OOIR: Observatory of International Research

Papers

(The median citation count of CRISPR Journal is 2. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-11-01 to 2025-11-01.)

Article	Citations
Will Advances in Genetic Medicine Lessen the Need for Germline Modifications?	508
Exploring the Cytoplasmic Retention of CRISPR-Cas9 in Eukaryotic Cells: The Role of Nuclear Localization Signals and Ribosomal Interactions	52
Adaptation by Type V-A and V-B CRISPR-Cas Systems Demonstrates Conserved Protospacer Selection Mechanisms Between Diverse CRISPR-Cas Types	51
Increasing Genome Editing Efficiency of Cas9 Nucleases by the Simultaneous Use of Transcriptional Activators and Histone Acetyltransferase Activator	42
Hairpin Internal Nuclear Localization Signals in CRISPR-Cas9 Enhance Editing in Primary Human Lymphocytes	41
Gene Editing Corrects In Vitro a G > A GLB1 Transition from a GM1 Gangliosidosis Patient	40
CRISPR Comparison Toolkit: Rapid Identification, Visualization, and Analysis of CRISPR Array Diversity	38
Inference of CRISPR Edits from Sanger Trace Data	33
A Taxonomic and Phylogenetic Classification of Diverse Base Editors	32
Gene Editing and Rett Syndrome: Does It Make the Cut?	32
Opposing Human Genetic Engineering	30
Curing “GFP-itis” in Bacteria with Base Editors: Development of a Genome Editing Science Program Implemented with High School Biology Students	29
Advances in Cas12a-Based Amplification-Free Nucleic Acid Detection	28
Acknowledgment of Reviewers 2022	28
Novel CRISPR-Associated Gene-Editing Systems Discovered in Metagenomic Samples Enable Efficient and Specific Genome Engineering	24
Generation of a Commercial-Scale Founder Population of Porcine Reproductive and Respiratory Syndrome Virus Resistant Pigs Using CRISPR-Cas	24
The Lexicon of CRISPR: When Is It Too Much?	23
Early Detection of Wildlife Disease Pathogens Using CRISPR-Cas System Methods	22
Efficient Installation of Heterozygous Mutations in Human Pluripotent Stem Cells Using Prime Editing	22
Increasing the Targeting Scope of CRISPR Base Editing System Beyond NGG	21
The Roles and Responsibilities of Scientific Journals in Research Governance: Editorial Policymaking at Nature and Springer Nature (1995–2023)	18
CRISPR Crops and Sustainable Agriculture	18
Implementing CRISPR-Cas9 Yeast Practicals into Biology Curricula	18
Generation of Human Isogenic Induced Pluripotent Stem Cell Lines with CRISPR Prime Editing	17
Comprehensive Analysis of CRISPR-Cas9 Editing Outcomes in Yeast Xanthophyllomyces dendrorhous	17

