Synthetic and Systems Biotechnology

Article	Citations
Promoting the production of challenging proteins via induced expression in CHO cells and modified cell-free lysates harboring T7 RNA polymerase and mutant eIF2α	94
Outside Front Cover	76
A hybrid RNA-protein biosensor for high-throughput screening of adenosylcobalamin biosynthesis	66
Metabolic engineering of Glarea lozoyensis for high-level production of pneumocandin B0	64
Nobachelins, new siderophores from Nocardiopsis baichengensis protecting Caenorhabditis elegans from Pseudomonas aeruginosa infection	57
Parallel screening and cheminformatics modeling of flavonoid activated aptasensors	52
Pangenome analysis of Enterobacteria reveals richness of secondary metabolite gene clusters and their associated gene sets	47
Genomic and transcriptomic insights into the molecular responses of a biocrust-derived oleaginous microalga Vischeria sp. WL1 to nitrogen depletion and recovery	43
The biological principles and advanced applications of DSB repair in CRISPR-mediated yeast genome editing	43
Evaluation and optimization of analytical procedure and sample preparation for polar Streptomyces albus J1074 metabolome profiling	39
Degradation strategies of pesticide residue: From chemicals to synthetic biology	38
Thank you Reviewers	35
Biotechnological and food synthetic biology potential of platform strain: Bacillus licheniformis	35
Integration of metabolic and evolutionary processes to construct efficient xylose-utilizing strain of Zymomonas mobilis for lignocellulosic ethanol production	34
Research progress of pathway and genome evolution in microbes	33
Cost-effective hybrid long-short read assembly delineates alternative GC-rich Streptomyces hosts for natural product discovery	32
Design and construction of microbial cell factories based on systems biology	30
Automated characterization and analysis of expression compatibility between regulatory sequences and metabolic genes in Escherichia coli	30
DIProT: A deep learning based interactive toolkit for efficient and effective Protein design	30
Achieving robust synthetic tolerance in industrial E. coli through negative auto-regulation of a DsrA-Hfq module	30
Morphology-engineered alleviation of mycelial aggregation in Streptomyces chassis for potentiated production of secondary metabolites	30
Systemically functional characterization of regiospecific flavonoid O-methyltransferases from Glycine max	30
Single and multiplexed gene repression in solventogenic Clostridium via Cas12a-based CRISPR interference	30
Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica	28
Microbial production of cis,cis-muconic acid from aromatic compounds in engineered Pseudomonas	28

Improving fidaxomicin production through ARTP mutagenesis and fermentation optimization in Actinoplanes deccanensis	27
WITHDRAWN: Recent Advances in the Microbial Synthesis of Human Hemoglobin	26
Surface display of carbonic anhydrase on Escherichia coli for CO2 capture and mineralization	26
Characterization of putative mannoprotein in Kluyveromyces lactis for lactase production	25
Cofactor manipulation to drive biosynthesis of natural products	25
Comparative functional genomics identifies an iron-limited bottleneck in a Saccharomyces cerevisiae strain with a cytosolic-localized isobutanol pathway	25
Explore the dominant factor in prime editing via a view of DNA processing	24
Engineering Yarrowia lipolytica to produce nutritional fatty acids: Current status and future perspectives	24
CgVeA, a light signaling responsive regulator, is involved in regulation of chaetoglobosin A biosynthesis and conidia development in Chaetomium globosum	24
Design and thermodynamic analysis of a pathway enabling anaerobic production of poly-3-hydroxybutyrate in Escherichia coli	24
Engineering caveolin-mediated endocytosis in Saccharomyces cerevisiae	23
Recombinant production of amaranthin and other betalain variants with yeast cell factories	23
Enhanced depolluting capabilities of microbial bioelectrochemical systems by synthetic biology	23
Assessing the physiological properties of baker's yeast based on single-cell Raman spectrum technology	22
Engineering Komagataella phaffii to biosynthesize cordycepin from methanol which drives global metabolic alterations at the transcription level	22
