Biotechnology Progress

Article	Citations
From protein structure to an optimized chromatographic capture step using multiscale modeling	120
Issue Information	71
Acetate accumulation and regulation by process parameters control in Chinese hamster ovary cell culture	51
Optimized pulse‐feeding fed‐batch fermentation for enhanced lignin to polyhydroxyalkanoate transformation	42
Proteomics‐based method to comprehensively model the removal of host cell protein impurities	41
Dynamic pH profiles drive higher cell‐specific and volumetric productivity	39
Exploring the design space for T riton X ‐100 substitutes in viral inactivation applications	27
Dynamic flux balance analysis of 1,3‐propanediol production by clostridium butyricum fermentation	23
Pre‐folding purification procedures for inclusion body‐derived non‐tagged cationic recombinant proteins with multiple disulfide bonds for efficient refolding	23
Depth filtration for clarification of intensified lentiviral vector suspension cell culture	23
High‐yield recombinant adeno‐associated viral vector production by multivariate optimization of bioprocess and transfection conditions	22
The effects of the chemical modification on immobilized lipase features are affected by the enzyme crowding in the support	22
Issue Information	20
The production of succinate with more CO 2 fixation reactions facilitate	19
Enrichment of full AAV capsids by preparative strong anion exchange chromatography	19
Evaluating multiproduct chromatography Protein A resin reuse for monoclonal antibodies in biopharmaceutical manufacturing	19
Genetically engineered neural stem cells (NSCs) therapy for neurological diseases; state‐of‐the‐art	18
Current status of platelet manufacturing in 3D or bioreactors	17
Removal of chromatin by salt‐tolerant endonucleases for production of recombinant measles virus	16
Online monitored characterization of Phocaeicola vulgatus for organic acid production using anaerobic microtiter plate cultivations	16
Modification of microstructure and selected physicochemical properties of bacterial cellulose produced by bacterial isolate using hydrocolloid‐fortified Hestrin–Schramm medium	16
Copper impurity of iron raw material contributes to improved cell culture performance	15
Unraveling the potential and constraints associated with corn steep liquor as a nutrient source for industrial fermentations	15
Development of virus‐like particles‐based vaccines against coronaviruses	15
Raman spectroscopy and one‐dimensional convolutional neural network modeling as a real‐time monitoring tool for in vitro transaminase‐catalyzed synthesis of a pharmaceutically relevant amine precursor	15

Correlation of microbial community structure and volatile flavor compounds during corn yellow wine fermentation	15
Hands‐free from inoculation to harvest: Microbial fermentation with multivariate model to automate induction of recombinant protein expression	15
Correction to “Investigation of the hepatic respiration and liver zonation on rat hepatocytes using an integrated oxygen biosensor in a microscale device”	14
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Immobilized enzymes as potent antibiofilm agent	14
Wound dressing based on PVA nanofiber containing silk fibroin modified with GO /	14
Antibody glycation during a Chinese hamster ovary fed‐batch process is following a constrained second order reaction	13
Development and application of an analytical approach to assess an antibody's potential for disulfide reduction	13
A novel modified culture medium for enhancing redifferentiation of chondrocytes for cartilage tissue engineering applications	13
Automation of an integrated micro‐scale platform for monoclonal antibody process development by incorporation of a depth filter mimic	13
Issue Information	13
Improved endotoxin removal using ecofriendly detergents for intensified plasmid capture	12
Impact of X‐ray irradiation as an equivalent alternative to gamma for sterilization of single‐use bioprocessing polymers	12
Hypoxia preconditioned mesenchymal stem cell‐derived exosomes induce ex vivo expansion of umbilical cord blood hematopoietic stem cells CD133 + by st	12
Petroleum coke supplementation for enhanced biogas production and phosphate removal under mesophilic conditions	12
Characterization of lipoprotein supplement and influence of its oxidized lipid content on cell culture performance and monoclonal antibody production by a SP2/0 hybridoma cell line	11
The development of an automated microscope image tracking and analysis system	11
