OOIR: Observatory of International Research

Papers

(The TQCC of Synthetic and Systems Biotechnology is 10. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-11-01 to 2025-11-01.)

Article	Citations
Promoting the production of challenging proteins via induced expression in CHO cells and modified cell-free lysates harboring T7 RNA polymerase and mutant eIF2α	85
Nobachelins, new siderophores from Nocardiopsis baichengensis protecting Caenorhabditis elegans from Pseudomonas aeruginosa infection	74
Outside Front Cover	61
A hybrid RNA-protein biosensor for high-throughput screening of adenosylcobalamin biosynthesis	61
Pangenome analysis of Enterobacteria reveals richness of secondary metabolite gene clusters and their associated gene sets	53
Parallel screening and cheminformatics modeling of flavonoid activated aptasensors	47
Metabolic engineering of Glarea lozoyensis for high-level production of pneumocandin B0	44
The biological principles and advanced applications of DSB repair in CRISPR-mediated yeast genome editing	43
Evaluation and optimization of analytical procedure and sample preparation for polar Streptomyces albus J1074 metabolome profiling	42
Quorum sensing inhibition of hordenine analogs on Pseudomonas aeruginosa and Serratia marcescens	41
Biotechnological and food synthetic biology potential of platform strain: Bacillus licheniformis	39
Degradation strategies of pesticide residue: From chemicals to synthetic biology	38
Systemically functional characterization of regiospecific flavonoid O-methyltransferases from Glycine max	35
Morphology-engineered alleviation of mycelial aggregation in Streptomyces chassis for potentiated production of secondary metabolites	35
Cost-effective hybrid long-short read assembly delineates alternative GC-rich Streptomyces hosts for natural product discovery	34
Integration of metabolic and evolutionary processes to construct efficient xylose-utilizing strain of Zymomonas mobilis for lignocellulosic ethanol production	34
DIProT: A deep learning based interactive toolkit for efficient and effective Protein design	32
Thank you Reviewers	31
Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica	30
Microbial production of cis,cis-muconic acid from aromatic compounds in engineered Pseudomonas	30
Single and multiplexed gene repression in solventogenic Clostridium via Cas12a-based CRISPR interference	29
Research progress of pathway and genome evolution in microbes	28
Achieving robust synthetic tolerance in industrial E. coli through negative auto-regulation of a DsrA-Hfq module	27
Automated characterization and analysis of expression compatibility between regulatory sequences and metabolic genes in Escherichia coli	27
WITHDRAWN: Recent Advances in the Microbial Synthesis of Human Hemoglobin	26

Synergistic Engineering PETase Reveals Loop-Region Mutations for Enhanced Catalytic Activity and Thermal Stability	26
Design and thermodynamic analysis of a pathway enabling anaerobic production of poly-3-hydroxybutyrate in Escherichia coli	26
Design and construction of microbial cell factories based on systems biology	26
Assessing the physiological properties of baker's yeast based on single-cell Raman spectrum technology	25
Characterization of putative mannoprotein in Kluyveromyces lactis for lactase production	25
CgVeA, a light signaling responsive regulator, is involved in regulation of chaetoglobosin A biosynthesis and conidia development in Chaetomium globosum	25
Enhanced depolluting capabilities of microbial bioelectrochemical systems by synthetic biology	24
Explore the dominant factor in prime editing via a view of DNA processing	23
Cofactor manipulation to drive biosynthesis of natural products	23
Engineering caveolin-mediated endocytosis in Saccharomyces cerevisiae	23
Comparative functional genomics identifies an iron-limited bottleneck in a Saccharomyces cerevisiae strain with a cytosolic-localized isobutanol pathway	23
Engineering Komagataella phaffii to biosynthesize cordycepin from methanol which drives global metabolic alterations at the transcription level	23
Engineering Yarrowia lipolytica to produce nutritional fatty acids: Current status and future perspectives	22
Surface display of carbonic anhydrase on Escherichia coli for CO2 capture and mineralization	22
Synergetic engineering of Escherichia coli for efficient production of l-tyrosine	22
Polygalacturonase-inhibiting proteins as an exogenously applied natural solution for prevention of postharvest fungal infections	21
Efficient genome editing in Claviceps purpurea using a CRISPR/Cas9 ribonucleoprotein method	21
Advances and perspectives in genetic expression and operation for the oleaginous yeast Yarrowia lipolytica	21
Synthetic biotechnology for C1 bio-refinery	21
Identification of crucial roles of transcription factor IhfA on high production of free fatty acids in Escherichia coli	20
Integrating PCR-free amplification and synergistic sensing for ultrasensitive and rapid CRISPR/Cas12a-based SARS-CoV-2 antigen detection	19
CRISPR/Cpf1–FOKI-induced gene editing in Gluconobacter oxydans	19
Analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones	19
Engineering and characterization of dehalogenase enzymes from Delftia acidovorans in bioremediation of perfluorinated compounds	19
Systematic metabolic engineering of Yarrowia lipolytica for efficient production of phytohormone abscisic acid	19
Investigation of carbonyl amidation and O-methylation during biosynthesis of the pharmacophore pyridyl of antitumor piericidins	18
Improved l-phenylglycine synthesis by introducing an engineered cofactor self-sufficient system	18
Improvement of betanin biosynthesis in Saccharomyces cerevisiae by metabolic engineering	18
Toehold switch based biosensors for sensing the highly trafficked rosewood Dalbergia maritima	18
Plant metabolism and synthetic biology	18
Glycosyltransferases: Mining, engineering and applications in biosynthesis of glycosylated plant natural products	18
Protein-protein interface analysis of the non-ribosomal peptide synthetase peptidyl carrier protein and enzymatic domains	17
Transcriptome analysis reveals methanol metabolism variations for the growth damage caused by overexpression of chimeric transactivators in Pichia pastoris	17
Development of multi-epitope mRNA vaccine against Clostridioides difficile using reverse vaccinology and immunoinformatics approaches	17
Putative functions of EpsK in teichuronic acid synthesis and phosphate starvation in Bacillus licheniformis	17
Engineering Genetic Elements for Microbial Protein Expression Systems: Advances, Challenges, Applications, and Prospects	17
Increasing prediction performance of colorectal cancer disease status using random forests classification based on metagenomic shotgun sequencing data	17
Way to efficient microbial paclitaxel mass production	16
Designing a multi-epitope vaccine against Peptostreptococcus anaerobius based on an immunoinformatics approach	16
Metabolic engineering of Bacillus amyloliquefaciens for efficient production of α-glucosidase inhibitor1-deoxynojirimycin	16
A CRISPR/Cas9-based visual toolkit enabling multiplex integration at specific genomic loci in Aspergillus niger	16
Biochemical synthesis of taxanes from mevalonate	16
A systematic discussion and comparison of the construction methods of synthetic microbial community	15
Directed evolution of the fusion enzyme for improving astaxanthin biosynthesis in Saccharomyces cerevisiae	14
Engineering Rubisco to enhance CO2 utilization	14
Engineering the thermostability of d-lyxose isomerase from Caldanaerobius polysaccharolyticus via multiple computer-aided rational design for efficient synthesis of d-mannose	14
CRISPR/Cas12a-based genome editing for cyanophage of Anabeana sp.	14
Genus-specific secondary metabolome in Allokutzneria and Kibdelosporangium	14
Applications of protein engineering in the microbial synthesis of plant triterpenoids	14
DNA storage: The future direction for medical cold data storage	13