Synergetic engineering of Escherichia coli for efficient production of l-tyrosine	21
Synthetic biotechnology for C1 bio-refinery	20
CRISPR/Cpf1–FOKI-induced gene editing in Gluconobacter oxydans	19
A multifunctional switch for label-free CRISPR/Cas12a sensor with self-driven amplification	19
Advances and perspectives in genetic expression and operation for the oleaginous yeast Yarrowia lipolytica	19
Engineering non-P450 3-hydroxylase for de novo synthesizes catechol-containing compounds in Escherichia coli	19
Polygalacturonase-inhibiting proteins as an exogenously applied natural solution for prevention of postharvest fungal infections	19
Engineering and characterization of dehalogenase enzymes from Delftia acidovorans in bioremediation of perfluorinated compounds	18
Systematic metabolic engineering of Yarrowia lipolytica for efficient production of phytohormone abscisic acid	18
Efficient genome editing in Claviceps purpurea using a CRISPR/Cas9 ribonucleoprotein method	18
Glycosyltransferases: Mining, engineering and applications in biosynthesis of glycosylated plant natural products	18
Identification of crucial roles of transcription factor IhfA on high production of free fatty acids in Escherichia coli	18
Toehold switch based biosensors for sensing the highly trafficked rosewood Dalbergia maritima	18
Investigation of carbonyl amidation and O-methylation during biosynthesis of the pharmacophore pyridyl of antitumor piericidins	17
Protein-protein interface analysis of the non-ribosomal peptide synthetase peptidyl carrier protein and enzymatic domains	17
Putative functions of EpsK in teichuronic acid synthesis and phosphate starvation in Bacillus licheniformis	17
Metabolic engineering of Bacillus amyloliquefaciens for efficient production of α-glucosidase inhibitor1-deoxynojirimycin	17
Improved l-phenylglycine synthesis by introducing an engineered cofactor self-sufficient system	16
Plant metabolism and synthetic biology	16
Improvement of betanin biosynthesis in Saccharomyces cerevisiae by metabolic engineering	15
Way to efficient microbial paclitaxel mass production	15
Development of multi-epitope mRNA vaccine against Clostridioides difficile using reverse vaccinology and immunoinformatics approaches	15
Increasing prediction performance of colorectal cancer disease status using random forests classification based on metagenomic shotgun sequencing data	15
From consultors to collaborators – An SOP for advancing ethics engagement in science	15
Transcriptome analysis reveals methanol metabolism variations for the growth damage caused by overexpression of chimeric transactivators in Pichia pastoris	15
Biochemical synthesis of taxanes from mevalonate	14
A systematic discussion and comparison of the construction methods of synthetic microbial community	13
Designing a multi-epitope vaccine against Peptostreptococcus anaerobius based on an immunoinformatics approach	13
Probing the growth and mechanical properties of Bacillus subtilis biofilms through genetic mutation strategies	13
Transcriptome-wide analysis of PIP reductase gene family identified a phenylpropene synthase crucial for the biosynthesis of dibenzocyclooctadiene lignans in Schisandra chinensis	13
CRISPR/Cas12a-based genome editing for cyanophage of Anabeana sp.	13
Directed evolution of the fusion enzyme for improving astaxanthin biosynthesis in Saccharomyces cerevisiae	13
Genus-specific secondary metabolome in Allokutzneria and Kibdelosporangium	13
Engineering Rubisco to enhance CO2 utilization	13
Applications of protein engineering in the microbial synthesis of plant triterpenoids	13

A CRISPR/Cas9-based visual toolkit enabling multiplex integration at specific genomic loci in Aspergillus niger	13
Conversion of acetate and glyoxylate to fumarate by a cell-free synthetic enzymatic biosystem	13
Engineering T7 RNA polymerase-cascaded systems controlled by nisin and theophylline for protein overexpression and targeted gene mutagenesis in Lactococcus lactis	12
Integrated pathway engineering and transcriptome analysis for improved astaxanthin biosynthesis in Yarrowia lipolytica	12
Harnessing microbial heterogeneity for improved biosynthesis fueled by synthetic biology	12
Metabolic engineering Saccharomyces cerevisiae for de novo biosynthesis of hydroxycinnamoyl glycerols	12