Efficient Homology-Directed Repair with Circular Single-Stranded DNA Donors	16
CRISPR Rewrites the Future of Medicine	15
CRISPR à la carte	15
A Story of Perseverance: An Interview with Matthew Porteus	14
Specific High-Sensitivity Enzymatic Reporter UnLOCKing-Mediated Detection of Oncogenic BCR::ABL1 and EGFR Rearrangements	13
dCas9 Tells Tales: Probing Gene Function and Transcription Regulation in Cancer	13
CRISPR-Cas9 Single Nucleotide Editing of Tuberous Sclerosis Complex 2 Gene in Mesenchymal Stem Cells	13
Engineering CjCas9 for Efficient Base Editing and Prime Editing	13
Institutional Bioethics: From Critical Interdisciplinarity to Cosmopolitanism	12
Automated Good Manufacturing Practice-Compatible CRISPR-Cas9 Editing of Hematopoietic Stem and Progenitor Cells for Clinical Treatment of β-Hemoglobinopathies	12
Widespread Impact of Natural Genetic Variations in CRISPR-Cas9 Outcomes	12
Engineered Antiviral Sensor Targets Infected Mosquitoes	11
Rational Engineering of CRISPR-Cas9 Nuclease to Attenuate Position-Dependent Off-Target Effects	11
Evolutionary Biology and Gene Editing of Cat Allergen, Fel d 1	11
Combined Theoretical, Bioinformatic, and Biochemical Analyses of RNA Editing by Adenine Base Editors	11
CRISPR Momentum in the Clinic and the Field	11
Gene Editing in the Chagas Disease Vector Rhodnius prolixus by Cas9-Mediated ReMOT Control	11
Enhancements of the CRISPR-Cas System in the Silkworm Bombyx mori	10
A PCR-Induced Mutagenesis-Restriction Fragment Length Polymorphism Method for the Detection of CRISPR-Induced Indels	10
Affordable Pricing of CRISPR Treatments is a Pressing Ethical Imperative	10
A Multiplexed Cas13-Based Assay with Point-of-Care Attributes for Simultaneous COVID-19 Diagnosis and Variant Surveillance	10
Efficient CRISPR-Cas9-Mediated Genome Editing of the Cane Toad (Rhinella marina)	10
A Fluorescent Reporter Mouse for In Vivo Assessment of Genome Editing with Diverse Cas Nucleases and Prime Editors	10
Cas12a-Capture: A Novel, Low-Cost, and Scalable Method for Targeted Sequencing	9
The CRISPR Revolution in the Drug Discovery Workflow: An Industry Perspective	9
Fanzors: Mysterious TnpB-Like Bacterial Transposon-Related RNA-Guided DNA Nucleases of Eukaryotes	9
Warrior Spirit: An Interview with Victoria Gray, Sickle Cell Pioneer	9
A Protein-Cutting CRISPR Complex Caught in Action	9
It's a Knockout for Cat Allergies?	9
Introducing Faith-Based Claims to Ethical Discourse on Scientific Matters	8
Bacterial Retrons Enable Precise Gene Editing in Human Cells	8
Rapid and Technically Simple Detection of SARS-CoV-2 Variants Using CRISPR Cas12 and Cas13	8
Five Years of Progress in CRISPR Clinical Trials (2019–2024)	7
Acknowledgment of Reviewers 2023	7
CRISPR-Cas9 Gene Editing Protects from the A53T-SNCA Overexpression-Induced Pathology of Parkinson's Disease In Vivo	7
Moving Therapeutic Genome Editing into Global Clinical Trials and Medicine	7
Reduction of Pre-Existing Adaptive Immune Responses Against SaCas9 in Humans Using Epitope Mapping and Identification	7
CRISPR-Cas9-Mediated Correction of TSC2 Pathogenic Variants in iPSCs from Patients with Tuberous Sclerosis Complex Type 2	7
Genome Editing Therapy for the Blood: Ex Vivo Success and In Vivo Prospects	7
Genome Editing Headwinds: Can CRISPR Stay on Target?	7
Trichostatin A for Efficient CRISPR-Cas9 Gene Editing of Human Pluripotent Stem Cells	7
Navigating Viral Space with CRISPR Technologies	7
Rapid Variant Pathogenicity Analysis by CRISPR Activation of CRB1 Gene Expression in Patient-Derived Fibroblasts	7
The New Old Colonialism	7
Base Editing of Somatic Cells Using CRISPR–Cas9 in Drosophila	7
Special Issue: Manipulating the Microbiome with CRISPR	6
Bio-Orthogonal Chemistry Conjugation Strategy Facilitates Investigation of N-methyladenosine and Thiouridine Guide RNA Modifications on CRISPR Activity	6
Novel off-Targeting Events Identified after Genome Wide Analysis of CRISPR-Cas Edited Pigs	6
Correction to: APOBEC Reporter Systems for Evaluating diNucleotide Editing Levels by Rieffer et al. The CRISPR Journal, 2023;6(5):430–446; DOI: 10.1089/crispr.2023.0027	6
Diverse Class 2 CRISPR Effectors as Active Nucleases with Expanded Targeting Capabilities	6

