ACS Synthetic Biology

Article	Citations
Robotic DNA Nanostructures	105
Discovery of Novel Gain-of-Function Mutations Guided by Structure-Based Deep Learning	84
An Automated Model Test System for Systematic Development and Improvement of Gene Expression Models	80
Biosystems Design by Machine Learning	78
Compartmentalizing Cell-Free Systems: Toward Creating Life-Like Artificial Cells and Beyond	72
Transcription-Factor-based Biosensor Engineering for Applications in Synthetic Biology	70
Signal Peptides Generated by Attention-Based Neural Networks	65
Isothermal SARS-CoV-2 Diagnostics: Tools for Enabling Distributed Pandemic Testing as a Means of Supporting Safe Reopenings	64
Statistical Design of Experiments for Synthetic Biology	58
Application of CRISPR-Cas12a Enhanced Fluorescence Assay Coupled with Nucleic Acid Amplification for the Sensitive Detection of African Swine Fever Virus	57
Engineering Yarrowia lipolytica as a Chassis for De Novo Synthesis of Five Aromatic-Derived Natural Products and Chemicals	57
Cell-free Directed Evolution of a Protease in Microdroplets at Ultrahigh Throughput	56
Multicopy Chromosomal Integration Using CRISPR-Associated Transposases	54
Improved Production of Malic Acid in Aspergillus niger by Abolishing Citric Acid Accumulation and Enhancing Glycolytic Flux	53
Engineering Wired Life: Synthetic Biology for Electroactive Bacteria	52
Bio-SCAN: A CRISPR/dCas9-Based Lateral Flow Assay for Rapid, Specific, and Sensitive Detection of SARS-CoV-2	52
Systematic Quantification of Sequence and Structural Determinants Controlling mRNA stability in Bacterial Operons	51
A Unified Dynamic Programming Framework for the Analysis of Interacting Nucleic Acid Strands: Enhanced Models, Scalability, and Speed	50
Synthetic Glycobiology: Parts, Systems, and Applications	50
CRISPR-Mediated Activation of Biosynthetic Gene Clusters for Bioactive Molecule Discovery in Filamentous Fungi	47
CRISPRi-dCas12a: A dCas12a-Mediated CRISPR Interference for Repression of Multiple Genes and Metabolic Engineering in Cyanobacteria	46
Morphological and Metabolic Engineering of Yarrowia lipolytica to Increase β-Carotene Production	46
CRISPR-Cas12a-Assisted Multicolor Biosensor for Semiquantitative Point-of-Use Testing of the Nopaline Synthase Terminator in Genetically Modified Crops by Unaided Eyes	45
Optimized cDICE for Efficient Reconstitution of Biological Systems in Giant Unilamellar Vesicles	44
Optogenetic Amplification Circuits for Light-Induced Metabolic Control	44

Bottom-Up Construction of a Minimal System for Cellular Respiration and Energy Regeneration	43
Enabling Biological Nitrogen Fixation for Cereal Crops in Fertilized Fields	42
A Whole-Cell Biosensor for Point-of-Care Detection of Waterborne Bacterial Pathogens	42
A Dual-Plasmid CRISPR/Cas System for Mycotoxin Elimination in Polykaryotic Industrial Fungi	42
Transcription and Translation in Cytomimetic Protocells Perform Most Efficiently at Distinct Macromolecular Crowding Conditions	41
Cell-Free Gene Expression Dynamics in Synthetic Cell Populations	41
Synthetic Virus-like Particles for Glutathione Biosynthesis	40
Multicopy Targeted Integration for Accelerated Development of High-Producing Chinese Hamster Ovary Cells	40
Automated Continuous Evolution of Proteins in Vivo	40
Rational Promoter Engineering Enables Robust Terpene Production in Microalgae	39
Rewiring Central Carbon Metabolism Ensures Increased Provision of Acetyl-CoA and NADPH Required for 3-OH-Propionic Acid Production	39
CRISPR-Cas13 System as a Promising and Versatile Tool for Cancer Diagnosis, Therapy, and Research	38
