OOIR: Observatory of International Research

Papers

(The median citation count of Synthetic Biology is 2. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2022-01-01 to 2026-01-01.)

Article	Citations
RAPID-DASH: Fast and Efficient Assembly of Guide RNA Arrays for Multiplexed CRISPR-Cas9 Applications	34
Construction of a syntrophic Pseudomonas putida consortium with reciprocal substrate processing	24
Synthetic biology regulation in Europe: containment, release and beyond	21
Development of synthetic biotics as treatment for human diseases	17
In vitro transcription-based biosensing of glycolate for prototyping of a complex enzyme cascade	12
An artificial protein translation language makes bacteria resistant to viruses	11
Utilizing a cell-free protein synthesis platform for the biosynthesis of a natural product, caffeine	11
Traditional protocols and optimization methods lead to absent expression in a mycoplasma cell-free gene expression platform	10
basicsynbio and the BASIC SEVA collection: software and vectors for an established DNA assembly method	10
A new Editor-in-chief for Synthetic Biology	10
A mathematical model of cell-free transcription-translation with plasmid crosstalk	9
A toolkit for enhanced reproducibility of RNASeq analysis for synthetic biologists	9
An injectable CRISPR therapy instructs B cells to produce anti-HIV antibodies	9
Construction of a mini-RNA replicon inEscherichia coli	9
Development of an expression-tunable multiple protein synthesis system in cell-free reactions using T7-promoter-variant series	9
In - & Out-Cloning : plasmid toolboxes for scarless transcription unit and modular Golden Gate acceptor plasmid assembly	8
Minimal shuttle vectors for Saccharomyces cerevisiae	8
Writing the dark matter of the human genome into mice to better replicate human disease	7
Variability in cell-free expression reactions can impact qualitative genetic circuit characterization	7
GoldenBraid2.0 E. coli: a comprehensive and characterized toolkit for enterics	7
Automated cell segmentation for reproducibility in bioimage analysis	7
Harnessing Mass Spectrometry-Based Proteomics for Continuous Directed Evolution	6
Fighting fire with fire: engineering a microbe into a therapeutic defense against drug-resistant biofilms	6
Engineering a custom-sized DNA scaffold for more efficient DNA origami-based nucleic acid data storage	6
Functional Synthetic Biology	6

Rewiring cell-free metabolic flux inE. colilysates using a block—push—pull approach	6
Piece by piece: making plant natural products accessible via heterologous biosynthesis	5
Could a simple model of COVID-19 infections be the key to designing better virus-based therapies?	5
Special issue: reproducibility in synthetic biology	5
New gene sensors enable precise cell monitoring and control without altering gene sequence	5
Cell-free expression of RuBisCO for ATP production in the synthetic cells	4
CryptKeeper: a negative design tool for reducing unintentional gene expression in bacteria	4
Advancing reproducibility can ease the ‘hard truths’ of synthetic biology	4
In silico prediction of loop-mediated isothermal amplification using a generalized linear model	4
Editing Aspergillus terreus using the CRISPR-Cas9 system	4
Successful adaptation of a MinION nanopore for protein sequencing	4
Cell-free synthesis of infective phages from in vitro assembled phage genomes for efficient phage engineering and production of large phage libraries	3
AssemblyTron: flexible automation of DNA assembly with Opentrons OT-2 lab robots	3
Evaluating the persistence and stability of a DNA-barcoded microbial system in a mock home environment	3
BioRxToolbox: a computational framework to streamline genetic circuit design in molecular data communications	3
Signal amplification of araC pBAD using a standardized translation initiation region	3
The transcription activator AtxA from Bacillus anthracis was employed for developing a tight-control, high-level, modulable and stationary-phase-specific transcription activity in Escherichi	2
Screening putative polyester polyurethane degrading enzymes with semi-automated cell-free expression and nitrophenyl probes	2
A universal approach to gene expression engineering	2
Biosensor characterization: formal methods from the perspective of proteome fractions	2
Engineering artificial 5′ regulatory sequences for thermostable protein expression in the extremophile Thermus thermophilus	2
Biosynthesis of cannabigerol and cannabigerolic acid: the gateways to further cannabinoid production	2
The naringenin-dependent regulator FdeR can be applied as a NIMPLY gate controlled by naringenin and arabinose	2
Phage-delivered CRISPRi enables bacterial biocomputation	2