CO2-elevated cell-free protein synthesis	12
Single carbon metabolism – A new paradigm for microbial bioprocesses?	12
Engineering the thermostability of d-lyxose isomerase from Caldanaerobius polysaccharolyticus via multiple computer-aided rational design for efficient synthesis of d-mannose	12
Mechanistic insights into the orthogonal functionality of an AHL-mediated quorum-sensing circuit in Yersinia pseudotuberculosis	12
DNA storage: The future direction for medical cold data storage	12
Laccase is a multitasking protein for synthetic gene circuits in the yeast Saccharomyces cerevisiae	12
Synthetic biology techniques to tackle heavy metal pollution and poisoning	11
MTD: A cloud-based omics database and interactive platform for Myceliophthora thermophila	11
EMT transcription factors activated circuits: A novel tool to study EMT dynamics and its therapeutic implications	11
A statistical approach to enhance the productivity of Streptomyces baarensis MH-133 for bioactive compounds	11
New dual functional CYP450 gene involves in isoflavone biosynthesis in Glycine max L.	11
Simulating androgen receptor selection in designer yeast	11
Coordinated regulation of two LacI family regulators, GvmR and GvmR2, on guvermectin production in Streptomyces caniferus	11
Development of a green Komagataella phaffii cell factory for sustainable production of plant-derived sesquiterpene (–)-α-bisabolol	11
Microbial synthesis of bacitracin: Recent progress, challenges, and prospects	11
Engineered microbes: Making valuable chemicals from waste gases	11
Extending dynamic and operational range of the biosensor responding to l-carnitine by directed evolution	11
ECMpy 2.0: A Python package for automated construction and analysis of enzyme-constrained models	11
Identification, characterization, and application of an NADPH-dependent carbonyl reductase in Saccharomyces cerevisiae AS2.346 for improving production of (13R,17S)-ethyl secol	10
Construction and application of high-quality genome-scale metabolic model of Zymomonas mobilis to guide rational design of microbial cell factories	10
A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris	10
Advances in the biosynthesis of tetraacetyl phytosphingosine, a key substrate of ceramides	10
Developing safe and efficient CGBE editor based on Cas-embedding strategy	10
CRISPRi-mediated multigene downregulating redirects the metabolic flux to spinosad biosynthesis in Saccharopolyspora spinosa	10
A combined strategy for the overproduction of complex ergot alkaloid agroclavine	10
Multi-strategy engineering unusual sugar TDP-l-mycarose biosynthesis to improve the production of 3-O-α-mycarosylerythronolide B in Escherichia coli	10
Exploration and mutagenesis of the germacrene A synthase from Solidago canadensis to enhance germacrene A production in E. coli	10
Identification of neutral genome integration sites with high expression and high integration efficiency in Fusarium venenatum TB01	10
Systematic design and evaluation of artificial CO2 assimilation pathways	9
Sequence and taxonomic feature evaluation facilitated the discovery of alcohol oxidases	9
Transcriptome-guided identification of a four-component system, SbrH1-R, that modulates milbemycin biosynthesis by influencing gene cluster expression, precursor supply, and antibiotic efflux	9
A flexible, modular and versatile functional part assembly toolkit for gene cluster engineering in Streptomyces	9
Decoupled tricarboxylic acid cycle and glycolysis of Corynebacterium glutamicum combined with acetate supply promote high-yield biosynthesis of l-homoserine	9
Programming anti-ribozymes to sense trigger RNAs for modulating gene expression in mammalian cells	9
De novo biosynthesis of Asperosaponin VI in Saccharomyces cerevisiae	9
Biotransformation of ethylene glycol by engineered Escherichia coli	9
Development of ultrasound-visualized tumor-targeting engineered bacteria for precise tumor therapy	9
Advances in stress-tolerance elements for microbial cell factories	8
Filamentous morphology engineering of bacteria by iron metabolism modulation through MagR expression	8
Regulatory mechanisms of dopamine metabolism in a marine Meyerozyma guilliermondii GXDK6 under NaCl stress as revealed by integrative multi-omics analysis	8