Development of Novel Riboswitches for Synthetic Biology in the Green Alga Chlamydomonas	37
Simultaneous Improvement of Limonene Production and Tolerance in Yarrowia lipolytica through Tolerance Engineering and Evolutionary Engineering	37
High-Titer De Novo Biosynthesis of the Predominant Human Milk Oligosaccharide 2′-Fucosyllactose from Sucrose in Escherichia coli	36
3D Bioprinting in Skin Related Research: Recent Achievements and Application Perspectives	36
A User’s Guide to Golden Gate Cloning Methods and Standards	36
Efficient Biosynthesis of (2S)-Eriodictyol from (2S)-Naringenin in Saccharomyces cerevisiae through a Combination of Promoter Adjustment and Directed Evolution	36
CRISPR-dCas9 Mediated Cytosine Deaminase Base Editing in Bacillus subtilis	36
Modular Synthetic Biology Toolkit for Filamentous Fungi	35
Encapsulin Nanocontainers as Versatile Scaffolds for the Development of Artificial Metabolons	35
A Novel Miniature CRISPR-Cas13 System for SARS-CoV-2 Diagnostics	35
Plasmid Vectors for in Vivo Selection-Free Use with the Probiotic E. coli Nissle 1917	34
Design and Characterization of Rapid Optogenetic Circuits for Dynamic Control in Yeast Metabolic Engineering	34
Updated ATLAS of Biochemistry with New Metabolites and Improved Enzyme Prediction Power	34
Dual Regulation of Cytoplasm and Peroxisomes for Improved Α-Farnesene Production in Recombinant Pichia pastoris	34
Optogenetic Control of Microbial Consortia Populations for Chemical Production	34
Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design	34
A Small-Molecule-Responsive Riboswitch Enables Conditional Induction of Viral Vector-Mediated Gene Expression in Mice	34
Improving the Heterologous Production of Fungal Peroxygenases through an Episomal Pichia pastoris Promoter and Signal Peptide Shuffling System	33
High-Level Production of Sesquiterpene Patchoulol in Saccharomyces cerevisiae	33
In Vivo Feedback Control of an Antithetic Molecular-Titration Motif in Escherichia coli Using Microfluidics	33
Rapid Visual CRISPR Assay: A Naked-Eye Colorimetric Detection Method for Nucleic Acids Based on CRISPR/Cas12a and a Convolutional Neural Network	33
Synthetic Biology Toolkit for Marker-Less Integration of Multigene Pathways into Pichia pastoris via CRISPR/Cas9	32
Prime Editing Guide RNA Design Automation Using PINE-CONE	32
In Situ Biomanufacturing of Small Molecules in the Mammalian Gut by Probiotic Saccharomyces boulardii	32
Comprehensive Genome Engineering Toolbox for Microalgae Nannochloropsis oceanica Based on CRISPR-Cas Systems	32
Incorporation of Functional Rubisco Activases into Engineered Carboxysomes to Enhance Carbon Fixation	32
MiniBacillus PG10 as a Convenient and Effective Production Host for Lantibiotics	32
Targeted Diversification in the S. cerevisiae Genome with CRISPR-Guided DNA Polymerase I	32
Rational Design of CRISPR/Cas12a-RPA Based One-Pot COVID-19 Detection with Design of Experiments	32
Functional Identification of Two Types of Carotene Hydroxylases from the Green Alga Dunaliella bardawil Rich in Lutein	32
Evolving a Generalist Biosensor for Bicyclic Monoterpenes	32
CRISPR/Cas-Based Genome Editing for Human Gut Commensal Bacteroides Species	31
Kinetics of RNA and RNA:DNA Hybrid Strand Displacement	31
Engineered Microbial Routes for Human Milk Oligosaccharides Synthesis	30
Engineering Critical Enzymes and Pathways for Improved Triterpenoid Biosynthesis in Yeast	30
Optimized iLID Membrane Anchors for Local Optogenetic Protein Recruitment	30
Programmed Allelic Mutagenesis of a DNA Polymerase with Single Amino Acid Resolution	30
