Biotechnology Progress

Article	Citations
Characterization of large transgene integrations in Chinese hamster ovary cells using a bioengineered mammalian transposase	85
Development of targeted gene delivery system based on liposome and PAMAM dendrimer functionalized with hyaluronic acid and TAT peptide: In vitro and in vivo studies	65
Characterization of soy protein hydrolysates and influence of its iron content on monoclonal antibody production by a murine hybridoma cell line	58
Issue Information	32
Optimization of carotenoid production by Umbelopsis ramanniana	32
The pandemic and resilience for the future: AccBio 2021	30
Modulating and optimizing Pluronic F‐68 concentrations and feeding for intensified perfusion Chinese hamster ovary cell cultures	26
Valorizing prickly pear cladodes via methane‐arrested anaerobic digestion for carboxylic acid production	24
Acetate accumulation and regulation by process parameters control in Chinese hamster ovary cell culture	24
Issue Information	24
Methods comparison of two‐dimensional gel electrophoresis for host cell protein characterization	23
Scale‐up issues during sterile filtration of glycoconjugate vaccines	21
Issue Information	21
Relationship between intracellular protein denaturation and irreversible inactivation of Saccharomyces pastorianus by low‐pressure carbon dioxide microbubbles	19
Establishment and characterization of lung co‐culture spheroids for paclitaxel loaded Eudragit® RL 100 nanoparticle evaluation	18
Surfactants reduce aggregation of monoclonal antibodies in cell culture medium with improvement in performance of mammalian cell culture	17
Optimization and characterization of rhamnolipid production by Pseudomonas aeruginosa NY3 using waste frying oil as	16
Protective effects of non‐catalytic proteins on endoglucanase activity at air and lignin interfaces	15
Retrospective assessment of clonality of a legacy cell line by analytical subcloning of the master cell bank	14
Assessment of corn stover pretreated with shock and alkali using methane‐arrested anaerobic digestion	14
Assessing the impact of sanitization methods for regenerated cellulose ultrafiltration/diafiltration membrane on membrane integrity and protein quality	13
Online monitoring of the respiration activity in 96‐deep‐well microtiter plate Chinese hamster ovary cultures streamlines kill curve experiments	13
Genetic rearrangement during site specific integration event facilitates cell line development of a bispecific molecule	13
A novel synthetic sRNA promoting protein overexpression in cell‐free systems	13
X‐ray sterilization of biopharmaceutical manufacturing equipment—Extractables profile of a film material and copolyester Tritan™ compared to gamma irradiation	13

Real‐time online monitoring of insect cell proliferation and baculovirus infection using digital differential holographic microscopy and machine learning	13
Deep brain stimulation: Challenges at the tissue‐electrode interface and current solutions	12
Cation exchange as a single polishing step for conjugated peptides	12
Development of a pan‐tau multivalent nanobody that binds tau aggregation motifs and recognizes pathological tau aggregates	12
New technical concept for alternating tangential flow filtration in biotechnological cell separation processes	12
Multivariate data analysis of process parameters affecting the growth and productivity of stable Chinese hamster ovary cell pools expressing SARS‐CoV‐2 spike protein as vaccine antigen in e	12
CHO stable pool fed‐batch process development of SARS‐CoV‐2 spike protein production: Impact of aeration conditions and feeding strategies	11
High‐yield recombinant adeno‐associated viral vector production by multivariate optimization of bioprocess and transfection conditions	11
From protein structure to an optimized chromatographic capture step using multiscale modeling	11
Hybrid modeling for in silico optimization of a dynamic perfusion cell culture process	11
Dynamic flux balance analysis of 1,3‐propanediol production by clostridium butyricum fermentation	11
Recent advances of dendrimer in targeted delivery of drugs and genes to stem cells as cellular vehicles	10
Effect of iron addition on mAb productivity and oxidative stress in Chinese hamster ovary culture	10
Depth filtration for clarification of intensified lentiviral vector suspension cell culture	10
Optimized pulse‐feeding fed‐batch fermentation for enhanced lignin to polyhydroxyalkanoate transformation	10
Detergent/surfactant retention during ultrafiltration in the formulation of biotherapeutics	10
Proteomics‐based method to comprehensively model the removal of host cell protein impurities	9