Non‐sterile cultivation of Yarrowia lipolytica in fed‐batch mode for the production of lipids and biomass	11
Non‐thermal plasma decontamination of microbes: a state of the art	10
Improving trans‐cinnamic acid production in a model cyanobacterium	10
Proliferation and differentiation of primary bovine myoblasts using Chlorella vulgaris extract for sustainable production of cultured meat	10
Enhanced production of isobutyl and isoamyl acetate using Yarrowia lipolytica	10
Shear stress and microRNAs for better metastatic cancer management	10
Bioburden detection on surface and water samples in a rapid, ultra‐sensitive and high‐throughput manner	10
General strategies for IgG‐like bispecific antibody purification	9
When will we have a clone? An industry perspective on the typical CLD timeline	9
Combining regulated and constitutive protein expression significantly boosts protein expression by increasing productivity without affecting CHO cell	9
Bioengineered chimeric tRNA / pre‐miRNAs as prodrugs in cancer therapy	9
A simple electrochemical genosensor based on polythiophene acetic acid film for detection of Schistosoma mansoni	9
Primary clarification of mammalian cell culture fluid using enhanced sedimentation on inclined surfaces inside the single‐use disposable Sudhin BioSettler150	9
Surface decoration of low molecular weight polyethylenimine ( LMW PEI ) by phthalated dextrin for improved delivery of interleukin‐12 plasmid	9
Accelerating IND‐enabling toxicology studies using protein products from stable pools or pools of clones in Chinese hamster ovary cells	9
Enhancing cell separation in a hybrid spiral dielectrophoretic microchannel: Numerical insights and optimal operating conditions	9
Effect of light fluctuations on photosynthesis and metabolic flux in Synechocystis sp. PCC 6803	9
A systemic approach to identifying sequence frameworks that decrease mAb production in a transient Chinese hamster ovary cel	9
Biotechnological potential of yeast cell wall: An overview	8
Metabolic engineering of rapidly growing Synechococcus elongatus strains for phototrophic producti	8
Amino acid abundance and composition in cell culture medium affects trace metal tolerance and cholesterol synthesis	8
Use of single analytic tool to quantify both absolute N‐glycosylation and glycan distribution in monoclonal antibodies	8
Issue Information	8
Comparison of multi‐column chromatography configurations through model‐based optimization	7
Hybrid modeling for in silico optimization of a dynamic perfusion cell culture process	7
An adaptive modeling approach using spiking‐augmentation method to improve chemometric model performance in bioprocess monitoring	7
Modeling scalability of impurity precipitation in downstream biomanufacturing	7
Transmission of poly( dT 60 )	7
Optimization of amino acid composition in CHO cell perfusion medium using definitive screening design and	7
Online measurement of the viscosity in shake flasks enables monitoring of γ‐PGA production in depolymerase knockout mutants of	7
Predictive modeling of single pass tangential flow filtration for continuous biomanufacturing	7
Comparing in silico flowsheet optimization strategies in biopharmaceutical downstream processes	7
Issue Information	7

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A novel synthetic sRNA promoting protein overexpression in cell‐free systems	7
High‐sensitivity real‐time monitoring of pH and respiration activity unveils metabolic dynamics in shake flask cultures	7
A strategy for successful dual‐species protein expression of genes with non‐optimal codon usage destined for bacterial and yeast cell factories	6
Issue Information	6
Pharmaceutical proteins at the interfaces and the role of albumin	6
New technical concept for alternating tangential flow filtration in biotechnological cell separation processes	6
Continuous glucose feedback control using Raman spectroscopy and deep learning models for biopharmaceutical processes	6
Cation exchange as a single polishing step for conjugated peptides	6
Impact of bioreactor process parameters and yeast biomass on Raman spectra	6
Transcriptomic functional characterization of recombinant adeno‐associated virus producing cell line adapted to suspension‐growth	6
Polyhydroxyalkanoates and exopolysaccharides: An alternative for valuation of the co‐production of microbial biopolymers	6
Metabolic trends of Chinese hamster ovary cells in biopharmaceutical production under batch and fed‐batch conditions	6
Process optimization mitigated the retention loss of an Fc‐fusion protein during ultrafiltration/diafiltration	6
