Biotechnology Progress

Article	Citations
Optimized pulse‐feeding fed‐batch fermentation for enhanced lignin to polyhydroxyalkanoate transformation	105
Depth filtration for clarification of intensified lentiviral vector suspension cell culture	69
Establishment and characterization of lung co‐culture spheroids for paclitaxel loaded Eudragit® RL 100 nanoparticle evaluation	66
Retrospective assessment of clonality of a legacy cell line by analytical subcloning of the master cell bank	50
Acetate accumulation and regulation by process parameters control in Chinese hamster ovary cell culture	41
The effects of the chemical modification on immobilized lipase features are affected by the enzyme crowding in the support	39
Proteomics‐based method to comprehensively model the removal of host cell protein impurities	32
Issue Information	27
From protein structure to an optimized chromatographic capture step using multiscale modeling	26
High‐yield recombinant adeno‐associated viral vector production by multivariate optimization of bioprocess and transfection conditions	22
Pre‐folding purification procedures for inclusion body‐derived non‐tagged cationic recombinant proteins with multiple disulfide bonds for efficient refolding	22
Dynamic flux balance analysis of 1,3‐propanediol production by clostridium butyricum fermentation	21
Genetically engineered neural stem cells (NSCs) therapy for neurological diseases; state‐of‐the‐art	20
Online monitored characterization of Phocaeicola vulgatus for organic acid production using anaerobic microtiter plate cultivations	20
Current status of platelet manufacturing in 3D or bioreactors	19
Removal of chromatin by salt‐tolerant endonucleases for production of recombinant measles virus	19
Issue Information	18
The production of succinate with more CO2 fixation reactions facilitated by RuBisCO‐based engineered Escherichia coli	17
Evaluating multiproduct chromatography Protein A resin reuse for monoclonal antibodies in biopharmaceutical manufacturing	17
Development of virus‐like particles‐based vaccines against coronaviruses	16
A novel modified culture medium for enhancing redifferentiation of chondrocytes for cartilage tissue engineering applications	16
Development and application of an analytical approach to assess an antibody's potential for disulfide reduction	15
Issue Information	15
Correction to “Investigation of the hepatic respiration and liver zonation on rat hepatocytes using an integrated oxygen biosensor in a microscale device”	15
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Raman spectroscopy and one‐dimensional convolutional neural network modeling as a real‐time monitoring tool for in vitro transaminase‐catalyzed synthesis of a pharmaceutically relevant amine precursor	14
Unraveling the potential and constraints associated with corn steep liquor as a nutrient source for industrial fermentations	14
Hands‐free from inoculation to harvest: Microbial fermentation with multivariate model to automate induction of recombinant protein expression	13
Correlation of microbial community structure and volatile flavor compounds during corn yellow wine fermentation	13
Modification of microstructure and selected physicochemical properties of bacterial cellulose produced by bacterial isolate using hydrocolloid‐fortified Hestrin–Schramm medium	13
Wound dressing based on PVA nanofiber containing silk fibroin modified with GO/ZnO nanoparticles for superficial wound healing: In vitro and in vivo evaluations	13
Immobilized enzymes as potent antibiofilm agent	13
Copper impurity of iron raw material contributes to improved cell culture performance	13
Bioburden detection on surface and water samples in a rapid, ultra‐sensitive and high‐throughput manner	12
The development of an automated microscope image tracking and analysis system	12
Improved endotoxin removal using ecofriendly detergents for intensified plasmid capture	12
Non‐sterile cultivation of Yarrowia lipolytica in fed‐batch mode for the production of lipids and biomass	12
Characterization of lipoprotein supplement and influence of its oxidized lipid content on cell culture performance and monoclonal antibody production by a SP2/0 hybridoma cell line	11
Hybrid fusion protein as a dual protease inhibitor for the healing of chronic wounds	11
Impact of X‐ray irradiation as an equivalent alternative to gamma for sterilization of single‐use bioprocessing polymers	11
Hypoxia preconditioned mesenchymal stem cell‐derived exosomes induce ex vivo expansion of umbilical cord blood hematopoietic stem cells CD133+ by stimulation of Notch signaling pathway	11
