OOIR: Observatory of International Research

Papers

(The TQCC of Biotechnology Progress is 5. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2022-01-01 to 2026-01-01.)

Article	Citations
Proteomics‐based method to comprehensively model the removal of host cell protein impurities	76
Issue Information	55
Pre‐folding purification procedures for inclusion body‐derived non‐tagged cationic recombinant proteins with multiple disulfide bonds for efficient refolding	46
Dynamic pH profiles drive higher cell‐specific and volumetric productivity	45
Exploring the design space for T riton X ‐100 substitutes in viral inactivation applications	42
Acetate accumulation and regulation by process parameters control in Chinese hamster ovary cell culture	28
Optimized pulse‐feeding fed‐batch fermentation for enhanced lignin to polyhydroxyalkanoate transformation	26
Dynamic flux balance analysis of 1,3‐propanediol production by clostridium butyricum fermentation	24
Depth filtration for clarification of intensified lentiviral vector suspension cell culture	24
The effects of the chemical modification on immobilized lipase features are affected by the enzyme crowding in the support	23
From protein structure to an optimized chromatographic capture step using multiscale modeling	23
High‐yield recombinant adeno‐associated viral vector production by multivariate optimization of bioprocess and transfection conditions	22
Removal of chromatin by salt‐tolerant endonucleases for production of recombinant measles virus	21
The production of succinate with more CO ₂ fixation reactions facilitate	20
Online monitored characterization of Phocaeicola vulgatus for organic acid production using anaerobic microtiter plate cultivations	19
Issue Information	19
Enrichment of full AAV capsids by preparative strong anion exchange chromatography	18
Evaluating multiproduct chromatography Protein A resin reuse for monoclonal antibodies in biopharmaceutical manufacturing	17
Genetically engineered neural stem cells (NSCs) therapy for neurological diseases; state‐of‐the‐art	17
Current status of platelet manufacturing in 3D or bioreactors	17
Correction to “Investigation of the hepatic respiration and liver zonation on rat hepatocytes using an integrated oxygen biosensor in a microscale device”	16
Issue Information	15
Development and application of an analytical approach to assess an antibody's potential for disulfide reduction	15
	15
A novel modified culture medium for enhancing redifferentiation of chondrocytes for cartilage tissue engineering applications	15

Immobilized enzymes as potent antibiofilm agent	15
Copper impurity of iron raw material contributes to improved cell culture performance	15
Hands‐free from inoculation to harvest: Microbial fermentation with multivariate model to automate induction of recombinant protein expression	14
Development of virus‐like particles‐based vaccines against coronaviruses	14
Wound dressing based on PVA nanofiber containing silk fibroin modified with GO /	14
Correlation of microbial community structure and volatile flavor compounds during corn yellow wine fermentation	14
Modification of microstructure and selected physicochemical properties of bacterial cellulose produced by bacterial isolate using hydrocolloid‐fortified Hestrin–Schramm	13
Enhancing CHO cell recombinant protein production using a perfusion‐directed host evolution approach	13
Raman spectroscopy and one‐dimensional convolutional neural network modeling as a real‐time monitoring tool for in vitro transaminase‐catalyzed synthesis of a pharmaceutically relevant amine precursor	13
The development of an automated microscope image tracking and analysis system	13
Unraveling the potential and constraints associated with corn steep liquor as a nutrient source for industrial fermentations	13
Automation of an integrated micro‐scale platform for monoclonal antibody process development by incorporation of a depth filter mimic	13
Petroleum coke supplementation for enhanced biogas production and phosphate removal under mesophilic conditions	12
Bioburden detection on surface and water samples in a rapid, ultra‐sensitive and high‐throughput manner	12
Characterization of lipoprotein supplement and influence of its oxidized lipid content on cell culture performance and monoclonal antibody production by a SP2	12
Enhanced production of isobutyl and isoamyl acetate using Yarrowia lipolytica	12
Impact of X ‐ray irradiation as an equivalent alternative to gamma for sterilization of single‐use bioprocessing polymers	11
Non‐sterile cultivation of Yarrowia lipolytica in fed‐batch mode for the production of lipids and biomass	11
Bioengineered chimeric tRNA / pre‐miRNAs as prodrugs in cancer therapy	11
Improved endotoxin removal using ecofriendly detergents for intensified plasmid capture	11
Improving trans‐cinnamic acid production in a model cyanobacterium	11
Antibody glycation during a Chinese hamster ovary fed‐batch process is following a constrained second order reaction	11
Shear stress and microRNAs for better metastatic cancer management	10
Primary clarification of mammalian cell culture fluid using enhanced sedimentation on inclined surfaces inside the single‐use disposable Sudhin BioSettler150	10
Effect of light fluctuations on photosynthesis and metabolic flux in Synechocystis sp. PCC 6803	10
Combining regulated and constitutive protein expression significantly boosts protein expression by increasing productivity without affecting CHO cell	10
A simple electrochemical genosensor based on polythiophene acetic acid film for detection of Schistosoma mansoni	10
When will we have a clone? An industry perspective on the typical CLD timeline	10
Surface decoration of low molecular weight polyethylenimine ( LMW PEI ) by phthalated dextrin for improved delivery of interleukin‐12 plasmid	10
A systemic approach to identifying sequence frameworks that decrease mAb production in a transient Chinese hamster ovary cel	9
General strategies for IgG‐like bispecific antibody purification	8
Use of single analytic tool to quantify both absolute N‐glycosylation and glycan distribution in monoclonal antibodies	8
Enhancing cell separation in a hybrid spiral dielectrophoretic microchannel: Numerical insights and optimal operating conditions	8
Accelerating IND‐enabling toxicology studies using protein products from stable pools or pools of clones in Chinese hamster ovary cells	8
Issue Information	8
Non‐thermal plasma decontamination of microbes: a state of the art	8
Proliferation and differentiation of primary bovine myoblasts using Chlorella vulgaris extract for sustainable production of cultured meat	8
Metabolic engineering of rapidly growing Synechococcus elongatus strains for phototrophic producti	7
Issue Information	7
Comparing in silico flowsheet optimization strategies in biopharmaceutical downstream processes	7
Amino acid abundance and composition in cell culture medium affects trace metal tolerance and cholesterol synthesis	7
RETRACTION : Comparison of Expression Optimization of New Derivative of staphylokinase ( SAK ‐	7
	7
Comparison of multi‐column chromatography configurations through model‐based optimization	7
Transmission of poly( dT ₆₀ )	7
Biotechnological potential of yeast cell wall: An overview	7
Cation exchange as a single polishing step for conjugated peptides	7
Online measurement of the viscosity in shake flasks enables monitoring of γ‐PGA production in depolymerase knockout mutants of	7
Optimization of carotenoid production by Umbelopsis ramanniana	7
Optimization of amino acid composition in CHO cell perfusion medium using definitive screening design and	7

