OOIR: Observatory of International Research

Papers

(The median citation count of Synthetic Biology is 3. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-04-01 to 2025-04-01.)

Article	Citations
Biosensor characterization: formal methods from the perspective of proteome fractions	70
In- & Out-Cloning: plasmid toolboxes for scarless transcription unit and modular Golden Gate acceptor plasmid assembly	19
The naringenin-dependent regulator FdeR can be applied as a NIMPLY gate controlled by naringenin and arabinose	18
CryptKeeper: a negative design tool for reducing unintentional gene expression in bacteria	17
Highly efficient libraries design for saturation mutagenesis	16
Screening putative polyester polyurethane degrading enzymes with semi-automated cell-free expression and nitrophenyl probes	15
The all-E. coliTXTL toolbox 3.0: new capabilities of a cell-free synthetic biology platform	15
Editing Aspergillus terreus using the CRISPR-Cas9 system	15
Successful adaptation of a MinION nanopore for protein sequencing	15
High-yield ‘one-pot’ biosynthesis of raspberry ketone, a high-value fine chemical	14
Navigating the ‘moral hazard’ argument in synthetic biology’s application	14
In silico Prediction of Loop-Mediated Isothermal Amplification Using a Generalized Linear Model	13
Automated cell segmentation for reproducibility in bioimage analysis	8
Cell-free expression of RuBisCO for ATP production in the synthetic cells	7
Synthetic biology regulation in Europe: containment, release and beyond	7
Sowing the seeds for advanced synthetic plant biology	7
Synthetic biological toggle circuits that respond within seconds and teach us new biology	7
Engineered microbes report levels of freshwater contamination in real time	7
Pathway engineering for high-yield production of lutein in Escherichia coli	6
Data hazards in synthetic biology	6
Data sharing policies: share well and you shall be rewarded	6
Advancing reproducibility can ease the ‘hard truths’ of synthetic biology	6
Variability in cell-free expression reactions can impact qualitative genetic circuit characterization	6
paraSBOLv: a foundation for standard-compliant genetic design visualization tools	5
Standardization of inducer-activated broad host range expression modules: debugging and refactoring an alkane-responsive AlkS/PalkB device	5

BioRxToolbox: a computational framework to streamline genetic circuit design in molecular data communications	5
Contemporary biomedical engineering perspective on volitional evolution for human radiotolerance enhancement beyond low-earth orbit	5
Inert splint-driven oligonucleotide assembly	5
Efficient and iterative retron-mediated in vivo recombineering in Escherichia coli	4
Variability in genome-engineering source materials: consider your starting point	4
Utilizing a cell-free protein synthesis platform for the biosynthesis of a natural product, caffeine	4
Development of synthetic biotics as treatment for human diseases	4
Design and assembly of DNA molecules using multi-objective optimization	4
Rational gRNA design based on transcription factor binding data	4
Biofoundries are a nucleating hub for industrial translation	4
Multicolor plate reader fluorescence calibration	4
In vitro transcription-based biosensing of glycolate for prototyping of a complex enzyme cascade	4
AssemblyTron: flexible automation of DNA assembly with Opentrons OT-2 lab robots	4
A CRISPR/Cas9 method facilitates efficient oligo-mediated gene editing in Debaryomyces hansenii	4
Hurdles in responsive community engagement for the development of environmental biotechnologies	3
basicsynbio and the BASIC SEVA collection: software and vectors for an established DNA assembly method	3
Evaluation of inducible promoter–riboswitch constructs for heterologous protein expression in the cyanobacterial species Anabaena sp. PCC 7120	3
Writing the dark matter of the human genome into mice to better replicate human disease	3
Periplasmic stress contributes to a trade-off between protein secretion and cell growth in Escherichia coli Nissle 1917	3
On the path to generate electricity from wastewater through genetic engineering of Escherichia coli	3
Method for reproducible automated bacterial cell culture and measurement	3
An economy of details: standards and data reusability	3
Self-growing environmentally responsive houses made from agricultural waste and fungal mycelia	3
Xer recombination for the automatic deletion of selectable marker genes from plasmids in enteric bacteria	3
Mouse chromosomes get supersized but find their limits	3