Exploring the potential of machine learning for more efficient development and production of biopharmaceuticals	6
Effects of LEDs, macronutrients and culture conditions on biomass and artemisinin production using Artemisia annuaL. suspension cultures	6
Optimization of carotenoid production by Umbelopsis ramanniana	6
Identification of compendial nonionic detergents for the replacement of Triton X‐100 in bioprocessing	6
Size‐based analysis of virus removal filter fouling using fractionated protein aggregates	6
A new method to immobilize urease in silk fibroin membrane by unidirectional nanopore dehydration	5
Modulation of nutrient precursors for controlling metabolic inhibitors by genome‐scale flux balance analysis	5
A systematic approach to reduce intraocular pressure for the treatment of glaucoma	5
Special issue on integrated continuous biomanufacturing	5
Synthetic modeling: A cell‐free approach for faster implementation of Raman spectroscopy in cell culture	5
Chitosan based hybrid superabsorbent for controlled drug delivery application	5
Triton CG‐110 as an alternative for cell lysis in manufacturing of adeno‐associated virus‐based gene therapy	5
Production of 8,11‐dihydroxy fatty acids from oleic and palmitoleic acids by Escherichia coli cells expressing variant 6,8‐linoleate diol synthases from	5
Modification of C‐segment of Cfa DnaE split intein for improving	5
A synthetic platform for developing recombinant adeno‐associated virus type 8 producer cell lines	5
Design of a bactericidal hydrogel scaffold containing genipin crosslinked HF ‐18 peptide	5
Integrated approach for purification of uricase and protease from Bacillus licheniformis by TPP and	5
Adapting virus filtration to continuous processing: Effects of product and process variability on filtration performance	5
Issue Information	5
Intensified perfusion culture (IPC) reduced recombinant protein fragmentation	5
Antibody charge variant modulation by in vitro enzymatic treatment in different Chinese hamster ovary cell cultures	5
Harnessing cell aggregates for enhanced adeno‐associated virus manufacturing: Cultivation strategies and scale‐up considerations	5
Issue Information	5
Isoelectric point ( pI )‐based phase separation ( pI‐BPS ) purification of elastin‐like polypeptid	5
A low‐cost, high‐throughput microfluidic nano‐culture platform for functional metagenomics	5
Efficient optimization of time‐varying inputs in a fed‐batch cell culture process using design of dynamic experiments	5
Effect of adenosine and cordycepin on recombinant antibody production yields in two different Chinease hamster ovary cell lines	4
Analysis of tradeoffs between purification factor and yield for high‐performance countercurrent membrane purification for protein separations	4
Proliferation of mammalian cells with Chlorococcum littorale algal compounds without serum support	4
Effects of green light supplementation with red and blue combinations of LED light spectrums on the growth and transcriptional response of Haematococcus pluvialis	4
Managing integrated continuous bioprocesses in real time: Deviations in product quality	4
Simplifying stable CHO cell line generation with high probability of monoclonality by using microfluidic dispensing as an alternative to fluorescence	4
Development of multiparametric bioprinting method for generation of 3D printed cell‐laden structures	4
Predictive mechanistic model for separation of monoclonal antibody, fab fragment, and aggregate species on multimodal chromatography	4
Real‐time online monitoring of insect cell proliferation and baculovirus infection using digital differential holographic microscopy and machine learning	4
Biotransformation of maize bran‐derived ferulic acid to vanillin using an adapted strain of Amycolatopsis sp. ATCC 39116	4
Aptamer‐functionalized quantum dots as theranostic nanotools against cancer and bacterial infections: A comprehensive overview of recent trends	4
Difficult‐to‐express antigen generation through a co‐expression and disassociation methodology	4
Just passing through: Deploying aquaporins in microbial cell factories	4
Impedimetric nanoimmunosensor platform for aflatoxin B1 detection in peanuts	4
Application of a fluorescent dye‐based microfluidic sensor for real‐time detection of mAb aggregates	4
Application of Ambr15 system for simulation of entire SARS‐CoV ‐2 vaccine production process involving macrocarriers	4
Issue Information	4