Modeling scalability of impurity precipitation in downstream biomanufacturing	7
High‐sensitivity real‐time monitoring of pH and respiration activity unveils metabolic dynamics in shake flask cultures	7
Predictive modeling of single pass tangential flow filtration for continuous biomanufacturing	7
An adaptive modeling approach using spiking‐augmentation method to improve chemometric model performance in bioprocess monitoring	7
Transcriptomic functional characterization of recombinant adeno‐associated virus producing cell line adapted to suspension‐growth	6
Effect of cell retention techniques in Komagataella phaffii lab‐scale continuous processes	6
New technical concept for alternating tangential flow filtration in biotechnological cell separation processes	6
A new method to immobilize urease in silk fibroin membrane by unidirectional nanopore dehydration	6
Hybrid modeling for in silico optimization of a dynamic perfusion cell culture process	6
Issue Information	6
Size‐based analysis of virus removal filter fouling using fractionated protein aggregates	6
Identification of compendial nonionic detergents for the replacement of Triton X‐100 in bioprocessing	6
Intensified perfusion culture (IPC) reduced recombinant protein fragmentation	6
A novel synthetic sRNA promoting protein overexpression in cell‐free systems	6
Continuous glucose feedback control using Raman spectroscopy and deep learning models for biopharmaceutical processes	6
Exploring the potential of machine learning for more efficient development and production of biopharmaceuticals	6
Impact of bioreactor process parameters and yeast biomass on Raman spectra	6
A systematic approach to reduce intraocular pressure for the treatment of glaucoma	6
A strategy for successful dual‐species protein expression of genes with non‐optimal codon usage destined for bacterial and yeast cell factories	6
Issue Information	6
Artificial intelligence and machine learning‐assisted digital applications for biopharmaceutical manufacturing	6
Process optimization mitigated the retention loss of an Fc‐fusion protein during ultrafiltration/diafiltration	6
Harnessing cell aggregates for enhanced adeno‐associated virus manufacturing: Cultivation strategies and scale‐up considerations	5
Antibody charge variant modulation by in vitro enzymatic treatment in different Chinese hamster ovary cell cultures	5
Application of a fluorescent dye‐based microfluidic sensor for real‐time detection of mAb aggregates	5
Integrated approach for purification of uricase and protease from Bacillus licheniformis by TPP and	5
Efficient optimization of time‐varying inputs in a fed‐batch cell culture process using design of dynamic experiments	5
Polyhydroxyalkanoates and exopolysaccharides: An alternative for valuation of the co‐production of microbial biopolymers	5
Purification of antisense oligonucleotides using hydrophobic interaction chromatography	5
Pharmaceutical proteins at the interfaces and the role of albumin	5
A synthetic platform for developing recombinant adeno‐associated virus type 8 producer cell lines	5
Production of 8,11‐dihydroxy fatty acids from oleic and palmitoleic acids by Escherichia coli cells expressing variant 6,8‐linoleate diol synthases from	5
Enhanced cell‐specific productivity through delayed supplementation of antioxidants in intensified processes	5
Chitosan based hybrid superabsorbent for controlled drug delivery application	5
Triton CG‐110 as an alternative for cell lysis in manufacturing of adeno‐associated virus‐based gene therapy	5
Special issue on integrated continuous biomanufacturing	5
Effects of LEDs, macronutrients and culture conditions on biomass and artemisinin production using Artemisia annuaL. suspension cultures	5
Modification of C‐segment of Cfa DnaE split intein for improving	5
Issue Information	5
Application of fucosylation inhibitors for production of afucosylated antibody	5
Synthetic modeling: A cell‐free approach for faster implementation of Raman spectroscopy in cell culture	5
Utilizing C ole– C ole parameters for in‐line feedback: Cell culture process adjustments based on	5
Modulation of nutrient precursors for controlling metabolic inhibitors by genome‐scale flux balance analysis	5
Design of a bactericidal hydrogel scaffold containing genipin crosslinked HF ‐18 peptide	5