Biotechnology Progress

Article	Citations
Issue Information	101
Depth filtration for clarification of intensified lentiviral vector suspension cell culture	69
From protein structure to an optimized chromatographic capture step using multiscale modeling	65
Retrospective assessment of clonality of a legacy cell line by analytical subcloning of the master cell bank	50
Dynamic flux balance analysis of 1,3‐propanediol production by clostridium butyricum fermentation	39
Pre‐folding purification procedures for inclusion body‐derived non‐tagged cationic recombinant proteins with multiple disulfide bonds for efficient refolding	37
Optimized pulse‐feeding fed‐batch fermentation for enhanced lignin to polyhydroxyalkanoate transformation	31
Acetate accumulation and regulation by process parameters control in Chinese hamster ovary cell culture	27
The effects of the chemical modification on immobilized lipase features are affected by the enzyme crowding in the support	26
Proteomics‐based method to comprehensively model the removal of host cell protein impurities	25
Establishment and characterization of lung co‐culture spheroids for paclitaxel loaded Eudragit® RL 100 nanoparticle evaluation	22
Exometabolome profiling reveals activation of the carnitine buffering pathway in fed‐batch cultures ofCHOcells co‐fed with glucose and lactic acid	21
The production of succinate with more CO2 fixation reactions facilitated by RuBisCO‐based engineered Escherichia coli	20
Evaluating multiproduct chromatography Protein A resin reuse for monoclonal antibodies in biopharmaceutical manufacturing	20
High‐yield recombinant adeno‐associated viral vector production by multivariate optimization of bioprocess and transfection conditions	20
Current status of platelet manufacturing in 3D or bioreactors	19
Online monitored characterization of Phocaeicola vulgatus for organic acid production using anaerobic microtiter plate cultivations	17
Genetically engineered neural stem cells (NSCs) therapy for neurological diseases; state‐of‐the‐art	16
Removal of chromatin by salt‐tolerant endonucleases for production of recombinant measles virus	16
Issue Information	16
	15
Unraveling the potential and constraints associated with corn steep liquor as a nutrient source for industrial fermentations	15
Development and application of an analytical approach to assess an antibody's potential for disulfide reduction	15
A novel modified culture medium for enhancing redifferentiation of chondrocytes for cartilage tissue engineering applications	15
Copper impurity of iron raw material contributes to improved cell culture performance	15

Modification of microstructure and selected physicochemical properties of bacterial cellulose produced by bacterial isolate using hydrocolloid‐fortified Hestrin–Schramm medium	15
Wound dressing based on PVA nanofiber containing silk fibroin modified with GO/ZnO nanoparticles for superficial wound healing: In vitro and in vivo evaluations	14
Issue Information	14
Immobilized enzymes as potent antibiofilm agent	14
Raman spectroscopy and one‐dimensional convolutional neural network modeling as a real‐time monitoring tool for in vitro transaminase‐catalyzed synthesis of a pharmaceutically relevant amine precursor	13
Impact of X‐ray irradiation as an equivalent alternative to gamma for sterilization of single‐use bioprocessing polymers	13
Development of virus‐like particles‐based vaccines against coronaviruses	13
Bioburden detection on surface and water samples in a rapid, ultra‐sensitive and high‐throughput manner	13
Correction to “Investigation of the hepatic respiration and liver zonation on rat hepatocytes using an integrated oxygen biosensor in a microscale device”	13
Correlation of microbial community structure and volatile flavor compounds during corn yellow wine fermentation	13
Non‐sterile cultivation of Yarrowia lipolytica in fed‐batch mode for the production of lipids and biomass	13
Antibody glycation during a Chinese hamster ovary fed‐batch process is following a constrained second order reaction	12
Characterization of lipoprotein supplement and influence of its oxidized lipid content on cell culture performance and monoclonal antibody production by a SP2/0 hybridoma cell line	12
Petroleum coke supplementation for enhanced biogas production and phosphate removal under mesophilic conditions	12
The development of an automated microscope image tracking and analysis system	12
Improved endotoxin removal using ecofriendly detergents for intensified plasmid capture	12
