OOIR: Observatory of International Research

Papers

(The TQCC of Biotechnology Progress is 5. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-08-01 to 2025-08-01.)

Article	Citations
Issue Information	101
Depth filtration for clarification of intensified lentiviral vector suspension cell culture	69
From protein structure to an optimized chromatographic capture step using multiscale modeling	65
Retrospective assessment of clonality of a legacy cell line by analytical subcloning of the master cell bank	50
Dynamic flux balance analysis of 1,3‐propanediol production by clostridium butyricum fermentation	39
Pre‐folding purification procedures for inclusion body‐derived non‐tagged cationic recombinant proteins with multiple disulfide bonds for efficient refolding	37
Optimized pulse‐feeding fed‐batch fermentation for enhanced lignin to polyhydroxyalkanoate transformation	31
Acetate accumulation and regulation by process parameters control in Chinese hamster ovary cell culture	27
The effects of the chemical modification on immobilized lipase features are affected by the enzyme crowding in the support	26
Proteomics‐based method to comprehensively model the removal of host cell protein impurities	25
Establishment and characterization of lung co‐culture spheroids for paclitaxel loaded Eudragit® RL 100 nanoparticle evaluation	22
Exometabolome profiling reveals activation of the carnitine buffering pathway in fed‐batch cultures ofCHOcells co‐fed with glucose and lactic acid	21
Evaluating multiproduct chromatography Protein A resin reuse for monoclonal antibodies in biopharmaceutical manufacturing	20
High‐yield recombinant adeno‐associated viral vector production by multivariate optimization of bioprocess and transfection conditions	20
The production of succinate with more CO₂ fixation reactions facilitated by RuBisCO‐based engineered Escherichia coli	20
Current status of platelet manufacturing in 3D or bioreactors	19
Online monitored characterization of Phocaeicola vulgatus for organic acid production using anaerobic microtiter plate cultivations	17
Genetically engineered neural stem cells (NSCs) therapy for neurological diseases; state‐of‐the‐art	16
Removal of chromatin by salt‐tolerant endonucleases for production of recombinant measles virus	16
Issue Information	16
	15
Unraveling the potential and constraints associated with corn steep liquor as a nutrient source for industrial fermentations	15
Development and application of an analytical approach to assess an antibody's potential for disulfide reduction	15
A novel modified culture medium for enhancing redifferentiation of chondrocytes for cartilage tissue engineering applications	15
Copper impurity of iron raw material contributes to improved cell culture performance	15

