineered Acinetobacter baylyi ADP1-ISx Cells Are Sensitive DNA Biosensors for Antibiotic Resistance Genes and a Fungal Pathogen of Bats	30
Harnessing the Endogenous Type I–F CRISPR/Cas System for Efficient Genome Engineering and Gene Repression in Pseudomonas chlororaphis LX24	30
Induced Neural Progenitor Specification from Human Pluripotent Stem Cells by a Refined Synthetic Notch Platform	30
Development of a Type I-E CRISPR-Based Programmable Repression System for Fine-Tuning Metabolic Flux toward D-Pantothenic Acid in Bacillus subtilis	30
Systematic Engineering of Escherichia coli for Efficient Production of Nicotinamide Mononucleotide From Nicotinamide	29
High-Efficiency Multiplexed Cytosine Base Editors for Natural Product Synthesis in Yarrowia lipolytica	29
CRISPR Diagnostics for Quantification and Rapid Diagnosis of Myotonic Dystrophy Type 1 Repeat Expansion Disorders	29
Optogenetic Control of Phosphate-Responsive Genes Using Single-Component Fusion Proteins in Saccharomyces cerevisiae	29
Zymo-Parts: A Golden Gate Modular Cloning Toolbox for Heterologous Gene Expression in Zymomonas mobilis	29

Pathway Refactoring for Efficient 7-Dehydrocholesterol Production in Saccharomyces cerevisiae	29
Combating Infectious Diseases with Synthetic Biology	29
Identifying Antisense Oligonucleotides to Disrupt Small RNA Regulated Antibiotic Resistance via a Cell-Free Transcription–Translation Platform	29
Directing Uphill Strand Displacement with an Engineered Superhelicase	28
DNA Input Classification by a Riboregulator-Based Cell-Free Perceptron	28
AutoBioTech─A Versatile Biofoundry for Automated Strain Engineering	28
Characterization of Integrase and Excisionase Activity in a Cell-Free Protein Expression System Using a Modeling and Analysis Pipeline	28
Fine-Regulating the Carbon Flux of l-Isoleucine Producing Corynebacterium glutamicum WM001 for Efficient l-Threonine Production	28
Spatiotemporal Gene Expression by a Genetic Circuit for Chemical Production in Escherichia coli	27
An Optimized Genotyping Workflow for Identifying Highly SCRaMbLEd Synthetic Yeasts	27
A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of Saccharomyces cerevisiae	27
Toward a Circular Bioeconomy: Designing Microbes and Polymers for Biodegradation	27
CRISPR-Based Regulation for High-Throughput Screening	27
RoboMoClo: A Robotics-Assisted Modular Cloning Framework for Multiple Gene Assembly in Biofoundry	27
Precision Transcriptome Editing	26
Issue Publication Information	26
Hybrid Cas12a Variants with Relaxed PAM Requirements Expand Genome Editing Compatibility	26
Issue Publication Information	26
Reducing Cellular Autolysis of Bacillus subtilis to Improve Keratinase Production	26
Functional and Structural Roles of the Dimer Interface in the Activity and Stability of Clostridium butyricum 1,3-Propanediol Oxidoreductase	26
Issue Publication Information	26
Modular Metabolic Engineering and Synthetic Coculture Strategies for the Production of Aromatic Compounds in Yeast	26
Engineering a Synthetic Dopamine-Responsive Riboswitch for In Vitro Biosensing	26
HinZip: Combining Hin Recombinase and FosW to Mimic HD-Zip Plant Proteins	25
Special Issue on Artificial Intelligence for Synthetic Biology	25
Iron-Tannin Coating Reduces Clearance and Increases Tumor Colonization of Systemically Delivered Bacteria	25
DNA Origami Nanostructure Detection and Yield Estimation Using Deep Learning	25
Amplification-Free COVID-19 Detection by Digital Droplet REVEALR	25
Hyperstable Synthetic Mini-Proteins as Effective Ligand Scaffolds	25
A High-Throughput Colocalization Pipeline for Quantification of Mitochondrial Targeting across Different Protein Types	25
