Synthetic and Systems Biotechnology

Article	Citations
Promoting the production of challenging proteins via induced expression in CHO cells and modified cell-free lysates harboring T7 RNA polymerase and mutant eIF2α	85
Nobachelins, new siderophores from Nocardiopsis baichengensis protecting Caenorhabditis elegans from Pseudomonas aeruginosa infection	74
A hybrid RNA-protein biosensor for high-throughput screening of adenosylcobalamin biosynthesis	61
Outside Front Cover	61
Pangenome analysis of Enterobacteria reveals richness of secondary metabolite gene clusters and their associated gene sets	53
Parallel screening and cheminformatics modeling of flavonoid activated aptasensors	47
Metabolic engineering of Glarea lozoyensis for high-level production of pneumocandin B0	44
The biological principles and advanced applications of DSB repair in CRISPR-mediated yeast genome editing	43
Evaluation and optimization of analytical procedure and sample preparation for polar Streptomyces albus J1074 metabolome profiling	42
Quorum sensing inhibition of hordenine analogs on Pseudomonas aeruginosa and Serratia marcescens	41
Biotechnological and food synthetic biology potential of platform strain: Bacillus licheniformis	39
Degradation strategies of pesticide residue: From chemicals to synthetic biology	38
Morphology-engineered alleviation of mycelial aggregation in Streptomyces chassis for potentiated production of secondary metabolites	35
Systemically functional characterization of regiospecific flavonoid O-methyltransferases from Glycine max	35
Cost-effective hybrid long-short read assembly delineates alternative GC-rich Streptomyces hosts for natural product discovery	34
Integration of metabolic and evolutionary processes to construct efficient xylose-utilizing strain of Zymomonas mobilis for lignocellulosic ethanol production	34
DIProT: A deep learning based interactive toolkit for efficient and effective Protein design	32
Thank you Reviewers	31
Microbial production of cis,cis-muconic acid from aromatic compounds in engineered Pseudomonas	30
Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica	30
Single and multiplexed gene repression in solventogenic Clostridium via Cas12a-based CRISPR interference	29
Research progress of pathway and genome evolution in microbes	28
Automated characterization and analysis of expression compatibility between regulatory sequences and metabolic genes in Escherichia coli	27
Achieving robust synthetic tolerance in industrial E. coli through negative auto-regulation of a DsrA-Hfq module	27
Design and construction of microbial cell factories based on systems biology	26

WITHDRAWN: Recent Advances in the Microbial Synthesis of Human Hemoglobin	26
Synergistic Engineering PETase Reveals Loop-Region Mutations for Enhanced Catalytic Activity and Thermal Stability	26
Design and thermodynamic analysis of a pathway enabling anaerobic production of poly-3-hydroxybutyrate in Escherichia coli	26
CgVeA, a light signaling responsive regulator, is involved in regulation of chaetoglobosin A biosynthesis and conidia development in Chaetomium globosum	25
Assessing the physiological properties of baker's yeast based on single-cell Raman spectrum technology	25
Characterization of putative mannoprotein in Kluyveromyces lactis for lactase production	25
Enhanced depolluting capabilities of microbial bioelectrochemical systems by synthetic biology	24
Comparative functional genomics identifies an iron-limited bottleneck in a Saccharomyces cerevisiae strain with a cytosolic-localized isobutanol pathway	23
Engineering Komagataella phaffii to biosynthesize cordycepin from methanol which drives global metabolic alterations at the transcription level	23
Explore the dominant factor in prime editing via a view of DNA processing	23
Cofactor manipulation to drive biosynthesis of natural products	23
Engineering caveolin-mediated endocytosis in Saccharomyces cerevisiae	23
Surface display of carbonic anhydrase on Escherichia coli for CO2 capture and mineralization	22