Chemistry of lignin and hemicellulose structures interacts with hydrothermal pretreatment severity and affects cellulose conversion	9
A comparison of different intensified upstream processes highlighting the advantage of WuXi Biologics' Ultra‐high Productivity platform (WuXiUPTM) in improved product quality and	9
Predictive modeling of single pass tangential flow filtration for continuous biomanufacturing	8
Activation of the PERK branch of the unfolded protein response during production reduces specific productivity in CHO cells via downregulation of PDGFRa and IRE1a	8
Evaluation of the efficiency of modified PAMAM dendrimer with low molecular weight protamine peptide to deliver IL ‐12 plasmid int	8
Exometabolome profiling reveals activation of the carnitine buffering pathway in fed‐batch cultures ofCHOcells co‐fed with glucose and lactic acid	8
Online measurement of the viscosity in shake flasks enables monitoring of γ‐PGA production in depolymerase knockout mutants of Bacillus subtilis with the phosphate‐starvation inducib	8
Metabolic trends of Chinese hamster ovary cells in biopharmaceutical production under batch and fed‐batch conditions	8
A Novel Polyculture Growth Model of Native Microalgal Communities to Estimate Biomass Productivity for Biofuel Production	8
Mesenchymal stem cells derived‐exosomes enhanced amniotic membrane extract promotes corneal keratocyte proliferation	8
Model validation and selection in metabolic flux analysis and flux balance analysis	8
An adaptive modeling approach using spiking‐augmentation method to improve chemometric model performance in bioprocess monitoring	8
Bio‐based composite granules with simultaneous biocontrol and phosphorus fertilization roles: Outcomes from a lab‐scale in vitro assessment	8
The effects of the chemical modification on immobilized lipase features are affected by the enzyme crowding in the support	8
Effect of adenosine and cordycepin on recombinant antibody production yields in two different Chinease hamster ovary cell lines	8
Pre‐folding purification procedures for inclusion body‐derived non‐tagged cationic recombinant proteins with multiple disulfide bonds for efficient refolding	8
Lignin degradation by microorganisms: A review	7
Engineering tumor constructs to study matrix‐dependent angiogenic signaling of breast cancer cells	7
Effect of nucleophilic additives on phosphoric acid pretreatment of lignocelluloses	7
CD133 ligand‐enhanced etoposide‐liposome complex for targeted killing of lung cancer cells	7
Real time on‐line amino acid analysis to explore amino acid trends and link to glycosylation outcomes of a model monoclonal antibody	7
Analysis of tradeoffs between purification factor and yield for high‐performance countercurrent membrane purification for protein separations	7
Process analytics 4.0: A paradigm shift in rapid analytics for biologics development	7
Process optimization mitigated the retention loss of an Fc‐fusion protein during ultrafiltration/diafiltration	7
pH and conductivity transients during elution of IgG from protein A columns	6
Current status of platelet manufacturing in 3D or bioreactors	6
Dentin regeneration based on tooth tissue engineering: A review	6
Issue Information	6
Xanthan gum enhances peripheral blood CIK cells cytotoxicity in serum‐free medium	6
Issue Information	6
Removal of chromatin by salt‐tolerant endonucleases for production of recombinant measles virus	6
Assessment of shock pretreatment and alkali pretreatment on corn stover using enzymatic hydrolysis	6
Evaluating multiproduct chromatography Protein A resin reuse for monoclonal antibodies in biopharmaceutical manufacturing	6

Reshaping cell line development and CMC strategy for fast responses to pandemic outbreak	6
Mechanistic insights into hypoxia‐induced metabolic reprogramming in colorectal cancer through genome‐scale modeling	6
Methods for identifying precipitates and improving stability of chemically defined highly concentrated cell culture media	6
Evaluation of single‐use tangential flow filtration technology for purification of activated polysaccharides used in conjugate vaccine manufacturing	6
Modulation of high mannose levels in N‐linked glycosylation through cell culture process conditions to increase antibody‐dependent cell‐mediated cytotoxicity activity for an antibody biosimilar	5
Highly iterated palindrome 1 sequence improves Synechococcus sp. PCC 7002 transformation efficiencies in a homology‐ and methylation‐dependent manner	5
Characterization of Chinese hamster ovary cell culture feed media precipitate	5
Continuous CTC separation through a DEP‐based contraction–expansion inertial microfluidic channel	5