Enhanced production of isobutyl and isoamyl acetate using Yarrowia lipolytica	11
General strategies for IgG‐like bispecific antibody purification	11
Hypoxia preconditioned mesenchymal stem cell‐derived exosomes induce ex vivo expansion of umbilical cord blood hematopoietic stem cells CD133+ by stimulation of Notch signaling pathway	11
When will we have a clone? An industry perspective on the typical CLD timeline	11
Shear stress and microRNAs for better metastatic cancer management	11
Primary clarification of mammalian cell culture fluid using enhanced sedimentation on inclined surfaces inside the single‐use disposable Sudhin BioSettler150	11
A systemic approach to identifying sequence frameworks that decrease mAb production in a transient Chinese hamster ovary cell expression system	10
Enhancing cell separation in a hybrid spiral dielectrophoretic microchannel: Numerical insights and optimal operating conditions	10
Combining regulated and constitutive protein expression significantly boosts protein expression by increasing productivity without affecting CHO cell growth	10
Surface decoration of low molecular weight polyethylenimine (LMW PEI) by phthalated dextrin for improved delivery of interleukin‐12 plasmid	10
Hybrid fusion protein as a dual protease inhibitor for the healing of chronic wounds	10
A simple electrochemical genosensor based on polythiophene acetic acid film for detection of Schistosoma mansoni	9
Bioengineered chimeric tRNA/pre‐miRNAs as prodrugs in cancer therapy	9
Non‐thermal plasma decontamination of microbes: a state of the art	9
Improving trans‐cinnamic acid production in a model cyanobacterium	9
Comparing in silico flowsheet optimization strategies in biopharmaceutical downstream processes	9
Accelerating IND‐enabling toxicology studies using protein products from stable pools or pools of clones in Chinese hamster ovary cells	9
Proliferation and differentiation of primary bovine myoblasts using Chlorella vulgaris extract for sustainable production of cultured meat	9
Effect of light fluctuations on photosynthesis and metabolic flux in Synechocystis sp. PCC 6803	9
Transmission of poly(dT60) single‐stranded DNA through polycarbonate track‐etched ultrafiltration membranes	8
High‐sensitivity real‐time monitoring of pH and respiration activity unveils metabolic dynamics in shake flask cultures	8
Metabolic engineering of rapidly growing Synechococcus elongatus strains for phototrophic production of alkanes	8
Issue Information	8
Issue Information	8
Use of single analytic tool to quantify both absolute N‐glycosylation and glycan distribution in monoclonal antibodies	8
Modeling scalability of impurity precipitation in downstream biomanufacturing	8
Comparison of multi‐column chromatography configurations through model‐based optimization	8
Amino acid abundance and composition in cell culture medium affects trace metal tolerance and cholesterol synthesis	8
	7
Online measurement of the viscosity in shake flasks enables monitoring of γ‐PGA production in depolymerase knockout mutants of Bacillus subtilis with the phosphate‐starvation inducib	7
Optimization of carotenoid production by Umbelopsis ramanniana	7
A novel synthetic sRNA promoting protein overexpression in cell‐free systems	7
Predictive modeling of single pass tangential flow filtration for continuous biomanufacturing	7
Cation exchange as a single polishing step for conjugated peptides	7

Metabolic trends of Chinese hamster ovary cells in biopharmaceutical production under batch and fed‐batch conditions	7
Biotechnological potential of yeast cell wall: An overview	7
An adaptive modeling approach using spiking‐augmentation method to improve chemometric model performance in bioprocess monitoring	7
Hybrid modeling for in silico optimization of a dynamic perfusion cell culture process	7
New technical concept for alternating tangential flow filtration in biotechnological cell separation processes	7
Impact of bioreactor process parameters and yeast biomass on Raman spectra	6
Transcriptomic functional characterization of recombinant adeno‐associated virus producing cell line adapted to suspension‐growth	6
Continuous glucose feedback control using Raman spectroscopy and deep learning models for biopharmaceutical processes	6
Issue Information	6
Harnessing cell aggregates for enhanced adeno‐associated virus manufacturing: Cultivation strategies and scale‐up considerations	6
Modification of C‐segment of CfaDnaE split intein for improving clean‐in‐place in chromatography process	6