Synergetic engineering of Escherichia coli for efficient production of l-tyrosine	22
Engineering Yarrowia lipolytica to produce nutritional fatty acids: Current status and future perspectives	22
Efficient genome editing in Claviceps purpurea using a CRISPR/Cas9 ribonucleoprotein method	21
Advances and perspectives in genetic expression and operation for the oleaginous yeast Yarrowia lipolytica	21
Synthetic biotechnology for C1 bio-refinery	21
Polygalacturonase-inhibiting proteins as an exogenously applied natural solution for prevention of postharvest fungal infections	21
Identification of crucial roles of transcription factor IhfA on high production of free fatty acids in Escherichia coli	20
Analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones	19
Engineering and characterization of dehalogenase enzymes from Delftia acidovorans in bioremediation of perfluorinated compounds	19
Systematic metabolic engineering of Yarrowia lipolytica for efficient production of phytohormone abscisic acid	19
Integrating PCR-free amplification and synergistic sensing for ultrasensitive and rapid CRISPR/Cas12a-based SARS-CoV-2 antigen detection	19
CRISPR/Cpf1–FOKI-induced gene editing in Gluconobacter oxydans	19
Toehold switch based biosensors for sensing the highly trafficked rosewood Dalbergia maritima	18
Plant metabolism and synthetic biology	18
Glycosyltransferases: Mining, engineering and applications in biosynthesis of glycosylated plant natural products	18
Investigation of carbonyl amidation and O-methylation during biosynthesis of the pharmacophore pyridyl of antitumor piericidins	18
Improved l-phenylglycine synthesis by introducing an engineered cofactor self-sufficient system	18
Improvement of betanin biosynthesis in Saccharomyces cerevisiae by metabolic engineering	18
Engineering Genetic Elements for Microbial Protein Expression Systems: Advances, Challenges, Applications, and Prospects	17
Increasing prediction performance of colorectal cancer disease status using random forests classification based on metagenomic shotgun sequencing data	17
Protein-protein interface analysis of the non-ribosomal peptide synthetase peptidyl carrier protein and enzymatic domains	17
Transcriptome analysis reveals methanol metabolism variations for the growth damage caused by overexpression of chimeric transactivators in Pichia pastoris	17
Development of multi-epitope mRNA vaccine against Clostridioides difficile using reverse vaccinology and immunoinformatics approaches	17
Putative functions of EpsK in teichuronic acid synthesis and phosphate starvation in Bacillus licheniformis	17
Biochemical synthesis of taxanes from mevalonate	16
Way to efficient microbial paclitaxel mass production	16
Designing a multi-epitope vaccine against Peptostreptococcus anaerobius based on an immunoinformatics approach	16
Metabolic engineering of Bacillus amyloliquefaciens for efficient production of α-glucosidase inhibitor1-deoxynojirimycin	16
A CRISPR/Cas9-based visual toolkit enabling multiplex integration at specific genomic loci in Aspergillus niger	16
A systematic discussion and comparison of the construction methods of synthetic microbial community	15
Engineering the thermostability of d-lyxose isomerase from Caldanaerobius polysaccharolyticus via multiple computer-aided rational design for efficient synthesis of d-mannose	14
CRISPR/Cas12a-based genome editing for cyanophage of Anabeana sp.	14
Genus-specific secondary metabolome in Allokutzneria and Kibdelosporangium	14
Applications of protein engineering in the microbial synthesis of plant triterpenoids	14
Directed evolution of the fusion enzyme for improving astaxanthin biosynthesis in Saccharomyces cerevisiae	14
Engineering Rubisco to enhance CO2 utilization	14
EMT transcription factors activated circuits: A novel tool to study EMT dynamics and its therapeutic implications	13

Harnessing microbial heterogeneity for improved biosynthesis fueled by synthetic biology	13
DNA storage: The future direction for medical cold data storage	13