Genetically engineered neural stem cells (NSCs) therapy for neurological diseases; state‐of‐the‐art	5
A deep‐well plate enabled automated high‐throughput cell line development platform	5
CHOsynthetic promoters improve expression and product quality of biotherapeutic proteins	5
An automated high inoculation density fed‐batch bioreactor, enabled through N‐1 perfusion, accommodates clonal diversity and doubles titers	5
Process intensification of the baculovirus expression vector system using a perfusion process with a low multiplicity of infection at high cell concentrations	5
“Just add small molecules” cell‐free protein synthesis: CombiningDNAtemplate and cell extract preparation into a single fermentation	5
Advancing multiproduct resin reuse for development and clinical manufacturing of an antibody‐based therapeutic	5
Toward optimal clearance: A universal affinity‐based mass spectrometry approach for comprehensive ELISA reagent coverage evaluation and HCP hitchhiker analysis	5
Modeling the effect of oxidative stress on Bordetella pertussis fermentations	5
A modular and multi‐functional purification strategy that enables a common framework for manufacturing scale integrated and continuous biomanufacturing	5
Issue Information	5
Intramodule pressure profiles and protein accumulation during tangential flow filtration	5
Engineering a high‐throughput clone for industrial‐scale production of long‐acting GLP‐1 analogue with retained bio‐efficacy	5
Removal of phenolic inhibitors from lignocellulose hydrolysates using laccases for the production of fuels and chemicals	5
Issue Information	5
Probing morphological, genetic and metabolomic changes of in vitro embryo development in a microfluidic device	5
Proteomic analysis of host cell protein fouling during bioreactor harvesting	5
Examining organic acid production potential and growth‐coupled strategies in Issatchenkia orientalis using constraint‐based modeling	5
Fed‐batch performance profiles for mAb production using different intensified N − 1 seed strategies are CHO cell‐line dependent	5
Biotransformation of maize bran‐derived ferulic acid to vanillin using an adapted strain of Amycolatopsis sp. ATCC 39116	5
Bax and Bak knockout apoptosis‐resistant Chinese hamster ovary cell lines significantly improve culture viability and titer in intensified fed‐batch culture process	4
Efficient fermentative production of lactodifucotetraose by controlling sequential glycosyltransferase reactions in Escherichia coli	4
Exploring the potential of machine learning for more efficient development and production of biopharmaceuticals	4
Multi‐omics kinetic analysis of recombinant adeno‐associated virus production by plasmid transfection of HEK293 cells	4
Online monitored characterization of Phocaeicola vulgatus for organic acid production using anaerobic microtiter plate cultivations	4
The production of succinate with more CO2 fixation reactions facilitated by RuBisCO‐based engineered Escherichia coli	4
Transitioning from static to suspension culture system for large‐scale production of xeno‐free extracellular vesicles derived from mesenchymal stromal cells	4
Yeast cells for encapsulation of bioactive compounds in food products: A review	4
Synthesis of a novel nanocomposite containing chitosan as a three‐dimensional printed wound dressing technique: Emphasis on gene expression	4
Rapid intensification of an established CHO cell fed‐batch process	4
Tangential flow filtration‐facilitated purification of human red blood cell membrane fragments and its preferential use in removing unencapsulated material from resealed red blood cell ghosts compared	4
Effect of culture hydrodynamics on Arthrospira platensis production using a single‐use photobioreactor system through a CFD supported approach	4
Size‐based analysis of virus removal filter fouling using fractionated protein aggregates	4
Sources and methods of manufacturing xanthan by fermentation of various carbon sources	4
Assessing the probability of clonality achieved by single‐cell cloning of CHO cells through cell deposition combined with imaging using distinguishable cells	4
Transcriptomic features reveal molecular signatures associated with recombinant adeno‐associated virus production in HEK293 cells	4
Smart process development: Application of machine‐learning and integrated process modeling for inclusion body purification processes	4
Evaluating end‐to‐end continuous antibody manufacture with column‐free capture alternatives from economic, environmental, and robustness perspectives	4
A strategy for successful dual‐species protein expression of genes with non‐optimal codon usage destined for bacterial and yeast cell factories	4
