OOIR: Observatory of International Research

Papers

(The median citation count of CRISPR Journal is 2. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2022-08-01 to 2026-08-01.)

Article	Citations
Gene Editing Corrects In Vitro a G > A GLB1 Transition from a GM1 Gangliosidosis Patient	51
Exploring the Cytoplasmic Retention of CRISPR-Cas9 in Eukaryotic Cells: The Role of Nuclear Localization Signals and Ribosomal Interactions	39
Will Advances in Genetic Medicine Lessen the Need for Germline Modifications?	37
Increasing Genome Editing Efficiency of Cas9 Nucleases by the Simultaneous Use of Transcriptional Activators and Histone Acetyltransferase Activator	36
Adaptation by Type V-A and V-B CRISPR-Cas Systems Demonstrates Conserved Protospacer Selection Mechanisms Between Diverse CRISPR-Cas Types	33
CRISPR Comparison Toolkit: Rapid Identification, Visualization, and Analysis of CRISPR Array Diversity	31
Subtypes of Type I-E CRISPR-Cas Systems Distribution in Human Escherichia coli Isolates from China	31
Hairpin Internal Nuclear Localization Signals in CRISPR-Cas9 Enhance Editing in Primary Human Lymphocytes	30
Gene Editing and Rett Syndrome: Does It Make the Cut?	24
Acknowledgment of Reviewers 2022	23
Opposing Human Genetic Engineering	21
The Lexicon of CRISPR: When Is It Too Much?	19
Advances in Cas12a-Based Amplification-Free Nucleic Acid Detection	19
Novel CRISPR-Associated Gene-Editing Systems Discovered in Metagenomic Samples Enable Efficient and Specific Genome Engineering	18
Curing “GFP-itis” in Bacteria with Base Editors: Development of a Genome Editing Science Program Implemented with High School Biology Students	17
Generation of a Commercial-Scale Founder Population of Porcine Reproductive and Respiratory Syndrome Virus Resistant Pigs Using CRISPR-Cas	17
Early Detection of Wildlife Disease Pathogens Using CRISPR-Cas System Methods	15
Efficient Installation of Heterozygous Mutations in Human Pluripotent Stem Cells Using Prime Editing	15
CRISPR Crops and Sustainable Agriculture	14
The Roles and Responsibilities of Scientific Journals in Research Governance: Editorial Policymaking at Nature and Springer Nature (1995–2023)	14
Viability-Based Assessment Reveals True Efficiency of CRISPR-Cas9 Transfection Methods in Rabbit Spermatozoa	14
Generation of Human Isogenic Induced Pluripotent Stem Cell Lines with CRISPR Prime Editing	14
CRISPR-Cas9 Single Nucleotide Editing of Tuberous Sclerosis Complex 2 Gene in Mesenchymal Stem Cells	13
Efficient Homology-Directed Repair with Circular Single-Stranded DNA Donors	13
Regulatory Support for CRISPR in Ag: A Timely EU-Turn	13