CO2-elevated cell-free protein synthesis	13
Conversion of acetate and glyoxylate to fumarate by a cell-free synthetic enzymatic biosystem	12
Synthetic biology techniques to tackle heavy metal pollution and poisoning	12
Integrated pathway engineering and transcriptome analysis for improved astaxanthin biosynthesis in Yarrowia lipolytica	12
Laccase is a multitasking protein for synthetic gene circuits in the yeast Saccharomyces cerevisiae	12
Mechanistic insights into the orthogonal functionality of an AHL-mediated quorum-sensing circuit in Yersinia pseudotuberculosis	12
Coordinated regulation of two LacI family regulators, GvmR and GvmR2, on guvermectin production in Streptomyces caniferus	11
MTD: A cloud-based omics database and interactive platform for Myceliophthora thermophila	11
Single carbon metabolism – A new paradigm for microbial bioprocesses?	11
Development of a green Komagataella phaffii cell factory for sustainable production of plant-derived sesquiterpene (–)-α-bisabolol	11
Engineered microbes: Making valuable chemicals from waste gases	11
Extending dynamic and operational range of the biosensor responding to l-carnitine by directed evolution	11
Probing the growth and mechanical properties of Bacillus subtilis biofilms through genetic mutation strategies	11
Microbial synthesis of bacitracin: Recent progress, challenges, and prospects	11
Multi-strategy engineering unusual sugar TDP-l-mycarose biosynthesis to improve the production of 3-O-α-mycarosylerythronolide B in Escherichia coli	11
Transcriptome-wide analysis of PIP reductase gene family identified a phenylpropene synthase crucial for the biosynthesis of dibenzocyclooctadiene lignans in Schisandra chinensis	11
A statistical approach to enhance the productivity of Streptomyces baarensis MH-133 for bioactive compounds	11
Simulating androgen receptor selection in designer yeast	11
ECMpy 2.0: A Python package for automated construction and analysis of enzyme-constrained models	10
Identification, characterization, and application of an NADPH-dependent carbonyl reductase in Saccharomyces cerevisiae AS2.346 for improving production of (13R,17S)-ethyl secol	10
Biotransformation of ethylene glycol by engineered Escherichia coli	10
A flexible, modular and versatile functional part assembly toolkit for gene cluster engineering in Streptomyces	10
Construction and application of high-quality genome-scale metabolic model of Zymomonas mobilis to guide rational design of microbial cell factories	10
CRISPRi-mediated multigene downregulating redirects the metabolic flux to spinosad biosynthesis in Saccharopolyspora spinosa	10
A combined strategy for the overproduction of complex ergot alkaloid agroclavine	10
Advances in the biosynthesis of tetraacetyl phytosphingosine, a key substrate of ceramides	10
Exploration and mutagenesis of the germacrene A synthase from Solidago canadensis to enhance germacrene A production in E. coli	10
New dual functional CYP450 gene involves in isoflavone biosynthesis in Glycine max L.	10
Identification of neutral genome integration sites with high expression and high integration efficiency in Fusarium venenatum TB01	10
Developing safe and efficient CGBE editor based on Cas-embedding strategy	10
Development of ultrasound-visualized tumor-targeting engineered bacteria for precise tumor therapy	9
Enhancing abscisic acid production in Botrytis cinerea through metabolic engineering based on a constitutive promoter library	9
Enhanced triacylglycerol metabolism contributes to the efficient biosynthesis of spinosad in Saccharopolyspora spinosa	9
A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris	9
Programming anti-ribozymes to sense trigger RNAs for modulating gene expression in mammalian cells	9
Transcriptome-guided identification of a four-component system, SbrH1-R, that modulates milbemycin biosynthesis by influencing gene cluster expression, precursor supply, and antibiotic efflux	9
Deacetoxycephalosporin C synthase (expandase): Research progress and application potential	9