A Golden-Gate Based Cloning Toolkit to Build Violacein Pathway Libraries in Yarrowia lipolytica	29

A Protein-Engineered, Enhanced Yeast Display Platform for Rapid Evolution of Challenging Targets	29
Systematically Engineered Fatty Acid Catabolite Pathway for the Production of (2S)-Naringenin in Saccharomyces cerevisiae	29
Reprogramming Acetogenic Bacteria with CRISPR-Targeted Base Editing via Deamination	29
Metabolic Engineering of Escherichia coli for De Novo Production of 1,5-Pentanediol from Glucose	29
Engineering an Optogenetic CRISPRi Platform for Improved Chemical Production	29
Programmable Fusion and Differentiation of Synthetic Minimal Cells	28
Functional Integration of Two CYP450 Genes Involved in Biosynthesis of Tanshinones for Improved Diterpenoid Production by Synthetic Biology	28
Metabolic Engineering of Gas-Fermenting Clostridium ljungdahlii for Efficient Co-production of Isopropanol, 3-Hydroxybutyrate, and Ethanol	28
Label-Free Detection of Transgenic Crops Using an Isothermal Amplification Reporting CRISPR/Cas12 Assay	28
A Reversibly Induced CRISPRi System Targeting Photosystem II in the Cyanobacterium Synechocystis sp. PCC 6803	27
A Streptomyces venezuelae Cell-Free Toolkit for Synthetic Biology	27
Expanding Toolbox for Genes Expression of Yarrowia lipolytica to Include Novel Inducible, Repressible, and Hybrid Promoters	27
RPA/CRISPR/Cas12a-Based On-Site and Rapid Nucleic Acid Detection of Toxoplasma gondii in the Environment	27
De Novo Biosynthesis of Multiple Pinocembrin Derivatives in Saccharomyces cerevisiae	27
A Low-Cost, Thermostable, Cell-Free Protein Synthesis Platform for On-Demand Production of Conjugate Vaccines	27
Mycoplasma pneumoniae Genome Editing Based on Oligo Recombineering and Cas9-Mediated Counterselection	27
Metabolic Engineering of Saccharomyces cerevisiae for Rosmarinic Acid Production	27
Dynamic Control of 4-Hydroxyisoleucine Biosynthesis by Modified l-Isoleucine Biosensor in Recombinant Corynebacterium glutamicum	27
Building a Versatile Protein Production Platform Using Engineered Trichoderma reesei	26
Systematic Engineering of Escherichia coli for Efficient Production of Nicotinamide Mononucleotide From Nicotinamide	26
Chemical Controllable Gene Drive in Drosophila	26
Biotechnological Production of 2′-Fucosyllactose: A Prevalent Fucosylated Human Milk Oligosaccharide	26
Engineering the Lipid and Fatty Acid Metabolism in Yarrowia lipolytica for Sustainable Production of High Oleic Oils	26
Precise Prediction of Promoter Strength Based on a De Novo Synthetic Promoter Library Coupled with Machine Learning	26
Evolution of Functionally Enhanced α-l-Threofuranosyl Nucleic Acid Aptamers	25
Protecting Linear DNA Templates in Cell-Free Expression Systems from Diverse Bacteria	25
High-Throughput Screening for Substrate Specificity-Adapted Mutants of the Nisin Dehydratase NisB	25
Highly Efficient Production of l-Histidine from Glucose by Metabolically Engineered Escherichia coli	25
YESS 2.0, a Tunable Platform for Enzyme Evolution, Yields Highly Active TEV Protease Variants	25
Thermal Control of Engineered T-cells	25
Constructing Cell-Free Expression Systems for Low-Cost Access	25
Artificial Self-assembling Nanocompartment for Organizing Metabolic Pathways in Yeast	25
De Novo Engineering of Corynebacterium glutamicum for l-Proline Production	25
A Single Cas9-VPR Nuclease for Simultaneous Gene Activation, Repression, and Editing in Saccharomyces cerevisiae	24
Engineering Carboxylic Acid Reductase (CAR) through a Whole-Cell Growth-Coupled NADPH Recycling Strategy	24
High-Performance Allosteric Conditional Guide RNAs for Mammalian Cell-Selective Regulation of CRISPR/Cas	24