Correction to: Give Cas a Chance: An Actionable Path to a Platform for CRISPR Cures, by Fyodor D. Urnov [DOI: 10.1089/crispr.2024.0082]	6
Characterization of Research Support of Genome Editing Technologies and Transition to Clinical Trials	6
Special Issue: CRISPR Trials	6
Extreme Genome Editing	6
PASTE: The Way Forward for Large DNA Insertions	5
A Reset for Bioethics: A Statement from the Global Observatory for Genome Editing	5
An Efficient and Cost-Effective Novel Strategy for Identifying CRISPR-Cas-Mediated Mutants in Plant Offspring	5
Reprogramming of Human B Cells from Secreting IgG to IgM by Genome Editing	5
Celebrating a Decade of CRISPR-Fueled Genome Editing	5
CRISPR-Cas9-Mediated Targeting of Multidrug Resistance Genes in Methicillin-Resistant Staphylococcus aureus	5
Electroporation-Mediated Delivery of Cas9 Ribonucleoproteins Results in High Levels of Gene Editing in Primary Hepatocytes	5
CRISPR-Cas9 Long-Read Sequencing for Mapping Transgenes in the Mouse Genome	5
Clonally Selected Lines After CRISPR-Cas Editing Are Not Isogenic	5
Recalibrating Health Governance, Equity, and Reproductive Rights in India: A Case Study from the Global South	5
Facing Systemic Uncertainty, Can the CRISPR Community Stay the Course?	5
Introducing Perspectives from the Global Observatory for Genome Editing	4
A Phage Variable Region Encodes Anti-CRISPR Proteins Inhibiting All Streptococcus thermophilus CRISPR Immune Systems	4
Correction to: Genotyping MUltiplexed-Sequencing of CRISPR-Localized Editing (GMUSCLE): An Experimental and Computational Approach for Analyzing CRISPR-Edited Cells by Zhang et al. The CRISP	4
Green Fluorescent Protein Tagged Polycistronic Reporter System Reveals Functional Editing Characteristics of CRISPR-Cas	4
Discovery and Characterization of Novel Type V Cas12f Nucleases with Diverse Protospacer Adjacent Motif Preferences	4
Enhanced Myogenesis by Silencing Myostatin with Nonviral Delivery of a dCas9 Ribonucleoprotein Complex	4
APOBEC Reporter Systems for Evaluating diNucleotide Editing Levels	4
Viral Delivery of Compact CRISPR-Cas12f for Gene Editing Applications	4
Science with Society at Science Gallery Bengaluru	4
Predicting Mutations Generated by Cas9, Base Editing, and Prime Editing in Mammalian Cells	4
Toward a Global Deliberative System for Genome Editing	4
Novel and Engineered Type II CRISPR Systems from Uncultivated Microbes with Broad Genome Editing Capability	4
Optimization of Genomewide CRISPR Screens Using AsCas12a and Multi-Guide Arrays	4
Modified Cas9-Guided Oxford Nanopore Technology Sequencing Uncovers Single and Multiple Transgene Insertion Sites in a Zebrafish Melanoma Model	4
Genetic Manipulation of a CAST of Characters in a Microbial Community	4
High-Throughput Imaging of CRISPR- and Recombinant Adeno-Associated Virus–Induced DNA Damage Response in Human Hematopoietic Stem and Progenitor Cells	4
Response to Cook et al. re: Novel Off-Targeting Events Identified After Genome Wide Analysis of CRISPR-Cas Edited Pig	4
CRISPRi-Mediated Treatment of Dominant Rhodopsin-Associated Retinitis Pigmentosa	4
Genomic and Transcriptomic Analyses of Prime Editing Guide RNA–Independent Off-Target Effects by Prime Editors	3
Identification of a Guide RNA Targeting an Ultraconserved Element for Evaluation of Cas9 Genome Editors Across Mammalian Species	3
AI and SynBio Meet CRISPR Heralding a New Genome Editing Era	3
A “Bare Hope of A Result”: The Second CRISPR Patent Appeal	3
Acknowledgment of Reviewers 2021	3
Correction to: Generation of a Commercial-Scale Founder Population of Porcine Reproductive and Respiratory Syndrome Virus Resistant Pigs Using CRISPR-Cas by Burger et al. The CRISPR Journal,	3
CRISPR-Cas9 Genome Editing Uncovers the Mode of Action of Methoprene in the Yellow Fever Mosquito, Aedes aegypti	3
Genome Editing in Apicomplexan Parasites: Current Status, Challenges, and Future Possibilities	3
Special Issue on CRISPR Diagnostics	3
Modulating CRISPR-Cas Genome Editing Using Guide-Complementary DNA Oligonucleotides	3
Developing Bottom-Up Induced Pluripotent Stem Cell Derived Solid Tumor Models Using Precision Genome Editing Technologies	3
CRISPR Empowers Tree Bioengineering for a Sustainable Future	2
The Promises and Pitfalls of CRISPR-Mediated Base Editing in Stem Cells	2
Compact Cje3Cas9 for Efficient In Vivo Genome Editing and Adenine Base Editing	2
Establishment of a CRISPR-Cas9-Mediated Genome Editing System in Flax	2
Optimizing Genome Editing in Mollusks (Crassostrea gigas) in Vitro Validation of sgRNA and Identifying Key Factors Influencing Efficiency	2
Rosalind Franklin Society Proudly Announces the 2023 Award Recipient for The CRISPR Journal	2
Search for Origins of Anti-CRISPR Proteins by Structure Comparison	2
Advances in Gene Therapy for Sickle Cell Disease: From Preclinical Innovations to Clinical Implementation and Access Challenges	2
JACKIE: Fast Enumeration of Genome-Wide Single- and Multicopy CRISPR Target Sites and Their Off-Target Numbers	2
Periodontal Disease Pathogens, Pathogenesis, and Therapeutics: The CRISPR-Cas Effect	2
Monitoring the Land and Sea: Enhancing Efficiency Through CRISPR-Cas Driven Depletion and Enrichment of Environmental DNA	2
Model System to Analyze RNA-Mediated DNA Repair in Mammalian Cells	2
Functional and Phylogenetic Diversity of Cas10 Proteins	2
Allele-Specific Inactivation of an Autosomal Dominant Epidermolysis Bullosa Simplex Mutation Using CRISPR-Cas9	2
Special Issue: Manipulating the Microbiome with CRISPR	2
Highly Efficient One-Step Tagging of Endogenous Genes in Primary Cells Using CRISPR-Cas Ribonucleoproteins	2
Biotechnologies in the World: On Global Asymmetries and the Need for Cosmopolitanism	2
CRISPR's Path to the Clinic	2
Enrichment of Allelic Editing Outcomes by Prime Editing in Induced Pluripotent Stem Cells	2
CRISPR and the Plant Pathologists' Holy Grail	2
A Mutation-Agnostic Hematopoietic Stem Cell Gene Therapy for Metachromatic Leukodystrophy	2