OOIR: Observatory of International Research

Papers

(The median citation count of Synthetic Biology is 2. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-10-01 to 2025-10-01.)

Article	Citations
Construction of a syntrophic Pseudomonas putida consortium with reciprocal substrate processing	26
Synthetic biology regulation in Europe: containment, release and beyond	24
Development of synthetic biotics as treatment for human diseases	19
In vitro transcription-based biosensing of glycolate for prototyping of a complex enzyme cascade	18
basicsynbio and the BASIC SEVA collection: software and vectors for an established DNA assembly method	16
Utilizing a cell-free protein synthesis platform for the biosynthesis of a natural product, caffeine	15
Traditional protocols and optimization methods lead to absent expression in a mycoplasma cell-free gene expression platform	10
An artificial protein translation language makes bacteria resistant to viruses	10
An injectable CRISPR therapy instructs B cells to produce anti-HIV antibodies	9
A new Editor-in-chief for Synthetic Biology	9
Construction of a mini-RNA replicon inEscherichia coli	8
Development of an expression-tunable multiple protein synthesis system in cell-free reactions using T7-promoter-variant series	8
A toolkit for enhanced reproducibility of RNASeq analysis for synthetic biologists	8
In- & Out-Cloning: plasmid toolboxes for scarless transcription unit and modular Golden Gate acceptor plasmid assembly	8
A mathematical model of cell-free transcription-translation with plasmid crosstalk	8
GoldenBraid2.0 E. coli: a comprehensive and characterized toolkit for enterics	7
Minimal shuttle vectors for Saccharomyces cerevisiae	7
Automated cell segmentation for reproducibility in bioimage analysis	7
Variability in cell-free expression reactions can impact qualitative genetic circuit characterization	7
Functional Synthetic Biology	6
Fighting fire with fire: engineering a microbe into a therapeutic defense against drug-resistant biofilms	6
Could a simple model of COVID-19 infections be the key to designing better virus-based therapies?	6
Writing the dark matter of the human genome into mice to better replicate human disease	6
Rewiring cell-free metabolic flux inE. colilysates using a block—push—pull approach	6
Cofactor-independent RNA editing by a synthetic S-type PPR protein	5

New gene sensors enable precise cell monitoring and control without altering gene sequence	4
Piece by piece: making plant natural products accessible via heterologous biosynthesis	4
Advancing reproducibility can ease the ‘hard truths’ of synthetic biology	4
BioRxToolbox: a computational framework to streamline genetic circuit design in molecular data communications	4
Engineering a custom-sized DNA scaffold for more efficient DNA origami-based nucleic acid data storage	4
Successful adaptation of a MinION nanopore for protein sequencing	4
Editing Aspergillus terreus using the CRISPR-Cas9 system	4
AssemblyTron: flexible automation of DNA assembly with Opentrons OT-2 lab robots	4
Special issue: reproducibility in synthetic biology	4
CryptKeeper: a negative design tool for reducing unintentional gene expression in bacteria	4
Cell-free expression of RuBisCO for ATP production in the synthetic cells	4
In silico prediction of loop-mediated isothermal amplification using a generalized linear model	4
The transcription activator AtxA from Bacillus anthracis was employed for developing a tight-control, high-level, modulable and stationary-phase-specific transcription activity in Escherichi	3
Biosynthesis of cannabigerol and cannabigerolic acid: the gateways to further cannabinoid production	3
A universal approach to gene expression engineering	3
Evaluating the persistence and stability of a DNA-barcoded microbial system in a mock home environment	3
Cell-free synthesis of infective phages from in vitro assembled phage genomes for efficient phage engineering and production of large phage libraries	3
Signal amplification of araC pBAD using a standardized translation initiation region	3
Phage-delivered CRISPRi enables bacterial biocomputation	2
Engineered microbes report levels of freshwater contamination in real time	2
Screening putative polyester polyurethane degrading enzymes with semi-automated cell-free expression and nitrophenyl probes	2
The naringenin-dependent regulator FdeR can be applied as a NIMPLY gate controlled by naringenin and arabinose	2
Cell-free biosensor with automated acoustic liquid handling for rapid and scalable characterization of cellobiohydrolases on microcrystalline cellulose	2
Biosensor characterization: formal methods from the perspective of proteome fractions	2
Inert splint-driven oligonucleotide assembly	2