PVA based nanofiber containing cellulose modified with graphitic carbon nitride/nettles/trachyspermum accelerates wound healing	6
Issue Information	6
Size‐based analysis of virus removal filter fouling using fractionated protein aggregates	6
Exploring the potential of machine learning for more efficient development and production of biopharmaceuticals	6
A new method to immobilize urease in silk fibroin membrane by unidirectional nanopore dehydration	6
Antibody charge variant modulation by in vitro enzymatic treatment in different Chinese hamster ovary cell cultures	6
Process optimization mitigated the retention loss of an Fc‐fusion protein during ultrafiltration/diafiltration	6
A strategy for successful dual‐species protein expression of genes with non‐optimal codon usage destined for bacterial and yeast cell factories	6
Identification of compendial nonionic detergents for the replacement of Triton X‐100 in bioprocessing	6
A systematic approach to reduce intraocular pressure for the treatment of glaucoma	6
Modulation of nutrient precursors for controlling metabolic inhibitors by genome‐scale flux balance analysis	6
A synthetic platform for developing recombinant adeno‐associated virus type 8 producer cell lines	6
Polyhydroxyalkanoates and exopolysaccharides: An alternative for valuation of the co‐production of microbial biopolymers	6
Efficient optimization of time‐varying inputs in a fed‐batch cell culture process using design of dynamic experiments	5
Effects of LEDs, macronutrients and culture conditions on biomass and artemisinin production using Artemisia annuaL. suspension cultures	5
Special issue on integrated continuous biomanufacturing	5
Simplifying stable CHO cell line generation with high probability of monoclonality by using microfluidic dispensing as an alternative to fluorescence activated cell sorting	5
Isoelectric point (pI)‐based phase separation (pI‐BPS) purification of elastin‐like polypeptides (ELPs) containing charged, biologically active fusion proteins (E	5
A low‐cost, high‐throughput microfluidic nano‐culture platform for functional metagenomics	5
Intensified perfusion culture (IPC) reduced recombinant protein fragmentation	5
Design of a bactericidal hydrogel scaffold containing genipin crosslinked HF‐18 peptide	5
Chitosan based hybrid superabsorbent for controlled drug delivery application	5
Synthetic modeling: A cell‐free approach for faster implementation of Raman spectroscopy in cell culture	5
Enhanced cell‐specific productivity through delayed supplementation of antioxidants in intensified processes	5
Application of fucosylation inhibitors for production of afucosylated antibody	5
Issue Information	5
Pharmaceutical proteins at the interfaces and the role of albumin	5
A ribonucleoprotein‐based decaplex CRISPR/Cas9 knockout strategy for CHO host engineering	5
The effect of biosynthesized selenium nanoparticles on the expression of CYP51A and HSP90 antifungal resistance genes in Aspergillus fumigatus and Aspergi	5
Adapting virus filtration to continuous processing: Effects of product and process variability on filtration performance	5
Application of a fluorescent dye‐based microfluidic sensor for real‐time detection of mAb aggregates	5
Production of 8,11‐dihydroxy fatty acids from oleic and palmitoleic acids by Escherichia coli cells expressing variant 6,8‐linoleate diol synthases from Penicillium oxalicum	5
Effect of adenosine and cordycepin on recombinant antibody production yields in two different Chinease hamster ovary cell lines	4
Relationship between intracellular protein denaturation and irreversible inactivation of Saccharomyces pastorianus by low‐pressure carbon dioxide microbubbles	4
Cryopreservation practices in clinical and preclinical iPSC‐based cell therapies: Current challenges and future directions	4
Aptamer‐functionalized quantum dots as theranostic nanotools against cancer and bacterial infections: A comprehensive overview of recent trends	4
Effects of green light supplementation with red and blue combinations of LED light spectrums on the growth and transcriptional response of Haematococcus pluvialis	4
Environmental conditions during glycerol bioconversion affect 3‐hydroxypropionic acid bioproduction byLimosilactobacillus reuteriDSM17938	4
Difficult‐to‐express antigen generation through a co‐expression and disassociation methodology	4
Predictive mechanistic model for separation of monoclonal antibody, fab fragment, and aggregate species on multimodal chromatography	4
