OOIR: Observatory of International Research

Papers

(The TQCC of Biotechnology Progress is 4. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-04-01 to 2025-04-01.)

Article	Citations
Characterization of large transgene integrations in Chinese hamster ovary cells using a bioengineered mammalian transposase	85
Development of targeted gene delivery system based on liposome and PAMAM dendrimer functionalized with hyaluronic acid and TAT peptide: In vitro and in vivo studies	65
Characterization of soy protein hydrolysates and influence of its iron content on monoclonal antibody production by a murine hybridoma cell line	58
Optimization of carotenoid production by Umbelopsis ramanniana	32
Issue Information	32
The pandemic and resilience for the future: AccBio 2021	30
Modulating and optimizing Pluronic F‐68 concentrations and feeding for intensified perfusion Chinese hamster ovary cell cultures	26
Issue Information	24
Valorizing prickly pear cladodes via methane‐arrested anaerobic digestion for carboxylic acid production	24
Acetate accumulation and regulation by process parameters control in Chinese hamster ovary cell culture	24
Methods comparison of two‐dimensional gel electrophoresis for host cell protein characterization	23
Scale‐up issues during sterile filtration of glycoconjugate vaccines	21
Issue Information	21
Relationship between intracellular protein denaturation and irreversible inactivation of Saccharomyces pastorianus by low‐pressure carbon dioxide microbubbles	19
Establishment and characterization of lung co‐culture spheroids for paclitaxel loaded Eudragit® RL 100 nanoparticle evaluation	18
Surfactants reduce aggregation of monoclonal antibodies in cell culture medium with improvement in performance of mammalian cell culture	17
Optimization and characterization of rhamnolipid production by Pseudomonas aeruginosa NY3 using waste frying oil as	16
Protective effects of non‐catalytic proteins on endoglucanase activity at air and lignin interfaces	15
Assessment of corn stover pretreated with shock and alkali using methane‐arrested anaerobic digestion	14
Retrospective assessment of clonality of a legacy cell line by analytical subcloning of the master cell bank	14
X‐ray sterilization of biopharmaceutical manufacturing equipment—Extractables profile of a film material and copolyester Tritan™ compared to gamma irradiation	13
Real‐time online monitoring of insect cell proliferation and baculovirus infection using digital differential holographic microscopy and machine learning	13
Assessing the impact of sanitization methods for regenerated cellulose ultrafiltration/diafiltration membrane on membrane integrity and protein quality	13
Online monitoring of the respiration activity in 96‐deep‐well microtiter plate Chinese hamster ovary cultures streamlines kill curve experiments	13
Genetic rearrangement during site specific integration event facilitates cell line development of a bispecific molecule	13