Petroleum coke supplementation for enhanced biogas production and phosphate removal under mesophilic conditions	11
Antibody glycation during a Chinese hamster ovary fed‐batch process is following a constrained second order reaction	11
Enhanced production of isobutyl and isoamyl acetate using Yarrowia lipolytica	11
Effect of light fluctuations on photosynthesis and metabolic flux in Synechocystis sp. PCC 6803	10
Primary clarification of mammalian cell culture fluid using enhanced sedimentation on inclined surfaces inside the single‐use disposable Sudhin BioSettler150	10
Combining regulated and constitutive protein expression significantly boosts protein expression by increasing productivity without affecting CHO cell growth	10
Shear stress and microRNAs for better metastatic cancer management	10
General strategies for IgG‐like bispecific antibody purification	10
Improving trans‐cinnamic acid production in a model cyanobacterium	10
When will we have a clone? An industry perspective on the typical CLD timeline	10
Bioengineered chimeric tRNA/pre‐miRNAs as prodrugs in cancer therapy	9
A simple electrochemical genosensor based on polythiophene acetic acid film for detection of Schistosoma mansoni	9
Non‐thermal plasma decontamination of microbes: a state of the art	9
Enhancing cell separation in a hybrid spiral dielectrophoretic microchannel: Numerical insights and optimal operating conditions	9
Proliferation and differentiation of primary bovine myoblasts using Chlorella vulgaris extract for sustainable production of cultured meat	9
A systemic approach to identifying sequence frameworks that decrease mAb production in a transient Chinese hamster ovary cell expression system	9
Surface decoration of low molecular weight polyethylenimine (LMW PEI) by phthalated dextrin for improved delivery of interleukin‐12 plasmid	9
Accelerating IND‐enabling toxicology studies using protein products from stable pools or pools of clones in Chinese hamster ovary cells	9
Comparing in silico flowsheet optimization strategies in biopharmaceutical downstream processes	8
Use of single analytic tool to quantify both absolute N‐glycosylation and glycan distribution in monoclonal antibodies	8
Amino acid abundance and composition in cell culture medium affects trace metal tolerance and cholesterol synthesis	8
Issue Information	8
Modeling scalability of impurity precipitation in downstream biomanufacturing	8
Issue Information	8
Metabolic engineering of rapidly growing Synechococcus elongatus strains for phototrophic production of alkanes	8
Optimization of carotenoid production by Umbelopsis ramanniana	7
Comparison of multi‐column chromatography configurations through model‐based optimization	7
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An adaptive modeling approach using spiking‐augmentation method to improve chemometric model performance in bioprocess monitoring	7
New technical concept for alternating tangential flow filtration in biotechnological cell separation processes	7
Biotechnological potential of yeast cell wall: An overview	7
Predictive modeling of single pass tangential flow filtration for continuous biomanufacturing	7
Cation exchange as a single polishing step for conjugated peptides	7
Online measurement of the viscosity in shake flasks enables monitoring of γ‐PGA production in depolymerase knockout mutants of Bacillus subtilis with the phosphate‐starvation inducib	7

Transmission of poly(dT60) single‐stranded DNA through polycarbonate track‐etched ultrafiltration membranes	7
High‐sensitivity real‐time monitoring of pH and respiration activity unveils metabolic dynamics in shake flask cultures	7
Hybrid modeling for in silico optimization of a dynamic perfusion cell culture process	7
A novel synthetic sRNA promoting protein overexpression in cell‐free systems	7
Issue Information	6
Metabolic trends of Chinese hamster ovary cells in biopharmaceutical production under batch and fed‐batch conditions	6
Exploring the potential of machine learning for more efficient development and production of biopharmaceuticals	6
Harnessing cell aggregates for enhanced adeno‐associated virus manufacturing: Cultivation strategies and scale‐up considerations	6
A systematic approach to reduce intraocular pressure for the treatment of glaucoma	6
Pharmaceutical proteins at the interfaces and the role of albumin	6
A strategy for successful dual‐species protein expression of genes with non‐optimal codon usage destined for bacterial and yeast cell factories	6