Engineering and Optimization of the Salidroside Production in Tobacco Cells by Reconstructed Biosynthetic Genes from Rhodiola rosea	25
Endotoxin-Free Outer Membrane Vesicles for Safe and Modular Anticancer Immunotherapy	25
Optically Controlled CRISPR-Cas9 and Cre Recombinase for Spatiotemporal Gene Editing: A Review	24
One-Pot De Novo Synthesis of [4Fe-4S] Proteins Using a Recombinant SUF System under Aerobic Conditions	24
DeCOIL: Optimization of Degenerate Codon Libraries for Machine Learning-Assisted Protein Engineering	24
Metabolic Engineering of Escherichia coli for Self-Induced Production of l-Isoleucine	24
Chloroplast Cell-Free Systems from Different Plant Species as a Rapid Prototyping Platform	24
Functional Modification of Cyanobacterial Phycobiliprotein and Phycobilisomes through Bilin Metabolism Control	24
Efficient Bacterial Genome Engineering throughout the Central Dogma Using the Dual-Selection Marker tetAOPT	24
Combinatorial PCR Method for Efficient, Selective Oligo Retrieval from Complex Oligo Pools	24
PASIV: A Pooled Approach-Based Workflow to Overcome Toxicity-Induced Design of Experiments Failures and Inefficiencies	24
Engineering a Synthetic Escherichia coli Coculture for Compartmentalized de novo Biosynthesis of Isobutyl Butyrate from Mixed Sugars	24
Reconstruction of a Genome-Scale Metabolic Model of Scenedesmus obliquus and Its Application for Lipid Production under Three Trophic Modes	24
Broad-Host-Range Synthetic Biology: Rethinking Microbial Chassis as a Design Variable	23
Implementation of a Novel Optogenetic Tool in Mammalian Cells Based on a Split T7 RNA Polymerase	23
Advancement of Research Progress on Synthesis Mechanism of Cannabidiol (CBD)	23
Pseudotyping Bacteriophage P2 Tail Fibers to Extend the Host Range for Biomedical Applications	23
Precision Genome Engineering in Streptococcus suis Based on a Broad-Host-Range Vector and CRISPR-Cas9 Technology	23
Engineering Escherichia coli for Perillyl Alcohol Production with Reduced Endogenous Dehydrogenation	23
Issue Editorial Masthead	23
An Integrative Strategy Enhancing Nanobody Thermostability via CDR Grafting, In Silico Mutagenesis Screening, and Multiplex Evaluation	23
Expanding the Cell-Free Reporter Protein Toolbox by Employing a Split mNeonGreen System to Reduce Protein Synthesis Workload	23
Automated Construction of a Yeast-Based Multigene Library via Homologous Recombination in a Biofoundry Workflow	23
pUdOs: Concise Plasmids for Bacterial and Mammalian Cells	23
Enzymatic Supercoiling of Bacterial Chromosomes Facilitates Genome Manipulation	23
Exploration of the Native Sucrose Operon Enables the Development of an Inducible T7 Expression System in Paenibacillus polymyxa	23
Modular and Flexible Molecular Device for Simultaneous Cytosine and Adenine Base Editing at Random Genomic Loci in Filamentous Fungi	23
Light-Induced Nanobody-Mediated Targeted Protein Degradation for Metabolic Flux Control	22
Highly Parallelized Construction of DNA from Low-Cost Oligonucleotide Mixtures Using Data-Optimized Assembly Design and Golden Gate	22
RNA-Based Sensor Systems for Affordable Diagnostics in the Age of Pandemics	22
A Layered Genetic Design Enables the Yeast Galactose Regulon to Respond to Cyanamide	22
Harnessing Escherichia coli’s Native Machinery for Detection of Vitamin C (Ascorbate) Deficiency	22
ATP-Binding Cassette Exporter PDR11-Mediated Terpenoid Secretion in Engineered Yeast	22
Correction to “Toward a Circular Bioeconomy: Designing Microbes and Polymers for Biodegradation”	22
Differentially Optimized Cell-Free Buffer Enables Robust Expression from Unprotected Linear DNA in Exonuclease-Deficient Extracts	22
Discovery of Latent Cannabichromene Cyclase Activity in Marine Bacterial Flavoenzymes	22