Comprehensive Analysis of CRISPR-Cas9 Editing Outcomes in Yeast Xanthophyllomyces dendrorhous	13
Widespread Impact of Natural Genetic Variations in CRISPR-Cas9 Outcomes	12
dCas9 Tells Tales: Probing Gene Function and Transcription Regulation in Cancer	12
Automated Good Manufacturing Practice-Compatible CRISPR-Cas9 Editing of Hematopoietic Stem and Progenitor Cells for Clinical Treatment of β-Hemoglobinopathies	12
Engineering CjCas9 for Efficient Base Editing and Prime Editing	12
Institutional Bioethics: From Critical Interdisciplinarity to Cosmopolitanism	12
A Story of Perseverance: An Interview with Matthew Porteus	12
Specific High-Sensitivity Enzymatic Reporter UnLOCKing-Mediated Detection of Oncogenic BCR::ABL1 and EGFR	12
Gene Editing in the Chagas Disease Vector Rhodnius prolixus by Cas9-Mediated ReMOT Control	11
Enhancements of the CRISPR-Cas System in the Silkworm Bombyx mori	11
Fanzors: Mysterious TnpB-Like Bacterial Transposon-Related RNA-Guided DNA Nucleases of Eukaryotes	11
CRISPR Momentum in the Clinic and the Field	11
Engineered Antiviral Sensor Targets Infected Mosquitoes	11
Efficient CRISPR-Cas9-Mediated Genome Editing of the Cane Toad ( Rhinella marina )	11
Affordable Pricing of CRISPR Treatments is a Pressing Ethical Imperative	10
Conditional Expression of Cas9 and dCas9 in Lucilia cuprina Reveals dCas9-Associated Lethality	10
A Multiplexed Cas13-Based Assay with Point-of-Care Attributes for Simultaneous COVID-19 Diagnosis and Variant Surveillance	10
A Fluorescent Reporter Mouse for In Vivo Assessment of Genome Editing with Diverse Cas Nucleases and Prime Editors	10
A PCR-Induced Mutagenesis-Restriction Fragment Length Polymorphism Method for the Detection of CRISPR-Induced Indels	10
Are We Making Genetically Modified Humans?	9
Rapid and Technically Simple Detection of SARS-CoV-2 Variants Using CRISPR Cas12 and Cas13	9
Cas12a-Capture: A Novel, Low-Cost, and Scalable Method for Targeted Sequencing	9
Navigating Viral Space with CRISPR Technologies	9
Warrior Spirit: An Interview with Victoria Gray, Sickle Cell Pioneer	9
Introducing Faith-Based Claims to Ethical Discourse on Scientific Matters	9
CRISPR-Cas9-Mediated Correction of TSC2 Pathogenic Variants in iPSCs from Patients with Tuberous Sclerosis Complex Type 2	9
The CRISPR Revolution in the Drug Discovery Workflow: An Industry Perspective	9
A Protein-Cutting CRISPR Complex Caught in Action	9
Genome Editing Therapy for the Blood: Ex Vivo Success and In Vivo Prospects	8
Acknowledgment of Reviewers 2023	8
Five Years of Progress in CRISPR Clinical Trials (2019–2024)	8
Rapid Variant Pathogenicity Analysis by CRISPR Activation of CRB1 Gene Expression in Patient-Derived Fibroblasts	8
CRISPR Medicine Needs Infrastructure, Not Just Innovation	8
An Optimized Workflow for In Vitro Transcription of Single Guide RNAs Minimizes Innate Immune Activation	7
Moving Therapeutic Genome Editing into Global Clinical Trials and Medicine	7
Trichostatin A for Efficient CRISPR-Cas9 Gene Editing of Human Pluripotent Stem Cells	7
Strategies for Interdisciplinary Human Gene Editing Research: Insights from a Swiss Project	7
Genome Editing Headwinds: Can CRISPR Stay on Target?	7
The New Old Colonialism	7
Correction to: APOBEC Reporter Systems for Evaluating diNucleotide Editing Levels by Rieffer et al. The CRISPR Journal	6
Diverse Class 2 CRISPR Effectors as Active Nucleases with Expanded Targeting Capabilities	6
Recalibrating Health Governance, Equity, and Reproductive Rights in India: A Case Study from the Global South	6
Novel off-Targeting Events Identified after Genome Wide Analysis of CRISPR-Cas Edited Pigs	6
Special Issue : Manipulating the Microbiome with CRISPR	6
Bio-Orthogonal Chemistry Conjugation Strategy Facilitates Investigation of N-methyladenosine and Thiouridine Guide RNA Modifications on CRISPR Activity	6
Extreme Genome Editing	6
Correction to: Give Cas a Chance: An Actionable Path to a Platform for CRISPR Cures , by Fyodor D. Urnov [DOI: 10.1089/crispr.2024.0082]	6
Characterization of Research Support of Genome Editing Technologies and Transition to Clinical Trials	6
Development and Characterization of RNA Aptamer-Mediated Modular Base Editors Containing Staphylococcus aureus Cas9 Derivatives and Novel Deaminase Orthologs	6
Special Issue : CRISPR Trials	6

