OOIR: Observatory of International Research

Papers

(The TQCC of Biotechnology Progress is 5. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2022-05-01 to 2026-05-01.)

Article	Citations
Dynamic flux balance analysis of 1,3‐propanediol production by clostridium butyricum fermentation	82
From protein structure to an optimized chromatographic capture step using multiscale modeling	53
Design of experiments‐guided membrane capture facilitates integration of continuous virus inactivation using Phi6 as a surrogate virus in an intensified downstream process	51
Proteomics‐based method to comprehensively model the removal of host cell protein impurities	33
Optimized pulse‐feeding fed‐batch fermentation for enhanced lignin to polyhydroxyalkanoate transformation	30
Dynamic pH profiles drive higher cell‐specific and volumetric productivity	29
The effects of the chemical modification on immobilized lipase features are affected by the enzyme crowding in the support	29
Acetate accumulation and regulation by process parameters control in Chinese hamster ovary cell culture	29
Issue Information	27
High‐yield recombinant adeno‐associated viral vector production by multivariate optimization of bioprocess and transfection conditions	27
Pre‐folding purification procedures for inclusion body‐derived non‐tagged cationic recombinant proteins with multiple disulfide bonds for efficient refolding	26
A practical framework for clone selection, media‐feed screening, and upstream process parameter optimization for a mAb CHO cell culture process	25
Depth filtration for clarification of intensified lentiviral vector suspension cell culture	24
Enrichment of full AAV capsids by preparative strong anion exchange chromatography	23
Exploring the design space for T riton X ‐100 substitutes in viral inactivation applications	23
Issue Information	20
Removal of chromatin by salt‐tolerant endonucleases for production of recombinant measles virus	20
Evaluating multiproduct chromatography Protein A resin reuse for monoclonal antibodies in biopharmaceutical manufacturing	19
Current status of platelet manufacturing in 3D or bioreactors	19
Genetically engineered neural stem cells (NSCs) therapy for neurological diseases; state‐of‐the‐art	19
Online monitored characterization of Phocaeicola vulgatus for organic acid production using anaerobic microtiter plate cultivations	19
The production of succinate with more CO ₂ fixation reactions facilitate	18
Development of virus‐like particles‐based vaccines against coronaviruses	17
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Correlation of microbial community structure and volatile flavor compounds during corn yellow wine fermentation	17

Correction to “Investigation of the hepatic respiration and liver zonation on rat hepatocytes using an integrated oxygen biosensor in a microscale device”	17
Hands‐free from inoculation to harvest: Microbial fermentation with multivariate model to automate induction of recombinant protein expression	16
Wound dressing based on PVA nanofiber containing silk fibroin modified with GO /	16
Raman spectroscopy and one‐dimensional convolutional neural network modeling as a real‐time monitoring tool for in vitro transaminase‐catalyzed synthesis of a pharmaceutically relevant amine precursor	16
Unraveling the potential and constraints associated with corn steep liquor as a nutrient source for industrial fermentations	15
Sequential, chromosome‐specific glutamine synthetase double knockout with Cas‐ CLOVER establishes enhanced CHO	15
Modification of microstructure and selected physicochemical properties of bacterial cellulose produced by bacterial isolate using hydrocolloid‐fortified Hestrin–Schramm	15
Immobilized enzymes as potent antibiofilm agent	15
Antibody glycation during a Chinese hamster ovary fed‐batch process is following a constrained second order reaction	14
Automation of an integrated micro‐scale platform for monoclonal antibody process development by incorporation of a depth filter mimic	13
Non‐sterile cultivation of Yarrowia lipolytica in fed‐batch mode for the production of lipids and biomass	13
Improved endotoxin removal using ecofriendly detergents for intensified plasmid capture	13
Petroleum coke supplementation for enhanced biogas production and phosphate removal under mesophilic conditions	13
Characterization of lipoprotein supplement and influence of its oxidized lipid content on cell culture performance and monoclonal antibody production by a SP2	12
The development of an automated microscope image tracking and analysis system	12
Bioburden detection on surface and water samples in a rapid, ultra‐sensitive and high‐throughput manner	11
Enhanced production of isobutyl and isoamyl acetate using Yarrowia lipolytica	11
Enhancing mRNA vaccine production: Optimization of in vitro transcription for improved yield and purity	11
Accelerating IND‐enabling toxicology studies using protein products from stable pools or pools of clones in Chinese hamster ovary cells	10
Impact of X ‐ray irradiation as an equivalent alternative to gamma for sterilization of single‐use bioprocessing polymers	10
Primary clarification of mammalian cell culture fluid using enhanced sedimentation on inclined surfaces inside the single‐use disposable Sudhin BioSettler150	10
Enhancing CHO cell recombinant protein production using a perfusion‐directed host evolution approach	10
Improving trans‐cinnamic acid production in a model cyanobacterium	10
Effect of light fluctuations on photosynthesis and metabolic flux in Synechocystis sp. PCC 6803	10
	10
Shear stress and microRNAs for better metastatic cancer management	9
When will we have a clone? An industry perspective on the typical CLD timeline	9
Surface decoration of low molecular weight polyethylenimine ( LMW PEI ) by phthalated dextrin for improved delivery of interleukin‐12 plasmid	9
General strategies for IgG‐like bispecific antibody purification	9
A systemic approach to identifying sequence frameworks that decrease mAb production in a transient Chinese hamster ovary cel	9
Combining regulated and constitutive protein expression significantly boosts protein expression by increasing productivity without affecting CHO cell	9
A simple electrochemical genosensor based on polythiophene acetic acid film for detection of Schistosoma mansoni	9
Bioengineered chimeric tRNA / pre‐miRNAs as prodrugs in cancer therapy	9
Issue Information	8
Modeling scalability of impurity precipitation in downstream biomanufacturing	8
Non‐thermal plasma decontamination of microbes: a state of the art	8
Use of single analytic tool to quantify both absolute N‐glycosylation and glycan distribution in monoclonal antibodies	8
Enhancing cell separation in a hybrid spiral dielectrophoretic microchannel: Numerical insights and optimal operating conditions	8
Amino acid abundance and composition in cell culture medium affects trace metal tolerance and cholesterol synthesis	7
Comparison of multi‐column chromatography configurations through model‐based optimization	7
Issue Information	7
Optimization of amino acid composition in CHO cell perfusion medium using definitive screening design and	7
Biotechnological potential of yeast cell wall: An overview	7
High‐sensitivity real‐time monitoring of pH and respiration activity unveils metabolic dynamics in shake flask cultures	7
Metabolic engineering of rapidly growing Synechococcus elongatus strains for phototrophic producti	7
A size‐exclusion chromatography fingerprinting workflow for the development of flow‐through polishing operations for mAbs derived from continuous precipitation processes	7
Artificial intelligence and machine learning‐assisted digital applications for biopharmaceutical manufacturing	7
Transmission of poly( dT ₆₀ )	7
Comparing in silico flowsheet optimization strategies in biopharmaceutical downstream processes	7
A case study showing the role of hydrophobicity variants and other enriched mAb proteoforms on filterability through a virus filter with productivity	6