Transcriptomic functional characterization of recombinant adeno‐associated virus producing cell line adapted to suspension‐growth	6
Process optimization mitigated the retention loss of an Fc‐fusion protein during ultrafiltration/diafiltration	6
Modulation of nutrient precursors for controlling metabolic inhibitors by genome‐scale flux balance analysis	6
Antibody charge variant modulation by in vitro enzymatic treatment in different Chinese hamster ovary cell cultures	6
Size‐based analysis of virus removal filter fouling using fractionated protein aggregates	6
Impact of bioreactor process parameters and yeast biomass on Raman spectra	6
Identification of compendial nonionic detergents for the replacement of Triton X‐100 in bioprocessing	6
Continuous glucose feedback control using Raman spectroscopy and deep learning models for biopharmaceutical processes	6
Issue Information	6
Polyhydroxyalkanoates and exopolysaccharides: An alternative for valuation of the co‐production of microbial biopolymers	6
Issue Information	6
Issue Information	5
A synthetic platform for developing recombinant adeno‐associated virus type 8 producer cell lines	5
Special issue on integrated continuous biomanufacturing	5
Modification of C‐segment of CfaDnaE split intein for improving clean‐in‐place in chromatography process	5
Impedimetric nanoimmunosensor platform for aflatoxin B1 detection in peanuts	5
Isoelectric point (pI)‐based phase separation (pI‐BPS) purification of elastin‐like polypeptides (ELPs) containing charged, biologically active fusion proteins (E	5
Triton CG‐110 as an alternative for cell lysis in manufacturing of adeno‐associated virus‐based gene therapy	5
A new method to immobilize urease in silk fibroin membrane by unidirectional nanopore dehydration	5
Design of a bactericidal hydrogel scaffold containing genipin crosslinked HF‐18 peptide	5
Efficient optimization of time‐varying inputs in a fed‐batch cell culture process using design of dynamic experiments	5
The effect of biosynthesized selenium nanoparticles on the expression of CYP51A and HSP90 antifungal resistance genes in Aspergillus fumigatus and Aspergi	5
Simplifying stable CHO cell line generation with high probability of monoclonality by using microfluidic dispensing as an alternative to fluorescence activated cell sorting	5
Enhanced cell‐specific productivity through delayed supplementation of antioxidants in intensified processes	5
Synthetic modeling: A cell‐free approach for faster implementation of Raman spectroscopy in cell culture	5
Effects of LEDs, macronutrients and culture conditions on biomass and artemisinin production using Artemisia annuaL. suspension cultures	5
Intensified perfusion culture (IPC) reduced recombinant protein fragmentation	5
A ribonucleoprotein‐based decaplex CRISPR/Cas9 knockout strategy for CHO host engineering	5
Production of 8,11‐dihydroxy fatty acids from oleic and palmitoleic acids by Escherichia coli cells expressing variant 6,8‐linoleate diol synthases from Penicillium oxalicum	5
Chitosan based hybrid superabsorbent for controlled drug delivery application	5
Adapting virus filtration to continuous processing: Effects of product and process variability on filtration performance	5
Application of fucosylation inhibitors for production of afucosylated antibody	5
Integrated approach for purification of uricase and protease from Bacillus licheniformis by TPP and IEC	5
Effect of adenosine and cordycepin on recombinant antibody production yields in two different Chinease hamster ovary cell lines	4
Real‐time online monitoring of insect cell proliferation and baculovirus infection using digital differential holographic microscopy and machine learning	4
Predictive mechanistic model for separation of monoclonal antibody, fab fragment, and aggregate species on multimodal chromatography	4
A low‐cost, high‐throughput microfluidic nano‐culture platform for functional metagenomics	4
Application of inline variable pathlength technology for rapid determination of dynamic binding capacity in downstream process development of biopharmaceuticals	4
Just passing through: Deploying aquaporins in microbial cell factories	4
Issue Information	4
Relationship between intracellular protein denaturation and irreversible inactivation of Saccharomyces pastorianus by low‐pressure carbon dioxide microbubbles	4
Managing integrated continuous bioprocesses in real time: Deviations in product quality	4
Role of membrane structure on the filtrate flux during monoclonal antibody filtration through virus retentive membranes	4