Functional Deimmunization of Botulinum Neurotoxin Protease Domain via Computationally Driven Library Design and Ultrahigh-Throughput Screening	22
Biomolecular Condensate-Based Artificial Organelle for Driving Compartmentalized Flux Control	21
Engineering Temperature-Powered Synthetic Multilayer Molecular Bioswitch for High-Level Pyruvate Derivative Production in Escherichia coli	21
Issue Editorial Masthead	21
Metabolic Engineering of Saccharomyces cerevisiae for High-Level Production of l-Pipecolic Acid from Glucose	21
Biosynthesis of the Functional Component Spermidine from Bacillus amyloliquefaciens by Iterative Integration Expression	21
Issue Editorial Masthead	21
Issue Editorial Masthead	21
Imaging Method Using CRISPR/dCas9 and Engineered gRNA Scaffolds Can Perturb Replication Timing at the HSPA1 Locus	21
Transcriptomics-Driven Engineering of Yarrowia lipolytica for Enhanced Fatty Acid Biosynthesis	21
Unraveling the Constrained Cell Growth in Engineered Living Materials	21
Versatile Design of Intracellular Protein-Responsive Translational Regulation System for Synthetic mRNA	21
Robust Synthetic Biology Toolkit to Advance Carboxysome Study and Redesign	20
A Ubiquitous Chromatin Opening Element and DNA Demethylation Facilitate Doxycycline-Controlled Expression during Differentiation and in Transgenic Mice	20

Synthetic Biology of Natural Products Engineering: Recent Advances Across the Discover–Design–Build–Test–Learn Cycle	20
Low Resource Integrated Platform for Production and Analysis of Capped mRNA	20
Synthetic Homoserine Lactone Sensors for Gram-Positive Bacillus subtilis Using LuxR-Type Regulators	20
A Novel Sesquiterpene Synthase from Neophaeococcomyces mojaviensis for α-Santalene Production	20
Encoding Genetic Circuits with DNA Barcodes Paves the Way for Machine Learning-Assisted Metabolite Biosensor Response Curve Profiling in Yeast	20
Point-of-Care Peptide Hormone Production Enabled by Cell-Free Protein Synthesis	20
Pooled CRISPR Interference Screening Identifies Crucial Transcription Factors in Gas-Fermenting Clostridium ljungdahlii	20
HUHgle: An Interactive Substrate Design Tool for Covalent Protein-ssDNA Labeling Using HUH-Tags	20
Highly Reversible Tunable Thermal-Repressible Split-T7 RNA Polymerases (Thermal-T7RNAPs) for Dynamic Gene Regulation	20
Design of Four Small-Molecule-Inducible Systems in the Yeast Chromosome, Applied to Optimize Terpene Biosynthesis	20
Comparison of Kill Switch Toxins in Plant-Beneficial Pseudomonas fluorescens Reveals Drivers of Lethality, Stability, and Escape	20
Conditional Control of Universal CAR T Cells by Cleavable OFF-Switch Adaptors	20
Designing Single-Component Optogenetic Membrane Recruitment Systems: The Rho-Family GTPase Signaling Toolbox	20
Issue Editorial Masthead	20
Engineering a Synthetic Pathway for Tyrosol Synthesis in Escherichia coli	20
A Simple and Highly Sensitive Naked-Eye Analysis of EGFR 19del via CRISPR/Cas12a Triggered No-Nonspecific Nucleic Acid Amplification	20
Genome-Wide CRISPR/Cas9 Screening Unveils a Novel Target ATF7IP–SETDB1 Complex for Enhancing Difficult-to-Express Protein Production	20
Marine Biofilm Engineered to Produce Current in Response to Small Molecules	20
Biosynthesis of Antimicrobial Ornithine-Containing Lacticin 481 Analogues by Use of a Combinatorial Biosynthetic Pathway in Escherichia coli	20
Recombinant Protein Expression Chassis Library of Vibrio natriegens by Fine-Tuning the Expression of T7 RNA Polymerase	20
Tailoring Bacterial Cellulose through the CRISPR/Cas9-Mediated Gene Editing Tool in Komagataeibacter xylinus	19
Statistical Analysis and Tokenization of Epitopes to Construct Artificial Neoepitope Libraries	19
The Translational Coupling of Daidzein Reductase and Dihydrodaidzein Racemase Genes Improves the Production of Equol and Its Analogous Derivatives in Engineered Lactic Acid Bacteria	19