CRISPR/Cas9-based iterative multi-copy integration for improved metabolite yields in Saccharomyces cerevisiae	8
Engineering Corynebacterium glutamicum for the efficient production of 3-hydroxypropionic acid from glucose via the β-alanine pathway	8
Cyanamide-inducible expression of homing nuclease I−SceI for selectable marker removal and promoter characterisation in Saccharomyces cerevisiae	8
Translocation of subunit PPSE in plipastatin synthase and synthesis of novel lipopeptides	8
Screening of ent-copalyl diphosphate synthase and metabolic engineering to achieve de novo biosynthesis of ent-copalol in Saccharomyces cerevisiae	8
Engineered probiotic-mediated intratumoral delivery and controlled release of bacterial collagenase for cancer therapy	8
Enhanced triacylglycerol metabolism contributes to the efficient biosynthesis of spinosad in Saccharopolyspora spinosa	8
Development and application of synthetic biology tools for improved production of human breast milk components	8
Enhancing abscisic acid production in Botrytis cinerea through metabolic engineering based on a constitutive promoter library	8
The forced activation of asexual conidiation in Aspergillus niger simplifies bioproduction	8
Enhancing 3-hydroxypropionic acid production from recombinant Saccharomyces cerevisiae for using rice straw hydrolysate and sugar cane industrial waste as substrate	8
Efficient production of astaxanthin in Yarrowia lipolytica through metabolic and enzyme engineering	8
Engineering transcriptional regulatory networks for improving second-generation fuel ethanol production in Saccharomyces cerevisiae	7
Corrigendum to “Improving cooperativity of transcription activators by oligomerization domains in mammalian cells” [Synth Syst Biotechnol 8 (1) (2023) 114–120]	7
Engineering Yarrowia lipolytica for high-level β-elemene production via combinatorial metabolic strategies	7
Biomanufacturing of γ-linolenic acid-enriched galactosyldiacylglycerols: Challenges in microalgae and potential in oleaginous yeasts	7
Improving pathway prediction accuracy of constraints-based metabolic network models by treating enzymes as microcompartments	7
High-efficiency patatin expression strategies in Komagataella phaffii (Pichia pastoris): Expression cassette toolbox and regulation of protein secretion	7
Minimizing endogenous cryptic plasmids to construct antibiotic-free expression systems for Escherichia coli Nissle 1917	7
Outside Front Cover	7
Thank you Reviewers list	7
Modular engineering of E. coli coculture for efficient production of resveratrol from glucose and arabinose mixture	7
Regulatory RNAs in Bacillus subtilis: A review on regulatory mechanism and applications in synthetic biology	7
Improving the biotransformation efficiency of soybean phytosterols in Mycolicibacterium neoaurum by the combined deletion of fbpC3 and embC in cell envelope synthesis	7
A new paradigm for the regulation of A40926B0 biosynthesis	7
Enhancing substrate specificity of microbial transglutaminase for precise nanobody labeling	7
Sequence and thermodynamic characteristics of terminators revealed by FlowSeq and the discrimination of terminators strength	6
Acarbose glycosylation by AcbE for the production of acarstatins with enhanced α-amylase inhibitory activity	6
Construction of an engineered Escherichia coli for effective synthesis of 2′-fucosyllactose via the salvage pathway	6
Optimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies	6
A FAIR-compliant parts catalogue for genome engineering and expression control in Saccharomyces cerevisiae	6
Alleviating glucose repression and enhancing respiratory capacity to increase itaconic acid production	6
End-to-end computational approach to the design of RNA biosensors for detecting miRNA biomarkers of cervical cancer	6
Multiscale engineering of microbial cell factories: A step forward towards sustainable natural products industry	6

Identification of a novel multifunctional oxidosqualene cyclase from Zea mays sheds light on the biosynthetic pathway of three pentacyclic triterpenoids	6
CRISPR-Cas9-based one-step multiplexed genome editing through optimizing guide RNA processing strategies in Pichia pastoris	6
Systems metabolic engineering of Corynebacterium glutamicum for efficient l-tryptophan production	6