Translocation of subunit PPSE in plipastatin synthase and synthesis of novel lipopeptides	9
Sequence and taxonomic feature evaluation facilitated the discovery of alcohol oxidases	9
Advances in stress-tolerance elements for microbial cell factories	9
Minimizing endogenous cryptic plasmids to construct antibiotic-free expression systems for Escherichia coli Nissle 1917	8
Corrigendum to “Improving cooperativity of transcription activators by oligomerization domains in mammalian cells” [Synth Syst Biotechnol 8 (1) (2023) 114–120]	8
Screening of ent-copalyl diphosphate synthase and metabolic engineering to achieve de novo biosynthesis of ent-copalol in Saccharomyces cerevisiae	8
The forced activation of asexual conidiation in Aspergillus niger simplifies bioproduction	8
Efficient production of astaxanthin in Yarrowia lipolytica through metabolic and enzyme engineering	8
Engineering transcriptional regulatory networks for improving second-generation fuel ethanol production in Saccharomyces cerevisiae	8
Improving the biotransformation efficiency of soybean phytosterols in Mycolicibacterium neoaurum by the combined deletion of fbpC3 and embC in cell envelope synthesis	8
Engineered probiotic-mediated intratumoral delivery and controlled release of bacterial collagenase for cancer therapy	8
CRISPR/Cas9-based iterative multi-copy integration for improved metabolite yields in Saccharomyces cerevisiae	8
Engineering Corynebacterium glutamicum for the efficient production of 3-hydroxypropionic acid from glucose via the β-alanine pathway	8
Improving pathway prediction accuracy of constraints-based metabolic network models by treating enzymes as microcompartments	8
High-efficiency patatin expression strategies in Komagataella phaffii (Pichia pastoris): Expression cassette toolbox and regulation of protein secretion	8
Cyanamide-inducible expression of homing nuclease I−SceI for selectable marker removal and promoter characterisation in Saccharomyces cerevisiae	8
Filamentous morphology engineering of bacteria by iron metabolism modulation through MagR expression	8
Modular engineering of E. coli coculture for efficient production of resveratrol from glucose and arabinose mixture	8
System-level characterization of engineered and evolved formatotrophic E. coli strains	7
Outside Front Cover	7
A two-stage metabolome refining pipeline for natural products discovery	7
Enhancing substrate specificity of microbial transglutaminase for precise nanobody labeling	7
Integrative analysis of transcriptome and metabolome reveals the effect of DNA methylation of chalcone isomerase gene in promoter region on Lithocarpus polystachyus Rehd flavonoids	7
Efficient fatty acid synthesis from methanol in methylotrophic yeast	7
Regulatory RNAs in Bacillus subtilis: A review on regulatory mechanism and applications in synthetic biology	7
Two novel aliphatic unsaturated alcohols isolated from a pathogenic fungus Fusarium proliferatum	7
Biomanufacturing of γ-linolenic acid-enriched galactosyldiacylglycerols: Challenges in microalgae and potential in oleaginous yeasts	7
CRISPR-Cas9-based one-step multiplexed genome editing through optimizing guide RNA processing strategies in Pichia pastoris	7
Facilitating stable gene integration expression and copy number amplification in Bacillus subtilis through a reversible homologous recombination switch	7
Analyzing and engineering of the biosynthetic pathway of mollemycin A for enhancing its production	7
Regulatory mechanisms of dopamine metabolism in a marine Meyerozyma guilliermondii GXDK6 under NaCl stress as revealed by integrative multi-omics analysis	7
Thank you Reviewers list	7
A new paradigm for the regulation of A40926B0 biosynthesis	7
Sequence and thermodynamic characteristics of terminators revealed by FlowSeq and the discrimination of terminators strength	7
Multiscale engineering of microbial cell factories: A step forward towards sustainable natural products industry	7
Identification of a novel multifunctional oxidosqualene cyclase from Zea mays sheds light on the biosynthetic pathway of three pentacyclic triterpenoids	7