Heterologous Expression and CRISPR/Cas9-Assisted Manipulation of the Hybrid Gene Cluster Specifying the Biosynthesis of Meroterpenoids and Phenazines	19
Metabolic Engineering of Methanotrophic Bacteria for De Novo Production of Taxadiene from Methane	19
Inducible CRISPR/Cas9 Allows for Multiplexed and Rapidly Segregated Single-Target Genome Editing in Synechocystis Sp. PCC 6803	19
Evaluating Apoptotic Gene Efficiency for CHO Culture Performance Using Targeted Integration	19
Issue Publication Information	19
A Simple and Effective Strategy for the Development of Robust Promoter-Centric Gene Expression Tools	19
Engineering Microbial Consortium Biohybrid System to Efficiently Produce Electricity from Lignocellulose Biomass	19
An In Vitro Hybrid Biocatalytic System Enabled by a Combination of Surface-Displayed, Purified, and Cell-Free Expressed Enzymes	19
Spatially Selective Electrochemical Cleavage of a Polymerase-Nucleotide Conjugate	19
Metabolic Engineering of Candida glycerinogenes for Sustainable Production of Geraniol	19
Organellar Glue: A Molecular Tool to Artificially Control Chloroplast–Chloroplast Interactions	19
Engineered Stop and Go T7 RNA Polymerases	19
Production of Caffeic Acid with Co-fermentation of Xylose and Glucose by Multi-modular Engineering in Candida glycerinogenes	19
Machine Learning in Microbiome Research and Engineering	19
Correction to “Hyperaccurate Ribosomes for Improved Genetic Code Reprogramming”	19
Synthetic Ecosystems: From the Test Tube to the Biosphere	18
Reliable Genomic Integration Sites in Pseudomonas putida Identified by Two-Dimensional Transcriptome Analysis	18
Microbial Synthesis of Nucleosides: Advances and Prospects	18
Introduction to Engineering Biology: A Conceptual Framework for Teaching Synthetic Biology	18
GOLDBAR: A Framework for Combinatorial Biological Design	18
The Potential of Artificial Cells Functioning under In Situ Deep-Sea Conditions	18
Engineering a Yeast Cell Factory to Sustainably Biosynthesize Parthenolide	18
Robust Inducible Gene Expression in Intracellular Listeria monocytogenes In Vivo	18
Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and Identification of Potential Bacterial Chassis for Studying Plant–Microbe Interactions	18
A Single-Component Optogenetic Gal4-UAS System Allows Stringent Control of Gene Expression in Zebrafish and Drosophila	18
Antibiotics-Free Steady Bioproduction of Valuable Chemicals from Organic Wastes by Engineered Vibrio natriegens through Targeted Gene Integration	18
Investigating the Specificity of the Dehydration and Cyclization Reactions in Engineered Lanthipeptides by Synechococcal SyncM	18
De Novo Synthesis of Tyrosol and Hydroxytyrosol through Temperature-Inducible Systems and Metabolic Engineering	18
Modular Design of a Copper Ion Biosensor Based on the MAPK Signaling Pathway in Saccharomyces cerevisiae	18
Identifying Conditions for Protein Synthesis Inside Giant Vesicles Using Microfluidics toward Standardized Artificial Cell Production	18
RNA Polymerase III Promoters Compatible with CRISPR Gene Regulation in Saccharomyces cerevisiae	17
Transfer RNA Synthesis-Coupled Translation and DNA Replication in a Reconstituted Transcription/Translation System	17
Sustainable Production of Bio-Based Geraniol: Heterologous Expression of Early Terpenoid Pathway Enzymes in Chlamydomonas reinhardtii	17
Cold Exposure and Oral Delivery of GLP-1R Agonists by an Engineered Probiotic Yeast Strain Have Antiobesity Effects in Mice	17
Conditionally Activated Cross-Linked crRNAs for CRISPR/Cas12a Based Nucleic Acid Detection	17
Biotin-Independent Saccharomyces cerevisiae with Enhanced Growth: Engineering an Acetyl-CoA Carboxylase Bypass	17
