OOIR: Observatory of International Research

Papers

(The TQCC of Synthetic and Systems Biotechnology is 10. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2020-05-01 to 2024-05-01.)

Article	Citations
Microbial electro-fermentation for synthesis of chemicals and biofuels driven by bi-directional extracellular electron transfer	57
Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products	47
Discovery and modification of cytochrome P450 for plant natural products biosynthesis	46
Association between the nasopharyngeal microbiome and metabolome in patients with COVID-19	45
Challenges and advances in genome mining of ribosomally synthesized and post-translationally modified peptides (RiPPs)	42
Methodologies for preparation of prokaryotic extracts for cell-free expression systems	41
Optimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies	41
Harnessing sub-organelle metabolism for biosynthesis of isoprenoids in yeast	40
Advances in engineered Bacillus subtilis biofilms and spores, and their applications in bioremediation, biocatalysis, and biomaterials	36
Glycosyltransferases: Mining, engineering and applications in biosynthesis of glycosylated plant natural products	36
Bioengineering bacterial encapsulin nanocompartments as targeted drug delivery system	35
CRISPR base editing and prime editing: DSB and template-free editing systems for bacteria and plants	31
Screening neutral sites for metabolic engineering of methylotrophic yeast Ogataea polymorpha	30
Advances on systems metabolic engineering of Bacillus subtilis as a chassis cell	29
Emerging biosensors in detection of natural products	28
Systematic optimization of the yeast cell factory for sustainable and high efficiency production of bioactive ginsenoside compound K	27
Complex peptide natural products: Biosynthetic principles, challenges and opportunities for pathway engineering	27
Strategies to increase tolerance and robustness of industrial microorganisms	26
A high stable pH-temperature dual-sensitive liposome for tuning anticancer drug release	26
Comparison of gut microbiota in autism spectrum disorders and neurotypical boys in China: A case-control study	25
PCPD: Plant cytochrome P450 database and web-based tools for structural construction and ligand docking	24
Chemical modifications of proteins and their applications in metalloenzyme studies	24
Coordinated regulation for nature products discovery and overproduction in Streptomyces	24
Expanding application of CRISPR-Cas9 system in microorganisms	23
Melatonin biosynthesis pathways in nature and its production in engineered microorganisms	22

Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production	22
Mining and fine-tuning sugar uptake system for titer improvement of milbemycins in Streptomyces bingchenggensis	20
Rationally optimized generation of integrated Escherichia coli with stable and high yield lycopene biosynthesis from heterologous mevalonate (MVA) and lycopene expression pathways	20
Designing sgRNAs for CRISPR-BEST base editing applications with CRISPy-web 2.0	20
Surface display of carbonic anhydrase on Escherichia coli for CO2 capture and mineralization	19
Protein-protein interface analysis of the non-ribosomal peptide synthetase peptidyl carrier protein and enzymatic domains	19
Engineering Escherichia coli to improve tryptophan production via genetic manipulation of precursor and cofactor pathways	19
Biodegradation of aromatic pollutants meets synthetic biology	19
Disaccharide phosphorylases: Structure, catalytic mechanisms and directed evolution	18
Complete genome sequence of high-yield strain S. lincolnensis B48 and identification of crucial mutations contributing to lincomycin overproduction	18
Redesigning transcription factor Cre1 for alleviating carbon catabolite repression in Trichoderma reesei	17
Enhancing single-cell hyaluronic acid biosynthesis by microbial morphology engineering	16
Characterizing and engineering promoters for metabolic engineering of Ogataea polymorpha	16
Production of l-glutamate family amino acids in Corynebacterium glutamicum: Physiological mechanism, genetic modulation, and prospects	16
Integrating PCR-free amplification and synergistic sensing for ultrasensitive and rapid CRISPR/Cas12a-based SARS-CoV-2 antigen detection	16
One stone two birds: Biosynthesis of 3-hydroxypropionic acid from CO2 and syngas-derived acetic acid in Escherichia coli	16
Synthetic polycistronic sequences in eukaryotes	16
Cell-free expression of NO synthase and P450 enzyme for the biosynthesis of an unnatural amino acid L-4-nitrotryptophan	15
Using recombinant adhesive proteins as durable and green flame-retardant coatings	15
Increasing prediction performance of colorectal cancer disease status using random forests classification based on metagenomic shotgun sequencing data	15
Improving the reaction mix of a Pichia pastoris cell-free system using a design of experiments approach to minimise experimental effort	15
Antidiabetic effect of an engineered bacterium Lactobacillus plantarum-pMG36e -GLP-1 in monkey model	15
Expression of heat-resistant β-glucosidase in Escherichia coli and its application in the production of gardenia blue	14
Modular engineering of E. coli coculture for efficient production of resveratrol from glucose and arabinose mixture	13
Genome-wide analysis of UGT gene family identified key gene for the biosynthesis of bioactive flavonol glycosides in Epimedium pubescens Maxim.	13
Engineering and characterization of dehalogenase enzymes from Delftia acidovorans in bioremediation of perfluorinated compounds	13
Site directed mutagenesis as a precision tool to enable synthetic biology with engineered modular polyketide synthases	13
Production of (2S)-sakuranetin from (2S)-naringenin in Escherichia coli by strengthening methylation process and cell resistance	12
Construction of synthetic microbial consortia for 2-keto-L-gulonic acid biosynthesis	12
Comparative proteomics analysis of Pichia pastoris cultivating in glucose and methanol	12
Metabolic engineering of Yarrowia lipolytica for scutellarin production	12
Design and construction of microbial cell factories based on systems biology	12
An aurora of natural products-based drug discovery is coming	11
Positional assembly of multi-enzyme cascade reaction in polyelectrolyte doped microcapsule through electrospray and layer-by-layer assembly	11
Enrichment of health-promoting lutein and zeaxanthin in tomato fruit through metabolic engineering	11
A CRISPR/Cas9-based single-stranded DNA recombineering system for genome editing of Rhodococcus opacus PD630	11
Degradation strategies of pesticide residue: From chemicals to synthetic biology	11
Comparative metagenomic analysis of the vaginal microbiome in healthy women	11
Discovering the next decade's synthetic biology research trends with ChatGPT	11
A CRISPRi mediated self-inducible system for dynamic regulation of TCA cycle and improvement of itaconic acid production in Escherichia coli	11
Multiscale engineering of microbial cell factories: A step forward towards sustainable natural products industry	11
Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs	11
Complex natural product production methods and options	11
Directed evolution of the fusion enzyme for improving astaxanthin biosynthesis in Saccharomyces cerevisiae	10
Engineering Shewanella carassii, a newly isolated exoelectrogen from activated sludge, to enhance methyl orange degradation and bioelectricity harvest	10
Pangenome analysis of Enterobacteria reveals richness of secondary metabolite gene clusters and their associated gene sets	10
Screening and engineering of high-activity promoter elements through transcriptomics and red fluorescent protein visualization in Rhodobacter sphaeroides	10
Exploring the catalytic function and active sites of a novel C-glycosyltransferase from Anemarrhena asphodeloides	10
O-glycosyltransferases from Homo sapiens contributes to the biosynthesis of Glycyrrhetic Acid 3-O-mono-β-D-glucuronide and Glycyrrhizin in Saccharomyces cerevisiae	10
The potential of plant proteins as antifungal agents for agricultural applications	10

Aptamer-based cell-free detection system to detect target protein	10
Current state and future perspectives of cytochrome P450 enzymes for C–H and C=C oxygenation	10
Increasing the heterologous production of spinosad in Streptomyces albus J1074 by regulating biosynthesis of its polyketide skeleton	10
Converting Escherichia coli MG1655 into a chemical overproducer through inactivating defense system against exogenous DNA	10