The Bacillus subtilis Minimal Genome Compendium	24
Directed Evolution of Ornithine Cyclodeaminase Using an EvolvR-Based Growth-Coupling Strategy for Efficient Biosynthesis of l-Proline	24
Recognition of DNA Target Formulations by CRISPR-Cas12a Using a dsDNA Reporter	24
Efficient Production of 2′-Fucosyllactose from l-Fucose via Self-Assembling Multienzyme Complexes in Engineered Escherichia coli	24
Continuous Cell-Free Replication and Evolution of Artificial Genomic DNA in a Compartmentalized Gene Expression System	24
Membrane Augmented Cell-Free Systems: A New Frontier in Biotechnology	23
Establishment of a Biosensor-based High-Throughput Screening Platform for Tryptophan Overproduction	23
Comprehensive Analysis of Genomic Safe Harbors as Target Sites for Stable Expression of the Heterologous Gene in HEK293 Cells	23
Increased Lipid Production in Yarrowia lipolytica from Acetate through Metabolic Engineering and Cosubstrate Fermentation	23
A Library of Tunable, Portable, and Inducer-Free Promoters Derived from Cyanobacteria	23
Tuning Extracellular Electron Transfer by Shewanella oneidensis Using Transcriptional Logic Gates	23
Optogenetic Control of the BMP Signaling Pathway	23
Cell Synchronization Enhances Nuclear Transformation and Genome Editing via Cas9 Enabling Homologous Recombination in Chlamydomonas reinhardtii	22
Improve the Biosynthesis of Baicalein and Scutellarein via Manufacturing Self-Assembly Enzyme Reactor In Vivo	22
Cheetah: A Computational Toolkit for Cybergenetic Control	22
Reading and Writing Digital Information in TNA	22
Low-Bias Manipulation of DNA Oligo Pool for Robust Data Storage	22
Heterologous Expression of Cryptomaldamide in a Cyanobacterial Host	22
Biosynthesis of a Therapeutically Important Nicotinamide Mononucleotide through a Phosphoribosyl Pyrophosphate Synthetase 1 and 2 Engineered Strain of Escherichia coli	22
Diagnostics of Infections Produced by the Plant Viruses TMV, TEV, and PVX with CRISPR-Cas12 and CRISPR-Cas13	22
Highly Sensitive CRISPR/Cas12a-Based Fluorescence Detection of Porcine Reproductive and Respiratory Syndrome Virus	22
Standardization of Synthetic Biology Tools and Assembly Methods for Saccharomyces cerevisiae and Emerging Yeast Species	22
Aptamer-Mediated Control of Polyadenylation for Gene Expression Regulation in Mammalian Cells	22
evSeq: Cost-Effective Amplicon Sequencing of Every Variant in a Protein Library	21
Optimization of a p-Coumaric Acid Biosensor System for Versatile Dynamic Performance	21
Engineering Yeast Yarrowia lipolytica for Methanol Assimilation	21
Development of a Freeze-Dried CRISPR-Cas12 Sensor for Detecting Wolbachia in the Secondary Science Classroom	21
Genetic Circuit Dynamics: Hazard and Glitch Analysis	21
Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based Applications, Genomic Integrations and Combinatorial Libraries	21
Curli-Mediated Self-Assembly of a Fibrous Protein Scaffold for Hydroxyapatite Mineralization	21
The Marburg Collection: A Golden Gate DNA Assembly Framework for Synthetic Biology Applications in Vibrio natriegens	21
Rational Design of Novel Fluorescent Enzyme Biosensors for Direct Detection of Strigolactones	20
Construction of an l-Tyrosine Chassis in Pichia pastoris Enhances Aromatic Secondary Metabolite Production from Glycerol	20
Construction and Characterization of a Gradient Strength Promoter Library for Fine-Tuned Gene Expression in Bacillus licheniformis	20
Cell Catalysis of Citrate to Itaconate by Engineered Halomonas bluephagenesis	20