Managing integrated continuous bioprocesses in real time: Deviations in product quality	4
Model validation and selection in metabolic flux analysis and flux balance analysis	4
Role of membrane structure on the filtrate flux during monoclonal antibody filtration through virus retentive membranes	4
Application of Ambr15 system for simulation of entire SARS‐CoV‐2 vaccine production process involving macrocarriers	4
Triton CG‐110 as an alternative for cell lysis in manufacturing of adeno‐associated virus‐based gene therapy	4
Application of inline variable pathlength technology for rapid determination of dynamic binding capacity in downstream process development of biopharmaceuticals	4
Just passing through: Deploying aquaporins in microbial cell factories	4
The combination of oleic acid, linoleic acid, palmitoleic acid, and α‐linolenic acid promoted the expansion of NK‐92 cells in vitro	4
Issue Information	4
Analysis of tradeoffs between purification factor and yield for high‐performance countercurrent membrane purification for protein separations	4
Effect of flux and shear rate on E. coli recovery in tangential flow filtration through a single hollow fiber	4
Impedimetric nanoimmunosensor platform for aflatoxin B1 detection in peanuts	4
Proliferation of mammalian cells with Chlorococcum littorale algal compounds without serum support	4
Development of multiparametric bioprinting method for generation of 3D printed cell‐laden structures	4
Tangential flow filtration‐facilitated purification of human red blood cell membrane fragments and its preferential use in removing unencapsulated material from resealed red blood cell ghosts compared	3
Transcriptomic features reveal molecular signatures associated with recombinant adeno‐associated virus production in HEK293 cells	3
Integration of Planova filters in manufacturing processes of biologicals improve the virus safety effectively: A review of publicly available data	3
Accelerated CMC workflows to enable speed to clinic in the COVID‐19 era: A multi‐company view from the biopharmaceutical industry	3
An updated overview of the application of CAR‐T cell therapy in neurological diseases	3
Development of chondrocyte‐laden alginate hydrogels with modulated microstructure and properties for cartilage regeneration	3
Corrections to: Soluble production and function of vascular endothelial growth factor/basic fibroblast growth factor complex peptide	3
Rapid intensification of an established CHO cell fed‐batch process	3
Highly iterated palindrome 1 sequence improves Synechococcus sp. PCC 7002 transformation efficiencies in a homology‐ and methylation‐dependent manner	3
Real time on‐line amino acid analysis to explore amino acid trends and link to glycosylation outcomes of a model monoclonal antibody	3
Modeling the effect of oxidative stress on Bordetella pertussis fermentations	3
Sources and methods of manufacturing xanthan by fermentation of various carbon sources	3
Analytical characterization of host‐cell‐protein‐rich aggregates in monoclonal antibody solutions	3
Identification and classification of host cell proteins during biopharmaceutical process development	3
High‐throughput in silico workflow for optimization and characterization of multimodal chromatographic processes	3

Comprehensive studies on building a scalable downstream process for mRNAs to enable mRNA therapeutics	3
Advances in polymer‐based cell encapsulation and its applications in tissue repair	3
Removal of phenolic inhibitors from lignocellulose hydrolysates using laccases for the production of fuels and chemicals	3
Evaluating end‐to‐end continuous antibody manufacture with column‐free capture alternatives from economic, environmental, and robustness perspectives	3
Multi‐omics kinetic analysis of recombinant adeno‐associated virus production by plasmid transfection of HEK293 cells	3
Real‐time online monitoring of insect cell proliferation and baculovirus infection using digital differential holographic microscopy and machine learning	3
Development of a miniature bioreactor model to study the impact of pH and DOT fluctuations on CHO cell culture performance as a tool to understanding heterogeneity eff	3
Integrated micro‐scale protein a chromatography and Low pH viral inactivation unit operations on an automated platform	3
Minimizing batch‐to‐batch variability of a live virus vaccine by process analytical technologies	3
Enhancement of D‐mannitol production by fine‐tuned expression of mannitol‐1‐phosphate dehydrogenase in Synechocystis sp. PCC6803	3