High-level production of free fatty acids from lignocellulose hydrolysate by co-utilizing glucose and xylose in yeast	6
Biosensor-assisted CRISPRi high-throughput screening to identify genetic targets in Zymomonas mobilis for high d-lactate production	6
Analyzing and engineering of the biosynthetic pathway of mollemycin A for enhancing its production	6
Enhanced chlorogenic acid production from glucose via systematic metabolic engineering of Saccharomyces cerevisiae	6
Rational construction of genome-minimized Streptomyces host for the expression of secondary metabolite gene clusters	6
Multi-modular metabolic engineering of heme synthesis in Corynebacterium glutamicum	6
Integrative analysis of transcriptome and metabolome reveals the effect of DNA methylation of chalcone isomerase gene in promoter region on Lithocarpus polystachyus Rehd flavonoids	6
Facilitating stable gene integration expression and copy number amplification in Bacillus subtilis through a reversible homologous recombination switch	6
Outside Front Cover	6
Network of nicotinamide mononucleotide biosynthesis: substrate tracking, rate-limiting enzyme, and regulatory strategy	6
Nitric oxide synthase-guided genome mining identifies a cytochrome P450 enzyme for olefin nitration in bacterial specialized metabolism	6
Artificial multi-enzyme cascades and whole-cell transformation for bioconversion of C1 compounds: Advances, challenge and perspectives	6
Constructing an artificial short route for cell-free biosynthesis of the phenethylisoquinoline scaffold	6
Efficient fatty acid synthesis from methanol in methylotrophic yeast	6
Decoding metabolic trade-offs in Halomonas elongata: Chemostat-based flux remodeling for industrial ectoine biosynthesis	6
System-level characterization of engineered and evolved formatotrophic E. coli strains	6
A two-stage metabolome refining pipeline for natural products discovery	6
Efficient secretory expression of phospholipase D for the high-yield production of phosphatidylserine and phospholipid derivates from soybean lecithin	5
Efficient stereoselective hydroxylation of deoxycholic acid by the robust whole-cell cytochrome P450 CYP107D1 biocatalyst	5
Current state and future perspectives of cytochrome P450 enzymes for C–H and C=C oxygenation	5
Identification of (-)-bornyl diphosphate synthase from Blumea balsamifera and its application for (-)-borneol biosynthesis in Saccharomyces cerevisiae	5
Polyhydroxyalkanoate production during electroactive biofilm formation and stabilization in wetland microbial fuel cells for petroleum hydrocarbon bioconversion	5
Machine learning-assisted rational design and evolution of novel signal peptides in Yarrowia lipolytica	5
Discovering the next decade's synthetic biology research trends with ChatGPT	5
Recent progress in engineering Clostridium autoethanogenum to synthesize the biochemicals and biocommodities	5
Editorial for “Biointelligent manufacturing in Memorium of Arnold (Arny) L. Demain”	5
Enhancement of polymyxin B1 production by an artificial microbial consortium of Paenibacillus polymyxa and recombinant Corynebacterium glutamicum producing precursor amino acids	5
Deletion of COM donor and acceptor domains and the interaction between modules in bacillomycin D produced by Bacillus amyloliquefaciens	5
Biosynthesis of trialkyl-substituted aromatic polyketide NFAT-133 involves unusual P450 monooxygenase-mediating aromatization and a putative metallo-beta-lactamase fold hydrolase	5
Overexpression of arginase gene CAR1 renders yeast Saccharomyces cerevisiae acetic acid tolerance	5
Expanding catalytic promiscuity of a bifunctional terpene synthase through a single mutation-induced change in hydrogen-bond network within the catalytic pocket	5
Thank you Reviewers	5
Design and mathematical analysis of activating transcriptional amplifiers that enable modular temporal control in synthetic juxtacrine circuits	5
Metabolic engineering of Escherichia coli for squalene overproduction	5
Enhancing levan biosynthesis by destroying the strongly acidic environment caused by membrane-bound glucose dehydrogenase (mGDH) in Gluconobacter sp. MP2116	5
Combined metabolic and enzymatic engineering for de novo biosynthesis of δ-tocotrienol in Yarrowia lipolytica	5