The combination of oleic acid, linoleic acid, palmitoleic acid, and α‐linolenic acid promoted the expansion of NK‐92 cells in vitro	4
Aptamer‐functionalized quantum dots as theranostic nanotools against cancer and bacterial infections: A comprehensive overview of recent trends	4
Proliferation of mammalian cells with Chlorococcum littorale algal compounds without serum support	4
Effects of green light supplementation with red and blue combinations of LED light spectrums on the growth and transcriptional response of Haematococcus pluvialis	4
Effect of flux and shear rate on E. coli recovery in tangential flow filtration through a single hollow fiber	4
Cryopreservation practices in clinical and preclinical iPSC‐based cell therapies: Current challenges and future directions	4
Development of multiparametric bioprinting method for generation of 3D printed cell‐laden structures	4
Application of a fluorescent dye‐based microfluidic sensor for real‐time detection of mAb aggregates	4
Environmental conditions during glycerol bioconversion affect 3‐hydroxypropionic acid bioproduction byLimosilactobacillus reuteriDSM17938	4
Difficult‐to‐express antigen generation through a co‐expression and disassociation methodology	4
Application of Ambr15 system for simulation of entire SARS‐CoV‐2 vaccine production process involving macrocarriers	4
Analysis of tradeoffs between purification factor and yield for high‐performance countercurrent membrane purification for protein separations	3
Comprehensive studies on building a scalable downstream process for mRNAs to enable mRNA therapeutics	3
Development of a miniature bioreactor model to study the impact of pH and DOT fluctuations on CHO cell culture performance as a tool to understanding heterogeneity eff	3
Integrated micro‐scale protein a chromatography and Low pH viral inactivation unit operations on an automated platform	3
Development of chondrocyte‐laden alginate hydrogels with modulated microstructure and properties for cartilage regeneration	3
An updated overview of the application of CAR‐T cell therapy in neurological diseases	3
Identification and classification of host cell proteins during biopharmaceutical process development	3
Sources and methods of manufacturing xanthan by fermentation of various carbon sources	3
Real time on‐line amino acid analysis to explore amino acid trends and link to glycosylation outcomes of a model monoclonal antibody	3
Evaluating end‐to‐end continuous antibody manufacture with column‐free capture alternatives from economic, environmental, and robustness perspectives	3
An automated high inoculation density fed‐batch bioreactor, enabled through N‐1 perfusion, accommodates clonal diversity and doubles titers	3
Transcriptomic features reveal molecular signatures associated with recombinant adeno‐associated virus production in HEK293 cells	3
Corrections to: Soluble production and function of vascular endothelial growth factor/basic fibroblast growth factor complex peptide	3
Exploring the biocatalysis of psilocybin and other tryptamines: Enzymatic pathways, synthetic strategies, and industrial implications	3
Analytical characterization of host‐cell‐protein‐rich aggregates in monoclonal antibody solutions	3

Accelerated CMC workflows to enable speed to clinic in the COVID‐19 era: A multi‐company view from the biopharmaceutical industry	3
High‐throughput in silico workflow for optimization and characterization of multimodal chromatographic processes	3
Biotransformation of maize bran‐derived ferulic acid to vanillin using an adapted strain of Amycolatopsis sp. ATCC 39116	3
Tangential flow filtration‐facilitated purification of human red blood cell membrane fragments and its preferential use in removing unencapsulated material from resealed red blood cell ghosts compared	3
Removal of phenolic inhibitors from lignocellulose hydrolysates using laccases for the production of fuels and chemicals	3
Model validation and selection in metabolic flux analysis and flux balance analysis	3
Rapid intensification of an established CHO cell fed‐batch process	3
Advances in polymer‐based cell encapsulation and its applications in tissue repair	3
Enhancement of D‐mannitol production by fine‐tuned expression of mannitol‐1‐phosphate dehydrogenase in Synechocystis sp. PCC6803	3
Optimization and feedback control system of dilution rate for 1,3‐propanediol in two‐stage fermentation: A theoretical study	3
Minimizing batch‐to‐batch variability of a live virus vaccine by process analytical technologies	3
Integration of Planova filters in manufacturing processes of biologicals improve the virus safety effectively: A review of publicly available data	3