Relationship between intracellular protein denaturation and irreversible inactivation of Saccharomyces pastorianus by low‐pressure carbon dioxide microbu	4
Cryopreservation practices in clinical and preclinical iPSC‐based cell therapies: Current challenges and future directions	4
Effect of flux and shear rate on E. coli recovery in tangential flow filtration through a single hollow fiber	4
Role of membrane structure on the filtrate flux during monoclonal antibody filtration through virus retentive membranes	4
The combination of oleic acid, linoleic acid, palmitoleic acid, and α‐linolenic acid promoted the expansion of NK ‐92 cells in vitro	4
Application of fucosylation inhibitors for production of afucosylated antibody	4
Enhanced cell‐specific productivity through delayed supplementation of antioxidants in intensified processes	4
Environmental conditions during glycerol bioconversion affect 3‐hydroxypropionic acid bioproduction by Limosilactobacillus reuteri DSM	4
Application of inline variable pathlength technology for rapid determination of dynamic binding capacity in downstream process development of biopharmaceuticals	4
Model validation and selection in metabolic flux analysis and flux balance analysis	4
Optimizing monoclonal antibody biosimilar production via transfer and active learning for targeted quality profiles	4
An updated overview of the application of CAR‐T cell therapy in neurological diseases	3
Integration of Planova filters in manufacturing processes of biologicals improve the virus safety effectively: A review of publicly available data	3
Corrections to: Soluble production and function of vascular endothelial growth factor/basic fibroblast growth factor complex peptide	3
Integrated micro‐scale protein a chromatography and Low pH viral inactivation unit operations on an automated platform	3
Sources and methods of manufacturing xanthan by fermentation of various carbon sources	3
An automated high inoculation density fed‐batch bioreactor, enabled through N‐1 perfusion, accommodates clonal diversity and doubles titers	3
Evaluating end‐to‐end continuous antibody manufacture with column‐free capture alternatives from economic, environmental, and robustness perspectives	3
Highly iterated palindrome 1 sequence improves Synechococcus sp. PCC 7002 transformation efficiencies	3
Accelerated CMC workflows to enable speed to clinic in the COVID ‐19 era: A multi‐company view fro	3
High‐throughput in silico workflow for optimization and characterization of multimodal chromatographic processes	3
Enhancement of D‐mannitol production by fine‐tuned expression of mannitol‐1‐phosphate dehydrogenase in Synechocystis sp. PCC6803	3
Exploring the biocatalysis of psilocybin and other tryptamines: Enzymatic pathways, synthetic strategies, and industrial implications	3

Development of chondrocyte‐laden alginate hydrogels with modulated microstructure and properties for cartilage regeneration	3
Minimizing batch‐to‐batch variability of a live virus vaccine by process analytical technologies	3
Modeling the effect of oxidative stress on Bordetella pertussis fermentations	3
Removal of phenolic inhibitors from lignocellulose hydrolysates using laccases for the production of fuels and chemicals	3
Real time on‐line amino acid analysis to explore amino acid trends and link to glycosylation outcomes of a model monoclonal antibody	3
Optimization and feedback control system of dilution rate for 1,3‐propanediol in two‐stage fermentation: A theoretical study	3
Advances in polymer‐based cell encapsulation and its applications in tissue repair	3
Analytical characterization of host‐cell‐protein‐rich aggregates in monoclonal antibody solutions	3
Development of a miniature bioreactor model to study the impact of pH and DOT fluctuations on	3
Comprehensive studies on building a scalable downstream process for mRNAs to enable mRNA therapeut	3
Transcriptomic features reveal molecular signatures associated with recombinant adeno‐associated virus production in HEK293 cells	3
Tangential flow filtration‐facilitated purification of human red blood cell membrane fragments and its preferential use in removing unencapsulated material from resealed red blood cell ghosts compared	3
Multi‐omics kinetic analysis of recombinant adeno‐associated virus production by plasmid transfection of HEK293 cells	3
Identification and classification of host cell proteins during biopharmaceutical process development	3
Process intensification of the baculovirus expression vector system using a perfusion process with a low multiplicity of infection at high cell conce	3
Issue Information	3