Modulating ion channels with nanobodies	6
A FAIR-compliant parts catalogue for genome engineering and expression control in Saccharomyces cerevisiae	6
Biosensor-assisted CRISPRi high-throughput screening to identify genetic targets in Zymomonas mobilis for high d-lactate production	6
Optimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies	6
Acarbose glycosylation by AcbE for the production of acarstatins with enhanced α-amylase inhibitory activity	6

Construction of an engineered Escherichia coli for effective synthesis of 2′-fucosyllactose via the salvage pathway	6
Deletion of COM donor and acceptor domains and the interaction between modules in bacillomycin D produced by Bacillus amyloliquefaciens	6
Overexpression of arginase gene CAR1 renders yeast Saccharomyces cerevisiae acetic acid tolerance	6
Biosynthesis of trialkyl-substituted aromatic polyketide NFAT-133 involves unusual P450 monooxygenase-mediating aromatization and a putative metallo-beta-lactamase fold hydrolase	6
High-level production of free fatty acids from lignocellulose hydrolysate by co-utilizing glucose and xylose in yeast	6
Enhancement of polymyxin B1 production by an artificial microbial consortium of Paenibacillus polymyxa and recombinant Corynebacterium glutamicum producing precursor amino acids	6
Alleviating glucose repression and enhancing respiratory capacity to increase itaconic acid production	6
Multi-modular metabolic engineering of heme synthesis in Corynebacterium glutamicum	6
Enhanced chlorogenic acid production from glucose via systematic metabolic engineering of Saccharomyces cerevisiae	6
Study on RNAi-based herbicide for Mikania micrantha	6
Nitric oxide synthase-guided genome mining identifies a cytochrome P450 enzyme for olefin nitration in bacterial specialized metabolism	6
Combined metabolic and enzymatic engineering for de novo biosynthesis of δ-tocotrienol in Yarrowia lipolytica	6
T7 RNA polymerase-guided base editor for accelerated continuous evolution in Bacillus subtilis	6
Current state and future perspectives of cytochrome P450 enzymes for C–H and C=C oxygenation	6
Construction of microbial chassis for terpenoid discovery	6
Identification of (-)-bornyl diphosphate synthase from Blumea balsamifera and its application for (-)-borneol biosynthesis in Saccharomyces cerevisiae	6
Rational construction of genome-minimized Streptomyces host for the expression of secondary metabolite gene clusters	6
Systems metabolic engineering of Corynebacterium glutamicum for efficient l-tryptophan production	6
Altered gut bacterial and metabolic signatures and their interaction in inflammatory bowel disease	6
End-to-end computational approach to the design of RNA biosensors for detecting miRNA biomarkers of cervical cancer	6
Constructing an artificial short route for cell-free biosynthesis of the phenethylisoquinoline scaffold	6
iCASRED, a scarless DNA editing tool in E. coli for high-efficiency engineering of natural product biosynthetic gene clusters	6
Artificial multi-enzyme cascades and whole-cell transformation for bioconversion of C1 compounds: Advances, challenge and perspectives	6
Network of nicotinamide mononucleotide biosynthesis: substrate tracking, rate-limiting enzyme, and regulatory strategy	6
Editorial for “Biointelligent manufacturing in Memorium of Arnold (Arny) L. Demain”	5
Design and mathematical analysis of activating transcriptional amplifiers that enable modular temporal control in synthetic juxtacrine circuits	5
Expanding catalytic promiscuity of a bifunctional terpene synthase through a single mutation-induced change in hydrogen-bond network within the catalytic pocket	5
Thank you Reviewers	5
Mechanisms and biotechnological applications of transcription factors	5
Biodegradation of combined pollutants of polyethylene terephthalate and phthalate esters by esterase-integrated Pseudomonas sp. JY-Q with surface-co-displayed PETase and MHETase	5
