Biotechnology Progress

Article	Citations
Proteomics‐based method to comprehensively model the removal of host cell protein impurities	76
Issue Information	55
Pre‐folding purification procedures for inclusion body‐derived non‐tagged cationic recombinant proteins with multiple disulfide bonds for efficient refolding	46
Dynamic pH profiles drive higher cell‐specific and volumetric productivity	45
Exploring the design space for T riton X ‐100 substitutes in viral inactivation applications	42
Acetate accumulation and regulation by process parameters control in Chinese hamster ovary cell culture	28
Optimized pulse‐feeding fed‐batch fermentation for enhanced lignin to polyhydroxyalkanoate transformation	26
Depth filtration for clarification of intensified lentiviral vector suspension cell culture	24
Dynamic flux balance analysis of 1,3‐propanediol production by clostridium butyricum fermentation	24
From protein structure to an optimized chromatographic capture step using multiscale modeling	23
The effects of the chemical modification on immobilized lipase features are affected by the enzyme crowding in the support	23
High‐yield recombinant adeno‐associated viral vector production by multivariate optimization of bioprocess and transfection conditions	22
Removal of chromatin by salt‐tolerant endonucleases for production of recombinant measles virus	21
The production of succinate with more CO 2 fixation reactions facilitate	20
Online monitored characterization of Phocaeicola vulgatus for organic acid production using anaerobic microtiter plate cultivations	19
Issue Information	19
Enrichment of full AAV capsids by preparative strong anion exchange chromatography	18
Evaluating multiproduct chromatography Protein A resin reuse for monoclonal antibodies in biopharmaceutical manufacturing	17
Genetically engineered neural stem cells (NSCs) therapy for neurological diseases; state‐of‐the‐art	17
Current status of platelet manufacturing in 3D or bioreactors	17
Correction to “Investigation of the hepatic respiration and liver zonation on rat hepatocytes using an integrated oxygen biosensor in a microscale device”	16
Issue Information	15
Development and application of an analytical approach to assess an antibody's potential for disulfide reduction	15
	15
A novel modified culture medium for enhancing redifferentiation of chondrocytes for cartilage tissue engineering applications	15

Immobilized enzymes as potent antibiofilm agent	15
Copper impurity of iron raw material contributes to improved cell culture performance	15
Hands‐free from inoculation to harvest: Microbial fermentation with multivariate model to automate induction of recombinant protein expression	14
Development of virus‐like particles‐based vaccines against coronaviruses	14
Wound dressing based on PVA nanofiber containing silk fibroin modified with GO /	14
Correlation of microbial community structure and volatile flavor compounds during corn yellow wine fermentation	14
Unraveling the potential and constraints associated with corn steep liquor as a nutrient source for industrial fermentations	13
Automation of an integrated micro‐scale platform for monoclonal antibody process development by incorporation of a depth filter mimic	13
Modification of microstructure and selected physicochemical properties of bacterial cellulose produced by bacterial isolate using hydrocolloid‐fortified Hestrin–Schramm	13
Enhancing CHO cell recombinant protein production using a perfusion‐directed host evolution approach	13
Raman spectroscopy and one‐dimensional convolutional neural network modeling as a real‐time monitoring tool for in vitro transaminase‐catalyzed synthesis of a pharmaceutically relevant amine precursor	13
The development of an automated microscope image tracking and analysis system	13
Enhanced production of isobutyl and isoamyl acetate using Yarrowia lipolytica	12
Petroleum coke supplementation for enhanced biogas production and phosphate removal under mesophilic conditions	12
Bioburden detection on surface and water samples in a rapid, ultra‐sensitive and high‐throughput manner	12
Characterization of lipoprotein supplement and influence of its oxidized lipid content on cell culture performance and monoclonal antibody production by a SP2	12
Antibody glycation during a Chinese hamster ovary fed‐batch process is following a constrained second order reaction	11
Impact of X ‐ray irradiation as an equivalent alternative to gamma for sterilization of single‐use bioprocessing polymers	11
Non‐sterile cultivation of Yarrowia lipolytica in fed‐batch mode for the production of lipids and biomass	11
Bioengineered chimeric tRNA / pre‐miRNAs as prodrugs in cancer therapy	11