	6
Transcriptomic functional characterization of recombinant adeno‐associated virus producing cell line adapted to suspension‐growth	6
A strategy for successful dual‐species protein expression of genes with non‐optimal codon usage destined for bacterial and yeast cell factories	6
Impact of bioreactor process parameters and yeast biomass on Raman spectra	6
Exploring the potential of machine learning for more efficient development and production of biopharmaceuticals	6
Optimization of carotenoid production by Umbelopsis ramanniana	6
New technical concept for alternating tangential flow filtration in biotechnological cell separation processes	6
Issue Information	6
Process optimization mitigated the retention loss of an Fc‐fusion protein during ultrafiltration/diafiltration	6
Size‐based analysis of virus removal filter fouling using fractionated protein aggregates	6
Effects of LEDs, macronutrients and culture conditions on biomass and artemisinin production using Artemisia annuaL. suspension cultures	6
RETRACTION : Comparison of Expression Optimization of New Derivative of staphylokinase ( SAK ‐	6
A novel synthetic sRNA promoting protein overexpression in cell‐free systems	6
Hybrid modeling for in silico optimization of a dynamic perfusion cell culture process	6
Online measurement of the viscosity in shake flasks enables monitoring of γ‐PGA production in depolymerase knockout mutants of	6
An adaptive modeling approach using spiking‐augmentation method to improve chemometric model performance in bioprocess monitoring	6
Disulfide bond reduction of antibodies during clarification: A bench‐scale assay and a mitigation tool	6
Continuous glucose feedback control using Raman spectroscopy and deep learning models for biopharmaceutical processes	6
Antibody charge variant modulation by in vitro enzymatic treatment in different Chinese hamster ovary cell cultures	6
Predictive modeling of single pass tangential flow filtration for continuous biomanufacturing	6
Intensified perfusion culture (IPC) reduced recombinant protein fragmentation	5
Harnessing cell aggregates for enhanced adeno‐associated virus manufacturing: Cultivation strategies and scale‐up considerations	5
Pharmaceutical proteins at the interfaces and the role of albumin	5
Effect of cell retention techniques in Komagataella phaffii lab‐scale continuous processes	5
Utilizing C ole– C ole parameters for in‐line feedback: Cell culture process adjustments based on	5
Special issue on integrated continuous biomanufacturing	5
Purification of antisense oligonucleotides using hydrophobic interaction chromatography	5
A new method to immobilize urease in silk fibroin membrane by unidirectional nanopore dehydration	5
A systematic approach to reduce intraocular pressure for the treatment of glaucoma	5
Modification of C‐segment of Cfa DnaE split intein for improving	5
Polyhydroxyalkanoates and exopolysaccharides: An alternative for valuation of the co‐production of microbial biopolymers	5
Production of 8,11‐dihydroxy fatty acids from oleic and palmitoleic acids by Escherichia coli cells expressing variant 6,8‐linoleate diol synthases from	5
Issue Information	5
A synthetic platform for developing recombinant adeno‐associated virus type 8 producer cell lines	5
Modulation of nutrient precursors for controlling metabolic inhibitors by genome‐scale flux balance analysis	5
Issue Information	5
Design of a bactericidal hydrogel scaffold containing genipin crosslinked HF ‐18 peptide	5
Efficient optimization of time‐varying inputs in a fed‐batch cell culture process using design of dynamic experiments	5
Chitosan based hybrid superabsorbent for controlled drug delivery application	5