Identification of compendial nonionic detergents for the replacement of Triton X‐100 in bioprocessing	4
Advanced strategies in glycosylation prediction and control during biopharmaceutical development: Avenues toward industry 4.0	4
Understanding the effect of temperature downshift onCHOcell growth, antibody titer and product quality by intracellular metabolite profiling and in vivo monitoring of redox state	3
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Measurement and control of foam generation in a mammalian cell culture	3
Impact of interstitial flow on cartilage matrix synthesis and NF‐kB transcription factor mRNA expression in a novel perfusion bioreactor	3
Effect of pH, NaCl concentration, and mAb concentration of feed solution on the filterability of Planova™ 20N and Planova™ BioEX	3
Modification of microstructure and selected physicochemical properties of bacterial cellulose produced by bacterial isolate using hydrocolloid‐fortified Hestrin–Schramm medium	3
Preventing pyruvate kinase muscle expression in Chinese hamster ovary cells curbs lactogenic behavior by altering glycolysis, gating pyruvate generation, and increasing pyruvate flux into t	3
Issue Information	3
PVA based nanofiber containing cellulose modified with graphitic carbon nitride/nettles/trachyspermum accelerates wound healing	3
Wound dressing based on PVA nanofiber containing silk fibroin modified with GO/ZnO nanoparticles for superficial wound healing: In vitro and in vivo evaluations	3
Biophysical properties of tense quaternary state polymerized human hemoglobins bracketed between 500 kDa and 0.2 μm in size	3
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High‐throughput in silico workflow for optimization and characterization of multimodal chromatographic processes	3
Adaptive modeling optimized by the data fusion strategy: Real‐time dying cell percentage prediction using capacitance spectroscopy	3
Self‐assembling protein nanocages for modular enzyme assembly by orthogonal bioconjugation	3
Advances in polymer‐based cell encapsulation and its applications in tissue repair	3
Utilizing targeted integration CHO pools to potentially accelerate the GMP manufacturing of monoclonal and bispecific antibodies	3
Issue Information	3
Polyhydroxyalkanoates and exopolysaccharides: An alternative for valuation of the co‐production of microbial biopolymers	3
Impact of iron raw materials and their impurities onCHOmetabolism and recombinant protein product quality	3
Intensified perfusion culture (IPC) reduced recombinant protein fragmentation	3
Tangential flow filtration facilitated fractionation of polymerized human serum albumin: Insights into the effects of molecular size on biophysical properties	3
Modulation of nutrient precursors for controlling metabolic inhibitors by genome‐scale flux balance analysis	3
Issue Information	3
Exploring the biocatalysis of psilocybin and other tryptamines: Enzymatic pathways, synthetic strategies, and industrial implications	2
Raman based chemometric model development for glycation and glycosylation real time monitoring in a manufacturing scale CHO cell bioreactor process	2
Pharmaceutical proteins at the interfaces and the role of albumin	2
Rheology of enzyme liquefied corn stover slurries: The effect of solids concentration on yielding and flow behavior	2
Chromatographic capture of cells to achieve single stage clarification in recombinant protein purification	2
Analytical characterization of host‐cell‐protein‐rich aggregates in monoclonal antibody solutions	2
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Application of mesenchymal stem cells in regenerative medicine: A new approach in modern medical science	2
Analytical characterization of broadly neutralizing antibody CAP256LS heavy chain clipping during manufacturing development	2
Current standards and pitfalls associated with the transfection of primary fibroblast cells	2
Preparation and characterization of poly (lactic‐co‐glycolic acid) nanofibers containing simvastatin coated with hyaluronic acid for using in periodontal tissue engineering	2
A new method to immobilize urease in silk fibroin membrane by unidirectional nanopore dehydration	2
A novel modified culture medium for enhancing redifferentiation of chondrocytes for cartilage tissue engineering applications	2
Integrated evaluation the antioxidant activity of epicatechin from cell dynamics	2
Integration of Planova filters in manufacturing processes of biologicals improve the virus safety effectively: A review of publicly available data	2
Issue Information	2
Antibody charge variant modulation by in vitro enzymatic treatment in different Chinese hamster ovary cell cultures	2