Improved endotoxin removal using ecofriendly detergents for intensified plasmid capture	11
Improving trans‐cinnamic acid production in a model cyanobacterium	11
Shear stress and microRNAs for better metastatic cancer management	10
Primary clarification of mammalian cell culture fluid using enhanced sedimentation on inclined surfaces inside the single‐use disposable Sudhin BioSettler150	10
Effect of light fluctuations on photosynthesis and metabolic flux in Synechocystis sp. PCC 6803	10
Combining regulated and constitutive protein expression significantly boosts protein expression by increasing productivity without affecting CHO cell	10
A simple electrochemical genosensor based on polythiophene acetic acid film for detection of Schistosoma mansoni	10
When will we have a clone? An industry perspective on the typical CLD timeline	10
Surface decoration of low molecular weight polyethylenimine ( LMW PEI ) by phthalated dextrin for improved delivery of interleukin‐12 plasmid	10
A systemic approach to identifying sequence frameworks that decrease mAb production in a transient Chinese hamster ovary cel	9
General strategies for IgG‐like bispecific antibody purification	8
Use of single analytic tool to quantify both absolute N‐glycosylation and glycan distribution in monoclonal antibodies	8
Enhancing cell separation in a hybrid spiral dielectrophoretic microchannel: Numerical insights and optimal operating conditions	8
Accelerating IND‐enabling toxicology studies using protein products from stable pools or pools of clones in Chinese hamster ovary cells	8
Issue Information	8
Non‐thermal plasma decontamination of microbes: a state of the art	8
Proliferation and differentiation of primary bovine myoblasts using Chlorella vulgaris extract for sustainable production of cultured meat	8
Modeling scalability of impurity precipitation in downstream biomanufacturing	7
High‐sensitivity real‐time monitoring of pH and respiration activity unveils metabolic dynamics in shake flask cultures	7
Predictive modeling of single pass tangential flow filtration for continuous biomanufacturing	7
An adaptive modeling approach using spiking‐augmentation method to improve chemometric model performance in bioprocess monitoring	7
Metabolic engineering of rapidly growing Synechococcus elongatus strains for phototrophic producti	7
Issue Information	7
Comparing in silico flowsheet optimization strategies in biopharmaceutical downstream processes	7
Amino acid abundance and composition in cell culture medium affects trace metal tolerance and cholesterol synthesis	7
RETRACTION : Comparison of Expression Optimization of New Derivative of staphylokinase ( SAK ‐	7
	7
Comparison of multi‐column chromatography configurations through model‐based optimization	7
Transmission of poly( dT 60 )	7
Biotechnological potential of yeast cell wall: An overview	7

Cation exchange as a single polishing step for conjugated peptides	7
Online measurement of the viscosity in shake flasks enables monitoring of γ‐PGA production in depolymerase knockout mutants of	7
Optimization of carotenoid production by Umbelopsis ramanniana	7
Optimization of amino acid composition in CHO cell perfusion medium using definitive screening design and	7
A strategy for successful dual‐species protein expression of genes with non‐optimal codon usage destined for bacterial and yeast cell factories	6
Artificial intelligence and machine learning‐assisted digital applications for biopharmaceutical manufacturing	6
Process optimization mitigated the retention loss of an Fc‐fusion protein during ultrafiltration/diafiltration	6
Transcriptomic functional characterization of recombinant adeno‐associated virus producing cell line adapted to suspension‐growth	6
Effect of cell retention techniques in Komagataella phaffii lab‐scale continuous processes	6
A new method to immobilize urease in silk fibroin membrane by unidirectional nanopore dehydration	6
New technical concept for alternating tangential flow filtration in biotechnological cell separation processes	6
Hybrid modeling for in silico optimization of a dynamic perfusion cell culture process	6
Issue Information	6
Size‐based analysis of virus removal filter fouling using fractionated protein aggregates	6
Identification of compendial nonionic detergents for the replacement of Triton X‐100 in bioprocessing	6
Intensified perfusion culture (IPC) reduced recombinant protein fragmentation	6
A novel synthetic sRNA promoting protein overexpression in cell‐free systems	6
Continuous glucose feedback control using Raman spectroscopy and deep learning models for biopharmaceutical processes	6
Exploring the potential of machine learning for more efficient development and production of biopharmaceuticals	6