Modulating ion channels with nanobodies	5
T7 RNA polymerase-guided base editor for accelerated continuous evolution in Bacillus subtilis	5
Construction of microbial chassis for terpenoid discovery	5
Biosynthesis of Lacto-N-biose I from starch and N-acetylglucosamine via an in vitro synthetic enzymatic biosystem	5
Advances in adaptive laboratory evolution applications for Escherichia coli	5
iCASRED, a scarless DNA editing tool in E. coli for high-efficiency engineering of natural product biosynthetic gene clusters	5
Design and engineering of logic genetic-enzymatic gates based on the activity of the human CYP2C9 enzyme in permeabilized Saccharomyces cerevisiae cells	5
Efficient biosynthesis of β-caryophyllene in Saccharomyces cerevisiae by β-caryophyllene synthase from Artemisia argyi	4
Strategies to increase tolerance and robustness of industrial microorganisms	4
Biodegradation of combined pollutants of polyethylene terephthalate and phthalate esters by esterase-integrated Pseudomonas sp. JY-Q with surface-co-displayed PETase and MHETase	4
Transcriptomic studies on the product stress response revealed that YCF1 is a beneficial factor for progesterone production in Yarrowia lipolytica	4
Reconstruction and metabolic profiling of the genome-scale metabolic network model of Pseudomonas stutzeri A1501	4
Outside Front Cover	4
Engineering the cytochrome P450 to enhance parthenolide production in Saccharomyces cerevisiae	4
Mechanisms and biotechnological applications of transcription factors	4
dTSR enables efficient improvement of heterologous production of spinosad in Saccharopolyspora erythraea	4
Characterizing and engineering promoters for metabolic engineering of Ogataea polymorpha	4
The LysR family transcriptional regulator ORF-L16 regulates spinosad biosynthesis in Saccharopolyspora spinosa	4
Enzymatic properties and inhibition tolerance analysis of key enzymes in β-phenylethanol anabolic pathway of Saccharomyces cerevisiae HJ	4
Engineered microorganisms: A new direction in kidney stone prevention and treatment	4
Gene knockdown by structure defined single-stem loop small non-coding RNAs with programmable regulatory activities	4
Metabolic engineering of an industrial bacterium Zymomonas mobilis for anaerobic l-serine production	4
The potential of plant proteins as antifungal agents for agricultural applications	4
Origin and evolution of the main starch biosynthetic enzymes	4
Antibacterial activity and mechanism of X33 antimicrobial oligopeptide against Acinetobacter baumannii	4
Efficient biosynthesis of creatine by whole-cell catalysis from guanidinoacetic acid in Corynebacterium glutamicum	4
Improving solubility and copy number of taxadiene synthase to enhance the titer of taxadiene in Yarrowia lipolytica	4
Functional characterization of triterpene synthases in Cibotium barometz	4
Engineered membraneless organelles in Corynebacterium glutamicum for enhanced indigoidine biosynthesis and antimicrobial peptide production	4
Characterization of C30 carotenoid and identification of its biosynthetic gene cluster in Methylobacterium extorquens AM1	4
A rapid nucleic acid detection platform based on phosphorothioate-DNA and sulfur binding domain	4
Engineering artificial cross-species promoters with different transcriptional strengths	4
Enzyme and cofactor engineering to increase d-xylonate dehydratase activity for improved d-1,2,4-butanetriol production from d-xylose	4
Corrigendum to “Aptamer-based cell-free detection system to detect target protein” [Synth Syst Biotechnol 6 (3) (2021) 209–215]	4
Fragment antigen binding domains (Fabs) as tools to study assembly-line polyketide synthases	4
Stepwise increase of fidaxomicin in an engineered heterologous host Streptomyces albus through multi-level metabolic engineering	4
Improving cooperativity of transcription activators by oligomerization domains in mammalian cells	4
Advance of tolerance engineering on microbes for industrial production	4
Efficient production of hydroxysalidroside in Escherichia coli via enhanced glycosylation and semi-rational design of UGT85A1	4
Synthesis and assembly of full-length cyanophage A-4L genome	4
Engineering Shewanella carassii, a newly isolated exoelectrogen from activated sludge, to enhance methyl orange degradation and bioelectricity harvest	4