A novel synthetic sRNA promoting protein overexpression in cell‐free systems	13
New technical concept for alternating tangential flow filtration in biotechnological cell separation processes	12
Multivariate data analysis of process parameters affecting the growth and productivity of stable Chinese hamster ovary cell pools expressing SARS‐CoV‐2 spike protein as vaccine antigen in e	12
Deep brain stimulation: Challenges at the tissue‐electrode interface and current solutions	12
Cation exchange as a single polishing step for conjugated peptides	12
Development of a pan‐tau multivalent nanobody that binds tau aggregation motifs and recognizes pathological tau aggregates	12
Hybrid modeling for in silico optimization of a dynamic perfusion cell culture process	11
Dynamic flux balance analysis of 1,3‐propanediol production by clostridium butyricum fermentation	11
CHO stable pool fed‐batch process development of SARS‐CoV‐2 spike protein production: Impact of aeration conditions and feeding strategies	11
High‐yield recombinant adeno‐associated viral vector production by multivariate optimization of bioprocess and transfection conditions	11
From protein structure to an optimized chromatographic capture step using multiscale modeling	11
Detergent/surfactant retention during ultrafiltration in the formulation of biotherapeutics	10
Recent advances of dendrimer in targeted delivery of drugs and genes to stem cells as cellular vehicles	10
Effect of iron addition on mAb productivity and oxidative stress in Chinese hamster ovary culture	10
Depth filtration for clarification of intensified lentiviral vector suspension cell culture	10
Optimized pulse‐feeding fed‐batch fermentation for enhanced lignin to polyhydroxyalkanoate transformation	10
Chemistry of lignin and hemicellulose structures interacts with hydrothermal pretreatment severity and affects cellulose conversion	9
A comparison of different intensified upstream processes highlighting the advantage of WuXi Biologics' Ultra‐high Productivity platform (WuXiUP^TM) in improved product quality and	9
Proteomics‐based method to comprehensively model the removal of host cell protein impurities	9
Mesenchymal stem cells derived‐exosomes enhanced amniotic membrane extract promotes corneal keratocyte proliferation	8
Model validation and selection in metabolic flux analysis and flux balance analysis	8
An adaptive modeling approach using spiking‐augmentation method to improve chemometric model performance in bioprocess monitoring	8
Bio‐based composite granules with simultaneous biocontrol and phosphorus fertilization roles: Outcomes from a lab‐scale in vitro assessment	8
The effects of the chemical modification on immobilized lipase features are affected by the enzyme crowding in the support	8
Effect of adenosine and cordycepin on recombinant antibody production yields in two different Chinease hamster ovary cell lines	8
Pre‐folding purification procedures for inclusion body‐derived non‐tagged cationic recombinant proteins with multiple disulfide bonds for efficient refolding	8
Predictive modeling of single pass tangential flow filtration for continuous biomanufacturing	8
Activation of the PERK branch of the unfolded protein response during production reduces specific productivity in CHO cells via downregulation of PDGFRa and IRE1a	8
Evaluation of the efficiency of modified PAMAM dendrimer with low molecular weight protamine peptide to deliver IL ‐12 plasmid int	8
Exometabolome profiling reveals activation of the carnitine buffering pathway in fed‐batch cultures ofCHOcells co‐fed with glucose and lactic acid	8
Online measurement of the viscosity in shake flasks enables monitoring of γ‐PGA production in depolymerase knockout mutants of Bacillus subtilis with the phosphate‐starvation inducib	8
Metabolic trends of Chinese hamster ovary cells in biopharmaceutical production under batch and fed‐batch conditions	8
A Novel Polyculture Growth Model of Native Microalgal Communities to Estimate Biomass Productivity for Biofuel Production	8
Analysis of tradeoffs between purification factor and yield for high‐performance countercurrent membrane purification for protein separations	7
Process analytics 4.0: A paradigm shift in rapid analytics for biologics development	7
Process optimization mitigated the retention loss of an Fc‐fusion protein during ultrafiltration/diafiltration	7
Lignin degradation by microorganisms: A review	7
Engineering tumor constructs to study matrix‐dependent angiogenic signaling of breast cancer cells	7
Effect of nucleophilic additives on phosphoric acid pretreatment of lignocelluloses	7
CD133 ligand‐enhanced etoposide‐liposome complex for targeted killing of lung cancer cells	7
Real time on‐line amino acid analysis to explore amino acid trends and link to glycosylation outcomes of a model monoclonal antibody	7
Assessment of shock pretreatment and alkali pretreatment on corn stover using enzymatic hydrolysis	6
Evaluating multiproduct chromatography Protein A resin reuse for monoclonal antibodies in biopharmaceutical manufacturing	6
Reshaping cell line development and CMC strategy for fast responses to pandemic outbreak	6
Mechanistic insights into hypoxia‐induced metabolic reprogramming in colorectal cancer through genome‐scale modeling	6
Methods for identifying precipitates and improving stability of chemically defined highly concentrated cell culture media	6
Evaluation of single‐use tangential flow filtration technology for purification of activated polysaccharides used in conjugate vaccine manufacturing	6
pH and conductivity transients during elution of IgG from protein A columns	6
Current status of platelet manufacturing in 3D or bioreactors	6
Dentin regeneration based on tooth tissue engineering: A review	6