Impact of bioreactor process parameters and yeast biomass on Raman spectra	6
A systematic approach to reduce intraocular pressure for the treatment of glaucoma	6
Issue Information	6
Issue Information	5
Application of fucosylation inhibitors for production of afucosylated antibody	5
Synthetic modeling: A cell‐free approach for faster implementation of Raman spectroscopy in cell culture	5
Utilizing C ole– C ole parameters for in‐line feedback: Cell culture process adjustments based on	5
Modulation of nutrient precursors for controlling metabolic inhibitors by genome‐scale flux balance analysis	5
Design of a bactericidal hydrogel scaffold containing genipin crosslinked HF ‐18 peptide	5
Harnessing cell aggregates for enhanced adeno‐associated virus manufacturing: Cultivation strategies and scale‐up considerations	5
Antibody charge variant modulation by in vitro enzymatic treatment in different Chinese hamster ovary cell cultures	5
Application of a fluorescent dye‐based microfluidic sensor for real‐time detection of mAb aggregates	5
Integrated approach for purification of uricase and protease from Bacillus licheniformis by TPP and	5
Efficient optimization of time‐varying inputs in a fed‐batch cell culture process using design of dynamic experiments	5
Polyhydroxyalkanoates and exopolysaccharides: An alternative for valuation of the co‐production of microbial biopolymers	5
Purification of antisense oligonucleotides using hydrophobic interaction chromatography	5
Pharmaceutical proteins at the interfaces and the role of albumin	5
A synthetic platform for developing recombinant adeno‐associated virus type 8 producer cell lines	5
Production of 8,11‐dihydroxy fatty acids from oleic and palmitoleic acids by Escherichia coli cells expressing variant 6,8‐linoleate diol synthases from	5
Enhanced cell‐specific productivity through delayed supplementation of antioxidants in intensified processes	5
Chitosan based hybrid superabsorbent for controlled drug delivery application	5
Triton CG‐110 as an alternative for cell lysis in manufacturing of adeno‐associated virus‐based gene therapy	5
Special issue on integrated continuous biomanufacturing	5
Effects of LEDs, macronutrients and culture conditions on biomass and artemisinin production using Artemisia annuaL. suspension cultures	5
Modification of C‐segment of Cfa DnaE split intein for improving	5
Predictive mechanistic model for separation of monoclonal antibody, fab fragment, and aggregate species on multimodal chromatography	4
Adapting virus filtration to continuous processing: Effects of product and process variability on filtration performance	4
Application of Ambr15 system for simulation of entire SARS‐CoV ‐2 vaccine production process involving macrocarriers	4
Application of inline variable pathlength technology for rapid determination of dynamic binding capacity in downstream process development of biopharmaceuticals	4
Model validation and selection in metabolic flux analysis and flux balance analysis	4
Optimizing monoclonal antibody biosimilar production via transfer and active learning for targeted quality profiles	4
Role of membrane structure on the filtrate flux during monoclonal antibody filtration through virus retentive membranes	4
Cryopreservation practices in clinical and preclinical iPSC‐based cell therapies: Current challenges and future directions	4
Difficult‐to‐express antigen generation through a co‐expression and disassociation methodology	4
Development of multiparametric bioprinting method for generation of 3D printed cell‐laden structures	4
Simplifying stable CHO cell line generation with high probability of monoclonality by using microfluidic dispensing as an alternative to fluorescence	4
Effect of flux and shear rate on E. coli recovery in tangential flow filtration through a single hollow fiber	4
The combination of oleic acid, linoleic acid, palmitoleic acid, and α‐linolenic acid promoted the expansion of NK ‐92 cells in vitro	4
Real‐time online monitoring of insect cell proliferation and baculovirus infection using digital differential holographic microscopy and machine learning	4
Relationship between intracellular protein denaturation and irreversible inactivation of Saccharomyces pastorianus by low‐pressure carbon dioxide microbu	4
Proliferation of mammalian cells with Chlorococcum littorale algal compounds without serum support	4
Isoelectric point ( pI )‐based phase separation ( pI‐BPS ) purification of elastin‐like polypeptid	4
Environmental conditions during glycerol bioconversion affect 3‐hydroxypropionic acid bioproduction by Limosilactobacillus reuteri DSM	4
A low‐cost, high‐throughput microfluidic nano‐culture platform for functional metagenomics	4