Engineering Yarrowia lipolytica to Produce l-Malic Acid from Glycerol	17
Efficient Autoinducible Expression of Recombinant Proteins via the DegSU Quorum Sensing System in a Robust Bacillus subtilis	17
Quantitative Analysis of γ-Aminobutyric Acid by Combined Cell-Free Protein Synthesis and Transamination Reactions	17
Cre/ lox -Mediated Chromosomal Integration of Biosynthetic Gene Clusters for Heterologous Expression in Aspergillus nidulans	17
Rational Engineering of Escherichia coli Nissle 1917 as Live Biotherapeutic to Degrade Uremic Toxin Precursors	17
Combinatorial Engineering of Escherichia coli for Enhancing 3-Fucosyllactose Production	17
Logic Circuits Based on 2A Peptide Sequences in the Yeast Saccharomyces cerevisiae	17
Simple and Efficient Modification of Golden Gate Design Standards and Parts Using Oligo Stitching	17
A Decade of SBOL Visual: Growing Adoption of a Diagram Standard for Engineering Biology	17
One-Pot Biocatalytic Route from Alkanes to α,ω-Diamines by Whole-Cell Consortia of Engineered Yarrowia lipolytica and Escherichia coli	17
Issue Editorial Masthead	17
The iGEM Grand Jamboree and the Serious Fun of Engineering Biology	17
Sense-and-Respond Payload Delivery Using a Novel Antigen-Inducible Promoter Improves Suboptimal CAR-T Activation	17
Cell-Free Expression System Derived from a Near-Minimal Synthetic Bacterium	17
Genetic Code Expansion in Pseudomonas putida KT2440	17
Synthetic Platforms for Characterizing and Targeting of SARS-CoV-2 Genome Capping Enzymes	17
Simulations Reveal High Efficiency and Confinement of a Population Suppression CRISPR Toxin-Antidote Gene Drive	17
GroovDB: A Database of Ligand-Inducible Transcription Factors	17
Issue Publication Information	17
PhiReX 2.0: A Programmable and Red Light-Regulated CRISPR-dCas9 System for the Activation of Endogenous Genes in Saccharomyces cerevisiae	16
CRISPR-GEM: A Novel Machine Learning Model for CRISPR Genetic Target Discovery and Evaluation	16
Combining Fusion of Cells with CRISPR-Cas9 Editing for the Cloning of Large DNA Fragments or Complete Bacterial Genomes in Yeast	16
Construction of a Protein Crystalline Inclusion-Based Enzyme Immobilization System for Biosynthesis of PAPS from ATP and Sulfate	16
Optimization of Hydrogenobyrinic Acid Synthesis in a Cell-Free Multienzyme Reaction by Novel S-Adenosyl-methionine Regeneration	16
Development of a Transcriptional Biosensor for Hydrogen Sulfide That Functions under Aerobic and Anaerobic Conditions	16
Synthesis, Reverse Transcription, Replication, and Inter-Transcription of 2′-Modified Nucleic Acids with Evolved Thermophilic Polymerases: Efforts toward Multidimensional Expansion of the Central Dogm	16
High Titer of (S)-Equol Synthesis from Daidzein in Escherichia coli	16
Engineering Plant Cell Fates and Functions for Agriculture and Industry	16
Sequence-Based Searching for SynBioHub Using VSEARCH	16
Strategy toward In-Cell Self-Assembly of an Artificial Viral Capsid from a Fluorescent Protein-Modified β-Annulus Peptide	16
Optimization of Campesterol-Producing Yeast Strains as a Feasible Platform for the Functional Reconstitution of Plant Membrane-Bound Enzymes	16
Adaptive Laboratory Evolution and Metabolic Engineering of Zymomonas mobilis for Bioethanol Production Using Molasses	16
Autoinduction AND Gate Inhibits Cell Lysis to Enhance Protein Production in Bacillus subtilis Controlled by Population Density and Cell Physiological State	16
Design of a Labile RNase A Using Protein Language Models	16
Establishing an Artificial Pathway for the Biosynthesis of Octopamine and Synephrine	16
Mass Spectrometric Screening for Improving Enzymatic Conversion of Formaldehyde into C2 and C3 Products	16