Optimization and feedback control system of dilution rate for 1,3‐propanediol in two‐stage fermentation: A theoretical study	3
Exploring the biocatalysis of psilocybin and other tryptamines: Enzymatic pathways, synthetic strategies, and industrial implications	3
Biotransformation of maize bran‐derived ferulic acid to vanillin using an adapted strain of Amycolatopsis sp. ATCC 39116	3
Process intensification of the baculovirus expression vector system using a perfusion process with a low multiplicity of infection at high cell concentrations	3
An automated high inoculation density fed‐batch bioreactor, enabled through N‐1 perfusion, accommodates clonal diversity and doubles titers	3
Recent trends in biotechnological production, engineering, and applications of lysophospholipases	2
“Just add small molecules” cell‐free protein synthesis: CombiningDNAtemplate and cell extract preparation into a single fermentation	2
A comparison of different intensified upstream processes highlighting the advantage of WuXi Biologics' Ultra‐high Productivity platform (WuXiUPTM) in improved product quality and	2
Bio‐based composite granules with simultaneous biocontrol and phosphorus fertilization roles: Outcomes from a lab‐scale in vitro assessment	2
Issue Information	2
Host cell proteins in monoclonal antibody processing: Control, detection, and removal	2
Assessment of membrane‐based downstream purification processes as a replacement to traditional resin bead for monoclonal antibody purification	2
Use of ultrafiltration/diafiltration for the processing of antisense oligonucleotides	2
Application of a simple unstructured kinetic and cost of goods models to support T‐cell therapy manufacture	2
Strategies to improve CHO cell culture performance: Targeted deletion of amino acid catabolism and apoptosis genes paired with growth inhibitor supplementation	2
Glass microspargers as effective frit spargers in single use bioreactors	2
Design and optimization of membrane chromatography for monoclonal antibody charge variant separation	2
Evaluation of lentiviral vector stability and development of ion exchange purification processes	2
Examining organic acid production potential and growth‐coupled strategies in Issatchenkia orientalis using constraint‐based modeling	2
Enhancing cryopreservation of human induced pluripotent stem cells: Bottom‐up versus conventional freezing geometry	2
Mesenchymal stem cells derived‐exosomes enhanced amniotic membrane extract promotes corneal keratocyte proliferation	2
Characterization of large transgene integrations in Chinese hamster ovary cells using a bioengineered mammalian transposase	2
Correction to “Soluble production and function of vascular endothelial growth factor/basic fibroblast growth factor complex peptide”	2
Issue Information	2
Investigation of a failed DNA assay leads to identification of an unexpected contaminant in a commonly used chromatography buffer	2
Development of targeted gene delivery system based on liposome and PAMAM dendrimer functionalized with hyaluronic acid and TAT peptide: In vitro and in vivo studies	2
	2
Changes to culture pH and dissolved oxygen can enhance chimeric antigen receptor T‐cell generation and differentiation	2
Metabolic engineering of Yarrowia lipolytica for the production of isoprene	2
Optimization of an artificial neural network topology using response surface methodology for microbial fuel cell power prediction	2
A digital shadow of CAR T cell expansion in a perfusion bioreactor: Informing optimal harvest times for autologous cell therapy	2
The pandemic and resilience for the future: AccBio 2021	2
Methods comparison of two‐dimensional gel electrophoresis for host cell protein characterization	2
Dentin regeneration based on tooth tissue engineering: A review	2
Mechanistic modeling, simulation, and optimization of mixed‐mode chromatography for an antibody polishing step	2
Digital application for drug product potency target evaluation in biopharmaceutical manufacturing	2
Implementation of mDoE‐methods to a microcarrier‐based expansion processes for mesenchymal stem cells	2
Identification of infectious viruses for risk‐based virus testing of CHO unprocessed bulk using next‐generation sequencing	2
Issue Information	2
Issue Information	2
Efficient immobilized phospholipase A1 on Mo‐basing nanomaterials for enzymatic degumming	2
A novel NCI‐H69V small cell lung cancer functional mini‐tumor model for future treatment screening applications	2