Modification of microstructure and selected physicochemical properties of bacterial cellulose produced by bacterial isolate using hydrocolloid‐fortified Hestrin–Schramm medium	15
Wound dressing based on PVA nanofiber containing silk fibroin modified with GO/ZnO nanoparticles for superficial wound healing: In vitro and in vivo evaluations	14
Issue Information	14
Immobilized enzymes as potent antibiofilm agent	14
Raman spectroscopy and one‐dimensional convolutional neural network modeling as a real‐time monitoring tool for in vitro transaminase‐catalyzed synthesis of a pharmaceutically relevant amine precursor	13
Impact of X‐ray irradiation as an equivalent alternative to gamma for sterilization of single‐use bioprocessing polymers	13
Development of virus‐like particles‐based vaccines against coronaviruses	13
Bioburden detection on surface and water samples in a rapid, ultra‐sensitive and high‐throughput manner	13
Correction to “Investigation of the hepatic respiration and liver zonation on rat hepatocytes using an integrated oxygen biosensor in a microscale device”	13
Correlation of microbial community structure and volatile flavor compounds during corn yellow wine fermentation	13
Non‐sterile cultivation of Yarrowia lipolytica in fed‐batch mode for the production of lipids and biomass	13
Petroleum coke supplementation for enhanced biogas production and phosphate removal under mesophilic conditions	12
The development of an automated microscope image tracking and analysis system	12
Improved endotoxin removal using ecofriendly detergents for intensified plasmid capture	12
Antibody glycation during a Chinese hamster ovary fed‐batch process is following a constrained second order reaction	12
Characterization of lipoprotein supplement and influence of its oxidized lipid content on cell culture performance and monoclonal antibody production by a SP2/0 hybridoma cell line	12
Hypoxia preconditioned mesenchymal stem cell‐derived exosomes induce ex vivo expansion of umbilical cord blood hematopoietic stem cells CD133+ by stimulation of Notch signaling pathway	11
When will we have a clone? An industry perspective on the typical CLD timeline	11
Shear stress and microRNAs for better metastatic cancer management	11
Primary clarification of mammalian cell culture fluid using enhanced sedimentation on inclined surfaces inside the single‐use disposable Sudhin BioSettler150	11
Enhanced production of isobutyl and isoamyl acetate using Yarrowia lipolytica	11
General strategies for IgG‐like bispecific antibody purification	11
A systemic approach to identifying sequence frameworks that decrease mAb production in a transient Chinese hamster ovary cell expression system	10
Enhancing cell separation in a hybrid spiral dielectrophoretic microchannel: Numerical insights and optimal operating conditions	10
Combining regulated and constitutive protein expression significantly boosts protein expression by increasing productivity without affecting CHO cell growth	10
Surface decoration of low molecular weight polyethylenimine (LMW PEI) by phthalated dextrin for improved delivery of interleukin‐12 plasmid	10
Hybrid fusion protein as a dual protease inhibitor for the healing of chronic wounds	10
A simple electrochemical genosensor based on polythiophene acetic acid film for detection of Schistosoma mansoni	9
Bioengineered chimeric tRNA/pre‐miRNAs as prodrugs in cancer therapy	9
Non‐thermal plasma decontamination of microbes: a state of the art	9
Improving trans‐cinnamic acid production in a model cyanobacterium	9
Comparing in silico flowsheet optimization strategies in biopharmaceutical downstream processes	9
Accelerating IND‐enabling toxicology studies using protein products from stable pools or pools of clones in Chinese hamster ovary cells	9
Proliferation and differentiation of primary bovine myoblasts using Chlorella vulgaris extract for sustainable production of cultured meat	9
Effect of light fluctuations on photosynthesis and metabolic flux in Synechocystis sp. PCC 6803	9
Transmission of poly(dT₆₀) single‐stranded DNA through polycarbonate track‐etched ultrafiltration membranes	8
High‐sensitivity real‐time monitoring of pH and respiration activity unveils metabolic dynamics in shake flask cultures	8
Metabolic engineering of rapidly growing Synechococcus elongatus strains for phototrophic production of alkanes	8
Issue Information	8
Issue Information	8
Use of single analytic tool to quantify both absolute N‐glycosylation and glycan distribution in monoclonal antibodies	8
Modeling scalability of impurity precipitation in downstream biomanufacturing	8
Comparison of multi‐column chromatography configurations through model‐based optimization	8
Amino acid abundance and composition in cell culture medium affects trace metal tolerance and cholesterol synthesis	8
Predictive modeling of single pass tangential flow filtration for continuous biomanufacturing	7
Cation exchange as a single polishing step for conjugated peptides	7
Metabolic trends of Chinese hamster ovary cells in biopharmaceutical production under batch and fed‐batch conditions	7
Biotechnological potential of yeast cell wall: An overview	7
An adaptive modeling approach using spiking‐augmentation method to improve chemometric model performance in bioprocess monitoring	7
Hybrid modeling for in silico optimization of a dynamic perfusion cell culture process	7