Impedimetric nanoimmunosensor platform for aflatoxin B1 detection in peanuts	4
Effects of green light supplementation with red and blue combinations of LED light spectrums on the growth and transcriptional response of	4
Just passing through: Deploying aquaporins in microbial cell factories	4
Effect of adenosine and cordycepin on recombinant antibody production yields in two different Chinease hamster ovary cell lines	4
Issue Information	4
Aptamer‐functionalized quantum dots as theranostic nanotools against cancer and bacterial infections: A comprehensive overview of recent trends	4
Managing integrated continuous bioprocesses in real time: Deviations in product quality	4
Multi‐omics kinetic analysis of recombinant adeno‐associated virus production by plasmid transfection of HEK293 cells	3
Transcriptomic features reveal molecular signatures associated with recombinant adeno‐associated virus production in HEK293 cells	3
An updated overview of the application of CAR‐T cell therapy in neurological diseases	3
Sources and methods of manufacturing xanthan by fermentation of various carbon sources	3
Issue Information	3
Optimization of an artificial neural network topology using response surface methodology for microbial fuel cell power prediction	3
Issue Information	3
Development of chondrocyte‐laden alginate hydrogels with modulated microstructure and properties for cartilage regeneration	3
Design and optimization of membrane chromatography for monoclonal antibody charge variant separation	3
Minimizing batch‐to‐batch variability of a live virus vaccine by process analytical technologies	3

Development of a miniature bioreactor model to study the impact of pH and DOT fluctuations on	3
Efficient immobilized phospholipase A1 on Mo‐basing nanomaterials for enzymatic degumming	3
An automated high inoculation density fed‐batch bioreactor, enabled through N‐1 perfusion, accommodates clonal diversity and doubles titers	3
Process intensification of the baculovirus expression vector system using a perfusion process with a low multiplicity of infection at high cell conce	3
Evaluating end‐to‐end continuous antibody manufacture with column‐free capture alternatives from economic, environmental, and robustness perspectives	3
Modeling the effect of oxidative stress on Bordetella pertussis fermentations	3
Accelerated CMC workflows to enable speed to clinic in the COVID ‐19 era: A multi‐company view fro	3
Correction to “Soluble production and function of vascular endothelial growth factor/basic fibroblast growth factor complex peptide”	3
A novel NCI‐H69V small cell lung cancer functional mini‐tumor model for future treatment screening applications	3
Exploring the biocatalysis of psilocybin and other tryptamines: Enzymatic pathways, synthetic strategies, and industrial implications	3
Glass microspargers as effective frit spargers in single use bioreactors	3
High‐throughput in silico workflow for optimization and characterization of multimodal chromatographic processes	3
Advances in polymer‐based cell encapsulation and its applications in tissue repair	3
Analytical characterization of host‐cell‐protein‐rich aggregates in monoclonal antibody solutions	3
Highly iterated palindrome 1 sequence improves Synechococcus sp. PCC 7002 transformation efficiencies	3
Removal of phenolic inhibitors from lignocellulose hydrolysates using laccases for the production of fuels and chemicals	3
Tangential flow filtration‐facilitated purification of human red blood cell membrane fragments and its preferential use in removing unencapsulated material from resealed red blood cell ghosts compared	3
Comprehensive studies on building a scalable downstream process for mRNAs to enable mRNA therapeut	3
Enhancement of D‐mannitol production by fine‐tuned expression of mannitol‐1‐phosphate dehydrogenase in Synechocystis sp. PCC6803	3
Changes to culture pH and dissolved oxygen can enhance chimeric antigen receptor T‐cell generation and differentiation	3
Cover	3
Biotransformation of maize bran‐derived ferulic acid to vanillin using an adapted strain of Amycolatopsis sp. ATCC 39116	3
	3
Corrections to: Soluble production and function of vascular endothelial growth factor/basic fibroblast growth factor complex peptide	3
Integrated micro‐scale protein a chromatography and Low pH viral inactivation unit operations on an automated platform	3
Integration of Planova filters in manufacturing processes of biologicals improve the virus safety effectively: A review of publicly available data	3
Real time on‐line amino acid analysis to explore amino acid trends and link to glycosylation outcomes of a model monoclonal antibody	3