Issue Information	6
Xanthan gum enhances peripheral blood CIK cells cytotoxicity in serum‐free medium	6
Issue Information	6
Removal of chromatin by salt‐tolerant endonucleases for production of recombinant measles virus	6
Issue Information	5
Intramodule pressure profiles and protein accumulation during tangential flow filtration	5
Engineering a high‐throughput clone for industrial‐scale production of long‐acting GLP‐1 analogue with retained bio‐efficacy	5
Removal of phenolic inhibitors from lignocellulose hydrolysates using laccases for the production of fuels and chemicals	5
Issue Information	5
Proteomic analysis of host cell protein fouling during bioreactor harvesting	5
Probing morphological, genetic and metabolomic changes of in vitro embryo development in a microfluidic device	5
Fed‐batch performance profiles for mAb production using different intensified N − 1 seed strategies are CHO cell‐line dependent	5
Examining organic acid production potential and growth‐coupled strategies in Issatchenkia orientalis using constraint‐based modeling	5
Biotransformation of maize bran‐derived ferulic acid to vanillin using an adapted strain of Amycolatopsis sp. ATCC 39116	5
Modulation of high mannose levels in N‐linked glycosylation through cell culture process conditions to increase antibody‐dependent cell‐mediated cytotoxicity activity for an antibody biosimilar	5
Highly iterated palindrome 1 sequence improves Synechococcus sp. PCC 7002 transformation efficiencies in a homology‐ and methylation‐dependent manner	5
Characterization of Chinese hamster ovary cell culture feed media precipitate	5
Continuous CTC separation through a DEP‐based contraction–expansion inertial microfluidic channel	5
A deep‐well plate enabled automated high‐throughput cell line development platform	5
Genetically engineered neural stem cells (NSCs) therapy for neurological diseases; state‐of‐the‐art	5
CHOsynthetic promoters improve expression and product quality of biotherapeutic proteins	5
An automated high inoculation density fed‐batch bioreactor, enabled through N‐1 perfusion, accommodates clonal diversity and doubles titers	5
Process intensification of the baculovirus expression vector system using a perfusion process with a low multiplicity of infection at high cell concentrations	5
“Just add small molecules” cell‐free protein synthesis: CombiningDNAtemplate and cell extract preparation into a single fermentation	5
Advancing multiproduct resin reuse for development and clinical manufacturing of an antibody‐based therapeutic	5
Toward optimal clearance: A universal affinity‐based mass spectrometry approach for comprehensive ELISA reagent coverage evaluation and HCP hitchhiker analysis	5
A modular and multi‐functional purification strategy that enables a common framework for manufacturing scale integrated and continuous biomanufacturing	5
Modeling the effect of oxidative stress on Bordetella pertussis fermentations	5
Tangential flow filtration‐facilitated purification of human red blood cell membrane fragments and its preferential use in removing unencapsulated material from resealed red blood cell ghosts compared	4
Effect of culture hydrodynamics on Arthrospira platensis production using a single‐use photobioreactor system through a CFD supported approach	4
Size‐based analysis of virus removal filter fouling using fractionated protein aggregates	4
Sources and methods of manufacturing xanthan by fermentation of various carbon sources	4
Assessing the probability of clonality achieved by single‐cell cloning of CHO cells through cell deposition combined with imaging using distinguishable cells	4
Transcriptomic features reveal molecular signatures associated with recombinant adeno‐associated virus production in HEK293 cells	4
Smart process development: Application of machine‐learning and integrated process modeling for inclusion body purification processes	4
Evaluating end‐to‐end continuous antibody manufacture with column‐free capture alternatives from economic, environmental, and robustness perspectives	4
A strategy for successful dual‐species protein expression of genes with non‐optimal codon usage destined for bacterial and yeast cell factories	4
Identification of compendial nonionic detergents for the replacement of Triton X‐100 in bioprocessing	4
Advanced strategies in glycosylation prediction and control during biopharmaceutical development: Avenues toward industry 4.0	4
Bax and Bak knockout apoptosis‐resistant Chinese hamster ovary cell lines significantly improve culture viability and titer in intensified fed‐batch culture process	4
Efficient fermentative production of lactodifucotetraose by controlling sequential glycosyltransferase reactions in Escherichia coli	4
Exploring the potential of machine learning for more efficient development and production of biopharmaceuticals	4
Multi‐omics kinetic analysis of recombinant adeno‐associated virus production by plasmid transfection of HEK293 cells	4
Online monitored characterization of Phocaeicola vulgatus for organic acid production using anaerobic microtiter plate cultivations	4
The production of succinate with more CO₂ fixation reactions facilitated by RuBisCO‐based engineered Escherichia coli	4
Transitioning from static to suspension culture system for large‐scale production of xeno‐free extracellular vesicles derived from mesenchymal stromal cells	4
Yeast cells for encapsulation of bioactive compounds in food products: A review	4
Synthesis of a novel nanocomposite containing chitosan as a three‐dimensional printed wound dressing technique: Emphasis on gene expression	4
Rapid intensification of an established CHO cell fed‐batch process	4