CO2-elevated cell-free protein synthesis	13
EMT transcription factors activated circuits: A novel tool to study EMT dynamics and its therapeutic implications	13
Harnessing microbial heterogeneity for improved biosynthesis fueled by synthetic biology	13
Mechanistic insights into the orthogonal functionality of an AHL-mediated quorum-sensing circuit in Yersinia pseudotuberculosis	12
Conversion of acetate and glyoxylate to fumarate by a cell-free synthetic enzymatic biosystem	12
Synthetic biology techniques to tackle heavy metal pollution and poisoning	12
Integrated pathway engineering and transcriptome analysis for improved astaxanthin biosynthesis in Yarrowia lipolytica	12
Laccase is a multitasking protein for synthetic gene circuits in the yeast Saccharomyces cerevisiae	12
Extending dynamic and operational range of the biosensor responding to l-carnitine by directed evolution	11
Microbial synthesis of bacitracin: Recent progress, challenges, and prospects	11
Transcriptome-wide analysis of PIP reductase gene family identified a phenylpropene synthase crucial for the biosynthesis of dibenzocyclooctadiene lignans in Schisandra chinensis	11
Simulating androgen receptor selection in designer yeast	11
Coordinated regulation of two LacI family regulators, GvmR and GvmR2, on guvermectin production in Streptomyces caniferus	11
A statistical approach to enhance the productivity of Streptomyces baarensis MH-133 for bioactive compounds	11
Single carbon metabolism – A new paradigm for microbial bioprocesses?	11
Engineered microbes: Making valuable chemicals from waste gases	11
MTD: A cloud-based omics database and interactive platform for Myceliophthora thermophila	11
Development of a green Komagataella phaffii cell factory for sustainable production of plant-derived sesquiterpene (–)-α-bisabolol	11
Probing the growth and mechanical properties of Bacillus subtilis biofilms through genetic mutation strategies	11
Multi-strategy engineering unusual sugar TDP-l-mycarose biosynthesis to improve the production of 3-O-α-mycarosylerythronolide B in Escherichia coli	11
Exploration and mutagenesis of the germacrene A synthase from Solidago canadensis to enhance germacrene A production in E. coli	10
New dual functional CYP450 gene involves in isoflavone biosynthesis in Glycine max L.	10
Identification of neutral genome integration sites with high expression and high integration efficiency in Fusarium venenatum TB01	10
Developing safe and efficient CGBE editor based on Cas-embedding strategy	10
ECMpy 2.0: A Python package for automated construction and analysis of enzyme-constrained models	10
Identification, characterization, and application of an NADPH-dependent carbonyl reductase in Saccharomyces cerevisiae AS2.346 for improving production of (13R,17S)-ethyl secol	10
Biotransformation of ethylene glycol by engineered Escherichia coli	10
A flexible, modular and versatile functional part assembly toolkit for gene cluster engineering in Streptomyces	10
Construction and application of high-quality genome-scale metabolic model of Zymomonas mobilis to guide rational design of microbial cell factories	10
CRISPRi-mediated multigene downregulating redirects the metabolic flux to spinosad biosynthesis in Saccharopolyspora spinosa	10
A combined strategy for the overproduction of complex ergot alkaloid agroclavine	10
Advances in the biosynthesis of tetraacetyl phytosphingosine, a key substrate of ceramides	10