Improving outcomes in intensified processing via optimization of the cell line development workflow	2
Comprehensive studies on building a scalable downstream process for mRNAs to enable mRNA therapeutics	2
Depth filter material process interaction in the harvest of mammalian cells	2
Copper impurity of iron raw material contributes to improved cell culture performance	2
Antennapedia‐derived positively‐charged peptide faces multiple problems upon their usage as targeting ligand for liposomal doxorubicin	2
A novel method for continuous chromatographic separation of monoclonal antibody charge variants by combining displacement mode chromatography and step elution	2
Monitoring microalgal growth of Chlorella minutissima with a new all solid‐state contact nitrate selective sensor	2
Correlation of microbial community structure and volatile flavor compounds during corn yellow wine fermentation	2
Evaluation of PDL1 positive cancer cell‐specific binding activity of recombinant anti‐PDL1 scFv	2
Nanosensor based on HP‐MAP1 and carbon nanotubes for bacteria detection	2
Protein adsorption on core‐shell resins for flow‐through purifications: Effect of protein molecular size, shape, and salt concentration	2
Culturing Chinese hamster ovary cells on cyclo olefin polymer triggers epithelial‐mesenchymal transition and spheroid formation, which increases the foreign gene expression driven by the Moloney murin	2
Modification of C‐segment of CfaDnaE split intein for improving clean‐in‐place in chromatography process	2
Integrated micro‐scale protein a chromatography and Low pH viral inactivation unit operations on an automated platform	2
A synthetic platform for developing recombinant adeno‐associated virus type 8 producer cell lines	2
An innovative strategy to recycle permeate in biologics continuous manufacturing process to improve material efficiency and sustainability	2
A new strategy for the decellularization of whole organs by hydrostatic pressure	2
Development of virus‐like particles‐based vaccines against coronaviruses	2
A systematic approach to reduce intraocular pressure for the treatment of glaucoma	2
Accelerated CMC workflows to enable speed to clinic in the COVID‐19 era: A multi‐company view from the biopharmaceutical industry	2
Proceedings from the 3rd International Conference on Microbiome Engineering	2
Rapid metal speciation of cell culture media using reversed‐phase separations and inductively coupled plasma optical emission spectrometry	2
Raman spectroscopy and one‐dimensional convolutional neural network modeling as a real‐time monitoring tool for in vitro transaminase‐catalyzed synthesis of a pharmaceutically relevant amine precursor	2
Issue Information	2
Cover	2
Development and application of an analytical approach to assess an antibody's potential for disulfide reduction	2
Applications of flow cytometry sorting in the pharmaceutical industry: A review	1
Characterization of lipoprotein supplement and influence of its oxidized lipid content on cell culture performance and monoclonal antibody production by a SP2/0 hybridoma cell line	1
Demonstration of a robust high cell density transient CHO platform yielding mAb titers of up to 2 g/L without medium exchange	1
Optimization of an artificial neural network topology using response surface methodology for microbial fuel cell power prediction	1
Monte Carlo simulation‐guided design for size‐tuned tumor spheroid formation in 3D printed microwells	1
Stipulations of cell and gene therapy and the ties to biomanufacturing	1
Immobilized enzymes as potent antibiofilm agent	1
Determination of protein concentration in downstream biomanufacturing processes by in‐line index of refraction	1
Bioburden detection on surface and water samples in a rapid, ultra‐sensitive and high‐throughput manner	1
Development of chondrocyte‐laden alginate hydrogels with modulated microstructure and properties for cartilage regeneration	1
Improvement of the efficiency and quality in developing a new CHO host cell line	1
Harnessing cell aggregates for enhanced adeno‐associated virus manufacturing: Cultivation strategies and scale‐up considerations	1
Hydrolysis of lignocellulose by anaerobic fungi produces free sugars and organic acids for two‐stage fine chemical production with Kluyveromyces marxianus	1
Reusing wastewater from Coffea arabica processing to produce single‐cell protein using Candida sorboxylosa: Optimizing of culture conditions	1
Retrospective assessment of clonal origin of cell lines	1
Ionic liquid‐based in situ product removal design exemplified for an acetone–butanol–ethanol fermentation	1