Engineering E. coli for Magnetic Control and the Spatial Localization of Functions	20
Systems Metabolic Engineering of Escherichia coli Coculture for De Novo Production of Genistein	20
Artificial ER-Derived Vesicles as Synthetic Organelles for in Vivo Compartmentalization of Biochemical Pathways	20
One-Pot Synthesis of Adipic Acid from Guaiacol in Escherichia coli	19
Constructing a Novel Biosynthetic Pathway for the Production of Glycolate from Glycerol in Escherichia coli	19
DIVERSIFY: A Fungal Multispecies Gene Expression Platform	19
Engineering the Reductive TCA Pathway to Dynamically Regulate the Biosynthesis of Adipic Acid in Escherichia coli	19
Evolving Small-Molecule Biosensors with Improved Performance and Reprogrammed Ligand Preference Using OrthoRep	19
Bringing Light into Cell-Free Expression	19

Automated Rational Strain Construction Based on High-Throughput Conjugation	19
Novel Prokaryotic CRISPR-Cas12a-Based Tool for Programmable Transcriptional Activation and Repression	19
Microbial Synthesis of Human-Hormone Melatonin at Gram Scales	19
Identification of a Strong Quorum Sensing- and Thermo-Regulated Promoter for the Biosynthesis of a New Metabolite Pesticide Phenazine-1-carboxamide in Pseudomonas strain PA1201	19
Combining CRISPR–Cpf1 and Recombineering Facilitates Fast and Efficient Genome Editing in Escherichia coli	19
CRISPRi-Based Dynamic Regulation of Hydrolase for the Synthesis of Poly-γ-Glutamic Acid with Variable Molecular Weights	18
An Aldolase-Based New Pathway for Bioconversion of Formaldehyde and Ethanol into 1,3-Propanediol in Escherichia coli	18
Engineering Antisense Oligonucleotides as Antibacterial Agents That Target FMN Riboswitches and Inhibit the Growth of Staphylococcus aureus, Listeria monocytogenes, and Escherichia co	18
Dynamic Control of Gene Expression with Riboregulated Switchable Feedback Promoters	18
Glycosylation Modification Enhances (2S)-Naringenin Production in Saccharomyces cerevisiae	18
A p-Coumaroyl-CoA Biosensor for Dynamic Regulation of Naringenin Biosynthesis in Saccharomyces cerevisiae	18
Overcoming the Challenges of Megabase-Sized Plasmid Construction in Escherichia coli	18
Functional Comparison of Laboratory-Evolved XNA Polymerases for Synthetic Biology	18
Optogenetic Control of Heterologous Metabolism in E. coli	18
High-Yielding Terpene-Based Biofuel Production in Rhodobacter capsulatus	18
Rerouting Fluxes of the Central Carbon Metabolism and Relieving Mechanism-Based Inactivation of l-Aspartate-α-decarboxylase for Fermentative Production of β-Alanine in Escherichia coli	18
Characterization of a Group of UDP-Glycosyltransferases Involved in the Biosynthesis of Triterpenoid Saponins of Panax notoginseng	18
Design and Reconstruction of Regulatory Parts for Fast-Growing Vibrio natriegens Synthetic Biology	18
Two Completely Orthogonal Quorum Sensing Systems with Self-Produced Autoinducers Enable Automatic Delayed Cascade Control	18
Repurposing the Endogenous Type I-E CRISPR/Cas System for Gene Repression in Gluconobacter oxydans WSH-003	17
Engineering Strategies to Overcome the Stability–Function Trade-Off in Proteins	17
Application of Cell-Free Protein Synthesis System for the Biosynthesis of l-Theanine	17
Emergent Biological Endurance Depends on Extracellular Matrix Composition of Three-Dimensionally Printed Escherichia coli Biofilms	17
Heterologous Expression of Mersacidin in Escherichia coli Elucidates the Mode of Leader Processing	17
A Sensitive and Specific Fluorescent RT-LAMP Assay for SARS-CoV-2 Detection in Clinical Samples	17
Sequence Preference and Initiator Promiscuity for De Novo DNA Synthesis by Terminal Deoxynucleotidyl Transferase	17