Polyhydroxyalkanoate production during electroactive biofilm formation and stabilization in wetland microbial fuel cells for petroleum hydrocarbon bioconversion	5
Biosynthesis of Lacto-N-biose I from starch and N-acetylglucosamine via an in vitro synthetic enzymatic biosystem	5
Discovering the next decade's synthetic biology research trends with ChatGPT	5
Efficient secretory expression of phospholipase D for the high-yield production of phosphatidylserine and phospholipid derivates from soybean lecithin	5
Improving solubility and copy number of taxadiene synthase to enhance the titer of taxadiene in Yarrowia lipolytica	5
Efficient biosynthesis of creatine by whole-cell catalysis from guanidinoacetic acid in Corynebacterium glutamicum	5
Recent progress in engineering Clostridium autoethanogenum to synthesize the biochemicals and biocommodities	5
Efficient stereoselective hydroxylation of deoxycholic acid by the robust whole-cell cytochrome P450 CYP107D1 biocatalyst	5
Design and engineering of logic genetic-enzymatic gates based on the activity of the human CYP2C9 enzyme in permeabilized Saccharomyces cerevisiae cells	5
Enhancing levan biosynthesis by destroying the strongly acidic environment caused by membrane-bound glucose dehydrogenase (mGDH) in Gluconobacter sp. MP2116	5
Stepwise increase of fidaxomicin in an engineered heterologous host Streptomyces albus through multi-level metabolic engineering	5
Improving cooperativity of transcription activators by oligomerization domains in mammalian cells	5
Efficient production of hydroxysalidroside in Escherichia coli via enhanced glycosylation and semi-rational design of UGT85A1	4
Engineering artificial cross-species promoters with different transcriptional strengths	4
finDr: A web server for in silico D-peptide ligand identification	4
Engineering the cytochrome P450 to enhance parthenolide production in Saccharomyces cerevisiae	4
Characterizing and engineering promoters for metabolic engineering of Ogataea polymorpha	4
Biosensors for the detection of flaviviruses: A review	4
Efficient biosynthesis of β-caryophyllene in Saccharomyces cerevisiae by β-caryophyllene synthase from Artemisia argyi	4
Engineered microorganisms: A new direction in kidney stone prevention and treatment	4
The potential of plant proteins as antifungal agents for agricultural applications	4
Advance of tolerance engineering on microbes for industrial production	4
Corrigendum to “Aptamer-based cell-free detection system to detect target protein” [Synth Syst Biotechnol 6 (3) (2021) 209–215]	4
Antibacterial activity and mechanism of X33 antimicrobial oligopeptide against Acinetobacter baumannii	4
Reconstruction and metabolic profiling of the genome-scale metabolic network model of Pseudomonas stutzeri A1501	4
Functional characterization of triterpene synthases in Cibotium barometz	4
The LysR family transcriptional regulator ORF-L16 regulates spinosad biosynthesis in Saccharopolyspora spinosa	4
Novel switchable ECF sigma factor transcription system for improving thaxtomin A production in Streptomyces	4
Strategies to increase tolerance and robustness of industrial microorganisms	4
Fragment antigen binding domains (Fabs) as tools to study assembly-line polyketide synthases	4
Gene knockdown by structure defined single-stem loop small non-coding RNAs with programmable regulatory activities	4
H-NS family protein MvaU downregulates phenazine-1-carboxylic acid (PCA) biosynthesis via binding to an AT-rich region within the promoter of the phz2 gene cluster in the rhizobacterium Pseudomonas st	4
dTSR enables efficient improvement of heterologous production of spinosad in Saccharopolyspora erythraea	4
Outside Front Cover	4
Origin and evolution of the main starch biosynthetic enzymes	4
Enzymatic properties and inhibition tolerance analysis of key enzymes in β-phenylethanol anabolic pathway of Saccharomyces cerevisiae HJ	4
Multiplexed site-specific genome engineering in Mycolicibacterium neoaurum by Att/Int system	4
Metabolic engineering of an industrial bacterium Zymomonas mobilis for anaerobic l-serine production	4