Antibody glycation during a Chinese hamster ovary fed‐batch process is following a constrained second order reaction	1
Unraveling the potential and constraints associated with corn steep liquor as a nutrient source for industrial fermentations	1
Petroleum coke supplementation for enhanced biogas production and phosphate removal under mesophilic conditions	1
Use of scanning electron microscopy and energy dispersive X‐ray spectroscopy to identify key fouling species during alternating tangential filtration	1
Larvicidal potential of cell wall degrading enzymes from Trichoderma asperellum against Aedes aegypti (Diptera: Culicidae)	1
Single‐stage chromatographic clarification of Chinese Hamster Ovary cell harvest reduces cost of protein production	1
A method for facile production of variable lymphocyte receptors using SHuffle Escherichia coli	1
Enhancing the performance of an in vitro RNA biosensor through iterative design of experiments	1
Developments of biobased plasticizers for compostable polymers in the green packaging applications: A review	1
Flocculation‐based clarification for production of protein therapeutics in Pichia pastoris: Recombinant human serum albumin as a case study	1
Optimization and feedback control system of dilution rate for 1,3‐propanediol in two‐stage fermentation: A theoretical study	1
GMP implementation of a hybrid continuous manufacturing process for a recombinant non‐mAb protein—A case study	1
A design space for the filtration of challenging monoclonal antibodies using Planova™ S20N, a new regenerated cellulose virus removal filter	1
Hypoxia preconditioned mesenchymal stem cell‐derived exosomes induce ex vivo expansion of umbilical cord blood hematopoietic stem cells CD133+ by stimulation of Notch signaling pathway	1
An updated overview of the application of CAR‐T cell therapy in neurological diseases	1
Design and optimization of membrane chromatography for monoclonal antibody charge variant separation	1
Impact of different trace elements on metabolic routes during heterotrophic growth of C. ljungdahlii investigated through online measurement of the carbon dioxide transfer rate	1
Efficiency of ultrafiltration/diafiltration in removing organic and elemental process equipment related leachables from biological therapeutics	1
Corrections to: Soluble production and function of vascular endothelial growth factor/basic fibroblast growth factor complex peptide	1
Issue Information	1
Identification and classification of host cell proteins during biopharmaceutical process development	1
Use of Monte Carlo simulations for improved facility fit planning in downstream biomanufacturing and technology transfer	1
In‐line prediction of viability and viable cell density through machine learning‐based soft sensor modeling and an integrated systems approach: An industrially relevant PAT case study	1
A novel NCI‐H69V small cell lung cancer functional mini‐tumor model for future treatment screening applications	1
One‐step production of fully biotinylated and glycosylated human Fc gamma receptors	1
Insights into the morphology‐productivity relationship of filamentous fungi through small‐scale cultivation and automated microscopy of Thermothelomyces thermophilus	1
5‐Aza combined with VPA reprograms human T lineage acute leukemia Jurkat cells into B‐cell‐like cells by epigenetic activation of PAX5	1
Development of a miniature bioreactor model to study the impact of pH and DOT fluctuations on CHO cell culture performance as a tool to understanding heterogeneity eff	1
Mechano‐regulation of Wnt/β‐Catenin signaling to control paraxial versus lateral mesoderm lineage bifurcation	1
Iterative hybrid model based optimization of rAAV production	1
Gene copy number, gene configuration and LC/HC mRNA ratio impact on antibody productivity and product quality in targeted integration CHO cell lines	1
Understanding adsorption behavior of Polysorbate‐20 to sterile filters in therapeutic proteins final filtration process	1
Protein G: β‐galactosidase fusion protein for multi‐modal bioanalytical applications	1
In silico optimization of a challenging bispecific antibody chromatography step	1
Chitosan based hybrid superabsorbent for controlled drug delivery application	1
Efficient enzymatic synthesis ofL‐ascorbyl palmitate usingCandida antarcticalipase B‐embedded metal‐organic framework	1
Design of a bactericidal hydrogel scaffold containing genipin crosslinked HF‐18 peptide	1
Correction to “Investigation of the hepatic respiration and liver zonation on rat hepatocytes using an integrated oxygen biosensor in a microscale device”	1
Efficient immobilized phospholipase A1 on Mo‐basing nanomaterials for enzymatic degumming	1