Multi‐omics kinetic analysis of recombinant adeno‐associated virus production by plasmid transfection of HEK293 cells	3
Highly iterated palindrome 1 sequence improves Synechococcus sp. PCC 7002 transformation efficiencies in a homology‐ and methylation‐dependent manner	3
Modeling the effect of oxidative stress on Bordetella pertussis fermentations	3
Process intensification of the baculovirus expression vector system using a perfusion process with a low multiplicity of infection at high cell concentrations	3
Advancing multiproduct resin reuse for development and clinical manufacturing of an antibody‐based therapeutic	2
Issue Information	2
Correction to “Soluble production and function of vascular endothelial growth factor/basic fibroblast growth factor complex peptide”	2
Characterization of large transgene integrations in Chinese hamster ovary cells using a bioengineered mammalian transposase	2
Strategies to improve CHO cell culture performance: Targeted deletion of amino acid catabolism and apoptosis genes paired with growth inhibitor supplementation	2
Issue Information	2
Issue Information	2
Use of ultrafiltration/diafiltration for the processing of antisense oligonucleotides	2
Glass microspargers as effective frit spargers in single use bioreactors	2
Investigation of a failed DNA assay leads to identification of an unexpected contaminant in a commonly used chromatography buffer	2
Design and optimization of membrane chromatography for monoclonal antibody charge variant separation	2
Assessment of membrane‐based downstream purification processes as a replacement to traditional resin bead for monoclonal antibody purification	2
Issue Information	2
Application of a simple unstructured kinetic and cost of goods models to support T‐cell therapy manufacture	2
A comparison of different intensified upstream processes highlighting the advantage of WuXi Biologics' Ultra‐high Productivity platform (WuXiUPTM) in improved product quality and	2
Bio‐based composite granules with simultaneous biocontrol and phosphorus fertilization roles: Outcomes from a lab‐scale in vitro assessment	2
Host cell proteins in monoclonal antibody processing: Control, detection, and removal	2
Mesenchymal stem cells derived‐exosomes enhanced amniotic membrane extract promotes corneal keratocyte proliferation	2
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Mechanistic modeling, simulation, and optimization of mixed‐mode chromatography for an antibody polishing step	2
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Enhancing cryopreservation of human induced pluripotent stem cells: Bottom‐up versus conventional freezing geometry	2
Changes to culture pH and dissolved oxygen can enhance chimeric antigen receptor T‐cell generation and differentiation	2
Development of targeted gene delivery system based on liposome and PAMAM dendrimer functionalized with hyaluronic acid and TAT peptide: In vitro and in vivo studies	2
Efficient immobilized phospholipase A1 on Mo‐basing nanomaterials for enzymatic degumming	2
Implementation of mDoE‐methods to a microcarrier‐based expansion processes for mesenchymal stem cells	2
N‐1 semi‐continuous transient perfusion in shake flask for ultra‐high density seeding of CHO cell cultures in benchtop bioreactors	2
Issue Information	2
The pandemic and resilience for the future: AccBio 2021	2
Xanthan gum enhances peripheral blood CIK cells cytotoxicity in serum‐free medium	2
Evaluation of lentiviral vector stability and development of ion exchange purification processes	2
Methods comparison of two‐dimensional gel electrophoresis for host cell protein characterization	2
Identification of infectious viruses for risk‐based virus testing of CHO unprocessed bulk using next‐generation sequencing	2
Digital application for drug product potency target evaluation in biopharmaceutical manufacturing	2
Optimization of an artificial neural network topology using response surface methodology for microbial fuel cell power prediction	2
Recent trends in biotechnological production, engineering, and applications of lysophospholipases	2
A novel NCI‐H69V small cell lung cancer functional mini‐tumor model for future treatment screening applications	2
A digital shadow of CAR T cell expansion in a perfusion bioreactor: Informing optimal harvest times for autologous cell therapy	2
Dentin regeneration based on tooth tissue engineering: A review	2
Continuous CTC separation through a DEP‐based contraction–expansion inertial microfluidic channel	2