A Reset for Bioethics: A Statement from the Global Observatory for Genome Editing	6
Cosmopolitan Bioethics Under Pressure: Reflections on the Global Observatory for Genome Editing International Summit	5
Clonally Selected Lines After CRISPR-Cas Editing Are Not Isogenic	5
Optimization of Genome-Wide CRISPR Screens Using Dual-Guide RNA Infection with Cas9 Electroporation (DICE)	5
CRISPR-Cas9 Long-Read Sequencing for Mapping Transgenes in the Mouse Genome	5
An Efficient and Cost-Effective Novel Strategy for Identifying CRISPR-Cas-Mediated Mutants in Plant Offspring	5
CRISPR-Cas9-Mediated Targeting of Multidrug Resistance Genes in Methicillin-Resistant Staphylococcus aureus	5
PASTE: The Way Forward for Large DNA Insertions	5
Introducing Perspectives from the Global Observatory for Genome Editing	4
Modified Cas9-Guided Oxford Nanopore Technology Sequencing Uncovers Single and Multiple Transgene Insertion Sites in a Zebrafish Melanoma Model	4
Correction to: Genotyping MUltiplexed-Sequencing of CRISPR-Localized Editing (GMUSCLE): An Experimental and Computational Approach for Analyzing CRISPR-Edited Cells	4
A Phage Variable Region Encodes Anti-CRISPR Proteins Inhibiting All Streptococcus thermophilus CRISPR Immune Systems	4
Viral Delivery of Compact CRISPR-Cas12f for Gene Editing Applications	4
Optimization of Genomewide CRISPR Screens Using AsCas12a and Multi-Guide Arrays	4
Facing Systemic Uncertainty, Can the CRISPR Community Stay the Course?	4
Novel and Engineered Type II CRISPR Systems from Uncultivated Microbes with Broad Genome Editing Capability	4
BAR-CAT: Targeted Recovery of Synthetic Genes via Barcode-Directed CRISPR-dCas9 Enrichment	4
Reprogramming of Human B Cells from Secreting IgG to IgM by Genome Editing	4
Science with Society at Science Gallery Bengaluru	4
APOBEC Reporter Systems for Evaluating diNucleotide Editing Levels	4
The Diversifying Distribution Trends of Maturing CRISPR Technologies by Addgene	4
Toward a Global Deliberative System for Genome Editing	3
Response to Cook et al. re: Novel Off-Targeting Events Identified After Genome Wide Analysis of CRISPR-Cas Edited Pig	3
A “Bare Hope of A Result”: The Second CRISPR Patent Appeal	3
CRISPR-Cas9 Genome Editing Uncovers the Mode of Action of Methoprene in the Yellow Fever Mosquito, Aedes aegypti	3
Identification of a Guide RNA Targeting an Ultraconserved Element for Evaluation of Cas9 Genome Editors Across Mammalian Species	3
Enhanced Myogenesis by Silencing Myostatin with Nonviral Delivery of a dCas9 Ribonucleoprotein Complex	3
Predicting Mutations Generated by Cas9, Base Editing, and Prime Editing in Mammalian Cells	3
CRISPRi-Mediated Treatment of Dominant Rhodopsin-Associated Retinitis Pigmentosa	3
Developing Bottom-Up Induced Pluripotent Stem Cell Derived Solid Tumor Models Using Precision Genome Editing Technologies	3
AI and SynBio Meet CRISPR Heralding a New Genome Editing Era	3
Highly Efficient One-Step Tagging of Endogenous Genes in Primary Cells Using CRISPR-Cas Ribonucleoproteins	3
Discovery and Characterization of Novel Type V Cas12f Nucleases with Diverse Protospacer Adjacent Motif Preferences	3
Genome Editing in Apicomplexan Parasites: Current Status, Challenges, and Future Possibilities	3
Modulating CRISPR-Cas Genome Editing Using Guide-Complementary DNA Oligonucleotides	3
Biotechnologies in the World: On Global Asymmetries and the Need for Cosmopolitanism	3
The Promises and Pitfalls of CRISPR-Mediated Base Editing in Stem Cells	3
Bacterial Expression System with Deep Repression and Activation via CRISPR-Cas9	2
Enrichment of Allelic Editing Outcomes by Prime Editing in Induced Pluripotent Stem Cells	2
Induction of SpCas9-Directed Immune Responses Using Lipid Nanoparticles and Identification of SpCas9-Derived T Cell Epitopes in C57BL/6 Mice	2
CRISPR Empowers Tree Bioengineering for a Sustainable Future	2
Establishment of a CRISPR-Cas9-Mediated Genome Editing System in Flax	2
CRISPR and the Plant Pathologists' Holy Grail	2
Efficient Generation of SOCS2 Knock-Out Sheep by Electroporation of CRISPR-Cas9 Ribonucleoprotein Complex with Dual-sgRNAs	2
JACKIE: Fast Enumeration of Genome-Wide Single- and Multicopy CRISPR Target Sites and Their Off-Target Numbers	2
Advances in Gene Therapy for Sickle Cell Disease: From Preclinical Innovations to Clinical Implementation and Access Challenges	2
Monitoring the Land and Sea: Enhancing Efficiency Through CRISPR-Cas Driven Depletion and Enrichment of Environmental DNA	2
Allele-Specific Inactivation of an Autosomal Dominant Epidermolysis Bullosa Simplex Mutation Using CRISPR-Cas9	2
Model System to Analyze RNA-Mediated DNA Repair in Mammalian Cells	2
Optimizing Genome Editing in Mollusks ( Crassostrea gigas ) in Vitro Validation of sgRNA and Identifying K	2
Correction to: Generation of a Commercial-Scale Founder Population of Porcine Reproductive and Respiratory Syndrome Virus Resistant Pigs Using CRISPR-Cas	2
Special Issue : Manipulating the Microbiome with CRISPR	2
Periodontal Disease Pathogens, Pathogenesis, and Therapeutics: The CRISPR-Cas Effect	2
Rosalind Franklin Society Proudly Announces the 2023 Award Recipient for The CRISPR Journal	2