Resveratrol Production from Hydrothermally Pretreated Eucalyptus Wood Using Recombinant Industrial Saccharomyces cerevisiae Strains	17
Efficient Production of 1,3-Propanediol from Diverse Carbohydrates via a Non-natural Pathway Using 3-Hydroxypropionic Acid as an Intermediate	17
Distributed Implementation of Boolean Functions by Transcriptional Synthetic Circuits	17
Decoupling Protein Production from Cell Growth Enhances the Site-Specific Incorporation of Noncanonical Amino Acids in E. coli	17
Genome Engineering of the Fast-Growing Mycoplasma feriruminatoris toward a Live Vaccine Chassis	17
Efflux Transporters’ Engineering and Their Application in Microbial Production of Heterologous Metabolites	17
CRISPR/Cas9-Based Genome Editing in the Filamentous Fungus Glarea lozoyensis and Its Application in Manipulating gloF	17
A Novel and Efficient Genome Editing Tool Assisted by CRISPR-Cas12a/Cpf1 for Pichia pastoris	17
FlopR: An Open Source Software Package for Calibration and Normalization of Plate Reader and Flow Cytometry Data	17
Quantitative Yeast–Yeast Two Hybrid for the Discovery and Binding Affinity Estimation of Protein–Protein Interactions	17
Fine-Tuning Multi-Gene Clusters via Well-Characterized Gene Expression Regulatory Elements: Case Study of the Arginine Synthesis Pathway in C. glutamicum	17
High-Titer Production of Olivetolic Acid and Analogs in Engineered Fungal Host Using a Nonplant Biosynthetic Pathway	17
Shaping Liposomes by Cell-Free Expressed Bacterial Microtubules	17
Highly Reversible Tunable Thermal-Repressible Split-T7 RNA Polymerases (Thermal-T7RNAPs) for Dynamic Gene Regulation	16
Orthogonal Blue and Red Light Controlled Cell–Cell Adhesions Enable Sorting-out in Multicellular Structures	16
Light-Induced Patterning of Electroactive Bacterial Biofilms	16
Direct Comparison of d-DNA and l-DNA Strand-Displacement Reactions in Living Mammalian Cells	16
Optimization of Heavy Metal Sensors Based on Transcription Factors and Cell-Free Expression Systems	16
Saccharomyces cerevisiae Synthetic Transcriptional Networks Harnessing dCas12a and Type V-A anti-CRISPR Proteins	16
Optogenetics in Sinorhizobium meliloti Enables Spatial Control of Exopolysaccharide Production and Biofilm Structure	16
Rational Design of Flavonoid Production Routes Using Combinatorial and Precursor-Directed Biosynthesis	16
Synergistic Metabolism of Glucose and Formate Increases the Yield of Short-Chain Organic Acids in Escherichia coli	16
Bioremediation of Industrial Pollutants by Insects Expressing a Fungal Laccase	16
Differentially Optimized Cell-Free Buffer Enables Robust Expression from Unprotected Linear DNA in Exonuclease-Deficient Extracts	16
Development of a Highly Efficient Copper-Inducible GAL Regulation System (CuIGR) in Saccharomyces cerevisiae	16
Living Therapeutics: The Next Frontier of Precision Medicine	16
Metabolic Engineering of Aspartic Acid Supply Modules for Enhanced Production of Bacitracin in Bacillus licheniformis	16
Metabolic Engineering of Saccharomyces cerevisiae for Ethyl Acetate Biosynthesis	16
Engineering T4 Bacteriophage for In Vivo Display by Type V CRISPR-Cas Genome Editing	16
Recombinant Protein Stability in Cyanobacteria	16
Rational Design of a Split Flavin-Based Fluorescent Reporter	16
Cell-Free Characterization of Coherent Feed-Forward Loop-Based Synthetic Genetic Circuits	16
CRISPR-Based Diagnostics: Challenges and Potential Solutions toward Point-of-Care Applications	16
Engineering a Central Carbon Metabolism Pathway to Increase the Intracellular Acetyl-CoA Pool in Synechocystis sp. PCC 6803 Grown under Photomixotrophic Conditions	16