In silico optimization of a challenging bispecific antibody chromatography step	2
Enhancing the performance of an in vitro RNA biosensor through iterative design of experiments	2
Continuous CTC separation through a DEP‐based contraction–expansion inertial microfluidic channel	2
Use of ultrafiltration/diafiltration for the processing of antisense oligonucleotides	2
Effect of pH, NaCl concentration, and mAb concentration of feed solution on the filterability of Planova™ 20N and Planova™ BioEX	2
Secondary feed filtration and storage conditions influence trace element availability and process performance at 2000  L scale	2
A deep‐well plate enabled automated high‐throughput cell line development platform	2
Implementation of mDoE ‐methods to a microcarrier‐based expansion processes for mesenchymal stem cells	2
Advancing multiproduct resin reuse for development and clinical manufacturing of an antibody‐based therapeutic	2
Recent trends in biotechnological production, engineering, and applications of lysophospholipases	2
Efficient fermentative production of lactodifucotetraose by controlling sequential glycosyltransferase reactions in Escherichia coli	2
	2
Strategies to improve CHO cell culture performance: Targeted deletion of amino acid catabolism and apoptosis genes paired with growth inhibitor suppl	2
Development of targeted gene delivery system based on liposome and PAMAM dendrimer functionalized with hyaluronic acid and T	2
Smart process development: Application of machine‐learning and integrated process modeling for inclusion body purification processes	2
Modulation of the cell cycle and inhibition of histone deacetylases by small molecules increase recombinant adeno‐associated virus productivity across different HEK293 cell lines	2
Advanced strategies in glycosylation prediction and control during biopharmaceutical development: Avenues toward industry 4.0	2
Dentin regeneration based on tooth tissue engineering: A review	2
Mesenchymal stem cells derived‐exosomes enhanced amniotic membrane extract promotes corneal keratocyte proliferation	2
Understanding the effect of temperature downshift on CHO cell growth, antibody titer and product quality by intracellular metabolite profiling and in	2
A digital shadow of CAR T cell expansion in a perfusion bioreactor: Informing optimal harvest times for autologous cell therapy	2
Issue Information	2
Issue Information	2
A modular and multi‐functional purification strategy that enables a common framework for manufacturing scale integrated and continuous biomanufacturing	2
Evaluation of lentiviral vector stability and development of ion exchange purification processes	2
Mechanistic modeling, simulation, and optimization of mixed‐mode chromatography for an antibody polishing step	2
Identification of infectious viruses for risk‐based virus testing of CHO unprocessed bulk using next‐generation sequencing	2
Enhancing cryopreservation of human induced pluripotent stem cells: Bottom‐up versus conventional freezing geometry	2
A comparison of different intensified upstream processes highlighting the advantage of WuXi Biologics' Ultra‐high Productivity platform ( WuXiUP	2
5‐Aza combined with VPA reprograms human T lineage acute leukemia Jurkat cells into B‐cell‐like cells by epigenetic activation of PAX5	2
Xanthan gum enhances peripheral blood CIK cells cytotoxicity in serum‐free medium	2
Examining organic acid production potential and growth‐coupled strategies in Issatchenkia orientalis using constraint‐based modeling	2
Characterization of large transgene integrations in Chinese hamster ovary cells using a bioengineered mammalian transposase	2
N‐1 semi‐continuous transient perfusion in shake flask for ultra‐high density seeding of CHO cell cultures in benchtop bioreactors	2
Image analysis method for measurement and prediction of intra‐matrix IgG diffusion	2
“Just add small molecules” cell‐free protein synthesis: Combining DNA template and cell extract preparation into a single fermentation	2
Investigation of a failed DNA assay leads to identification of an unexpected contaminant in a commonly used chromatography buffer	2
Bio‐based composite granules with simultaneous biocontrol and phosphorus fertilization roles: Outcomes from a lab‐scale in vitro assessment	2
Digital application for drug product potency target evaluation in biopharmaceutical manufacturing	2
Host cell proteins in monoclonal antibody processing: Control, detection, and removal	2
Assessment of membrane‐based downstream purification processes as a replacement to traditional resin bead for monoclonal antibody purification	2
Methods comparison of two‐dimensional gel electrophoresis for host cell protein characterization	2