New technical concept for alternating tangential flow filtration in biotechnological cell separation processes	7
	7
Online measurement of the viscosity in shake flasks enables monitoring of γ‐PGA production in depolymerase knockout mutants of Bacillus subtilis with the phosphate‐starvation inducib	7
Optimization of carotenoid production by Umbelopsis ramanniana	7
A novel synthetic sRNA promoting protein overexpression in cell‐free systems	7
Continuous glucose feedback control using Raman spectroscopy and deep learning models for biopharmaceutical processes	6
Issue Information	6
Harnessing cell aggregates for enhanced adeno‐associated virus manufacturing: Cultivation strategies and scale‐up considerations	6
Modification of C‐segment of CfaDnaE split intein for improving clean‐in‐place in chromatography process	6
PVA based nanofiber containing cellulose modified with graphitic carbon nitride/nettles/trachyspermum accelerates wound healing	6
Issue Information	6
Size‐based analysis of virus removal filter fouling using fractionated protein aggregates	6
Exploring the potential of machine learning for more efficient development and production of biopharmaceuticals	6
A new method to immobilize urease in silk fibroin membrane by unidirectional nanopore dehydration	6
Antibody charge variant modulation by in vitro enzymatic treatment in different Chinese hamster ovary cell cultures	6
Process optimization mitigated the retention loss of an Fc‐fusion protein during ultrafiltration/diafiltration	6
A strategy for successful dual‐species protein expression of genes with non‐optimal codon usage destined for bacterial and yeast cell factories	6
Identification of compendial nonionic detergents for the replacement of Triton X‐100 in bioprocessing	6
A systematic approach to reduce intraocular pressure for the treatment of glaucoma	6
Modulation of nutrient precursors for controlling metabolic inhibitors by genome‐scale flux balance analysis	6
A synthetic platform for developing recombinant adeno‐associated virus type 8 producer cell lines	6
Polyhydroxyalkanoates and exopolysaccharides: An alternative for valuation of the co‐production of microbial biopolymers	6
Impact of bioreactor process parameters and yeast biomass on Raman spectra	6
Transcriptomic functional characterization of recombinant adeno‐associated virus producing cell line adapted to suspension‐growth	6
Efficient optimization of time‐varying inputs in a fed‐batch cell culture process using design of dynamic experiments	5
Effects of LEDs, macronutrients and culture conditions on biomass and artemisinin production using Artemisia annuaL. suspension cultures	5
Special issue on integrated continuous biomanufacturing	5
Simplifying stable CHO cell line generation with high probability of monoclonality by using microfluidic dispensing as an alternative to fluorescence activated cell sorting	5
Isoelectric point (pI)‐based phase separation (pI‐BPS) purification of elastin‐like polypeptides (ELPs) containing charged, biologically active fusion proteins (E	5
A low‐cost, high‐throughput microfluidic nano‐culture platform for functional metagenomics	5
Intensified perfusion culture (IPC) reduced recombinant protein fragmentation	5
Design of a bactericidal hydrogel scaffold containing genipin crosslinked HF‐18 peptide	5
Chitosan based hybrid superabsorbent for controlled drug delivery application	5
Synthetic modeling: A cell‐free approach for faster implementation of Raman spectroscopy in cell culture	5
Enhanced cell‐specific productivity through delayed supplementation of antioxidants in intensified processes	5
Application of fucosylation inhibitors for production of afucosylated antibody	5
Issue Information	5
Pharmaceutical proteins at the interfaces and the role of albumin	5
A ribonucleoprotein‐based decaplex CRISPR/Cas9 knockout strategy for CHO host engineering	5
The effect of biosynthesized selenium nanoparticles on the expression of CYP51A and HSP90 antifungal resistance genes in Aspergillus fumigatus and Aspergi	5
Adapting virus filtration to continuous processing: Effects of product and process variability on filtration performance	5
Application of a fluorescent dye‐based microfluidic sensor for real‐time detection of mAb aggregates	5
Production of 8,11‐dihydroxy fatty acids from oleic and palmitoleic acids by Escherichia coli cells expressing variant 6,8‐linoleate diol synthases from Penicillium oxalicum	5