A Computational Framework for Identifying Promoter Sequences in Nonmodel Organisms Using RNA-seq Data Sets	16
Bioengineering Self-Organizing Signaling Centers to Control Embryoid Body Pattern Elaboration	16
Creating Red Light-Switchable Protein Dimerization Systems as Genetically Encoded Actuators with High Specificity	16
De Novo Production of Plant 4′-Deoxyflavones Baicalein and Oroxylin A from Ethanol in Crabtree-Negative Yeast	16
Charge Engineering Improves the Performance of Bst DNA Polymerase Fusions	15
Nisin- and Ripcin-Derived Hybrid Lanthipeptides Display Selective Antimicrobial Activity against Staphylococcus aureus	15
Construction of an rAAV Producer Cell Line through Synthetic Biology	15
GB_SynP: A Modular dCas9-Regulated Synthetic Promoter Collection for Fine-Tuned Recombinant Gene Expression in Plants	15
Efficient Production of a Functional Human Milk Oligosaccharide 3′-Sialyllactose in Genetically Engineered Escherichia coli	15
Targeting glmS Ribozyme with Chimeric Antisense Oligonucleotides for Antibacterial Drug Development	15
Efficient Synthesis of Phycocyanobilin by Combinatorial Metabolic Engineering in Escherichia coli	15
Applications of CRISPR in a Microbial Cell Factory: From Genome Reconstruction to Metabolic Network Reprogramming	15
Activation of Energy Metabolism through Growth Media Reformulation Enables a 24-Hour Workflow for Cell-Free Expression	15
A Lambda Red and FLP/FRT-Mediated Site-Specific Recombination System in Komagataeibacter xylinus and Its Application to Enhance the Productivity of Bacterial Cellulose	15
Engineering a Synthetic Pathway for Tyrosol Synthesis in Escherichia coli	15
Enhancing Homologous Recombination Efficiency in Pichia pastoris for Multiplex Genome Integration Using Short Homology Arms	15
Regulatory Part Engineering for High-Yield Protein Synthesis in an All-Streptomyces-Based Cell-Free Expression System	15
Surface Display of Designer Protein Scaffolds on Genome-Reduced Strains of Pseudomonas putida	15
Actomyosin-Driven Division of a Synthetic Cell	15
Multidimensional Characterization of Parts Enhances Modeling Accuracy in Genetic Circuits	15
Recruiting a Phosphite Dehydrogenase/Formamidase-Driven Antimicrobial Contamination System in Bacillus subtilis for Nonsterilized Fermentation of Acetoin	15
Engineering of a Biosensor in Response to Malate in Bacillus licheniformis	15
A Biosensor for Detection of Indole Metabolites	15
Multilayer Genetic Circuits for Dynamic Regulation of Metabolic Pathways	15
An Aspergillus nidulans Platform for the Complete Cluster Refactoring and Total Biosynthesis of Fungal Natural Products	15
Trade-Offs in Biosensor Optimization for Dynamic Pathway Engineering	15
Engineering Yarrowia lipolytica to Produce Tailored Chain-Length Fatty Acids and Their Derivatives	15
Single Cell Characterization of a Synthetic Bacterial Clock with a Hybrid Feedback Loop Containing dCas9-sgRNA	14
Highly Efficient Production of Menaquinone-7 from Glucose by Metabolically Engineered Escherichia coli	14
Genetic Engineering of Oligotropha carboxidovorans Strain OM5—A Promising Candidate for the Aerobic Utilization of Synthesis Gas	14
Creating RNA Specific C-to-U Editase from APOBEC3A by Separation of Its Activities on DNA and RNA Substrates	14
Evolving a Thermostable Terminal Deoxynucleotidyl Transferase	14
A RAGE Based Strategy for the Genome Engineering of the Human Respiratory Pathogen Mycoplasma pneumoniae	14
DNA Computing: NOT Logic Gates See the Light	14
Macrolide Biosensor Optimization through Cellular Substrate Sequestration	14