Mechanistic modeling, simulation, and optimization of mixed‐mode chromatography for an antibody polishing step	2
Bio‐based composite granules with simultaneous biocontrol and phosphorus fertilization roles: Outcomes from a lab‐scale in vitro assessment	2
Assessment of membrane‐based downstream purification processes as a replacement to traditional resin bead for monoclonal antibody purification	2
Host cell proteins in monoclonal antibody processing: Control, detection, and removal	2
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Investigation of a failed DNA assay leads to identification of an unexpected contaminant in a commonly used chromatography buffer	2
Design and optimization of membrane chromatography for monoclonal antibody charge variant separation	2
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Enhancing cryopreservation of human induced pluripotent stem cells: Bottom‐up versus conventional freezing geometry	2
Advanced strategies in glycosylation prediction and control during biopharmaceutical development: Avenues toward industry 4.0	2
Understanding the effect of temperature downshift on CHO cell growth, antibody titer and product quality by intracellular metabolite profiling and in vivo monitoring of redox state	2
Smart process development: Application of machine‐learning and integrated process modeling for inclusion body purification processes	2
Correction to “Soluble production and function of vascular endothelial growth factor/basic fibroblast growth factor complex peptide”	2
Recent trends in biotechnological production, engineering, and applications of lysophospholipases	2
Evaluation of lentiviral vector stability and development of ion exchange purification processes	2
Characterization of large transgene integrations in Chinese hamster ovary cells using a bioengineered mammalian transposase	2
Identification of infectious viruses for risk‐based virus testing of CHO unprocessed bulk using next‐generation sequencing	2
Digital application for drug product potency target evaluation in biopharmaceutical manufacturing	2
Secondary feed filtration and storage conditions influence trace element availability and process performance at 2000  L scale	2
A novel NCI‐H69V small cell lung cancer functional mini‐tumor model for future treatment screening applications	2
Issue Information	2
Glass microspargers as effective frit spargers in single use bioreactors	2
A digital shadow of CAR T cell expansion in a perfusion bioreactor: Informing optimal harvest times for autologous cell therapy	2
N‐1 semi‐continuous transient perfusion in shake flask for ultra‐high density seeding of CHO cell cultures in benchtop bioreactors	2
Examining organic acid production potential and growth‐coupled strategies in Issatchenkia orientalis using constraint‐based modeling	2
Efficient fermentative production of lactodifucotetraose by controlling sequential glycosyltransferase reactions in Escherichia coli	2
A modular and multi‐functional purification strategy that enables a common framework for manufacturing scale integrated and continuous biomanufacturing	2
Implementation of mDoE ‐methods to a microcarrier‐based expansion processes for mesenchymal stem cells	2
Development of targeted gene delivery system based on liposome and PAMAM dendrimer functionalized with hyaluronic acid and T	2
Issue Information	2
Issue Information	2
Strategies to improve CHO cell culture performance: Targeted deletion of amino acid catabolism and apoptosis genes paired with growth inhibitor suppl	2
Efficient immobilized phospholipase A1 on Mo‐basing nanomaterials for enzymatic degumming	2
A comparison of different intensified upstream processes highlighting the advantage of WuXi Biologics' Ultra‐high Productivity platform ( WuXiUP	2
Changes to culture pH and dissolved oxygen can enhance chimeric antigen receptor T‐cell generation and differentiation	2
Mesenchymal stem cells derived‐exosomes enhanced amniotic membrane extract promotes corneal keratocyte proliferation	2
Optimization of an artificial neural network topology using response surface methodology for microbial fuel cell power prediction	2
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Use of ultrafiltration/diafiltration for the processing of antisense oligonucleotides	2
Xanthan gum enhances peripheral blood CIK cells cytotoxicity in serum‐free medium	2
Dentin regeneration based on tooth tissue engineering: A review	2
Methods comparison of two‐dimensional gel electrophoresis for host cell protein characterization	2